PI0007377 (gene) Melon (PI 482460) v1

BLAST of PI0007377 vs. ExPASy Swiss-Prot
Match: Q9T034 (Phenylalanine--tRNA ligase alpha subunit, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At4g39280 PE=2 SV=3)

HSP 1 Score: 765.0 bits (1974), Expect = 4.9e-220
Identity = 370/486 (76.13%), Postives = 415/486 (85.39%), Query Frame = 0

Query: 1   MAEEAILGYLEKHLEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN 60
           MAEEAILG+L+ + +I+DSG+F+ EF +DHNE+VNVIKSL+GFRY+D +DIK E W+LT+
Sbjct: 1   MAEEAILGFLQNNEQITDSGQFSAEFNIDHNELVNVIKSLHGFRYIDVQDIKKETWILTD 60

Query: 61  EGETYTATGSPEMQLFLAIPPEG-IPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMIS 120
           EG+ Y A GSPE+QLFLA+P EG I ++ELQKKL P++FKIGC+QA KNKWVEMGKQ +S
Sbjct: 61  EGKKYAAEGSPEVQLFLAVPEEGSISKDELQKKLAPAVFKIGCSQAGKNKWVEMGKQ-VS 120

Query: 121 RKVQHVEDKVKNSLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRRGPNYAPRRK-- 180
           RKV+ VEDKVK  LLQI+ GKE   E + +LKARKLIV Q WKGYSV++GPNYAP RK  
Sbjct: 121 RKVKDVEDKVKGQLLQIQQGKEFDKESINSLKARKLIVPQIWKGYSVKKGPNYAPERKKV 180

Query: 181 ------------RELEFKEYNFNAKGQPLLCG--------PTANKNIFRQMGFEEMPTNN 240
                       +ELEFKEYNF AKG P+  G            K+IF QMGFEEMPTNN
Sbjct: 181 ATDLTRENLQNWKELEFKEYNFKAKGAPVDAGHLHALLKVRKQFKDIFVQMGFEEMPTNN 240

Query: 241 YVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKGY 300
           YVESSFWNFDALFQPQQHPARD+HDTFFLK PSTTR LPEDYVERVK+VHE+GGYGS+GY
Sbjct: 241 YVESSFWNFDALFQPQQHPARDSHDTFFLKVPSTTRTLPEDYVERVKRVHESGGYGSRGY 300

Query: 301 GYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEFH 360
            Y+WKREEANKNLLRTHTTAV+SRMLY LAQKPF PKKYFSIDRVFRNEAVDRTHLAEFH
Sbjct: 301 NYDWKREEANKNLLRTHTTAVSSRMLYALAQKPFVPKKYFSIDRVFRNEAVDRTHLAEFH 360

Query: 361 QIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWVE 420
           QIEGLIC RGLTLGDLIGVL DFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEG  KWVE
Sbjct: 361 QIEGLICDRGLTLGDLIGVLEDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGLGKWVE 420

Query: 421 VGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKNP 464
           +GNSGMFRPEMLLPMG PED R+IAWGLSLERPTMI+YG+DNIRDLFGHKVDL L+K+NP
Sbjct: 421 IGNSGMFRPEMLLPMGLPEDVRVIAWGLSLERPTMILYGIDNIRDLFGHKVDLDLIKRNP 480

BLAST of PI0007377 vs. ExPASy Swiss-Prot
Match: Q505J8 (Phenylalanine--tRNA ligase alpha subunit OS=Rattus norvegicus OX=10116 GN=Farsa PE=1 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 4.9e-143
Identity = 265/504 (52.58%), Postives = 343/504 (68.06%), Query Frame = 0

Query: 1   MAEEAILGYLEKHLEISDSG----RFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEK- 60
           MA+  +L  L + LE++D G      A + G++H  VV  +KSL     V   +++  K 
Sbjct: 1   MADNPVLEQLLRRLEVADGGLDSAELATQLGVEHQAVVGAVKSLQALGEVIEAELRSTKC 60

Query: 61  WVLTNEGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGK 120
           W LT EGE     GS E ++F +IP EG+ + EL +   PS  K+G ++A  NKW+ + K
Sbjct: 61  WELTTEGEEIAREGSHEARVFRSIPLEGLVQSELMQL--PS-GKVGFSKAMSNKWIRVDK 120

Query: 121 Q-----MISRKVQHVEDKVKNSLLQIKDG--KEIYDEDVKALKARKLIVSQTWKGYSVRR 180
                  + R V  +ED+V+  L Q++ G  +++ +++   L+ RKL+     K Y V +
Sbjct: 121 SAADGPRVFRVVDSIEDEVQRRLQQVQAGQAEKLAEKERNELRKRKLLTEVILKTYWVSK 180

Query: 181 GPNYAPR-RKRELE---------------FKEYNFNAKG--------QPLLCGPTANKNI 240
           G  ++    K+E E               FK YNF+A+G         PLL   +  + I
Sbjct: 181 GKGFSTSVSKQEAELSPEMISSGSWRDRPFKPYNFSARGVLPDSGHLHPLLKVRSQFRQI 240

Query: 241 FRQMGFEEMPTNNYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVK 300
           F +MGF EMPT+N++ESSFWNFDALFQPQQHPARD HDTFFL+ P+   +LP DYV+RVK
Sbjct: 241 FLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMDYVQRVK 300

Query: 301 QVHETGGYGSKGYGYNWKREEANKNLLRTHTTAVTSRMLYMLAQ-KPFAPKKYFSIDRVF 360
           + H  GGYGS+GY Y WK EEA KNLLRTHTTA ++R LY LAQ KPF P KYFSIDRVF
Sbjct: 301 RTHSQGGYGSQGYKYTWKLEEARKNLLRTHTTAASARALYRLAQKKPFTPAKYFSIDRVF 360

Query: 361 RNEAVDRTHLAEFHQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSM 420
           RNE +D THLAEFHQIEG+I   GLTLG L+GVL +FF++LG+++LRFKPAYNPYTEPSM
Sbjct: 361 RNETLDATHLAEFHQIEGVIADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSM 420

Query: 421 EIFSYHEGFKKWVEVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDL 468
           E+FSYH+G KKWVEVGNSG+FRPEMLLPMG PE+  +IAWGLSLERPTMI YG++NIR+L
Sbjct: 421 EVFSYHQGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLERPTMIKYGINNIREL 480

BLAST of PI0007377 vs. ExPASy Swiss-Prot
Match: Q1JPX3 (Phenylalanine--tRNA ligase alpha subunit OS=Danio rerio OX=7955 GN=farsa PE=2 SV=2)

HSP 1 Score: 506.5 bits (1303), Expect = 3.2e-142
Identity = 260/498 (52.21%), Postives = 335/498 (67.27%), Query Frame = 0

Query: 1   MAEEAILGYLEKHLEISDSGRFADE----FGLDHNEVVNVIKSLNGF-RYVDAEDIKMEK 60
           MA+  +L  L + +E  D G  + +     G+DH  VV  +KSL      + AE    + 
Sbjct: 1   MADTGLLEALLQRVEQLDGGVDSQDVSAALGVDHQLVVGAVKSLQALGEVISAEQKSSKH 60

Query: 61  WVLTNEGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEM-- 120
           W LT EG      GS E ++F AIP EG+P+ +L K       K+G ++A  NKW+ +  
Sbjct: 61  WELTGEGREIAEQGSHEARVFNAIPAEGLPQNQLMKMASG---KVGFSKAMSNKWIRLDK 120

Query: 121 ---GKQMISRKVQHVEDKVKNSLLQIKDGK--EIYDEDVKALKARKLIVSQTWKGYSVRR 180
              G   + R V+ +ED V++ L  +++G+  ++ +++   LK RKL+   T K Y + +
Sbjct: 121 AHEGGPRVFRTVESIEDTVRDKLQLVQNGQSAKLEEKEKNELKKRKLLAEVTVKSYWITK 180

Query: 181 GPNY----------------APRRKRELEFKEYNFNAKG--------QPLLCGPTANKNI 240
           G ++                A    +E +FK YNF A G         PL+   T  + I
Sbjct: 181 GNSFSTTITKQETELTPEMIASGNWKEKKFKPYNFEAMGVAPDCGHLHPLMKVRTQFRQI 240

Query: 241 FRQMGFEEMPTNNYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVK 300
           F +MGF EMPTNN++ESSFWNFD+LFQPQQHPARD HDTFF+  P+   E P DY+ERVK
Sbjct: 241 FLEMGFTEMPTNNFIESSFWNFDSLFQPQQHPARDQHDTFFISDPALAHEFPRDYLERVK 300

Query: 301 QVHETGGYGSKGYGYNWKREEANKNLLRTHTTAVTSRMLYMLA-QKPFAPKKYFSIDRVF 360
           +VH  GGYGS+GY Y+WK EEA KNLLRTHTTAV++RMLY LA Q+ F P KYFSIDRVF
Sbjct: 301 KVHSEGGYGSQGYKYDWKIEEAQKNLLRTHTTAVSARMLYKLAQQEKFTPVKYFSIDRVF 360

Query: 361 RNEAVDRTHLAEFHQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSM 420
           RNE +D THLAEFHQIEG++   GLTLG+L+GVL+ FF++LG++KLRFKPAYNPYTEPSM
Sbjct: 361 RNETLDATHLAEFHQIEGVVADYGLTLGNLMGVLHQFFTKLGITKLRFKPAYNPYTEPSM 420

Query: 421 EIFSYHEGFKKWVEVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDL 462
           E+FSYHEG KKWVEVGNSG+FRPEMLLPMG PE   +IAWGLSLERPTMI YG++NIR+L
Sbjct: 421 EVFSYHEGLKKWVEVGNSGVFRPEMLLPMGLPEGVSVIAWGLSLERPTMIKYGINNIREL 480

BLAST of PI0007377 vs. ExPASy Swiss-Prot
Match: Q9Y285 (Phenylalanine--tRNA ligase alpha subunit OS=Homo sapiens OX=9606 GN=FARSA PE=1 SV=3)

HSP 1 Score: 502.7 bits (1293), Expect = 4.6e-141
Identity = 263/503 (52.29%), Postives = 339/503 (67.40%), Query Frame = 0

Query: 1   MAEEAILGYLEKHLEISDSG----RFADEFGLDHNEVVNVIKSLNGF-RYVDAEDIKMEK 60
           MA+  +   L + LE SD G      A E G++H  VV  +KSL      ++AE    + 
Sbjct: 1   MADGQVAELLLRRLEASDGGLDSAELAAELGMEHQAVVGAVKSLQALGEVIEAELRSTKH 60

Query: 61  WVLTNEGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGK 120
           W LT EGE     GS E ++F +IPPEG+ + EL +   PS  K+G ++A  NKW+ + K
Sbjct: 61  WELTAEGEEIAREGSHEARVFRSIPPEGLAQSELMRL--PS-GKVGFSKAMSNKWIRVDK 120

Query: 121 Q-----MISRKVQHVEDKVKNSLLQIKDG--KEIYDEDVKALKARKLIVSQTWKGYSVRR 180
                  + R V  +ED+V+  L  ++ G  +++ +++   L+ RKL+   T K Y V +
Sbjct: 121 SAADGPRVFRVVDSMEDEVQRRLQLVRGGQAEKLGEKERSELRKRKLLAEVTLKTYWVSK 180

Query: 181 GPNYAPR-RKRELE---------------FKEYNFNAKG--------QPLLCGPTANKNI 240
           G  ++    K+E E               FK YNF A G         PLL   +  + I
Sbjct: 181 GSAFSTSISKQETELSPEMISSGSWRDRPFKPYNFLAHGVLPDSGHLHPLLKVRSQFRQI 240

Query: 241 FRQMGFEEMPTNNYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVK 300
           F +MGF EMPT+N++ESSFWNFDALFQPQQHPARD HDTFFL+ P+   +LP DYV+RVK
Sbjct: 241 FLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMDYVQRVK 300

Query: 301 QVHETGGYGSKGYGYNWKREEANKNLLRTHTTAVTSRMLYMLAQ-KPFAPKKYFSIDRVF 360
           + H  GGYGS+GY YNWK +EA KNLLRTHTT+ ++R LY LAQ KPF P KYFSIDRVF
Sbjct: 301 RTHSQGGYGSQGYKYNWKLDEARKNLLRTHTTSASARALYRLAQKKPFTPVKYFSIDRVF 360

Query: 361 RNEAVDRTHLAEFHQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSM 420
           RNE +D THLAEFHQIEG++   GLTLG L+GVL +FF++LG+++LRFKPAYNPYTEPSM
Sbjct: 361 RNETLDATHLAEFHQIEGVVADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSM 420

Query: 421 EIFSYHEGFKKWVEVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDL 467
           E+FSYH+G KKWVEVGNSG+FRPEMLLPMG PE+  +IAWGLSLERPTMI YG++NIR+L
Sbjct: 421 EVFSYHQGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLERPTMIKYGINNIREL 480

BLAST of PI0007377 vs. ExPASy Swiss-Prot
Match: Q8C0C7 (Phenylalanine--tRNA ligase alpha subunit OS=Mus musculus OX=10090 GN=Farsa PE=1 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 4.6e-141
Identity = 263/504 (52.18%), Postives = 339/504 (67.26%), Query Frame = 0

Query: 1   MAEEAILGYLEKHLEISDSG----RFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEK- 60
           MA+  +L  L + LE++D G      A + G++H  VV  +KSL     V   +++  K 
Sbjct: 1   MADNPVLELLLRRLEVADGGLDSAELATQLGVEHQAVVGAVKSLQALGEVIEAELRSTKC 60

Query: 61  WVLTNEGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGK 120
           W LT EGE     GS E ++F +IP EG+ + EL     PS  K+G ++A  NKW+ + K
Sbjct: 61  WELTTEGEEIAREGSHEARVFRSIPLEGLVQSELMHL--PS-GKVGFSKAMSNKWIRVDK 120

Query: 121 Q-----MISRKVQHVEDKVKN--SLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRR 180
                  + R V  +ED+V+    L+Q    +++ +++   L+ RKL+     K Y V +
Sbjct: 121 SAADGPRVFRVVDSIEDEVQKRLQLVQAGQAEKLAEKERNELRKRKLLTEVILKTYWVSK 180

Query: 181 GPNYAPR-RKRELE---------------FKEYNFNAKG--------QPLLCGPTANKNI 240
           G  ++    K+E E               FK YNF+A+G         PLL   +  + I
Sbjct: 181 GKAFSTSVSKQEAELSPEMISSGSWRDRPFKPYNFSARGVLPDSGHLHPLLKVRSQFRQI 240

Query: 241 FRQMGFEEMPTNNYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVK 300
           F +MGF EMPT+N++ESSFWNFDALFQPQQHPARD HDTFFL+ P+   +LP  YV+RVK
Sbjct: 241 FLEMGFTEMPTDNFIESSFWNFDALFQPQQHPARDQHDTFFLRDPAEALQLPMGYVQRVK 300

Query: 301 QVHETGGYGSKGYGYNWKREEANKNLLRTHTTAVTSRMLYMLAQ-KPFAPKKYFSIDRVF 360
           + H  GGYGS+GY Y WK EEA KNLLRTHTTA ++R LY LAQ KPF P KYFSIDRVF
Sbjct: 301 RTHSQGGYGSQGYKYTWKLEEARKNLLRTHTTAASARALYQLAQKKPFTPAKYFSIDRVF 360

Query: 361 RNEAVDRTHLAEFHQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSM 420
           RNE +D THLAEFHQIEG+I   GLTLG L+GVL +FF++LG+++LRFKPAYNPYTEPSM
Sbjct: 361 RNETLDATHLAEFHQIEGVIADHGLTLGHLMGVLREFFTKLGITQLRFKPAYNPYTEPSM 420

Query: 421 EIFSYHEGFKKWVEVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDL 468
           E+FSYH+G KKWVEVGNSG+FRPEMLLPMG PE+  +IAWGLSLERPTMI YG++NIR+L
Sbjct: 421 EVFSYHQGLKKWVEVGNSGVFRPEMLLPMGLPENVSVIAWGLSLERPTMIKYGINNIREL 480

BLAST of PI0007377 vs. ExPASy TrEMBL
Match: A0A5D3CJP1 (Phenylalanine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001880 PE=3 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 6.2e-266
Identity = 460/492 (93.50%), Postives = 461/492 (93.70%), Query Frame = 0

Query: 1   MAEEAILGYLEKHLEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN 60
           MAEEAILGYLEK+ EISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN
Sbjct: 1   MAEEAILGYLEKNPEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN 60

Query: 61  EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR 120
           EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR
Sbjct: 61  EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR 120

Query: 121 KVQHVEDKVKNSLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRRGPNYAPRRK--- 180
           KV HVEDKVKN LLQIKDGKEIYDEDVKALKARKLIVSQTWKGY VRRGPNYAPRRK   
Sbjct: 121 KVHHVEDKVKNLLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYLVRRGPNYAPRRKKVA 180

Query: 181 -------------RELEFKEYNFNAKGQPLLCG---PTAN-----KNIFRQMGFEEMPTN 240
                        RELEFKEYNFNAKGQPLLCG   P        KNIFRQMGFEEMPTN
Sbjct: 181 TDLTRENLQRGDWRELEFKEYNFNAKGQPLLCGHLHPLLKVRQQIKNIFRQMGFEEMPTN 240

Query: 241 NYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKG 300
           NYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKG
Sbjct: 241 NYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKG 300

Query: 301 YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360
           YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF
Sbjct: 301 YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360

Query: 361 HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420
           HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV
Sbjct: 361 HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420

Query: 421 EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKN 469
           EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKN
Sbjct: 421 EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKN 480

BLAST of PI0007377 vs. ExPASy TrEMBL
Match: A0A1S3BKE3 (Phenylalanine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103490811 PE=3 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 6.2e-266
Identity = 460/492 (93.50%), Postives = 461/492 (93.70%), Query Frame = 0

Query: 1   MAEEAILGYLEKHLEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN 60
           MAEEAILGYLEK+ EISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN
Sbjct: 1   MAEEAILGYLEKNPEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN 60

Query: 61  EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR 120
           EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR
Sbjct: 61  EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR 120

Query: 121 KVQHVEDKVKNSLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRRGPNYAPRRK--- 180
           KV HVEDKVKN LLQIKDGKEIYDEDVKALKARKLIVSQTWKGY VRRGPNYAPRRK   
Sbjct: 121 KVHHVEDKVKNLLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYLVRRGPNYAPRRKKVA 180

Query: 181 -------------RELEFKEYNFNAKGQPLLCG---PTAN-----KNIFRQMGFEEMPTN 240
                        RELEFKEYNFNAKGQPLLCG   P        KNIFRQMGFEEMPTN
Sbjct: 181 TDLTRENLQRGDWRELEFKEYNFNAKGQPLLCGHLHPLLKVRQQIKNIFRQMGFEEMPTN 240

Query: 241 NYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKG 300
           NYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKG
Sbjct: 241 NYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKG 300

Query: 301 YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360
           YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF
Sbjct: 301 YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360

Query: 361 HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420
           HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV
Sbjct: 361 HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420

Query: 421 EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKN 469
           EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKN
Sbjct: 421 EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKN 480

BLAST of PI0007377 vs. ExPASy TrEMBL
Match: A0A0A0L425 (Phenylalanine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_3G016390 PE=3 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 1.8e-265
Identity = 457/492 (92.89%), Postives = 460/492 (93.50%), Query Frame = 0

Query: 1   MAEEAILGYLEKHLEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN 60
           MAEEAILGYLEK+ EISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLT 
Sbjct: 1   MAEEAILGYLEKNTEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTK 60

Query: 61  EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR 120
           EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR
Sbjct: 61  EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR 120

Query: 121 KVQHVEDKVKNSLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRRGPNYAPRRK--- 180
           KV HVEDKVKNSLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRRGPNYAPRRK   
Sbjct: 121 KVHHVEDKVKNSLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRRGPNYAPRRKKVA 180

Query: 181 -------------RELEFKEYNFNAKGQPLLCG---PTAN-----KNIFRQMGFEEMPTN 240
                        RELEFKEYNFNAKGQPL CG   P        KNIFRQMGFEEMPTN
Sbjct: 181 TDLTRENLQRGDWRELEFKEYNFNAKGQPLFCGHLHPLLKVRQQIKNIFRQMGFEEMPTN 240

Query: 241 NYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKG 300
           NYVESSFWNFD LFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGS+G
Sbjct: 241 NYVESSFWNFDTLFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSRG 300

Query: 301 YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360
           YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF
Sbjct: 301 YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360

Query: 361 HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420
           HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV
Sbjct: 361 HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420

Query: 421 EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKN 469
           EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYG+DNIRDLFGHKVDLSLVKKN
Sbjct: 421 EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGIDNIRDLFGHKVDLSLVKKN 480

BLAST of PI0007377 vs. ExPASy TrEMBL
Match: A0A6J1E9L5 (Phenylalanine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111431119 PE=3 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 2.5e-251
Identity = 429/489 (87.73%), Postives = 452/489 (92.43%), Query Frame = 0

Query: 1   MAEEAILGYLEKHLEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN 60
           MAEEAILGYLEK++EISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIK EKW+LT+
Sbjct: 1   MAEEAILGYLEKNVEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKKEKWILTD 60

Query: 61  EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR 120
           EGETYTATGSPE+QLFLAIPPEGIP+EELQKKLGPSIFKIGCAQAAKNKWVEMGKQ ISR
Sbjct: 61  EGETYTATGSPELQLFLAIPPEGIPKEELQKKLGPSIFKIGCAQAAKNKWVEMGKQSISR 120

Query: 121 KVQHVEDKVKNSLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRRGPNYAPRRK--- 180
           KVQHVEDKVKNSLLQIKDGKE++D+DVKALKARKLIVSQTWKGYSV++GP+YAPRRK   
Sbjct: 121 KVQHVEDKVKNSLLQIKDGKEVHDDDVKALKARKLIVSQTWKGYSVKKGPSYAPRRKKAA 180

Query: 181 -------------RELEFKEYNFNAKGQPLLCG---PTAN-----KNIFRQMGFEEMPTN 240
                        RELEFKEYNFNAKGQPL  G   P        KNIFRQMGFEEMPTN
Sbjct: 181 TDLTRENLQRGDWRELEFKEYNFNAKGQPLEGGHLHPLLKVRQQLKNIFRQMGFEEMPTN 240

Query: 241 NYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKG 300
           N+VESSFWNFDALFQPQQHPARDAHDTFFL+AP++TRELPEDYVERVKQVHE GG+GSKG
Sbjct: 241 NFVESSFWNFDALFQPQQHPARDAHDTFFLQAPASTRELPEDYVERVKQVHEIGGFGSKG 300

Query: 301 YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360
           YGY+WKREEANKNLLRTHTTAV+SRMLY+LAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF
Sbjct: 301 YGYDWKREEANKNLLRTHTTAVSSRMLYLLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360

Query: 361 HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420
           HQIEGLIC RGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV
Sbjct: 361 HQIEGLICDRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420

Query: 421 EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKN 466
           EVGNSGMFRPEMLLPMG PED R+IAWGLSLERPTMI+YGVDNIRDLFGHKVDL L+KKN
Sbjct: 421 EVGNSGMFRPEMLLPMGLPEDVRVIAWGLSLERPTMILYGVDNIRDLFGHKVDLGLMKKN 480

BLAST of PI0007377 vs. ExPASy TrEMBL
Match: A0A6J1L3Z0 (Phenylalanine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111500296 PE=3 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 2.1e-250
Identity = 428/489 (87.53%), Postives = 451/489 (92.23%), Query Frame = 0

Query: 1   MAEEAILGYLEKHLEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN 60
           MAEEAILGYLEK++EISDSGRFADEF LDHNEVVNVIKSLNGFRYVDAEDIK EKW+LT+
Sbjct: 1   MAEEAILGYLEKNVEISDSGRFADEFRLDHNEVVNVIKSLNGFRYVDAEDIKKEKWILTD 60

Query: 61  EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR 120
           EGETYTATGSPE+QLFLAIPPEGIP+EELQKKLGPSIFKIGCAQAAKNKWVEMGKQ ISR
Sbjct: 61  EGETYTATGSPELQLFLAIPPEGIPKEELQKKLGPSIFKIGCAQAAKNKWVEMGKQSISR 120

Query: 121 KVQHVEDKVKNSLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRRGPNYAPRRK--- 180
           KVQHVEDKVKNSLLQIKDGKE++D+DVKALKARKLIVSQTWKGYSV++GP+YAPRRK   
Sbjct: 121 KVQHVEDKVKNSLLQIKDGKEVHDDDVKALKARKLIVSQTWKGYSVKKGPSYAPRRKKAA 180

Query: 181 -------------RELEFKEYNFNAKGQPLLCG---PTAN-----KNIFRQMGFEEMPTN 240
                        RELEFKEYNFNAKGQPL  G   P        KNIFRQMGFEEMPTN
Sbjct: 181 TDLTRENLQRGDWRELEFKEYNFNAKGQPLEGGHLHPLLKVRQQLKNIFRQMGFEEMPTN 240

Query: 241 NYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKG 300
           N+VESSFWNFDALFQPQQHPARDAHDTFFL+AP++TRELPEDYVERVKQVHE GG+GSKG
Sbjct: 241 NFVESSFWNFDALFQPQQHPARDAHDTFFLQAPASTRELPEDYVERVKQVHEIGGFGSKG 300

Query: 301 YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360
           YGY+WKREEANKNLLRTHTTAV+SRMLY+LAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF
Sbjct: 301 YGYDWKREEANKNLLRTHTTAVSSRMLYLLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360

Query: 361 HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420
           HQIEGLIC RGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV
Sbjct: 361 HQIEGLICDRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420

Query: 421 EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKN 466
           EVGNSGMFRPEMLLPMG PED R+IAWGLSLERPTMI+YGVDNIRDLFGHKVDL L+KKN
Sbjct: 421 EVGNSGMFRPEMLLPMGLPEDVRVIAWGLSLERPTMILYGVDNIRDLFGHKVDLGLMKKN 480

BLAST of PI0007377 vs. NCBI nr
Match: XP_008448735.1 (PREDICTED: phenylalanine--tRNA ligase alpha subunit, cytoplasmic [Cucumis melo] >KAA0053067.1 phenylalanine--tRNA ligase alpha subunit [Cucumis melo var. makuwa] >TYK11522.1 phenylalanine--tRNA ligase alpha subunit [Cucumis melo var. makuwa])

HSP 1 Score: 926.0 bits (2392), Expect = 1.3e-265
Identity = 460/492 (93.50%), Postives = 461/492 (93.70%), Query Frame = 0

Query: 1   MAEEAILGYLEKHLEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN 60
           MAEEAILGYLEK+ EISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN
Sbjct: 1   MAEEAILGYLEKNPEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN 60

Query: 61  EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR 120
           EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR
Sbjct: 61  EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR 120

Query: 121 KVQHVEDKVKNSLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRRGPNYAPRRK--- 180
           KV HVEDKVKN LLQIKDGKEIYDEDVKALKARKLIVSQTWKGY VRRGPNYAPRRK   
Sbjct: 121 KVHHVEDKVKNLLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYLVRRGPNYAPRRKKVA 180

Query: 181 -------------RELEFKEYNFNAKGQPLLCG---PTAN-----KNIFRQMGFEEMPTN 240
                        RELEFKEYNFNAKGQPLLCG   P        KNIFRQMGFEEMPTN
Sbjct: 181 TDLTRENLQRGDWRELEFKEYNFNAKGQPLLCGHLHPLLKVRQQIKNIFRQMGFEEMPTN 240

Query: 241 NYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKG 300
           NYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKG
Sbjct: 241 NYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKG 300

Query: 301 YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360
           YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF
Sbjct: 301 YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360

Query: 361 HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420
           HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV
Sbjct: 361 HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420

Query: 421 EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKN 469
           EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKN
Sbjct: 421 EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKN 480

BLAST of PI0007377 vs. NCBI nr
Match: XP_004150173.1 (phenylalanine--tRNA ligase alpha subunit, cytoplasmic [Cucumis sativus] >KGN55794.1 hypothetical protein Csa_010065 [Cucumis sativus])

HSP 1 Score: 924.5 bits (2388), Expect = 3.7e-265
Identity = 457/492 (92.89%), Postives = 460/492 (93.50%), Query Frame = 0

Query: 1   MAEEAILGYLEKHLEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN 60
           MAEEAILGYLEK+ EISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLT 
Sbjct: 1   MAEEAILGYLEKNTEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTK 60

Query: 61  EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR 120
           EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR
Sbjct: 61  EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR 120

Query: 121 KVQHVEDKVKNSLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRRGPNYAPRRK--- 180
           KV HVEDKVKNSLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRRGPNYAPRRK   
Sbjct: 121 KVHHVEDKVKNSLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRRGPNYAPRRKKVA 180

Query: 181 -------------RELEFKEYNFNAKGQPLLCG---PTAN-----KNIFRQMGFEEMPTN 240
                        RELEFKEYNFNAKGQPL CG   P        KNIFRQMGFEEMPTN
Sbjct: 181 TDLTRENLQRGDWRELEFKEYNFNAKGQPLFCGHLHPLLKVRQQIKNIFRQMGFEEMPTN 240

Query: 241 NYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKG 300
           NYVESSFWNFD LFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGS+G
Sbjct: 241 NYVESSFWNFDTLFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSRG 300

Query: 301 YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360
           YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF
Sbjct: 301 YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360

Query: 361 HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420
           HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV
Sbjct: 361 HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420

Query: 421 EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKN 469
           EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYG+DNIRDLFGHKVDLSLVKKN
Sbjct: 421 EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGIDNIRDLFGHKVDLSLVKKN 480

BLAST of PI0007377 vs. NCBI nr
Match: XP_038905221.1 (phenylalanine--tRNA ligase alpha subunit, cytoplasmic [Benincasa hispida])

HSP 1 Score: 894.4 bits (2310), Expect = 4.1e-256
Identity = 440/492 (89.43%), Postives = 455/492 (92.48%), Query Frame = 0

Query: 1   MAEEAILGYLEKHLEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN 60
           MAEEAIL YLEK++EISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN
Sbjct: 1   MAEEAILSYLEKNVEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN 60

Query: 61  EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR 120
           EGETYTA GSPE+QLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR
Sbjct: 61  EGETYTAAGSPEVQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR 120

Query: 121 KVQHVEDKVKNSLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRRGPNYAPRRK--- 180
           KVQHV+DKVKNSLL+IK+GKE+Y+EDVK LKARKLIVSQTWKGYSV++GPNYAPRRK   
Sbjct: 121 KVQHVDDKVKNSLLRIKNGKEVYEEDVKGLKARKLIVSQTWKGYSVKKGPNYAPRRKKVA 180

Query: 181 -------------RELEFKEYNFNAKGQPLLCG---PTAN-----KNIFRQMGFEEMPTN 240
                        RELEFKEYNFNAKGQP+L G   P        KNIFRQMGFEEMPTN
Sbjct: 181 TDLTRENLQRGDWRELEFKEYNFNAKGQPVLGGHLHPLLKVRQQIKNIFRQMGFEEMPTN 240

Query: 241 NYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKG 300
           NYVESSFWNFDALFQPQQHPARDAHDTFFLK PSTTRELPEDYV+RVKQVHETGGYGSKG
Sbjct: 241 NYVESSFWNFDALFQPQQHPARDAHDTFFLKDPSTTRELPEDYVDRVKQVHETGGYGSKG 300

Query: 301 YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360
           YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF
Sbjct: 301 YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360

Query: 361 HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420
           HQIEGLIC R  TLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV
Sbjct: 361 HQIEGLICDRDRTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420

Query: 421 EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKN 469
           EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMI+YGVDNIRDLFGHKVDL LVKKN
Sbjct: 421 EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMILYGVDNIRDLFGHKVDLGLVKKN 480

BLAST of PI0007377 vs. NCBI nr
Match: KAG6577636.1 (Phenylalanine--tRNA ligase alpha subunit, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 877.5 bits (2266), Expect = 5.2e-251
Identity = 428/489 (87.53%), Postives = 453/489 (92.64%), Query Frame = 0

Query: 1   MAEEAILGYLEKHLEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN 60
           MAEEAILGYLEK++EISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIK EKW+LT+
Sbjct: 1   MAEEAILGYLEKNVEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKKEKWILTD 60

Query: 61  EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR 120
           EGETYTATGSPE+QLFLAIPPEGIP+EELQKKLGPSIFKIGCAQAAKNKWVEMGKQ+ISR
Sbjct: 61  EGETYTATGSPELQLFLAIPPEGIPKEELQKKLGPSIFKIGCAQAAKNKWVEMGKQLISR 120

Query: 121 KVQHVEDKVKNSLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRRGPNYAPRRK--- 180
           KVQHVEDKVKNSLLQIKDGKE++D+DVKALKARKLIVSQTWKGYSV++GP+YAPRRK   
Sbjct: 121 KVQHVEDKVKNSLLQIKDGKEVHDDDVKALKARKLIVSQTWKGYSVKKGPSYAPRRKKAA 180

Query: 181 -------------RELEFKEYNFNAKGQPLLCG---PTAN-----KNIFRQMGFEEMPTN 240
                        RELEFKEYNFNAKGQPL  G   P        KNIFRQMGFEEMPTN
Sbjct: 181 TDLTRENLQRGDWRELEFKEYNFNAKGQPLEGGHLHPLLKVRQQLKNIFRQMGFEEMPTN 240

Query: 241 NYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKG 300
           N+VESSFWNFDALFQPQQHPARDAHDTFFL+AP++TRELPEDYVERVKQVHE GG+GSKG
Sbjct: 241 NFVESSFWNFDALFQPQQHPARDAHDTFFLQAPASTRELPEDYVERVKQVHEIGGFGSKG 300

Query: 301 YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360
           YGY+WKREEANKNLLRTHTTAV+SRMLY+LAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF
Sbjct: 301 YGYDWKREEANKNLLRTHTTAVSSRMLYLLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360

Query: 361 HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420
           HQIEGLIC RGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV
Sbjct: 361 HQIEGLICDRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420

Query: 421 EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKN 466
           EVGNSGMFRPEMLLPMG P+D R+IAWGLSLERPTMI+YGVDNIRDLFGHKVDL L+KKN
Sbjct: 421 EVGNSGMFRPEMLLPMGLPDDVRVIAWGLSLERPTMILYGVDNIRDLFGHKVDLGLMKKN 480

BLAST of PI0007377 vs. NCBI nr
Match: XP_022923418.1 (phenylalanine--tRNA ligase alpha subunit, cytoplasmic-like [Cucurbita moschata] >XP_023553037.1 phenylalanine--tRNA ligase alpha subunit, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 877.5 bits (2266), Expect = 5.2e-251
Identity = 429/489 (87.73%), Postives = 452/489 (92.43%), Query Frame = 0

Query: 1   MAEEAILGYLEKHLEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN 60
           MAEEAILGYLEK++EISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIK EKW+LT+
Sbjct: 1   MAEEAILGYLEKNVEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKKEKWILTD 60

Query: 61  EGETYTATGSPEMQLFLAIPPEGIPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMISR 120
           EGETYTATGSPE+QLFLAIPPEGIP+EELQKKLGPSIFKIGCAQAAKNKWVEMGKQ ISR
Sbjct: 61  EGETYTATGSPELQLFLAIPPEGIPKEELQKKLGPSIFKIGCAQAAKNKWVEMGKQSISR 120

Query: 121 KVQHVEDKVKNSLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRRGPNYAPRRK--- 180
           KVQHVEDKVKNSLLQIKDGKE++D+DVKALKARKLIVSQTWKGYSV++GP+YAPRRK   
Sbjct: 121 KVQHVEDKVKNSLLQIKDGKEVHDDDVKALKARKLIVSQTWKGYSVKKGPSYAPRRKKAA 180

Query: 181 -------------RELEFKEYNFNAKGQPLLCG---PTAN-----KNIFRQMGFEEMPTN 240
                        RELEFKEYNFNAKGQPL  G   P        KNIFRQMGFEEMPTN
Sbjct: 181 TDLTRENLQRGDWRELEFKEYNFNAKGQPLEGGHLHPLLKVRQQLKNIFRQMGFEEMPTN 240

Query: 241 NYVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKG 300
           N+VESSFWNFDALFQPQQHPARDAHDTFFL+AP++TRELPEDYVERVKQVHE GG+GSKG
Sbjct: 241 NFVESSFWNFDALFQPQQHPARDAHDTFFLQAPASTRELPEDYVERVKQVHEIGGFGSKG 300

Query: 301 YGYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360
           YGY+WKREEANKNLLRTHTTAV+SRMLY+LAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF
Sbjct: 301 YGYDWKREEANKNLLRTHTTAVSSRMLYLLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEF 360

Query: 361 HQIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420
           HQIEGLIC RGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV
Sbjct: 361 HQIEGLICDRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWV 420

Query: 421 EVGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKN 466
           EVGNSGMFRPEMLLPMG PED R+IAWGLSLERPTMI+YGVDNIRDLFGHKVDL L+KKN
Sbjct: 421 EVGNSGMFRPEMLLPMGLPEDVRVIAWGLSLERPTMILYGVDNIRDLFGHKVDLGLMKKN 480

BLAST of PI0007377 vs. TAIR 10
Match: AT4G39280.1 (phenylalanyl-tRNA synthetase, putative / phenylalanine--tRNA ligase, putative )

HSP 1 Score: 765.0 bits (1974), Expect = 3.5e-221
Identity = 370/486 (76.13%), Postives = 415/486 (85.39%), Query Frame = 0

Query: 1   MAEEAILGYLEKHLEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN 60
           MAEEAILG+L+ + +I+DSG+F+ EF +DHNE+VNVIKSL+GFRY+D +DIK E W+LT+
Sbjct: 1   MAEEAILGFLQNNEQITDSGQFSAEFNIDHNELVNVIKSLHGFRYIDVQDIKKETWILTD 60

Query: 61  EGETYTATGSPEMQLFLAIPPEG-IPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMIS 120
           EG+ Y A GSPE+QLFLA+P EG I ++ELQKKL P++FKIGC+QA KNKWVEMGKQ +S
Sbjct: 61  EGKKYAAEGSPEVQLFLAVPEEGSISKDELQKKLAPAVFKIGCSQAGKNKWVEMGKQ-VS 120

Query: 121 RKVQHVEDKVKNSLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRRGPNYAPRRK-- 180
           RKV+ VEDKVK  LLQI+ GKE   E + +LKARKLIV Q WKGYSV++GPNYAP RK  
Sbjct: 121 RKVKDVEDKVKGQLLQIQQGKEFDKESINSLKARKLIVPQIWKGYSVKKGPNYAPERKKV 180

Query: 181 ------------RELEFKEYNFNAKGQPLLCG--------PTANKNIFRQMGFEEMPTNN 240
                       +ELEFKEYNF AKG P+  G            K+IF QMGFEEMPTNN
Sbjct: 181 ATDLTRENLQNWKELEFKEYNFKAKGAPVDAGHLHALLKVRKQFKDIFVQMGFEEMPTNN 240

Query: 241 YVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKGY 300
           YVESSFWNFDALFQPQQHPARD+HDTFFLK PSTTR LPEDYVERVK+VHE+GGYGS+GY
Sbjct: 241 YVESSFWNFDALFQPQQHPARDSHDTFFLKVPSTTRTLPEDYVERVKRVHESGGYGSRGY 300

Query: 301 GYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEFH 360
            Y+WKREEANKNLLRTHTTAV+SRMLY LAQKPF PKKYFSIDRVFRNEAVDRTHLAEFH
Sbjct: 301 NYDWKREEANKNLLRTHTTAVSSRMLYALAQKPFVPKKYFSIDRVFRNEAVDRTHLAEFH 360

Query: 361 QIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWVE 420
           QIEGLIC RGLTLGDLIGVL DFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEG  KWVE
Sbjct: 361 QIEGLICDRGLTLGDLIGVLEDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGLGKWVE 420

Query: 421 VGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKNP 464
           +GNSGMFRPEMLLPMG PED R+IAWGLSLERPTMI+YG+DNIRDLFGHKVDL L+K+NP
Sbjct: 421 IGNSGMFRPEMLLPMGLPEDVRVIAWGLSLERPTMILYGIDNIRDLFGHKVDLDLIKRNP 480

BLAST of PI0007377 vs. TAIR 10
Match: AT4G39280.2 (phenylalanyl-tRNA synthetase, putative / phenylalanine--tRNA ligase, putative )

HSP 1 Score: 764.6 bits (1973), Expect = 4.6e-221
Identity = 370/486 (76.13%), Postives = 415/486 (85.39%), Query Frame = 0

Query: 1   MAEEAILGYLEKHLEISDSGRFADEFGLDHNEVVNVIKSLNGFRYVDAEDIKMEKWVLTN 60
           MAEEAILG+L+ + +I+DSG+F+ EF +DHNE+VNVIKSL+GFRY+D +DIK E W+LT+
Sbjct: 1   MAEEAILGFLQNNEQITDSGQFSAEFNIDHNELVNVIKSLHGFRYIDVQDIKKETWILTD 60

Query: 61  EGETYTATGSPEMQLFLAIPPEG-IPREELQKKLGPSIFKIGCAQAAKNKWVEMGKQMIS 120
           EG+ Y A GSPE+QLFLA+P EG I ++ELQKKL P++FKIGC+QA KNKWVEMGKQ +S
Sbjct: 61  EGKKYAAEGSPEVQLFLAVPEEGSISKDELQKKLAPAVFKIGCSQAGKNKWVEMGKQ-VS 120

Query: 121 RKVQHVEDKVKNSLLQIKDGKEIYDEDVKALKARKLIVSQTWKGYSVRRGPNYAPRRK-- 180
           RKV+ VEDKVK  LLQI+ GKE   E + +LKARKLIV Q WKGYSV++GPNYAP RK  
Sbjct: 121 RKVKDVEDKVKGQLLQIQQGKEFDKESINSLKARKLIVPQIWKGYSVKKGPNYAPERKKV 180

Query: 181 ------------RELEFKEYNFNAKGQPLLCG--------PTANKNIFRQMGFEEMPTNN 240
                       +ELEFKEYNF AKG P+  G            K+IF QMGFEEMPTNN
Sbjct: 181 ATDLTRENLQNWKELEFKEYNFKAKGAPVDAGHLHALLKVRKQFKDIFVQMGFEEMPTNN 240

Query: 241 YVESSFWNFDALFQPQQHPARDAHDTFFLKAPSTTRELPEDYVERVKQVHETGGYGSKGY 300
           YVESSFWNFDALFQPQQHPARD+HDTFFLK PSTTR LPEDYVERVK+VHE+GGYGS+GY
Sbjct: 241 YVESSFWNFDALFQPQQHPARDSHDTFFLKVPSTTRTLPEDYVERVKRVHESGGYGSRGY 300

Query: 301 GYNWKREEANKNLLRTHTTAVTSRMLYMLAQKPFAPKKYFSIDRVFRNEAVDRTHLAEFH 360
            Y+WKREEANKNLLRTHTTAV+SRMLY LAQKPF PKKYFSIDRVFRNEAVDRTHLAEFH
Sbjct: 301 NYDWKREEANKNLLRTHTTAVSSRMLYALAQKPFVPKKYFSIDRVFRNEAVDRTHLAEFH 360

Query: 361 QIEGLICGRGLTLGDLIGVLYDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGFKKWVE 420
           QIEGLIC RGLTLGDLIGVL DFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEG  KWVE
Sbjct: 361 QIEGLICDRGLTLGDLIGVLEDFFSRLGMSKLRFKPAYNPYTEPSMEIFSYHEGLGKWVE 420

Query: 421 VGNSGMFRPEMLLPMGFPEDARIIAWGLSLERPTMIMYGVDNIRDLFGHKVDLSLVKKNP 464
           +GNSGMFRPEMLLPMG PED R+IAWGLSLERPTMI+YG+DNIRDLFGHKVDL L+K+NP
Sbjct: 421 IGNSGMFRPEMLLPMGLPEDVRVIAWGLSLERPTMILYGIDNIRDLFGHKVDLDLIKRNP 480