PI0006227 (gene) Melon (PI 482460) v1

BLAST of PI0006227 vs. ExPASy Swiss-Prot
Match: Q9LZS7 (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 5.4e-80
Identity = 171/355 (48.17%), Postives = 221/355 (62.25%), Query Frame = 0

Query: 1   MDSQ-KLLFSLFIIL---LLSDQGSG----GHNDLLREFQPKKLYVFGDSYVDTGNIGIS 60
           MDS  KL F LFI L   LL  + +G      N  L  F+P KL+VFGDSY DTGNI  +
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLFGEINGVEGSNQNHHLYPFRPTKLFVFGDSYADTGNIKKA 60

Query: 61  NYSSRKFPYGITFPGKPSGRFSDGRVLTDYVVSY-----PISSLQRFKRSKERKNINGVN 120
             SS KFPYGITFPGKP+GRFSDGRV TD++  +     PI    +    K+R    G+N
Sbjct: 61  FSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRLQY-GMN 120

Query: 121 FAFGGTGVFNTSVSFPNMTTQIDLFHNFIDETDI------QSSIALVSVSGNDYSFYLAH 180
           FA+GGTGVFNT    PNMTTQID+F N +   DI       SS+ALVSV+GNDYS ++A 
Sbjct: 121 FAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVAGNDYSNFIAL 180

Query: 181 NGSLEGLKGFMISVVDQIIWNLKRILSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSNI 240
           N        F+  VVDQ   NL+RI +LGVKK+ V  L PLGCLP FT  +SF +CN   
Sbjct: 181 NRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNETQ 240

Query: 241 NSLVTFHNLLLNQSISKLNNNNETTTKNNINFFILDMYDSFMSILFGNPKQGGEKPFD-- 300
           N+LV  HN LL Q ++KLNN  + +T     F ILD+Y++F+++        G   F+  
Sbjct: 241 NALVNLHNNLLQQVVAKLNNETKQST-----FIILDLYNAFLTVFKNKGSNPGSTRFESP 300

Query: 301 ----------EFSCGSVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAFTTLQ 325
                     E++CGSVDEKG KK+ +C++PK+AFFWD LHPT++GW + ++ L+
Sbjct: 301 LKPCCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWRSVYSVLR 349

BLAST of PI0006227 vs. ExPASy Swiss-Prot
Match: Q9SF94 (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 3.2e-72
Identity = 159/359 (44.29%), Postives = 213/359 (59.33%), Query Frame = 0

Query: 1   MDSQKLLFSLFII----LLLSDQGSGGHNDLLREFQPKKLYVFGDSYVDTGNIGISNYSS 60
           M+  KLL SLF+       L  +     +  L+  +P +L+VFGDSY DTGNI  S   S
Sbjct: 1   MELPKLLISLFLFSFSSFFLGAESDYPQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSDS 60

Query: 61  RKFPYGITFPGKPSGRFSDGRVLTDYVVSY-----PISSLQRFKRSKERKNINGVNFAFG 120
            K PYGITFP KPSGRFSDGRV TD++  Y     PI    +    KER  + G+N+A+G
Sbjct: 61  WKIPYGITFPQKPSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERL-LYGMNYAYG 120

Query: 121 GTGVFNTSVS-FPNMTTQIDLFH------NFIDETDIQSSIALVSVSGNDYSFYLAHNGS 180
           GTGVF T  +  PNMTTQID F       N    +D+ SS+ALVSV+GNDY+ +LA    
Sbjct: 121 GTGVFKTKDNPLPNMTTQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRP 180

Query: 181 LEGLKGFMISVVDQIIWNLKRILSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSNINSL 240
           L  L  FM  VVDQI  N  RI  LGV K+V+  + PLGCLP  T  +SF +CN+  N+ 
Sbjct: 181 LTELPAFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNAS 240

Query: 241 VTFHNLLLNQSISKLNNNNETTTKNNINFFILDMYDSFMSIL-----------FGNPKQ- 300
              HN LL+++I++LNN  + +T     F +LD Y++F+++            FGNP + 
Sbjct: 241 TNLHNYLLHKAIARLNNETKPST-----FVVLDHYNAFLTVFKNKGPEPGVSRFGNPLKP 300

Query: 301 ---GGEKPFDEFSCGSVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAFTTLQFILK 329
              G    +D   C +VDEKG KK+ +CEDPK+AFFWD  HP+++GW + ++ L   LK
Sbjct: 301 CCVGVNSSYD---CSNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVYSVLHKHLK 350

BLAST of PI0006227 vs. ExPASy Swiss-Prot
Match: Q9LZS9 (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 224.2 bits (570), Expect = 2.2e-57
Identity = 135/333 (40.54%), Postives = 196/333 (58.86%), Query Frame = 0

Query: 5   KLLFSLFIILLLSDQGSGGHNDLLREFQPKKLYVFGDSYVDTGNIGISNYSSRKFPYGIT 64
           KL FSL   L     G+ G N   +  +  KL+VFG+SY DTGN+      S K PYGIT
Sbjct: 6   KLFFSL--SLFFGINGAVGSNQPSQANKNPKLFVFGNSYADTGNMK-PTALSWKLPYGIT 65

Query: 65  FPGKPSGRFSDGRVLTDYVVSYPISSLQRFKRSKERKNI---NGVNFAFGGTGVFNTSVS 124
           FPGKPSGR+SDG   TD++     + L    R+  +K +    G+NFAFGG+ VF++ V 
Sbjct: 66  FPGKPSGRYSDGLTATDFLAKQLGAKLPYLWRTHGKKKVKLNRGMNFAFGGSEVFDSPVD 125

Query: 125 -FPNMTTQIDLFHN-------FIDETDIQSSIALVSVSGNDYSFYLAHNGSLEGLKGFMI 184
             PN++TQ+    N       +  + D+ SS AL+S SG DY  ++  N ++     F+ 
Sbjct: 126 RSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGFIDQNPNMAAYPAFVE 185

Query: 185 SVVDQIIWNLKRILSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSNINSLVTFHNLLLN 244
            +V+ I ++L  +  L  K + VT L PLGCLP  T +SSF  CN + + LV  HN  L 
Sbjct: 186 FIVEDIQYSLGIMNGLKFKNIAVTSLHPLGCLPRVTVASSFRSCNESYSDLVRLHNESLK 245

Query: 245 QSISKLNNNNETTTKNNINFFILDMYDSFMSIL--FGNPK-QGGEKPFDEFSCGSVDEKG 304
           ++++KLN  ++  TK +  F I+D++ +FM+IL   GN + +   KP  E  C  +D KG
Sbjct: 246 KAVAKLNKEDKFRTKGD-RFVIVDLHKAFMTILEKKGNKRFKSPLKPCCEGDCARMDMKG 305

Query: 305 NKKFSLCEDPKSAFFWDSLHPTQQGWFAAFTTL 324
            KK++LC DPKSAFFWD ++PTQ+GW + ++ L
Sbjct: 306 AKKYTLCNDPKSAFFWDEINPTQEGWRSIYSLL 334

BLAST of PI0006227 vs. ExPASy Swiss-Prot
Match: Q9LZS8 (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.8e-54
Identity = 121/314 (38.54%), Postives = 187/314 (59.55%), Query Frame = 0

Query: 23  GHNDLLREFQPKKLYVFGDSYVDTGNIGISNYSSRKFPYGITFPGKPSGRFSDGRVLTDY 82
           G N LLR  +  KL+VFGDSY DTGN    +  +   PYGITFPGKPSGR+ DG + TD+
Sbjct: 2   GSNQLLRPRKVPKLFVFGDSYADTGNTK-RDTEAWAIPYGITFPGKPSGRYCDGLIATDF 61

Query: 83  ---VVSYPISSLQRFKRSKERKNINGVNFAFGGTGVFNTSVS--FPNMTTQIDLFHNFID 142
              V+      L R    +++    G+NFAFGG+ + ++S +  FPN+T Q++   + + 
Sbjct: 62  LEKVLGAESPYLYR-THGRDKGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVL 121

Query: 143 ETDI-----QSSIALVSVSGNDYSFYLAHNGSLEGLKGFMISVVDQIIWNLKRILSLGVK 202
              +      S ++L+S +G DY +Y+  N    GLK  +  VVD +  N+  +  L  K
Sbjct: 122 AGRVYGDITPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFK 181

Query: 203 KVVVTGLAPLGCLPHFTNSSSFTQCNSNINSLVTFHNLLLNQSISKLNNNNETTTKNNIN 262
           K+ VT L P+GCLP +T++SSF  CN + ++LV  HN LL + ++KLN  +    K   +
Sbjct: 182 KIAVTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQ-H 241

Query: 263 FFILDMYDSFMSILFGNPKQGGEKPFD---EFSCGSVDEKGNKKFSLCEDPKSAFFWDSL 322
           FFI+D++++FM+++     +  + P     E  CG   + G K ++LC+DPKS FFWD++
Sbjct: 242 FFIIDIHNAFMTVMKNKGSKRFKNPMKSCCEGYCGRSSD-GGKLYTLCDDPKSFFFWDAV 301

Query: 323 HPTQQGWFAAFTTL 324
           HPTQ+GW + ++ L
Sbjct: 302 HPTQEGWRSIYSVL 311

BLAST of PI0006227 vs. ExPASy Swiss-Prot
Match: Q8RWJ4 (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 201.8 bits (512), Expect = 1.2e-50
Identity = 130/316 (41.14%), Postives = 171/316 (54.11%), Query Frame = 0

Query: 31  FQPKKLYVFGDSYVDTGNIGISNYSSRKFPYGITFPGKPSGRFSDGRVLTDYVVSY---- 90
           F+P KL+VFGDSY DTGN       S +FP GITFPG P+GRFSDGRV TDY+  Y    
Sbjct: 43  FKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVR 102

Query: 91  -PISSLQRFKRSKERKNI-NGVNFAFGGTGVFNTSVSF-PNMTTQID-----LFHNFIDE 150
            PI + +  K  + R  +  G+NFA+GG G F T     P  + QID     L  N    
Sbjct: 103 TPI-TYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRNVYSP 162

Query: 151 TDIQSSIALVSVSGNDYSFYLAHNGSLEGLKGFMISVVDQIIWNLKRILSLGVKKVVVTG 210
            D+ SS+A  S+ GNDY  Y   NGS EG       VV QI+ ++KRI  LGV+KV+V  
Sbjct: 163 ADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVAL 222

Query: 211 LAPLGCLPHFTNSSSFTQCNSNINSLVTFHNLLLNQSISKLNNNNETTTKNNINFFILDM 270
             P  CLP          C++N  S    HN LL + + KL  N++    N+ +F  LD+
Sbjct: 223 SPPQKCLPKLVTPKG---CDTNDTS-TYLHNSLLRKGLIKL--NDKEINNNDKSFMTLDL 282

Query: 271 YDSFMSILFGNPKQGGEKPF-DEFS----------CGSVDEKGNKKFSLCEDPKSAFFWD 324
           Y++F++I F N    G   F D F           CG     G K ++LC+DPKS FFWD
Sbjct: 283 YNAFVTI-FKNKGVSGVSTFPDPFKACCATKRGTFCGDRSLSGKKLYTLCDDPKSFFFWD 342

BLAST of PI0006227 vs. ExPASy TrEMBL
Match: A0A1S3CHQ7 (GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500888 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.0e-162
Identity = 298/350 (85.14%), Postives = 309/350 (88.29%), Query Frame = 0

Query: 1   MDSQKLLFSLFIILLLSDQGSGGHNDLLREFQPKKLYVFGDSYVDTGNIGISNYSSRKFP 60
           MDSQKLLFSLF ILLLS+QGS GHNDL+REF+PKKLYVFGDSYVDTGNIGISNYSSRK+P
Sbjct: 1   MDSQKLLFSLFTILLLSEQGSAGHNDLIREFRPKKLYVFGDSYVDTGNIGISNYSSRKYP 60

Query: 61  YGITFPGKPSGRFSDGRVLTDYVVSY-----PISSLQRFKRSKER----KNINGVNFAFG 120
           YGITFPGKPSGRFSDGRVLTDYV  Y     PI     FK  K R    KNINGVNFA+G
Sbjct: 61  YGITFPGKPSGRFSDGRVLTDYVARYLGLKPPIPFRVLFKDLKRRQGRKKNINGVNFAYG 120

Query: 121 GTGVFNTSVSFPNMTTQIDLFHNFIDETDIQSSIALVSVSGNDYSFYLAHNGSLEGLKGF 180
           GTGVFNTSVSFPNMTTQIDLFH FIDETDIQSSIALVSVSGNDYSFYLA NGSL+G KGF
Sbjct: 121 GTGVFNTSVSFPNMTTQIDLFHKFIDETDIQSSIALVSVSGNDYSFYLARNGSLQGFKGF 180

Query: 181 MISVVDQIIWNLKRILSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSNINSLVTFHNLL 240
           MISVVDQIIWNLKRI SLGVKKV+VTGLAPLGCLPHFTNSSSFTQCNS+INSLVTFHNLL
Sbjct: 181 MISVVDQIIWNLKRIHSLGVKKVIVTGLAPLGCLPHFTNSSSFTQCNSDINSLVTFHNLL 240

Query: 241 LNQSISKL-NNNNETTTKNNINFFILDMYDSFMSILFGNPKQGGEKPF----------DE 300
           LNQSISKL NNNNETTTKNN NFFILDMY SFMSI+FGNPKQGG+KP           D 
Sbjct: 241 LNQSISKLNNNNNETTTKNNTNFFILDMYASFMSIIFGNPKQGGKKPLLLKPCCFGVSDA 300

Query: 301 FSCGSVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAFTTLQFILKNL 331
           FSCGSVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAF+ LQFILKNL
Sbjct: 301 FSCGSVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAFSALQFILKNL 350

BLAST of PI0006227 vs. ExPASy TrEMBL
Match: A0A0A0KFB6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G452050 PE=3 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 8.5e-161
Identity = 293/348 (84.20%), Postives = 306/348 (87.93%), Query Frame = 0

Query: 3   SQKLLFSLFIILLLSDQGSGGHND-LLREFQPKKLYVFGDSYVDTGNIGISNYSSRKFPY 62
           SQKLLFSLF + LLS+QGSGGHND LLREFQPKKLYVFGDSYVDTGNIGISN+SSRKFPY
Sbjct: 4   SQKLLFSLFTVFLLSEQGSGGHNDNLLREFQPKKLYVFGDSYVDTGNIGISNFSSRKFPY 63

Query: 63  GITFPGKPSGRFSDGRVLTDYVVSY-------PISSLQRFKR--SKERKNINGVNFAFGG 122
           GITFPGKPSGRFSDGRVLTDYV  Y       P    +  KR   ++ KNINGVNFA+GG
Sbjct: 64  GITFPGKPSGRFSDGRVLTDYVARYLGLKPPIPFRVFKDLKRRQGRKNKNINGVNFAYGG 123

Query: 123 TGVFNTSVSFPNMTTQIDLFHNFIDETDIQSSIALVSVSGNDYSFYLAHNGSLEGLKGFM 182
           TGVFNTSVSFPNMTTQIDLFH FIDETDIQSSIALVSVSGNDYSFYLA NGSL+G KGFM
Sbjct: 124 TGVFNTSVSFPNMTTQIDLFHKFIDETDIQSSIALVSVSGNDYSFYLARNGSLQGFKGFM 183

Query: 183 ISVVDQIIWNLKRILSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSNINSLVTFHNLLL 242
           ISVVDQI+WNLKR+ SLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNS+INSLVTFHNLLL
Sbjct: 184 ISVVDQIMWNLKRVHSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSDINSLVTFHNLLL 243

Query: 243 NQSISKLNNNNETTTKNNINFFILDMYDSFMSILFGNPKQGGEKPF----------DEFS 302
           NQSISKLNNNNETTTKNN NFFILDMY SFMSI+FGNPKQGGEKP           D FS
Sbjct: 244 NQSISKLNNNNETTTKNNTNFFILDMYASFMSIIFGNPKQGGEKPLLLKPCCFGVSDGFS 303

Query: 303 CGSVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAFTTLQFILKNL 331
           CGSVDEKGNKKFSLCEDPKSAFFWDS HPTQQGWFAAF+TLQ ILKNL
Sbjct: 304 CGSVDEKGNKKFSLCEDPKSAFFWDSFHPTQQGWFAAFSTLQSILKNL 351

BLAST of PI0006227 vs. ExPASy TrEMBL
Match: A0A6J1BU10 (GDSL esterase/lipase At5g03610-like OS=Momordica charantia OX=3673 GN=LOC111005765 PE=3 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 5.8e-93
Identity = 195/359 (54.32%), Postives = 242/359 (67.41%), Query Frame = 0

Query: 1   MDSQKLLFSLFIIL----LLSDQGSGGHNDLLREFQPKKLYVFGDSYVDTGNIGISNYSS 60
           MDS+KLLFSL   L    LL  QGS  ++      +P +L+VFGDSYVDTGNI ++  S+
Sbjct: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRHFHLRPTRLFVFGDSYVDTGNINVNTSSA 60

Query: 61  RKFPYGITFPGKPSGRFSDGRVLTDYVVSY-------PISSLQRFKRSKERKNINGVNFA 120
           R  PYGITFPG PSGR SDGRVLTDY+  Y       P ++ Q     + R   NG+NFA
Sbjct: 61  RNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPR---NGINFA 120

Query: 121 FGGTGVFNTSVSFPNMTTQIDLFHNFIDET-----DIQSSIALVSVSGNDYSFYLAHNGS 180
           FGGTG+F+TS  FPNMTTQI+ F   I  +     DI SSIALVSVSGNDYSFY A NGS
Sbjct: 121 FGGTGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGS 180

Query: 181 LEGLKGFMISVVDQIIWNLKRILSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSNINSL 240
           +EG K F++SVV+QI  NLKRI  LGV+KVV+ GL PLGCLP  T +SSF++CN+ ++SL
Sbjct: 181 MEGFKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSL 240

Query: 241 VTFHNLLLNQSISKLNNNNETTTKNNINFFILDMYDSFMSIL--FGNPKQGG---EKPF- 300
           V FHN LL Q++ KLN   + +      FFILD+Y + +SI+   G+P +     E P  
Sbjct: 241 VEFHNALLKQAVEKLNKETKGSP-----FFILDIYGTLLSIIQNKGSPYKARPRFETPLK 300

Query: 301 -------DEFSCGSVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAFTTLQFILKNL 331
                   EFSCGSVD+KGNKK++LC+DPK A FWDS+HPTQ+GWF AF +L   LK L
Sbjct: 301 PCCFGVSSEFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLKKL 351

BLAST of PI0006227 vs. ExPASy TrEMBL
Match: A0A6J1I6N6 (GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111471216 PE=3 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 7.1e-91
Identity = 193/354 (54.52%), Postives = 240/354 (67.80%), Query Frame = 0

Query: 1   MDSQKLLF---SLFIILLLSDQGSGGHN-DLLREFQPKKLYVFGDSYVDTGNIGISNYSS 60
           M+S+K +F   SLF+  LL  +GSG H+ D      P KL+VFGDSYVDTGNI  +N SS
Sbjct: 1   MESRKTVFPLLSLFVFSLLLGRGSGRHHVDYRFNSWPTKLFVFGDSYVDTGNIKATNSSS 60

Query: 61  RKFPYGITFPGKPSGRFSDGRVLTDYVVSY-PISSLQRF---KRSKERKNINGVNFAFGG 120
           R  PYGITFPG PSGRFSDGRV+TD+  +Y  + S + F   ++   +    G+NFAFGG
Sbjct: 61  RNLPYGITFPGVPSGRFSDGRVMTDFFANYLELKSPETFIGWRKGGRKGPKYGMNFAFGG 120

Query: 121 TGVFNTSVSFPNMTTQIDLF-----HNFIDETDIQSSIALVSVSGNDYSFYLAHNGSLEG 180
           TGVF+TS  FPNMTTQID F     +N  D   + SS+ALVS SGNDYSFY   NGS+EG
Sbjct: 121 TGVFDTSFPFPNMTTQIDFFQGLVANNIFDPKHVGSSVALVSPSGNDYSFYQERNGSMEG 180

Query: 181 LKGFMISVVDQIIWNLKRILSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSNINSLVTF 240
           L+ F+ISVV+QI  NLKRI SLGVKK+VV GL P+GCLP  T +SSF  CNS  NS+   
Sbjct: 181 LQPFIISVVNQIAVNLKRIQSLGVKKIVVLGLGPVGCLPQPTANSSFKHCNSTTNSMAKV 240

Query: 241 HNLLLNQSISKLNNNNETTTKNNINFFILDMYDSFMSIL--FGNPKQGGE-----KP--- 300
           HNLLL Q++ KLN + + +      F I D+Y++ +SI+   G+PK         KP   
Sbjct: 241 HNLLLKQAVEKLNKDTKGSP-----FIIFDVYNAILSIIRNKGSPKARARFVTPLKPCCF 300

Query: 301 --FDEFSCGSVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAFTTLQFILKN 330
               EFSCGSVDEKGNK ++LC+ PKSA FWDS+HPTQQGWFA F++L   LK+
Sbjct: 301 GVSSEFSCGSVDEKGNKMYTLCKRPKSALFWDSVHPTQQGWFATFSSLASSLKH 349

BLAST of PI0006227 vs. ExPASy TrEMBL
Match: A0A0A0KIM6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G452040 PE=3 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 2.1e-90
Identity = 194/354 (54.80%), Postives = 234/354 (66.10%), Query Frame = 0

Query: 1   MDSQKLLFSLFIILLLS-DQGSGGHNDLLREFQPKKLYVFGDSYVDTGNIGISNYSSRKF 60
           M S  LLFS   +LL S   G G  + +   F P KL+VFGDSYVDTGNI ++  S+R F
Sbjct: 1   MASNNLLFSFLSLLLFSLHLGQGSSSPVHAHFYPLKLFVFGDSYVDTGNINVNTSSARNF 60

Query: 61  PYGITFPGKPSGRFSDGRVLTDYVVSY------PISSLQRFKRSKERKNINGVNFAFGGT 120
           PYGITFPG PSGRFSDGRVLTD++ +Y      PI      K   + +   G+NFAFGGT
Sbjct: 61  PYGITFPGFPSGRFSDGRVLTDFLANYVGLKRSPIPFTAWKKTGSKLRAKCGINFAFGGT 120

Query: 121 GVFNTSVSFPNMTTQIDLFHNFIDET-----DIQSSIALVSVSGNDYSFYLAHNGSLEGL 180
           GVF+T   FPNMTTQI+ F N I  +     DI SSIALVS SGNDYSFYLA NGS EG+
Sbjct: 121 GVFDTLYPFPNMTTQINFFQNLIANSIFTSYDIHSSIALVSPSGNDYSFYLATNGSPEGV 180

Query: 181 KGFMISVVDQIIWNLKRILSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSNINSLVTFH 240
           K F+ISVV+QI  NLKRI  LGVKK+VV GL P+GC P  T + SF +CN  +NSL  FH
Sbjct: 181 KPFVISVVNQISVNLKRIYKLGVKKIVVVGLGPVGCYPSPT-APSFKKCNETMNSLAVFH 240

Query: 241 NLLLNQSISKLNNNNETTTKNNINFFILDMYDSFMSIL--FGNPKQGGE-----KP---- 300
           N LL Q++ KL  N+ET    + NFFIL+MYD+ +SI+   GNPK G       KP    
Sbjct: 241 NTLLKQAVEKL--NSETKLGGSPNFFILNMYDTVLSIIKNKGNPKVGATFQTPLKPCCFG 300

Query: 301 -FDEFSCGSVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAFTTLQFILKNL 331
               FSCGSVDE GNK ++LC+ P  A FWD++HPTQ+GWFA F +L   LK +
Sbjct: 301 VSSNFSCGSVDEHGNKMYTLCKRPDLALFWDTVHPTQKGWFATFNSLISTLKRI 351

BLAST of PI0006227 vs. NCBI nr
Match: XP_008462556.1 (PREDICTED: GDSL esterase/lipase At5g03610-like [Cucumis melo])

HSP 1 Score: 581.6 bits (1498), Expect = 4.2e-162
Identity = 298/350 (85.14%), Postives = 309/350 (88.29%), Query Frame = 0

Query: 1   MDSQKLLFSLFIILLLSDQGSGGHNDLLREFQPKKLYVFGDSYVDTGNIGISNYSSRKFP 60
           MDSQKLLFSLF ILLLS+QGS GHNDL+REF+PKKLYVFGDSYVDTGNIGISNYSSRK+P
Sbjct: 1   MDSQKLLFSLFTILLLSEQGSAGHNDLIREFRPKKLYVFGDSYVDTGNIGISNYSSRKYP 60

Query: 61  YGITFPGKPSGRFSDGRVLTDYVVSY-----PISSLQRFKRSKER----KNINGVNFAFG 120
           YGITFPGKPSGRFSDGRVLTDYV  Y     PI     FK  K R    KNINGVNFA+G
Sbjct: 61  YGITFPGKPSGRFSDGRVLTDYVARYLGLKPPIPFRVLFKDLKRRQGRKKNINGVNFAYG 120

Query: 121 GTGVFNTSVSFPNMTTQIDLFHNFIDETDIQSSIALVSVSGNDYSFYLAHNGSLEGLKGF 180
           GTGVFNTSVSFPNMTTQIDLFH FIDETDIQSSIALVSVSGNDYSFYLA NGSL+G KGF
Sbjct: 121 GTGVFNTSVSFPNMTTQIDLFHKFIDETDIQSSIALVSVSGNDYSFYLARNGSLQGFKGF 180

Query: 181 MISVVDQIIWNLKRILSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSNINSLVTFHNLL 240
           MISVVDQIIWNLKRI SLGVKKV+VTGLAPLGCLPHFTNSSSFTQCNS+INSLVTFHNLL
Sbjct: 181 MISVVDQIIWNLKRIHSLGVKKVIVTGLAPLGCLPHFTNSSSFTQCNSDINSLVTFHNLL 240

Query: 241 LNQSISKL-NNNNETTTKNNINFFILDMYDSFMSILFGNPKQGGEKPF----------DE 300
           LNQSISKL NNNNETTTKNN NFFILDMY SFMSI+FGNPKQGG+KP           D 
Sbjct: 241 LNQSISKLNNNNNETTTKNNTNFFILDMYASFMSIIFGNPKQGGKKPLLLKPCCFGVSDA 300

Query: 301 FSCGSVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAFTTLQFILKNL 331
           FSCGSVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAF+ LQFILKNL
Sbjct: 301 FSCGSVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAFSALQFILKNL 350

BLAST of PI0006227 vs. NCBI nr
Match: XP_031742898.1 (GDSL esterase/lipase At5g03610 [Cucumis sativus])

HSP 1 Score: 570.5 bits (1469), Expect = 9.6e-159
Identity = 289/355 (81.41%), Postives = 307/355 (86.48%), Query Frame = 0

Query: 3   SQKLLFSLFIILLLSDQGSGGHND-LLREFQPKKLYVFGDSYVDTGNIGISNYSSRKFPY 62
           SQKLLFSLF + LLS+QGSGGHND LLREFQPKKLYVFGDSYVDTGNIGISN+SSRKFPY
Sbjct: 4   SQKLLFSLFTVFLLSEQGSGGHNDNLLREFQPKKLYVFGDSYVDTGNIGISNFSSRKFPY 63

Query: 63  GITFPGKPSGRFSDGRVLTDYV----------------VSYPISSLQRFKRSKERKNING 122
           GITFPGKPSGRFSDGRVLTDYV                + + +    + ++ ++ KNING
Sbjct: 64  GITFPGKPSGRFSDGRVLTDYVGKNMQLVSRYLGLKPPIPFRVFKDLKRRQGRKNKNING 123

Query: 123 VNFAFGGTGVFNTSVSFPNMTTQIDLFHNFIDETDIQSSIALVSVSGNDYSFYLAHNGSL 182
           VNFA+GGTGVFNTSVSFPNMTTQIDLFH FIDETDIQSSIALVSVSGNDYSFYLA NGSL
Sbjct: 124 VNFAYGGTGVFNTSVSFPNMTTQIDLFHKFIDETDIQSSIALVSVSGNDYSFYLARNGSL 183

Query: 183 EGLKGFMISVVDQIIWNLKRILSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSNINSLV 242
           +G KGFMISVVDQI+WNLKR+ SLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNS+INSLV
Sbjct: 184 QGFKGFMISVVDQIMWNLKRVHSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSDINSLV 243

Query: 243 TFHNLLLNQSISKLNNNNETTTKNNINFFILDMYDSFMSILFGNPKQGGEKPF------- 302
           TFHNLLLNQSISKLNNNNETTTKNN NFFILDMY SFMSI+FGNPKQGGEKP        
Sbjct: 244 TFHNLLLNQSISKLNNNNETTTKNNTNFFILDMYASFMSIIFGNPKQGGEKPLLLKPCCF 303

Query: 303 ---DEFSCGSVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAFTTLQFILKNL 331
              D FSCGSVDEKGNKKFSLCEDPKSAFFWDS HPTQQGWFAAF+TLQ ILKNL
Sbjct: 304 GVSDGFSCGSVDEKGNKKFSLCEDPKSAFFWDSFHPTQQGWFAAFSTLQSILKNL 358

BLAST of PI0006227 vs. NCBI nr
Match: KAE8647419.1 (hypothetical protein Csa_004424 [Cucumis sativus])

HSP 1 Score: 558.5 bits (1438), Expect = 3.8e-155
Identity = 283/336 (84.23%), Postives = 296/336 (88.10%), Query Frame = 0

Query: 15  LLSDQGSGGHND-LLREFQPKKLYVFGDSYVDTGNIGISNYSSRKFPYGITFPGKPSGRF 74
           L+S+QGSGGHND LLREFQPKKLYVFGDSYVDTGNIGISN+SSRKFPYGITFPGKPSGRF
Sbjct: 342 LVSEQGSGGHNDNLLREFQPKKLYVFGDSYVDTGNIGISNFSSRKFPYGITFPGKPSGRF 401

Query: 75  SDGRVLTDYVVSY-------PISSLQRFKR--SKERKNINGVNFAFGGTGVFNTSVSFPN 134
           SDGRVLTDYV  Y       P    +  KR   ++ KNINGVNFA+GGTGVFNTSVSFPN
Sbjct: 402 SDGRVLTDYVARYLGLKPPIPFRVFKDLKRRQGRKNKNINGVNFAYGGTGVFNTSVSFPN 461

Query: 135 MTTQIDLFHNFIDETDIQSSIALVSVSGNDYSFYLAHNGSLEGLKGFMISVVDQIIWNLK 194
           MTTQIDLFH FIDETDIQSSIALVSVSGNDYSFYLA NGSL+G KGFMISVVDQI+WNLK
Sbjct: 462 MTTQIDLFHKFIDETDIQSSIALVSVSGNDYSFYLARNGSLQGFKGFMISVVDQIMWNLK 521

Query: 195 RILSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSNINSLVTFHNLLLNQSISKLNNNNE 254
           R+ SLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNS+INSLVTFHNLLLNQSISKLNNNNE
Sbjct: 522 RVHSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSDINSLVTFHNLLLNQSISKLNNNNE 581

Query: 255 TTTKNNINFFILDMYDSFMSILFGNPKQGGEKPF----------DEFSCGSVDEKGNKKF 314
           TTTKNN NFFILDMY SFMSI+FGNPKQGGEKP           D FSCGSVDEKGNKKF
Sbjct: 582 TTTKNNTNFFILDMYASFMSIIFGNPKQGGEKPLLLKPCCFGVSDGFSCGSVDEKGNKKF 641

Query: 315 SLCEDPKSAFFWDSLHPTQQGWFAAFTTLQFILKNL 331
           SLCEDPKSAFFWDS HPTQQGWFAAF+TLQ ILKNL
Sbjct: 642 SLCEDPKSAFFWDSFHPTQQGWFAAFSTLQSILKNL 677

BLAST of PI0006227 vs. NCBI nr
Match: XP_038883507.1 (GDSL esterase/lipase At5g03610-like [Benincasa hispida])

HSP 1 Score: 357.8 bits (917), Expect = 9.8e-95
Identity = 204/358 (56.98%), Postives = 243/358 (67.88%), Query Frame = 0

Query: 1   MDSQKLLF---SLFIILLLSDQGSGGHNDLLREFQPKKLYVFGDSYVDTGNIGISNYSSR 60
           M S+KLLF   SL I  LLS QGS GH  +     P KL VFGDSYVDTGNI ++   +R
Sbjct: 2   MASKKLLFTFLSLLIFSLLSGQGSAGHFHI----HPTKLIVFGDSYVDTGNINVNTSGAR 61

Query: 61  KFPYGITFPGKPSGRFSDGRVLTDYVVSY--------PISSLQRFKRSKERKNINGVNFA 120
            FPYGITFPG PSGRFSDGRVLTD+   Y        P  + Q+    + +    G+NFA
Sbjct: 62  NFPYGITFPGVPSGRFSDGRVLTDFFAKYLGLKKSPTPFIAWQKIGNKRPK---FGINFA 121

Query: 121 FGGTGVFNTSVSFPNMTTQIDLFHNFIDET-----DIQSSIALVSVSGNDYSFYLAHNGS 180
           FGGTGVF+TS  FPNMTTQI+LF N I  +     DI SSIALVS SGNDYSFYLA NGS
Sbjct: 122 FGGTGVFDTSFPFPNMTTQINLFQNLIANSIFTSYDIHSSIALVSPSGNDYSFYLATNGS 181

Query: 181 LEGLKGFMISVVDQIIWNLKRILSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSNINSL 240
            +G K F+ISVV+QI  NLKRI  LGVKK+VV GL P+GC P  T + SF +CN+ +NS 
Sbjct: 182 PQGFKPFVISVVNQIAVNLKRIYRLGVKKIVVVGLGPVGCYPQLT-APSFNKCNATMNSF 241

Query: 241 VTFHNLLLNQSISKLNNNNETTTKNNINFFILDMYDSFMSIL--FGNPKQGGE-----KP 300
           V FHN+LL +++ KLNN     TK + NFFILDMYD+ +SI+   GNPK G       KP
Sbjct: 242 VEFHNILLKKAVEKLNNE----TKGSPNFFILDMYDTVLSIIQNKGNPKVGVSFKTPLKP 301

Query: 301 -----FDEFSCGSVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAFTTLQFILKNL 331
                  EFSCGSVDE GNK ++LC+ P  A FWDSLHPTQ+GWFAAF++L+  LK+L
Sbjct: 302 CCFGVSSEFSCGSVDEHGNKMYTLCKHPNLALFWDSLHPTQKGWFAAFSSLRSNLKHL 347

BLAST of PI0006227 vs. NCBI nr
Match: XP_022133081.1 (GDSL esterase/lipase At5g03610-like [Momordica charantia])

HSP 1 Score: 350.9 bits (899), Expect = 1.2e-92
Identity = 195/359 (54.32%), Postives = 242/359 (67.41%), Query Frame = 0

Query: 1   MDSQKLLFSLFIIL----LLSDQGSGGHNDLLREFQPKKLYVFGDSYVDTGNIGISNYSS 60
           MDS+KLLFSL   L    LL  QGS  ++      +P +L+VFGDSYVDTGNI ++  S+
Sbjct: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRHFHLRPTRLFVFGDSYVDTGNINVNTSSA 60

Query: 61  RKFPYGITFPGKPSGRFSDGRVLTDYVVSY-------PISSLQRFKRSKERKNINGVNFA 120
           R  PYGITFPG PSGR SDGRVLTDY+  Y       P ++ Q     + R   NG+NFA
Sbjct: 61  RNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPR---NGINFA 120

Query: 121 FGGTGVFNTSVSFPNMTTQIDLFHNFIDET-----DIQSSIALVSVSGNDYSFYLAHNGS 180
           FGGTG+F+TS  FPNMTTQI+ F   I  +     DI SSIALVSVSGNDYSFY A NGS
Sbjct: 121 FGGTGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGS 180

Query: 181 LEGLKGFMISVVDQIIWNLKRILSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSNINSL 240
           +EG K F++SVV+QI  NLKRI  LGV+KVV+ GL PLGCLP  T +SSF++CN+ ++SL
Sbjct: 181 MEGFKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSL 240

Query: 241 VTFHNLLLNQSISKLNNNNETTTKNNINFFILDMYDSFMSIL--FGNPKQGG---EKPF- 300
           V FHN LL Q++ KLN   + +      FFILD+Y + +SI+   G+P +     E P  
Sbjct: 241 VEFHNALLKQAVEKLNKETKGSP-----FFILDIYGTLLSIIQNKGSPYKARPRFETPLK 300

Query: 301 -------DEFSCGSVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAFTTLQFILKNL 331
                   EFSCGSVD+KGNKK++LC+DPK A FWDS+HPTQ+GWF AF +L   LK L
Sbjct: 301 PCCFGVSSEFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLKKL 351

BLAST of PI0006227 vs. TAIR 10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 299.3 bits (765), Expect = 3.8e-81
Identity = 171/355 (48.17%), Postives = 221/355 (62.25%), Query Frame = 0

Query: 1   MDSQ-KLLFSLFIIL---LLSDQGSG----GHNDLLREFQPKKLYVFGDSYVDTGNIGIS 60
           MDS  KL F LFI L   LL  + +G      N  L  F+P KL+VFGDSY DTGNI  +
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLFGEINGVEGSNQNHHLYPFRPTKLFVFGDSYADTGNIKKA 60

Query: 61  NYSSRKFPYGITFPGKPSGRFSDGRVLTDYVVSY-----PISSLQRFKRSKERKNINGVN 120
             SS KFPYGITFPGKP+GRFSDGRV TD++  +     PI    +    K+R    G+N
Sbjct: 61  FSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRLQY-GMN 120

Query: 121 FAFGGTGVFNTSVSFPNMTTQIDLFHNFIDETDI------QSSIALVSVSGNDYSFYLAH 180
           FA+GGTGVFNT    PNMTTQID+F N +   DI       SS+ALVSV+GNDYS ++A 
Sbjct: 121 FAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVAGNDYSNFIAL 180

Query: 181 NGSLEGLKGFMISVVDQIIWNLKRILSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSNI 240
           N        F+  VVDQ   NL+RI +LGVKK+ V  L PLGCLP FT  +SF +CN   
Sbjct: 181 NRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNETQ 240

Query: 241 NSLVTFHNLLLNQSISKLNNNNETTTKNNINFFILDMYDSFMSILFGNPKQGGEKPFD-- 300
           N+LV  HN LL Q ++KLNN  + +T     F ILD+Y++F+++        G   F+  
Sbjct: 241 NALVNLHNNLLQQVVAKLNNETKQST-----FIILDLYNAFLTVFKNKGSNPGSTRFESP 300

Query: 301 ----------EFSCGSVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAFTTLQ 325
                     E++CGSVDEKG KK+ +C++PK+AFFWD LHPT++GW + ++ L+
Sbjct: 301 LKPCCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWRSVYSVLR 349

BLAST of PI0006227 vs. TAIR 10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 273.5 bits (698), Expect = 2.3e-73
Identity = 159/359 (44.29%), Postives = 213/359 (59.33%), Query Frame = 0

Query: 1   MDSQKLLFSLFII----LLLSDQGSGGHNDLLREFQPKKLYVFGDSYVDTGNIGISNYSS 60
           M+  KLL SLF+       L  +     +  L+  +P +L+VFGDSY DTGNI  S   S
Sbjct: 1   MELPKLLISLFLFSFSSFFLGAESDYPQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSDS 60

Query: 61  RKFPYGITFPGKPSGRFSDGRVLTDYVVSY-----PISSLQRFKRSKERKNINGVNFAFG 120
            K PYGITFP KPSGRFSDGRV TD++  Y     PI    +    KER  + G+N+A+G
Sbjct: 61  WKIPYGITFPQKPSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERL-LYGMNYAYG 120

Query: 121 GTGVFNTSVS-FPNMTTQIDLFH------NFIDETDIQSSIALVSVSGNDYSFYLAHNGS 180
           GTGVF T  +  PNMTTQID F       N    +D+ SS+ALVSV+GNDY+ +LA    
Sbjct: 121 GTGVFKTKDNPLPNMTTQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRP 180

Query: 181 LEGLKGFMISVVDQIIWNLKRILSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSNINSL 240
           L  L  FM  VVDQI  N  RI  LGV K+V+  + PLGCLP  T  +SF +CN+  N+ 
Sbjct: 181 LTELPAFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNAS 240

Query: 241 VTFHNLLLNQSISKLNNNNETTTKNNINFFILDMYDSFMSIL-----------FGNPKQ- 300
              HN LL+++I++LNN  + +T     F +LD Y++F+++            FGNP + 
Sbjct: 241 TNLHNYLLHKAIARLNNETKPST-----FVVLDHYNAFLTVFKNKGPEPGVSRFGNPLKP 300

Query: 301 ---GGEKPFDEFSCGSVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAFTTLQFILK 329
              G    +D   C +VDEKG KK+ +CEDPK+AFFWD  HP+++GW + ++ L   LK
Sbjct: 301 CCVGVNSSYD---CSNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVYSVLHKHLK 350

BLAST of PI0006227 vs. TAIR 10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 214.5 bits (545), Expect = 1.2e-55
Identity = 121/314 (38.54%), Postives = 187/314 (59.55%), Query Frame = 0

Query: 23  GHNDLLREFQPKKLYVFGDSYVDTGNIGISNYSSRKFPYGITFPGKPSGRFSDGRVLTDY 82
           G N LLR  +  KL+VFGDSY DTGN    +  +   PYGITFPGKPSGR+ DG + TD+
Sbjct: 2   GSNQLLRPRKVPKLFVFGDSYADTGNTK-RDTEAWAIPYGITFPGKPSGRYCDGLIATDF 61

Query: 83  ---VVSYPISSLQRFKRSKERKNINGVNFAFGGTGVFNTSVS--FPNMTTQIDLFHNFID 142
              V+      L R    +++    G+NFAFGG+ + ++S +  FPN+T Q++   + + 
Sbjct: 62  LEKVLGAESPYLYR-THGRDKGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVL 121

Query: 143 ETDI-----QSSIALVSVSGNDYSFYLAHNGSLEGLKGFMISVVDQIIWNLKRILSLGVK 202
              +      S ++L+S +G DY +Y+  N    GLK  +  VVD +  N+  +  L  K
Sbjct: 122 AGRVYGDITPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFK 181

Query: 203 KVVVTGLAPLGCLPHFTNSSSFTQCNSNINSLVTFHNLLLNQSISKLNNNNETTTKNNIN 262
           K+ VT L P+GCLP +T++SSF  CN + ++LV  HN LL + ++KLN  +    K   +
Sbjct: 182 KIAVTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQ-H 241

Query: 263 FFILDMYDSFMSILFGNPKQGGEKPFD---EFSCGSVDEKGNKKFSLCEDPKSAFFWDSL 322
           FFI+D++++FM+++     +  + P     E  CG   + G K ++LC+DPKS FFWD++
Sbjct: 242 FFIIDIHNAFMTVMKNKGSKRFKNPMKSCCEGYCGRSSD-GGKLYTLCDDPKSFFFWDAV 301

Query: 323 HPTQQGWFAAFTTL 324
           HPTQ+GW + ++ L
Sbjct: 302 HPTQEGWRSIYSVL 311

BLAST of PI0006227 vs. TAIR 10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 201.8 bits (512), Expect = 8.4e-52
Identity = 130/316 (41.14%), Postives = 171/316 (54.11%), Query Frame = 0

Query: 31  FQPKKLYVFGDSYVDTGNIGISNYSSRKFPYGITFPGKPSGRFSDGRVLTDYVVSY---- 90
           F+P KL+VFGDSY DTGN       S +FP GITFPG P+GRFSDGRV TDY+  Y    
Sbjct: 43  FKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVR 102

Query: 91  -PISSLQRFKRSKERKNI-NGVNFAFGGTGVFNTSVSF-PNMTTQID-----LFHNFIDE 150
            PI + +  K  + R  +  G+NFA+GG G F T     P  + QID     L  N    
Sbjct: 103 TPI-TYKWGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRNVYSP 162

Query: 151 TDIQSSIALVSVSGNDYSFYLAHNGSLEGLKGFMISVVDQIIWNLKRILSLGVKKVVVTG 210
            D+ SS+A  S+ GNDY  Y   NGS EG       VV QI+ ++KRI  LGV+KV+V  
Sbjct: 163 ADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVAL 222

Query: 211 LAPLGCLPHFTNSSSFTQCNSNINSLVTFHNLLLNQSISKLNNNNETTTKNNINFFILDM 270
             P  CLP          C++N  S    HN LL + + KL  N++    N+ +F  LD+
Sbjct: 223 SPPQKCLPKLVTPKG---CDTNDTS-TYLHNSLLRKGLIKL--NDKEINNNDKSFMTLDL 282

Query: 271 YDSFMSILFGNPKQGGEKPF-DEFS----------CGSVDEKGNKKFSLCEDPKSAFFWD 324
           Y++F++I F N    G   F D F           CG     G K ++LC+DPKS FFWD
Sbjct: 283 YNAFVTI-FKNKGVSGVSTFPDPFKACCATKRGTFCGDRSLSGKKLYTLCDDPKSFFFWD 342

BLAST of PI0006227 vs. TAIR 10
Match: AT5G03590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 131.0 bits (328), Expect = 1.8e-30
Identity = 90/279 (32.26%), Postives = 135/279 (48.39%), Query Frame = 0

Query: 56  SRKFPYGITFPGKPSGRFSDGRVLTDYVVSYPISSLQRFKRSKERKNINGVNFAFGGTGV 115
           S K PYGITFPGKPSGR+SDG   TD++                            G+ V
Sbjct: 7   SWKLPYGITFPGKPSGRYSDGLTATDFL----------------------------GSEV 66

Query: 116 FNTSVS-FPNMTTQIDLFHN-------FIDETDIQSSIALVSVSGNDYSFYLAHNGSLEG 175
           F++ V   PN++TQ+    N       +  + D+ SS AL+S SG DY  ++  N ++  
Sbjct: 67  FDSPVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGFIDQNPNMAA 126

Query: 176 LKGFMISVVDQIIWNLKRILSLGVKKVVVTGLAPLGCLPHFTNSSSFTQCNSNINSLVTF 235
              F+  +V+ I                                     CN + + LV  
Sbjct: 127 YPAFVEFIVEDI------------------------------------HCNESYSDLVRL 186

Query: 236 HNLLLNQSISKLNNNNETTTKNNINFFILDMYDSFMSIL--FGNPK-QGGEKPFDEFSCG 295
           HN  L ++++KLN  ++  TK +  F I+D++ +FM+IL   GN + +   KP  E  C 
Sbjct: 187 HNESLKKAVAKLNKEDKFRTKGD-RFVIVDLHKAFMTILEKKGNKRFKSPLKPCCEGDCA 220

Query: 296 SVDEKGNKKFSLCEDPKSAFFWDSLHPTQQGWFAAFTTL 324
            +D KG KK++LC DPKSAFFWD ++PTQ+GW + ++ L
Sbjct: 247 RMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSIYSLL 220