PI0007222 (gene) Melon (PI 482460) v1

Overview
NamePI0007222
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionProteasome subunit beta
Locationchr06: 4064106 .. 4066417 (+)
RNA-Seq ExpressionPI0007222
SyntenyPI0007222
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATGATTTAAATCTAAAATTTGAAAAAAAATATAAAAGAGAAAAAAAAAGGGCCAAAGAGAGAACTTGTTCTTCAAGTCGTTGTTGTAAAGCATAATTGTAGAAGGGGAACTCCCAAATTTCACAAAAGGCTTTCAAAACCCAGATCGATTTCAAGAAGAACAACAAGAACAAAGAAGATATGGAGAGCGTATTTGGACTTGTTGGCAACGGATTCGTCATCGTGGCCGCCGACACATCGGCGGTGCACAGTATTTTGGTTCATAAATCCAACGAGGACAAGATCATGGTCCTCGATTCTCACAAGCTCGTCGCAGCTAGCGGCGAACCTGGCGATAGGTTTTTAGATTTCTATTCATACAAAACTTCTTGTTTTTTTTTCTCTCTAATCGTTTGCTTTTAGTCAATTACGTTCTTTCTTTCTGTGATTGAATAGGGTTCAGTTCACGGAGTATATCCAGAAAAATGTGGCTTTGTATCAGTTTCGAAATGGTATCCCTTTAACCACTGCTGCTGCTGCTAACTTCACTCGTGGTGAACTCGCTACCGCCTTACGGAAGGTATATCATTTCTTGTTATTGTAACTTTTATTTGATGATAGTTTCAGCCTTCATTTTTGGGAATTGTGGATCCTGGGCTTTAACTTAGCATTGGAGTGGTTTAAAGCGGTTCTAATCATTCCTTTGTGATGGTAGAATACGAATAGTTTTGTCTTCAATCTAAATTCACTACTTTGTGGAGCTTAGAACTGTTTAGATGTTTATACAGAGCGAGAGAATATTGTGTTGATTTGTTTAGGAAAACCCTTGATTTCTGTCATCTTTAAACATTGCCTCATAATGATAGAAAATTAGACTGATAACAATCTCCGGATGTTGTATCTTAAAACACTTTGTGACTTGTACTTTTGTTCTTGTATTCAGCTGTGTAGAGTGTAGCCAGGCCAAAGTTTGTGTATTTCTCTAATATCATGTTTCGATTTGCTATTGTCATTCGCATGGTTCGTTTCTTACCATTATGGAAGGCGTTTGTTAAATCAGAAAGTTATAATGAACTTACATATTGTTGTCTACTTCTTGCTCCAGCCTCTAGGGATTTCTTATGGATTTTATGCTTTGTAACTTCATTTAATTTCCCTAAATTATGCTAGATATGTGTTCCAATGTAATTTGATGTGTTGGTGGGTGAGAGCGGATTGGTTGTAAAAGAGTAAGGCATACAATGTGGTGACAACTTTATAGAATACTAATACGATTTTCGTGAAAGTTGGTTCTAGTTAACGAAATTATGCTCTATGTTTAGTCAGTTGTGTGCTTGTCAAAGTACCAATTATCATGTTTCACAACTACAAATGAGCTTTTAGCCATAAAAGAGGAGTTTTTATTTGGTTTTTGTTGCTTAGAGCATAATAATAAGTGATCCACTATTCCACTTATAATCCTGCATTTTGATGTATCTATGAATGAACCCTTTGATAAGTCACGAGATTCCTTGCATCATTGCTCTTTGCTGTCATGGGAAATGGAATTATTGGGTAGGCTATAATTTTGAAGGACAGCCTGATTTCAATTGTGAGCTTTTGATTTTCAGAATCCATATTCCGTGAACATTCTTCTGGCGGGATATGACAAGGAGACCGGCCCATCCCTCTATTACATTGACTATATTGCCACCCTTCACAAGGTTGAAAAGGGAGCTTTTGGTTATGGGTCTTACTTTTCACTCGCCATGATGGACAGACACTACCACAGTGGCATGACAGAAGAAGAAGCCATTGATTTGGTTGACAAATGCATACTAGAGATCAGGTCCAGGCTTGTTGTGGCTCCACCGAACTTTGTTATCAAAATTGTAGACAAGAACGGAGCTAGGGAGGTTGCTTGGCGTGAATCCATCAAGGACGGAGTTAGTGTTCCTTCAGCTTAAGATTTAAATGCTATATCAGCAGAGGCTTGCTTTTCTTTCTGTCTTTTTAACTTTGTATGGGTGACGAGCAGGGGTGTATTTTACCCTCGTAGGCGCCCCAGTTTCAGACGTTTTGATAGAGTAATGTTGGGGTTAACGTCTCTTATTCCTGAATACATGAACAACCGAACACCATCTGAACTATAGATTGATTTATCATCTACTGTGCTGAAGTTCAAATGTTCTACTTATGGATAGATTCACAATTAAGTTGGCCTTGCAAGAAATTGTTGTTAGTTTTTTCTTTGTTATGTTGAATCTGAAAAGTGCGATATTTGATAAATCCAGATCTTAACAAACCAGCTTCATTGTCCAAAGAATCTGCTTTCTCATTGCTTGAATTCTTG

mRNA sequence

AAATGATTTAAATCTAAAATTTGAAAAAAAATATAAAAGAGAAAAAAAAAGGGCCAAAGAGAGAACTTGTTCTTCAAGTCGTTGTTGTAAAGCATAATTGTAGAAGGGGAACTCCCAAATTTCACAAAAGGCTTTCAAAACCCAGATCGATTTCAAGAAGAACAACAAGAACAAAGAAGATATGGAGAGCGTATTTGGACTTGTTGGCAACGGATTCGTCATCGTGGCCGCCGACACATCGGCGGTGCACAGTATTTTGGTTCATAAATCCAACGAGGACAAGATCATGGTCCTCGATTCTCACAAGCTCGTCGCAGCTAGCGGCGAACCTGGCGATAGGGTTCAGTTCACGGAGTATATCCAGAAAAATGTGGCTTTGTATCAGTTTCGAAATGGTATCCCTTTAACCACTGCTGCTGCTGCTAACTTCACTCGTGGTGAACTCGCTACCGCCTTACGGAAGAATCCATATTCCGTGAACATTCTTCTGGCGGGATATGACAAGGAGACCGGCCCATCCCTCTATTACATTGACTATATTGCCACCCTTCACAAGGTTGAAAAGGGAGCTTTTGGTTATGGGTCTTACTTTTCACTCGCCATGATGGACAGACACTACCACAGTGGCATGACAGAAGAAGAAGCCATTGATTTGGTTGACAAATGCATACTAGAGATCAGGTCCAGGCTTGTTGTGGCTCCACCGAACTTTGTTATCAAAATTGTAGACAAGAACGGAGCTAGGGAGGTTGCTTGGCGTGAATCCATCAAGGACGGAGTTAGTGTTCCTTCAGCTTAAGATTTAAATGCTATATCAGCAGAGGCTTGCTTTTCTTTCTGTCTTTTTAACTTTGTATGGGTGACGAGCAGGGGTGTATTTTACCCTCGTAGGCGCCCCAGTTTCAGACGTTTTGATAGAGTAATGTTGGGGTTAACGTCTCTTATTCCTGAATACATGAACAACCGAACACCATCTGAACTATAGATTGATTTATCATCTACTGTGCTGAAGTTCAAATGTTCTACTTATGGATAGATTCACAATTAAGTTGGCCTTGCAAGAAATTGTTGTTAGTTTTTTCTTTGTTATGTTGAATCTGAAAAGTGCGATATTTGATAAATCCAGATCTTAACAAACCAGCTTCATTGTCCAAAGAATCTGCTTTCTCATTGCTTGAATTCTTG

Coding sequence (CDS)

ATGGAGAGCGTATTTGGACTTGTTGGCAACGGATTCGTCATCGTGGCCGCCGACACATCGGCGGTGCACAGTATTTTGGTTCATAAATCCAACGAGGACAAGATCATGGTCCTCGATTCTCACAAGCTCGTCGCAGCTAGCGGCGAACCTGGCGATAGGGTTCAGTTCACGGAGTATATCCAGAAAAATGTGGCTTTGTATCAGTTTCGAAATGGTATCCCTTTAACCACTGCTGCTGCTGCTAACTTCACTCGTGGTGAACTCGCTACCGCCTTACGGAAGAATCCATATTCCGTGAACATTCTTCTGGCGGGATATGACAAGGAGACCGGCCCATCCCTCTATTACATTGACTATATTGCCACCCTTCACAAGGTTGAAAAGGGAGCTTTTGGTTATGGGTCTTACTTTTCACTCGCCATGATGGACAGACACTACCACAGTGGCATGACAGAAGAAGAAGCCATTGATTTGGTTGACAAATGCATACTAGAGATCAGGTCCAGGCTTGTTGTGGCTCCACCGAACTTTGTTATCAAAATTGTAGACAAGAACGGAGCTAGGGAGGTTGCTTGGCGTGAATCCATCAAGGACGGAGTTAGTGTTCCTTCAGCTTAA

Protein sequence

MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYIQKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYIATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIKIVDKNGAREVAWRESIKDGVSVPSA
Homology
BLAST of PI0007222 vs. ExPASy Swiss-Prot
Match: O23714 (Proteasome subunit beta type-2-A OS=Arabidopsis thaliana OX=3702 GN=PBD1 PE=1 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 5.2e-97
Identity = 174/198 (87.88%), Postives = 189/198 (95.45%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME VFGLVGNGF IVAADTSAVHSIL+HK+NEDKIMVLDSHKLVAASGEPGDRVQFTEY+
Sbjct: 1   MECVFGLVGNGFAIVAADTSAVHSILLHKNNEDKIMVLDSHKLVAASGEPGDRVQFTEYV 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120
           QKNV+LY+FRNGIPLTTAAAANFTRGELATALRKNPYSVNIL+AGYD E+G SLYYIDYI
Sbjct: 61  QKNVSLYKFRNGIPLTTAAAANFTRGELATALRKNPYSVNILMAGYDDESGASLYYIDYI 120

Query: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKV+KGAFGYGSYFSL+ MDRHY S M+ EEAI+LVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRESIKD 199
           IVDK+GAR+ AWR+S+KD
Sbjct: 181 IVDKDGARDYAWRQSVKD 198

BLAST of PI0007222 vs. ExPASy Swiss-Prot
Match: O24633 (Proteasome subunit beta type-2-B OS=Arabidopsis thaliana OX=3702 GN=PBD2 PE=1 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 1.8e-94
Identity = 171/198 (86.36%), Postives = 183/198 (92.42%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME VFGLVGNGF IVAADTSAVHSIL+HK+ EDKIM LDSHKLVAASGEPGDRVQFTEY+
Sbjct: 1   MECVFGLVGNGFAIVAADTSAVHSILLHKNKEDKIMTLDSHKLVAASGEPGDRVQFTEYV 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120
           QKNV+LYQFRNGIPL+TAAAANFTRGELATALRKNPYSVNIL+AGYDKE G SLYYIDYI
Sbjct: 61  QKNVSLYQFRNGIPLSTAAAANFTRGELATALRKNPYSVNILMAGYDKEAGASLYYIDYI 120

Query: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKV+KGAFGYGSYFSL+ MDRHY S M+ EEAI+LVDKCILEIRSRLV+APPNFVIK
Sbjct: 121 ATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCILEIRSRLVIAPPNFVIK 180

Query: 181 IVDKNGAREVAWRESIKD 199
           IVDK+GARE  WR S  D
Sbjct: 181 IVDKDGAREYGWRISTAD 198

BLAST of PI0007222 vs. ExPASy Swiss-Prot
Match: Q9LST6 (Proteasome subunit beta type-2 OS=Oryza sativa subsp. japonica OX=39947 GN=PBD1 PE=2 SV=2)

HSP 1 Score: 300.8 bits (769), Expect = 1.2e-80
Identity = 148/193 (76.68%), Postives = 166/193 (86.01%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME V G+VG  F +VAADTSAV SILVHK++EDK+MVLDSHKL+ ASGEPGDRVQFTE+I
Sbjct: 1   MECVIGVVGRDFAVVAADTSAVQSILVHKTDEDKVMVLDSHKLMGASGEPGDRVQFTEFI 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120
           QKN+ LYQFRN IPL+TAA ANFTRGELATALRKNPY VN+LLAGYD + G SLYYIDYI
Sbjct: 61  QKNLHLYQFRNNIPLSTAATANFTRGELATALRKNPYYVNVLLAGYDSDVGASLYYIDYI 120

Query: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           AT HK+EKGAFGYGSYF L++MD+ Y   M+ EEA+DLVDKCI EIR RLVVAP NF+IK
Sbjct: 121 ATFHKIEKGAFGYGSYFCLSLMDKLYRPDMSVEEAVDLVDKCIKEIRLRLVVAPQNFIIK 180

Query: 181 IVDKNGAREVAWR 194
           IVDK GARE A R
Sbjct: 181 IVDKEGAREYARR 193

BLAST of PI0007222 vs. ExPASy Swiss-Prot
Match: Q55DY7 (Proteasome subunit beta type-2 OS=Dictyostelium discoideum OX=44689 GN=psmB2 PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 4.2e-46
Identity = 90/191 (47.12%), Postives = 130/191 (68.06%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME++      G V+  AD+S  HSI+  K +EDKI+ +D HKL+ ++GE GDRVQFTEYI
Sbjct: 1   METLLAFKVKGGVLCLADSSVNHSIVKMKDDEDKILEIDGHKLLLSAGEAGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKET---GPSLYYI 120
            KN+ LY  RN  P++T AAANF R ELAT++R +PYS+N++LAGYDKE    G SLYY+
Sbjct: 61  SKNIKLYSLRNSYPMSTPAAANFIRNELATSIRSHPYSINLILAGYDKEVEDGGTSLYYM 120

Query: 121 DYIATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNF 180
           DY+ +L K+  G  GY SYF L ++DRH+   ++ E+ I+L+  C  E+++R +V+   +
Sbjct: 121 DYLGSLQKLNFGCHGYASYFLLGLLDRHHKCDLSLEDGINLMHLCTTELKTRFLVS-GKY 180

Query: 181 VIKIVDKNGAR 189
            +K V   G +
Sbjct: 181 TLKFVSSEGIK 190

BLAST of PI0007222 vs. ExPASy Swiss-Prot
Match: P49721 (Proteasome subunit beta type-2 OS=Homo sapiens OX=9606 GN=PSMB2 PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 3.0e-44
Identity = 90/204 (44.12%), Postives = 132/204 (64.71%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME + G+ G  +V+VA+D  A  +I+  K + DK+  +    L+   GE GD VQF EYI
Sbjct: 1   MEYLIGIQGPDYVLVASDRVAASNIVQMKDDHDKMFKMSEKILLLCVGEAGDTVQFAEYI 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALR-KNPYSVNILLAGYDKETGPSLYYIDY 120
           QKNV LY+ RNG  L+  AAANFTR  LA  LR + PY VN+LLAGYD+  GP+LYY+DY
Sbjct: 61  QKNVQLYKMRNGYELSPTAAANFTRRNLADCLRSRTPYHVNLLLAGYDEHEGPALYYMDY 120

Query: 121 IATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVI 180
           +A L K    A GYG++ +L+++DR+Y   ++ E A++L+ KC+ E++ R ++  P F +
Sbjct: 121 LAALAKAPFAAHGYGAFLTLSILDRYYTPTISRERAVELLRKCLEELQKRFILNLPTFSV 180

Query: 181 KIVDKNGAREVAWRESIKDGVSVP 204
           +I+DKNG  ++       D +S P
Sbjct: 181 RIIDKNGIHDL-------DNISFP 197

BLAST of PI0007222 vs. ExPASy TrEMBL
Match: A0A5D3D072 (Proteasome subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00590 PE=3 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 5.5e-110
Identity = 204/205 (99.51%), Postives = 205/205 (100.00%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120
           QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVN+LLAGYDKETGPSLYYIDYI
Sbjct: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNVLLAGYDKETGPSLYYIDYI 120

Query: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRESIKDGVSVPSA 206
           IVDKNGAREVAWRESIKDGVSVPSA
Sbjct: 181 IVDKNGAREVAWRESIKDGVSVPSA 205

BLAST of PI0007222 vs. ExPASy TrEMBL
Match: A0A1S3AXK5 (Proteasome subunit beta OS=Cucumis melo OX=3656 GN=LOC103483693 PE=3 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 5.5e-110
Identity = 204/205 (99.51%), Postives = 205/205 (100.00%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120
           QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVN+LLAGYDKETGPSLYYIDYI
Sbjct: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNVLLAGYDKETGPSLYYIDYI 120

Query: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRESIKDGVSVPSA 206
           IVDKNGAREVAWRESIKDGVSVPSA
Sbjct: 181 IVDKNGAREVAWRESIKDGVSVPSA 205

BLAST of PI0007222 vs. ExPASy TrEMBL
Match: A0A0A0LA70 (Proteasome subunit beta OS=Cucumis sativus OX=3659 GN=Csa_3G146470 PE=3 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 2.7e-109
Identity = 203/205 (99.02%), Postives = 204/205 (99.51%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120
           QKNVALYQFRNGIPLTTAAAANFTR ELATALRKNPYSVNILLAGYDKETGPSLYY+DYI
Sbjct: 61  QKNVALYQFRNGIPLTTAAAANFTRSELATALRKNPYSVNILLAGYDKETGPSLYYVDYI 120

Query: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRESIKDGVSVPSA 206
           IVDKNGAREVAWRESIKDGVSVPSA
Sbjct: 181 IVDKNGAREVAWRESIKDGVSVPSA 205

BLAST of PI0007222 vs. ExPASy TrEMBL
Match: A0A6J1F7I3 (Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111441567 PE=3 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.3e-108
Identity = 201/205 (98.05%), Postives = 203/205 (99.02%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120
           QKNV+LYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI
Sbjct: 61  QKNVSLYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120

Query: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTE+EAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEQEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRESIKDGVSVPSA 206
           IVDKNGAREVAWRESIKDG S PSA
Sbjct: 181 IVDKNGAREVAWRESIKDGASFPSA 205

BLAST of PI0007222 vs. ExPASy TrEMBL
Match: A0A6J1IJB4 (Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111474859 PE=3 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 6.8e-108
Identity = 199/205 (97.07%), Postives = 203/205 (99.02%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120
           QKNV+LYQFRNGIPLTTAAAAN+TRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI
Sbjct: 61  QKNVSLYQFRNGIPLTTAAAANYTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120

Query: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTE+EAIDL+DKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEQEAIDLIDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRESIKDGVSVPSA 206
           IVDKNGAREVAWRESIKDG S PSA
Sbjct: 181 IVDKNGAREVAWRESIKDGASFPSA 205

BLAST of PI0007222 vs. NCBI nr
Match: XP_008438650.1 (PREDICTED: proteasome subunit beta type-2-A [Cucumis melo] >KAA0049346.1 proteasome subunit beta type-2-A [Cucumis melo var. makuwa] >TYK17212.1 proteasome subunit beta type-2-A [Cucumis melo var. makuwa])

HSP 1 Score: 406.8 bits (1044), Expect = 1.1e-109
Identity = 204/205 (99.51%), Postives = 205/205 (100.00%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120
           QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVN+LLAGYDKETGPSLYYIDYI
Sbjct: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNVLLAGYDKETGPSLYYIDYI 120

Query: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRESIKDGVSVPSA 206
           IVDKNGAREVAWRESIKDGVSVPSA
Sbjct: 181 IVDKNGAREVAWRESIKDGVSVPSA 205

BLAST of PI0007222 vs. NCBI nr
Match: XP_038875710.1 (proteasome subunit beta type-2-A-like [Benincasa hispida])

HSP 1 Score: 406.0 bits (1042), Expect = 1.9e-109
Identity = 204/205 (99.51%), Postives = 205/205 (100.00%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120
           QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI
Sbjct: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120

Query: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKV+KGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVDKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRESIKDGVSVPSA 206
           IVDKNGAREVAWRESIKDGVSVPSA
Sbjct: 181 IVDKNGAREVAWRESIKDGVSVPSA 205

BLAST of PI0007222 vs. NCBI nr
Match: XP_011650969.1 (proteasome subunit beta type-2-A [Cucumis sativus] >KGN56961.1 hypothetical protein Csa_011179 [Cucumis sativus])

HSP 1 Score: 404.4 bits (1038), Expect = 5.7e-109
Identity = 203/205 (99.02%), Postives = 204/205 (99.51%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120
           QKNVALYQFRNGIPLTTAAAANFTR ELATALRKNPYSVNILLAGYDKETGPSLYY+DYI
Sbjct: 61  QKNVALYQFRNGIPLTTAAAANFTRSELATALRKNPYSVNILLAGYDKETGPSLYYVDYI 120

Query: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRESIKDGVSVPSA 206
           IVDKNGAREVAWRESIKDGVSVPSA
Sbjct: 181 IVDKNGAREVAWRESIKDGVSVPSA 205

BLAST of PI0007222 vs. NCBI nr
Match: XP_022934380.1 (proteasome subunit beta type-2-A-like [Cucurbita moschata])

HSP 1 Score: 401.4 bits (1030), Expect = 4.8e-108
Identity = 201/205 (98.05%), Postives = 203/205 (99.02%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120
           QKNV+LYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI
Sbjct: 61  QKNVSLYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120

Query: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTE+EAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEQEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRESIKDGVSVPSA 206
           IVDKNGAREVAWRESIKDG S PSA
Sbjct: 181 IVDKNGAREVAWRESIKDGASFPSA 205

BLAST of PI0007222 vs. NCBI nr
Match: XP_023539479.1 (proteasome subunit beta type-2-A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 400.2 bits (1027), Expect = 1.1e-107
Identity = 200/205 (97.56%), Postives = 203/205 (99.02%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI
Sbjct: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120
           QKNV+LYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDK+TGPSLYYIDYI
Sbjct: 61  QKNVSLYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKQTGPSLYYIDYI 120

Query: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTE+EAIDLVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEQEAIDLVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRESIKDGVSVPSA 206
           IVDKNGAREVAWRESIKDG S PSA
Sbjct: 181 IVDKNGAREVAWRESIKDGASFPSA 205

BLAST of PI0007222 vs. TAIR 10
Match: AT3G22630.1 (20S proteasome beta subunit D1 )

HSP 1 Score: 355.1 bits (910), Expect = 3.7e-98
Identity = 174/198 (87.88%), Postives = 189/198 (95.45%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME VFGLVGNGF IVAADTSAVHSIL+HK+NEDKIMVLDSHKLVAASGEPGDRVQFTEY+
Sbjct: 1   MECVFGLVGNGFAIVAADTSAVHSILLHKNNEDKIMVLDSHKLVAASGEPGDRVQFTEYV 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120
           QKNV+LY+FRNGIPLTTAAAANFTRGELATALRKNPYSVNIL+AGYD E+G SLYYIDYI
Sbjct: 61  QKNVSLYKFRNGIPLTTAAAANFTRGELATALRKNPYSVNILMAGYDDESGASLYYIDYI 120

Query: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKV+KGAFGYGSYFSL+ MDRHY S M+ EEAI+LVDKCILEIRSRLVVAPPNFVIK
Sbjct: 121 ATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCILEIRSRLVVAPPNFVIK 180

Query: 181 IVDKNGAREVAWRESIKD 199
           IVDK+GAR+ AWR+S+KD
Sbjct: 181 IVDKDGARDYAWRQSVKD 198

BLAST of PI0007222 vs. TAIR 10
Match: AT4G14800.1 (20S proteasome beta subunit D2 )

HSP 1 Score: 346.7 bits (888), Expect = 1.3e-95
Identity = 171/198 (86.36%), Postives = 183/198 (92.42%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME VFGLVGNGF IVAADTSAVHSIL+HK+ EDKIM LDSHKLVAASGEPGDRVQFTEY+
Sbjct: 1   MECVFGLVGNGFAIVAADTSAVHSILLHKNKEDKIMTLDSHKLVAASGEPGDRVQFTEYV 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRKNPYSVNILLAGYDKETGPSLYYIDYI 120
           QKNV+LYQFRNGIPL+TAAAANFTRGELATALRKNPYSVNIL+AGYDKE G SLYYIDYI
Sbjct: 61  QKNVSLYQFRNGIPLSTAAAANFTRGELATALRKNPYSVNILMAGYDKEAGASLYYIDYI 120

Query: 121 ATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCILEIRSRLVVAPPNFVIK 180
           ATLHKV+KGAFGYGSYFSL+ MDRHY S M+ EEAI+LVDKCILEIRSRLV+APPNFVIK
Sbjct: 121 ATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCILEIRSRLVIAPPNFVIK 180

Query: 181 IVDKNGAREVAWRESIKD 199
           IVDK+GARE  WR S  D
Sbjct: 181 IVDKDGAREYGWRISTAD 198

BLAST of PI0007222 vs. TAIR 10
Match: AT4G14800.2 (20S proteasome beta subunit D2 )

HSP 1 Score: 336.3 bits (861), Expect = 1.8e-92
Identity = 171/214 (79.91%), Postives = 183/214 (85.51%), Query Frame = 0

Query: 1   MESVFGLVGNGFVIVAADTSAVHSILVHKSNEDKIMVLDSHKLVAASGEPGDRVQFTEYI 60
           ME VFGLVGNGF IVAADTSAVHSIL+HK+ EDKIM LDSHKLVAASGEPGDRVQFTEY+
Sbjct: 1   MECVFGLVGNGFAIVAADTSAVHSILLHKNKEDKIMTLDSHKLVAASGEPGDRVQFTEYV 60

Query: 61  QKNVALYQFRNGIPLTTAAAANFTRGELATALRK----------------NPYSVNILLA 120
           QKNV+LYQFRNGIPL+TAAAANFTRGELATALRK                NPYSVNIL+A
Sbjct: 61  QKNVSLYQFRNGIPLSTAAAANFTRGELATALRKEFCATFQGYSDFCVLMNPYSVNILMA 120

Query: 121 GYDKETGPSLYYIDYIATLHKVEKGAFGYGSYFSLAMMDRHYHSGMTEEEAIDLVDKCIL 180
           GYDKE G SLYYIDYIATLHKV+KGAFGYGSYFSL+ MDRHY S M+ EEAI+LVDKCIL
Sbjct: 121 GYDKEAGASLYYIDYIATLHKVDKGAFGYGSYFSLSTMDRHYRSDMSVEEAIELVDKCIL 180

Query: 181 EIRSRLVVAPPNFVIKIVDKNGAREVAWRESIKD 199
           EIRSRLV+APPNFVIKIVDK+GARE  WR S  D
Sbjct: 181 EIRSRLVIAPPNFVIKIVDKDGAREYGWRISTAD 214

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O237145.2e-9787.88Proteasome subunit beta type-2-A OS=Arabidopsis thaliana OX=3702 GN=PBD1 PE=1 SV... [more]
O246331.8e-9486.36Proteasome subunit beta type-2-B OS=Arabidopsis thaliana OX=3702 GN=PBD2 PE=1 SV... [more]
Q9LST61.2e-8076.68Proteasome subunit beta type-2 OS=Oryza sativa subsp. japonica OX=39947 GN=PBD1 ... [more]
Q55DY74.2e-4647.12Proteasome subunit beta type-2 OS=Dictyostelium discoideum OX=44689 GN=psmB2 PE=... [more]
P497213.0e-4444.12Proteasome subunit beta type-2 OS=Homo sapiens OX=9606 GN=PSMB2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3D0725.5e-11099.51Proteasome subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3AXK55.5e-11099.51Proteasome subunit beta OS=Cucumis melo OX=3656 GN=LOC103483693 PE=3 SV=1[more]
A0A0A0LA702.7e-10999.02Proteasome subunit beta OS=Cucumis sativus OX=3659 GN=Csa_3G146470 PE=3 SV=1[more]
A0A6J1F7I32.3e-10898.05Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111441567 PE=3 SV=1[more]
A0A6J1IJB46.8e-10897.07Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111474859 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008438650.11.1e-10999.51PREDICTED: proteasome subunit beta type-2-A [Cucumis melo] >KAA0049346.1 proteas... [more]
XP_038875710.11.9e-10999.51proteasome subunit beta type-2-A-like [Benincasa hispida][more]
XP_011650969.15.7e-10999.02proteasome subunit beta type-2-A [Cucumis sativus] >KGN56961.1 hypothetical prot... [more]
XP_022934380.14.8e-10898.05proteasome subunit beta type-2-A-like [Cucurbita moschata][more]
XP_023539479.11.1e-10797.56proteasome subunit beta type-2-A-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G22630.13.7e-9887.8820S proteasome beta subunit D1 [more]
AT4G14800.11.3e-9586.3620S proteasome beta subunit D2 [more]
AT4G14800.21.8e-9279.9120S proteasome beta subunit D2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001353Proteasome, subunit alpha/betaPFAMPF00227Proteasomecoord: 3..182
e-value: 3.7E-41
score: 140.7
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 1..192
e-value: 2.9E-54
score: 185.7
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 1..190
NoneNo IPR availablePANTHERPTHR11599:SF181PROTEASOME SUBUNIT BETA TYPE-2-Bcoord: 1..199
NoneNo IPR availablePANTHERPTHR11599PROTEASOME SUBUNIT ALPHA/BETAcoord: 1..199
IPR016050Proteasome beta-type subunit, conserved sitePROSITEPS00854PROTEASOME_BETA_1coord: 5..52
IPR023333Proteasome B-type subunitPROSITEPS51476PROTEASOME_BETA_2coord: 1..182
score: 42.486168
IPR035206Proteasome subunit beta 2CDDcd03758proteasome_beta_type_2coord: 1..192
e-value: 2.61034E-116
score: 326.85

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0007222.1PI0007222.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0010498 proteasomal protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
cellular_component GO:0019774 proteasome core complex, beta-subunit complex
cellular_component GO:0005839 proteasome core complex
molecular_function GO:0004298 threonine-type endopeptidase activity