PI0006864 (gene) Melon (PI 482460) v1

Overview
NamePI0006864
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionRHOMBOID-like protein
Locationchr09: 2279866 .. 2282772 (+)
RNA-Seq ExpressionPI0006864
SyntenyPI0006864
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTCTCCGACCCTTCGGTTTCAACTGAGGGGTATAATCGTCATTTCCTCTTCCGCCATTGTTTTCAATACAAGTAATTTTCAAAGCTTCCTCGCCTCCACCTGAACCACCGACTGCTACATATATAGAGAAACTGCGAACCAAAATCAAAATCCCCAATCAATTCATCAAAATACAAACACCACCATTAGAAATTTCTTCAAGAAAAACCCCCTTCAATTCCCTCTTCCATCATTGAGAAACCAAAGCTTGAAGCTTCTGTAGAGAAACAGAGTGGAAAGATGTCGAATCAAGATCTTGAGCGAGGAGCAGCTAAGAATCCACCCAATACTAACCCAAATGCTAACTATTCAACTTCTGCTTACTACATAGAGACTTCTGAGAAGCAATGGACGCCTTGGTTGGTTCCTATGTTTGTTGTGGCTAATATTGCTATGTTCATTGTGGTCATGTATGTCAATAATTGCCCCGACTCTAATCGGGGTGTTGGAGATAAGTGTGTGGCTGGTTTTCTTGGACGATTTTCGTTTCAGCCTATCAGGGAGAACCCCCTCTTGGGGCCTTCTTCCAATACGTATGTCTCTTCTTTTGCTCTGTTTTCCTCTTTATATCAATTATTGTTTTGTATTTTGTGTTTTGTGTTTTGCTTTGAGTATGCTTCTCTATGGGTTTTATTCTGTATGCTCCAGAATTTTTAATACATGAATTTTTTATGGAGAGTGTTTCTTCAATCTCCTTGGGTTTGATCCTGCTCTGTTTCTTCTTTTGTTTCATCTTAGTTTTGTGTTTGGATTTGAGAGTGATTATCTGTGGTTTGATTTTTTGTACTCCAAATTTAAGAGTTTTGTCTTGCCCTTTTAATAAGTGGTCTAGATTTAGTGATTATCTAGAACATAAGACATGAATTTCTGGATTGAGAGTCTTTTATCAATCTCCATGGGTTTGATTCTGCCTGTTTTTTTCTTTAGTCCATCTAAATTTTGCGTTTGGATTTTTAATTTAGTGTATTTCTACATGGGTTTGATTCTGTATGCTCCATGGTTTTCAAGTTTCGCTTTGCCCATGTGGTATAAAATTTAGTGATATCTCGAACATAATACATGAACTTAATTTCTTAATCTTTCTCCCCTTTGCATATTGTTTTAATTTGTAGATCCATGGGAATTGATGTTAATTGTTATATTACTGTTTCAAACAAATATTCAAAACGTCTTTCGATTTGTATATGAAAAATTCTATGGATTGGATCCTATTCCCCTTAATTGAATTCATTTGCCTAAAAAAAACATTTTTATTCTGCAGATTGGTGAAATTGGGAGCTCTGAAGTGGAATAAGGTTGTTCATGAGCATCAAGGATGGAGACTTTTCTCCTGTATCTGGTTGCACGCTGGAATCATTCATCTTCTTGCTAATATGTTGAGTTTGGTTCTGATTGGAATCCGCCTCGAGCAACAATTTGGATTTGGTATGTCACTTGTAAATTTTCTAAATATTGTTGGACTTTGTATCATGTTCTGTATTGTCTCAGCAAATTGTCGTAGAACTAGAATAAAATTCAAAGAGATGTAGCTTTTCAGTTACCTCCAAAAGAGAACTGCTTTTTTAAGGGAGTTTCATAAATCTAAACTTGAATACATGGCGAAATTAATACCCACAAAGTCGTGACATGAGTTACAATTTCTTTGTTATAAACATTTATATGGTCGAATAAGGTGGACTTTGTAACCATCATGAGTTGACCTAATAAAGAGAAATGACGGTCACCTACCTAAGAATTAATTTCCCACAACTTTCTTAGACACCGAAATATAGTAGGATCTGTCGAGGTGGACATAAGCTGATTCGGTATCTCACAGGTATAAAAAAGAAATAAATAAGGTGGACTTTGTGGGTGCAGCTTTTCTGATTATATAGGAATCTAGAATATTTTATGGCTGAGTTTGGATTTTCCCATCTTTGTGTGAACGTTTATATTCATTTTTGCAGTGCGTATCGGGCTAATATACCTTGTGGCTGGAGTTGGTGGGAGTGTGATGTCTTCTCTTTTCATCCAAAACAATATTTCTGTTGGTGCTTCTGGTGCCCTCTTTGGACTTCTTGGAGCTATGCTATCCGAGCTTTTGACAAACTGGACAATTTACACCAACAAGGTTAGCTGCCAGGCATCAACTTCGACATTGATTTTCTCCGTTTGTAATGGTTTTATCTGTGTATTTATGTTGTGACATGTTCAATAGCTAAAAGACTAATTGTGTTTTTGTAGGCTGCTGCACTCTTCACCCTCATAGTCATCGTTGTTATTAACTTAGCAGTTGGAATTCTACCTCACGTCGACAATTTTGCCCACATTGGAGGATTCTTAACAGGGTTCCTTCTCGGGTTTGTATTGCTTGTCCGTCCACAGTTCAAGTGGACAGAGCGCCACCATCTCCCACCTAGTGCTCAACGTGTACCCAAGTATAAAACTTATCAGTATATTCTCTGGCTGGCAGCTGCAATTCTACTAGTTGCTGGGTAAAAGCTCTTAACCCTTGCCTTAACTTGGATTCTTCGACTGCTTGTCAACATGTTTAAATCAGAGTAAGCTAATTCAGTTTGATATTTGTTGCAGCTTTACCCTGGGCCTGGTGATGCTATTCAGAGGTGAGAATGGAAACAAGCACTGCAGCTGGTGCCATTACCTGAGTTGTGTGCCAACCTCGAGATGGGATTGCGCAAATTAAGTTTCTTTTGATTTGTGCGTACATAGTTACACTTTTTGGGCTCTCGGGTTTTAGTTTGCTTTTTACTTCATGTATATGAATAGTTTGAGGCTTTTTTTTTTCTTTTCCCGCCTTGTCTTTACAATTGTTTGCAGTAGAGATCTGTGATCTATTTCAGATTAATAATATTGATTATTGCCTTTC

mRNA sequence

CTTTCTCCGACCCTTCGGTTTCAACTGAGGGGTATAATCGTCATTTCCTCTTCCGCCATTGTTTTCAATACAAGTAATTTTCAAAGCTTCCTCGCCTCCACCTGAACCACCGACTGCTACATATATAGAGAAACTGCGAACCAAAATCAAAATCCCCAATCAATTCATCAAAATACAAACACCACCATTAGAAATTTCTTCAAGAAAAACCCCCTTCAATTCCCTCTTCCATCATTGAGAAACCAAAGCTTGAAGCTTCTGTAGAGAAACAGAGTGGAAAGATGTCGAATCAAGATCTTGAGCGAGGAGCAGCTAAGAATCCACCCAATACTAACCCAAATGCTAACTATTCAACTTCTGCTTACTACATAGAGACTTCTGAGAAGCAATGGACGCCTTGGTTGGTTCCTATGTTTGTTGTGGCTAATATTGCTATGTTCATTGTGGTCATGTATGTCAATAATTGCCCCGACTCTAATCGGGGTGTTGGAGATAAGTGTGTGGCTGGTTTTCTTGGACGATTTTCGTTTCAGCCTATCAGGGAGAACCCCCTCTTGGGGCCTTCTTCCAATACATTGGTGAAATTGGGAGCTCTGAAGTGGAATAAGGTTGTTCATGAGCATCAAGGATGGAGACTTTTCTCCTGTATCTGGTTGCACGCTGGAATCATTCATCTTCTTGCTAATATGTTGAGTTTGGTTCTGATTGGAATCCGCCTCGAGCAACAATTTGGATTTGTGCGTATCGGGCTAATATACCTTGTGGCTGGAGTTGGTGGGAGTGTGATGTCTTCTCTTTTCATCCAAAACAATATTTCTGTTGGTGCTTCTGGTGCCCTCTTTGGACTTCTTGGAGCTATGCTATCCGAGCTTTTGACAAACTGGACAATTTACACCAACAAGGCTGCTGCACTCTTCACCCTCATAGTCATCGTTGTTATTAACTTAGCAGTTGGAATTCTACCTCACGTCGACAATTTTGCCCACATTGGAGGATTCTTAACAGGGTTCCTTCTCGGGTTTGTATTGCTTGTCCGTCCACAGTTCAAGTGGACAGAGCGCCACCATCTCCCACCTAGTGCTCAACGTGTACCCAAGTATAAAACTTATCAGTATATTCTCTGGCTGGCAGCTGCAATTCTACTAGTTGCTGGCTTTACCCTGGGCCTGGTGATGCTATTCAGAGGTGAGAATGGAAACAAGCACTGCAGCTGGTGCCATTACCTGAGTTGTGTGCCAACCTCGAGATGGGATTGCGCAAATTAAGTTTCTTTTGATTTGTGCGTACATAGTTACACTTTTTGGGCTCTCGGGTTTTAGTTTGCTTTTTACTTCATGTATATGAATAGTTTGAGGCTTTTTTTTTTCTTTTCCCGCCTTGTCTTTACAATTGTTTGCAGTAGAGATCTGTGATCTATTTCAGATTAATAATATTGATTATTGCCTTTC

Coding sequence (CDS)

ATGTCGAATCAAGATCTTGAGCGAGGAGCAGCTAAGAATCCACCCAATACTAACCCAAATGCTAACTATTCAACTTCTGCTTACTACATAGAGACTTCTGAGAAGCAATGGACGCCTTGGTTGGTTCCTATGTTTGTTGTGGCTAATATTGCTATGTTCATTGTGGTCATGTATGTCAATAATTGCCCCGACTCTAATCGGGGTGTTGGAGATAAGTGTGTGGCTGGTTTTCTTGGACGATTTTCGTTTCAGCCTATCAGGGAGAACCCCCTCTTGGGGCCTTCTTCCAATACATTGGTGAAATTGGGAGCTCTGAAGTGGAATAAGGTTGTTCATGAGCATCAAGGATGGAGACTTTTCTCCTGTATCTGGTTGCACGCTGGAATCATTCATCTTCTTGCTAATATGTTGAGTTTGGTTCTGATTGGAATCCGCCTCGAGCAACAATTTGGATTTGTGCGTATCGGGCTAATATACCTTGTGGCTGGAGTTGGTGGGAGTGTGATGTCTTCTCTTTTCATCCAAAACAATATTTCTGTTGGTGCTTCTGGTGCCCTCTTTGGACTTCTTGGAGCTATGCTATCCGAGCTTTTGACAAACTGGACAATTTACACCAACAAGGCTGCTGCACTCTTCACCCTCATAGTCATCGTTGTTATTAACTTAGCAGTTGGAATTCTACCTCACGTCGACAATTTTGCCCACATTGGAGGATTCTTAACAGGGTTCCTTCTCGGGTTTGTATTGCTTGTCCGTCCACAGTTCAAGTGGACAGAGCGCCACCATCTCCCACCTAGTGCTCAACGTGTACCCAAGTATAAAACTTATCAGTATATTCTCTGGCTGGCAGCTGCAATTCTACTAGTTGCTGGCTTTACCCTGGGCCTGGTGATGCTATTCAGAGGTGAGAATGGAAACAAGCACTGCAGCTGGTGCCATTACCTGAGTTGTGTGCCAACCTCGAGATGGGATTGCGCAAATTAA

Protein sequence

MSNQDLERGAAKNPPNTNPNANYSTSAYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVNNCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLFSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGLIYLVAGVGGSVMSSLFIQNNISVGASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGFLTGFLLGFVLLVRPQFKWTERHHLPPSAQRVPKYKTYQYILWLAAAILLVAGFTLGLVMLFRGENGNKHCSWCHYLSCVPTSRWDCAN
Homology
BLAST of PI0006864 vs. ExPASy Swiss-Prot
Match: Q9CAN1 (RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 7.2e-125
Identity = 219/326 (67.18%), Postives = 260/326 (79.75%), Query Frame = 0

Query: 1   MSNQDLERGAAKNPPNTNPNANYSTSAYYIETS-EKQWTPWLVPMFVVANIAMFIVVMYV 60
           M+N+D+ER   K   N   N NY    +Y E+S E  WT WL+P  VVAN+A+FI VM+V
Sbjct: 1   MANRDVERVGKK---NRGANNNY----FYEESSGETHWTSWLIPAIVVANLAVFIAVMFV 60

Query: 61  NNCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRL 120
           N+CP    G   +CVA FLGRFSFQP++ENPL GPSS+TL K+GAL+W KVVHEHQGWRL
Sbjct: 61  NDCPKKITGPNKECVARFLGRFSFQPLKENPLFGPSSSTLEKMGALEWRKVVHEHQGWRL 120

Query: 121 FSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGLIYLVAGVGGSVMSSLFIQNNIS 180
            SC+WLHAGIIHLL NMLSL+ IGIRLEQQFGF+R+GLIYL++G+GGS++SSLF+Q +IS
Sbjct: 121 LSCMWLHAGIIHLLTNMLSLIFIGIRLEQQFGFIRVGLIYLISGLGGSILSSLFLQESIS 180

Query: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGF 240
           VGASGALFGLLGAMLSELLTNWTIY NKAAAL TL+ I+ INLA+G+LP VDNFAHIGGF
Sbjct: 181 VGASGALFGLLGAMLSELLTNWTIYANKAAALITLLFIIAINLALGMLPRVDNFAHIGGF 240

Query: 241 LTGFLLGFVLLVRPQFKWTERHHLPPSAQRVPKYKTYQYILWLAAAILLVAGFTLGLVML 300
           LTGF LGFVLLVRPQ+ W          +R  KY  YQY+L++ + +LLV G T+ LVML
Sbjct: 241 LTGFCLGFVLLVRPQYGWEASRTNTSRTKR--KYSMYQYVLFVVSVVLLVVGLTVALVML 300

Query: 301 FRGENGNKHCSWCHYLSCVPTSRWDC 326
           F+GENGNKHC WCHYLSC PTS+W C
Sbjct: 301 FKGENGNKHCKWCHYLSCFPTSKWTC 317

BLAST of PI0006864 vs. ExPASy Swiss-Prot
Match: Q9LYP1 (RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 3.6e-124
Identity = 214/322 (66.46%), Postives = 259/322 (80.43%), Query Frame = 0

Query: 8   RGAAKN--PPNTNPNANYSTSAYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVNNCPD- 67
           RG  +N   P   P A  S++ +       +WT WLVPMFVVAN+A+F+V M+VNNCP+ 
Sbjct: 22  RGGDRNRIGPPPLPVALSSSTEFGDNALSSRWTSWLVPMFVVANVAVFVVAMFVNNCPNH 81

Query: 68  -SNRGVGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLFSCI 127
             +  +   CVA FLGR SF+P+R NPL GPSS+TL KLGAL+W+KVV + +GWRL +CI
Sbjct: 82  FESHRLRGHCVAKFLGRLSFEPLRTNPLFGPSSHTLEKLGALEWSKVVEKKEGWRLLTCI 141

Query: 128 WLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGLIYLVAGVGGSVMSSLFIQNNISVGAS 187
           WLHAG+IHL ANMLSLV IGIRLEQQFGFVRIG+IYL++G+GGSV+SSLFI+N+ISVGAS
Sbjct: 142 WLHAGVIHLGANMLSLVFIGIRLEQQFGFVRIGVIYLLSGIGGSVLSSLFIRNSISVGAS 201

Query: 188 GALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGFLTGF 247
           GALFGLLG+MLSEL TNWTIY+NK AAL TL+ +++INLA+GILPHVDNFAH+GGF+TGF
Sbjct: 202 GALFGLLGSMLSELFTNWTIYSNKIAALLTLLFVILINLAIGILPHVDNFAHVGGFVTGF 261

Query: 248 LLGFVLLVRPQFKWTERHHLPPSAQRVPKYKTYQYILWLAAAILLVAGFTLGLVMLFRGE 307
           LLGF+LL RPQFKW  R H+P       KYKTYQY+LWL + +LL+AGF + L+MLFRGE
Sbjct: 262 LLGFILLARPQFKWLAREHMPQGTPLRYKYKTYQYLLWLLSLVLLIAGFVVALLMLFRGE 321

Query: 308 NGNKHCSWCHYLSCVPTSRWDC 326
           NGN HC WCHYL CVPTS W C
Sbjct: 322 NGNDHCRWCHYLRCVPTSSWRC 343

BLAST of PI0006864 vs. ExPASy Swiss-Prot
Match: Q0WQX7 (RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 2.4e-112
Identity = 196/296 (66.22%), Postives = 241/296 (81.42%), Query Frame = 0

Query: 35  KQWTPWLVPMFVVANIAMFIVVMYVNNCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLGP 94
           K W PWLVP  VVANIA+F + M++NNCP ++      C+A FLGRF+FQP++ENPLLGP
Sbjct: 52  KLWFPWLVPAIVVANIALFAISMFINNCPKNSA----YCLARFLGRFAFQPMKENPLLGP 111

Query: 95  SSNTLVKLGALKWNKVVHEHQGWRLFSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVR 154
           SS TL K+GAL  + VVH+H+ WRLF+CIWLHAG+ H+LANMLSL+ IGIRLEQ+FGFVR
Sbjct: 112 SSLTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFVR 171

Query: 155 IGLIYLVAGVGGSVMSSLFIQNNISVGASGALFGLLGAMLSELLTNWTIYTNKAAALFTL 214
           IGL+Y+++G GGS++SSLF +  ISVGASGALFGLLGAMLSELLTNWTIY NK AAL TL
Sbjct: 172 IGLLYMISGFGGSLLSSLFNRAGISVGASGALFGLLGAMLSELLTNWTIYANKFAALLTL 231

Query: 215 IVIVVINLAVGILPHVDNFAHIGGFLTGFLLGFVLLVRPQFKWTERHHLP-----PSAQR 274
           I I+ INLAVGILPHVDNFAH+GGF +GFLLGFV L+RPQ+ +  + + P     PSA+ 
Sbjct: 232 IFIIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIRPQYGYFNQRNNPRGYAAPSAK- 291

Query: 275 VPKYKTYQYILWLAAAILLVAGFTLGLVMLFRGENGNKHCSWCHYLSCVPTSRWDC 326
             K+K YQY+LW+ + +LL+AG+T GLV+L RG + NKHCSWCHYLSC+PTS W C
Sbjct: 292 -SKHKPYQYVLWITSLVLLIAGYTAGLVVLLRGTDLNKHCSWCHYLSCIPTSLWSC 341

BLAST of PI0006864 vs. ExPASy Swiss-Prot
Match: Q8VZ48 (RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL6 PE=2 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 1.0e-107
Identity = 185/293 (63.14%), Postives = 227/293 (77.47%), Query Frame = 0

Query: 34  EKQWTPWLVPMFVVANIAMFIVVMYVNNCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLG 93
           + QWT WL P  VVAN+++FIVVMY N+CP +  G    CVA  L RFSFQP+RENP LG
Sbjct: 15  DTQWTAWLTPTIVVANVSIFIVVMYTNDCPKTTTGANGDCVAKLLRRFSFQPLRENPFLG 74

Query: 94  PSSNTLVKLGALKWNKVVHEHQGWRLFSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFV 153
           PSS+TL KLGAL W KVV  ++ WRL + +WLHAGIIHL+ NM  +++ GIRLEQQFGF+
Sbjct: 75  PSSSTLEKLGALDWKKVVQGNEKWRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGFI 134

Query: 154 RIGLIYLVAGVGGSVMSSLFIQNNISVGASGALFGLLGAMLSELLTNWTIYTNKAAALFT 213
           RIGLIYL++G GGS++S+LF+Q +ISVGASGAL GL+GAMLSELLTNWTIY +K  AL +
Sbjct: 135 RIGLIYLISGFGGSILSALFLQKSISVGASGALLGLMGAMLSELLTNWTIYKSKLCALLS 194

Query: 214 LIVIVVINLAVGILPHVDNFAHIGGFLTGFLLGFVLLVRPQFKWTE-RHHLPPSAQRVPK 273
            + I+ INLA+G+LP VDNFAHIGG LTGF LGF+LL++PQ  W E R+     A+   K
Sbjct: 195 FLFIIAINLAIGLLPWVDNFAHIGGLLTGFCLGFILLMQPQSGWEEFRNSSQYGARARSK 254

Query: 274 YKTYQYILWLAAAILLVAGFTLGLVMLFRGENGNKHCSWCHYLSCVPTSRWDC 326
           Y   QY+L+  AA+L+VAG T+GLVMLF GENGNKHC WCH L C PTS+W C
Sbjct: 255 YNPCQYVLFFVAAVLVVAGLTVGLVMLFDGENGNKHCKWCHRLDCYPTSKWSC 307

BLAST of PI0006864 vs. ExPASy Swiss-Prot
Match: F4JBM4 (RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 1.4e-104
Identity = 177/291 (60.82%), Postives = 227/291 (78.01%), Query Frame = 0

Query: 35  KQWTPWLVPMFVVANIAMFIVVMYVNNCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLGP 94
           + W PWL+P FVVAN+A+F++ MYVNNCP  +   GD C A FLGRFSFQ  RENPLLGP
Sbjct: 62  RSWFPWLIPCFVVANVAVFVITMYVNNCPKKS---GD-CFADFLGRFSFQNTRENPLLGP 121

Query: 95  SSNTLVKLGALKWNKVVHEHQGWRLFSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVR 154
           SS TL  +G L   KVV   +GWRL SC WLH G++HLL NML+L+ IGIR+E++FGF+R
Sbjct: 122 SSLTLQTMGGLDVKKVVKGDEGWRLLSCNWLHGGVVHLLMNMLTLLFIGIRMEREFGFIR 181

Query: 155 IGLIYLVAGVGGSVMSSLFIQNNISVGASGALFGLLGAMLSELLTNWTIYTNKAAALFTL 214
           IGL+YL++G GGS++S+LF+++NISVGASGA+FGLLG MLSE+  NWTIY+NK   + TL
Sbjct: 182 IGLLYLISGFGGSILSALFLRSNISVGASGAVFGLLGGMLSEIFINWTIYSNKVVTIVTL 241

Query: 215 IVIVVINLAVGILPHVDNFAHIGGFLTGFLLGFVLLVRPQFKWTERHHLPPSAQRVPKYK 274
           ++IV +NL +G+LP VDNFAHIGGF TGFLLGFVLL+RP + W  + + P    +  ++K
Sbjct: 242 VLIVAVNLGLGVLPGVDNFAHIGGFATGFLLGFVLLIRPHYGWINQRNGP--GAKPHRFK 301

Query: 275 TYQYILWLAAAILLVAGFTLGLVMLFRGENGNKHCSWCHYLSCVPTSRWDC 326
            YQ ILW  + ++LVAGF +GL+ LF   +GN+HCSWCHYLSCVPTS+W C
Sbjct: 302 IYQGILWTISLLILVAGFIVGLISLFNNVDGNEHCSWCHYLSCVPTSKWSC 346

BLAST of PI0006864 vs. ExPASy TrEMBL
Match: A0A5A7TMJ5 (RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002940 PE=3 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.2e-183
Identity = 317/327 (96.94%), Postives = 324/327 (99.08%), Query Frame = 0

Query: 1   MSNQDLERGAAKNPPNTNPNANYSTSAYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60
           MSNQDLERGAAKNPPNTNPNANYS+S+YYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPPNTNPNANYSSSSYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLF 120
           NCP+SN G GDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLF
Sbjct: 61  NCPNSNLGFGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLF 120

Query: 121 SCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGLIYLVAGVGGSVMSSLFIQNNISV 180
           SCIWLHAGIIHLLANMLSLV+IGIRLEQQFGFVRIG+IYLVAGVGGSVMSSLFIQNNISV
Sbjct: 121 SCIWLHAGIIHLLANMLSLVVIGIRLEQQFGFVRIGIIYLVAGVGGSVMSSLFIQNNISV 180

Query: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGFL 240
           GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVI+VINLAVGILPHVDNFAHIGGFL
Sbjct: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIIVINLAVGILPHVDNFAHIGGFL 240

Query: 241 TGFLLGFVLLVRPQFKWTERHHLPPSAQRVPKYKTYQYILWLAAAILLVAGFTLGLVMLF 300
           TGFLLGFVLLVRPQFKWTERHHLPP AQRVPKYKTYQYILWLAAAI+LVAGFTLGLVMLF
Sbjct: 241 TGFLLGFVLLVRPQFKWTERHHLPPGAQRVPKYKTYQYILWLAAAIVLVAGFTLGLVMLF 300

Query: 301 RGENGNKHCSWCHYLSCVPTSRWDCAN 328
           RGENGNKHCSWCHYLSCVPTSRWDCAN
Sbjct: 301 RGENGNKHCSWCHYLSCVPTSRWDCAN 327

BLAST of PI0006864 vs. ExPASy TrEMBL
Match: A0A1S3AU27 (RHOMBOID-like protein OS=Cucumis melo OX=3656 GN=LOC103482712 PE=3 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.2e-183
Identity = 317/327 (96.94%), Postives = 324/327 (99.08%), Query Frame = 0

Query: 1   MSNQDLERGAAKNPPNTNPNANYSTSAYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60
           MSNQDLERGAAKNPPNTNPNANYS+S+YYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPPNTNPNANYSSSSYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLF 120
           NCP+SN G GDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLF
Sbjct: 61  NCPNSNLGFGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLF 120

Query: 121 SCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGLIYLVAGVGGSVMSSLFIQNNISV 180
           SCIWLHAGIIHLLANMLSLV+IGIRLEQQFGFVRIG+IYLVAGVGGSVMSSLFIQNNISV
Sbjct: 121 SCIWLHAGIIHLLANMLSLVVIGIRLEQQFGFVRIGIIYLVAGVGGSVMSSLFIQNNISV 180

Query: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGFL 240
           GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVI+VINLAVGILPHVDNFAHIGGFL
Sbjct: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIIVINLAVGILPHVDNFAHIGGFL 240

Query: 241 TGFLLGFVLLVRPQFKWTERHHLPPSAQRVPKYKTYQYILWLAAAILLVAGFTLGLVMLF 300
           TGFLLGFVLLVRPQFKWTERHHLPP AQRVPKYKTYQYILWLAAAI+LVAGFTLGLVMLF
Sbjct: 241 TGFLLGFVLLVRPQFKWTERHHLPPGAQRVPKYKTYQYILWLAAAIVLVAGFTLGLVMLF 300

Query: 301 RGENGNKHCSWCHYLSCVPTSRWDCAN 328
           RGENGNKHCSWCHYLSCVPTSRWDCAN
Sbjct: 301 RGENGNKHCSWCHYLSCVPTSRWDCAN 327

BLAST of PI0006864 vs. ExPASy TrEMBL
Match: A0A0A0KMN4 (RHOMBOID-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G155500 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 1.0e-182
Identity = 317/327 (96.94%), Postives = 320/327 (97.86%), Query Frame = 0

Query: 1   MSNQDLERGAAKNPPNTNPNANYSTSAYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60
           MSNQDLERGAAKNPPNTNPNANYSTS YY+ETSEKQWTPWLVPMFVVANIAMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPPNTNPNANYSTSPYYLETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLF 120
           NCPDSN G GDKCVA FLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRL 
Sbjct: 61  NCPDSNLGFGDKCVASFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLL 120

Query: 121 SCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGLIYLVAGVGGSVMSSLFIQNNISV 180
           SCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIG+IYLVAGVGGSVMSSLFIQNNISV
Sbjct: 121 SCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGMIYLVAGVGGSVMSSLFIQNNISV 180

Query: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGFL 240
           GASGALFGLLGAMLSELLTNWTIYTNK AALFTLIVIVVINLAVGILPHVDNFAHIGGFL
Sbjct: 181 GASGALFGLLGAMLSELLTNWTIYTNKVAALFTLIVIVVINLAVGILPHVDNFAHIGGFL 240

Query: 241 TGFLLGFVLLVRPQFKWTERHHLPPSAQRVPKYKTYQYILWLAAAILLVAGFTLGLVMLF 300
           TGFLLGFVLLVRPQFKWTERHHLPP A+RVPKYKTYQYILWLAAAILLVAGFTLGLVMLF
Sbjct: 241 TGFLLGFVLLVRPQFKWTERHHLPPGARRVPKYKTYQYILWLAAAILLVAGFTLGLVMLF 300

Query: 301 RGENGNKHCSWCHYLSCVPTSRWDCAN 328
           RGENGNKHCSWCHYLSCVPTSRWDCAN
Sbjct: 301 RGENGNKHCSWCHYLSCVPTSRWDCAN 327

BLAST of PI0006864 vs. ExPASy TrEMBL
Match: A0A6J1H457 (RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111459437 PE=3 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 1.7e-174
Identity = 300/327 (91.74%), Postives = 312/327 (95.41%), Query Frame = 0

Query: 1   MSNQDLERGAAKNPPNTNPNANYSTSAYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60
           MSNQDLERGAAKNPPN+NP  NYS   Y++ETSEKQWT WLVPMFVVANIAMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPPNSNPTGNYSAPPYHVETSEKQWTAWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLF 120
           NCP +N G GDKCVAGFLGRFSFQP++ENPLLGPSSNTLVKLGALKW+KVVHEHQGWRL 
Sbjct: 61  NCPKTNLGFGDKCVAGFLGRFSFQPLKENPLLGPSSNTLVKLGALKWDKVVHEHQGWRLL 120

Query: 121 SCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGLIYLVAGVGGSVMSSLFIQNNISV 180
           SCIWLHAGIIHLLANMLSLV+IGIRLEQQFGFVRIG+IYLVAGVGGSVMSSLFIQNNISV
Sbjct: 121 SCIWLHAGIIHLLANMLSLVVIGIRLEQQFGFVRIGIIYLVAGVGGSVMSSLFIQNNISV 180

Query: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGFL 240
           GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIV INLAVGILPHVDNFAHIGGFL
Sbjct: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVAINLAVGILPHVDNFAHIGGFL 240

Query: 241 TGFLLGFVLLVRPQFKWTERHHLPPSAQRVPKYKTYQYILWLAAAILLVAGFTLGLVMLF 300
           TGFLLGFVLL+RPQFKW ERHHLPPSAQRVPKYK YQYILW+ AAILLVAGFT+GLVMLF
Sbjct: 241 TGFLLGFVLLIRPQFKWIERHHLPPSAQRVPKYKIYQYILWIVAAILLVAGFTVGLVMLF 300

Query: 301 RGENGNKHCSWCHYLSCVPTSRWDCAN 328
           RGENGNKHCSWCHYLSCVPTSRWDC N
Sbjct: 301 RGENGNKHCSWCHYLSCVPTSRWDCGN 327

BLAST of PI0006864 vs. ExPASy TrEMBL
Match: A0A6J1JZC3 (RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111491076 PE=3 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 4.0e-171
Identity = 296/327 (90.52%), Postives = 310/327 (94.80%), Query Frame = 0

Query: 1   MSNQDLERGAAKNPPNTNPNANYSTSAYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60
           MSNQDLERGAAKNPPN+NP  NYS   Y++ETSEKQWTPWLVPMFVVANIAMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPPNSNPTGNYSAPPYHVETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLF 120
           NCP +N G  DKCVAGFLGRFSFQP++ENPLLGPSSNTLVKLGALKW+KVVHEHQGWRL 
Sbjct: 61  NCPKTNLGFEDKCVAGFLGRFSFQPLKENPLLGPSSNTLVKLGALKWDKVVHEHQGWRLL 120

Query: 121 SCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGLIYLVAGVGGSVMSSLFIQNNISV 180
           SCI LHAGIIHLL+NMLSLV+IGIRLEQQFGFV IG+IYLVAGVGGSVMSSLFIQN+ISV
Sbjct: 121 SCILLHAGIIHLLSNMLSLVVIGIRLEQQFGFVCIGIIYLVAGVGGSVMSSLFIQNSISV 180

Query: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGFL 240
           GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIV INLAVGILPHVDNFAHIGGFL
Sbjct: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVAINLAVGILPHVDNFAHIGGFL 240

Query: 241 TGFLLGFVLLVRPQFKWTERHHLPPSAQRVPKYKTYQYILWLAAAILLVAGFTLGLVMLF 300
           TGFLLGFVLL+RPQFKW ERHHLPPSAQRVPKYK YQYILW+ AAILLVAGFT+GLVMLF
Sbjct: 241 TGFLLGFVLLIRPQFKWIERHHLPPSAQRVPKYKIYQYILWIVAAILLVAGFTVGLVMLF 300

Query: 301 RGENGNKHCSWCHYLSCVPTSRWDCAN 328
           RGENGNKHCSWCHYLSCVPTSRWDC N
Sbjct: 301 RGENGNKHCSWCHYLSCVPTSRWDCGN 327

BLAST of PI0006864 vs. NCBI nr
Match: XP_008437215.1 (PREDICTED: RHOMBOID-like protein 2 [Cucumis melo] >KAA0042791.1 RHOMBOID-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 652.1 bits (1681), Expect = 2.5e-183
Identity = 317/327 (96.94%), Postives = 324/327 (99.08%), Query Frame = 0

Query: 1   MSNQDLERGAAKNPPNTNPNANYSTSAYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60
           MSNQDLERGAAKNPPNTNPNANYS+S+YYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPPNTNPNANYSSSSYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLF 120
           NCP+SN G GDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLF
Sbjct: 61  NCPNSNLGFGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLF 120

Query: 121 SCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGLIYLVAGVGGSVMSSLFIQNNISV 180
           SCIWLHAGIIHLLANMLSLV+IGIRLEQQFGFVRIG+IYLVAGVGGSVMSSLFIQNNISV
Sbjct: 121 SCIWLHAGIIHLLANMLSLVVIGIRLEQQFGFVRIGIIYLVAGVGGSVMSSLFIQNNISV 180

Query: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGFL 240
           GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVI+VINLAVGILPHVDNFAHIGGFL
Sbjct: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIIVINLAVGILPHVDNFAHIGGFL 240

Query: 241 TGFLLGFVLLVRPQFKWTERHHLPPSAQRVPKYKTYQYILWLAAAILLVAGFTLGLVMLF 300
           TGFLLGFVLLVRPQFKWTERHHLPP AQRVPKYKTYQYILWLAAAI+LVAGFTLGLVMLF
Sbjct: 241 TGFLLGFVLLVRPQFKWTERHHLPPGAQRVPKYKTYQYILWLAAAIVLVAGFTLGLVMLF 300

Query: 301 RGENGNKHCSWCHYLSCVPTSRWDCAN 328
           RGENGNKHCSWCHYLSCVPTSRWDCAN
Sbjct: 301 RGENGNKHCSWCHYLSCVPTSRWDCAN 327

BLAST of PI0006864 vs. NCBI nr
Match: XP_004143929.1 (RHOMBOID-like protein 2 [Cucumis sativus] >KGN50139.1 hypothetical protein Csa_000665 [Cucumis sativus])

HSP 1 Score: 649.0 bits (1673), Expect = 2.1e-182
Identity = 317/327 (96.94%), Postives = 320/327 (97.86%), Query Frame = 0

Query: 1   MSNQDLERGAAKNPPNTNPNANYSTSAYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60
           MSNQDLERGAAKNPPNTNPNANYSTS YY+ETSEKQWTPWLVPMFVVANIAMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPPNTNPNANYSTSPYYLETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLF 120
           NCPDSN G GDKCVA FLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRL 
Sbjct: 61  NCPDSNLGFGDKCVASFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLL 120

Query: 121 SCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGLIYLVAGVGGSVMSSLFIQNNISV 180
           SCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIG+IYLVAGVGGSVMSSLFIQNNISV
Sbjct: 121 SCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGMIYLVAGVGGSVMSSLFIQNNISV 180

Query: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGFL 240
           GASGALFGLLGAMLSELLTNWTIYTNK AALFTLIVIVVINLAVGILPHVDNFAHIGGFL
Sbjct: 181 GASGALFGLLGAMLSELLTNWTIYTNKVAALFTLIVIVVINLAVGILPHVDNFAHIGGFL 240

Query: 241 TGFLLGFVLLVRPQFKWTERHHLPPSAQRVPKYKTYQYILWLAAAILLVAGFTLGLVMLF 300
           TGFLLGFVLLVRPQFKWTERHHLPP A+RVPKYKTYQYILWLAAAILLVAGFTLGLVMLF
Sbjct: 241 TGFLLGFVLLVRPQFKWTERHHLPPGARRVPKYKTYQYILWLAAAILLVAGFTLGLVMLF 300

Query: 301 RGENGNKHCSWCHYLSCVPTSRWDCAN 328
           RGENGNKHCSWCHYLSCVPTSRWDCAN
Sbjct: 301 RGENGNKHCSWCHYLSCVPTSRWDCAN 327

BLAST of PI0006864 vs. NCBI nr
Match: XP_038874691.1 (RHOMBOID-like protein 2 [Benincasa hispida])

HSP 1 Score: 637.1 bits (1642), Expect = 8.3e-179
Identity = 310/327 (94.80%), Postives = 319/327 (97.55%), Query Frame = 0

Query: 1   MSNQDLERGAAKNPPNTNPNANYSTSAYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60
           MSNQDLERGAAKNPPNTNPN NYSTS YYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPPNTNPNGNYSTSPYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLF 120
           +CP+SN G  DKCVAGFLGRFSFQP++ENPLLGPSSNTLVKLGALKW+KVVHEHQGWRLF
Sbjct: 61  DCPNSNLGFRDKCVAGFLGRFSFQPLKENPLLGPSSNTLVKLGALKWDKVVHEHQGWRLF 120

Query: 121 SCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGLIYLVAGVGGSVMSSLFIQNNISV 180
           SCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIG+IYLVAGVGGSVMSSLFIQNNISV
Sbjct: 121 SCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGIIYLVAGVGGSVMSSLFIQNNISV 180

Query: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGFL 240
           GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGFL
Sbjct: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGFL 240

Query: 241 TGFLLGFVLLVRPQFKWTERHHLPPSAQRVPKYKTYQYILWLAAAILLVAGFTLGLVMLF 300
           TGFLLGFVLLVRPQFKWTERH+LPP AQRVPKYKTYQYILWLAAAILLVAG T+G+VMLF
Sbjct: 241 TGFLLGFVLLVRPQFKWTERHYLPPDAQRVPKYKTYQYILWLAAAILLVAGLTVGMVMLF 300

Query: 301 RGENGNKHCSWCHYLSCVPTSRWDCAN 328
           RGENGNKHCSWCHYLSCVPTSRWDC N
Sbjct: 301 RGENGNKHCSWCHYLSCVPTSRWDCGN 327

BLAST of PI0006864 vs. NCBI nr
Match: XP_023533312.1 (RHOMBOID-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 624.8 bits (1610), Expect = 4.3e-175
Identity = 301/327 (92.05%), Postives = 313/327 (95.72%), Query Frame = 0

Query: 1   MSNQDLERGAAKNPPNTNPNANYSTSAYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60
           MSNQDLERGAAKNPPN+NP  NYS   Y++ETSEKQWTPWLVPMFVVANIAMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPPNSNPTGNYSAPPYHVETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLF 120
           NCP +N G GDKCVAGFLGRFSFQP++ENPLLGPSSNTLVKLGALKW+KVVHEHQGWRL 
Sbjct: 61  NCPKTNLGFGDKCVAGFLGRFSFQPLKENPLLGPSSNTLVKLGALKWDKVVHEHQGWRLL 120

Query: 121 SCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGLIYLVAGVGGSVMSSLFIQNNISV 180
           SCIWLHAGIIHLLANMLSLV+IGIRLEQQFGFVRIG+IYLVAGVGGSVMSSLFIQNNISV
Sbjct: 121 SCIWLHAGIIHLLANMLSLVVIGIRLEQQFGFVRIGIIYLVAGVGGSVMSSLFIQNNISV 180

Query: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGFL 240
           GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIV INLAVGILPHVDNFAHIGGFL
Sbjct: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVAINLAVGILPHVDNFAHIGGFL 240

Query: 241 TGFLLGFVLLVRPQFKWTERHHLPPSAQRVPKYKTYQYILWLAAAILLVAGFTLGLVMLF 300
           TGFLLGFVLL+RPQFKW ERHHLPPSAQRVPKYK YQYILW+ AAILLVAGFT+GLVMLF
Sbjct: 241 TGFLLGFVLLIRPQFKWIERHHLPPSAQRVPKYKIYQYILWIVAAILLVAGFTVGLVMLF 300

Query: 301 RGENGNKHCSWCHYLSCVPTSRWDCAN 328
           RGENGNKHCSWCHYLSCVPTSRWDC N
Sbjct: 301 RGENGNKHCSWCHYLSCVPTSRWDCGN 327

BLAST of PI0006864 vs. NCBI nr
Match: KAG7036061.1 (RHOMBOID-like protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 623.6 bits (1607), Expect = 9.5e-175
Identity = 301/327 (92.05%), Postives = 313/327 (95.72%), Query Frame = 0

Query: 1   MSNQDLERGAAKNPPNTNPNANYSTSAYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60
           MSNQDLERGAAKNPPN+NP  NYS   Y++ETSEKQWTPWLVPMFVVANIAMFIVVMYVN
Sbjct: 1   MSNQDLERGAAKNPPNSNPTGNYSAPPYHVETSEKQWTPWLVPMFVVANIAMFIVVMYVN 60

Query: 61  NCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLF 120
           NCP +N GVGDKCVAGFLGRFSFQP++ENPLLGPSSNTLVKLGALKW+KVVHEHQGWRL 
Sbjct: 61  NCPKTNLGVGDKCVAGFLGRFSFQPLKENPLLGPSSNTLVKLGALKWDKVVHEHQGWRLL 120

Query: 121 SCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGLIYLVAGVGGSVMSSLFIQNNISV 180
           SCIWLHAGIIHLLANMLSLV+IGIRLEQQFGFVRIG+IYLVAGVGGSVMSSLFIQNNISV
Sbjct: 121 SCIWLHAGIIHLLANMLSLVVIGIRLEQQFGFVRIGIIYLVAGVGGSVMSSLFIQNNISV 180

Query: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGFL 240
           GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIV INLAVGILPHVDNFAHIGGFL
Sbjct: 181 GASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVAINLAVGILPHVDNFAHIGGFL 240

Query: 241 TGFLLGFVLLVRPQFKWTERHHLPPSAQRVPKYKTYQYILWLAAAILLVAGFTLGLVMLF 300
           TGFLLGFVLL+RPQFKW ERHHLPPSAQRV KYK YQYILW+ AAILLVAGFT+GLVMLF
Sbjct: 241 TGFLLGFVLLIRPQFKWIERHHLPPSAQRVSKYKIYQYILWIVAAILLVAGFTVGLVMLF 300

Query: 301 RGENGNKHCSWCHYLSCVPTSRWDCAN 328
           RGENGNKHCSWCHYLSCVPTSRWDC N
Sbjct: 301 RGENGNKHCSWCHYLSCVPTSRWDCGN 327

BLAST of PI0006864 vs. TAIR 10
Match: AT1G63120.1 (RHOMBOID-like 2 )

HSP 1 Score: 448.4 bits (1152), Expect = 5.1e-126
Identity = 219/326 (67.18%), Postives = 260/326 (79.75%), Query Frame = 0

Query: 1   MSNQDLERGAAKNPPNTNPNANYSTSAYYIETS-EKQWTPWLVPMFVVANIAMFIVVMYV 60
           M+N+D+ER   K   N   N NY    +Y E+S E  WT WL+P  VVAN+A+FI VM+V
Sbjct: 1   MANRDVERVGKK---NRGANNNY----FYEESSGETHWTSWLIPAIVVANLAVFIAVMFV 60

Query: 61  NNCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRL 120
           N+CP    G   +CVA FLGRFSFQP++ENPL GPSS+TL K+GAL+W KVVHEHQGWRL
Sbjct: 61  NDCPKKITGPNKECVARFLGRFSFQPLKENPLFGPSSSTLEKMGALEWRKVVHEHQGWRL 120

Query: 121 FSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGLIYLVAGVGGSVMSSLFIQNNIS 180
            SC+WLHAGIIHLL NMLSL+ IGIRLEQQFGF+R+GLIYL++G+GGS++SSLF+Q +IS
Sbjct: 121 LSCMWLHAGIIHLLTNMLSLIFIGIRLEQQFGFIRVGLIYLISGLGGSILSSLFLQESIS 180

Query: 181 VGASGALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGF 240
           VGASGALFGLLGAMLSELLTNWTIY NKAAAL TL+ I+ INLA+G+LP VDNFAHIGGF
Sbjct: 181 VGASGALFGLLGAMLSELLTNWTIYANKAAALITLLFIIAINLALGMLPRVDNFAHIGGF 240

Query: 241 LTGFLLGFVLLVRPQFKWTERHHLPPSAQRVPKYKTYQYILWLAAAILLVAGFTLGLVML 300
           LTGF LGFVLLVRPQ+ W          +R  KY  YQY+L++ + +LLV G T+ LVML
Sbjct: 241 LTGFCLGFVLLVRPQYGWEASRTNTSRTKR--KYSMYQYVLFVVSVVLLVVGLTVALVML 300

Query: 301 FRGENGNKHCSWCHYLSCVPTSRWDC 326
           F+GENGNKHC WCHYLSC PTS+W C
Sbjct: 301 FKGENGNKHCKWCHYLSCFPTSKWTC 317

BLAST of PI0006864 vs. TAIR 10
Match: AT5G07250.1 (RHOMBOID-like protein 3 )

HSP 1 Score: 446.0 bits (1146), Expect = 2.5e-125
Identity = 214/322 (66.46%), Postives = 259/322 (80.43%), Query Frame = 0

Query: 8   RGAAKN--PPNTNPNANYSTSAYYIETSEKQWTPWLVPMFVVANIAMFIVVMYVNNCPD- 67
           RG  +N   P   P A  S++ +       +WT WLVPMFVVAN+A+F+V M+VNNCP+ 
Sbjct: 22  RGGDRNRIGPPPLPVALSSSTEFGDNALSSRWTSWLVPMFVVANVAVFVVAMFVNNCPNH 81

Query: 68  -SNRGVGDKCVAGFLGRFSFQPIRENPLLGPSSNTLVKLGALKWNKVVHEHQGWRLFSCI 127
             +  +   CVA FLGR SF+P+R NPL GPSS+TL KLGAL+W+KVV + +GWRL +CI
Sbjct: 82  FESHRLRGHCVAKFLGRLSFEPLRTNPLFGPSSHTLEKLGALEWSKVVEKKEGWRLLTCI 141

Query: 128 WLHAGIIHLLANMLSLVLIGIRLEQQFGFVRIGLIYLVAGVGGSVMSSLFIQNNISVGAS 187
           WLHAG+IHL ANMLSLV IGIRLEQQFGFVRIG+IYL++G+GGSV+SSLFI+N+ISVGAS
Sbjct: 142 WLHAGVIHLGANMLSLVFIGIRLEQQFGFVRIGVIYLLSGIGGSVLSSLFIRNSISVGAS 201

Query: 188 GALFGLLGAMLSELLTNWTIYTNKAAALFTLIVIVVINLAVGILPHVDNFAHIGGFLTGF 247
           GALFGLLG+MLSEL TNWTIY+NK AAL TL+ +++INLA+GILPHVDNFAH+GGF+TGF
Sbjct: 202 GALFGLLGSMLSELFTNWTIYSNKIAALLTLLFVILINLAIGILPHVDNFAHVGGFVTGF 261

Query: 248 LLGFVLLVRPQFKWTERHHLPPSAQRVPKYKTYQYILWLAAAILLVAGFTLGLVMLFRGE 307
           LLGF+LL RPQFKW  R H+P       KYKTYQY+LWL + +LL+AGF + L+MLFRGE
Sbjct: 262 LLGFILLARPQFKWLAREHMPQGTPLRYKYKTYQYLLWLLSLVLLIAGFVVALLMLFRGE 321

Query: 308 NGNKHCSWCHYLSCVPTSRWDC 326
           NGN HC WCHYL CVPTS W C
Sbjct: 322 NGNDHCRWCHYLRCVPTSSWRC 343

BLAST of PI0006864 vs. TAIR 10
Match: AT2G29050.1 (RHOMBOID-like 1 )

HSP 1 Score: 406.8 bits (1044), Expect = 1.7e-113
Identity = 196/296 (66.22%), Postives = 241/296 (81.42%), Query Frame = 0

Query: 35  KQWTPWLVPMFVVANIAMFIVVMYVNNCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLGP 94
           K W PWLVP  VVANIA+F + M++NNCP ++      C+A FLGRF+FQP++ENPLLGP
Sbjct: 52  KLWFPWLVPAIVVANIALFAISMFINNCPKNSA----YCLARFLGRFAFQPMKENPLLGP 111

Query: 95  SSNTLVKLGALKWNKVVHEHQGWRLFSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFVR 154
           SS TL K+GAL  + VVH+H+ WRLF+CIWLHAG+ H+LANMLSL+ IGIRLEQ+FGFVR
Sbjct: 112 SSLTLEKMGALDVSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFVR 171

Query: 155 IGLIYLVAGVGGSVMSSLFIQNNISVGASGALFGLLGAMLSELLTNWTIYTNKAAALFTL 214
           IGL+Y+++G GGS++SSLF +  ISVGASGALFGLLGAMLSELLTNWTIY NK AAL TL
Sbjct: 172 IGLLYMISGFGGSLLSSLFNRAGISVGASGALFGLLGAMLSELLTNWTIYANKFAALLTL 231

Query: 215 IVIVVINLAVGILPHVDNFAHIGGFLTGFLLGFVLLVRPQFKWTERHHLP-----PSAQR 274
           I I+ INLAVGILPHVDNFAH+GGF +GFLLGFV L+RPQ+ +  + + P     PSA+ 
Sbjct: 232 IFIIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIRPQYGYFNQRNNPRGYAAPSAK- 291

Query: 275 VPKYKTYQYILWLAAAILLVAGFTLGLVMLFRGENGNKHCSWCHYLSCVPTSRWDC 326
             K+K YQY+LW+ + +LL+AG+T GLV+L RG + NKHCSWCHYLSC+PTS W C
Sbjct: 292 -SKHKPYQYVLWITSLVLLIAGYTAGLVVLLRGTDLNKHCSWCHYLSCIPTSLWSC 341

BLAST of PI0006864 vs. TAIR 10
Match: AT1G12750.1 (RHOMBOID-like protein 6 )

HSP 1 Score: 391.3 bits (1004), Expect = 7.4e-109
Identity = 185/293 (63.14%), Postives = 227/293 (77.47%), Query Frame = 0

Query: 34  EKQWTPWLVPMFVVANIAMFIVVMYVNNCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLG 93
           + QWT WL P  VVAN+++FIVVMY N+CP +  G    CVA  L RFSFQP+RENP LG
Sbjct: 15  DTQWTAWLTPTIVVANVSIFIVVMYTNDCPKTTTGANGDCVAKLLRRFSFQPLRENPFLG 74

Query: 94  PSSNTLVKLGALKWNKVVHEHQGWRLFSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFV 153
           PSS+TL KLGAL W KVV  ++ WRL + +WLHAGIIHL+ NM  +++ GIRLEQQFGF+
Sbjct: 75  PSSSTLEKLGALDWKKVVQGNEKWRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGFI 134

Query: 154 RIGLIYLVAGVGGSVMSSLFIQNNISVGASGALFGLLGAMLSELLTNWTIYTNKAAALFT 213
           RIGLIYL++G GGS++S+LF+Q +ISVGASGAL GL+GAMLSELLTNWTIY +K  AL +
Sbjct: 135 RIGLIYLISGFGGSILSALFLQKSISVGASGALLGLMGAMLSELLTNWTIYKSKLCALLS 194

Query: 214 LIVIVVINLAVGILPHVDNFAHIGGFLTGFLLGFVLLVRPQFKWTE-RHHLPPSAQRVPK 273
            + I+ INLA+G+LP VDNFAHIGG LTGF LGF+LL++PQ  W E R+     A+   K
Sbjct: 195 FLFIIAINLAIGLLPWVDNFAHIGGLLTGFCLGFILLMQPQSGWEEFRNSSQYGARARSK 254

Query: 274 YKTYQYILWLAAAILLVAGFTLGLVMLFRGENGNKHCSWCHYLSCVPTSRWDC 326
           Y   QY+L+  AA+L+VAG T+GLVMLF GENGNKHC WCH L C PTS+W C
Sbjct: 255 YNPCQYVLFFVAAVLVVAGLTVGLVMLFDGENGNKHCKWCHRLDCYPTSKWSC 307

BLAST of PI0006864 vs. TAIR 10
Match: AT1G12750.2 (RHOMBOID-like protein 6 )

HSP 1 Score: 391.3 bits (1004), Expect = 7.4e-109
Identity = 185/293 (63.14%), Postives = 227/293 (77.47%), Query Frame = 0

Query: 34  EKQWTPWLVPMFVVANIAMFIVVMYVNNCPDSNRGVGDKCVAGFLGRFSFQPIRENPLLG 93
           + QWT WL P  VVAN+++FIVVMY N+CP +  G    CVA  L RFSFQP+RENP LG
Sbjct: 15  DTQWTAWLTPTIVVANVSIFIVVMYTNDCPKTTTGANGDCVAKLLRRFSFQPLRENPFLG 74

Query: 94  PSSNTLVKLGALKWNKVVHEHQGWRLFSCIWLHAGIIHLLANMLSLVLIGIRLEQQFGFV 153
           PSS+TL KLGAL W KVV  ++ WRL + +WLHAGIIHL+ NM  +++ GIRLEQQFGF+
Sbjct: 75  PSSSTLEKLGALDWKKVVQGNEKWRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGFI 134

Query: 154 RIGLIYLVAGVGGSVMSSLFIQNNISVGASGALFGLLGAMLSELLTNWTIYTNKAAALFT 213
           RIGLIYL++G GGS++S+LF+Q +ISVGASGAL GL+GAMLSELLTNWTIY +K  AL +
Sbjct: 135 RIGLIYLISGFGGSILSALFLQKSISVGASGALLGLMGAMLSELLTNWTIYKSKLCALLS 194

Query: 214 LIVIVVINLAVGILPHVDNFAHIGGFLTGFLLGFVLLVRPQFKWTE-RHHLPPSAQRVPK 273
            + I+ INLA+G+LP VDNFAHIGG LTGF LGF+LL++PQ  W E R+     A+   K
Sbjct: 195 FLFIIAINLAIGLLPWVDNFAHIGGLLTGFCLGFILLMQPQSGWEEFRNSSQYGARARSK 254

Query: 274 YKTYQYILWLAAAILLVAGFTLGLVMLFRGENGNKHCSWCHYLSCVPTSRWDC 326
           Y   QY+L+  AA+L+VAG T+GLVMLF GENGNKHC WCH L C PTS+W C
Sbjct: 255 YNPCQYVLFFVAAVLVVAGLTVGLVMLFDGENGNKHCKWCHRLDCYPTSKWSC 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9CAN17.2e-12567.18RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1[more]
Q9LYP13.6e-12466.46RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1[more]
Q0WQX72.4e-11266.22RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1[more]
Q8VZ481.0e-10763.14RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL6 P... [more]
F4JBM41.4e-10460.82RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7TMJ51.2e-18396.94RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44... [more]
A0A1S3AU271.2e-18396.94RHOMBOID-like protein OS=Cucumis melo OX=3656 GN=LOC103482712 PE=3 SV=1[more]
A0A0A0KMN41.0e-18296.94RHOMBOID-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G155500 PE=3 SV=1[more]
A0A6J1H4571.7e-17491.74RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111459437 PE=3 SV=1[more]
A0A6J1JZC34.0e-17190.52RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111491076 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008437215.12.5e-18396.94PREDICTED: RHOMBOID-like protein 2 [Cucumis melo] >KAA0042791.1 RHOMBOID-like pr... [more]
XP_004143929.12.1e-18296.94RHOMBOID-like protein 2 [Cucumis sativus] >KGN50139.1 hypothetical protein Csa_0... [more]
XP_038874691.18.3e-17994.80RHOMBOID-like protein 2 [Benincasa hispida][more]
XP_023533312.14.3e-17592.05RHOMBOID-like protein 2 [Cucurbita pepo subsp. pepo][more]
KAG7036061.19.5e-17592.05RHOMBOID-like protein 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT1G63120.15.1e-12667.18RHOMBOID-like 2 [more]
AT5G07250.12.5e-12566.46RHOMBOID-like protein 3 [more]
AT2G29050.11.7e-11366.22RHOMBOID-like 1 [more]
AT1G12750.17.4e-10963.14RHOMBOID-like protein 6 [more]
AT1G12750.27.4e-10963.14RHOMBOID-like protein 6 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022764Peptidase S54, rhomboid domainPFAMPF01694Rhomboidcoord: 111..252
e-value: 1.2E-41
score: 142.2
IPR035952Rhomboid-like superfamilyGENE3D1.20.1540.10coord: 73..254
e-value: 1.2E-39
score: 138.0
IPR035952Rhomboid-like superfamilySUPERFAMILY144091Rhomboid-likecoord: 36..256
IPR002610Peptidase S54, rhomboidPANTHERPTHR22936RHOMBOID-RELATEDcoord: 2..327
NoneNo IPR availablePANTHERPTHR22936:SF93RHOMBOID-LIKE PROTEINcoord: 2..327

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0006864.1PI0006864.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity