PI0006193 (gene) Melon (PI 482460) v1

Overview
NamePI0006193
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionamidase 1-like
Locationchr02: 908095 .. 912533 (-)
RNA-Seq ExpressionPI0006193
SyntenyPI0006193
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGATAAGCCGTTGTACTATTTTGTTTCCCTTCTTCATCATCATCTTCTTCATCATCTTCTTTGCTTGCCCTAGCTTATCTTCCGCTCTGCAATTTTCCAGCCATTATCAAGGAGGTTGTGGCGGACTCTGATTCCAATTAGATTCAAAGAAGAAGGGGAGGAGCAAAAAACCTCACGTTTGGCAATGGCCGTGCAGCATTATGGAGCTTTCATGGAGAAATTCCTACTCCAACCGAGCTCCCCCAACGATCAACTTCCCTTGACTGGCCTCACTTTCGCTGTTAAAGACATGTGAGATACTTTTTTTATTTACTGTTTTTATTTGGTTCTGGAAATTCATGAAGGATTTGGTGATAACAGGTTGTGAGTTGTGTAACATTTTTAGTATTCTGGATATTGTCAAAGGATTTTAATTTTTATTGTAGGTGGAATAGATGTTTCTCTCCGATATTATTTCTAGGATCAACATATATGTTCATGAGATGAATGGATTGTTCTTTGGGGAAAATTCTATTTGTTGTACTAATGAATAGATTTATTAATTAATCAATAGTTTAGATATTAGTATTTGCATTCTTACAAGCCATCAACAGTTGGTTTTCTATTGTAATGGTTCAGTAATGTGTGCAAGATGAAGCTTATTTCATTGTAGTGATTGTTGGTTCTTTCAGTTCTTTGTTTACTTTATGCATGGTCCTTCAGATATAAGTGTGTCAGTTGTTCTTCATGAAATTGGGGTATTGATGTCAAAGTTCTCACTGGGATTTGAACCAAACAGATTTGATATGGATGGCCATGTAACTGGTTTTGGAAATCCTGAATGGTTAAGGACTCACCCACCTGCCAATCATACTGCCCCAACTGTGTCGACCATCCTAAGAGGAGGAGCCACATGCATTGGCAAGACTATTATGGATGAAATGGCCTACAGGTACCAAGAAAATCCAACTCTTATTCCCTTTTTCCTCTTTAGCCTTGTTTTCTTTTTAACAATTGATAAACGACTAAAGAAGGCATAAGCTTTACAAATTGGGATACAGAGGATACAAATTACTATTTTGCGCTGCACACTATTCTATAATAGGTCTTAAAACTATGTCTATTCCATTCTTGTCATTCTATTTCAAACAGACAACTCTGCATTGCAATGATCAAAATATGAACTAAACCAATTAACTTATGTCAGGTTTCTAATTTCTAGTTGAAAAACTTTCACCAAATGATTTTTCCTGACATCGTAGGCCTTTGAATCTTTTCAATGGTGGAATCACTAGTTTGATAATCGTATTTTCGCAGTATAAATGGAGAAAACTTTCACTACGGCACACCCCAAAACCCATGTGCATCAGATCGTGTACCTGGAGGATCTTCCAGTGGCTCTGCTGTTGCTGTAGGTGCAAATCTTGTGGATTTCTCCTTAGGCACGTTCTTAGTTCTTTGACCTTGAATAATTTGTGGTCTAATGACTTCTGCCTGGAAAATGTTGTAAAAGATCATATTTAGTTTTGTAGGAACTGATACTGGAGGTAGTGTAAGAGTGCCGGCATCCTATTGTGGAATACTTGGATTTCGGCCTTCACATGGTGCTGTCTCTACCTCTGGAGTAGTACCCATGGCACAGAGCTTTGATACAGTAGGTAATGAATAGTGTCTCATTGTCACTTTGTTTACATCTTTTGTAATCACAGAACTGTTGGGACGACTTTGTACTCGTGATTTTGCTTATATGAGTGATACAGTATTGATTGGTACTAAAGGCATTGGTTGCTTATAAATAGAATAGAGCCCATATCTCAACAAAATGCCATCTTTCTGAACATGTTATCTCCTCTTCCTTCAAGGCTCTATCATCAATTTTGCATTCATGATGTAACGCCATCTATCCTGTCATATTAAAAAAGCTTACCCTTTGACACCATCTTTGCATTTTAGGATGGTTTGCCAGGGATCCTGTTGTATTAAAAAAAGTAGGTCATCTGCTGCTGCAACAGCCAGAGGTTGAACATCAAAAGCCTACACAGGTGCTTATAGCAGAAGATTGTTTCAAGCTCTCAAGCATTCCTAGTGAACGATTGACACAAGCTTTTGTTAATTCAGTAAAGAAGTTATTTGGTGGTGAGTCAACATTCCATACCAGTTGTCTTCACAGGTTGTGTCTTGATGAATGTATATTTTACAATGGTTCATACATGAATTGGAATTTGATTTCTCTTGCATTTAAGTTTCTCACTTGTTGAGTTTAACAACATTTGGGGGTAGAAATTTGAATCTTTGACCTTTTGGTCGATGATATATGCTTTAACCATTTGAGTTATGCTTTGGTTGACATTCATTTTAGGCATCTCATTTGCTCGAGAGGGAATTTATTAGAGGATCTAGCTTCATACTTTGAGATGTCTGCCATTTCTTAGACTATAGCCTTTTTGTCACATTCAACAAAAGATCGATTCAGAGCCAAAGCTTTGCAAACGGTATATGGGATCATTGATAGTAATATGGAAGAAAATGATAAACATAGTATTTGAGTTCTTTTGCTCTTCCTGATATGATTTTTCAAGCGAGTTGGAAACTTTTTTATATGAGCCTTCAACTGCAGGCCATCTTATAAAACAAGTAAGCCTTGGGAATTATGTTGAGGAAAAAGTTCCAAGTTTGAAGCATTTCATGATTGAAGGAAATGCAGGCAATGAGCATAGCATACCGTCCTTGGCAGCCCTTGCAAGATCGCTGCAATTGCTTCAAAGGTTCTGATCTCACCATGAATCGTAACTCGAATTCTGTTGAGCTGGATTGATGATGATATATTTTTGTGTTGAATGAATGATTGATCTTCGTCCTCCTCTCTTTTTACTTCCCCTTTGTAGGTATGAATTCAAAATCAATCACGAAGAATGGGTTAGAACTTACAACCCTCATTTGGGCCCAGGAATATCAGAACGAGTATCAGAAGCCATGAGGGCAACAGATGAGAACATTGATCTGTGTCATTCCATTAAAATCGAACTGCGCAAAGCTCTTGCTGCACTTCTTGAAGTACTCTCTCTATCTTTCTTGCATGCTTAATGTACTAAAACTTCAAAGTAAAATAGTTTGCAACTTAACCATCATGAGTTGGCCTAGCGCTAAATAGGGTAGTAATAGGTCATCGGTTGCATCCATGTTGGCCACCTATTTAGGATTTTAATATCATAAAAATTTTCTTGACACTCAAATGTTGTAAGATTAGGGAGGTTGCCAATTGAGATTAGTTGAGGTGCACGTAAGCTGGTTCGAACACTCACAAATATAAATTGCAAACTTAAAATGTATGCATTTTTCTTTTTGTTAGAGAAAAATCTTATTGACCGAAAGAGCAATTTGTGATTGAAATGGATTACTTTTATTTAGGAATGTCAATCGTGGCATATTTATAATTATTTGAGAAGTTAATTTTAGTTGACGGGTATAAATAGTGGGAACTAGGATAGGCAAAATTTTGGAAATTAGTTTTGAGGGAGAGCTCCTCAAAAGACTTGAGTCATAGGTTTGAATCTCCACTCTTTTGTTTGGAAAAGAATATATATGTAAAATGTTTTGGATGACAAAGTATCGTATAACAATCATATGAAATTCTGCGGTGAAATTCGTTTTAGAATTAAGTGACAAATCAGACTTTGTTTGTTGTTGAATAGTCTTCTGTTGTTTCCATCTAAGGAGAGGCCATTTACTCTCTGGAAAAAGCAGTTTGTGAATCATACTTTTAAACAAATAATCTTGAGGTGCTATTTTGGATGATTTCATTTCAGGATTTTGGGGTCCTTGCAATTCCTACCGTCCCTGGTCCCGCTCCTAAACTAAACACAGACGTCTCAAAGCTGCACGACTTTCGTGCGAAGGCTTTCAGCTTGCTCTCCATTGCTGGGGTCTCTGGATTCTGCCAGGTTCTCTCTCTCTCTCTCTCTCTCAAAAGTTAAAAGCAACTATTTTGATGGTTTTTGTTTTCTCTGACTAAGTAATGATCTGCAGGTTAGCATACCTCTTGGGTTGTACAATGGTCTTCCTGTATCAATATCTTTGGTAGCAAATCATGGTTCAGATGGGTTTTTGCTTAATGTTGTTCATAGTCTTTACAACACATTAGAAGAAGTTAAGGCAAGCTTCTAAGAAAGACAAAAGGAAGCTAAAGGAAACTCCCTTGATATTTGAAAATTGATGTCTTATTTTTCAGATTGGAAGAATGAGAACATGTGCAAGGCAGGACTTATCCTATATATTGATAAGTTTTTCTTTTTGTTGCAATGAATAAATTTAAGTTCATTGGATGATGAAGATATTTTTATTTCATCATCTCATGTATTCTTTGTTTAGCTCTTGATAATGAATCTTTATTTCCGTGGGCTTCTTGACAATCCCACATTAAAAA

mRNA sequence

AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGATAAGCCGTTGTACTATTTTGTTTCCCTTCTTCATCATCATCTTCTTCATCATCTTCTTTGCTTGCCCTAGCTTATCTTCCGCTCTGCAATTTTCCAGCCATTATCAAGGAGGTTGTGGCGGACTCTGATTCCAATTAGATTCAAAGAAGAAGGGGAGGAGCAAAAAACCTCACGTTTGGCAATGGCCGTGCAGCATTATGGAGCTTTCATGGAGAAATTCCTACTCCAACCGAGCTCCCCCAACGATCAACTTCCCTTGACTGGCCTCACTTTCGCTGTTAAAGACATATTTGATATGGATGGCCATGTAACTGGTTTTGGAAATCCTGAATGGTTAAGGACTCACCCACCTGCCAATCATACTGCCCCAACTGTGTCGACCATCCTAAGAGGAGGAGCCACATGCATTGGCAAGACTATTATGGATGAAATGGCCTACAGTATAAATGGAGAAAACTTTCACTACGGCACACCCCAAAACCCATGTGCATCAGATCGTGTACCTGGAGGATCTTCCAGTGGCTCTGCTGTTGCTGTAGGTGCAAATCTTGTGGATTTCTCCTTAGGAACTGATACTGGAGGTAGTGTAAGAGTGCCGGCATCCTATTGTGGAATACTTGGATTTCGGCCTTCACATGGTGCTGTCTCTACCTCTGGAGTAGTACCCATGGCACAGAGCTTTGATACAGTAGGATGGTTTGCCAGGGATCCTGTTGTATTAAAAAAAGTAGGTCATCTGCTGCTGCAACAGCCAGAGGTTGAACATCAAAAGCCTACACAGGTGCTTATAGCAGAAGATTGTTTCAAGCTCTCAAGCATTCCTAGTGAACGATTGACACAAGCTTTTGTTAATTCAGTAAAGAAGTTATTTGGTGGCCATCTTATAAAACAAGTAAGCCTTGGGAATTATGTTGAGGAAAAAGTTCCAAGTTTGAAGCATTTCATGATTGAAGGAAATGCAGGCAATGAGCATAGCATACCGTCCTTGGCAGCCCTTGCAAGATCGCTGCAATTGCTTCAAAGGTATGAATTCAAAATCAATCACGAAGAATGGGTTAGAACTTACAACCCTCATTTGGGCCCAGGAATATCAGAACGAGTATCAGAAGCCATGAGGGCAACAGATGAGAACATTGATCTGTGTCATTCCATTAAAATCGAACTGCGCAAAGCTCTTGCTGCACTTCTTGAAGATTTTGGGGTCCTTGCAATTCCTACCGTCCCTGGTCCCGCTCCTAAACTAAACACAGACGTCTCAAAGCTGCACGACTTTCGTGCGAAGGCTTTCAGCTTGCTCTCCATTGCTGGGGTCTCTGGATTCTGCCAGGTTAGCATACCTCTTGGGTTGTACAATGGTCTTCCTGTATCAATATCTTTGGTAGCAAATCATGGTTCAGATGGGTTTTTGCTTAATGTTGTTCATAGTCTTTACAACACATTAGAAGAAGTTAAGGCAAGCTTCTAAGAAAGACAAAAGGAAGCTAAAGGAAACTCCCTTGATATTTGAAAATTGATGTCTTATTTTTCAGATTGGAAGAATGAGAACATGTGCAAGGCAGGACTTATCCTATATATTGATAAGTTTTTCTTTTTGTTGCAATGAATAAATTTAAGTTCATTGGATGATGAAGATATTTTTATTTCATCATCTCATGTATTCTTTGTTTAGCTCTTGATAATGAATCTTTATTTCCGTGGGCTTCTTGACAATCCCACATTAAAAA

Coding sequence (CDS)

ATGGCCGTGCAGCATTATGGAGCTTTCATGGAGAAATTCCTACTCCAACCGAGCTCCCCCAACGATCAACTTCCCTTGACTGGCCTCACTTTCGCTGTTAAAGACATATTTGATATGGATGGCCATGTAACTGGTTTTGGAAATCCTGAATGGTTAAGGACTCACCCACCTGCCAATCATACTGCCCCAACTGTGTCGACCATCCTAAGAGGAGGAGCCACATGCATTGGCAAGACTATTATGGATGAAATGGCCTACAGTATAAATGGAGAAAACTTTCACTACGGCACACCCCAAAACCCATGTGCATCAGATCGTGTACCTGGAGGATCTTCCAGTGGCTCTGCTGTTGCTGTAGGTGCAAATCTTGTGGATTTCTCCTTAGGAACTGATACTGGAGGTAGTGTAAGAGTGCCGGCATCCTATTGTGGAATACTTGGATTTCGGCCTTCACATGGTGCTGTCTCTACCTCTGGAGTAGTACCCATGGCACAGAGCTTTGATACAGTAGGATGGTTTGCCAGGGATCCTGTTGTATTAAAAAAAGTAGGTCATCTGCTGCTGCAACAGCCAGAGGTTGAACATCAAAAGCCTACACAGGTGCTTATAGCAGAAGATTGTTTCAAGCTCTCAAGCATTCCTAGTGAACGATTGACACAAGCTTTTGTTAATTCAGTAAAGAAGTTATTTGGTGGCCATCTTATAAAACAAGTAAGCCTTGGGAATTATGTTGAGGAAAAAGTTCCAAGTTTGAAGCATTTCATGATTGAAGGAAATGCAGGCAATGAGCATAGCATACCGTCCTTGGCAGCCCTTGCAAGATCGCTGCAATTGCTTCAAAGGTATGAATTCAAAATCAATCACGAAGAATGGGTTAGAACTTACAACCCTCATTTGGGCCCAGGAATATCAGAACGAGTATCAGAAGCCATGAGGGCAACAGATGAGAACATTGATCTGTGTCATTCCATTAAAATCGAACTGCGCAAAGCTCTTGCTGCACTTCTTGAAGATTTTGGGGTCCTTGCAATTCCTACCGTCCCTGGTCCCGCTCCTAAACTAAACACAGACGTCTCAAAGCTGCACGACTTTCGTGCGAAGGCTTTCAGCTTGCTCTCCATTGCTGGGGTCTCTGGATTCTGCCAGGTTAGCATACCTCTTGGGTTGTACAATGGTCTTCCTGTATCAATATCTTTGGTAGCAAATCATGGTTCAGATGGGTTTTTGCTTAATGTTGTTCATAGTCTTTACAACACATTAGAAGAAGTTAAGGCAAGCTTCTAA

Protein sequence

MAVQHYGAFMEKFLLQPSSPNDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHTAPTVSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVGANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVLKKVGHLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVSLGNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGPGISERVSEAMRATDENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNTDVSKLHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTLEEVKASF
Homology
BLAST of PI0006193 vs. ExPASy Swiss-Prot
Match: Q9FR37 (Amidase 1 OS=Arabidopsis thaliana OX=3702 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 1.8e-152
Identity = 270/415 (65.06%), Postives = 325/415 (78.31%), Query Frame = 0

Query: 6   YGAFMEKFLLQPSSPNDQLP-LTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHTAPT 65
           +GAF+EK  + P+S +   P L GLTFA+KDIFD++G VTGFGNP+WLRTH  A  TAP 
Sbjct: 7   FGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAPV 66

Query: 66  VSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVGANLV 125
           VS++L  GAT +G TIMDEMAYSINGEN HYGTP+NP A DRVPGGSSSGSAVAV A LV
Sbjct: 67  VSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARLV 126

Query: 126 DFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVLKKVG 185
           DFS+GTDTGGSVRVPASYCGI GFRPSHGAVST G+ PMAQSFDTVGWFARD   LK+VG
Sbjct: 127 DFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARDTATLKRVG 186

Query: 186 HLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGH-LIKQVSLGNY 245
            +LLQQ  +   +P+Q++IA+DCFKL S+P + L Q  V SV+K FGG+ ++K+V+LG Y
Sbjct: 187 CVLLQQHHLNPIEPSQLIIADDCFKLCSVPHDLLVQPLVGSVEKSFGGNTVVKKVNLGEY 246

Query: 246 VEEKVPSLKHFMIEGN--AGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP 305
           + + VPSLKHFM   +     E  IPSL AL+ S++LLQR+EFKINH  W+ +  P  GP
Sbjct: 247 IGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWISSVKPEFGP 306

Query: 306 GISERVSEAMRATDENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNTDVSKL 365
           GISER+ EA+R +DE ID C S+K EL  AL+ LL + GVL IPTVPGP P L  +V+ L
Sbjct: 307 GISERIEEAIRTSDEKIDHCRSVKSELITALSTLLGEKGVLVIPTVPGPPPHLQANVAAL 366

Query: 366 HDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSL 417
             FR++AFSLLSIAGVSGFCQVSIPLGL+  LPVS+SLVA +GSDGFLL++V SL
Sbjct: 367 ESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSLVDSL 421

BLAST of PI0006193 vs. ExPASy Swiss-Prot
Match: Q7XTK3 (Amidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 6.7e-139
Identity = 247/416 (59.38%), Postives = 307/416 (73.80%), Query Frame = 0

Query: 6   YGAFMEKFLLQPSSPNDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHTAPTV 65
           YGAFME+F+L P  P+ QLPL GLTFA+KDIFD+ G VTGFGNP+W RTH PA  T+P V
Sbjct: 11  YGAFMERFVL-PPPPSQQLPLHGLTFAIKDIFDIAGRVTGFGNPDWARTHAPAAATSPVV 70

Query: 66  STILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVGANLVD 125
              L  GAT +G TIMDEMAYSINGEN HYGTP NPCA  RVPGGSSSGSAVAV ANLVD
Sbjct: 71  LAALAAGATSLGTTIMDEMAYSINGENTHYGTPTNPCAPGRVPGGSSSGSAVAVAANLVD 130

Query: 126 FSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVLKKVGH 185
           FSLGTDTGGSVRVPA+YCGI G RPSHG VS   V+PMAQ FDTVGWF+RD   L +V  
Sbjct: 131 FSLGTDTGGSVRVPAAYCGIFGLRPSHGLVSAENVIPMAQMFDTVGWFSRDLSTLSRVTK 190

Query: 186 LLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVSLGNYVE 245
           +LL  P+   ++PTQV I  DCF++     +R  Q    SV K F   ++   +LG+++ 
Sbjct: 191 VLLPLPDDIVKQPTQVTIPMDCFQILGSLDDRTYQIINASVAKRFDSQILDNRNLGDFIS 250

Query: 246 EKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGPGISE 305
           + VPS+  F+ + +     S+P+L+ ++  ++ LQR +FK NH EWV T  P+LGPG+ E
Sbjct: 251 DNVPSIGKFITDFSESELPSVPALSVISHVMRGLQRSQFKANHAEWVNTVKPNLGPGLRE 310

Query: 306 RVSEAMRATD-ENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNTDVSKLHDF 365
           R+ EA+ + D E+++   +I+ E + ALAALL+D G+LAIPTVPGP PK+  + + L +F
Sbjct: 311 RILEAIASGDNESLEDFQAIRAEFKSALAALLKDHGILAIPTVPGPPPKVGMEAAPLENF 370

Query: 366 RAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTL 421
           RA+AFSLLSIAG+SGFCQVSIPLG+ NGLPVS+SLVA HG+D FLLNVV  LY TL
Sbjct: 371 RARAFSLLSIAGLSGFCQVSIPLGMRNGLPVSVSLVARHGADHFLLNVVEELYQTL 425

BLAST of PI0006193 vs. ExPASy Swiss-Prot
Match: Q9MUK5 (Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum OX=3888 GN=TOC64 PE=1 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 9.7e-122
Identity = 213/423 (50.35%), Postives = 293/423 (69.27%), Query Frame = 0

Query: 4   QHYGAFMEKFLL----QPSSPNDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPAN 63
           + +GAF+ K  L     P+ P    PL+ L FA+ DIFD++GHV+ FG+PEW RTH PA+
Sbjct: 35  EDFGAFLLKLKLLPPPPPAPPKAPHPLSSLNFAISDIFDIEGHVSTFGHPEWARTHEPAS 94

Query: 64  HTAPTVSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV 123
            TA  VS ++  GATCIG T++DE+AY I+GEN H+GTP NP   +RVPGGSSSG+AVAV
Sbjct: 95  STASAVSALVESGATCIGTTVVDELAYGISGENKHFGTPTNPAVPNRVPGGSSSGAAVAV 154

Query: 124 GANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVV 183
            AN VDFSLG DT G VRVPA +CGILGFRPSHGAVS  G++P++ S DTVGWFA+DP V
Sbjct: 155 AANFVDFSLGVDTSGGVRVPAGFCGILGFRPSHGAVSHVGIIPVSTSLDTVGWFAKDPDV 214

Query: 184 LKKVGHLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVS 243
           L++VGH+LLQ P V  + P Q++IA+DCF+  ++P +R +Q  + + +KLFG  ++K ++
Sbjct: 215 LRRVGHILLQAPFVMQRNPRQIIIADDCFQHLNVPLDRTSQVVIKATEKLFGKQVLKHIN 274

Query: 244 LGNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHL 303
             +Y+  KV SLK   I+ + G   S  SL  LA  +Q LQR+EF+  H EW+    P L
Sbjct: 275 FEDYISSKVSSLKACSIQKSNGVLKS-SSLKLLANVMQSLQRHEFEHTHSEWMSIVKPDL 334

Query: 304 GPGISERVSEAMRATDENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNTDVS 363
            P +S ++ E    ++  I+   S++ ELR A+ +LL+D GVL IPTV  P PKL     
Sbjct: 335 HPAVSAQLHEKFEVSELEIENSKSVRSELRVAVNSLLKDEGVLVIPTVADPPPKLGGKEF 394

Query: 364 KLHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNT 423
             HD++++A SLLSIA +SG CQV++PLG ++  PVS+SL+A HG D FLL+ + ++Y  
Sbjct: 395 LSHDYQSRALSLLSIASISGCCQVTVPLGFFDKNPVSVSLIARHGGDRFLLDTLKTMYTV 454

BLAST of PI0006193 vs. ExPASy Swiss-Prot
Match: Q9LVH5 (Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP64 PE=1 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 2.8e-121
Identity = 208/423 (49.17%), Postives = 292/423 (69.03%), Query Frame = 0

Query: 4   QHYGAFMEKFLL----QPSSPNDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPAN 63
           + +GAF++K +L    QP+ P    PLTGLTFAV D+FD+ G+VTGFG+P+W+RTH  A+
Sbjct: 32  EDFGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAAS 91

Query: 64  HTAPTVSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV 123
            T+P VST++ GGATC+GKT++DE A+SI+GEN HY +P NP A  R+PGG+ SG+AVAV
Sbjct: 92  STSPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAV 151

Query: 124 GANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVV 183
             N VDF+LG DT G VRVPA YCG+LGF+ S+GA+S +G++P++ S D+VGWFARDP  
Sbjct: 152 ATNAVDFALGIDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSVGWFARDPNT 211

Query: 184 LKKVGHLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVS 243
           L++VGH+LLQ P    + P Q+++A+DCF+L  IP +R+TQ    S +KLFG  L+K  +
Sbjct: 212 LRRVGHVLLQLPFATQRNPRQIILADDCFQLLKIPVDRITQVVTKSAEKLFGRQLLKHQN 271

Query: 244 LGNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHL 303
           L  Y E KVPSLK F       N   + +   LA  +QLLQR+EF  NH +W+ T  P +
Sbjct: 272 LETYFETKVPSLKEFARTKAIANT-KVSTSRLLANVMQLLQRHEFLQNHGDWINTVKPAI 331

Query: 304 GPGISERVSEAMRATDENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNTDVS 363
            P I  +V E    T+E  +  ++I+ E R A+ +LL+D G+L IPT+P   PKL +   
Sbjct: 332 DPVILSQVCENPELTNEETENLNAIRNETRVAIGSLLKDDGILVIPTLPAVPPKLGSKEI 391

Query: 364 KLHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNT 423
              D++ +A SLLSIA +SG CQV++PLG +   P+S+S +  HG D FLL+ V ++Y +
Sbjct: 392 TSEDYQNRASSLLSIASISGCCQVTVPLGHHEKCPISVSFIGRHGGDRFLLDTVQTMYPS 451

BLAST of PI0006193 vs. ExPASy Swiss-Prot
Match: F4KCL7 (Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OM64 PE=1 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 5.5e-109
Identity = 200/424 (47.17%), Postives = 283/424 (66.75%), Query Frame = 0

Query: 4   QHYGAFMEKFLL----QPSSPNDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPAN 63
           + +GAF+++F L     P  P  +  L+GLTF++ D FD+  ++TGFG P+W +TH  A 
Sbjct: 45  EDFGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAE 104

Query: 64  HTAPTVSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV 123
            TA  V+T+L+ GATC+GKTIMDE+ + I GEN HYGTP NP   D VPGG SSGSAV+V
Sbjct: 105 KTAVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSV 164

Query: 124 GANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVV 183
           GA LVDFSLG DT G VRVPA++CGILGFRPS G VS+ GV+P +QS +TVGWFA DP V
Sbjct: 165 GAELVDFSLGIDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETVGWFASDPSV 224

Query: 184 LKKVGHLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVS 243
           L +VGH LL    V H++   ++ A+D F+LS IP ++  Q    +++ L G    K V+
Sbjct: 225 LCQVGHALLNLSAVTHRRQRSLIFADDLFELSDIPKQKSVQVVRKAIENLSGYKTPKHVN 284

Query: 244 LGNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHL 303
           +G YV   VPSL  F  E +  +++S  +L AL+  +  +QR+EFK NHEEW +T    L
Sbjct: 285 VGQYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHEEWWQTCKSFL 344

Query: 304 GPGISERVSEAMRATDENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNTDVS 363
           GP  S  V  A+++ +E+I   + +K E+R  + +LL++ G+L IPTV  P P+LNT  +
Sbjct: 345 GPRFSNDVVTALKSKNESIKSLYRVKNEMRATIQSLLKEDGILVIPTVADPPPRLNTKRN 404

Query: 364 K-LHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYN 423
           K L++F  + ++L  IA +SG CQV+IPLG +   P+S+SL+  +G D FLL+    +Y 
Sbjct: 405 KSLNEFLDRTYALSCIASMSGCCQVTIPLGEHGDRPISVSLLTYYGGDKFLLDTTLDVYA 464

BLAST of PI0006193 vs. ExPASy TrEMBL
Match: A0A1S3BPN7 (amidase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492180 PE=3 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 1.7e-238
Identity = 417/428 (97.43%), Postives = 422/428 (98.60%), Query Frame = 0

Query: 1   MAVQHYGAFMEKFLLQPSSPNDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60
           MAVQ YGAFMEKFLLQPSSP+DQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH
Sbjct: 1   MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPTVSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120
           TAPTVSTILRGGATCIG+TIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVL 180
           ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVV 
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVF 180

Query: 181 KKVGHLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVSL 240
           KKVG LLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQV+L
Sbjct: 181 KKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNL 240

Query: 241 GNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG 300
           GNYVEEKVPSLK FMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG
Sbjct: 241 GNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG 300

Query: 301 PGISERVSEAMRATDENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNTDVSK 360
           PGISERVSEAMRATDENIDLCHSIKIELR+ALAALLEDFGVLAIPTVPGP PKLNTDVS+
Sbjct: 301 PGISERVSEAMRATDENIDLCHSIKIELRRALAALLEDFGVLAIPTVPGPPPKLNTDVSE 360

Query: 361 LHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTL 420
           LHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTL
Sbjct: 361 LHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTL 420

Query: 421 -EEVKASF 428
            EEVKASF
Sbjct: 421 EEEVKASF 427

BLAST of PI0006193 vs. ExPASy TrEMBL
Match: A0A0A0M1K1 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G570110 PE=3 SV=1)

HSP 1 Score: 832.8 bits (2150), Expect = 6.5e-238
Identity = 415/428 (96.96%), Postives = 420/428 (98.13%), Query Frame = 0

Query: 1   MAVQHYGAFMEKFLLQPSSPNDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60
           MAVQ YGAFMEKFLLQPSSP+DQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH
Sbjct: 1   MAVQCYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPTVSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120
           TAPTVSTILRGGATCIG+TIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVL 180
           ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQS DTVGWFARDPVVL
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSMDTVGWFARDPVVL 180

Query: 181 KKVGHLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVSL 240
           KKVG LLLQQPEVEHQKP QVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGH IK+VSL
Sbjct: 181 KKVGWLLLQQPEVEHQKPRQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHPIKEVSL 240

Query: 241 GNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG 300
           GNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG
Sbjct: 241 GNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG 300

Query: 301 PGISERVSEAMRATDENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNTDVSK 360
           PGISERVSEAMRATDENIDLCHSI+IELRKALAALLEDFGVLAIPTVPGP PKLNTDVS+
Sbjct: 301 PGISERVSEAMRATDENIDLCHSIRIELRKALAALLEDFGVLAIPTVPGPPPKLNTDVSE 360

Query: 361 LHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTL 420
           L DFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTL
Sbjct: 361 LQDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTL 420

Query: 421 -EEVKASF 428
            EEVKASF
Sbjct: 421 EEEVKASF 428

BLAST of PI0006193 vs. ExPASy TrEMBL
Match: A0A5D3CG55 (Amidase 1-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003790 PE=4 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 9.4e-237
Identity = 417/432 (96.53%), Postives = 422/432 (97.69%), Query Frame = 0

Query: 1   MAVQHYGAFMEKFLLQPSSPNDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60
           MAVQ YGAFMEKFLLQPSSP+DQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH
Sbjct: 46  MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 105

Query: 61  TAPTVSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120
           TAPTVSTILRGGATCIG+TIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG
Sbjct: 106 TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 165

Query: 121 ANLVDFSL----GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARD 180
           ANLVDFSL    GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARD
Sbjct: 166 ANLVDFSLGTFVGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARD 225

Query: 181 PVVLKKVGHLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK 240
           PVV KKVG LLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK
Sbjct: 226 PVVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK 285

Query: 241 QVSLGNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYN 300
           QV+LGNYVEEKVPSLK FMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYN
Sbjct: 286 QVNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYN 345

Query: 301 PHLGPGISERVSEAMRATDENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNT 360
           PHLGPGISERVSEAMRATDENIDLCHSIKIELR+ALAALLEDFGVLAIPTVPGP PKLNT
Sbjct: 346 PHLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLEDFGVLAIPTVPGPPPKLNT 405

Query: 361 DVSKLHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSL 420
           DVS+LHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSL
Sbjct: 406 DVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSL 465

Query: 421 YNTL-EEVKASF 428
           YNTL EEVKASF
Sbjct: 466 YNTLEEEVKASF 476

BLAST of PI0006193 vs. ExPASy TrEMBL
Match: A0A1S4DYH0 (amidase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492180 PE=3 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 3.6e-236
Identity = 417/437 (95.42%), Postives = 422/437 (96.57%), Query Frame = 0

Query: 1   MAVQHYGAFMEKFLLQPSSPNDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60
           MAVQ YGAFMEKFLLQPSSP+DQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH
Sbjct: 1   MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPTVSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120
           TAPTVSTILRGGATCIG+TIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVL 180
           ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVV 
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVF 180

Query: 181 KKVGHLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVSL 240
           KKVG LLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQV+L
Sbjct: 181 KKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNL 240

Query: 241 GNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG 300
           GNYVEEKVPSLK FMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG
Sbjct: 241 GNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG 300

Query: 301 PGISERVSEAMRATDENIDLCHSIKIELRKALAALLE---------DFGVLAIPTVPGPA 360
           PGISERVSEAMRATDENIDLCHSIKIELR+ALAALLE         DFGVLAIPTVPGP 
Sbjct: 301 PGISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPP 360

Query: 361 PKLNTDVSKLHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLN 420
           PKLNTDVS+LHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLN
Sbjct: 361 PKLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLN 420

Query: 421 VVHSLYNTL-EEVKASF 428
           VVHSLYNTL EEVKASF
Sbjct: 421 VVHSLYNTLEEEVKASF 436

BLAST of PI0006193 vs. ExPASy TrEMBL
Match: A0A5A7U6R8 (Amidase 1-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold761G00050 PE=4 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 7.9e-236
Identity = 416/432 (96.30%), Postives = 421/432 (97.45%), Query Frame = 0

Query: 1   MAVQHYGAFMEKFLLQPSSPNDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60
           MAVQ YGAFMEKFLLQPSSP+DQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH
Sbjct: 46  MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 105

Query: 61  TAPTVSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120
           TAPTVSTILRGGATCIG+TIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG
Sbjct: 106 TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 165

Query: 121 ANLVDFSL----GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARD 180
           ANLVDFSL    GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARD
Sbjct: 166 ANLVDFSLGTFVGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARD 225

Query: 181 PVVLKKVGHLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK 240
           PVV KKVG LLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK
Sbjct: 226 PVVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK 285

Query: 241 QVSLGNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYN 300
           QV+LGNYVEEKVPSLK FMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYN
Sbjct: 286 QVNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYN 345

Query: 301 PHLGPGISERVSEAMRATDENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNT 360
           PHLGPGISERVSEAMRATDENIDLCHSIKIELR+ALAALLEDFGVLAIPTVPGP PKLNT
Sbjct: 346 PHLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLEDFGVLAIPTVPGPPPKLNT 405

Query: 361 DVSKLHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSL 420
           DVS+LHDFRAKAFSLLSIAGVSGFCQVSIPL LYNGLPVSISLVANHGSDGFLLNVVHSL
Sbjct: 406 DVSELHDFRAKAFSLLSIAGVSGFCQVSIPLRLYNGLPVSISLVANHGSDGFLLNVVHSL 465

Query: 421 YNTL-EEVKASF 428
           YNTL EEVKASF
Sbjct: 466 YNTLEEEVKASF 476

BLAST of PI0006193 vs. NCBI nr
Match: XP_008450658.1 (PREDICTED: amidase 1-like isoform X2 [Cucumis melo])

HSP 1 Score: 834.7 bits (2155), Expect = 3.5e-238
Identity = 417/428 (97.43%), Postives = 422/428 (98.60%), Query Frame = 0

Query: 1   MAVQHYGAFMEKFLLQPSSPNDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60
           MAVQ YGAFMEKFLLQPSSP+DQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH
Sbjct: 1   MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPTVSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120
           TAPTVSTILRGGATCIG+TIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVL 180
           ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVV 
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVF 180

Query: 181 KKVGHLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVSL 240
           KKVG LLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQV+L
Sbjct: 181 KKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNL 240

Query: 241 GNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG 300
           GNYVEEKVPSLK FMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG
Sbjct: 241 GNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG 300

Query: 301 PGISERVSEAMRATDENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNTDVSK 360
           PGISERVSEAMRATDENIDLCHSIKIELR+ALAALLEDFGVLAIPTVPGP PKLNTDVS+
Sbjct: 301 PGISERVSEAMRATDENIDLCHSIKIELRRALAALLEDFGVLAIPTVPGPPPKLNTDVSE 360

Query: 361 LHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTL 420
           LHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTL
Sbjct: 361 LHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTL 420

Query: 421 -EEVKASF 428
            EEVKASF
Sbjct: 421 EEEVKASF 427

BLAST of PI0006193 vs. NCBI nr
Match: XP_004135624.1 (amidase 1 [Cucumis sativus] >KGN66086.1 hypothetical protein Csa_006860 [Cucumis sativus])

HSP 1 Score: 832.8 bits (2150), Expect = 1.3e-237
Identity = 415/428 (96.96%), Postives = 420/428 (98.13%), Query Frame = 0

Query: 1   MAVQHYGAFMEKFLLQPSSPNDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60
           MAVQ YGAFMEKFLLQPSSP+DQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH
Sbjct: 1   MAVQCYGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPTVSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120
           TAPTVSTILRGGATCIG+TIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVL 180
           ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQS DTVGWFARDPVVL
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSMDTVGWFARDPVVL 180

Query: 181 KKVGHLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVSL 240
           KKVG LLLQQPEVEHQKP QVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGH IK+VSL
Sbjct: 181 KKVGWLLLQQPEVEHQKPRQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHPIKEVSL 240

Query: 241 GNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG 300
           GNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG
Sbjct: 241 GNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG 300

Query: 301 PGISERVSEAMRATDENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNTDVSK 360
           PGISERVSEAMRATDENIDLCHSI+IELRKALAALLEDFGVLAIPTVPGP PKLNTDVS+
Sbjct: 301 PGISERVSEAMRATDENIDLCHSIRIELRKALAALLEDFGVLAIPTVPGPPPKLNTDVSE 360

Query: 361 LHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTL 420
           L DFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTL
Sbjct: 361 LQDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNTL 420

Query: 421 -EEVKASF 428
            EEVKASF
Sbjct: 421 EEEVKASF 428

BLAST of PI0006193 vs. NCBI nr
Match: TYK10232.1 (amidase 1-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 828.9 bits (2140), Expect = 1.9e-236
Identity = 417/432 (96.53%), Postives = 422/432 (97.69%), Query Frame = 0

Query: 1   MAVQHYGAFMEKFLLQPSSPNDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60
           MAVQ YGAFMEKFLLQPSSP+DQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH
Sbjct: 46  MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 105

Query: 61  TAPTVSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120
           TAPTVSTILRGGATCIG+TIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG
Sbjct: 106 TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 165

Query: 121 ANLVDFSL----GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARD 180
           ANLVDFSL    GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARD
Sbjct: 166 ANLVDFSLGTFVGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARD 225

Query: 181 PVVLKKVGHLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK 240
           PVV KKVG LLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK
Sbjct: 226 PVVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK 285

Query: 241 QVSLGNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYN 300
           QV+LGNYVEEKVPSLK FMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYN
Sbjct: 286 QVNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYN 345

Query: 301 PHLGPGISERVSEAMRATDENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNT 360
           PHLGPGISERVSEAMRATDENIDLCHSIKIELR+ALAALLEDFGVLAIPTVPGP PKLNT
Sbjct: 346 PHLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLEDFGVLAIPTVPGPPPKLNT 405

Query: 361 DVSKLHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSL 420
           DVS+LHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSL
Sbjct: 406 DVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSL 465

Query: 421 YNTL-EEVKASF 428
           YNTL EEVKASF
Sbjct: 466 YNTLEEEVKASF 476

BLAST of PI0006193 vs. NCBI nr
Match: XP_016901018.1 (PREDICTED: amidase 1-like isoform X1 [Cucumis melo])

HSP 1 Score: 827.0 bits (2135), Expect = 7.4e-236
Identity = 417/437 (95.42%), Postives = 422/437 (96.57%), Query Frame = 0

Query: 1   MAVQHYGAFMEKFLLQPSSPNDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60
           MAVQ YGAFMEKFLLQPSSP+DQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH
Sbjct: 1   MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60

Query: 61  TAPTVSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120
           TAPTVSTILRGGATCIG+TIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG
Sbjct: 61  TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120

Query: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVL 180
           ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVV 
Sbjct: 121 ANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVF 180

Query: 181 KKVGHLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVSL 240
           KKVG LLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQV+L
Sbjct: 181 KKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVNL 240

Query: 241 GNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG 300
           GNYVEEKVPSLK FMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG
Sbjct: 241 GNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLG 300

Query: 301 PGISERVSEAMRATDENIDLCHSIKIELRKALAALLE---------DFGVLAIPTVPGPA 360
           PGISERVSEAMRATDENIDLCHSIKIELR+ALAALLE         DFGVLAIPTVPGP 
Sbjct: 301 PGISERVSEAMRATDENIDLCHSIKIELRRALAALLEVLYLSRMLHDFGVLAIPTVPGPP 360

Query: 361 PKLNTDVSKLHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLN 420
           PKLNTDVS+LHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLN
Sbjct: 361 PKLNTDVSELHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLN 420

Query: 421 VVHSLYNTL-EEVKASF 428
           VVHSLYNTL EEVKASF
Sbjct: 421 VVHSLYNTLEEEVKASF 436

BLAST of PI0006193 vs. NCBI nr
Match: KAA0050880.1 (amidase 1-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 825.9 bits (2132), Expect = 1.6e-235
Identity = 416/432 (96.30%), Postives = 421/432 (97.45%), Query Frame = 0

Query: 1   MAVQHYGAFMEKFLLQPSSPNDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 60
           MAVQ YGAFMEKFLLQPSSP+DQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH
Sbjct: 46  MAVQ-YGAFMEKFLLQPSSPSDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANH 105

Query: 61  TAPTVSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 120
           TAPTVSTILRGGATCIG+TIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG
Sbjct: 106 TAPTVSTILRGGATCIGRTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVG 165

Query: 121 ANLVDFSL----GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARD 180
           ANLVDFSL    GTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARD
Sbjct: 166 ANLVDFSLGTFVGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARD 225

Query: 181 PVVLKKVGHLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK 240
           PVV KKVG LLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK
Sbjct: 226 PVVFKKVGLLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIK 285

Query: 241 QVSLGNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYN 300
           QV+LGNYVEEKVPSLK FMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYN
Sbjct: 286 QVNLGNYVEEKVPSLKQFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYN 345

Query: 301 PHLGPGISERVSEAMRATDENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNT 360
           PHLGPGISERVSEAMRATDENIDLCHSIKIELR+ALAALLEDFGVLAIPTVPGP PKLNT
Sbjct: 346 PHLGPGISERVSEAMRATDENIDLCHSIKIELRRALAALLEDFGVLAIPTVPGPPPKLNT 405

Query: 361 DVSKLHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSL 420
           DVS+LHDFRAKAFSLLSIAGVSGFCQVSIPL LYNGLPVSISLVANHGSDGFLLNVVHSL
Sbjct: 406 DVSELHDFRAKAFSLLSIAGVSGFCQVSIPLRLYNGLPVSISLVANHGSDGFLLNVVHSL 465

Query: 421 YNTL-EEVKASF 428
           YNTL EEVKASF
Sbjct: 466 YNTLEEEVKASF 476

BLAST of PI0006193 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 540.4 bits (1391), Expect = 1.3e-153
Identity = 270/415 (65.06%), Postives = 325/415 (78.31%), Query Frame = 0

Query: 6   YGAFMEKFLLQPSSPNDQLP-LTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHTAPT 65
           +GAF+EK  + P+S +   P L GLTFA+KDIFD++G VTGFGNP+WLRTH  A  TAP 
Sbjct: 7   FGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAPV 66

Query: 66  VSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVGANLV 125
           VS++L  GAT +G TIMDEMAYSINGEN HYGTP+NP A DRVPGGSSSGSAVAV A LV
Sbjct: 67  VSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARLV 126

Query: 126 DFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVLKKVG 185
           DFS+GTDTGGSVRVPASYCGI GFRPSHGAVST G+ PMAQSFDTVGWFARD   LK+VG
Sbjct: 127 DFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARDTATLKRVG 186

Query: 186 HLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGH-LIKQVSLGNY 245
            +LLQQ  +   +P+Q++IA+DCFKL S+P + L Q  V SV+K FGG+ ++K+V+LG Y
Sbjct: 187 CVLLQQHHLNPIEPSQLIIADDCFKLCSVPHDLLVQPLVGSVEKSFGGNTVVKKVNLGEY 246

Query: 246 VEEKVPSLKHFMIEGN--AGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHLGP 305
           + + VPSLKHFM   +     E  IPSL AL+ S++LLQR+EFKINH  W+ +  P  GP
Sbjct: 247 IGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWISSVKPEFGP 306

Query: 306 GISERVSEAMRATDENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNTDVSKL 365
           GISER+ EA+R +DE ID C S+K EL  AL+ LL + GVL IPTVPGP P L  +V+ L
Sbjct: 307 GISERIEEAIRTSDEKIDHCRSVKSELITALSTLLGEKGVLVIPTVPGPPPHLQANVAAL 366

Query: 366 HDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSL 417
             FR++AFSLLSIAGVSGFCQVSIPLGL+  LPVS+SLVA +GSDGFLL++V SL
Sbjct: 367 ESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSLVDSL 421

BLAST of PI0006193 vs. TAIR 10
Match: AT3G17970.1 (translocon at the outer membrane of chloroplasts 64-III )

HSP 1 Score: 436.8 bits (1122), Expect = 2.0e-122
Identity = 208/423 (49.17%), Postives = 292/423 (69.03%), Query Frame = 0

Query: 4   QHYGAFMEKFLL----QPSSPNDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPAN 63
           + +GAF++K +L    QP+ P    PLTGLTFAV D+FD+ G+VTGFG+P+W+RTH  A+
Sbjct: 32  EDFGAFIDKLMLLPPPQPAPPKAPHPLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAAS 91

Query: 64  HTAPTVSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV 123
            T+P VST++ GGATC+GKT++DE A+SI+GEN HY +P NP A  R+PGG+ SG+AVAV
Sbjct: 92  STSPVVSTLVEGGATCVGKTVVDEFAFSISGENKHYDSPTNPAAPTRIPGGACSGAAVAV 151

Query: 124 GANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVV 183
             N VDF+LG DT G VRVPA YCG+LGF+ S+GA+S +G++P++ S D+VGWFARDP  
Sbjct: 152 ATNAVDFALGIDTVGGVRVPAGYCGVLGFKSSYGAISNTGIIPVSSSLDSVGWFARDPNT 211

Query: 184 LKKVGHLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVS 243
           L++VGH+LLQ P    + P Q+++A+DCF+L  IP +R+TQ    S +KLFG  L+K  +
Sbjct: 212 LRRVGHVLLQLPFATQRNPRQIILADDCFQLLKIPVDRITQVVTKSAEKLFGRQLLKHQN 271

Query: 244 LGNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHL 303
           L  Y E KVPSLK F       N   + +   LA  +QLLQR+EF  NH +W+ T  P +
Sbjct: 272 LETYFETKVPSLKEFARTKAIANT-KVSTSRLLANVMQLLQRHEFLQNHGDWINTVKPAI 331

Query: 304 GPGISERVSEAMRATDENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNTDVS 363
            P I  +V E    T+E  +  ++I+ E R A+ +LL+D G+L IPT+P   PKL +   
Sbjct: 332 DPVILSQVCENPELTNEETENLNAIRNETRVAIGSLLKDDGILVIPTLPAVPPKLGSKEI 391

Query: 364 KLHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYNT 423
              D++ +A SLLSIA +SG CQV++PLG +   P+S+S +  HG D FLL+ V ++Y +
Sbjct: 392 TSEDYQNRASSLLSIASISGCCQVTVPLGHHEKCPISVSFIGRHGGDRFLLDTVQTMYPS 451

BLAST of PI0006193 vs. TAIR 10
Match: AT5G09420.1 (translocon at the outer membrane of chloroplasts 64-V )

HSP 1 Score: 396.0 bits (1016), Expect = 3.9e-110
Identity = 200/424 (47.17%), Postives = 283/424 (66.75%), Query Frame = 0

Query: 4   QHYGAFMEKFLL----QPSSPNDQLPLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPAN 63
           + +GAF+++F L     P  P  +  L+GLTF++ D FD+  ++TGFG P+W +TH  A 
Sbjct: 45  EDFGAFLDRFELLPFPPPPPPAAKQSLSGLTFSISDAFDVKDYITGFGCPQWKKTHEAAE 104

Query: 64  HTAPTVSTILRGGATCIGKTIMDEMAYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAV 123
            TA  V+T+L+ GATC+GKTIMDE+ + I GEN HYGTP NP   D VPGG SSGSAV+V
Sbjct: 105 KTAVVVTTLLKNGATCVGKTIMDELGFGIIGENKHYGTPINPLMPDNVPGGCSSGSAVSV 164

Query: 124 GANLVDFSLGTDTGGSVRVPASYCGILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVV 183
           GA LVDFSLG DT G VRVPA++CGILGFRPS G VS+ GV+P +QS +TVGWFA DP V
Sbjct: 165 GAELVDFSLGIDTTGGVRVPAAFCGILGFRPSQGTVSSVGVLPNSQSLETVGWFASDPSV 224

Query: 184 LKKVGHLLLQQPEVEHQKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVS 243
           L +VGH LL    V H++   ++ A+D F+LS IP ++  Q    +++ L G    K V+
Sbjct: 225 LCQVGHALLNLSAVTHRRQRSLIFADDLFELSDIPKQKSVQVVRKAIENLSGYKTPKHVN 284

Query: 244 LGNYVEEKVPSLKHFMIEGNAGNEHSIPSLAALARSLQLLQRYEFKINHEEWVRTYNPHL 303
           +G YV   VPSL  F  E +  +++S  +L AL+  +  +QR+EFK NHEEW +T    L
Sbjct: 285 VGQYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHEEWWQTCKSFL 344

Query: 304 GPGISERVSEAMRATDENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNTDVS 363
           GP  S  V  A+++ +E+I   + +K E+R  + +LL++ G+L IPTV  P P+LNT  +
Sbjct: 345 GPRFSNDVVTALKSKNESIKSLYRVKNEMRATIQSLLKEDGILVIPTVADPPPRLNTKRN 404

Query: 364 K-LHDFRAKAFSLLSIAGVSGFCQVSIPLGLYNGLPVSISLVANHGSDGFLLNVVHSLYN 423
           K L++F  + ++L  IA +SG CQV+IPLG +   P+S+SL+  +G D FLL+    +Y 
Sbjct: 405 KSLNEFLDRTYALSCIASMSGCCQVTIPLGEHGDRPISVSLLTYYGGDKFLLDTTLDVYA 464

BLAST of PI0006193 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 114.8 bits (286), Expect = 1.7e-25
Identity = 121/430 (28.14%), Postives = 177/430 (41.16%), Query Frame = 0

Query: 25  PLTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHTAPTVSTILRGGATCIGKTIMDEM 84
           PL G+   VKD     G  +   +       PP + TA  V  I   G   +GKT MDE 
Sbjct: 107 PLAGVLIGVKDNICTQGMPSTAASRILEHYRPPFDATA--VKKIKELGGIVVGKTNMDEF 166

Query: 85  AYSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVGANLVDFSLGTDTGGSVRVPASYCG 144
                 E   +    NP    RVPGGSS GSA AV A     SLG+DTGGSVR PAS+CG
Sbjct: 167 GMGSTTEASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCG 226

Query: 145 ILGFRPSHGAVSTSGVVPMAQSFDTVGWFARDPVVLKKVGHLLLQQPEVEHQKPTQVLIA 204
           ++G +P++G VS  G++  A S D +G F      +   G LL      +    T     
Sbjct: 227 VVGLKPTYGRVSRFGLMAYASSLDVIGCFGS---TVADAGMLLHAISGYDRFDSTSSKQD 286

Query: 205 EDCFKLSSIPSERLTQAFVNSVKKLFGGHLIKQVSLGNYVEEKVPS-----LKHFMIEGN 264
              F+   +  +      +N VK      +I++ +L + V+  V S       H    G 
Sbjct: 287 VPEFQSQFLSVDHFESKPLNGVKV----GIIRE-TLEDGVDSGVRSATQEAASHLEALGC 346

Query: 265 AGNEHSIPSLA---------ALARSLQLLQRYE-----FKINHEEWVRTYNPHLGPGISE 324
              E S+PS +         A + S   L RY+      ++  EE  + Y    G G   
Sbjct: 347 ILTEVSLPSFSLGLPAYYVIASSESSSNLSRYDGVRYGNQVMAEELNKLYECSRGEGFGG 406

Query: 325 RV-------SEAMRA--TDENIDLCHSIKIELRKALAALLEDFGVLAIPTVPGPAPKLNT 384
            V       + A+ A   D        ++  +RK   A LE   +L  P  P  A K+  
Sbjct: 407 EVKMRILMGTYALSAGYYDAYYKRAQQVRTLIRKDFKAALEQNDILISPAAPSAAYKIGE 466

Query: 385 DVSKLHDFRAKAFSLLSI-AGVSGFCQVSIPLGLY----NGLPVSISLVANHGSDGFLLN 422
              K       A  ++++   ++G   + +P GL     +GLPV + ++     +  LL 
Sbjct: 467 --KKDDPLAMYAGDIMTVNVNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLK 524

BLAST of PI0006193 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 96.7 bits (239), Expect = 4.9e-20
Identity = 54/146 (36.99%), Postives = 74/146 (50.68%), Query Frame = 0

Query: 26  LTGLTFAVKDIFDMDGHVTGFGNPEWLRTHPPANHTAPTVSTILRGGATCIGKTIMDEMA 85
           L G+   +KD  D   H T  G   WL         +  VS +   GA  +GK  M E+ 
Sbjct: 197 LDGIFVTIKDDIDCLPHPTN-GGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKANMHELG 256

Query: 86  YSINGENFHYGTPQNPCASDRVPGGSSSGSAVAVGANLVDFSLGTDTGGSVRVPASYCGI 145
               G N +YGT +NP    R  GGSSSGSA  V A L   +LGTD GGSVR+P++ CGI
Sbjct: 257 MGTTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAGLCSAALGTDGGGSVRIPSALCGI 316

Query: 146 LGFRPSHGAVSTSGVVPMAQSFDTVG 172
            G + ++G    +G +    + + +G
Sbjct: 317 TGLKTTYGRTDMTGSLCEGGTVEIIG 341

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FR371.8e-15265.06Amidase 1 OS=Arabidopsis thaliana OX=3702 GN=AMI1 PE=1 SV=1[more]
Q7XTK36.7e-13959.38Amidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMI1 PE=1 SV=1[more]
Q9MUK59.7e-12250.35Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum OX=3888 GN=... [more]
Q9LVH52.8e-12149.17Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP6... [more]
F4KCL75.5e-10947.17Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OM64... [more]
Match NameE-valueIdentityDescription
A0A1S3BPN71.7e-23897.43amidase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492180 PE=3 SV=1[more]
A0A0A0M1K16.5e-23896.96Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G570110 PE=... [more]
A0A5D3CG559.4e-23796.53Amidase 1-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S4DYH03.6e-23695.42amidase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492180 PE=3 SV=1[more]
A0A5A7U6R87.9e-23696.30Amidase 1-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
Match NameE-valueIdentityDescription
XP_008450658.13.5e-23897.43PREDICTED: amidase 1-like isoform X2 [Cucumis melo][more]
XP_004135624.11.3e-23796.96amidase 1 [Cucumis sativus] >KGN66086.1 hypothetical protein Csa_006860 [Cucumis... [more]
TYK10232.11.9e-23696.53amidase 1-like isoform X2 [Cucumis melo var. makuwa][more]
XP_016901018.17.4e-23695.42PREDICTED: amidase 1-like isoform X1 [Cucumis melo][more]
KAA0050880.11.6e-23596.30amidase 1-like isoform X2 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G08980.11.3e-15365.06amidase 1 [more]
AT3G17970.12.0e-12249.17translocon at the outer membrane of chloroplasts 64-III [more]
AT5G09420.13.9e-11047.17translocon at the outer membrane of chloroplasts 64-V [more]
AT3G25660.11.7e-2528.14Amidase family protein [more]
AT5G64440.14.9e-2036.99fatty acid amide hydrolase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 22..410
e-value: 5.2E-60
score: 203.7
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 12..421
e-value: 7.0E-93
score: 314.1
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 14..416
NoneNo IPR availablePANTHERPTHR46310:SF7AMIDASE 1coord: 4..423
NoneNo IPR availablePANTHERPTHR46310AMIDASE 1coord: 4..423
IPR020556Amidase, conserved sitePROSITEPS00571AMIDASEScoord: 109..140

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0006193.1PI0006193.1mRNA