PI0005288 (gene) Melon (PI 482460) v1

Overview
NamePI0005288
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionPectinesterase
Locationchr01: 25060578 .. 25063820 (-)
RNA-Seq ExpressionPI0005288
SyntenyPI0005288
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACACCCATATCCAACTCTCCCCCTTTCTTCTTCTTCTTCTCCTCCTCTGTTTCAGACATGTTCGGCCTCAAAAACAGAGCTCATATCCCTTCAAATCCCACCTCCATTTCCACCATTTCCACTTTCTTCTTCATTTTCTTTCTCTTCTCCTTCAAACCCATTTCTTCAAATCCCCAAATCCACCAAACCGCTCGATCCCTTTGCAATGGCACTCTGTTCCCAAACCTCTGTTTTTCCAAACTCTCTTCTTTTCCCCAGTTGGCTTCCCTCTCCCCGGAAAAGCTCGCCGGCAGTGCCCTCAACTTCACTACCCGCGAGGTCCTCCTTGCCTACTCCAATTGCACCAACCTCAAGACTCACCTTTACCGTGGCCTGAATCCCACCGACCGCCACGCCCTCGACGACTGTCTTGAGCTTCTCGACGACAGCATTGCGGAGCTCAAAGCTTCGATCTTCGATCTGGCGCCGTCGCAGTCCGCCGCTCGTCACTCCCATGATTTGTTGACATTGGTCAGTGCCGCCATGACCAACCACCGAACTTGTGTTGATGGGTTTTATAATAGTTCGGGGACTGTGAGGAGCAGGGTGGAGCTTTATTTGGGGAAAATTGGACAGCATTTAAGTATCGATTTGGCGATGTTGAAGAAAATCCCTGGAGTTAATAGGGCTACGGGGGTAGAGGAGGTGTTGCCGGAGTATGGGGCAGTGAAAGGTGGGTTTCCGAAGTGGGTTTCGGTTAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAGACGAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTCACGACTGTGGGTGAGGCCGTCGCCGCCGCTCCAAATTCGAGCACCAACAGGTTTTGTTGAACTGAACTAAACTCATAATCATAAGATTTAAAAAATGGTAATTAAGAGAAAAAGTGGATTTGTTATGGGACAGGTTTGTAATATACATAAAAGCAGGGGCATATTTTGAGAACGTGGAAATAGAAAGGGCAAAGTCAAACTTGATGTTCGTCGGCGATGGCATCGGAAAGACGCTGATAAAGGCCAACCGGAATGTCGTCGACGGCTGGACTACTTTCCGATCAGCCACCGTTGGTCAGTTCCCTTTCTTTCTTTATTTATTTTTTTAACTCTTTTTAAAATAAATAAATAAGAAATACAAATATTAATTACTATTATTAAATTTATGAAACCACCTGACTATGATCAGATCTTGGCTCTCATATGATTTTATTTAAGTTGCTACTTTAATTAATTTTTAATAATACAATCTTGGAAAGATTCTTCATAGCAAGAAAATTTTGGATCTCTTTTAATTTTTTATATTTAGAAAACATTTCTTTCAAAAATAAAACTCTCTAGTAACAAAAAAAAAAAAAAAAAAAAAAAAAAAATACAATAACCACCCGCTAGTACAATTAATATTTGTTTTAGAAAAGTATAATTAGTTTCTACAGCGTTAATTTTTAAAAATAAAACTAAATGGTGGATGTCAAATAAACTAATAATTAAACGAAAATGAAAAAGTTTAGTGAAAAGTAACCTTTTGTTATTTTGAATTTAGTTTCTATTTAATTTAAAAATTTTAAAATGTTACACATTTATTTTAAATTTTAATTTTGATTTCAGTTTAATACTTTAAGTATTACAATACTACATCATTATTTTTATAATTTAAGTTTTGTTTCAACTTAAACTAGTTTTCAAAATTTTACATTTTTAATATAATTTTCATTTAAATATTAACTTACAAACATTAAATATAAATGATTATTAATTAATTTAAAGTAGTTATAAAGTAAAGTTTTTAATTTAAGTTTTTTTTAAAAAAAGAATGGTAATAGGGTGTAGGGCGTAGAGTAGAGTGGGGTCCAGAGGTTACAAGAAGGCGACGAAAAATGATGAAAAGGCGTGTCGGTTGGATAACATTGCCATTTACTTGGACAAAACGGTGTTCCTTTTTCTTTATTTTCCTTTTTAAATTAATCAATTTCTTTTTACTATAAAATCCACAAACTCAATACATTTAAAACTCAAACTTGAAAATGTACAAATTTATGCCCACTCATTTCTAAACCTTATAAATTTAATCATAACAAATCTATTTTTGTTTTCTCCATGAATTAAACAATGCAACTAACACATTTTAGTACAATTATCATATCTTTATTATTTATGTTTTCTTCAATATTTCTTTATTGGTTAATTTGATATCAAATATATTCACCCTTGTTTGATATTATAGTTTGGAAAGAGAAAAAAATAGAATTAAATTTCTAAAATTTATTCACTATTAGCAATAACGCAAGTTTTGTTTATTATGTAGCTGTGGTTGGCACTGGGTTTATAGCAAAAGGCATAACATTTGAAAATTATGCGGGTCCAAGCAAGCACCAAGCTGTAGCCTTACGAAGCAACTCTGACTTCTCTGCATTTTACCAGTGCAGCTTCATTGGCTATCAAGACACTCTCTACGTTCACTCCCTCCGTCAATTCTATCGCGAATGCGATATCTACGGTACCATCGACTTCATTTTTGGCAACGCTGCTGTTGTTTTCCAAAACTGCAACCTTTATGCTCGAAAACCCAACTCAAATCAACGCAACATATTCACTGCTCAAGGTCGCGAGGACCCCAATCAAAATACTGGAATCTCAATCCTCAATTGTAAGGTTGAAGCTGCCTCTGACCTAATCCCGGTTCTTTCCTCGTTTAGAACTTACTTGGGTCGACCTTGGAAGTTGCACTCGAGGACAGTTTTTCTTCGATCGTTTATTGGGCAATTGGTTGAACCAGTTGGATGGCTTGAGTGGAATGGGACATTTGCATTGGATACTTTGTATTATGGGGAGTATTTGAACAGGGGGCCTGGTTCGAATACAACCAGACGAGTTACTTGGCCAGGTTATCGAGTTATTACTAACGCAACGGAAGCGAGTCAGTTTACGGTCGAAGGTTTTATACAAGGTAGCTCGTGGTTGAACTCGACTGGGATCCCTTTCTTCTCCGGTTTGGCTTCACCGTGAATCGGAAGGTTAAAAATTTAGAGTTATCTTTGTGTTTTTATTTTCATTTTTTTGTAAGGACTAAATGTTTTTGATGTTGAACTAGGGAGAGAGTAATGGCTGATCCATGGTCCACAAAAGGACTTCATGTGATCATATGAAATCATTGGTAGTTCCCATTTTAATCAAATATTATATACCTAAAATTTGAAGTAGCA

mRNA sequence

AACACCCATATCCAACTCTCCCCCTTTCTTCTTCTTCTTCTCCTCCTCTGTTTCAGACATGTTCGGCCTCAAAAACAGAGCTCATATCCCTTCAAATCCCACCTCCATTTCCACCATTTCCACTTTCTTCTTCATTTTCTTTCTCTTCTCCTTCAAACCCATTTCTTCAAATCCCCAAATCCACCAAACCGCTCGATCCCTTTGCAATGGCACTCTGTTCCCAAACCTCTGTTTTTCCAAACTCTCTTCTTTTCCCCAGTTGGCTTCCCTCTCCCCGGAAAAGCTCGCCGGCAGTGCCCTCAACTTCACTACCCGCGAGGTCCTCCTTGCCTACTCCAATTGCACCAACCTCAAGACTCACCTTTACCGTGGCCTGAATCCCACCGACCGCCACGCCCTCGACGACTGTCTTGAGCTTCTCGACGACAGCATTGCGGAGCTCAAAGCTTCGATCTTCGATCTGGCGCCGTCGCAGTCCGCCGCTCGTCACTCCCATGATTTGTTGACATTGGTCAGTGCCGCCATGACCAACCACCGAACTTGTGTTGATGGGTTTTATAATAGTTCGGGGACTGTGAGGAGCAGGGTGGAGCTTTATTTGGGGAAAATTGGACAGCATTTAAGTATCGATTTGGCGATGTTGAAGAAAATCCCTGGAGTTAATAGGGCTACGGGGGTAGAGGAGGTGTTGCCGGAGTATGGGGCAGTGAAAGGTGGGTTTCCGAAGTGGGTTTCGGTTAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAGACGAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTCACGACTGTGGGTGAGGCCGTCGCCGCCGCTCCAAATTCGAGCACCAACAGGTTTGTAATATACATAAAAGCAGGGGCATATTTTGAGAACGTGGAAATAGAAAGGGCAAAGTCAAACTTGATGTTCGTCGGCGATGGCATCGGAAAGACGCTGATAAAGGCCAACCGGAATGTCGTCGACGGCTGGACTACTTTCCGATCAGCCACCGTTGCTGTGGTTGGCACTGGGTTTATAGCAAAAGGCATAACATTTGAAAATTATGCGGGTCCAAGCAAGCACCAAGCTGTAGCCTTACGAAGCAACTCTGACTTCTCTGCATTTTACCAGTGCAGCTTCATTGGCTATCAAGACACTCTCTACGTTCACTCCCTCCGTCAATTCTATCGCGAATGCGATATCTACGGTACCATCGACTTCATTTTTGGCAACGCTGCTGTTGTTTTCCAAAACTGCAACCTTTATGCTCGAAAACCCAACTCAAATCAACGCAACATATTCACTGCTCAAGGTCGCGAGGACCCCAATCAAAATACTGGAATCTCAATCCTCAATTGTAAGGTTGAAGCTGCCTCTGACCTAATCCCGGTTCTTTCCTCGTTTAGAACTTACTTGGGTCGACCTTGGAAGTTGCACTCGAGGACAGTTTTTCTTCGATCGTTTATTGGGCAATTGGTTGAACCAGTTGGATGGCTTGAGTGGAATGGGACATTTGCATTGGATACTTTGTATTATGGGGAGTATTTGAACAGGGGGCCTGGTTCGAATACAACCAGACGAGTTACTTGGCCAGGTTATCGAGTTATTACTAACGCAACGGAAGCGAGTCAGTTTACGGTCGAAGGTTTTATACAAGGTAGCTCGTGGTTGAACTCGACTGGGATCCCTTTCTTCTCCGGTTTGGCTTCACCGTGAATCGGAAGGTTAAAAATTTAGAGTTATCTTTGTGTTTTTATTTTCATTTTTTTGTAAGGACTAAATGTTTTTGATGTTGAACTAGGGAGAGAGTAATGGCTGATCCATGGTCCACAAAAGGACTTCATGTGATCATATGAAATCATTGGTAGTTCCCATTTTAATCAAATATTATATACCTAAAATTTGAAGTAGCA

Coding sequence (CDS)

ATGTTCGGCCTCAAAAACAGAGCTCATATCCCTTCAAATCCCACCTCCATTTCCACCATTTCCACTTTCTTCTTCATTTTCTTTCTCTTCTCCTTCAAACCCATTTCTTCAAATCCCCAAATCCACCAAACCGCTCGATCCCTTTGCAATGGCACTCTGTTCCCAAACCTCTGTTTTTCCAAACTCTCTTCTTTTCCCCAGTTGGCTTCCCTCTCCCCGGAAAAGCTCGCCGGCAGTGCCCTCAACTTCACTACCCGCGAGGTCCTCCTTGCCTACTCCAATTGCACCAACCTCAAGACTCACCTTTACCGTGGCCTGAATCCCACCGACCGCCACGCCCTCGACGACTGTCTTGAGCTTCTCGACGACAGCATTGCGGAGCTCAAAGCTTCGATCTTCGATCTGGCGCCGTCGCAGTCCGCCGCTCGTCACTCCCATGATTTGTTGACATTGGTCAGTGCCGCCATGACCAACCACCGAACTTGTGTTGATGGGTTTTATAATAGTTCGGGGACTGTGAGGAGCAGGGTGGAGCTTTATTTGGGGAAAATTGGACAGCATTTAAGTATCGATTTGGCGATGTTGAAGAAAATCCCTGGAGTTAATAGGGCTACGGGGGTAGAGGAGGTGTTGCCGGAGTATGGGGCAGTGAAAGGTGGGTTTCCGAAGTGGGTTTCGGTTAAGGATCGGAGATTGTTGCAGGCGGCGGTGAATGAGACGAAGTTCAATTTGGTGGTGGCGAAGGATGGCTCCGGGAATTTCACGACTGTGGGTGAGGCCGTCGCCGCCGCTCCAAATTCGAGCACCAACAGGTTTGTAATATACATAAAAGCAGGGGCATATTTTGAGAACGTGGAAATAGAAAGGGCAAAGTCAAACTTGATGTTCGTCGGCGATGGCATCGGAAAGACGCTGATAAAGGCCAACCGGAATGTCGTCGACGGCTGGACTACTTTCCGATCAGCCACCGTTGCTGTGGTTGGCACTGGGTTTATAGCAAAAGGCATAACATTTGAAAATTATGCGGGTCCAAGCAAGCACCAAGCTGTAGCCTTACGAAGCAACTCTGACTTCTCTGCATTTTACCAGTGCAGCTTCATTGGCTATCAAGACACTCTCTACGTTCACTCCCTCCGTCAATTCTATCGCGAATGCGATATCTACGGTACCATCGACTTCATTTTTGGCAACGCTGCTGTTGTTTTCCAAAACTGCAACCTTTATGCTCGAAAACCCAACTCAAATCAACGCAACATATTCACTGCTCAAGGTCGCGAGGACCCCAATCAAAATACTGGAATCTCAATCCTCAATTGTAAGGTTGAAGCTGCCTCTGACCTAATCCCGGTTCTTTCCTCGTTTAGAACTTACTTGGGTCGACCTTGGAAGTTGCACTCGAGGACAGTTTTTCTTCGATCGTTTATTGGGCAATTGGTTGAACCAGTTGGATGGCTTGAGTGGAATGGGACATTTGCATTGGATACTTTGTATTATGGGGAGTATTTGAACAGGGGGCCTGGTTCGAATACAACCAGACGAGTTACTTGGCCAGGTTATCGAGTTATTACTAACGCAACGGAAGCGAGTCAGTTTACGGTCGAAGGTTTTATACAAGGTAGCTCGTGGTTGAACTCGACTGGGATCCCTTTCTTCTCCGGTTTGGCTTCACCGTGA

Protein sequence

MFGLKNRAHIPSNPTSISTISTFFFIFFLFSFKPISSNPQIHQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTTREVLLAYSNCTNLKTHLYRGLNPTDRHALDDCLELLDDSIAELKASIFDLAPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRATGVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTNRFVIYIKAGAYFENVEIERAKSNLMFVGDGIGKTLIKANRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTRRVTWPGYRVITNATEASQFTVEGFIQGSSWLNSTGIPFFSGLASP
Homology
BLAST of PI0005288 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 2.1e-153
Identity = 278/488 (56.97%), Postives = 351/488 (71.93%), Query Frame = 0

Query: 75  KLAGSALNFTTREVLLAYSNCTNLKTHLYRGLNPTDRHALDDCLELLDDSIAELKASIFD 134
           +L  +ALN T   V L+ SN ++L   L   L+  D  A DDCLELLDD++ +L  +I  
Sbjct: 39  ELVVTALNQTISNVNLSSSNFSDLLQRLGSNLSHRDLCAFDDCLELLDDTVFDLTTAISK 98

Query: 135 LAPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGT------------VRSRVELYLG 194
           L   +S +   H++  L+SAAMTN RTC+DGF +S               V   ++  L 
Sbjct: 99  L---RSHSPELHNVKMLLSAAMTNTRTCLDGFASSDNDENLNNNDNKTYGVAESLKESLF 158

Query: 195 KIGQHLSIDLAMLKKIPGVNRATGVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETKF 254
            I  H+S  LAML+ IPG       E+V         GFP WVS  DR LLQ  V+ETK 
Sbjct: 159 NISSHVSDSLAMLENIPGHIPGKVKEDV---------GFPMWVSGSDRNLLQDPVDETKV 218

Query: 255 NLVVAKDGSGNFTTVGEAVAAAPNSSTNRFVIYIKAGAYFENVEIERAKSNLMFVGDGIG 314
           NLVVA++G+GN+TT+GEA++AAPNSS  RFVIYIK G YFEN+EI R K+ +MF+GDGIG
Sbjct: 219 NLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIG 278

Query: 315 KTLIKANRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFY 374
           +T+IKANR+  DGWT F SATV V G+GFIAK ++F NYAGP KHQAVALRS+SD SA+Y
Sbjct: 279 RTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSSDLSAYY 338

Query: 375 QCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTA 434
           +CSF  YQDT+YVHS +QFYRECDIYGT+DFIFG+A+VVFQNC+LYAR+PN NQ+ I+TA
Sbjct: 339 RCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQKIIYTA 398

Query: 435 QGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVEPV 494
           QGRE+  + TGISI++ ++ AA DLIPV ++F+ YLGRPW+L+SRTV ++SFI  LV+P 
Sbjct: 399 QGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFIDDLVDPA 458

Query: 495 GWLEWNGTFALDTLYYGEYLNRGPGSNTTRRVTWPGYRVITNATEASQFTVEGFIQGSSW 551
           GWL+W   FAL+TLYYGEY+N GPGSN T RV WPG++ I    EASQF+V  FI G+ W
Sbjct: 459 GWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGNKW 514

BLAST of PI0005288 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 3.6e-145
Identity = 267/481 (55.51%), Postives = 337/481 (70.06%), Query Frame = 0

Query: 81  LNFTTREVLLAYSNCTNLKTHLYRGLNPTDRHALDDCLELLDDSIAELKASIFDLAPSQS 140
           LN T  +V LA SN ++L+T L+  L   +R A +DCL LLDD+I++L+ ++ DL   +S
Sbjct: 61  LNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSDL---RS 120

Query: 141 AARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELYLGK--------IGQHLSIDL 200
           ++   +D+  L++  MT   TC+DGF  S     + +   L +        I  +LS  L
Sbjct: 121 SSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISNNLSNSL 180

Query: 201 AMLKKIPGVNRATGVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSG 260
            ML+ I     +    EV  EY       P W+S  D+RLL+A V ET +NL VA DG+G
Sbjct: 181 HMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSVAIDGTG 240

Query: 261 NFTTVGEAVAAAPNSSTNRFVIYIKAGAYFENVEIERAKSNLMFVGDGIGKTLIKANRNV 320
           NFTT+ +AV AAPN S  RF+IYIK G YFENVE+ + K+ +MF+GDGIGKT+IKANR+ 
Sbjct: 241 NFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANRSR 300

Query: 321 VDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDT 380
           +DGW+TF++ TV V G G+IAK I+F N AGP+K QAVA RS SD SAFY+C F GYQDT
Sbjct: 301 IDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEFDGYQDT 360

Query: 381 LYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNT 440
           LYVHS +QFYRECDIYGTIDFIFGNAAVVFQN +LYARKPN   +  FTAQ R   +Q T
Sbjct: 361 LYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRNQSDQPT 420

Query: 441 GISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVEPVGWLEWNGTFA 500
           GISILNC++ AA DLIPV  +F+ YLGRPW+ +SRTV ++SFI  L+ P GWLE    FA
Sbjct: 421 GISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEGKKDFA 480

Query: 501 LDTLYYGEYLNRGPGSNTTRRVTWPGYRVITNATEASQFTVEGFIQGSSWLNSTGIPFFS 554
           L+TLYYGEY+N GPG+N  +RVTWPG+R I N TEA+QFTV  FI GS+WLNSTGIPF  
Sbjct: 481 LETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFSL 531

BLAST of PI0005288 vs. ExPASy Swiss-Prot
Match: P85076 (Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 1.2e-143
Identity = 247/319 (77.43%), Postives = 274/319 (85.89%), Query Frame = 0

Query: 236 AVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTNRFVIYIKAGAYFENVEIERAKSNLM 295
           AV +   ++VVAKDGSGNFTTVG AVAAA +SST RFVIYIK GAYFE V++++ K+NLM
Sbjct: 2   AVTDIVPDVVVAKDGSGNFTTVGAAVAAAKDSSTARFVIYIKEGAYFEYVDVDKKKTNLM 61

Query: 296 FVGDGIGKTLIKANRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSN 355
           F+GDGIGKT IK NR+VVDGWTTFRS+TVAVVGTGFIA+GI+FENYAGPSKHQAVALRS 
Sbjct: 62  FIGDGIGKTWIKGNRSVVDGWTTFRSSTVAVVGTGFIARGISFENYAGPSKHQAVALRSG 121

Query: 356 SDFSAFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSN 415
           +DFSAFYQCSF+GYQDTLYVHSLRQFY ECD+YGTIDFIFGNAA V Q CNLYARKPN N
Sbjct: 122 ADFSAFYQCSFVGYQDTLYVHSLRQFYSECDVYGTIDFIFGNAAAVLQKCNLYARKPNEN 181

Query: 416 QRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFI 475
           Q+NIFTAQGR+DPNQNTGISILNCKV AA+DLIPVLSSF+TYLGRPWK +SRTVFL S +
Sbjct: 182 QKNIFTAQGRDDPNQNTGISILNCKVAAAADLIPVLSSFKTYLGRPWKEYSRTVFLLSQM 241

Query: 476 GQLVEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTRRVTWPGYRVITNATEASQFTVEG 535
             L++P GWLEW+G FAL TLYY EY N GPGSNTT RVTWPGY V TN TE  QFTV  
Sbjct: 242 ESLIDPAGWLEWSGDFALTTLYYREYKNTGPGSNTTARVTWPGYAVTTNETEVIQFTVGN 301

Query: 536 FIQGSSWLNSTGIPFFSGL 555
           FIQGS WL S  IP +  L
Sbjct: 302 FIQGSQWLTSYNIPVYLNL 320

BLAST of PI0005288 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 499.6 bits (1285), Expect = 4.6e-140
Identity = 271/530 (51.13%), Postives = 348/530 (65.66%), Query Frame = 0

Query: 42  HQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTTREVLLAYSNCTNLKTH 101
           H   +S+C+ TL+P LCFS +++       S +++  ++LN TT+ V   Y     L   
Sbjct: 59  HAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAK 118

Query: 102 LYRGLNPTDRHALDDCLELLDDSIAELKASIFDL--APSQSAAR-HSHDLLTLVSAAMTN 161
             +GL P +  AL DCLE +D+++ EL  ++ DL   P Q + R H+ DL TL+S+A+TN
Sbjct: 119 -RKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITN 178

Query: 162 HRTCVDGFYNSSGTVRSRVELYLGKIG-QHL-SIDLAMLKKIPGV--------------- 221
             TC+DGF       + R  L  G++  +H+ S  LAM+K +                  
Sbjct: 179 QGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFT 238

Query: 222 -NRATGVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEA 281
            N    ++EV  +  +   G+PKW+SV DRRLLQ +    K +  VA DGSG+FTTV  A
Sbjct: 239 NNNNRKLKEVTGDLDS--DGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAA 298

Query: 282 VAAAPNSSTNRFVIYIKAGAYFENVEIERAKSNLMFVGDGIGKTLIKANRNVVDGWTTFR 341
           VAAAP  S  RFVI+IKAG Y ENVE+ + K+N+MF+GDG GKT+I  +RNVVDG TTF 
Sbjct: 299 VAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFH 358

Query: 342 SATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQ 401
           SATVA VG  F+A+ ITF+N AGPSKHQAVALR  SDFSAFYQC    YQDTLYVHS RQ
Sbjct: 359 SATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQ 418

Query: 402 FYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCK 461
           F+ +C I GT+DFIFGNAA V Q+C++ AR+PNS Q+N+ TAQGR DPNQNTGI I NC+
Sbjct: 419 FFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCR 478

Query: 462 VEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVEPVGWLEWNGTFALDTLYYGE 521
           +   SDL+ V  +F TYLGRPWK +SRTV ++S I  ++ P GW EW+G+FALDTL Y E
Sbjct: 479 IGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYRE 538

Query: 522 YLNRGPGSNTTRRVTWPGYRVITNATEASQFTVEGFIQGSSWLNSTGIPF 551
           YLNRG G+ T  RV W GY+VIT+ TEA  FT   FI G  WL STG PF
Sbjct: 539 YLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

BLAST of PI0005288 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 1.9e-138
Identity = 266/530 (50.19%), Postives = 347/530 (65.47%), Query Frame = 0

Query: 36  SSNPQIHQTARSLCNGTLFPNLCFSKLSSFPQLAS--LSPEKLAGSALNFTTREVLLAYS 95
           + N   H   +S C+ T +P+LCFS +++ P+ +    S + +   +LN TT  V   Y 
Sbjct: 60  NGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYF 119

Query: 96  NCTNLKTHLYRGLNPTDRHALDDCLELLDDSIAELKASIFDLAP---SQSAARHSHDLLT 155
               L       L   ++ AL DCLE +D+++ EL  ++ DL      +S ++H+ DL T
Sbjct: 120 GIQKLLKR--TNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKT 179

Query: 156 LVSAAMTNHRTCVDGFYNSSGTVRSRVELYLGK--IGQHLSIDLAMLKKIPG----VNRA 215
           L+SAAMTN  TC+DGF +       R  L  G+  + +  S  LAM+K +      + R 
Sbjct: 180 LMSAAMTNQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRT 239

Query: 216 TGVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAA 275
           +   +++ E   V  G+P W+S  DRRLLQ+  +    N+VVA DGSGNF TV  +VAAA
Sbjct: 240 SNNRKLIEETSTV-DGWPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAA 299

Query: 276 PNSSTNRFVIYIKAGAYFENVEIERAKSNLMFVGDGIGKTLIKANRNVVDGWTTFRSATV 335
           P   T R++I IKAG Y ENVE+ +   N+MF+GDG  +T+I  +RNVVDG TTF+SATV
Sbjct: 300 PQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATV 359

Query: 336 AVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRE 395
           AVVG GF+A+ ITF+N AGPSKHQAVALR  +D SAFY C  + YQDTLYVHS RQF+  
Sbjct: 360 AVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVN 419

Query: 396 CDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAA 455
           C I GT+DFIFGNAA V QNC+++ARKPNS Q+N+ TAQGR DPNQNTGI I   ++ A 
Sbjct: 420 CLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGAT 479

Query: 456 SDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVEPVGWLEWNGTFALDTLYYGEYLNR 515
           SDL PV  SF TYLGRPWK +SRTV ++S I  ++ P GW EW+G FAL+TL+YGE+ N 
Sbjct: 480 SDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNA 539

Query: 516 GPGSNTTRRVTWPGYRVITNATEASQFTVEGFIQGSSWLNSTGIPFFSGL 555
           G G+ T+ RV W G+RVIT+ATEA  FT   FI GSSWL STG PF  GL
Sbjct: 540 GAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of PI0005288 vs. ExPASy TrEMBL
Match: A0A1S3BSU3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1)

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 539/557 (96.77%), Postives = 543/557 (97.49%), Query Frame = 0

Query: 1   MFGLKNRAHIPSNPTSISTISTFFFIFFLFSFKPISSNPQIHQTARSLCNGTLFPNLCFS 60
           M+GL+NRA I SNPTSISTISTFFFIFFLFS KPISSNPQIHQTARSLCNGTLFPNLCFS
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFS 60

Query: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYSNCTNLKTHLYRGLNPTDRHALDDCLEL 120
           KLSSFPQLASLSPEKLAGSALNFTTREVLLAY+NCTNLKTHLY GLNPTDRHALDDCLEL
Sbjct: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLEL 120

Query: 121 LDDSIAELKASIFDLAPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180
           LDDSIAELKASI DL PSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY
Sbjct: 121 LDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180

Query: 181 LGKIGQHLSIDLAMLKKIPGVNRATGVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET 240
           LGKIGQHLSIDLAMLKKIPGVNRAT VEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET
Sbjct: 181 LGKIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET 240

Query: 241 KFNLVVAKDGSGNFTTVGEAVAAAPNSSTNRFVIYIKAGAYFENVEIERAKSNLMFVGDG 300
           KFNLVVAKDGSGNFTTV EAVAAAPNSST RFVIYIKAGAYFENVEI RAKSNLMFVGDG
Sbjct: 241 KFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGDG 300

Query: 301 IGKTLIKANRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360
           IGKTLIK +RNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA
Sbjct: 301 IGKTLIKGDRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360

Query: 361 FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420
           FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF
Sbjct: 361 FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420

Query: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE 480
           TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE
Sbjct: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE 480

Query: 481 PVGWLEWNGTFALDTLYYGEYLNRGPGSNTTRRVTWPGYRVITNATEASQFTVEGFIQGS 540
           PVGWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITNATEASQFTVE FIQGS
Sbjct: 481 PVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQGS 540

Query: 541 SWLNSTGIPFFSGLASP 558
           SWLNSTGIPFFSGL SP
Sbjct: 541 SWLNSTGIPFFSGLTSP 557

BLAST of PI0005288 vs. ExPASy TrEMBL
Match: A0A5D3D1W6 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001300 PE=3 SV=1)

HSP 1 Score: 1081.6 bits (2796), Expect = 0.0e+00
Identity = 538/557 (96.59%), Postives = 543/557 (97.49%), Query Frame = 0

Query: 1   MFGLKNRAHIPSNPTSISTISTFFFIFFLFSFKPISSNPQIHQTARSLCNGTLFPNLCFS 60
           M+GL+NRA I SNPTSISTISTFFFIFFLFS KPISSNPQIHQTARSLCNGTLFPNLCFS
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFS 60

Query: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYSNCTNLKTHLYRGLNPTDRHALDDCLEL 120
           KLSSFPQLASLSPEKLAGSALNFTTREVLLAY+NCTNLKTHLY GLNPTDRHALDDCLEL
Sbjct: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLEL 120

Query: 121 LDDSIAELKASIFDLAPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180
           LDDSIAELKASI DL PSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY
Sbjct: 121 LDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180

Query: 181 LGKIGQHLSIDLAMLKKIPGVNRATGVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET 240
           LGKIGQHLSIDLAMLKKIPGVNRAT VEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET
Sbjct: 181 LGKIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET 240

Query: 241 KFNLVVAKDGSGNFTTVGEAVAAAPNSSTNRFVIYIKAGAYFENVEIERAKSNLMFVGDG 300
           KFNLVVAKDGSGNFTTV EAVAAAPNSST RFVIYIKAGAYFENVEI RAKSNLMFVGDG
Sbjct: 241 KFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGDG 300

Query: 301 IGKTLIKANRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360
           IGKTLIK +RNVVDGWTTFRSATVAV+GTGFIAKGITFENYAGPSKHQAVALRSNSDFSA
Sbjct: 301 IGKTLIKGDRNVVDGWTTFRSATVAVIGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360

Query: 361 FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420
           FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF
Sbjct: 361 FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420

Query: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE 480
           TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE
Sbjct: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE 480

Query: 481 PVGWLEWNGTFALDTLYYGEYLNRGPGSNTTRRVTWPGYRVITNATEASQFTVEGFIQGS 540
           PVGWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITNATEASQFTVE FIQGS
Sbjct: 481 PVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQGS 540

Query: 541 SWLNSTGIPFFSGLASP 558
           SWLNSTGIPFFSGL SP
Sbjct: 541 SWLNSTGIPFFSGLTSP 557

BLAST of PI0005288 vs. ExPASy TrEMBL
Match: A0A6J1JU83 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487942 PE=3 SV=1)

HSP 1 Score: 1005.0 bits (2597), Expect = 1.2e-289
Identity = 500/556 (89.93%), Postives = 519/556 (93.35%), Query Frame = 0

Query: 1   MFGLKNRAHIPSNPT-SISTISTFFFIFFLFSFKPISSNPQIHQTARSLCNGTLFPNLCF 60
           M+GL++RA   SNPT SIS I T F + FLFSFKPISSNPQ HQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTLFSVLFLFSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTTREVLLAYSNCTNLKTHLYRGLNPTDRHALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFT  EV  AY+NC+ LKT  Y GLNPTDR ALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDDSIAELKASIFDLAPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVEL 180
           LLD SIAEL+ SI+DL+PSQSAARHSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIGQHLSIDLAMLKKIPGVNRATGVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 240
           YLGKI QHLSIDLAMLKKIPGV RA   EEVLPEYGAV+GGFP WVSVKDRRLLQAAVNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQAAVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTNRFVIYIKAGAYFENVEIERAKSNLMFVGD 300
           TKFNLVVAKDGSGNFTTVGEAVAAAPNSST RFVIYIKAGAYFENVEIER K+NLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKANRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFS 360
           GIGKTLIKA+RNVVDGWTTFRSATVA VG GFIAKGITFEN+AGPSKHQAVA RSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVAAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKL+SRT+FL+SFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTIFLKSFIGQLV 480

Query: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTRRVTWPGYRVITNATEASQFTVEGFIQG 540
           EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITN+TEASQFTVE FIQG
Sbjct: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SSWLNSTGIPFFSGLA 556
           S WLNST IPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of PI0005288 vs. ExPASy TrEMBL
Match: A0A6J1GNU4 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456156 PE=3 SV=1)

HSP 1 Score: 994.6 bits (2570), Expect = 1.7e-286
Identity = 494/556 (88.85%), Postives = 516/556 (92.81%), Query Frame = 0

Query: 1   MFGLKNRAHIPSNPT-SISTISTFFFIFFLFSFKPISSNPQIHQTARSLCNGTLFPNLCF 60
           M+GL++RA   SNPT SIS I TFF + FL SFKPISSNPQ HQTARSLCNGTLFPNLCF
Sbjct: 1   MYGLRSRAQSSSNPTASISAIFTFFSVLFLCSFKPISSNPQTHQTARSLCNGTLFPNLCF 60

Query: 61  SKLSSFPQLASLSPEKLAGSALNFTTREVLLAYSNCTNLKTHLYRGLNPTDRHALDDCLE 120
           SKLSSFPQLASLSPEKLAGSALNFT  EV  AY+NC+ LKT  Y GLNPTDR ALDDCLE
Sbjct: 61  SKLSSFPQLASLSPEKLAGSALNFTNDEVHGAYANCSKLKTLFYGGLNPTDRRALDDCLE 120

Query: 121 LLDDSIAELKASIFDLAPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVEL 180
           LLD SIAEL+ SI+DL+PSQSAARHSHDLLTLVSAAMTNHRTC+DGFYNSSGTVR RVEL
Sbjct: 121 LLDGSIAELQTSIYDLSPSQSAARHSHDLLTLVSAAMTNHRTCLDGFYNSSGTVRKRVEL 180

Query: 181 YLGKIGQHLSIDLAMLKKIPGVNRATGVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNE 240
           YLGKI QHLSIDLAMLKKIPGV RA   EEVLPEYGAV+GGFP WVSVKDRRLLQA+VNE
Sbjct: 181 YLGKIEQHLSIDLAMLKKIPGVKRAATAEEVLPEYGAVRGGFPMWVSVKDRRLLQASVNE 240

Query: 241 TKFNLVVAKDGSGNFTTVGEAVAAAPNSSTNRFVIYIKAGAYFENVEIERAKSNLMFVGD 300
           TKFNLVVAKDGSGNFTTVG AVAAAPNSST RFVIYIKAGAYFENVEIER K+NLMFVGD
Sbjct: 241 TKFNLVVAKDGSGNFTTVGAAVAAAPNSSTTRFVIYIKAGAYFENVEIERKKTNLMFVGD 300

Query: 301 GIGKTLIKANRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFS 360
           GIGKTLIKA+RNVVDGWTTFRSATV  VG GFIAKGITFEN+AGPSKHQAVA RSNSDFS
Sbjct: 301 GIGKTLIKADRNVVDGWTTFRSATVVAVGNGFIAKGITFENFAGPSKHQAVAFRSNSDFS 360

Query: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNI 420
           AFYQCSFIGYQDTLYVHSLRQFYRECD+YGTIDFIFGNAAV+FQNCNLYARKPNSNQRNI
Sbjct: 361 AFYQCSFIGYQDTLYVHSLRQFYRECDVYGTIDFIFGNAAVIFQNCNLYARKPNSNQRNI 420

Query: 421 FTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLV 480
           FTAQGREDPNQNTGISILNCKVEAASDL+PVL SFRTYLGRPWKL+SRTVFL+SFIGQLV
Sbjct: 421 FTAQGREDPNQNTGISILNCKVEAASDLLPVLPSFRTYLGRPWKLYSRTVFLKSFIGQLV 480

Query: 481 EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTRRVTWPGYRVITNATEASQFTVEGFIQG 540
           EPVGWLEW+GTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITN+TEASQFTVE FIQG
Sbjct: 481 EPVGWLEWSGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNSTEASQFTVEAFIQG 540

Query: 541 SSWLNSTGIPFFSGLA 556
           S WLNST IPFFSGLA
Sbjct: 541 SEWLNSTEIPFFSGLA 556

BLAST of PI0005288 vs. ExPASy TrEMBL
Match: A0A0A0K5K8 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1)

HSP 1 Score: 993.4 bits (2567), Expect = 3.8e-286
Identity = 493/525 (93.90%), Postives = 506/525 (96.38%), Query Frame = 0

Query: 34  PISSNPQIHQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTTREVLLAYS 93
           P+      HQTARSLCNGTLFP+LCFSKLSSFPQLASLSPEKLAGSALNFT+REVLLAY+
Sbjct: 90  PLPLFQTFHQTARSLCNGTLFPDLCFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYT 149

Query: 94  NCTNLKTHLYRGLNPTDRHALDDCLELLDDSIAELKASIFDLAPSQSAARHSHDLLTLVS 153
           NCTNLKTHLY GLNPTDRHALDDCLELLD SIAELKASIFDLAPSQS A HSHDLLTLVS
Sbjct: 150 NCTNLKTHLYSGLNPTDRHALDDCLELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVS 209

Query: 154 AAMTNHRTCVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRATGV-EEVLP 213
           AAMTNHRTCVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRATGV +E+LP
Sbjct: 210 AAMTNHRTCVDGFYNSSGTVRSRVELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLP 269

Query: 214 EYGAVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTNRF 273
           EYGAVKGGFPKWVSVKDRRLLQAAVNETKFN+VVAKDGSGNFTTV EAVAAAPN+ST RF
Sbjct: 270 EYGAVKGGFPKWVSVKDRRLLQAAVNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRF 329

Query: 274 VIYIKAGAYFENVEIERAKSNLMFVGDGIGKTLIKANRNVVDGWTTFRSATVAVVGTGFI 333
           VIYIKAGAYFENVEI RAKSNLMFVGDGIGKTLIKA+RNVVDGWTTFRSATVAVVGTGFI
Sbjct: 330 VIYIKAGAYFENVEIGRAKSNLMFVGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFI 389

Query: 334 AKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDIYGTID 393
           AKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYR+CD+YGTID
Sbjct: 390 AKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTID 449

Query: 394 FIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLS 453
           FIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLS
Sbjct: 450 FIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLS 509

Query: 454 SFRTYLGRPWKLHSRTVFLRSFIGQLVEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTR 513
           SFRTYLGRPWKL+SRTVFLRSFIGQL+EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTT 
Sbjct: 510 SFRTYLGRPWKLYSRTVFLRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTM 569

Query: 514 RVTWPGYRVITNATEASQFTVEGFIQGSSWLNSTGIPFFSGLASP 558
           RVTWPGYRVITNATEASQFTVE FIQGSSWLNST IPFFSGL  P
Sbjct: 570 RVTWPGYRVITNATEASQFTVERFIQGSSWLNSTEIPFFSGLTPP 614

BLAST of PI0005288 vs. NCBI nr
Match: XP_008451545.2 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 539/557 (96.77%), Postives = 543/557 (97.49%), Query Frame = 0

Query: 1   MFGLKNRAHIPSNPTSISTISTFFFIFFLFSFKPISSNPQIHQTARSLCNGTLFPNLCFS 60
           M+GL+NRA I SNPTSISTISTFFFIFFLFS KPISSNPQIHQTARSLCNGTLFPNLCFS
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFS 60

Query: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYSNCTNLKTHLYRGLNPTDRHALDDCLEL 120
           KLSSFPQLASLSPEKLAGSALNFTTREVLLAY+NCTNLKTHLY GLNPTDRHALDDCLEL
Sbjct: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLEL 120

Query: 121 LDDSIAELKASIFDLAPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180
           LDDSIAELKASI DL PSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY
Sbjct: 121 LDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180

Query: 181 LGKIGQHLSIDLAMLKKIPGVNRATGVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET 240
           LGKIGQHLSIDLAMLKKIPGVNRAT VEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET
Sbjct: 181 LGKIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET 240

Query: 241 KFNLVVAKDGSGNFTTVGEAVAAAPNSSTNRFVIYIKAGAYFENVEIERAKSNLMFVGDG 300
           KFNLVVAKDGSGNFTTV EAVAAAPNSST RFVIYIKAGAYFENVEI RAKSNLMFVGDG
Sbjct: 241 KFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGDG 300

Query: 301 IGKTLIKANRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360
           IGKTLIK +RNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA
Sbjct: 301 IGKTLIKGDRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360

Query: 361 FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420
           FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF
Sbjct: 361 FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420

Query: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE 480
           TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE
Sbjct: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE 480

Query: 481 PVGWLEWNGTFALDTLYYGEYLNRGPGSNTTRRVTWPGYRVITNATEASQFTVEGFIQGS 540
           PVGWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITNATEASQFTVE FIQGS
Sbjct: 481 PVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQGS 540

Query: 541 SWLNSTGIPFFSGLASP 558
           SWLNSTGIPFFSGL SP
Sbjct: 541 SWLNSTGIPFFSGLTSP 557

BLAST of PI0005288 vs. NCBI nr
Match: KAA0046658.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYK18193.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1081.6 bits (2796), Expect = 0.0e+00
Identity = 538/557 (96.59%), Postives = 543/557 (97.49%), Query Frame = 0

Query: 1   MFGLKNRAHIPSNPTSISTISTFFFIFFLFSFKPISSNPQIHQTARSLCNGTLFPNLCFS 60
           M+GL+NRA I SNPTSISTISTFFFIFFLFS KPISSNPQIHQTARSLCNGTLFPNLCFS
Sbjct: 1   MYGLRNRAQISSNPTSISTISTFFFIFFLFSSKPISSNPQIHQTARSLCNGTLFPNLCFS 60

Query: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYSNCTNLKTHLYRGLNPTDRHALDDCLEL 120
           KLSSFPQLASLSPEKLAGSALNFTTREVLLAY+NCTNLKTHLY GLNPTDRHALDDCLEL
Sbjct: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYANCTNLKTHLYCGLNPTDRHALDDCLEL 120

Query: 121 LDDSIAELKASIFDLAPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180
           LDDSIAELKASI DL PSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY
Sbjct: 121 LDDSIAELKASILDLGPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180

Query: 181 LGKIGQHLSIDLAMLKKIPGVNRATGVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET 240
           LGKIGQHLSIDLAMLKKIPGVNRAT VEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET
Sbjct: 181 LGKIGQHLSIDLAMLKKIPGVNRATKVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET 240

Query: 241 KFNLVVAKDGSGNFTTVGEAVAAAPNSSTNRFVIYIKAGAYFENVEIERAKSNLMFVGDG 300
           KFNLVVAKDGSGNFTTV EAVAAAPNSST RFVIYIKAGAYFENVEI RAKSNLMFVGDG
Sbjct: 241 KFNLVVAKDGSGNFTTVNEAVAAAPNSSTTRFVIYIKAGAYFENVEIGRAKSNLMFVGDG 300

Query: 301 IGKTLIKANRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360
           IGKTLIK +RNVVDGWTTFRSATVAV+GTGFIAKGITFENYAGPSKHQAVALRSNSDFSA
Sbjct: 301 IGKTLIKGDRNVVDGWTTFRSATVAVIGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360

Query: 361 FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420
           FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF
Sbjct: 361 FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420

Query: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE 480
           TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE
Sbjct: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE 480

Query: 481 PVGWLEWNGTFALDTLYYGEYLNRGPGSNTTRRVTWPGYRVITNATEASQFTVEGFIQGS 540
           PVGWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITNATEASQFTVE FIQGS
Sbjct: 481 PVGWLEWNGTFALDTLYYGEYLNRGPGSNTTGRVTWPGYRVITNATEASQFTVEEFIQGS 540

Query: 541 SWLNSTGIPFFSGLASP 558
           SWLNSTGIPFFSGL SP
Sbjct: 541 SWLNSTGIPFFSGLTSP 557

BLAST of PI0005288 vs. NCBI nr
Match: XP_004136014.1 (pectinesterase isoform X2 [Cucumis sativus] >KGN45518.2 hypothetical protein Csa_015578 [Cucumis sativus])

HSP 1 Score: 1060.1 bits (2740), Expect = 6.7e-306
Identity = 528/561 (94.12%), Postives = 543/561 (96.79%), Query Frame = 0

Query: 1   MFGLKNRAHIPSNPTSISTIST---FFFIFFLFSFKPISSNPQIHQTARSLCNGTLFPNL 60
           M+GL+NRA I SNPTSISTIST   FFFIFFLFSFKPISSNPQ+HQTARSLCNGTLFP+L
Sbjct: 1   MYGLRNRAQISSNPTSISTISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDL 60

Query: 61  CFSKLSSFPQLASLSPEKLAGSALNFTTREVLLAYSNCTNLKTHLYRGLNPTDRHALDDC 120
           CFSKLSSFPQLASLSPEKLAGSALNFT+REVLLAY+NCTNLKTHLY GLNPTDRHALDDC
Sbjct: 61  CFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDC 120

Query: 121 LELLDDSIAELKASIFDLAPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180
           LELLD SIAELKASIFDLAPSQS A HSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV
Sbjct: 121 LELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180

Query: 181 ELYLGKIGQHLSIDLAMLKKIPGVNRATGV-EEVLPEYGAVKGGFPKWVSVKDRRLLQAA 240
           ELYLGKIGQHLSIDLAMLKKIPGVNRATGV +E+LPEYGAVKGGFPKWVSVKDRRLLQAA
Sbjct: 181 ELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLPEYGAVKGGFPKWVSVKDRRLLQAA 240

Query: 241 VNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTNRFVIYIKAGAYFENVEIERAKSNLMF 300
           VNETKFN+VVAKDGSGNFTTV EAVAAAPN+ST RFVIYIKAGAYFENVEI RAKSNLMF
Sbjct: 241 VNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRFVIYIKAGAYFENVEIGRAKSNLMF 300

Query: 301 VGDGIGKTLIKANRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNS 360
           VGDGIGKTLIKA+RNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNS
Sbjct: 301 VGDGIGKTLIKADRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNS 360

Query: 361 DFSAFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 420
           DFSAFYQCSFIGYQDTLYVHSLRQFYR+CD+YGTIDFIFGNAAVVFQNCNLYARKPNSNQ
Sbjct: 361 DFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYARKPNSNQ 420

Query: 421 RNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIG 480
           RNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKL+SRTVFLRSFIG
Sbjct: 421 RNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIG 480

Query: 481 QLVEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTRRVTWPGYRVITNATEASQFTVEGF 540
           QL+EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITNATEASQFTVE F
Sbjct: 481 QLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQFTVERF 540

Query: 541 IQGSSWLNSTGIPFFSGLASP 558
           IQGSSWLNST IPFFSGL  P
Sbjct: 541 IQGSSWLNSTEIPFFSGLTPP 561

BLAST of PI0005288 vs. NCBI nr
Match: XP_031744539.1 (pectinesterase isoform X1 [Cucumis sativus])

HSP 1 Score: 1053.5 bits (2723), Expect = 6.3e-304
Identity = 528/567 (93.12%), Postives = 543/567 (95.77%), Query Frame = 0

Query: 1   MFGLKNRAHIPSNPTSISTIST---FFFIFFLFSFKPISSNPQIHQTARSLCNGTLFPNL 60
           M+GL+NRA I SNPTSISTIST   FFFIFFLFSFKPISSNPQ+HQTARSLCNGTLFP+L
Sbjct: 1   MYGLRNRAQISSNPTSISTISTISPFFFIFFLFSFKPISSNPQVHQTARSLCNGTLFPDL 60

Query: 61  CFSKLSSFPQLASLSPEKLAGSALNFTTREVLLAYSNCTNLKTHLYRGLNPTDRHALDDC 120
           CFSKLSSFPQLASLSPEKLAGSALNFT+REVLLAY+NCTNLKTHLY GLNPTDRHALDDC
Sbjct: 61  CFSKLSSFPQLASLSPEKLAGSALNFTSREVLLAYTNCTNLKTHLYSGLNPTDRHALDDC 120

Query: 121 LELLDDSIAELKASIFDLAPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180
           LELLD SIAELKASIFDLAPSQS A HSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV
Sbjct: 121 LELLDGSIAELKASIFDLAPSQSPALHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRV 180

Query: 181 ELYLGKIGQHLSIDLAMLKKIPGVNRATGV-EEVLPEYGAVKGGFPKWVSVKDRRLLQAA 240
           ELYLGKIGQHLSIDLAMLKKIPGVNRATGV +E+LPEYGAVKGGFPKWVSVKDRRLLQAA
Sbjct: 181 ELYLGKIGQHLSIDLAMLKKIPGVNRATGVDQEMLPEYGAVKGGFPKWVSVKDRRLLQAA 240

Query: 241 VNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTNRFVIYIKAGAYFENVEIERAKSNLMF 300
           VNETKFN+VVAKDGSGNFTTV EAVAAAPN+ST RFVIYIKAGAYFENVEI RAKSNLMF
Sbjct: 241 VNETKFNMVVAKDGSGNFTTVSEAVAAAPNASTTRFVIYIKAGAYFENVEIGRAKSNLMF 300

Query: 301 VGDGIGKTLIKANRNVVDGWTTFRSAT------VAVVGTGFIAKGITFENYAGPSKHQAV 360
           VGDGIGKTLIKA+RNVVDGWTTFRSAT      VAVVGTGFIAKGITFENYAGPSKHQAV
Sbjct: 301 VGDGIGKTLIKADRNVVDGWTTFRSATVGFVYYVAVVGTGFIAKGITFENYAGPSKHQAV 360

Query: 361 ALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYAR 420
           ALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYR+CD+YGTIDFIFGNAAVVFQNCNLYAR
Sbjct: 361 ALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRDCDVYGTIDFIFGNAAVVFQNCNLYAR 420

Query: 421 KPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVF 480
           KPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKL+SRTVF
Sbjct: 421 KPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVF 480

Query: 481 LRSFIGQLVEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTRRVTWPGYRVITNATEASQ 540
           LRSFIGQL+EPVGWLEWNGTFALDTLYYGEYLNRGPGSNTT RVTWPGYRVITNATEASQ
Sbjct: 481 LRSFIGQLIEPVGWLEWNGTFALDTLYYGEYLNRGPGSNTTMRVTWPGYRVITNATEASQ 540

Query: 541 FTVEGFIQGSSWLNSTGIPFFSGLASP 558
           FTVE FIQGSSWLNST IPFFSGL  P
Sbjct: 541 FTVERFIQGSSWLNSTEIPFFSGLTPP 567

BLAST of PI0005288 vs. NCBI nr
Match: XP_038896904.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 1052.7 bits (2721), Expect = 1.1e-303
Identity = 521/555 (93.87%), Postives = 535/555 (96.40%), Query Frame = 0

Query: 1   MFGLKNRAHIPSNPTSISTISTFFFIFFLFSFKPISSNPQIHQTARSLCNGTLFPNLCFS 60
           M+GLKNRA IPSNPTSISTISTFFFIFF FSFKPISSNPQIHQTARSLCNGTLFP+LCFS
Sbjct: 1   MYGLKNRAQIPSNPTSISTISTFFFIFFFFSFKPISSNPQIHQTARSLCNGTLFPHLCFS 60

Query: 61  KLSSFPQLASLSPEKLAGSALNFTTREVLLAYSNCTNLKTHLYRGLNPTDRHALDDCLEL 120
           KLSSFPQLASLSPEKLAGSA+NFTTREVL AY+NCT LKT LY GLNPTDR ALDDCLEL
Sbjct: 61  KLSSFPQLASLSPEKLAGSAINFTTREVLGAYANCTKLKTILYGGLNPTDRRALDDCLEL 120

Query: 121 LDDSIAELKASIFDLAPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELY 180
           LD SIAEL+ASI DLAP QSAARHSHDLLTL+SAAMTNHRTCVDGFYNSSGTVRSRVELY
Sbjct: 121 LDSSIAELQASICDLAPPQSAARHSHDLLTLISAAMTNHRTCVDGFYNSSGTVRSRVELY 180

Query: 181 LGKIGQHLSIDLAMLKKIPGVNRATGVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNET 240
           LGKIGQHLSIDLAMLKKIPGVNRA    EVLPEYGAVKGGFP WVSVKDRRLLQAAVNET
Sbjct: 181 LGKIGQHLSIDLAMLKKIPGVNRAPAAGEVLPEYGAVKGGFPNWVSVKDRRLLQAAVNET 240

Query: 241 KFNLVVAKDGSGNFTTVGEAVAAAPNSSTNRFVIYIKAGAYFENVEIERAKSNLMFVGDG 300
           KFNLVVAKDGSGNFTTVGEAVAAAPNSST RFVIYIKAGAYFENVEIER K+NLMFVGDG
Sbjct: 241 KFNLVVAKDGSGNFTTVGEAVAAAPNSSTTRFVIYIKAGAYFENVEIERRKTNLMFVGDG 300

Query: 301 IGKTLIKANRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360
           IGKTLIKA+RNVVDGWTTFRSATVAVVG GFIAKGITFENYAGPSKHQAVALRSNSDFSA
Sbjct: 301 IGKTLIKADRNVVDGWTTFRSATVAVVGNGFIAKGITFENYAGPSKHQAVALRSNSDFSA 360

Query: 361 FYQCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420
           FYQCSF+GYQDTLYVHSLRQFYR+CDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF
Sbjct: 361 FYQCSFVGYQDTLYVHSLRQFYRDCDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIF 420

Query: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVE 480
           TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKL+SRTVFLRSFIGQLV+
Sbjct: 421 TAQGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLYSRTVFLRSFIGQLVD 480

Query: 481 PVGWLEWNGTFALDTLYYGEYLNRGPGSNTTRRVTWPGYRVITNATEASQFTVEGFIQGS 540
           P GWLEWNGTFALDTLYYGEY+NRGPGSNTT RVTWPGYRVITNA+EASQFTVEGFIQGS
Sbjct: 481 PAGWLEWNGTFALDTLYYGEYMNRGPGSNTTGRVTWPGYRVITNASEASQFTVEGFIQGS 540

Query: 541 SWLNSTGIPFFSGLA 556
           SWLNSTGIPFFSGLA
Sbjct: 541 SWLNSTGIPFFSGLA 555

BLAST of PI0005288 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 543.9 bits (1400), Expect = 1.5e-154
Identity = 278/488 (56.97%), Postives = 351/488 (71.93%), Query Frame = 0

Query: 75  KLAGSALNFTTREVLLAYSNCTNLKTHLYRGLNPTDRHALDDCLELLDDSIAELKASIFD 134
           +L  +ALN T   V L+ SN ++L   L   L+  D  A DDCLELLDD++ +L  +I  
Sbjct: 39  ELVVTALNQTISNVNLSSSNFSDLLQRLGSNLSHRDLCAFDDCLELLDDTVFDLTTAISK 98

Query: 135 LAPSQSAARHSHDLLTLVSAAMTNHRTCVDGFYNSSGT------------VRSRVELYLG 194
           L   +S +   H++  L+SAAMTN RTC+DGF +S               V   ++  L 
Sbjct: 99  L---RSHSPELHNVKMLLSAAMTNTRTCLDGFASSDNDENLNNNDNKTYGVAESLKESLF 158

Query: 195 KIGQHLSIDLAMLKKIPGVNRATGVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETKF 254
            I  H+S  LAML+ IPG       E+V         GFP WVS  DR LLQ  V+ETK 
Sbjct: 159 NISSHVSDSLAMLENIPGHIPGKVKEDV---------GFPMWVSGSDRNLLQDPVDETKV 218

Query: 255 NLVVAKDGSGNFTTVGEAVAAAPNSSTNRFVIYIKAGAYFENVEIERAKSNLMFVGDGIG 314
           NLVVA++G+GN+TT+GEA++AAPNSS  RFVIYIK G YFEN+EI R K+ +MF+GDGIG
Sbjct: 219 NLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIG 278

Query: 315 KTLIKANRNVVDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFY 374
           +T+IKANR+  DGWT F SATV V G+GFIAK ++F NYAGP KHQAVALRS+SD SA+Y
Sbjct: 279 RTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSSDLSAYY 338

Query: 375 QCSFIGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTA 434
           +CSF  YQDT+YVHS +QFYRECDIYGT+DFIFG+A+VVFQNC+LYAR+PN NQ+ I+TA
Sbjct: 339 RCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQKIIYTA 398

Query: 435 QGREDPNQNTGISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVEPV 494
           QGRE+  + TGISI++ ++ AA DLIPV ++F+ YLGRPW+L+SRTV ++SFI  LV+P 
Sbjct: 399 QGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFIDDLVDPA 458

Query: 495 GWLEWNGTFALDTLYYGEYLNRGPGSNTTRRVTWPGYRVITNATEASQFTVEGFIQGSSW 551
           GWL+W   FAL+TLYYGEY+N GPGSN T RV WPG++ I    EASQF+V  FI G+ W
Sbjct: 459 GWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGNKW 514

BLAST of PI0005288 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 516.5 bits (1329), Expect = 2.6e-146
Identity = 267/481 (55.51%), Postives = 337/481 (70.06%), Query Frame = 0

Query: 81  LNFTTREVLLAYSNCTNLKTHLYRGLNPTDRHALDDCLELLDDSIAELKASIFDLAPSQS 140
           LN T  +V LA SN ++L+T L+  L   +R A +DCL LLDD+I++L+ ++ DL   +S
Sbjct: 61  LNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISDLETAVSDL---RS 120

Query: 141 AARHSHDLLTLVSAAMTNHRTCVDGFYNSSGTVRSRVELYLGK--------IGQHLSIDL 200
           ++   +D+  L++  MT   TC+DGF  S     + +   L +        I  +LS  L
Sbjct: 121 SSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPENLKEIILDISNNLSNSL 180

Query: 201 AMLKKIPGVNRATGVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSG 260
            ML+ I     +    EV  EY       P W+S  D+RLL+A V ET +NL VA DG+G
Sbjct: 181 HMLQVISRKKPSPKSSEVDVEY-------PSWLSENDQRLLEAPVQETNYNLSVAIDGTG 240

Query: 261 NFTTVGEAVAAAPNSSTNRFVIYIKAGAYFENVEIERAKSNLMFVGDGIGKTLIKANRNV 320
           NFTT+ +AV AAPN S  RF+IYIK G YFENVE+ + K+ +MF+GDGIGKT+IKANR+ 
Sbjct: 241 NFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANRSR 300

Query: 321 VDGWTTFRSATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDT 380
           +DGW+TF++ TV V G G+IAK I+F N AGP+K QAVA RS SD SAFY+C F GYQDT
Sbjct: 301 IDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEFDGYQDT 360

Query: 381 LYVHSLRQFYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNT 440
           LYVHS +QFYRECDIYGTIDFIFGNAAVVFQN +LYARKPN   +  FTAQ R   +Q T
Sbjct: 361 LYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRNQSDQPT 420

Query: 441 GISILNCKVEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVEPVGWLEWNGTFA 500
           GISILNC++ AA DLIPV  +F+ YLGRPW+ +SRTV ++SFI  L+ P GWLE    FA
Sbjct: 421 GISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEGKKDFA 480

Query: 501 LDTLYYGEYLNRGPGSNTTRRVTWPGYRVITNATEASQFTVEGFIQGSSWLNSTGIPFFS 554
           L+TLYYGEY+N GPG+N  +RVTWPG+R I N TEA+QFTV  FI GS+WLNSTGIPF  
Sbjct: 481 LETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFSL 531

BLAST of PI0005288 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 499.6 bits (1285), Expect = 3.3e-141
Identity = 271/530 (51.13%), Postives = 348/530 (65.66%), Query Frame = 0

Query: 42  HQTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTTREVLLAYSNCTNLKTH 101
           H   +S+C+ TL+P LCFS +++       S +++  ++LN TT+ V   Y     L   
Sbjct: 59  HAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAK 118

Query: 102 LYRGLNPTDRHALDDCLELLDDSIAELKASIFDL--APSQSAAR-HSHDLLTLVSAAMTN 161
             +GL P +  AL DCLE +D+++ EL  ++ DL   P Q + R H+ DL TL+S+A+TN
Sbjct: 119 -RKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITN 178

Query: 162 HRTCVDGFYNSSGTVRSRVELYLGKIG-QHL-SIDLAMLKKIPGV--------------- 221
             TC+DGF       + R  L  G++  +H+ S  LAM+K +                  
Sbjct: 179 QGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFT 238

Query: 222 -NRATGVEEVLPEYGAVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEA 281
            N    ++EV  +  +   G+PKW+SV DRRLLQ +    K +  VA DGSG+FTTV  A
Sbjct: 239 NNNNRKLKEVTGDLDS--DGWPKWLSVGDRRLLQGST--IKADATVADDGSGDFTTVAAA 298

Query: 282 VAAAPNSSTNRFVIYIKAGAYFENVEIERAKSNLMFVGDGIGKTLIKANRNVVDGWTTFR 341
           VAAAP  S  RFVI+IKAG Y ENVE+ + K+N+MF+GDG GKT+I  +RNVVDG TTF 
Sbjct: 299 VAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFH 358

Query: 342 SATVAVVGTGFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQ 401
           SATVA VG  F+A+ ITF+N AGPSKHQAVALR  SDFSAFYQC    YQDTLYVHS RQ
Sbjct: 359 SATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQ 418

Query: 402 FYRECDIYGTIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCK 461
           F+ +C I GT+DFIFGNAA V Q+C++ AR+PNS Q+N+ TAQGR DPNQNTGI I NC+
Sbjct: 419 FFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCR 478

Query: 462 VEAASDLIPVLSSFRTYLGRPWKLHSRTVFLRSFIGQLVEPVGWLEWNGTFALDTLYYGE 521
           +   SDL+ V  +F TYLGRPWK +SRTV ++S I  ++ P GW EW+G+FALDTL Y E
Sbjct: 479 IGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYRE 538

Query: 522 YLNRGPGSNTTRRVTWPGYRVITNATEASQFTVEGFIQGSSWLNSTGIPF 551
           YLNRG G+ T  RV W GY+VIT+ TEA  FT   FI G  WL STG PF
Sbjct: 539 YLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPF 583

BLAST of PI0005288 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 479.2 bits (1232), Expect = 4.6e-135
Identity = 259/526 (49.24%), Postives = 338/526 (64.26%), Query Frame = 0

Query: 43  QTARSLCNGTLFPNLCFSKLSSFP-QLASLSPEKLAGSALNFTTREVLLAYSNCTNLKTH 102
           Q     C  T FP LC   L  FP  LA+ S + L    +N T      A  +  +L   
Sbjct: 85  QAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLS-- 144

Query: 103 LYRGLNPTDRHALDDCLELLDDSIAELKASIFDLAPSQSAARHSHDLLTLVSAAMTNHRT 162
            +  + P  R A D C+ELLDDS+  L  ++  +    S++    D+ T +SAA+TNH T
Sbjct: 145 -FVDMPPRARSAYDSCVELLDDSVDALSRALSSVV---SSSAKPQDVTTWLSAALTNHDT 204

Query: 163 CVDGFYN-SSGTVRSRVELYLGKIGQHLSIDLAMLKK---------IPGVN-RATGVEEV 222
           C +GF     G V+  +   L  + + +S  LA+            +P  N R  GVEE 
Sbjct: 205 CTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEE- 264

Query: 223 LPEYGAVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTN 282
                  +  FP+W+  K+R +L+  V++ + +++V+KDG+G   T+ EA+  AP +ST 
Sbjct: 265 ------REEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTR 324

Query: 283 RFVIYIKAGAYFE-NVEIERAKSNLMFVGDGIGKTLIKANRNVVDGWTTFRSATVAVVGT 342
           R +IY+KAG Y E N+++ R K NLMFVGDG GKT+I   +++ D  TTF +A+ A  G 
Sbjct: 325 RIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGA 384

Query: 343 GFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDIYG 402
           GFIA+ ITFEN+AGP+KHQAVALR  +D +  Y+C+ IGYQDTLYVHS RQF+RECDIYG
Sbjct: 385 GFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYG 444

Query: 403 TIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIP 462
           T+DFIFGNAAVV QNC++YARKP   Q+N  TAQ R+DPNQNTGISI   +V AASDL  
Sbjct: 445 TVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQA 504

Query: 463 VLSSFRTYLGRPWKLHSRTVFLRSFIGQLVEPVGWLEWNGTFALDTLYYGEYLNRGPGSN 522
              S +TYLGRPWKL SRTV++ S+IG  V   GWLEWN TFALDTLYYGEYLN GPGS 
Sbjct: 505 TNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSG 564

Query: 523 TTRRVTWPGYRVITNATEASQFTVEGFIQGSSWLNSTGIPFFSGLA 556
             +RV+WPGYRVI +  EA++FTV  FI GSSWL STG+ F +GL+
Sbjct: 565 LGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

BLAST of PI0005288 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 478.8 bits (1231), Expect = 6.0e-135
Identity = 254/526 (48.29%), Postives = 333/526 (63.31%), Query Frame = 0

Query: 43  QTARSLCNGTLFPNLCFSKLSSFPQLASLSPEKLAGSALNFTTREVLLAYSNCTNLKTHL 102
           Q     C+ +L+PNLC   L  FP   +    +L   + N T ++   A    +   T  
Sbjct: 73  QAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS---TIT 132

Query: 103 YRGLNPTDRHALDDCLELLDDSIAELKASIFDLAPSQSAARHSHDLLTLVSAAMTNHRTC 162
           Y  + P  R A D CLELLDDS+  L  ++  +        HS D++T +S+AMTNH TC
Sbjct: 133 YTQMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHS-DVMTWLSSAMTNHDTC 192

Query: 163 VDGF---YNSSGTVRSRVELYLGKIGQHLSIDLAM-------LKKIPGVN--RATGVEEV 222
            DGF       G V+ +V   +  + + +S  LA+       L  +P VN  +  G EE 
Sbjct: 193 TDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEET 252

Query: 223 LPEYGAVKGGFPKWVSVKDRRLLQAAVNETKFNLVVAKDGSGNFTTVGEAVAAAPNSSTN 282
                      P W+  +DR LL    +  + ++ V+KDGSG F T+ EA+  AP  S+ 
Sbjct: 253 --------EELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSR 312

Query: 283 RFVIYIKAGAY-FENVEIERAKSNLMFVGDGIGKTLIKANRNVVDGWTTFRSATVAVVGT 342
           RFVIY+KAG Y  EN+++ R K+NLMF+GDG GKT+I   +++ D  TTF +AT A  G 
Sbjct: 313 RFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGA 372

Query: 343 GFIAKGITFENYAGPSKHQAVALRSNSDFSAFYQCSFIGYQDTLYVHSLRQFYRECDIYG 402
           GFI + +TFENYAGP+KHQAVALR   D +  Y+C+ IGYQD LYVHS RQF+REC+IYG
Sbjct: 373 GFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYG 432

Query: 403 TIDFIFGNAAVVFQNCNLYARKPNSNQRNIFTAQGREDPNQNTGISILNCKVEAASDLIP 462
           T+DFIFGNAAV+ Q+CN+YARKP + Q+   TAQ R+DPNQNTGISI  CK+ A  DL  
Sbjct: 433 TVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEA 492

Query: 463 VLSSFRTYLGRPWKLHSRTVFLRSFIGQLVEPVGWLEWNGTFALDTLYYGEYLNRGPGSN 522
              S+ TYLGRPWKL+SR V++ S +G  ++P GWLEWNG FALD+LYYGEY+N+G GS 
Sbjct: 493 SKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSG 552

Query: 523 TTRRVTWPGYRVITNATEASQFTVEGFIQGSSWLNSTGIPFFSGLA 556
             +RV WPGY VIT+  EAS+FTV  FI GSSWL STG+ FFSGL+
Sbjct: 553 IGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O813012.1e-15356.97Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3... [more]
O814153.6e-14555.51Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3... [more]
P850761.2e-14377.43Pectinesterase OS=Actinidia deliciosa OX=3627 PE=1 SV=1[more]
Q425344.6e-14051.13Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
P839481.9e-13850.19Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BSU30.0e+0096.77Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492792 PE=3 SV=1[more]
A0A5D3D1W60.0e+0096.59Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00130... [more]
A0A6J1JU831.2e-28989.93Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487942 PE=3 SV=1[more]
A0A6J1GNU41.7e-28688.85Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456156 PE=3 SV=1[more]
A0A0A0K5K83.8e-28693.90Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405320 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008451545.20.0e+0096.77PREDICTED: pectinesterase-like [Cucumis melo][more]
KAA0046658.10.0e+0096.59pectinesterase-like [Cucumis melo var. makuwa] >TYK18193.1 pectinesterase-like [... [more]
XP_004136014.16.7e-30694.12pectinesterase isoform X2 [Cucumis sativus] >KGN45518.2 hypothetical protein Csa... [more]
XP_031744539.16.3e-30493.12pectinesterase isoform X1 [Cucumis sativus][more]
XP_038896904.11.1e-30393.87pectinesterase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G02320.11.5e-15456.97Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02300.12.6e-14655.51Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G53830.13.3e-14151.13pectin methylesterase 2 [more]
AT3G49220.14.6e-13549.24Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G53370.16.0e-13548.29pectin methylesterase PCR fragment F [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 40..194
e-value: 2.7E-29
score: 113.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 46..177
e-value: 4.6E-23
score: 82.2
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 20..197
e-value: 7.5E-24
score: 82.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 44..200
e-value: 2.4E-27
score: 97.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 46..199
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 243..539
e-value: 5.5E-145
score: 481.7
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 237..554
e-value: 2.1E-123
score: 413.8
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 42..551
NoneNo IPR availablePANTHERPTHR31707:SF257PECTINESTERASEcoord: 42..551
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 47..200
e-value: 1.61714E-24
score: 97.894
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 387..396
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 241..553

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0005288.1PI0005288.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity