PI0005633 (gene) Melon (PI 482460) v1

Overview
NamePI0005633
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionglutathione hydrolase 1
Locationchr11: 8643897 .. 8648569 (-)
RNA-Seq ExpressionPI0005633
SyntenyPI0005633
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACTATAATAAGGTCATGTCAATTATCGTTGAACTAAGTTCGTTTTGGAAATTAAACATTAGTAGTTGAGTGTTTGTTAATAATAGCCAAATACTTTTTTATTTGACTATTTATCATTTTGGTATGTTTTAATAGGAGATTTTGCTTTTGAAATTTTGTAAATTATTGTAAGATTTTATGTTTTAACTTAAAATTTTGATTTTGATTTTGATTTTCACTATTTTCAAATATAACAAAATGAATCAACATATTTACAAAATATATCAAAACATCAAAGTCCATTTACAATGAATTGCGATAGATCAAGATACACTACTATCTACGATGAACATCGAGAGTATTTTTTTGAACTATTTTTAAAAATTAATTAAAGGTATTTTGAAACTTCAATGAACCTTTTTTATACAAAATGGCACGCTTTTTTTATTAGTTAGTCTTTTACTATTTATTGTCCATCTCTTATCGTCTTTAATAACTAGTCTCTCTACCGAACAAGGTTTTCCCATATCAAAATCTACCTTGCAAATTCTACTACACAATAATAAAACTTCAAATAAATAAATAAATAATCAATAAACACACCTATTCAATTTCATATTTTATTGCATGTTTTTCCACTACGTGAGCATTTGTGCCAATATTGTATATACTGATCTTGATAATTATTTACTCCTTTTTTTTCTTTATAAATACTGAATCGATTAGAAGAGTATAGTATTGGCTAAGAAATTAATTAAATCAATAGTAGTTTGCATTTGAGAGGTTACAACAAAACAAAATGGTGATGGTGATGCATTGGTTAATAAGCGTTGTTGTAATTTCTACTGTGTTGTTATTGTTGCCTTTCACCTCCATAGCTCGACCTGAAGTTATTGAAGCAAGTCTTGGCGCTGTTGCTGCAGATGATCATCGATGCTCGAAAATCGGTGTGTATGTTCTACAAAAATTAAAAGGAAATGCTGTTGATGCGGCTGTGGCTACAGCTCTTTGTCAAGGGGTTGTTAGTCCAGCATCAAGTGGCATTGGTGGAGGAGGCTTTATGTTGATCAGATTGGCCAATGGAGAGTCTCAAGTTTTTGATATGAGGGAGATTGCACCTTTGAAAGCTCATGAGGTTCTCTACGTTTTACTTCACTTTTTTTCCATTATTTTATCTTGAATTGTCGAATTTAATCTTTTTTTTCTTTCCCTAAAATACCTTCGAAATATATGTTACGTGTTTCAACTTTCATAAACATTTTAAAACTACATAAATTATATATACGCTTAAAATTTGTGTAATGATGACCTAAAGCAATATGGTAATTCAAATATATGTGTGTGTATGTATGGATCTATCTATATGTATACATCTATTAATGTTTCCTCTTCATTTGGTTGGCTCTATATACTTTCTTGTGTTTATCTCTAGCTTTTTGCTTGGGACATCATCTTGTACTTTCTTACTTTTTCTTAATATATTTTGAAGTAGAGATGATAAAGTTGTTCACCGAGCTAGTGAGGATGTAGCTTGGTTGCAACTACTGACCCCGTCTCGTATCTTTCTAATTAGAGAAAAAATTGCAATTTTTCAGAGTATAAATAACAAAGTTCAAAGGTGTATACCTTTTTTTTATATAATTTAAACTTTTTTAATCAATCTTTCTCATTTAATTTTCAAAAATAGCATAATATTGAAACTATTTACAAAATACAGAATAATTCATTAACTTGAGTAGTTCATTTTTTTTTTTTACATTTTGTAAACAGTTTTTTTTTTTTCCGTGCTATCCATAACCATTCCTAATAATTGAAAAAAAAATAATTAATTTAACTAAGAAAAAATAAAACTGAAGATTCATTCTTTTAAACAGACTGAATTGGATTGTACAAAACAGTTTTAAAAATGCCTTATTTTTACAAATTTTCTTCAAAATAGCATTACATCTCCGCTTAATCCAAGAAAGCCAATACGCACTGAAGTGTTGTAACAGTATATATTGATATATATTTGATGTAGTGGCATTATATGATTAATATTAATATAACAATTAATATATACAGAGAATGTATGATGGGATTCCTAAAGCAAAAAAGGTTGGAATCCTTTCAGTTGGTGTTCCAGGAGAAGTTGCAGGACTTTATGAAGCTTGGAGACAATATGGAAACTGTTCATGGGCAGATCTTGTTGAACCAGCTATGCTTCTTGCTCGTAACGGGTTCAAGATATCTCAAAACCTTCATGATCAAATGAGTGCAAATGAATCAGCGATCACTAGTGATATAGGACTTGCGAGCATATTCACAAAAATGAATGGAACAAAAAGAATTTTGTTGAAAAGTGGAGAAACTGCGTACAACAAGAAACTGGCAGAAACTCTAGAAACTATTGCTGAATATGGTGCGGGTGCCTTGTATGATAATAAGTCAGTTGGAATTGATTTGATTAAAGACATTGAAGAACGTAAAGGGATAGTCTCAATGAGGGACTTGAGGAGATATAGAGTTGAAATAAGAAAGCCCATCTTCTCCTCTACCATTGCCGGTCTTGATATCATCACCGCCCCTCCTCCTTCAGGAGGTTCTGGACTCATCCTTGTAAGTTTAATTTTATATATTTTTTAAAAAAATTAATTATTTTTAGGGGTTTCAATGTAGGATATTTTGGACCACATTAACATCTTAAATCACCAATTGATTTAAAAGCTTAAGATATATAATGGGTGAATGACAAATGACGAATTTAATTATATTTCACTTAATTAACACTCTCTCTCACTTGTGGACTTGAAATAGGAAGAAAGACTCAAACAAATAGAAATGAATATTAAATGGGGAGGAACAATAATGTTGGGCTTGAACAGAAGACATCTTGGATCACATTGCTCTGATACCATCTTAAGTAACCAATTAACTCATTGTTTGAACCTTTAGTTGAATTGGTGATTTAAGATAATATTAGAGCAGGTGGTCCAGAGAGATCTTGTGTTCAAACCCTGCAATGTTATAACTTGTCTCCATTTAAAATTGATTTTCACTTGTTAGATTTTCTTCAACGATGTTTACGTTCTTAATCACTCATTAAATATTACCATCTTTAAATTAAATTTTCAAATTAATTAAAAAGGTTGTATCATTGTGCAGCTGCTTAATATTCTAGCACGATATAAAGTACATGATCGAGGGCCTCTAAGTATCCACAGGGAAATTGAAGCATTGAAACATGTAATTGCTGTAAGAACATACTTAGGTGACCCCGATTTCTACAATATCTCACAAGTACTTTCCGACATGCTCTCACCCCAATTTGCTGAAGAATTACATAAAGCCATTAATGACAACACTACGTTTGATTCAAACTATTACGGTGGCAGGTCAGTGTTCCCTTCCCTTTTACTTTTCTAGTCCTAAATTTTTAATTTTTCGATTAATTAATTAATTATATTGATTGTGTTTAATTTAAGTTGTAAAACAGATGGAAACCAAACAATGAGGAGGGCACTACTCATTTGTGCGTAATCGATAAAGAACGAAATGCTGTGTCACTTACAAGCACAGTGAACAACTATTTTGGCTCACATATATTGTCCCCAAGAACTGGAATTGTGTTAAACAATCAAATGGATGATTTTCTATCCCCAATAGTAATACTGCGGATGATTTCCCACCAGCACCATCCAATTTCATTAGGCCAGGGAAAAGGCCATTATCATCTGTATCTCCTACTATTCTCCTTAAGGTCATACTAATTTTTAACTTCATATAACTACATCTTTTAATCACTCAAAATTATCTCTTTTTCCAACATTTCTATGAAGAAATAAAAAACATTTGTACACAGGATGGTGAACTAAAAGCTGTGGTTGGTGCAAGTGGAGGAAGATTCATCATTGGTGCAGTAGCACAGGTTCTGTTGAATCATTTTACAAAAGGAATGGATCCTTTCAAATCTGTTATGACTCCCAGAGTTTATCATACGGTTCGTGCCCTAAAATTCCTAATATTTTCTAACAAATATTTAATATTTTTTACTAACAAATATTTAATTGCAATTAGTCGGTTCTAATAAAGTAATTTAAAATATGATTTTTTTGTGTCCACCAATAATTAGCTTGTATTGAGATATTCAATTGTCAATATTTGATAGTACTGGGTTTGTTTTATTGGACCAAATACTTGTTTGAGTTTTATGGGCTTATGAGGTTCTATCTCAAAACCAGTTGACAATGATATGGATAACAAGTTATCTATCTTATACATAATGAGATAAATGTCTATCGGTGTATATATTAGTAATAGATGATGATAGTCTAACTTGTTTTTTATTATTATTGTTCTTTCTTGTTCTTGTGGTGTGTTTGAACAAACAGTTGTTCCCAAATATATTGACATATGAGAACTGGACAAGAAATGAAGATCGTTTTGAAGTTAGTAATGAAATAGTGAAATTTCTTGCTACGAAGAATCACAAGTTAAAAGGGGTTAGCGGAACGACGACAGTTTGCCAATTCATTGTTCAAGATTTTAACTCACCTGGAAAACTTACAGCGGTGAGTGATCCTAGAAAGGGTGGGGCTCCTTATGGCTATGATCATGAATTATTGTCGGAGGATCTTTGTAACAATAATCCTCCATTGTTAAACACATATTATAGTCAAAATGATCATTTCTCTGATCATGGTCTCCTTTAA

mRNA sequence

ATGAACTATAATAAGGTCATCGTTGTTGTAATTTCTACTGTGTTGTTATTGTTGCCTTTCACCTCCATAGCTCGACCTGAAGTTATTGAAGCAAGTCTTGGCGCTGTTGCTGCAGATGATCATCGATGCTCGAAAATCGGTGTGTATGTTCTACAAAAATTAAAAGGAAATGCTGTTGATGCGGCTGTGGCTACAGCTCTTTGTCAAGGGGTTGTTAGTCCAGCATCAAGTGGCATTGGTGGAGGAGGCTTTATGTTGATCAGATTGGCCAATGGAGAGTCTCAAGTTTTTGATATGAGGGAGATTGCACCTTTGAAAGCTCATGAGAGAATGTATGATGGGATTCCTAAAGCAAAAAAGGTTGGAATCCTTTCAGTTGGTGTTCCAGGAGAAGTTGCAGGACTTTATGAAGCTTGGAGACAATATGGAAACTGTTCATGGGCAGATCTTGTTGAACCAGCTATGCTTCTTGCTCGTAACGGGTTCAAGATATCTCAAAACCTTCATGATCAAATGAGTGCAAATGAATCAGCGATCACTAGTGATATAGGACTTGCGAGCATATTCACAAAAATGAATGGAACAAAAAGAATTTTGTTGAAAAGTGGAGAAACTGCGTACAACAAGAAACTGGCAGAAACTCTAGAAACTATTGCTGAATATGGTGCGGGTGCCTTGTATGATAATAAGTCAGTTGGAATTGATTTGATTAAAGACATTGAAGAACGTAAAGGGATAGTCTCAATGAGGGACTTGAGGAGATATAGAGTTGAAATAAGAAAGCCCATCTTCTCCTCTACCATTGCCGGTCTTGATATCATCACCGCCCCTCCTCCTTCAGGAGGTTCTGGACTCATCCTTCTGCTTAATATTCTAGCACGATATAAAGTACATGATCGAGGGCCTCTAAGTATCCACAGGGAAATTGAAGCATTGAAACATGTAATTGCTGTAAGAACATACTTAGGTGACCCCGATTTCTACAATATCTCACAAGTACTTTCCGACATGCTCTCACCCCAATTTGCTGAAGAATTACATAAAGCCATTAATGACAACACTACGTTTGATTCAAACTATTACGGTGGCAGATGGAAACCAAACAATGAGGAGGGCACTACTCATTTGTGCGTAATCGATAAAGAACGAAATGCTGTGTCACTTACAAGCACAGTGAACAACTATTTTGGCTCACATATATTGTCCCCAAGAACTGGAATTGTTAATACTGCGGATGATTTCCCACCAGCACCATCCAATTTCATTAGGCCAGGGAAAAGGCCATTATCATCTGTATCTCCTACTATTCTCCTTAAGGATGGTGAACTAAAAGCTGTGGTTGGTGCAAGTGGAGGAAGATTCATCATTGGTGCAGTAGCACAGGTTCTGTTGAATCATTTTACAAAAGGAATGGATCCTTTCAAATCTGTTATGACTCCCAGAGTTTATCATACGTTGTTCCCAAATATATTGACATATGAGAACTGGACAAGAAATGAAGATCGTTTTGAAGTTAGTAATGAAATAGTGAAATTTCTTGCTACGAAGAATCACAAGTTAAAAGGGGTTAGCGGAACGACGACAGTTTGCCAATTCATTGTTCAAGATTTTAACTCACCTGGAAAACTTACAGCGGTGAGTGATCCTAGAAAGGGTGGGGCTCCTTATGGCTATGATCATGAATTATTGTCGGAGGATCTTTGTAACAATAATCCTCCATTGTTAAACACATATTATAGTCAAAATGATCATTTCTCTGATCATGGTCTCCTTTAA

Coding sequence (CDS)

ATGAACTATAATAAGGTCATCGTTGTTGTAATTTCTACTGTGTTGTTATTGTTGCCTTTCACCTCCATAGCTCGACCTGAAGTTATTGAAGCAAGTCTTGGCGCTGTTGCTGCAGATGATCATCGATGCTCGAAAATCGGTGTGTATGTTCTACAAAAATTAAAAGGAAATGCTGTTGATGCGGCTGTGGCTACAGCTCTTTGTCAAGGGGTTGTTAGTCCAGCATCAAGTGGCATTGGTGGAGGAGGCTTTATGTTGATCAGATTGGCCAATGGAGAGTCTCAAGTTTTTGATATGAGGGAGATTGCACCTTTGAAAGCTCATGAGAGAATGTATGATGGGATTCCTAAAGCAAAAAAGGTTGGAATCCTTTCAGTTGGTGTTCCAGGAGAAGTTGCAGGACTTTATGAAGCTTGGAGACAATATGGAAACTGTTCATGGGCAGATCTTGTTGAACCAGCTATGCTTCTTGCTCGTAACGGGTTCAAGATATCTCAAAACCTTCATGATCAAATGAGTGCAAATGAATCAGCGATCACTAGTGATATAGGACTTGCGAGCATATTCACAAAAATGAATGGAACAAAAAGAATTTTGTTGAAAAGTGGAGAAACTGCGTACAACAAGAAACTGGCAGAAACTCTAGAAACTATTGCTGAATATGGTGCGGGTGCCTTGTATGATAATAAGTCAGTTGGAATTGATTTGATTAAAGACATTGAAGAACGTAAAGGGATAGTCTCAATGAGGGACTTGAGGAGATATAGAGTTGAAATAAGAAAGCCCATCTTCTCCTCTACCATTGCCGGTCTTGATATCATCACCGCCCCTCCTCCTTCAGGAGGTTCTGGACTCATCCTTCTGCTTAATATTCTAGCACGATATAAAGTACATGATCGAGGGCCTCTAAGTATCCACAGGGAAATTGAAGCATTGAAACATGTAATTGCTGTAAGAACATACTTAGGTGACCCCGATTTCTACAATATCTCACAAGTACTTTCCGACATGCTCTCACCCCAATTTGCTGAAGAATTACATAAAGCCATTAATGACAACACTACGTTTGATTCAAACTATTACGGTGGCAGATGGAAACCAAACAATGAGGAGGGCACTACTCATTTGTGCGTAATCGATAAAGAACGAAATGCTGTGTCACTTACAAGCACAGTGAACAACTATTTTGGCTCACATATATTGTCCCCAAGAACTGGAATTGTTAATACTGCGGATGATTTCCCACCAGCACCATCCAATTTCATTAGGCCAGGGAAAAGGCCATTATCATCTGTATCTCCTACTATTCTCCTTAAGGATGGTGAACTAAAAGCTGTGGTTGGTGCAAGTGGAGGAAGATTCATCATTGGTGCAGTAGCACAGGTTCTGTTGAATCATTTTACAAAAGGAATGGATCCTTTCAAATCTGTTATGACTCCCAGAGTTTATCATACGTTGTTCCCAAATATATTGACATATGAGAACTGGACAAGAAATGAAGATCGTTTTGAAGTTAGTAATGAAATAGTGAAATTTCTTGCTACGAAGAATCACAAGTTAAAAGGGGTTAGCGGAACGACGACAGTTTGCCAATTCATTGTTCAAGATTTTAACTCACCTGGAAAACTTACAGCGGTGAGTGATCCTAGAAAGGGTGGGGCTCCTTATGGCTATGATCATGAATTATTGTCGGAGGATCTTTGTAACAATAATCCTCCATTGTTAAACACATATTATAGTCAAAATGATCATTTCTCTGATCATGGTCTCCTTTAA

Protein sequence

MNYNKVIVVVISTVLLLLPFTSIARPEVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVDAAVATALCQGVVSPASSGIGGGGFMLIRLANGESQVFDMREIAPLKAHERMYDGIPKAKKVGILSVGVPGEVAGLYEAWRQYGNCSWADLVEPAMLLARNGFKISQNLHDQMSANESAITSDIGLASIFTKMNGTKRILLKSGETAYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDIEERKGIVSMRDLRRYRVEIRKPIFSSTIAGLDIITAPPPSGGSGLILLLNILARYKVHDRGPLSIHREIEALKHVIAVRTYLGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFDSNYYGGRWKPNNEEGTTHLCVIDKERNAVSLTSTVNNYFGSHILSPRTGIVNTADDFPPAPSNFIRPGKRPLSSVSPTILLKDGELKAVVGASGGRFIIGAVAQVLLNHFTKGMDPFKSVMTPRVYHTLFPNILTYENWTRNEDRFEVSNEIVKFLATKNHKLKGVSGTTTVCQFIVQDFNSPGKLTAVSDPRKGGAPYGYDHELLSEDLCNNNPPLLNTYYSQNDHFSDHGLL
Homology
BLAST of PI0005633 vs. ExPASy Swiss-Prot
Match: Q8VYW6 (Glutathione hydrolase 1 OS=Arabidopsis thaliana OX=3702 GN=GGT1 PE=2 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 3.1e-150
Identity = 301/581 (51.81%), Postives = 390/581 (67.13%), Query Frame = 0

Query: 1   MNYNKVIVVVISTVLLLLPFTSIARPEVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVD 60
           M+  + + +V+  +  L    +  R + I  S GAVA DD RCS IG+ VL++  GNA+D
Sbjct: 1   MSLVRTVTIVLFIIAFLQNAAAQKRQQSIVKSRGAVATDDGRCSVIGMRVLRE-GGNAID 60

Query: 61  AAVATALCQGVVSPASSGIGGGGFMLIRLANGESQVFDMREIAPLKAHERMYDGIPKAKK 120
           A+VA ALC GVVSPASSGIGGG F ++++A G+   +D RE APL+A E MY G    KK
Sbjct: 61  ASVAAALCLGVVSPASSGIGGGAFTVVKIAGGKEIAYDSRETAPLRATENMYGGNVDLKK 120

Query: 121 VGILSVGVPGEVAGLYEAWRQYGNCSWADLVEPAMLLARNGFKISQNLHDQMSANESAIT 180
            G LSVGVPGEVAGL+ AW+Q+G   W  LV PA  LA  GFKIS+ L+ QM+A  S I 
Sbjct: 121 KGALSVGVPGEVAGLFTAWKQHGKLPWKRLVTPAEKLA-EGFKISKYLYMQMNATRSDIL 180

Query: 181 SDIGLASIFTKMNGTKRILLKSGETAYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDI 240
           +D GL+ +F   NG    L K G   +N KLA TL+ I EYG  A Y N +VG++L +DI
Sbjct: 181 ADKGLSDLFVS-NGE---LKKPGTICHNPKLALTLKLIGEYGPKAFY-NGTVGVNLARDI 240

Query: 241 EERKGIVSMRDLRRYRVEIRKPIFSSTIAGLDIITAPPP-SGGSGLILLLNILARYKVHD 300
           ++  GI++++DL+ YRV+I++P+ S+ I G  ++  PPP SGG+ ++L+LNIL++Y +  
Sbjct: 241 KKSGGIITLKDLQSYRVKIKEPL-SADILGYRVLGMPPPSSGGAAMMLVLNILSQYGIPS 300

Query: 301 --RGPLSIHREIEALKHVIAVRTYLGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFD 360
              GPL +HR IEALKH  AVR  LGDPDF ++++V+SDMLSP+FA++L   IND  TFD
Sbjct: 301 GVSGPLGVHRLIEALKHAFAVRMNLGDPDFTDVTKVVSDMLSPKFAKDLKSKINDQKTFD 360

Query: 361 SNYYGGRWKPNNEEGTTHLCVIDKERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF- 420
             YYGG W   ++ GT+HL +ID+ERNAVS+TST+N YFG+ +LSP TGIV  N  DDF 
Sbjct: 361 PKYYGGMWNQIDDHGTSHLSIIDRERNAVSMTSTINGYFGALMLSPSTGIVLNNEMDDFS 420

Query: 421 ------------PPAPSNFIRPGKRPLSSVSPTILLKDGELKAVVGASGGRFIIGAVAQV 480
                       PPAP+NFIRPGKRPLSS+SPTI+LKDG++KA VGASGG  II    +V
Sbjct: 421 IPMKSNGNLDVPPPAPANFIRPGKRPLSSMSPTIVLKDGKVKAAVGASGGANIIAGTTEV 480

Query: 481 LLNHFTKGMDPFKSVMTPRVYHTLFPNILTYENWTR-NEDRFEVSNEIVKFLATKNHKLK 540
            LNHF   MDP  SV+ PR+YH L PN  +YENWT    D FE+       L  K H L 
Sbjct: 481 YLNHFFLKMDPLSSVLAPRIYHQLIPNRASYENWTTVFNDHFEIPKATRVVLEKKGHVLS 540

Query: 541 GVSGTTTVCQFIVQDF--NSPGK--LTAVSDPRKGGAPYGY 559
            ++G  T+ QFIVQ+   NS G+  L AVSDPRKGG P GY
Sbjct: 541 PIAG-GTIAQFIVQESGENSGGRSELVAVSDPRKGGFPSGY 572

BLAST of PI0005633 vs. ExPASy Swiss-Prot
Match: Q680I5 (Glutathione hydrolase 2 OS=Arabidopsis thaliana OX=3702 GN=GGT2 PE=2 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 3.1e-150
Identity = 306/586 (52.22%), Postives = 384/586 (65.53%), Query Frame = 0

Query: 1   MNYNKVIVVVISTVLLLLPF----TSIARPEVIEASLGAVAADDHRCSKIGVYVLQKLKG 60
           MN    +V   +  LLL+ F     ++   + I A  GAVA DD RCS IG  VL++  G
Sbjct: 1   MNSFMSLVRTATIALLLIAFLQNANAVKNLQSIVAYHGAVATDDGRCSAIGTNVLRQ-GG 60

Query: 61  NAVDAAVATALCQGVVSPASSGIGGGGFMLIRLANGESQVFDMREIAPLKAHERMYDGIP 120
           NA+DA+VA ALC GVVSPASSGIGGG F +I+LANG    +D RE APL A E MY   P
Sbjct: 61  NAIDASVAAALCLGVVSPASSGIGGGAFTMIKLANGTEVAYDSRETAPLSATEDMYGDNP 120

Query: 121 KAKKVGILSVGVPGEVAGLYEAWRQYGNCSWADLVEPAMLLARNGFKISQNLHDQMSANE 180
           + KK G LSVGVPGEVAGLY AW Q+G   W  LVEPA  LA  GFKIS+ L+ QM+A  
Sbjct: 121 ERKKKGSLSVGVPGEVAGLYTAWTQHGKLPWKQLVEPAEKLAAEGFKISKYLYMQMNATR 180

Query: 181 SAITSDIGLASIFTKMNGTKRILLKSGETAYNKKLAETLETIAEYGAGALYDNKSVGIDL 240
           S I +D GL+ +F   NG    L K G    N KLA+TL  IAEYG  A Y N +VG +L
Sbjct: 181 SDILADKGLSELFVS-NGE---LKKPGAICRNPKLADTLSQIAEYGPKAFY-NGTVGFNL 240

Query: 241 IKDIEERKGIVSMRDLRRYRVEIRKPIFSSTIAGLDIITAPPP-SGGSGLILLLNILARY 300
           + DI++  GI++++DL+ Y V++++P+ S+ I G  ++  PPP SGG  ++L+LNILA+Y
Sbjct: 241 VSDIQKAGGIITLKDLQNYNVKVKEPL-STEILGYRLLGMPPPSSGGPAMMLILNILAQY 300

Query: 301 KVHD--RGPLSIHREIEALKHVIAVRTYLGDPDFY-NISQVLSDMLSPQFAEELHKAIND 360
            +     GPL +HR +EALKH  AVR  LGDPDF   ++ V++DMLSP+FA++L   IND
Sbjct: 301 GIPSGVSGPLGVHRLVEALKHAFAVRMNLGDPDFVPEVTNVVADMLSPKFAQDLKSKIND 360

Query: 361 NTTFDSNYYGGRWKPNNEEGTTHLCVIDKERNAVSLTSTVNNYFGSHILSPRTGIV--NT 420
             TFD  YYGG+W    + GT+HL +ID ERNAVS+TST+N YFG+ +LSP TGIV  N 
Sbjct: 361 EKTFDPKYYGGKWGQIKDHGTSHLSIIDSERNAVSMTSTINGYFGAIMLSPSTGIVLNNE 420

Query: 421 ADDF-------------PPAPSNFIRPGKRPLSSVSPTILLKDGELKAVVGASGGRFIIG 480
            DDF             PPAP+NFIRPGKRPLSS++PTI+LKDG++KA +GASGG +II 
Sbjct: 421 MDDFSIPTKSGGDPDVPPPAPANFIRPGKRPLSSMTPTIVLKDGKVKAALGASGGMYIIA 480

Query: 481 AVAQVLLNHFTKGMDPFKSVMTPRVYHTLFPNILTYENWTR-NEDRFEVSNEIVKFLATK 540
              QV LNHF   MDP  SV+ PR+YH L PN  +YENWT    D FE+  EI   L  K
Sbjct: 481 GTTQVYLNHFFLNMDPLSSVVAPRIYHQLIPNKASYENWTTVYSDHFEIPEEIRLVLEKK 540

Query: 541 NHKLKGVSGTTTVCQFIVQ--DFNSPG--KLTAVSDPRKGGAPYGY 559
              L  ++G  T+ Q IV+  D  S G  KL AVSDPRKGG P GY
Sbjct: 541 GQVLTPIAG-GTISQLIVEQSDGKSGGISKLVAVSDPRKGGFPSGY 578

BLAST of PI0005633 vs. ExPASy Swiss-Prot
Match: Q9M0G0 (Glutathione hydrolase 3 OS=Arabidopsis thaliana OX=3702 GN=GGT3 PE=2 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 1.6e-130
Identity = 267/569 (46.92%), Postives = 355/569 (62.39%), Query Frame = 0

Query: 27  EVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVDAAVATALCQGVVSPASSGIGGGGFML 86
           +V+E+  G VAADD RCS+IG  VL+   G+AVDAAVA  LC GVV+P SSGIGGG F++
Sbjct: 74  DVVESENGVVAADDARCSEIGASVLRS-GGHAVDAAVAITLCVGVVNPMSSGIGGGSFLI 133

Query: 87  I-RLANGESQVFDMREIAPLKAHERMYDGIPKAKKVGILSVGVPGEVAGLYEAWRQYGNC 146
           +    + +++ FDMRE APL A + MY     AK +G LS+GVPGE+AGLYEAW++YG  
Sbjct: 134 VSSQKDSKAEAFDMRETAPLAASKDMYKNDASAKSLGALSMGVPGEIAGLYEAWKRYGRL 193

Query: 147 SWADLVEPAMLLARNGFKISQNLHDQMSANESAITSDIGLASIFTKMNGTKRILLKSGET 206
            W  L EPA+ LAR+GF +   L   +S   + I  D G+ S+F++ NG    +LK+GET
Sbjct: 194 PWKPLFEPAIKLARDGFVVYPYLGKAISTKVAMILKDPGMRSVFSR-NGQ---VLKTGET 253

Query: 207 AYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDIEERKGIVSMRDLRRYRVEIRKPIFS 266
            YN +LA++LETI+E G GA Y N +VG  L+KD+++  GI++M DLR Y+V +   + S
Sbjct: 254 CYNPELAQSLETISEQGPGAFY-NGTVGEKLVKDVKKAGGIITMDDLRSYKVRVTDAM-S 313

Query: 267 STIAGLDIITAPPPSGGS-GLILLLNILARYK---VHDRGPLSIHREIEALKHVIAVRTY 326
             + G  +   PPPSGG+ G  +++NIL  Y          L +HR IEA+KH+ A R  
Sbjct: 314 VDVMGYTVHGMPPPSGGTVGFSMVMNILDSYSNLYTASGRELGLHRLIEAMKHMFAARMD 373

Query: 327 LGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFDSNYYGGRWKPNNEEGTTHLCVIDK 386
           LGDP+F N++  ++ MLS   AEE+ K I DNTTF   YY  RW    ++GT+H CV+D 
Sbjct: 374 LGDPEFVNVTNSMNQMLSKAHAEEIQKRIFDNTTFPPEYYMNRWSQLRDQGTSHFCVVDA 433

Query: 387 ERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF-----------PPAPSNFIRPGKRP 446
           +RN+VS+TSTVN  FG+ +LSP TGIV  N  DDF           PPAP+NFI P KRP
Sbjct: 434 DRNSVSMTSTVNYRFGAGVLSPSTGIVLNNEMDDFSTPTEITPDMLPPAPTNFIEPNKRP 493

Query: 447 LSSVSPTILLKDGELKAVVGASGGRFIIGAVAQVLLNHFTKGMDPFKSVMTPRVYHTLFP 506
           LSS++P ++ KDGE  A +G +GG  II AV QV LN F   M P ++V + R+YH L P
Sbjct: 494 LSSMTPLVITKDGEFVAALGGAGGMHIIPAVLQVFLNCFVLNMKPKEAVESARIYHRLIP 553

Query: 507 NILTYENWTR-NEDRFEVSNEIVKFLATKNHKLKGVSGTTTVCQFIVQDFNS-------- 556
           N+++YEN+T  N D   VS +   FLA + H+LK +SG   V Q IVQ F          
Sbjct: 554 NVVSYENFTTINGDHIGVSEDTKMFLAERGHELKELSGGAIV-QLIVQSFKEEKEEEMII 613

BLAST of PI0005633 vs. ExPASy Swiss-Prot
Match: Q9US04 (Glutathione hydrolase proenzyme 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ggt1 PE=2 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 4.3e-88
Identity = 201/552 (36.41%), Postives = 298/552 (53.99%), Query Frame = 0

Query: 28  VIEASLGAVAADDHRCSKIGVYVLQKLKGNAVDAAVATALCQGVVSPASSGIGGGGFMLI 87
           V+E   G VA ++  CS+IGV +L K  GNAVDAA+A+ +C G V+  SSGIGGGGFMLI
Sbjct: 94  VVEGKNGVVATEEETCSQIGVGIL-KAGGNAVDAAIASGICIGAVNSFSSGIGGGGFMLI 153

Query: 88  RLANGESQVFDMREIAPLKAHERMYDGIPKAKKVGILSVGVPGEVAGLYEAWRQYGNCSW 147
           R  NG +   + RE AP  A + M+ G     +VG LSV VPGE+AG   AW+ YG+  W
Sbjct: 154 RHPNGTAHSLNFRETAPAGASKNMFHGNSTLSQVGGLSVAVPGEIAGYERAWKMYGSLPW 213

Query: 148 ADLVEPAMLLARNGFKISQNLHDQMSANESAITSDIGLASIFTKMNGTKRILLKSGETAY 207
             L EP + L R+G  + + L  ++   E    S       ++K+   + + L  GE  Y
Sbjct: 214 HKLFEPTIRLMRDGMPMPKELASRIRRPE---FSYFKTHPDWSKIFAPEGVFLHVGEKFY 273

Query: 208 NKKLAETLETIAEYGAGALYDNKSVGIDLIKDIEERKGIVSMRDLRRYRVEIRKPIFSST 267
              LA TLE IA++G    Y  K +   L+K ++++ GI++M D+  + V + +PI+ + 
Sbjct: 274 RPALASTLEEIAKFGPEVFYTGK-IAERLVKFVQQQGGILTMEDMANFSVVVEEPIYGN- 333

Query: 268 IAGLDIITAPPPSGGSGLILLLNILARYKVH--------DRGPLSIHREIEALKHVIAVR 327
               ++IT   P  G  LIL LN+L++  +         +   + +H  IE +K + A R
Sbjct: 334 FYDREVITCGSPCSGEALILGLNVLSKVDLSEGTSILGCEMTDIGVHHLIETMKWMSAGR 393

Query: 328 TYLGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFDSNYYGGRWKPNNEEGTTHLCVI 387
           T L DP FYN +  +  +LS ++A+E+   I++  TFD  +Y   +   N+ GTTHL VI
Sbjct: 394 TVLADPTFYNNTDHVEQLLSLEYADEIRNNISNERTFDFTHYKAEYDFPNDHGTTHLSVI 453

Query: 388 DKERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF-----------PPAPSNFIRPGK 447
           DK+  AV LT+++N  FGS +L P TGI+  +  DDF            P+P NFI PGK
Sbjct: 454 DKDNMAVGLTASINLMFGSQLLEPETGIILNDHMDDFASPGIVNAFGLSPSPYNFIAPGK 513

Query: 448 RPLSSVSPTILLKDGELKAVVGASGGRFIIGAVAQVLLNHFTKGMDPFKSVMTPRVYHTL 507
           RP SS  PTIL+ +GE++ V+G SGG  I+ AV   ++  +  G    +SV +PR +H L
Sbjct: 514 RPQSSAVPTILVYNGEVEMVLGGSGGSRIVTAVLDTIIKKYKWGKSLLESVESPRFHHQL 573

Query: 508 FPNILTYENWTRNEDRFEVSNEIVKFLATKNHKLKGVSGTTTVCQFIVQDFNSPGKLTAV 559
            PNI+  +          V  E+++ L    H +  +       + I   F + G L  +
Sbjct: 574 MPNIVYIDE--------TVEIEVLRALEKFGHIVDLIPVQYPFSE-IQAVFRTNGTLYGL 630

BLAST of PI0005633 vs. ExPASy Swiss-Prot
Match: Q60928 (Glutathione hydrolase 1 proenzyme OS=Mus musculus OX=10090 GN=Ggt1 PE=1 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 1.4e-86
Identity = 211/575 (36.70%), Postives = 318/575 (55.30%), Query Frame = 0

Query: 6   VIVVVISTVLLLLPFTSIARPEVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVDAAVAT 65
           V+V VI  + + LP+TS  +P+ +  S  AVA D  RCS+IG  +LQ+  G+ VDAA+A+
Sbjct: 14  VLVFVIIGLCIWLPYTS-GKPDHV-YSRAAVATDAKRCSEIGRDILQE-GGSVVDAAIAS 73

Query: 66  ALCQGVVSPASSGIGGGGFMLI-RLANGESQVFDMREIAPLKAHERMYDGIPKAKKVGIL 125
            LC G+++  S GIGGG F  I     G+ +V + RE+AP  A+  M++   K  + G L
Sbjct: 74  LLCMGLMNAHSMGIGGGLFFTIYNSTTGKVEVINAREVAPRLANTTMFNN-SKDSEEGGL 133

Query: 126 SVGVPGEVAGLYEAWRQYGNCSWADLVEPAMLLARNGFKISQNLHDQMSANESAITSDIG 185
           SV VPGE+ G   A +++G   WA L +P++ LAR+GF + + L   +      I     
Sbjct: 134 SVAVPGEIRGYELAHQRHGRLPWARLFQPSIQLARHGFPVGKGLAIALDKKRDVIEKTPA 193

Query: 186 LASIFTKMNGTKRILLKSGETAYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDIEERK 245
           L  +F +       +L+ GET    KLA+TL+ +A+ GA A Y N S+   ++KDI+E  
Sbjct: 194 LCEVFCRQGK----VLQEGETVTMPKLADTLQILAQEGAKAFY-NGSLTAQIVKDIQEAG 253

Query: 246 GIVSMRDLRRYRVEIRKPIFSSTIAGLDIITAPPPSGGSGLILLLNILARYK-------V 305
           GI+++ DL  YR E+ +   S  +    +     P  G  LIL+LNIL  Y         
Sbjct: 254 GIMTVEDLNNYRAELIEHPMSIGLGDATLYVPSAPLSGPVLILILNILKGYNFSPKSVAT 313

Query: 306 HDRGPLSIHREIEALKHVIAVRTYLGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFD 365
            ++  L+ HR +EA +   A RT LGDP F ++SQV+ +M S  +A +L   I D TT  
Sbjct: 314 PEQKALTYHRIVEAFRFAYAKRTMLGDPKFVDVSQVIRNMSSEFYATQLRARITDETTHP 373

Query: 366 SNYYGGRWKPNNEEGTTHLCVIDKERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF- 425
           + YY   +   ++ GT HL  + ++ +AV+ TST+N YFGS +LS  +GI+  +  DDF 
Sbjct: 374 AAYYEPEFYLQDDGGTAHLSAVSEDGSAVAATSTINLYFGSKVLSRVSGILFNDEMDDFS 433

Query: 426 ----------PPAPSNFIRPGKRPLSSVSPTILL-KDGELKAVVGASGGRFIIGAVAQVL 485
                      P+P+NFI+PGK+PLSS+ P+I+L KDG+++ VVGASGG  I  +VA  +
Sbjct: 434 SPNFINQFRVAPSPANFIKPGKQPLSSMCPSIILDKDGQVRMVVGASGGTQITTSVALAI 493

Query: 486 LNHFTKGMDPFKSVMTPRVYHTLFPNILTYENWTRNEDRFEVSNEIVKFLATKNHKLKGV 545
           +N    G D  ++V  PR+++ L PN  T E         ++   +   L  ++H  +  
Sbjct: 494 INSLWFGYDVKRAVEEPRLHNQLLPNTTTVEK--------DIDQVVTAGLKIRHHHTEVT 553

Query: 546 SGTTTVCQFIVQDFNSPGKLTAVSDPRKGGAPYGY 559
                V Q +V+   + G   A SD RKGG P GY
Sbjct: 554 PTFIAVVQAVVR---ASGGWAAASDSRKGGEPAGY 568

BLAST of PI0005633 vs. ExPASy TrEMBL
Match: A0A6J1HG57 (glutathione hydrolase 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111463200 PE=4 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 5.7e-184
Identity = 360/574 (62.72%), Postives = 430/574 (74.91%), Query Frame = 0

Query: 7   IVVVISTVLLLLPFTSIARPEVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVDAAVATA 66
           +++V+ ++ LL P TSIARPEVI AS GAVAADD RCS+IG+ VL++  G+AVDA+VATA
Sbjct: 9   LIIVLVSIALLTP-TSIARPEVIVASHGAVAADDSRCSRIGMDVLRE-GGHAVDASVATA 68

Query: 67  LCQGVVSPASSGIGGGGFMLIRLANGESQVFDMREIAPLKAHERMYDGIPKAKKVGILSV 126
           LC GVVS ASSGIGGG FMLIR A+G+SQ FDMRE APL AHE MY     +K VG LS+
Sbjct: 69  LCLGVVSSASSGIGGGAFMLIRSASGKSQAFDMRETAPLLAHENMYASNAASKAVGALSI 128

Query: 127 GVPGEVAGLYEAWRQYGNCSWADLVEPAMLLARNGFKISQNLHDQMSANESAITSDIGLA 186
            VPGE+AGL+EAW+QYG   WA LV+PA  LAR GFKIS  LH QM   E+ I  D GL 
Sbjct: 129 AVPGELAGLHEAWKQYGKLPWARLVQPAERLARLGFKISPYLHMQMVKTEAGILGDEGLR 188

Query: 187 SIFTKMNGTKRILLKSGETAYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDIEERKGI 246
           ++FT  +G K  LLK G+   NKKLA+TL TI+ +G  A Y N S+G++LI+DI +  GI
Sbjct: 189 NLFT--SGGK--LLKPGDVIRNKKLAQTLRTISRFGVQAFY-NGSIGVNLIRDIRKLGGI 248

Query: 247 VSMRDLRRYRVEIRKPIFSSTIAGLDIITAPPPSGGSGLILLLNILARYKVHD--RGPLS 306
           +SM+DL+ YRV+IR PI + T+ G +IIT PPPSGGSGLIL+LNILA+YK+     GPL 
Sbjct: 249 LSMKDLQSYRVKIRVPISARTM-GHEIITMPPPSGGSGLILMLNILAQYKLPSGLMGPLG 308

Query: 307 IHREIEALKHVIAVRTYLGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFDSNYYGGR 366
           IHREIEALKHV AVRT LGDP+FYN+S+VLSDMLSP+FA +L K INDN TFD ++YGGR
Sbjct: 309 IHREIEALKHVAAVRTNLGDPNFYNVSKVLSDMLSPKFARKLKKTINDNRTFDPSHYGGR 368

Query: 367 WKPNNEEGTTHLCVIDKERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF-------- 426
           W P  + GT+HL V+D ERNAVS+T+TVN YFG+ ILSP TGI+  N  DDF        
Sbjct: 369 WNPIYDHGTSHLSVVDGERNAVSMTTTVNYYFGAVILSPSTGIILNNEMDDFSVPANVSA 428

Query: 427 ---PPAPSNFIRPGKRPLSSVSPTILLKDGELKAVVGASGGRFIIGAVAQVLLNHFTKGM 486
              PPAPSNFIRPGKRPLSS+SP I+LK+G+LK VVGASGG FII   A+V+LNHF KGM
Sbjct: 429 NTLPPAPSNFIRPGKRPLSSMSPAIVLKNGQLKGVVGASGGGFIIAGTAEVVLNHFAKGM 488

Query: 487 DPFKSVMTPRVYHTLFPNILTYENWTR-NEDRFEVSNEIVKFLATKNHKLKGVSGTTTVC 546
           DPF SVM PRVYH L PN+LTYENWT    D FEV  +    LA K H L+G++G  T+C
Sbjct: 489 DPFSSVMAPRVYHRLIPNVLTYENWTTVLGDHFEVPAQTRAALAKKGHVLEGLAG-GTIC 548

Query: 547 QFIVQDFNSP------GKLTAVSDPRKGGAPYGY 559
           QFIVQ F SP      GKL AVSDPRKGGAP G+
Sbjct: 549 QFIVQKFGSPRQGGGVGKLIAVSDPRKGGAPAGF 573

BLAST of PI0005633 vs. ExPASy TrEMBL
Match: A0A6J1HI63 (glutathione hydrolase 1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111463200 PE=4 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 5.7e-184
Identity = 360/574 (62.72%), Postives = 430/574 (74.91%), Query Frame = 0

Query: 7   IVVVISTVLLLLPFTSIARPEVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVDAAVATA 66
           +++V+ ++ LL P TSIARPEVI AS GAVAADD RCS+IG+ VL++  G+AVDA+VATA
Sbjct: 6   LIIVLVSIALLTP-TSIARPEVIVASHGAVAADDSRCSRIGMDVLRE-GGHAVDASVATA 65

Query: 67  LCQGVVSPASSGIGGGGFMLIRLANGESQVFDMREIAPLKAHERMYDGIPKAKKVGILSV 126
           LC GVVS ASSGIGGG FMLIR A+G+SQ FDMRE APL AHE MY     +K VG LS+
Sbjct: 66  LCLGVVSSASSGIGGGAFMLIRSASGKSQAFDMRETAPLLAHENMYASNAASKAVGALSI 125

Query: 127 GVPGEVAGLYEAWRQYGNCSWADLVEPAMLLARNGFKISQNLHDQMSANESAITSDIGLA 186
            VPGE+AGL+EAW+QYG   WA LV+PA  LAR GFKIS  LH QM   E+ I  D GL 
Sbjct: 126 AVPGELAGLHEAWKQYGKLPWARLVQPAERLARLGFKISPYLHMQMVKTEAGILGDEGLR 185

Query: 187 SIFTKMNGTKRILLKSGETAYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDIEERKGI 246
           ++FT  +G K  LLK G+   NKKLA+TL TI+ +G  A Y N S+G++LI+DI +  GI
Sbjct: 186 NLFT--SGGK--LLKPGDVIRNKKLAQTLRTISRFGVQAFY-NGSIGVNLIRDIRKLGGI 245

Query: 247 VSMRDLRRYRVEIRKPIFSSTIAGLDIITAPPPSGGSGLILLLNILARYKVHD--RGPLS 306
           +SM+DL+ YRV+IR PI + T+ G +IIT PPPSGGSGLIL+LNILA+YK+     GPL 
Sbjct: 246 LSMKDLQSYRVKIRVPISARTM-GHEIITMPPPSGGSGLILMLNILAQYKLPSGLMGPLG 305

Query: 307 IHREIEALKHVIAVRTYLGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFDSNYYGGR 366
           IHREIEALKHV AVRT LGDP+FYN+S+VLSDMLSP+FA +L K INDN TFD ++YGGR
Sbjct: 306 IHREIEALKHVAAVRTNLGDPNFYNVSKVLSDMLSPKFARKLKKTINDNRTFDPSHYGGR 365

Query: 367 WKPNNEEGTTHLCVIDKERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF-------- 426
           W P  + GT+HL V+D ERNAVS+T+TVN YFG+ ILSP TGI+  N  DDF        
Sbjct: 366 WNPIYDHGTSHLSVVDGERNAVSMTTTVNYYFGAVILSPSTGIILNNEMDDFSVPANVSA 425

Query: 427 ---PPAPSNFIRPGKRPLSSVSPTILLKDGELKAVVGASGGRFIIGAVAQVLLNHFTKGM 486
              PPAPSNFIRPGKRPLSS+SP I+LK+G+LK VVGASGG FII   A+V+LNHF KGM
Sbjct: 426 NTLPPAPSNFIRPGKRPLSSMSPAIVLKNGQLKGVVGASGGGFIIAGTAEVVLNHFAKGM 485

Query: 487 DPFKSVMTPRVYHTLFPNILTYENWTR-NEDRFEVSNEIVKFLATKNHKLKGVSGTTTVC 546
           DPF SVM PRVYH L PN+LTYENWT    D FEV  +    LA K H L+G++G  T+C
Sbjct: 486 DPFSSVMAPRVYHRLIPNVLTYENWTTVLGDHFEVPAQTRAALAKKGHVLEGLAG-GTIC 545

Query: 547 QFIVQDFNSP------GKLTAVSDPRKGGAPYGY 559
           QFIVQ F SP      GKL AVSDPRKGGAP G+
Sbjct: 546 QFIVQKFGSPRQGGGVGKLIAVSDPRKGGAPAGF 570

BLAST of PI0005633 vs. ExPASy TrEMBL
Match: A0A6J1KR70 (glutathione hydrolase 1 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111496901 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 3.7e-183
Identity = 357/574 (62.20%), Postives = 431/574 (75.09%), Query Frame = 0

Query: 7   IVVVISTVLLLLPFTSIARPEVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVDAAVATA 66
           +++V+ ++ LL+P TSIARPEVI AS GAVAADD RCS+IG+ VL++  G+AVDA++A A
Sbjct: 6   LIIVLVSIALLIP-TSIARPEVIVASHGAVAADDSRCSRIGMDVLRE-GGHAVDASIAAA 65

Query: 67  LCQGVVSPASSGIGGGGFMLIRLANGESQVFDMREIAPLKAHERMYDGIPKAKKVGILSV 126
           LC GVVS ASSGIGGG FMLIR A+G+SQ FDMRE APL AHE +Y     +K  G LS+
Sbjct: 66  LCLGVVSSASSGIGGGAFMLIRSASGKSQAFDMRETAPLLAHENIYASNVASKAAGALSI 125

Query: 127 GVPGEVAGLYEAWRQYGNCSWADLVEPAMLLARNGFKISQNLHDQMSANESAITSDIGLA 186
            VPGE+AGL+EAW+QYG   WA LV+PA  LAR GFKIS  LH QM   E+ I  D GL 
Sbjct: 126 AVPGELAGLHEAWKQYGKLPWARLVQPAERLARLGFKISPYLHMQMVKTEAGILGDEGLR 185

Query: 187 SIFTKMNGTKRILLKSGETAYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDIEERKGI 246
           ++FT  +G K  LLK G+   NKKLA+TL TI+ +G  A Y N S+GI+LI+DI +  GI
Sbjct: 186 NLFT--SGGK--LLKPGDVIRNKKLAQTLRTISRFGVQAFY-NGSIGINLIRDIRKLGGI 245

Query: 247 VSMRDLRRYRVEIRKPIFSSTIAGLDIITAPPPSGGSGLILLLNILARYKVHD--RGPLS 306
           +SM+DL+ YRV+IR PI + T+ G +IIT PPPSGGSGLIL+LNILA+YK+     GPL 
Sbjct: 246 LSMKDLQSYRVKIRVPISARTM-GHEIITMPPPSGGSGLILMLNILAQYKLPSGLLGPLG 305

Query: 307 IHREIEALKHVIAVRTYLGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFDSNYYGGR 366
           IHREIEALKHV AVRT LGDP+FYN+S+VLSDMLSP+FA +L K INDN TFD ++YGGR
Sbjct: 306 IHREIEALKHVAAVRTNLGDPNFYNVSKVLSDMLSPKFARKLKKTINDNRTFDPSHYGGR 365

Query: 367 WKPNNEEGTTHLCVIDKERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF-------- 426
           W P ++ GT+HL V+D ERNAVS+T+TVN YFG+ ILSP TGI+  N  DDF        
Sbjct: 366 WNPIHDHGTSHLSVVDGERNAVSMTTTVNYYFGAVILSPSTGIILNNEMDDFSVPANVSA 425

Query: 427 ---PPAPSNFIRPGKRPLSSVSPTILLKDGELKAVVGASGGRFIIGAVAQVLLNHFTKGM 486
              PPAPSNFIRPGKRPLSS+SP I+LK+G+LK VVGASGG FII   A+V+LNHF KGM
Sbjct: 426 NKLPPAPSNFIRPGKRPLSSMSPAIVLKNGQLKGVVGASGGGFIIAGTAEVVLNHFAKGM 485

Query: 487 DPFKSVMTPRVYHTLFPNILTYENWTR-NEDRFEVSNEIVKFLATKNHKLKGVSGTTTVC 546
           DPF SVM PRVYH L PN+LTYENWT    D FEV  +    LA K H L+G++G  T+C
Sbjct: 486 DPFSSVMAPRVYHRLIPNVLTYENWTTVLGDHFEVPAQTRAALAKKGHVLEGLAG-GTIC 545

Query: 547 QFIVQDFNSP------GKLTAVSDPRKGGAPYGY 559
           QFIVQ+F SP      GKL AVSDPRKGGAP G+
Sbjct: 546 QFIVQNFGSPRQGGGVGKLIAVSDPRKGGAPAGF 570

BLAST of PI0005633 vs. ExPASy TrEMBL
Match: A0A6J1KLV6 (glutathione hydrolase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496901 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 3.7e-183
Identity = 357/574 (62.20%), Postives = 431/574 (75.09%), Query Frame = 0

Query: 7   IVVVISTVLLLLPFTSIARPEVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVDAAVATA 66
           +++V+ ++ LL+P TSIARPEVI AS GAVAADD RCS+IG+ VL++  G+AVDA++A A
Sbjct: 9   LIIVLVSIALLIP-TSIARPEVIVASHGAVAADDSRCSRIGMDVLRE-GGHAVDASIAAA 68

Query: 67  LCQGVVSPASSGIGGGGFMLIRLANGESQVFDMREIAPLKAHERMYDGIPKAKKVGILSV 126
           LC GVVS ASSGIGGG FMLIR A+G+SQ FDMRE APL AHE +Y     +K  G LS+
Sbjct: 69  LCLGVVSSASSGIGGGAFMLIRSASGKSQAFDMRETAPLLAHENIYASNVASKAAGALSI 128

Query: 127 GVPGEVAGLYEAWRQYGNCSWADLVEPAMLLARNGFKISQNLHDQMSANESAITSDIGLA 186
            VPGE+AGL+EAW+QYG   WA LV+PA  LAR GFKIS  LH QM   E+ I  D GL 
Sbjct: 129 AVPGELAGLHEAWKQYGKLPWARLVQPAERLARLGFKISPYLHMQMVKTEAGILGDEGLR 188

Query: 187 SIFTKMNGTKRILLKSGETAYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDIEERKGI 246
           ++FT  +G K  LLK G+   NKKLA+TL TI+ +G  A Y N S+GI+LI+DI +  GI
Sbjct: 189 NLFT--SGGK--LLKPGDVIRNKKLAQTLRTISRFGVQAFY-NGSIGINLIRDIRKLGGI 248

Query: 247 VSMRDLRRYRVEIRKPIFSSTIAGLDIITAPPPSGGSGLILLLNILARYKVHD--RGPLS 306
           +SM+DL+ YRV+IR PI + T+ G +IIT PPPSGGSGLIL+LNILA+YK+     GPL 
Sbjct: 249 LSMKDLQSYRVKIRVPISARTM-GHEIITMPPPSGGSGLILMLNILAQYKLPSGLLGPLG 308

Query: 307 IHREIEALKHVIAVRTYLGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFDSNYYGGR 366
           IHREIEALKHV AVRT LGDP+FYN+S+VLSDMLSP+FA +L K INDN TFD ++YGGR
Sbjct: 309 IHREIEALKHVAAVRTNLGDPNFYNVSKVLSDMLSPKFARKLKKTINDNRTFDPSHYGGR 368

Query: 367 WKPNNEEGTTHLCVIDKERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF-------- 426
           W P ++ GT+HL V+D ERNAVS+T+TVN YFG+ ILSP TGI+  N  DDF        
Sbjct: 369 WNPIHDHGTSHLSVVDGERNAVSMTTTVNYYFGAVILSPSTGIILNNEMDDFSVPANVSA 428

Query: 427 ---PPAPSNFIRPGKRPLSSVSPTILLKDGELKAVVGASGGRFIIGAVAQVLLNHFTKGM 486
              PPAPSNFIRPGKRPLSS+SP I+LK+G+LK VVGASGG FII   A+V+LNHF KGM
Sbjct: 429 NKLPPAPSNFIRPGKRPLSSMSPAIVLKNGQLKGVVGASGGGFIIAGTAEVVLNHFAKGM 488

Query: 487 DPFKSVMTPRVYHTLFPNILTYENWTR-NEDRFEVSNEIVKFLATKNHKLKGVSGTTTVC 546
           DPF SVM PRVYH L PN+LTYENWT    D FEV  +    LA K H L+G++G  T+C
Sbjct: 489 DPFSSVMAPRVYHRLIPNVLTYENWTTVLGDHFEVPAQTRAALAKKGHVLEGLAG-GTIC 548

Query: 547 QFIVQDFNSP------GKLTAVSDPRKGGAPYGY 559
           QFIVQ+F SP      GKL AVSDPRKGGAP G+
Sbjct: 549 QFIVQNFGSPRQGGGVGKLIAVSDPRKGGAPAGF 573

BLAST of PI0005633 vs. ExPASy TrEMBL
Match: A0A6J1KR64 (glutathione hydrolase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496901 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 3.7e-183
Identity = 357/574 (62.20%), Postives = 431/574 (75.09%), Query Frame = 0

Query: 7   IVVVISTVLLLLPFTSIARPEVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVDAAVATA 66
           +++V+ ++ LL+P TSIARPEVI AS GAVAADD RCS+IG+ VL++  G+AVDA++A A
Sbjct: 21  LIIVLVSIALLIP-TSIARPEVIVASHGAVAADDSRCSRIGMDVLRE-GGHAVDASIAAA 80

Query: 67  LCQGVVSPASSGIGGGGFMLIRLANGESQVFDMREIAPLKAHERMYDGIPKAKKVGILSV 126
           LC GVVS ASSGIGGG FMLIR A+G+SQ FDMRE APL AHE +Y     +K  G LS+
Sbjct: 81  LCLGVVSSASSGIGGGAFMLIRSASGKSQAFDMRETAPLLAHENIYASNVASKAAGALSI 140

Query: 127 GVPGEVAGLYEAWRQYGNCSWADLVEPAMLLARNGFKISQNLHDQMSANESAITSDIGLA 186
            VPGE+AGL+EAW+QYG   WA LV+PA  LAR GFKIS  LH QM   E+ I  D GL 
Sbjct: 141 AVPGELAGLHEAWKQYGKLPWARLVQPAERLARLGFKISPYLHMQMVKTEAGILGDEGLR 200

Query: 187 SIFTKMNGTKRILLKSGETAYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDIEERKGI 246
           ++FT  +G K  LLK G+   NKKLA+TL TI+ +G  A Y N S+GI+LI+DI +  GI
Sbjct: 201 NLFT--SGGK--LLKPGDVIRNKKLAQTLRTISRFGVQAFY-NGSIGINLIRDIRKLGGI 260

Query: 247 VSMRDLRRYRVEIRKPIFSSTIAGLDIITAPPPSGGSGLILLLNILARYKVHD--RGPLS 306
           +SM+DL+ YRV+IR PI + T+ G +IIT PPPSGGSGLIL+LNILA+YK+     GPL 
Sbjct: 261 LSMKDLQSYRVKIRVPISARTM-GHEIITMPPPSGGSGLILMLNILAQYKLPSGLLGPLG 320

Query: 307 IHREIEALKHVIAVRTYLGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFDSNYYGGR 366
           IHREIEALKHV AVRT LGDP+FYN+S+VLSDMLSP+FA +L K INDN TFD ++YGGR
Sbjct: 321 IHREIEALKHVAAVRTNLGDPNFYNVSKVLSDMLSPKFARKLKKTINDNRTFDPSHYGGR 380

Query: 367 WKPNNEEGTTHLCVIDKERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF-------- 426
           W P ++ GT+HL V+D ERNAVS+T+TVN YFG+ ILSP TGI+  N  DDF        
Sbjct: 381 WNPIHDHGTSHLSVVDGERNAVSMTTTVNYYFGAVILSPSTGIILNNEMDDFSVPANVSA 440

Query: 427 ---PPAPSNFIRPGKRPLSSVSPTILLKDGELKAVVGASGGRFIIGAVAQVLLNHFTKGM 486
              PPAPSNFIRPGKRPLSS+SP I+LK+G+LK VVGASGG FII   A+V+LNHF KGM
Sbjct: 441 NKLPPAPSNFIRPGKRPLSSMSPAIVLKNGQLKGVVGASGGGFIIAGTAEVVLNHFAKGM 500

Query: 487 DPFKSVMTPRVYHTLFPNILTYENWTR-NEDRFEVSNEIVKFLATKNHKLKGVSGTTTVC 546
           DPF SVM PRVYH L PN+LTYENWT    D FEV  +    LA K H L+G++G  T+C
Sbjct: 501 DPFSSVMAPRVYHRLIPNVLTYENWTTVLGDHFEVPAQTRAALAKKGHVLEGLAG-GTIC 560

Query: 547 QFIVQDFNSP------GKLTAVSDPRKGGAPYGY 559
           QFIVQ+F SP      GKL AVSDPRKGGAP G+
Sbjct: 561 QFIVQNFGSPRQGGGVGKLIAVSDPRKGGAPAGF 585

BLAST of PI0005633 vs. NCBI nr
Match: XP_038883429.1 (glutathione hydrolase 1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 661.8 bits (1706), Expect = 5.7e-186
Identity = 362/567 (63.84%), Postives = 426/567 (75.13%), Query Frame = 0

Query: 8   VVVISTVLLLLPFTSIARPEVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVDAAVATAL 67
           +V++   + LLP  SIARPEVI AS GA+AADDHRCSKIG+ VL++  G+AVDA+VA AL
Sbjct: 14  LVIVLVSIGLLPPASIARPEVIVASHGAIAADDHRCSKIGMDVLRE-GGHAVDASVAAAL 73

Query: 68  CQGVVSPASSGIGGGGFMLIRLANGESQVFDMREIAPLKAHERMYDGIPKAKKVGILSVG 127
           C GVVSPASSGIGGG  MLIR A+G+SQ FDMRE APL A E MY G   +K  G LS+ 
Sbjct: 74  CLGVVSPASSGIGGGALMLIRSASGKSQAFDMRETAPLLASENMYTGNVASKATGALSIA 133

Query: 128 VPGEVAGLYEAWRQYGNCSWADLVEPAMLLARNGFKISQNLHDQMSANESAITSDIGLAS 187
           +PGE+AGL+EAW+Q+G   WA LV+PA  LAR GFKIS  LH QM   ES I  D GL +
Sbjct: 134 IPGELAGLHEAWKQHGKLPWARLVQPAERLARLGFKISPYLHMQMVRTESGILGDEGLRN 193

Query: 188 IFTKMNGTKRILLKSGETAYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDIEERKGIV 247
           + T  +G K  LLK G+   N KLA+TL TI+ +G  A Y N S+GI+LI+DI +  G++
Sbjct: 194 LLT--SGGK--LLKRGDVVRNMKLAKTLRTISRFGVKAFY-NGSIGINLIRDIRKLGGLL 253

Query: 248 SMRDLRRYRVEIRKPIFSSTIAGLDIITAPPPSGGSGLILLLNILARYKVHD--RGPLSI 307
           SM+DL+ YRV+IR PI S T+ GLDIIT PPPSGGSG+IL+LNILA+YK+     GPL I
Sbjct: 254 SMKDLQNYRVKIRVPILSHTM-GLDIITMPPPSGGSGVILMLNILAQYKLPSGLLGPLGI 313

Query: 308 HREIEALKHVIAVRTYLGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFDSNYYGGRW 367
           HREIEALKHV+AVRT LGDPDFYN+S+VLSDMLSP+FA++L K IND  TFD ++YGGRW
Sbjct: 314 HREIEALKHVVAVRTNLGDPDFYNVSKVLSDMLSPKFAQKLKKTINDKKTFDPSHYGGRW 373

Query: 368 KPNNEEGTTHLCVIDKERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF--------- 427
            P N+ GT+HL V+D ERNAVS+T+TVN YFG+HILSP TGIV  N  DDF         
Sbjct: 374 NPINDHGTSHLSVVDGERNAVSMTTTVNYYFGAHILSPSTGIVLNNEMDDFAIPANVSAN 433

Query: 428 --PPAPSNFIRPGKRPLSSVSPTILLKDGELKAVVGASGGRFIIGAVAQVLLNHFTKGMD 487
             PPAP NFI+PGKRPLSS+SPTI+LKDG+LKAVVGASGG FII   AQVLLNHF KGMD
Sbjct: 434 KLPPAPPNFIKPGKRPLSSMSPTIVLKDGQLKAVVGASGGGFIIAGTAQVLLNHFAKGMD 493

Query: 488 PFKSVMTPRVYHTLFPNILTYENWTR-NEDRFEVSNEIVKFLATKNHKLKGVSGTTTVCQ 547
           PF SVM PRVYH L PNIL YENWT   ED FEV  E    LA K H L+G++G  T+CQ
Sbjct: 494 PFSSVMAPRVYHPLIPNILNYENWTTVIEDHFEVPAETRAALAKKGHVLEGLAG-GTICQ 553

Query: 548 FIVQDFNSPGKLTAVSDPRKGGAPYGY 559
           FIVQ     GKL AVSDPRKGGAP G+
Sbjct: 554 FIVQG-GGLGKLFAVSDPRKGGAPAGF 571

BLAST of PI0005633 vs. NCBI nr
Match: XP_038883430.1 (glutathione hydrolase 1-like isoform X3 [Benincasa hispida])

HSP 1 Score: 661.8 bits (1706), Expect = 5.7e-186
Identity = 362/567 (63.84%), Postives = 426/567 (75.13%), Query Frame = 0

Query: 8   VVVISTVLLLLPFTSIARPEVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVDAAVATAL 67
           +V++   + LLP  SIARPEVI AS GA+AADDHRCSKIG+ VL++  G+AVDA+VA AL
Sbjct: 9   LVIVLVSIGLLPPASIARPEVIVASHGAIAADDHRCSKIGMDVLRE-GGHAVDASVAAAL 68

Query: 68  CQGVVSPASSGIGGGGFMLIRLANGESQVFDMREIAPLKAHERMYDGIPKAKKVGILSVG 127
           C GVVSPASSGIGGG  MLIR A+G+SQ FDMRE APL A E MY G   +K  G LS+ 
Sbjct: 69  CLGVVSPASSGIGGGALMLIRSASGKSQAFDMRETAPLLASENMYTGNVASKATGALSIA 128

Query: 128 VPGEVAGLYEAWRQYGNCSWADLVEPAMLLARNGFKISQNLHDQMSANESAITSDIGLAS 187
           +PGE+AGL+EAW+Q+G   WA LV+PA  LAR GFKIS  LH QM   ES I  D GL +
Sbjct: 129 IPGELAGLHEAWKQHGKLPWARLVQPAERLARLGFKISPYLHMQMVRTESGILGDEGLRN 188

Query: 188 IFTKMNGTKRILLKSGETAYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDIEERKGIV 247
           + T  +G K  LLK G+   N KLA+TL TI+ +G  A Y N S+GI+LI+DI +  G++
Sbjct: 189 LLT--SGGK--LLKRGDVVRNMKLAKTLRTISRFGVKAFY-NGSIGINLIRDIRKLGGLL 248

Query: 248 SMRDLRRYRVEIRKPIFSSTIAGLDIITAPPPSGGSGLILLLNILARYKVHD--RGPLSI 307
           SM+DL+ YRV+IR PI S T+ GLDIIT PPPSGGSG+IL+LNILA+YK+     GPL I
Sbjct: 249 SMKDLQNYRVKIRVPILSHTM-GLDIITMPPPSGGSGVILMLNILAQYKLPSGLLGPLGI 308

Query: 308 HREIEALKHVIAVRTYLGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFDSNYYGGRW 367
           HREIEALKHV+AVRT LGDPDFYN+S+VLSDMLSP+FA++L K IND  TFD ++YGGRW
Sbjct: 309 HREIEALKHVVAVRTNLGDPDFYNVSKVLSDMLSPKFAQKLKKTINDKKTFDPSHYGGRW 368

Query: 368 KPNNEEGTTHLCVIDKERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF--------- 427
            P N+ GT+HL V+D ERNAVS+T+TVN YFG+HILSP TGIV  N  DDF         
Sbjct: 369 NPINDHGTSHLSVVDGERNAVSMTTTVNYYFGAHILSPSTGIVLNNEMDDFAIPANVSAN 428

Query: 428 --PPAPSNFIRPGKRPLSSVSPTILLKDGELKAVVGASGGRFIIGAVAQVLLNHFTKGMD 487
             PPAP NFI+PGKRPLSS+SPTI+LKDG+LKAVVGASGG FII   AQVLLNHF KGMD
Sbjct: 429 KLPPAPPNFIKPGKRPLSSMSPTIVLKDGQLKAVVGASGGGFIIAGTAQVLLNHFAKGMD 488

Query: 488 PFKSVMTPRVYHTLFPNILTYENWTR-NEDRFEVSNEIVKFLATKNHKLKGVSGTTTVCQ 547
           PF SVM PRVYH L PNIL YENWT   ED FEV  E    LA K H L+G++G  T+CQ
Sbjct: 489 PFSSVMAPRVYHPLIPNILNYENWTTVIEDHFEVPAETRAALAKKGHVLEGLAG-GTICQ 548

Query: 548 FIVQDFNSPGKLTAVSDPRKGGAPYGY 559
           FIVQ     GKL AVSDPRKGGAP G+
Sbjct: 549 FIVQG-GGLGKLFAVSDPRKGGAPAGF 566

BLAST of PI0005633 vs. NCBI nr
Match: XP_038883431.1 (glutathione hydrolase 1-like isoform X4 [Benincasa hispida] >XP_038883432.1 glutathione hydrolase 1-like isoform X4 [Benincasa hispida] >XP_038883433.1 glutathione hydrolase 1-like isoform X4 [Benincasa hispida] >XP_038883434.1 glutathione hydrolase 1-like isoform X4 [Benincasa hispida] >XP_038883435.1 glutathione hydrolase 1-like isoform X4 [Benincasa hispida] >XP_038883436.1 glutathione hydrolase 1-like isoform X4 [Benincasa hispida])

HSP 1 Score: 661.8 bits (1706), Expect = 5.7e-186
Identity = 362/567 (63.84%), Postives = 426/567 (75.13%), Query Frame = 0

Query: 8   VVVISTVLLLLPFTSIARPEVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVDAAVATAL 67
           +V++   + LLP  SIARPEVI AS GA+AADDHRCSKIG+ VL++  G+AVDA+VA AL
Sbjct: 6   LVIVLVSIGLLPPASIARPEVIVASHGAIAADDHRCSKIGMDVLRE-GGHAVDASVAAAL 65

Query: 68  CQGVVSPASSGIGGGGFMLIRLANGESQVFDMREIAPLKAHERMYDGIPKAKKVGILSVG 127
           C GVVSPASSGIGGG  MLIR A+G+SQ FDMRE APL A E MY G   +K  G LS+ 
Sbjct: 66  CLGVVSPASSGIGGGALMLIRSASGKSQAFDMRETAPLLASENMYTGNVASKATGALSIA 125

Query: 128 VPGEVAGLYEAWRQYGNCSWADLVEPAMLLARNGFKISQNLHDQMSANESAITSDIGLAS 187
           +PGE+AGL+EAW+Q+G   WA LV+PA  LAR GFKIS  LH QM   ES I  D GL +
Sbjct: 126 IPGELAGLHEAWKQHGKLPWARLVQPAERLARLGFKISPYLHMQMVRTESGILGDEGLRN 185

Query: 188 IFTKMNGTKRILLKSGETAYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDIEERKGIV 247
           + T  +G K  LLK G+   N KLA+TL TI+ +G  A Y N S+GI+LI+DI +  G++
Sbjct: 186 LLT--SGGK--LLKRGDVVRNMKLAKTLRTISRFGVKAFY-NGSIGINLIRDIRKLGGLL 245

Query: 248 SMRDLRRYRVEIRKPIFSSTIAGLDIITAPPPSGGSGLILLLNILARYKVHD--RGPLSI 307
           SM+DL+ YRV+IR PI S T+ GLDIIT PPPSGGSG+IL+LNILA+YK+     GPL I
Sbjct: 246 SMKDLQNYRVKIRVPILSHTM-GLDIITMPPPSGGSGVILMLNILAQYKLPSGLLGPLGI 305

Query: 308 HREIEALKHVIAVRTYLGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFDSNYYGGRW 367
           HREIEALKHV+AVRT LGDPDFYN+S+VLSDMLSP+FA++L K IND  TFD ++YGGRW
Sbjct: 306 HREIEALKHVVAVRTNLGDPDFYNVSKVLSDMLSPKFAQKLKKTINDKKTFDPSHYGGRW 365

Query: 368 KPNNEEGTTHLCVIDKERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF--------- 427
            P N+ GT+HL V+D ERNAVS+T+TVN YFG+HILSP TGIV  N  DDF         
Sbjct: 366 NPINDHGTSHLSVVDGERNAVSMTTTVNYYFGAHILSPSTGIVLNNEMDDFAIPANVSAN 425

Query: 428 --PPAPSNFIRPGKRPLSSVSPTILLKDGELKAVVGASGGRFIIGAVAQVLLNHFTKGMD 487
             PPAP NFI+PGKRPLSS+SPTI+LKDG+LKAVVGASGG FII   AQVLLNHF KGMD
Sbjct: 426 KLPPAPPNFIKPGKRPLSSMSPTIVLKDGQLKAVVGASGGGFIIAGTAQVLLNHFAKGMD 485

Query: 488 PFKSVMTPRVYHTLFPNILTYENWTR-NEDRFEVSNEIVKFLATKNHKLKGVSGTTTVCQ 547
           PF SVM PRVYH L PNIL YENWT   ED FEV  E    LA K H L+G++G  T+CQ
Sbjct: 486 PFSSVMAPRVYHPLIPNILNYENWTTVIEDHFEVPAETRAALAKKGHVLEGLAG-GTICQ 545

Query: 548 FIVQDFNSPGKLTAVSDPRKGGAPYGY 559
           FIVQ     GKL AVSDPRKGGAP G+
Sbjct: 546 FIVQG-GGLGKLFAVSDPRKGGAPAGF 563

BLAST of PI0005633 vs. NCBI nr
Match: XP_038883428.1 (glutathione hydrolase 1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 661.8 bits (1706), Expect = 5.7e-186
Identity = 362/567 (63.84%), Postives = 426/567 (75.13%), Query Frame = 0

Query: 8   VVVISTVLLLLPFTSIARPEVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVDAAVATAL 67
           +V++   + LLP  SIARPEVI AS GA+AADDHRCSKIG+ VL++  G+AVDA+VA AL
Sbjct: 17  LVIVLVSIGLLPPASIARPEVIVASHGAIAADDHRCSKIGMDVLRE-GGHAVDASVAAAL 76

Query: 68  CQGVVSPASSGIGGGGFMLIRLANGESQVFDMREIAPLKAHERMYDGIPKAKKVGILSVG 127
           C GVVSPASSGIGGG  MLIR A+G+SQ FDMRE APL A E MY G   +K  G LS+ 
Sbjct: 77  CLGVVSPASSGIGGGALMLIRSASGKSQAFDMRETAPLLASENMYTGNVASKATGALSIA 136

Query: 128 VPGEVAGLYEAWRQYGNCSWADLVEPAMLLARNGFKISQNLHDQMSANESAITSDIGLAS 187
           +PGE+AGL+EAW+Q+G   WA LV+PA  LAR GFKIS  LH QM   ES I  D GL +
Sbjct: 137 IPGELAGLHEAWKQHGKLPWARLVQPAERLARLGFKISPYLHMQMVRTESGILGDEGLRN 196

Query: 188 IFTKMNGTKRILLKSGETAYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDIEERKGIV 247
           + T  +G K  LLK G+   N KLA+TL TI+ +G  A Y N S+GI+LI+DI +  G++
Sbjct: 197 LLT--SGGK--LLKRGDVVRNMKLAKTLRTISRFGVKAFY-NGSIGINLIRDIRKLGGLL 256

Query: 248 SMRDLRRYRVEIRKPIFSSTIAGLDIITAPPPSGGSGLILLLNILARYKVHD--RGPLSI 307
           SM+DL+ YRV+IR PI S T+ GLDIIT PPPSGGSG+IL+LNILA+YK+     GPL I
Sbjct: 257 SMKDLQNYRVKIRVPILSHTM-GLDIITMPPPSGGSGVILMLNILAQYKLPSGLLGPLGI 316

Query: 308 HREIEALKHVIAVRTYLGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFDSNYYGGRW 367
           HREIEALKHV+AVRT LGDPDFYN+S+VLSDMLSP+FA++L K IND  TFD ++YGGRW
Sbjct: 317 HREIEALKHVVAVRTNLGDPDFYNVSKVLSDMLSPKFAQKLKKTINDKKTFDPSHYGGRW 376

Query: 368 KPNNEEGTTHLCVIDKERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF--------- 427
            P N+ GT+HL V+D ERNAVS+T+TVN YFG+HILSP TGIV  N  DDF         
Sbjct: 377 NPINDHGTSHLSVVDGERNAVSMTTTVNYYFGAHILSPSTGIVLNNEMDDFAIPANVSAN 436

Query: 428 --PPAPSNFIRPGKRPLSSVSPTILLKDGELKAVVGASGGRFIIGAVAQVLLNHFTKGMD 487
             PPAP NFI+PGKRPLSS+SPTI+LKDG+LKAVVGASGG FII   AQVLLNHF KGMD
Sbjct: 437 KLPPAPPNFIKPGKRPLSSMSPTIVLKDGQLKAVVGASGGGFIIAGTAQVLLNHFAKGMD 496

Query: 488 PFKSVMTPRVYHTLFPNILTYENWTR-NEDRFEVSNEIVKFLATKNHKLKGVSGTTTVCQ 547
           PF SVM PRVYH L PNIL YENWT   ED FEV  E    LA K H L+G++G  T+CQ
Sbjct: 497 PFSSVMAPRVYHPLIPNILNYENWTTVIEDHFEVPAETRAALAKKGHVLEGLAG-GTICQ 556

Query: 548 FIVQDFNSPGKLTAVSDPRKGGAPYGY 559
           FIVQ     GKL AVSDPRKGGAP G+
Sbjct: 557 FIVQG-GGLGKLFAVSDPRKGGAPAGF 574

BLAST of PI0005633 vs. NCBI nr
Match: KAG6594851.1 (Glutathione hydrolase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 654.8 bits (1688), Expect = 6.9e-184
Identity = 360/574 (62.72%), Postives = 431/574 (75.09%), Query Frame = 0

Query: 7   IVVVISTVLLLLPFTSIARPEVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVDAAVATA 66
           +++V+ ++ LL P TSIARPEVI AS GAVAADD RCS+IG+ VL++  G+AVDA+VATA
Sbjct: 52  LIIVLVSIALLTP-TSIARPEVIVASHGAVAADDSRCSRIGMDVLRE-GGHAVDASVATA 111

Query: 67  LCQGVVSPASSGIGGGGFMLIRLANGESQVFDMREIAPLKAHERMYDGIPKAKKVGILSV 126
           LC GVVS ASSGIGGG FMLIR A+G+SQ FDMRE APL AHE MY     +K VG LS+
Sbjct: 112 LCLGVVSSASSGIGGGAFMLIRSASGKSQAFDMRETAPLLAHENMYASNAASKAVGALSI 171

Query: 127 GVPGEVAGLYEAWRQYGNCSWADLVEPAMLLARNGFKISQNLHDQMSANESAITSDIGLA 186
            VPGE+AGL+EAW+QYG   WA LV+PA  LAR GFKIS  LH QM   E+ I  D GL 
Sbjct: 172 AVPGELAGLHEAWKQYGKLPWARLVQPAERLARLGFKISPYLHMQMVKTEAGILGDEGLR 231

Query: 187 SIFTKMNGTKRILLKSGETAYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDIEERKGI 246
           ++FT  +G K  LLK G+   NKKLA+TL TI+ +G  A Y N S+G++LI+DI +  GI
Sbjct: 232 NLFT--SGGK--LLKPGDVIRNKKLAQTLRTISRFGVQAFY-NGSIGVNLIRDIRKLGGI 291

Query: 247 VSMRDLRRYRVEIRKPIFSSTIAGLDIITAPPPSGGSGLILLLNILARYKVHD--RGPLS 306
           +SM+DL+ YRV+IR PI + T+ G +IIT PPPSGGSGLIL+LNILA+YK+     GPL 
Sbjct: 292 LSMKDLQSYRVKIRVPISARTM-GHEIITMPPPSGGSGLILMLNILAQYKLPSGLMGPLG 351

Query: 307 IHREIEALKHVIAVRTYLGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFDSNYYGGR 366
           IHREIEALKHV AVRT LGDP+FYN+S+VLSDMLSP+FA +L K INDN TFD ++YGGR
Sbjct: 352 IHREIEALKHVAAVRTNLGDPNFYNVSKVLSDMLSPKFARKLKKTINDNRTFDPSHYGGR 411

Query: 367 WKPNNEEGTTHLCVIDKERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF-------- 426
           W P  + GT+HL V+D ERNAVS+T+TVN YFG+ ILSP TGI+  N  DDF        
Sbjct: 412 WNPIYDHGTSHLSVVDGERNAVSMTTTVNYYFGAVILSPSTGIILNNEMDDFSVPANVSA 471

Query: 427 ---PPAPSNFIRPGKRPLSSVSPTILLKDGELKAVVGASGGRFIIGAVAQVLLNHFTKGM 486
              PPAPSNFIRPGKRPLSS+SP I+LK+G+LK VVGASGG FII   A+V+LNHF KGM
Sbjct: 472 NTLPPAPSNFIRPGKRPLSSMSPAIVLKNGQLKGVVGASGGGFIIAGTAEVVLNHFAKGM 531

Query: 487 DPFKSVMTPRVYHTLFPNILTYENWTR-NEDRFEVSNEIVKFLATKNHKLKGVSGTTTVC 546
           DPF SVM PRVYH L PN+LTYENWT    D FEV  +    LA K H L+G++G  T+C
Sbjct: 532 DPFSSVMAPRVYHRLIPNVLTYENWTTVLGDHFEVPAQTRAALAKKGHVLEGLAG-GTIC 591

Query: 547 QFIVQDFNSP------GKLTAVSDPRKGGAPYGY 559
           QFIVQ+F SP      GKL AVSDPRKGGAP G+
Sbjct: 592 QFIVQNFGSPRQGGGVGKLIAVSDPRKGGAPAGF 616

BLAST of PI0005633 vs. TAIR 10
Match: AT4G39650.1 (gamma-glutamyl transpeptidase 2 )

HSP 1 Score: 533.5 bits (1373), Expect = 2.2e-151
Identity = 306/586 (52.22%), Postives = 384/586 (65.53%), Query Frame = 0

Query: 1   MNYNKVIVVVISTVLLLLPF----TSIARPEVIEASLGAVAADDHRCSKIGVYVLQKLKG 60
           MN    +V   +  LLL+ F     ++   + I A  GAVA DD RCS IG  VL++  G
Sbjct: 1   MNSFMSLVRTATIALLLIAFLQNANAVKNLQSIVAYHGAVATDDGRCSAIGTNVLRQ-GG 60

Query: 61  NAVDAAVATALCQGVVSPASSGIGGGGFMLIRLANGESQVFDMREIAPLKAHERMYDGIP 120
           NA+DA+VA ALC GVVSPASSGIGGG F +I+LANG    +D RE APL A E MY   P
Sbjct: 61  NAIDASVAAALCLGVVSPASSGIGGGAFTMIKLANGTEVAYDSRETAPLSATEDMYGDNP 120

Query: 121 KAKKVGILSVGVPGEVAGLYEAWRQYGNCSWADLVEPAMLLARNGFKISQNLHDQMSANE 180
           + KK G LSVGVPGEVAGLY AW Q+G   W  LVEPA  LA  GFKIS+ L+ QM+A  
Sbjct: 121 ERKKKGSLSVGVPGEVAGLYTAWTQHGKLPWKQLVEPAEKLAAEGFKISKYLYMQMNATR 180

Query: 181 SAITSDIGLASIFTKMNGTKRILLKSGETAYNKKLAETLETIAEYGAGALYDNKSVGIDL 240
           S I +D GL+ +F   NG    L K G    N KLA+TL  IAEYG  A Y N +VG +L
Sbjct: 181 SDILADKGLSELFVS-NGE---LKKPGAICRNPKLADTLSQIAEYGPKAFY-NGTVGFNL 240

Query: 241 IKDIEERKGIVSMRDLRRYRVEIRKPIFSSTIAGLDIITAPPP-SGGSGLILLLNILARY 300
           + DI++  GI++++DL+ Y V++++P+ S+ I G  ++  PPP SGG  ++L+LNILA+Y
Sbjct: 241 VSDIQKAGGIITLKDLQNYNVKVKEPL-STEILGYRLLGMPPPSSGGPAMMLILNILAQY 300

Query: 301 KVHD--RGPLSIHREIEALKHVIAVRTYLGDPDFY-NISQVLSDMLSPQFAEELHKAIND 360
            +     GPL +HR +EALKH  AVR  LGDPDF   ++ V++DMLSP+FA++L   IND
Sbjct: 301 GIPSGVSGPLGVHRLVEALKHAFAVRMNLGDPDFVPEVTNVVADMLSPKFAQDLKSKIND 360

Query: 361 NTTFDSNYYGGRWKPNNEEGTTHLCVIDKERNAVSLTSTVNNYFGSHILSPRTGIV--NT 420
             TFD  YYGG+W    + GT+HL +ID ERNAVS+TST+N YFG+ +LSP TGIV  N 
Sbjct: 361 EKTFDPKYYGGKWGQIKDHGTSHLSIIDSERNAVSMTSTINGYFGAIMLSPSTGIVLNNE 420

Query: 421 ADDF-------------PPAPSNFIRPGKRPLSSVSPTILLKDGELKAVVGASGGRFIIG 480
            DDF             PPAP+NFIRPGKRPLSS++PTI+LKDG++KA +GASGG +II 
Sbjct: 421 MDDFSIPTKSGGDPDVPPPAPANFIRPGKRPLSSMTPTIVLKDGKVKAALGASGGMYIIA 480

Query: 481 AVAQVLLNHFTKGMDPFKSVMTPRVYHTLFPNILTYENWTR-NEDRFEVSNEIVKFLATK 540
              QV LNHF   MDP  SV+ PR+YH L PN  +YENWT    D FE+  EI   L  K
Sbjct: 481 GTTQVYLNHFFLNMDPLSSVVAPRIYHQLIPNKASYENWTTVYSDHFEIPEEIRLVLEKK 540

Query: 541 NHKLKGVSGTTTVCQFIVQ--DFNSPG--KLTAVSDPRKGGAPYGY 559
              L  ++G  T+ Q IV+  D  S G  KL AVSDPRKGG P GY
Sbjct: 541 GQVLTPIAG-GTISQLIVEQSDGKSGGISKLVAVSDPRKGGFPSGY 578

BLAST of PI0005633 vs. TAIR 10
Match: AT4G39640.1 (gamma-glutamyl transpeptidase 1 )

HSP 1 Score: 533.5 bits (1373), Expect = 2.2e-151
Identity = 301/581 (51.81%), Postives = 390/581 (67.13%), Query Frame = 0

Query: 1   MNYNKVIVVVISTVLLLLPFTSIARPEVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVD 60
           M+  + + +V+  +  L    +  R + I  S GAVA DD RCS IG+ VL++  GNA+D
Sbjct: 1   MSLVRTVTIVLFIIAFLQNAAAQKRQQSIVKSRGAVATDDGRCSVIGMRVLRE-GGNAID 60

Query: 61  AAVATALCQGVVSPASSGIGGGGFMLIRLANGESQVFDMREIAPLKAHERMYDGIPKAKK 120
           A+VA ALC GVVSPASSGIGGG F ++++A G+   +D RE APL+A E MY G    KK
Sbjct: 61  ASVAAALCLGVVSPASSGIGGGAFTVVKIAGGKEIAYDSRETAPLRATENMYGGNVDLKK 120

Query: 121 VGILSVGVPGEVAGLYEAWRQYGNCSWADLVEPAMLLARNGFKISQNLHDQMSANESAIT 180
            G LSVGVPGEVAGL+ AW+Q+G   W  LV PA  LA  GFKIS+ L+ QM+A  S I 
Sbjct: 121 KGALSVGVPGEVAGLFTAWKQHGKLPWKRLVTPAEKLA-EGFKISKYLYMQMNATRSDIL 180

Query: 181 SDIGLASIFTKMNGTKRILLKSGETAYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDI 240
           +D GL+ +F   NG    L K G   +N KLA TL+ I EYG  A Y N +VG++L +DI
Sbjct: 181 ADKGLSDLFVS-NGE---LKKPGTICHNPKLALTLKLIGEYGPKAFY-NGTVGVNLARDI 240

Query: 241 EERKGIVSMRDLRRYRVEIRKPIFSSTIAGLDIITAPPP-SGGSGLILLLNILARYKVHD 300
           ++  GI++++DL+ YRV+I++P+ S+ I G  ++  PPP SGG+ ++L+LNIL++Y +  
Sbjct: 241 KKSGGIITLKDLQSYRVKIKEPL-SADILGYRVLGMPPPSSGGAAMMLVLNILSQYGIPS 300

Query: 301 --RGPLSIHREIEALKHVIAVRTYLGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFD 360
              GPL +HR IEALKH  AVR  LGDPDF ++++V+SDMLSP+FA++L   IND  TFD
Sbjct: 301 GVSGPLGVHRLIEALKHAFAVRMNLGDPDFTDVTKVVSDMLSPKFAKDLKSKINDQKTFD 360

Query: 361 SNYYGGRWKPNNEEGTTHLCVIDKERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF- 420
             YYGG W   ++ GT+HL +ID+ERNAVS+TST+N YFG+ +LSP TGIV  N  DDF 
Sbjct: 361 PKYYGGMWNQIDDHGTSHLSIIDRERNAVSMTSTINGYFGALMLSPSTGIVLNNEMDDFS 420

Query: 421 ------------PPAPSNFIRPGKRPLSSVSPTILLKDGELKAVVGASGGRFIIGAVAQV 480
                       PPAP+NFIRPGKRPLSS+SPTI+LKDG++KA VGASGG  II    +V
Sbjct: 421 IPMKSNGNLDVPPPAPANFIRPGKRPLSSMSPTIVLKDGKVKAAVGASGGANIIAGTTEV 480

Query: 481 LLNHFTKGMDPFKSVMTPRVYHTLFPNILTYENWTR-NEDRFEVSNEIVKFLATKNHKLK 540
            LNHF   MDP  SV+ PR+YH L PN  +YENWT    D FE+       L  K H L 
Sbjct: 481 YLNHFFLKMDPLSSVLAPRIYHQLIPNRASYENWTTVFNDHFEIPKATRVVLEKKGHVLS 540

Query: 541 GVSGTTTVCQFIVQDF--NSPGK--LTAVSDPRKGGAPYGY 559
            ++G  T+ QFIVQ+   NS G+  L AVSDPRKGG P GY
Sbjct: 541 PIAG-GTIAQFIVQESGENSGGRSELVAVSDPRKGGFPSGY 572

BLAST of PI0005633 vs. TAIR 10
Match: AT4G39640.2 (gamma-glutamyl transpeptidase 1 )

HSP 1 Score: 533.5 bits (1373), Expect = 2.2e-151
Identity = 301/581 (51.81%), Postives = 390/581 (67.13%), Query Frame = 0

Query: 1   MNYNKVIVVVISTVLLLLPFTSIARPEVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVD 60
           M+  + + +V+  +  L    +  R + I  S GAVA DD RCS IG+ VL++  GNA+D
Sbjct: 1   MSLVRTVTIVLFIIAFLQNAAAQKRQQSIVKSRGAVATDDGRCSVIGMRVLRE-GGNAID 60

Query: 61  AAVATALCQGVVSPASSGIGGGGFMLIRLANGESQVFDMREIAPLKAHERMYDGIPKAKK 120
           A+VA ALC GVVSPASSGIGGG F ++++A G+   +D RE APL+A E MY G    KK
Sbjct: 61  ASVAAALCLGVVSPASSGIGGGAFTVVKIAGGKEIAYDSRETAPLRATENMYGGNVDLKK 120

Query: 121 VGILSVGVPGEVAGLYEAWRQYGNCSWADLVEPAMLLARNGFKISQNLHDQMSANESAIT 180
            G LSVGVPGEVAGL+ AW+Q+G   W  LV PA  LA  GFKIS+ L+ QM+A  S I 
Sbjct: 121 KGALSVGVPGEVAGLFTAWKQHGKLPWKRLVTPAEKLA-EGFKISKYLYMQMNATRSDIL 180

Query: 181 SDIGLASIFTKMNGTKRILLKSGETAYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDI 240
           +D GL+ +F   NG    L K G   +N KLA TL+ I EYG  A Y N +VG++L +DI
Sbjct: 181 ADKGLSDLFVS-NGE---LKKPGTICHNPKLALTLKLIGEYGPKAFY-NGTVGVNLARDI 240

Query: 241 EERKGIVSMRDLRRYRVEIRKPIFSSTIAGLDIITAPPP-SGGSGLILLLNILARYKVHD 300
           ++  GI++++DL+ YRV+I++P+ S+ I G  ++  PPP SGG+ ++L+LNIL++Y +  
Sbjct: 241 KKSGGIITLKDLQSYRVKIKEPL-SADILGYRVLGMPPPSSGGAAMMLVLNILSQYGIPS 300

Query: 301 --RGPLSIHREIEALKHVIAVRTYLGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFD 360
              GPL +HR IEALKH  AVR  LGDPDF ++++V+SDMLSP+FA++L   IND  TFD
Sbjct: 301 GVSGPLGVHRLIEALKHAFAVRMNLGDPDFTDVTKVVSDMLSPKFAKDLKSKINDQKTFD 360

Query: 361 SNYYGGRWKPNNEEGTTHLCVIDKERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF- 420
             YYGG W   ++ GT+HL +ID+ERNAVS+TST+N YFG+ +LSP TGIV  N  DDF 
Sbjct: 361 PKYYGGMWNQIDDHGTSHLSIIDRERNAVSMTSTINGYFGALMLSPSTGIVLNNEMDDFS 420

Query: 421 ------------PPAPSNFIRPGKRPLSSVSPTILLKDGELKAVVGASGGRFIIGAVAQV 480
                       PPAP+NFIRPGKRPLSS+SPTI+LKDG++KA VGASGG  II    +V
Sbjct: 421 IPMKSNGNLDVPPPAPANFIRPGKRPLSSMSPTIVLKDGKVKAAVGASGGANIIAGTTEV 480

Query: 481 LLNHFTKGMDPFKSVMTPRVYHTLFPNILTYENWTR-NEDRFEVSNEIVKFLATKNHKLK 540
            LNHF   MDP  SV+ PR+YH L PN  +YENWT    D FE+       L  K H L 
Sbjct: 481 YLNHFFLKMDPLSSVLAPRIYHQLIPNRASYENWTTVFNDHFEIPKATRVVLEKKGHVLS 540

Query: 541 GVSGTTTVCQFIVQDF--NSPGK--LTAVSDPRKGGAPYGY 559
            ++G  T+ QFIVQ+   NS G+  L AVSDPRKGG P GY
Sbjct: 541 PIAG-GTIAQFIVQESGENSGGRSELVAVSDPRKGGFPSGY 572

BLAST of PI0005633 vs. TAIR 10
Match: AT4G29210.1 (gamma-glutamyl transpeptidase 4 )

HSP 1 Score: 468.0 bits (1203), Expect = 1.1e-131
Identity = 267/569 (46.92%), Postives = 355/569 (62.39%), Query Frame = 0

Query: 27  EVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVDAAVATALCQGVVSPASSGIGGGGFML 86
           +V+E+  G VAADD RCS+IG  VL+   G+AVDAAVA  LC GVV+P SSGIGGG F++
Sbjct: 74  DVVESENGVVAADDARCSEIGASVLRS-GGHAVDAAVAITLCVGVVNPMSSGIGGGSFLI 133

Query: 87  I-RLANGESQVFDMREIAPLKAHERMYDGIPKAKKVGILSVGVPGEVAGLYEAWRQYGNC 146
           +    + +++ FDMRE APL A + MY     AK +G LS+GVPGE+AGLYEAW++YG  
Sbjct: 134 VSSQKDSKAEAFDMRETAPLAASKDMYKNDASAKSLGALSMGVPGEIAGLYEAWKRYGRL 193

Query: 147 SWADLVEPAMLLARNGFKISQNLHDQMSANESAITSDIGLASIFTKMNGTKRILLKSGET 206
            W  L EPA+ LAR+GF +   L   +S   + I  D G+ S+F++ NG    +LK+GET
Sbjct: 194 PWKPLFEPAIKLARDGFVVYPYLGKAISTKVAMILKDPGMRSVFSR-NGQ---VLKTGET 253

Query: 207 AYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDIEERKGIVSMRDLRRYRVEIRKPIFS 266
            YN +LA++LETI+E G GA Y N +VG  L+KD+++  GI++M DLR Y+V +   + S
Sbjct: 254 CYNPELAQSLETISEQGPGAFY-NGTVGEKLVKDVKKAGGIITMDDLRSYKVRVTDAM-S 313

Query: 267 STIAGLDIITAPPPSGGS-GLILLLNILARYK---VHDRGPLSIHREIEALKHVIAVRTY 326
             + G  +   PPPSGG+ G  +++NIL  Y          L +HR IEA+KH+ A R  
Sbjct: 314 VDVMGYTVHGMPPPSGGTVGFSMVMNILDSYSNLYTASGRELGLHRLIEAMKHMFAARMD 373

Query: 327 LGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFDSNYYGGRWKPNNEEGTTHLCVIDK 386
           LGDP+F N++  ++ MLS   AEE+ K I DNTTF   YY  RW    ++GT+H CV+D 
Sbjct: 374 LGDPEFVNVTNSMNQMLSKAHAEEIQKRIFDNTTFPPEYYMNRWSQLRDQGTSHFCVVDA 433

Query: 387 ERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF-----------PPAPSNFIRPGKRP 446
           +RN+VS+TSTVN  FG+ +LSP TGIV  N  DDF           PPAP+NFI P KRP
Sbjct: 434 DRNSVSMTSTVNYRFGAGVLSPSTGIVLNNEMDDFSTPTEITPDMLPPAPTNFIEPNKRP 493

Query: 447 LSSVSPTILLKDGELKAVVGASGGRFIIGAVAQVLLNHFTKGMDPFKSVMTPRVYHTLFP 506
           LSS++P ++ KDGE  A +G +GG  II AV QV LN F   M P ++V + R+YH L P
Sbjct: 494 LSSMTPLVITKDGEFVAALGGAGGMHIIPAVLQVFLNCFVLNMKPKEAVESARIYHRLIP 553

Query: 507 NILTYENWTR-NEDRFEVSNEIVKFLATKNHKLKGVSGTTTVCQFIVQDFNS-------- 556
           N+++YEN+T  N D   VS +   FLA + H+LK +SG   V Q IVQ F          
Sbjct: 554 NVVSYENFTTINGDHIGVSEDTKMFLAERGHELKELSGGAIV-QLIVQSFKEEKEEEMII 613

BLAST of PI0005633 vs. TAIR 10
Match: AT4G29210.2 (gamma-glutamyl transpeptidase 4 )

HSP 1 Score: 377.9 bits (969), Expect = 1.5e-104
Identity = 208/431 (48.26%), Postives = 282/431 (65.43%), Query Frame = 0

Query: 27  EVIEASLGAVAADDHRCSKIGVYVLQKLKGNAVDAAVATALCQGVVSPASSGIGGGGFML 86
           +V+E+  G VAADD RCS+IG  VL+   G+AVDAAVA  LC GVV+P SSGIGGG F++
Sbjct: 74  DVVESENGVVAADDARCSEIGASVLRS-GGHAVDAAVAITLCVGVVNPMSSGIGGGSFLI 133

Query: 87  I-RLANGESQVFDMREIAPLKAHERMYDGIPKAKKVGILSVGVPGEVAGLYEAWRQYGNC 146
           +    + +++ FDMRE APL A + MY     AK +G LS+GVPGE+AGLYEAW++YG  
Sbjct: 134 VSSQKDSKAEAFDMRETAPLAASKDMYKNDASAKSLGALSMGVPGEIAGLYEAWKRYGRL 193

Query: 147 SWADLVEPAMLLARNGFKISQNLHDQMSANESAITSDIGLASIFTKMNGTKRILLKSGET 206
            W  L EPA+ LAR+GF +   L   +S   + I  D G+ S+F++ NG    +LK+GET
Sbjct: 194 PWKPLFEPAIKLARDGFVVYPYLGKAISTKVAMILKDPGMRSVFSR-NGQ---VLKTGET 253

Query: 207 AYNKKLAETLETIAEYGAGALYDNKSVGIDLIKDIEERKGIVSMRDLRRYRVEIRKPIFS 266
            YN +LA++LETI+E G GA Y N +VG  L+KD+++  GI++M DLR Y+V +   + S
Sbjct: 254 CYNPELAQSLETISEQGPGAFY-NGTVGEKLVKDVKKAGGIITMDDLRSYKVRVTDAM-S 313

Query: 267 STIAGLDIITAPPPSGGS-GLILLLNILARYK---VHDRGPLSIHREIEALKHVIAVRTY 326
             + G  +   PPPSGG+ G  +++NIL  Y          L +HR IEA+KH+ A R  
Sbjct: 314 VDVMGYTVHGMPPPSGGTVGFSMVMNILDSYSNLYTASGRELGLHRLIEAMKHMFAARMD 373

Query: 327 LGDPDFYNISQVLSDMLSPQFAEELHKAINDNTTFDSNYYGGRWKPNNEEGTTHLCVIDK 386
           LGDP+F N++  ++ MLS   AEE+ K I DNTTF   YY  RW    ++GT+H CV+D 
Sbjct: 374 LGDPEFVNVTNSMNQMLSKAHAEEIQKRIFDNTTFPPEYYMNRWSQLRDQGTSHFCVVDA 433

Query: 387 ERNAVSLTSTVNNYFGSHILSPRTGIV--NTADDF-----------PPAPSNFIRPGKRP 440
           +RN+VS+TSTVN  FG+ +LSP TGIV  N  DDF           PPAP+NFI P KRP
Sbjct: 434 DRNSVSMTSTVNYRFGAGVLSPSTGIVLNNEMDDFSTPTEITPDMLPPAPTNFIEPNKRP 493

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYW63.1e-15051.81Glutathione hydrolase 1 OS=Arabidopsis thaliana OX=3702 GN=GGT1 PE=2 SV=1[more]
Q680I53.1e-15052.22Glutathione hydrolase 2 OS=Arabidopsis thaliana OX=3702 GN=GGT2 PE=2 SV=1[more]
Q9M0G01.6e-13046.92Glutathione hydrolase 3 OS=Arabidopsis thaliana OX=3702 GN=GGT3 PE=2 SV=1[more]
Q9US044.3e-8836.41Glutathione hydrolase proenzyme 1 OS=Schizosaccharomyces pombe (strain 972 / ATC... [more]
Q609281.4e-8636.70Glutathione hydrolase 1 proenzyme OS=Mus musculus OX=10090 GN=Ggt1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HG575.7e-18462.72glutathione hydrolase 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111463200... [more]
A0A6J1HI635.7e-18462.72glutathione hydrolase 1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111463200... [more]
A0A6J1KR703.7e-18362.20glutathione hydrolase 1 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111496901 P... [more]
A0A6J1KLV63.7e-18362.20glutathione hydrolase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496901 P... [more]
A0A6J1KR643.7e-18362.20glutathione hydrolase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496901 P... [more]
Match NameE-valueIdentityDescription
XP_038883429.15.7e-18663.84glutathione hydrolase 1-like isoform X2 [Benincasa hispida][more]
XP_038883430.15.7e-18663.84glutathione hydrolase 1-like isoform X3 [Benincasa hispida][more]
XP_038883431.15.7e-18663.84glutathione hydrolase 1-like isoform X4 [Benincasa hispida] >XP_038883432.1 glut... [more]
XP_038883428.15.7e-18663.84glutathione hydrolase 1-like isoform X1 [Benincasa hispida][more]
KAG6594851.16.9e-18462.72Glutathione hydrolase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT4G39650.12.2e-15152.22gamma-glutamyl transpeptidase 2 [more]
AT4G39640.12.2e-15151.81gamma-glutamyl transpeptidase 1 [more]
AT4G39640.22.2e-15151.81gamma-glutamyl transpeptidase 1 [more]
AT4G29210.11.1e-13146.92gamma-glutamyl transpeptidase 4 [more]
AT4G29210.21.5e-10448.26gamma-glutamyl transpeptidase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR01210GGTRANSPTASEcoord: 143..162
score: 43.86
coord: 424..439
score: 65.06
coord: 125..143
score: 52.15
coord: 276..295
score: 43.41
coord: 373..391
score: 55.98
coord: 448..465
score: 44.7
coord: 246..262
score: 35.83
coord: 57..82
score: 60.49
NoneNo IPR availablePFAMPF01019G_glu_transpeptcoord: 50..553
e-value: 4.0E-152
score: 507.6
NoneNo IPR availablePANTHERPTHR11686:SF34GLUTATHIONE HYDROLASE 1-RELATEDcoord: 8..556
IPR043137Gamma-glutamyltranspeptidase, small subunitGENE3D3.60.20.40coord: 368..558
e-value: 3.5E-55
score: 188.8
IPR043138Gamma-glutamyltranspeptidase, large subunit, C-terminal domainGENE3D1.10.246.130coord: 263..365
e-value: 2.1E-28
score: 100.7
IPR000101Gamma-glutamyltranspeptidaseTIGRFAMTIGR00066TIGR00066coord: 36..550
e-value: 5.2E-114
score: 379.5
IPR000101Gamma-glutamyltranspeptidasePANTHERPTHR11686GAMMA GLUTAMYL TRANSPEPTIDASEcoord: 8..556
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 28..558

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0005633.1PI0005633.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006751 glutathione catabolic process
molecular_function GO:0036374 glutathione hydrolase activity