PI0007006 (gene) Melon (PI 482460) v1

Overview
NamePI0007006
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr01: 14659488 .. 14660381 (+)
RNA-Seq ExpressionPI0007006
SyntenyPI0007006
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCATTATAGGCCTATTTCGTGTTGTAATGTGGTTTACAAGTGCATTTCTAAGATTTTGGCGGATAGGCTTCGTGTGTGGCTTCCTTCTTTGATCAGTGGTAATCAGTCAGCCTTTGTTGTTGGGAGGAGTATTGTTGATAACATTCTGCTTTGTCAGGAGATGGTGGGGGATTATCATGGTTCTTCGGGTCCGCCTAGGTGTGCGATGAAGGTGGATCTTCAAAAGGCTTATGATTCGGTGAATTGGGATTTTCTGTTCAGCTTGTTGCTTACTATTGGCACTCCCTTGCGTTTTGTGAGTTGGATTAGGGCGTGTGTTACTTCTCCTATGTTCTCTATCATGGTTAATGGTTCTGTCGAGGGATTTTTTCCTGGTAGAAAAGGGTTGCGGCAGGGTGAACCTTTATCCCCCTTTTTGTTTGTGATGGTGATGGAGGTGCTAACTCGTCTTTTGAGAAGGCCCTCTCTGGATTTCAAGTATCATCATCGTTGTGAGAAGGTTTGTTTGACGCATCTAACCTTCGCTGATGATCTTATGATTTTTTGTTATGCTGACACCGCTTCCATTGGCTTTGTGTGCGAGACTCTTCGTTAGTTTGGGGAGTTATCGGGGTTAATTGCTAATCTGGAAAAGAGCTCTATGTTTATGGCGGGGGTTGACATTGAGGTTGCTACTGTGTTGGCTGATAGTATGGGGTTTGCGCTGGGTACTTTGCCTGTTCGTTACCTGGGTGTTCCCTTACTCTCTGGTCGTCTGCGATCTTCGAATTGTGCTCCGCTCATCCAGCGTATTACTAGTCGAATTCGGTCTTGGTCGGCTAGAGTATTATCCTTCGCTGGTAGACTTCAGCTTGTGCGTTCGGTGTTGCGGAGTTTTCAGGTCTTTTAG

mRNA sequence

ATGGAGCATTATAGGCCTATTTCGTGTTGTAATGTGGTTTACAAGTGCATTTCTAAGATTTTGGCGGATAGGCTTCGTGTGTGGCTTCCTTCTTTGATCAGTGGTAATCAGTCAGCCTTTGTTGTTGGGAGGAGTATTGTTGATAACATTCTGCTTTGTCAGGAGATGGTGGGGGATTATCATGGTTCTTCGGGTCCGCCTAGGTGTGCGATGAAGTTATCGGGGTTAATTGCTAATCTGGAAAAGAGCTCTATGTTTATGGCGGGGGTTGACATTGAGGTTGCTACTGTGTTGGCTGATAGTATGGGGTTTGCGCTGGGTACTTTGCCTGTTCGTTACCTGGGTGTTCCCTTACTCTCTGGTCGTCTGCGATCTTCGAATTGTGCTCCGCTCATCCAGCGTATTACTAGTCGAATTCGGTCTTGGTCGGCTAGAGTATTATCCTTCGCTGGTAGACTTCAGCTTGTGCGTTCGGTGTTGCGGAGTTTTCAGGTCTTTTAG

Coding sequence (CDS)

ATGGAGCATTATAGGCCTATTTCGTGTTGTAATGTGGTTTACAAGTGCATTTCTAAGATTTTGGCGGATAGGCTTCGTGTGTGGCTTCCTTCTTTGATCAGTGGTAATCAGTCAGCCTTTGTTGTTGGGAGGAGTATTGTTGATAACATTCTGCTTTGTCAGGAGATGGTGGGGGATTATCATGGTTCTTCGGGTCCGCCTAGGTGTGCGATGAAGTTATCGGGGTTAATTGCTAATCTGGAAAAGAGCTCTATGTTTATGGCGGGGGTTGACATTGAGGTTGCTACTGTGTTGGCTGATAGTATGGGGTTTGCGCTGGGTACTTTGCCTGTTCGTTACCTGGGTGTTCCCTTACTCTCTGGTCGTCTGCGATCTTCGAATTGTGCTCCGCTCATCCAGCGTATTACTAGTCGAATTCGGTCTTGGTCGGCTAGAGTATTATCCTTCGCTGGTAGACTTCAGCTTGTGCGTTCGGTGTTGCGGAGTTTTCAGGTCTTTTAG

Protein sequence

MEHYRPISCCNVVYKCISKILADRLRVWLPSLISGNQSAFVVGRSIVDNILLCQEMVGDYHGSSGPPRCAMKLSGLIANLEKSSMFMAGVDIEVATVLADSMGFALGTLPVRYLGVPLLSGRLRSSNCAPLIQRITSRIRSWSARVLSFAGRLQLVRSVLRSFQVF
Homology
BLAST of PI0007006 vs. ExPASy TrEMBL
Match: A0A5A7V4C1 (Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G00110 PE=4 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 3.4e-57
Identity = 123/195 (63.08%), Postives = 141/195 (72.31%), Query Frame = 0

Query: 1   MEHYRPISCCNVVYKCISKILADRLRVWLPSLISGNQSAFVVGRSIVDNILLCQEMVGDY 60
           ME +RPISCCNV+YKCISKILADR+ VWLPS IS NQSAF+ GRSI+DNILLCQE+VG Y
Sbjct: 197 MEEFRPISCCNVIYKCISKILADRICVWLPSFISSNQSAFMPGRSIIDNILLCQELVGGY 256

Query: 61  HGSSGPPRCAMKL-----------------------------SGLIANLEKSSMFMAGVD 120
           H + G PR  +K+                             SGL ANL KSS+F+AGV 
Sbjct: 257 HLNFGKPRYTLKVDLQKAYDSVNWDFLFGLLIAIGTPLKFVTSGLFANLRKSSIFVAGVS 316

Query: 121 IEVATVLADSMGFALGTLPVRYLGVPLLSGRLRSSNCAPLIQRITSRIRSWSARVLSFAG 167
            E A+ LA SMGF LG LPVRYLG+PLL+GRLRS++CAPLIQRITS IRSW+ARVLSF G
Sbjct: 317 HEAASHLAASMGFGLGNLPVRYLGLPLLTGRLRSNDCAPLIQRITSWIRSWTARVLSFFG 376

BLAST of PI0007006 vs. ExPASy TrEMBL
Match: A0A1S4E258 (uncharacterized protein LOC103496880 OS=Cucumis melo OX=3656 GN=LOC103496880 PE=4 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.6e-54
Identity = 120/191 (62.83%), Postives = 138/191 (72.25%), Query Frame = 0

Query: 1   MEHYRPISCCNVVYKCISKILADRLRVWLPSLISGNQSAFVVGRSIVDNILLCQEMVGDY 60
           ME +RPISCCNV+YKCISKILADR+ VWLPS IS NQSAF+ GRSI+DNILLCQE+VG Y
Sbjct: 197 MEEFRPISCCNVIYKCISKILADRICVWLPSFISSNQSAFMPGRSIIDNILLCQELVGGY 256

Query: 61  HGSSGPPRCAMK--------------LSGLI-----------ANLEKSSMFMAGVDIEVA 120
           H + G PR  +K              L GL+               KSS+F+AGV  E A
Sbjct: 257 HLNFGKPRYTLKVDLQKAYDSVNWDFLFGLLIAIGTPLKFERCKARKSSIFVAGVSHEAA 316

Query: 121 TVLADSMGFALGTLPVRYLGVPLLSGRLRSSNCAPLIQRITSRIRSWSARVLSFAGRLQL 167
           + LA SMGF LG LPVRYLG+PLL+GRLRS++CAPLIQRITS IRSW+ARVLSF G LQL
Sbjct: 317 SHLAASMGFGLGNLPVRYLGLPLLTGRLRSNDCAPLIQRITSWIRSWTARVLSFFGTLQL 376

BLAST of PI0007006 vs. ExPASy TrEMBL
Match: A0A1S3BSI8 (uncharacterized protein LOC103493225 OS=Cucumis melo OX=3656 GN=LOC103493225 PE=4 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 3.3e-52
Identity = 124/237 (52.32%), Postives = 143/237 (60.34%), Query Frame = 0

Query: 1   MEHYRPISCCNVVYKCISKILADRLRVWLPSLISGNQSAFVVGRSIVDNILLCQEMVGDY 60
           +E +RPISCCNV+YKCISKILADRLRVWLPS IS NQSAF++GRSI++NILLCQE+VG Y
Sbjct: 399 LEDFRPISCCNVLYKCISKILADRLRVWLPSFISSNQSAFILGRSIIENILLCQELVGGY 458

Query: 61  HGSSGPPRCAMK------------------------------------------------ 120
           H +SG PRC +K                                                
Sbjct: 459 HLNSGKPRCTLKVDLQKAYDSVNWDFLFGLQISISTPLKKGVRQGDPLSLFLFVMVMEVL 518

Query: 121 -----------------------LSGLIANLEKSSMFMAGVDIEVATVLADSMGFALGTL 167
                                  LSGL AN  KSS+F+AGV+ E A+ LA  MGF  G L
Sbjct: 519 SRMLNKIPQSFQFHHRCEKRFGELSGLFANPRKSSIFIAGVNNENASRLAACMGFVRGNL 578

BLAST of PI0007006 vs. ExPASy TrEMBL
Match: A0A5A7V5J2 (Non-LTR retroelement reverse transcriptase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold387G00210 PE=4 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 1.0e-50
Identity = 119/226 (52.65%), Postives = 138/226 (61.06%), Query Frame = 0

Query: 1   MEHYRPISCCNVVYKCISKILADRLRVWLPSLISGNQSAFVVGRSIVDNILLCQEMVGDY 60
           ME +RPISCCNV+YKCISKILADRLRVWLPS I  NQSAF+ GRSI+DNILLCQE+V  Y
Sbjct: 753 MEEFRPISCCNVIYKCISKILADRLRVWLPSFIGSNQSAFIPGRSIIDNILLCQELVEGY 812

Query: 61  HGSSGPPRCAMK-----------------------------------LSGLIANLEKSSM 120
           H +SG PRC +K                                   LS ++  + +S  
Sbjct: 813 HLNSGKPRCTLKVDLQKAYDSVNWDFLKGVRQGDPLSPFLFVMVMEVLSRMLNKIPQSFK 872

Query: 121 F-------------------------MAGVDIEVATVLADSMGFALGTLPVRYLGVPLLS 167
           F                            +  E A+ LA SMGF LG LPVRYLG+PLL+
Sbjct: 873 FHHRCEKVKLTHLTFADDLMIFCAADEPSLSNEAASHLAASMGFVLGNLPVRYLGLPLLT 932

BLAST of PI0007006 vs. ExPASy TrEMBL
Match: A0A5A7UP65 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43054G00070 PE=4 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 7.5e-49
Identity = 124/266 (46.62%), Postives = 143/266 (53.76%), Query Frame = 0

Query: 1   MEHYRPISCCNVVYKCISKILADRLRVWLPSLISGNQSAFVVGRSIVDNILLCQEMVGDY 60
           +E +RPISCCNV+YKCISKILADRLRVWLPS IS NQSAF++GRSI++NILLCQE+VG Y
Sbjct: 436 LEDFRPISCCNVLYKCISKILADRLRVWLPSFISSNQSAFILGRSIIENILLCQELVGGY 495

Query: 61  HGSSGPPRCAMK------------------------------------------------ 120
           H +SG PRC +K                                                
Sbjct: 496 HLNSGKPRCTLKVDLQKAYDSVNWDFLFGLQISISTPLKFVSWISACVTSPMFSIMINGS 555

Query: 121 ----------------------------------------------------LSGLIANL 167
                                                               LSGL AN 
Sbjct: 556 LEGFFHGRKGVRQGDPLSLFLFVMVMEVLSRMLNKIPQSFQFHHRCEKRFGELSGLFANP 615

BLAST of PI0007006 vs. NCBI nr
Match: KAA0060725.1 (uncharacterized protein E6C27_scaffold72G00120 [Cucumis melo var. makuwa] >TYK01467.1 uncharacterized protein E5676_scaffold451G00110 [Cucumis melo var. makuwa])

HSP 1 Score: 231.1 bits (588), Expect = 7.0e-57
Identity = 123/195 (63.08%), Postives = 141/195 (72.31%), Query Frame = 0

Query: 1   MEHYRPISCCNVVYKCISKILADRLRVWLPSLISGNQSAFVVGRSIVDNILLCQEMVGDY 60
           ME +RPISCCNV+YKCISKILADR+ VWLPS IS NQSAF+ GRSI+DNILLCQE+VG Y
Sbjct: 197 MEEFRPISCCNVIYKCISKILADRICVWLPSFISSNQSAFMPGRSIIDNILLCQELVGGY 256

Query: 61  HGSSGPPRCAMKL-----------------------------SGLIANLEKSSMFMAGVD 120
           H + G PR  +K+                             SGL ANL KSS+F+AGV 
Sbjct: 257 HLNFGKPRYTLKVDLQKAYDSVNWDFLFGLLIAIGTPLKFVTSGLFANLRKSSIFVAGVS 316

Query: 121 IEVATVLADSMGFALGTLPVRYLGVPLLSGRLRSSNCAPLIQRITSRIRSWSARVLSFAG 167
            E A+ LA SMGF LG LPVRYLG+PLL+GRLRS++CAPLIQRITS IRSW+ARVLSF G
Sbjct: 317 HEAASHLAASMGFGLGNLPVRYLGLPLLTGRLRSNDCAPLIQRITSWIRSWTARVLSFFG 376

BLAST of PI0007006 vs. NCBI nr
Match: XP_031740402.1 (uncharacterized protein LOC116403409 [Cucumis sativus] >XP_031740413.1 uncharacterized protein LOC116403417 [Cucumis sativus] >XP_031745405.1 uncharacterized protein LOC116405607 [Cucumis sativus] >XP_031745422.1 uncharacterized protein LOC116405638 [Cucumis sativus] >XP_031745640.1 uncharacterized protein LOC116406072 [Cucumis sativus])

HSP 1 Score: 226.9 bits (577), Expect = 1.3e-55
Identity = 123/214 (57.48%), Postives = 142/214 (66.36%), Query Frame = 0

Query: 1   MEHYRPISCCNVVYKCISKILADRLRVWLPSLISGNQSAFVVGRSIVDNILLCQEMVGDY 60
           +E + PISCC+V+YKCIS+ILADRLRVWLPS +SGNQ AF+ GRSI+DNILLCQE+VG Y
Sbjct: 302 LEDFSPISCCSVIYKCISRILADRLRVWLPSFVSGNQPAFIPGRSIIDNILLCQELVGAY 361

Query: 61  HGSSGPPRCAMK------------------------------------------------ 120
           H   G PRC MK                                                
Sbjct: 362 HLHRGKPRCTMKVDLQKAYDYVNWDFLFGLLIAIDDLMIFCTADNHSMSFIKETIKRFGE 421

Query: 121 LSGLIANLEKSSMFMAGVDIEVATVLADSMGFALGTLPVRYLGVPLLSGRLRSSNCAPLI 167
           LSGL ANL KS +F+ GV+   A+ LA +MGF++G LPVRYLG+PLLS RLRSS+C PLI
Sbjct: 422 LSGLFANLAKSLIFLVGVNSSKASRLAANMGFSIGHLPVRYLGLPLLSRRLRSSDCDPLI 481

BLAST of PI0007006 vs. NCBI nr
Match: XP_016902060.1 (PREDICTED: uncharacterized protein LOC103496880 [Cucumis melo])

HSP 1 Score: 222.2 bits (565), Expect = 3.2e-54
Identity = 120/191 (62.83%), Postives = 138/191 (72.25%), Query Frame = 0

Query: 1   MEHYRPISCCNVVYKCISKILADRLRVWLPSLISGNQSAFVVGRSIVDNILLCQEMVGDY 60
           ME +RPISCCNV+YKCISKILADR+ VWLPS IS NQSAF+ GRSI+DNILLCQE+VG Y
Sbjct: 197 MEEFRPISCCNVIYKCISKILADRICVWLPSFISSNQSAFMPGRSIIDNILLCQELVGGY 256

Query: 61  HGSSGPPRCAMK--------------LSGLI-----------ANLEKSSMFMAGVDIEVA 120
           H + G PR  +K              L GL+               KSS+F+AGV  E A
Sbjct: 257 HLNFGKPRYTLKVDLQKAYDSVNWDFLFGLLIAIGTPLKFERCKARKSSIFVAGVSHEAA 316

Query: 121 TVLADSMGFALGTLPVRYLGVPLLSGRLRSSNCAPLIQRITSRIRSWSARVLSFAGRLQL 167
           + LA SMGF LG LPVRYLG+PLL+GRLRS++CAPLIQRITS IRSW+ARVLSF G LQL
Sbjct: 317 SHLAASMGFGLGNLPVRYLGLPLLTGRLRSNDCAPLIQRITSWIRSWTARVLSFFGTLQL 376

BLAST of PI0007006 vs. NCBI nr
Match: XP_031745634.1 (uncharacterized protein LOC116406053 [Cucumis sativus])

HSP 1 Score: 220.3 bits (560), Expect = 1.2e-53
Identity = 115/174 (66.09%), Postives = 135/174 (77.59%), Query Frame = 0

Query: 1   MEHYRPISCCNVVYKCISKILADRLRVWLPSLISGNQSAFVVGRSIVDNILLCQEMVGDY 60
           +E + PISCC+V+YKCIS+ILADRLRVWLPS +SGNQ AF+ GRSI+DNILLCQE+VG Y
Sbjct: 639 LEDFSPISCCSVIYKCISRILADRLRVWLPSFVSGNQPAFIPGRSIIDNILLCQELVGAY 698

Query: 61  HGSSGPPRCAMKLSGLIANLEKSSMFMAGVDIEVATV--------LADSMGFALGTLPVR 120
           H   G PRC MK+    A    +  F+ G+ I + T         LA +MGF++G LPVR
Sbjct: 699 HLHRGKPRCTMKVDLQKAYDYVNWDFLFGLLIAIGTPLRFFESSRLAANMGFSIGHLPVR 758

Query: 121 YLGVPLLSGRLRSSNCAPLIQRITSRIRSWSARVLSFAGRLQLVRSVLRSFQVF 167
           YLG+PLLS RLRSS+C PLIQRITSRIRSWSARVLSFAGRLQLVRSVLRS QV+
Sbjct: 759 YLGLPLLSRRLRSSDCDPLIQRITSRIRSWSARVLSFAGRLQLVRSVLRSLQVY 812

BLAST of PI0007006 vs. NCBI nr
Match: XP_008452126.1 (PREDICTED: uncharacterized protein LOC103493225 [Cucumis melo])

HSP 1 Score: 214.5 bits (545), Expect = 6.7e-52
Identity = 124/237 (52.32%), Postives = 143/237 (60.34%), Query Frame = 0

Query: 1   MEHYRPISCCNVVYKCISKILADRLRVWLPSLISGNQSAFVVGRSIVDNILLCQEMVGDY 60
           +E +RPISCCNV+YKCISKILADRLRVWLPS IS NQSAF++GRSI++NILLCQE+VG Y
Sbjct: 399 LEDFRPISCCNVLYKCISKILADRLRVWLPSFISSNQSAFILGRSIIENILLCQELVGGY 458

Query: 61  HGSSGPPRCAMK------------------------------------------------ 120
           H +SG PRC +K                                                
Sbjct: 459 HLNSGKPRCTLKVDLQKAYDSVNWDFLFGLQISISTPLKKGVRQGDPLSLFLFVMVMEVL 518

Query: 121 -----------------------LSGLIANLEKSSMFMAGVDIEVATVLADSMGFALGTL 167
                                  LSGL AN  KSS+F+AGV+ E A+ LA  MGF  G L
Sbjct: 519 SRMLNKIPQSFQFHHRCEKRFGELSGLFANPRKSSIFIAGVNNENASRLAACMGFVRGNL 578

BLAST of PI0007006 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 74.3 bits (181), Expect = 1.0e-13
Identity = 40/80 (50.00%), Postives = 53/80 (66.25%), Query Frame = 0

Query: 87  MAGVDIEVATVLADSMGFALGTLPVRYLGVPLLSGRLRSSNCAPLIQRITSRIRSWSARV 146
           MAGV       +  S  FA G LPVRYLG+PLL+ ++ +S+  PL+++I  RI  W+AR 
Sbjct: 1   MAGVKDNDKADILHSFPFASGALPVRYLGLPLLTKKMTTSDYGPLVEKIRVRIGKWTARH 60

Query: 147 LSFAGRLQLVRSVLRSFQVF 167
           LSFAGRLQL+ SV+ S   F
Sbjct: 61  LSFAGRLQLISSVIHSLTNF 80

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7V4C13.4e-5763.08Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S4E2581.6e-5462.83uncharacterized protein LOC103496880 OS=Cucumis melo OX=3656 GN=LOC103496880 PE=... [more]
A0A1S3BSI83.3e-5252.32uncharacterized protein LOC103493225 OS=Cucumis melo OX=3656 GN=LOC103493225 PE=... [more]
A0A5A7V5J21.0e-5052.65Non-LTR retroelement reverse transcriptase-like protein OS=Cucumis melo var. mak... [more]
A0A5A7UP657.5e-4946.62Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43... [more]
Match NameE-valueIdentityDescription
KAA0060725.17.0e-5763.08uncharacterized protein E6C27_scaffold72G00120 [Cucumis melo var. makuwa] >TYK01... [more]
XP_031740402.11.3e-5557.48uncharacterized protein LOC116403409 [Cucumis sativus] >XP_031740413.1 uncharact... [more]
XP_016902060.13.2e-5462.83PREDICTED: uncharacterized protein LOC103496880 [Cucumis melo][more]
XP_031745634.11.2e-5366.09uncharacterized protein LOC116406053 [Cucumis sativus][more]
XP_008452126.16.7e-5252.32PREDICTED: uncharacterized protein LOC103493225 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT3G24255.11.0e-1350.00RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 3..60
e-value: 1.9E-10
score: 40.7
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 2..66
coord: 71..164
NoneNo IPR availablePANTHERPTHR33116:SF33OS01G0885550 PROTEINcoord: 2..66
coord: 71..164

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0007006.1PI0007006.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane