PI0006538 (gene) Melon (PI 482460) v1

Overview
NamePI0006538
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionReverse transcriptase
Locationchr06: 20787669 .. 20788354 (+)
RNA-Seq ExpressionPI0006538
SyntenyPI0006538
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGGCCGAAGAGGAGGAATCCCTGCCACGTGGGACAAACGTGAGCAAGAAGAGGTGGAGGAGATCGCCACCCTCTCTCCAAGAACATCCACGGTACGCTTGCTGGCCGTCGAAGACTCTTTGGGAGATTTACACGAAAAATTTGATAGAGTAATGGATACTTTGGAGACACTGACTCGAAGGATGGACGGACTACCGGCTCCAGTAAGAATTGAAGCCAACGTAAATAATGATCGGAACGACGGAAACAGAGGAGGCCGACGAGCTCGAAGGAATTTTAGAACTTTGCCCAATCAAAGGAATGATCAAAGAATGAGGCCAATGGAGAATCCGCTGCGATATGCCGACGATGATTCAATGGAAGAATATGGAGCTTGGCAAAATGGGCAAGAATGTGATTCTTCCAGTGGTGATGAGCAAGGAAATATTTGGAATGATAATGGAGATATTCGAATGAGACGAGTTTATAGAGGACGTGAAGCACGAAGGGAAGTTCATCATGACTATAAGATGAAAATTGATCTTCCAACCTATGATGGTAAGCGTGATATTGAATCTTTTTTGGATTGGATTAAAAATACTGAAAATTTCTTCAATTATATGGATACTCCTGATAGAAAGAAAGTACACTTGGTGGCCCTAAAGCTACGTGGAGGAGCTTCGACATGGTGGGATCAGCTTGA

mRNA sequence

ATGGCTGGCCGAAGAGGAGGAATCCCTGCCACGTGGGACAAACGTGAGCAAGAAGAGGTGGAGGAGATCGCCACCCTCTCTCCAAGAACATCCACGGTACGCTTGCTGGCCGTCGAAGACTCTTTGGGAGATTTACACGAAAAATTTGATAGAGTAATGGATACTTTGGAGACACTGACTCGAAGGATGGACGGACTACCGGCTCCAGTAAGAATTGAAGCCAACGTAAATAATGATCGGAACGACGGAAACAGAGGAGGCCGACGAGCTCGAAGGAATTTTAGAACTTTGCCCAATCAAAGGAATGATCAAAGAATGAGGCCAATGGAGAATCCGCTGCGATATGCCGACGATGATTCAATGGAAGAATATGGAGCTTGGCAAAATGGGCAAGAATGTGATTCTTCCAGTGGTGATGAGCAAGGAAATATTTGGAATGATAATGGAGATATTCGAATGAGACGAGTTTATAGAGGACGTGAAGCACGAAGGGAAGTTCATCATGACTATAAGATGAAAATTGATCTTCCAACCTATGATGAAAGTACACTTGGTGGCCCTAAAGCTACGTGGAGGAGCTTCGACATGGTGGGATCAGCTTGA

Coding sequence (CDS)

ATGGCTGGCCGAAGAGGAGGAATCCCTGCCACGTGGGACAAACGTGAGCAAGAAGAGGTGGAGGAGATCGCCACCCTCTCTCCAAGAACATCCACGGTACGCTTGCTGGCCGTCGAAGACTCTTTGGGAGATTTACACGAAAAATTTGATAGAGTAATGGATACTTTGGAGACACTGACTCGAAGGATGGACGGACTACCGGCTCCAGTAAGAATTGAAGCCAACGTAAATAATGATCGGAACGACGGAAACAGAGGAGGCCGACGAGCTCGAAGGAATTTTAGAACTTTGCCCAATCAAAGGAATGATCAAAGAATGAGGCCAATGGAGAATCCGCTGCGATATGCCGACGATGATTCAATGGAAGAATATGGAGCTTGGCAAAATGGGCAAGAATGTGATTCTTCCAGTGGTGATGAGCAAGGAAATATTTGGAATGATAATGGAGATATTCGAATGAGACGAGTTTATAGAGGACGTGAAGCACGAAGGGAAGTTCATCATGACTATAAGATGAAAATTGATCTTCCAACCTATGATGAAAGTACACTTGGTGGCCCTAAAGCTACGTGGAGGAGCTTCGACATGGTGGGATCAGCTTGA

Protein sequence

MAGRRGGIPATWDKREQEEVEEIATLSPRTSTVRLLAVEDSLGDLHEKFDRVMDTLETLTRRMDGLPAPVRIEANVNNDRNDGNRGGRRARRNFRTLPNQRNDQRMRPMENPLRYADDDSMEEYGAWQNGQECDSSSGDEQGNIWNDNGDIRMRRVYRGREARREVHHDYKMKIDLPTYDESTLGGPKATWRSFDMVGSA
Homology
BLAST of PI0006538 vs. ExPASy TrEMBL
Match: A0A5D3CJ99 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G00470 PE=4 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 1.1e-81
Identity = 158/179 (88.27%), Postives = 163/179 (91.06%), Query Frame = 0

Query: 1   MAGRRGGIPATWDKREQEEVEEIATLSPRTSTVRLLAVEDSLGDLHEKFDRVMDTLETLT 60
           MAGRR GIPAT D REQEEVEE A LSPRTSTV  LAVEDSLGDLHEKFDRVMDTLETLT
Sbjct: 1   MAGRREGIPATGDNREQEEVEENAALSPRTSTVCFLAVEDSLGDLHEKFDRVMDTLETLT 60

Query: 61  RRMDGLPAPVRIEANVNNDRNDGNRGGRRARRNFRTLPNQRNDQRMRPMENPLRYADDDS 120
           RRMDGLPA  RIEAN NNDRNDGNRGGRRARRNFR LPNQRNDQR RPMENPLRYAD+DS
Sbjct: 61  RRMDGLPALARIEANANNDRNDGNRGGRRARRNFRNLPNQRNDQRKRPMENPLRYADNDS 120

Query: 121 MEEYGAWQNGQECDSSSGDEQGNIWNDNGDIRMRRVYRGREARREVHHDYKMKIDLPTY 180
           MEEY AWQNGQECDSSS DEQGNIWNDN D++MR+VYRG EA+REVHHDYKMKIDLPTY
Sbjct: 121 MEEYEAWQNGQECDSSSSDEQGNIWNDNRDMQMRQVYRGHEAQREVHHDYKMKIDLPTY 179

BLAST of PI0006538 vs. ExPASy TrEMBL
Match: A0A5A7UQ86 (RNA-directed DNA polymerase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold280G001990 PE=4 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 5.3e-73
Identity = 143/180 (79.44%), Postives = 159/180 (88.33%), Query Frame = 0

Query: 1   MAGRRGGIPATWDKREQEEVEEIATLSPRTSTVRLLAVEDSLGDLHEKFDRVMDTLETLT 60
           MA RRGGIPA  D REQEEVE IATLSPRTSTVRLLAV+D LGDLHEKF+R+MD LETLT
Sbjct: 1   MADRRGGIPAAGDNREQEEVEAIATLSPRTSTVRLLAVKDFLGDLHEKFNRMMDNLETLT 60

Query: 61  RRMDGLPAPVRIEANVNNDRNDGNRGGRRARRNFRTLPNQRNDQRMRPMENPLRYADDDS 120
           +RMDGLPAP RIEAN+N DRN+ NRGGRRA+RNF  +PNQRNDQ  RPME PLRYADDDS
Sbjct: 61  QRMDGLPAPARIEANMNIDRNERNRGGRRAQRNFSNMPNQRNDQIRRPMEMPLRYADDDS 120

Query: 121 MEEYGAWQNGQECDSSSGDEQGNIWNDNGDIRMRRVYRGREARREVHHDYKMKIDLPTYD 180
           +E+  AWQN QE DSSSGDE+GNIW+DNGDI+MR+ YRG EARREVHHDYKMK+DLPTY+
Sbjct: 121 LEKGDAWQNRQEYDSSSGDERGNIWDDNGDIQMRQAYRGHEARREVHHDYKMKLDLPTYN 180

BLAST of PI0006538 vs. ExPASy TrEMBL
Match: A0A5D3DKV9 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G00260 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.4e-57
Identity = 120/180 (66.67%), Postives = 136/180 (75.56%), Query Frame = 0

Query: 1   MAGRRGGIPATWDKREQEEVEEIATLSPRTSTVRLLAVEDSLGDLHEKFDRVMDTLETLT 60
           MAG+RG IPA WD REQ+E EEI  LSPRTSTV LLAVEDSLGDLH+KFD++MD+LETL 
Sbjct: 1   MAGQRGAIPAAWDNREQQEAEEITVLSPRTSTVHLLAVEDSLGDLHDKFDKMMDSLETLN 60

Query: 61  RRMDGLPAPVRIEANVNNDRNDGNRGGRRARRNFRTLPNQRNDQRMRPMENPLRYADDDS 120
           +RMDGL AP RIEANV NDRNDGN+GGR+ARRN R LPNQ+ND+  RP E PL       
Sbjct: 61  QRMDGLLAPARIEANVVNDRNDGNKGGRQARRNHRNLPNQKNDRIRRPNEVPL------- 120

Query: 121 MEEYGAWQNGQECDSSSGDEQGNIWNDNGDIRMRRVYRGREARREVHHDYKMKIDLPTYD 180
                            GDEQGNIWNDNGD +MR+ YRG+EARREVHHDYKMKIDLP ++
Sbjct: 121 -----------------GDEQGNIWNDNGDFQMRQAYRGQEARREVHHDYKMKIDLPVHN 156

BLAST of PI0006538 vs. ExPASy TrEMBL
Match: A0A5A7UR03 (2-methoxy-6-polyprenyl-1,4-benzoquinol methylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold486G00180 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 2.0e-56
Identity = 122/145 (84.14%), Postives = 127/145 (87.59%), Query Frame = 0

Query: 9   PATWDKREQEEVEEIATLSPRTSTVRLLAVEDSLGDLHEKFDRVMDTLETLTRRMDGLPA 68
           P    + EQE+VEEI TLSPRTSTV LLA EDSLGDLHEKFDRVMDTLETLTRRMD L A
Sbjct: 294 PCHGGQPEQEKVEEIDTLSPRTSTVHLLAFEDSLGDLHEKFDRVMDTLETLTRRMDRLLA 353

Query: 69  PVRIEANVNNDR--NDGNRGGRRARRNFRTLPNQRNDQRMRPMENPLRYADDDSMEEYGA 128
           P RIEANVNN+R  NDGNRGGRRARRNFR LPNQR+DQR RPMENPLRYADD+SMEEY A
Sbjct: 354 PARIEANVNNNRNKNDGNRGGRRARRNFRNLPNQRHDQRRRPMENPLRYADDNSMEEYEA 413

Query: 129 WQNGQECDSSSGDEQGNIWNDNGDI 152
            QNGQECDSSS DEQGNIWNDNGDI
Sbjct: 414 RQNGQECDSSSSDEQGNIWNDNGDI 438

BLAST of PI0006538 vs. ExPASy TrEMBL
Match: A0A5D3DBZ2 (2-methoxy-6-polyprenyl-1,4-benzoquinol methylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold778G00120 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 2.0e-56
Identity = 122/145 (84.14%), Postives = 127/145 (87.59%), Query Frame = 0

Query: 9   PATWDKREQEEVEEIATLSPRTSTVRLLAVEDSLGDLHEKFDRVMDTLETLTRRMDGLPA 68
           P    + EQE+VEEI TLSPRTSTV LLA EDSLGDLHEKFDRVMDTLETLTRRMD L A
Sbjct: 205 PCHGGQPEQEKVEEIDTLSPRTSTVHLLAFEDSLGDLHEKFDRVMDTLETLTRRMDRLLA 264

Query: 69  PVRIEANVNNDR--NDGNRGGRRARRNFRTLPNQRNDQRMRPMENPLRYADDDSMEEYGA 128
           P RIEANVNN+R  NDGNRGGRRARRNFR LPNQR+DQR RPMENPLRYADD+SMEEY A
Sbjct: 265 PARIEANVNNNRNKNDGNRGGRRARRNFRNLPNQRHDQRRRPMENPLRYADDNSMEEYEA 324

Query: 129 WQNGQECDSSSGDEQGNIWNDNGDI 152
            QNGQECDSSS DEQGNIWNDNGDI
Sbjct: 325 RQNGQECDSSSSDEQGNIWNDNGDI 349

BLAST of PI0006538 vs. NCBI nr
Match: TYK11204.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 312.8 bits (800), Expect = 2.2e-81
Identity = 158/179 (88.27%), Postives = 163/179 (91.06%), Query Frame = 0

Query: 1   MAGRRGGIPATWDKREQEEVEEIATLSPRTSTVRLLAVEDSLGDLHEKFDRVMDTLETLT 60
           MAGRR GIPAT D REQEEVEE A LSPRTSTV  LAVEDSLGDLHEKFDRVMDTLETLT
Sbjct: 1   MAGRREGIPATGDNREQEEVEENAALSPRTSTVCFLAVEDSLGDLHEKFDRVMDTLETLT 60

Query: 61  RRMDGLPAPVRIEANVNNDRNDGNRGGRRARRNFRTLPNQRNDQRMRPMENPLRYADDDS 120
           RRMDGLPA  RIEAN NNDRNDGNRGGRRARRNFR LPNQRNDQR RPMENPLRYAD+DS
Sbjct: 61  RRMDGLPALARIEANANNDRNDGNRGGRRARRNFRNLPNQRNDQRKRPMENPLRYADNDS 120

Query: 121 MEEYGAWQNGQECDSSSGDEQGNIWNDNGDIRMRRVYRGREARREVHHDYKMKIDLPTY 180
           MEEY AWQNGQECDSSS DEQGNIWNDN D++MR+VYRG EA+REVHHDYKMKIDLPTY
Sbjct: 121 MEEYEAWQNGQECDSSSSDEQGNIWNDNRDMQMRQVYRGHEAQREVHHDYKMKIDLPTY 179

BLAST of PI0006538 vs. NCBI nr
Match: KAA0057344.1 (RNA-directed DNA polymerase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 283.9 bits (725), Expect = 1.1e-72
Identity = 143/180 (79.44%), Postives = 159/180 (88.33%), Query Frame = 0

Query: 1   MAGRRGGIPATWDKREQEEVEEIATLSPRTSTVRLLAVEDSLGDLHEKFDRVMDTLETLT 60
           MA RRGGIPA  D REQEEVE IATLSPRTSTVRLLAV+D LGDLHEKF+R+MD LETLT
Sbjct: 1   MADRRGGIPAAGDNREQEEVEAIATLSPRTSTVRLLAVKDFLGDLHEKFNRMMDNLETLT 60

Query: 61  RRMDGLPAPVRIEANVNNDRNDGNRGGRRARRNFRTLPNQRNDQRMRPMENPLRYADDDS 120
           +RMDGLPAP RIEAN+N DRN+ NRGGRRA+RNF  +PNQRNDQ  RPME PLRYADDDS
Sbjct: 61  QRMDGLPAPARIEANMNIDRNERNRGGRRAQRNFSNMPNQRNDQIRRPMEMPLRYADDDS 120

Query: 121 MEEYGAWQNGQECDSSSGDEQGNIWNDNGDIRMRRVYRGREARREVHHDYKMKIDLPTYD 180
           +E+  AWQN QE DSSSGDE+GNIW+DNGDI+MR+ YRG EARREVHHDYKMK+DLPTY+
Sbjct: 121 LEKGDAWQNRQEYDSSSGDERGNIWDDNGDIQMRQAYRGHEARREVHHDYKMKLDLPTYN 180

BLAST of PI0006538 vs. NCBI nr
Match: KAA0056581.1 (reverse transcriptase [Cucumis melo var. makuwa] >TYK24263.1 reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 232.6 bits (592), Expect = 2.9e-57
Identity = 120/180 (66.67%), Postives = 136/180 (75.56%), Query Frame = 0

Query: 1   MAGRRGGIPATWDKREQEEVEEIATLSPRTSTVRLLAVEDSLGDLHEKFDRVMDTLETLT 60
           MAG+RG IPA WD REQ+E EEI  LSPRTSTV LLAVEDSLGDLH+KFD++MD+LETL 
Sbjct: 1   MAGQRGAIPAAWDNREQQEAEEITVLSPRTSTVHLLAVEDSLGDLHDKFDKMMDSLETLN 60

Query: 61  RRMDGLPAPVRIEANVNNDRNDGNRGGRRARRNFRTLPNQRNDQRMRPMENPLRYADDDS 120
           +RMDGL AP RIEANV NDRNDGN+GGR+ARRN R LPNQ+ND+  RP E PL       
Sbjct: 61  QRMDGLLAPARIEANVVNDRNDGNKGGRQARRNHRNLPNQKNDRIRRPNEVPL------- 120

Query: 121 MEEYGAWQNGQECDSSSGDEQGNIWNDNGDIRMRRVYRGREARREVHHDYKMKIDLPTYD 180
                            GDEQGNIWNDNGD +MR+ YRG+EARREVHHDYKMKIDLP ++
Sbjct: 121 -----------------GDEQGNIWNDNGDFQMRQAYRGQEARREVHHDYKMKIDLPVHN 156

BLAST of PI0006538 vs. NCBI nr
Match: TYK21018.1 (2-methoxy-6-polyprenyl-1,4-benzoquinol methylase [Cucumis melo var. makuwa])

HSP 1 Score: 228.8 bits (582), Expect = 4.2e-56
Identity = 122/145 (84.14%), Postives = 127/145 (87.59%), Query Frame = 0

Query: 9   PATWDKREQEEVEEIATLSPRTSTVRLLAVEDSLGDLHEKFDRVMDTLETLTRRMDGLPA 68
           P    + EQE+VEEI TLSPRTSTV LLA EDSLGDLHEKFDRVMDTLETLTRRMD L A
Sbjct: 205 PCHGGQPEQEKVEEIDTLSPRTSTVHLLAFEDSLGDLHEKFDRVMDTLETLTRRMDRLLA 264

Query: 69  PVRIEANVNNDR--NDGNRGGRRARRNFRTLPNQRNDQRMRPMENPLRYADDDSMEEYGA 128
           P RIEANVNN+R  NDGNRGGRRARRNFR LPNQR+DQR RPMENPLRYADD+SMEEY A
Sbjct: 265 PARIEANVNNNRNKNDGNRGGRRARRNFRNLPNQRHDQRRRPMENPLRYADDNSMEEYEA 324

Query: 129 WQNGQECDSSSGDEQGNIWNDNGDI 152
            QNGQECDSSS DEQGNIWNDNGDI
Sbjct: 325 RQNGQECDSSSSDEQGNIWNDNGDI 349

BLAST of PI0006538 vs. NCBI nr
Match: KAA0056746.1 (2-methoxy-6-polyprenyl-1,4-benzoquinol methylase [Cucumis melo var. makuwa])

HSP 1 Score: 228.8 bits (582), Expect = 4.2e-56
Identity = 122/145 (84.14%), Postives = 127/145 (87.59%), Query Frame = 0

Query: 9   PATWDKREQEEVEEIATLSPRTSTVRLLAVEDSLGDLHEKFDRVMDTLETLTRRMDGLPA 68
           P    + EQE+VEEI TLSPRTSTV LLA EDSLGDLHEKFDRVMDTLETLTRRMD L A
Sbjct: 294 PCHGGQPEQEKVEEIDTLSPRTSTVHLLAFEDSLGDLHEKFDRVMDTLETLTRRMDRLLA 353

Query: 69  PVRIEANVNNDR--NDGNRGGRRARRNFRTLPNQRNDQRMRPMENPLRYADDDSMEEYGA 128
           P RIEANVNN+R  NDGNRGGRRARRNFR LPNQR+DQR RPMENPLRYADD+SMEEY A
Sbjct: 354 PARIEANVNNNRNKNDGNRGGRRARRNFRNLPNQRHDQRRRPMENPLRYADDNSMEEYEA 413

Query: 129 WQNGQECDSSSGDEQGNIWNDNGDI 152
            QNGQECDSSS DEQGNIWNDNGDI
Sbjct: 414 RQNGQECDSSSSDEQGNIWNDNGDI 438

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3CJ991.1e-8188.27Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold22... [more]
A0A5A7UQ865.3e-7379.44RNA-directed DNA polymerase-like protein OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5D3DKV91.4e-5766.67Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold20... [more]
A0A5A7UR032.0e-5684.142-methoxy-6-polyprenyl-1,4-benzoquinol methylase OS=Cucumis melo var. makuwa OX=... [more]
A0A5D3DBZ22.0e-5684.142-methoxy-6-polyprenyl-1,4-benzoquinol methylase OS=Cucumis melo var. makuwa OX=... [more]
Match NameE-valueIdentityDescription
TYK11204.12.2e-8188.27reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0057344.11.1e-7279.44RNA-directed DNA polymerase-like protein [Cucumis melo var. makuwa][more]
KAA0056581.12.9e-5766.67reverse transcriptase [Cucumis melo var. makuwa] >TYK24263.1 reverse transcripta... [more]
TYK21018.14.2e-5684.142-methoxy-6-polyprenyl-1,4-benzoquinol methylase [Cucumis melo var. makuwa][more]
KAA0056746.14.2e-5684.142-methoxy-6-polyprenyl-1,4-benzoquinol methylase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 75..94
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 9..23

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0006538.1PI0006538.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0016310 phosphorylation
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
molecular_function GO:0004386 helicase activity
molecular_function GO:0016301 kinase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003964 RNA-directed DNA polymerase activity
molecular_function GO:0008270 zinc ion binding