PI0004966 (gene) Melon (PI 482460) v1

BLAST of PI0004966 vs. ExPASy Swiss-Prot
Match: P43297 (Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 1.2e-162
Identity = 288/451 (63.86%), Postives = 335/451 (74.28%), Query Frame = 0

Query: 13  LFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAALYESWLVHHGKA 72
           LFL +   SSA      SII  + KH +       S    R++ EV ++YE+WLV HGKA
Sbjct: 11  LFLAMVAVSSA---VDMSIISYDEKHGV-------STTGGRSEAEVMSIYEAWLVKHGKA 70

Query: 73  --YNAIGEKERRFEIFKDNLRFIDEHNRETRTYKVGLTRFADLTNEEYRASLSAPK---- 132
              N++ EK+RRFEIFKDNLRF+DEHN +  +Y++GLTRFADLTN+EYR+     K    
Sbjct: 71  QSQNSLVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKK 130

Query: 133 -----SGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSTVAAVEGINQIVTGE 192
                S RY A +GD+LP+ +DWRKKGAVA VKDQG CGSCWAFST+ AVEGINQIVTG+
Sbjct: 131 GERRTSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGD 190

Query: 193 LISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTDQDYPYKGRDGACDPNR--- 252
           LI+LSEQELVDCD SYN GCNGGLMDYAF+FII NGGIDTD+DYPYKG DG CD  R   
Sbjct: 191 LITLSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNA 250

Query: 253 -VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGTNLDHGVV 312
            VVTID YEDVP   E SLKKAVA+QP+S+AIEAGGRAFQLY SG+F G CGT LDHGVV
Sbjct: 251 KVVTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHGVV 310

Query: 313 TVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANITTGKCGIAVEPSYPIKTGSNPP 372
            VGYGTENG DYWIVRNSWGK WGE+GY+R+ RN+A+ ++GKCGIA+EPSYPIK G NPP
Sbjct: 311 AVGYGTENGKDYWIVRNSWGKSWGESGYLRMARNIAS-SSGKCGIAIEPSYPIKNGENPP 370

Query: 373 KPSASPPSPVKPPTECDEY------------FSLWKHLLWLGMLPLESATCCDDHYSCCP 432
            P  SPPSP+KPPT+CD Y            F   K+    G  PLE+ATCCDD+YSCCP
Sbjct: 371 NPGPSPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNYSCCP 430

Query: 433 HEYPVCDLEAGTCRVSKDSSMGVNLLKRLPA 437
           HEYPVCDL+ GTC +SK+S   V  LKR PA
Sbjct: 431 HEYPVCDLDQGTCLLSKNSPFSVKALKRKPA 450

BLAST of PI0004966 vs. ExPASy Swiss-Prot
Match: Q9FMH8 (Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 5.6e-160
Identity = 287/457 (62.80%), Postives = 329/457 (71.99%), Query Frame = 0

Query: 8   FAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAALYESWLV 67
           F  L+  + L      S++   SII  +  HH+       +    R+D EV  +YE+W+V
Sbjct: 3   FLKLSPMILLLAMIGVSYAMDMSIISYDENHHI-------TTETSRSDSEVERIYEAWMV 62

Query: 68  HHGKA---YNAIG-EKERRFEIFKDNLRFIDEHNRETRTYKVGLTRFADLTNEEYRASLS 127
            HGK     N +G EK++RFEIFKDNLRFIDEHN +  +YK+GLTRFADLTNEEYR+   
Sbjct: 63  EHGKKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYL 122

Query: 128 APK--------SGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSTVAAVEGIN 187
             K        S RY A +GD LPD VDWRK+GAVA VKDQG CGSCWAFST+ AVEGIN
Sbjct: 123 GAKPTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGIN 182

Query: 188 QIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTDQDYPYKGRDGACD 247
           +IVTG+LISLSEQELVDCD SYN GCNGGLMDYAF+FII NGGIDT+ DYPYK  DG CD
Sbjct: 183 KIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCD 242

Query: 248 PNR----VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGTN 307
            NR    VVTID YEDVPEN E+SLKKA+A+QP+SVAIEAGGRAFQLY SGVF G CGT 
Sbjct: 243 QNRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTE 302

Query: 308 LDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANITTGKCGIAVEPSYPIK 367
           LDHGVV VGYGTENG DYWIVRNSWG  WGE+GYI++ RN+    TGKCGIA+E SYPIK
Sbjct: 303 LDHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNI-EAPTGKCGIAMEASYPIK 362

Query: 368 TGSNPPKPSASPPSPVKPPTECDEYFS------------LWKHLLWLGMLPLESATCCDD 427
            G NPP P  SPPSP+KPPT CD+YFS              K+    G  PLE+ATCCDD
Sbjct: 363 KGQNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAATCCDD 422

Query: 428 HYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPA 437
           + SCCPHEYPVCD+  GTC +SK+S   V  LKR PA
Sbjct: 423 NSSCCPHEYPVCDVNRGTCLMSKNSPFSVKALKRTPA 451

BLAST of PI0004966 vs. ExPASy Swiss-Prot
Match: P25776 (Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=1 SV=2)

HSP 1 Score: 549.3 bits (1414), Expect = 4.1e-155
Identity = 266/415 (64.10%), Postives = 312/415 (75.18%), Query Frame = 0

Query: 52  QRTDEEVAALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRE----TRTYKVGL 111
           +R++EE   LY  W   HGK+YNA+GE+ERR+  F+DNLR+IDEHN        ++++GL
Sbjct: 30  ERSEEEARRLYAEWKAEHGKSYNAVGEEERRYAAFRDNLRYIDEHNAAADAGVHSFRLGL 89

Query: 112 TRFADLTNEEYRASL----SAPK-----SGRYAAALGDDLPDDVDWRKKGAVATVKDQGQ 171
            RFADLTNEEYR +     + P+     S RY AA  + LP+ VDWR KGAVA +KDQG 
Sbjct: 90  NRFADLTNEEYRDTYLGLRNKPRRERKVSDRYLAADNEALPESVDWRTKGAVAEIKDQGG 149

Query: 172 CGSCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGG 231
           CGSCWAFS +AAVEGINQIVTG+LISLSEQELVDCD SYN GCNGGLMDYAF FII+NGG
Sbjct: 150 CGSCWAFSAIAAVEGINQIVTGDLISLSEQELVDCDTSYNEGCNGGLMDYAFDFIINNGG 209

Query: 232 IDTDQDYPYKGRDGACDPNR----VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGR 291
           IDT+ DYPYKG+D  CD NR    VVTID YEDV  N E+SL+KAVANQPVSVAIEAGGR
Sbjct: 210 IDTEDDYPYKGKDERCDVNRKNAKVVTIDSYEDVTPNSETSLQKAVANQPVSVAIEAGGR 269

Query: 292 AFQLYQSGVFTGRCGTNLDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVAN 351
           AFQLY SG+FTG+CGT LDHGV  VGYGTENG DYWIVRNSWGK WGE+GY+R+ERN+  
Sbjct: 270 AFQLYSSGIFTGKCGTALDHGVAAVGYGTENGKDYWIVRNSWGKSWGESGYVRMERNI-K 329

Query: 352 ITTGKCGIAVEPSYPIKTGSNPPKPSASPPSPVKPPTECDEYFS------------LWKH 411
            ++GKCGIAVEPSYP+K G NPP P  +PPSP  PPT CD Y++              K+
Sbjct: 330 ASSGKCGIAVEPSYPLKKGENPPNPGPTPPSPTPPPTVCDNYYTCPDSTTCCCIYEYGKY 389

Query: 412 LLWLGMLPLESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAK 438
               G  PLE ATCCDDHYSCCPHEYP+C+++ GTC ++KDS + V  LKR  AK
Sbjct: 390 CYAWGCCPLEGATCCDDHYSCCPHEYPICNVQQGTCLMAKDSPLAVKALKRTLAK 443

BLAST of PI0004966 vs. ExPASy Swiss-Prot
Match: Q9LT78 (Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 1.9e-139
Identity = 258/414 (62.32%), Postives = 298/414 (71.98%), Query Frame = 0

Query: 53  RTDEEVAALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNR-ETRTYKVGLTRFA 112
           R + E   +YE WLV + K YN +GEKERRFEIFKDNL+F++EH+    RTY+VGLTRFA
Sbjct: 34  RNEAEARRMYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFA 93

Query: 113 DLTNEEYRASLSAPKSGR---------YAAALGDDLPDDVDWRKKGAVATVKDQGQCGSC 172
           DLTN+E+RA     K  R         Y   +GD LPD +DWR KGAV  VKDQG CGSC
Sbjct: 94  DLTNDEFRAIYLRSKMERTRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSC 153

Query: 173 WAFSTVAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTD 232
           WAFS + AVEGINQI TGELISLSEQELVDCD SYN GC GGLMDYAF+FII+NGGIDT+
Sbjct: 154 WAFSAIGAVEGINQIKTGELISLSEQELVDCDTSYNDGCGGGLMDYAFKFIIENGGIDTE 213

Query: 233 QDYPYKGRD-GACDPN----RVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQ 292
           +DYPY   D   C+ +    RVVTIDGYEDVP+NDE SLKKA+ANQP+SVAIEAGGRAFQ
Sbjct: 214 EDYPYIATDVNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQ 273

Query: 293 LYQSGVFTGRCGTNLDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANITT 352
           LY SGVFTG CGT+LDHGVV VGYG+E G DYWIVRNSWG +WGE+GY +LERN+   ++
Sbjct: 274 LYTSGVFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKE-SS 333

Query: 353 GKCGIAVEPSYPIK-TGSNPPKPSASPPSPV---------KPPTECDEYFSLWKHLLWLG 412
           GKCG+A+  SYP K +GSNPPKP A  PSPV            T C  Y    K   W G
Sbjct: 334 GKCGVAMMASYPTKSSGSNPPKPPA--PSPVVCDKSNTCPAKSTCCCLYEYNGKCYSW-G 393

Query: 413 MLPLESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAKQTKR 442
             P ESATCCDD  SCCP  YPVCDL+A TCR+  +S + +  L R PA  T +
Sbjct: 394 CCPYESATCCDDGSSCCPQSYPVCDLKANTCRMKGNSPLSIKALTRGPAIATTK 443

BLAST of PI0004966 vs. ExPASy Swiss-Prot
Match: P25777 (Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1 SV=2)

HSP 1 Score: 489.2 bits (1258), Expect = 5.1e-137
Identity = 264/466 (56.65%), Postives = 322/466 (69.10%), Query Frame = 0

Query: 9   AFLALFLCLSPFSSASHSSTFSIIDENAKHHL-GIPEIPHSDAHQRTDEEVAALYESWLV 68
           A  A FL L    +A+ +   SII  NA+H   G+ E P       T+ E  A Y+ WL 
Sbjct: 5   AAAAAFLLLLIVGAATAAPDMSIISYNAEHGARGLEEGP-------TEAEARAAYDLWLA 64

Query: 69  HH-GKAYNAI-GEKERRFEIFKDNLRFIDEHNR---ETRTYKVGLTRFADLTNEEYRASL 128
            + G + NA+ GE ERRF +F DNL+F+D HN    E   +++G+ RFADLTNEE+RA+ 
Sbjct: 65  ENGGGSPNALGGEHERRFLVFWDNLKFVDAHNARADERGGFRLGMNRFADLTNEEFRATF 124

Query: 129 SAPKSGRYAAALG--------DDLPDDVDWRKKGAVATVKDQGQCGSCWAFSTVAAVEGI 188
              K    + A G        ++LP+ VDWR+KGAVA VK+QGQCGSCWAFS V+ VE I
Sbjct: 125 LGAKVAERSRAAGERYRHDGVEELPESVDWREKGAVAPVKNQGQCGSCWAFSAVSTVESI 184

Query: 189 NQIVTGELISLSEQELVDCD-KSYNMGCNGGLMDYAFQFIIDNGGIDTDQDYPYKGRDGA 248
           NQ+VTGE+I+LSEQELV+C     N GCNGGLMD AF FII NGGIDT+ DYPYK  DG 
Sbjct: 185 NQLVTGEMITLSEQELVECSTNGQNSGCNGGLMDDAFDFIIKNGGIDTEDDYPYKAVDGK 244

Query: 249 CDPNR----VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCG 308
           CD NR    VV+IDG+EDVP+NDE SL+KAVA+QPVSVAIEAGGR FQLY SGVF+GRCG
Sbjct: 245 CDINRENAKVVSIDGFEDVPQNDEKSLQKAVAHQPVSVAIEAGGREFQLYHSGVFSGRCG 304

Query: 309 TNLDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANITTGKCGIAVEPSYP 368
           T+LDHGVV VGYGT+NG DYWIVRNSWG  WGE+GY+R+ERN+ N+TTGKCGIA+  SYP
Sbjct: 305 TSLDHGVVAVGYGTDNGKDYWIVRNSWGPKWGESGYVRMERNI-NVTTGKCGIAMMASYP 364

Query: 369 IKTGSNPPKPSASPPSPVKPPTE------CDEYFSL-----------WKHL-LWLGMLPL 428
            K+G+NPPKPS +PP+P  PP        CD+ FS            +++L L  G  P+
Sbjct: 365 TKSGANPPKPSPTPPTPPTPPPPSAPDHVCDDNFSCPAGSTCCCAFGFRNLCLVWGCCPV 424

Query: 429 ESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAK 438
           E ATCC DH SCCP +YPVC+  AGTC  SK+S + V  LKR  AK
Sbjct: 425 EGATCCKDHASCCPPDYPVCNTRAGTCSASKNSPLSVKALKRTLAK 462

BLAST of PI0004966 vs. ExPASy TrEMBL
Match: A0A1S3BZW8 (low-temperature-induced cysteine proteinase-like OS=Cucumis melo OX=3656 GN=LOC103495259 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 3.8e-244
Identity = 428/481 (88.98%), Postives = 438/481 (91.06%), Query Frame = 0

Query: 1   MAI-SPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60
           MAI SPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA
Sbjct: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60

Query: 61  ALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRETRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNA+GEKERRFEIFKDNL FIDEHNRE+RTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 A-----------SLSAPKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSTV 180
           A           SLSA KSGRYAAALGDDLPDDVDWRKKGAVA VKDQGQCGSCWAFSTV
Sbjct: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV 180

Query: 181 AAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTDQDYPYK 240
           AAVEGINQIVTGELISLSEQELVDCDKS+NMGCNGGLMDYAFQFIIDNGGIDTD+DYPYK
Sbjct: 181 AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK 240

Query: 241 GRDGACDPNR----VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300
           GRDGACDPNR    VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF
Sbjct: 241 GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300

Query: 301 TGRCGTNLDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANITTGKCGIAV 360
           TGRCGTNLDHGVV VGYGT+NGTDYWIVRNSWGKDWGENGYIRLERNVAN TTGKCGIAV
Sbjct: 301 TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV 360

Query: 361 EPSYPIKTGSNPPKPSASPPSPVKPPTECDEYFS---------LWKH----LLWLGMLPL 420
           EPSYPIK+GSNPPKPSASPPSPV PPTECDEYFS         ++++      W G  PL
Sbjct: 361 EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAW-GCCPL 420

Query: 421 ESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAKQTKRIQKLGKLFVG 453
           ESATCCDDHYSCCPHEYPVCDLEAGTCR SKDS MGVNLLKRLPA QTKRIQKLGKLFVG
Sbjct: 421 ESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQKLGKLFVG 480

BLAST of PI0004966 vs. ExPASy TrEMBL
Match: A0A0A0K4N3 (Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_7G073400 PE=3 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 2.3e-241
Identity = 420/480 (87.50%), Postives = 434/480 (90.42%), Query Frame = 0

Query: 1   MAISPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAA 60
           MAISPIFFAFLALF CLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQR DEEVAA
Sbjct: 1   MAISPIFFAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVAA 60

Query: 61  LYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRETRTYKVGLTRFADLTNEEYRA 120
           LYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRE+RTYKVGLTRFADLTNEEYRA
Sbjct: 61  LYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYRA 120

Query: 121 -----------SLSAPKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSTVA 180
                       LSA KSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFS+VA
Sbjct: 121 RFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSVA 180

Query: 181 AVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTDQDYPYKG 240
           AVEGINQIVTGELI LSEQELVDCDKS+NMGCNGGLMDYAFQFII NGGIDT++DYPYKG
Sbjct: 181 AVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYKG 240

Query: 241 RDGACDPNR----VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFT 300
           RD ACDPNR    VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFT
Sbjct: 241 RDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFT 300

Query: 301 GRCGTNLDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANITTGKCGIAVE 360
           GRCGT+LDHGVV VGYGT+NGTDYWIVRNSWGKDWGE+GYIRLERNVANITTGKCGIAV+
Sbjct: 301 GRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAVQ 360

Query: 361 PSYPIKTGSNPPKPSASPPSPVKPPTECDEYFSLWKH-------------LLWLGMLPLE 420
           PSYP K+G+NPPKPSASPPSPVKPPTECDEYFS  +                W G  PLE
Sbjct: 361 PSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAW-GCCPLE 420

Query: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAKQTKRIQKLGKLFVGA 453
           SATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPA QTK++QKLGKLFVGA
Sbjct: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTKKVQKLGKLFVGA 479

BLAST of PI0004966 vs. ExPASy TrEMBL
Match: A0A5A7SJU5 (Low-temperature-induced cysteine proteinase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00750 PE=3 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 8.7e-241
Identity = 427/489 (87.32%), Postives = 437/489 (89.37%), Query Frame = 0

Query: 1   MAI-SPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60
           MAI SPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA
Sbjct: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60

Query: 61  ALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRETRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNA+GEKERRFEIFKDNL FIDEHNRE+RTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 A-----------SLSAPKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQ--------CG 180
           A           SLSA KSGRYAAALGDDLPDDVDWRKKGAVA VKDQGQ         G
Sbjct: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQKFVMRSIFIG 180

Query: 181 SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGID 240
           SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKS+NMGCNGGLMDYAFQFIIDNGGID
Sbjct: 181 SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID 240

Query: 241 TDQDYPYKGRDGACDPNR----VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF 300
           TD+DYPYKGRDGACDPNR    VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF
Sbjct: 241 TDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF 300

Query: 301 QLYQSGVFTGRCGTNLDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANIT 360
           QLYQSGVFTGRCGTNLDHGVV VGYGT+NGTDYWIVRNSWGKDWGENGYIRLERNVAN T
Sbjct: 301 QLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANST 360

Query: 361 TGKCGIAVEPSYPIKTGSNPPKPSASPPSPVKPPTECDEYFS---------LWKH----L 420
           TGKCGIAVEPSYPIK+GSNPPKPSASPPSPV PPTECDEYFS         ++++     
Sbjct: 361 TGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF 420

Query: 421 LWLGMLPLESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAKQTKRIQ 453
            W G  PLESATCCDDHYSCCPHEYPVCDLEAGTCR SKDS MGVNLLKRLPA QTKRIQ
Sbjct: 421 AW-GCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQ 480

BLAST of PI0004966 vs. ExPASy TrEMBL
Match: A0A6J1D149 (low-temperature-induced cysteine proteinase-like OS=Momordica charantia OX=3673 GN=LOC111016644 PE=3 SV=1)

HSP 1 Score: 734.2 bits (1894), Expect = 3.3e-208
Identity = 363/474 (76.58%), Postives = 400/474 (84.39%), Query Frame = 0

Query: 9   AFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQ---RTDEEVAALYESW 68
           A LALFLC   FSSAS SS+FSIIDENAKHHLG P+I  SDA     RT E+VAALY+SW
Sbjct: 13  ASLALFLCFLSFSSASDSSSFSIIDENAKHHLGFPDIAGSDAGTPPLRTQEQVAALYKSW 72

Query: 69  LVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRETRTYKVGLTRFADLTNEEYRASL--- 128
           LV HGKAYNA+GE+ERRFEIFKDNLRFIDEHNRE R+Y +GLTRFADLTNEEYRA     
Sbjct: 73  LVKHGKAYNALGERERRFEIFKDNLRFIDEHNREPRSYTLGLTRFADLTNEEYRARFLGG 132

Query: 129 --------SAPKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSTVAAVEGI 188
                   SA K+GRYA++LG DLP+ VDWR+KGAV  VKDQGQCGSCWAFST+AAVEGI
Sbjct: 133 RFSPKPRPSAAKNGRYASSLGGDLPEHVDWREKGAVTAVKDQGQCGSCWAFSTIAAVEGI 192

Query: 189 NQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTDQDYPYKGRDGAC 248
           NQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFI+DNGGIDT++DYPYKGRDG C
Sbjct: 193 NQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIVDNGGIDTEEDYPYKGRDGVC 252

Query: 249 DPNR----VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGT 308
           DPNR    VVTIDGYEDVPENDE +LKKAVA+QPVSVAIEAGGRAFQLYQSGVFTGRCGT
Sbjct: 253 DPNRRNANVVTIDGYEDVPENDEGALKKAVASQPVSVAIEAGGRAFQLYQSGVFTGRCGT 312

Query: 309 NLDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANITTGKCGIAVEPSYPI 368
           +LDHGVV VGYGTENG DYWIVRNSWGK+WGENGYIRLERNVANITTGKCGIA+EPSYP+
Sbjct: 313 DLDHGVVAVGYGTENGLDYWIVRNSWGKNWGENGYIRLERNVANITTGKCGIAIEPSYPV 372

Query: 369 KTGSNPPKPSASPPSPVKPPTECDEYFS---------LWKH---LLWLGMLPLESATCCD 428
           KTG NPPKP+ SPPSPVKPPTECD+Y+S         ++++       G  PLESATCCD
Sbjct: 373 KTGKNPPKPAPSPPSPVKPPTECDDYYSCPEGTTCCCIYQYGSTCFGWGCCPLESATCCD 432

Query: 429 DHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAKQTKRIQKLGKLFVGA 453
           D YSCCP EYPVCDL  GTCR+SK S +GV+LLKRLPAK    +QKLGK+ +G+
Sbjct: 433 DQYSCCPREYPVCDLAEGTCRMSKGSLIGVSLLKRLPAKHKGVVQKLGKMIIGS 486

BLAST of PI0004966 vs. ExPASy TrEMBL
Match: A0A6J1JKG6 (low-temperature-induced cysteine proteinase-like OS=Cucurbita maxima OX=3661 GN=LOC111486001 PE=3 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 2.9e-204
Identity = 368/485 (75.88%), Postives = 396/485 (81.65%), Query Frame = 0

Query: 1   MAISPIF----FAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIP-EIPHSDAHQRTD 60
           MAIS  F     AFLALFLC S  S AS SS+FSIIDENAKHHL +    PH     R+D
Sbjct: 1   MAISTTFSFSIVAFLALFLCFSSSSLASDSSSFSIIDENAKHHLDVQIGSPH-----RSD 60

Query: 61  EEVAALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRETRTYKVGLTRFADLTN 120
           EEVAALYESWLVH+GKAYNA+GEKERRFEIFKDNLRFIDEHNRE+R+YKVGL RFADL+N
Sbjct: 61  EEVAALYESWLVHNGKAYNALGEKERRFEIFKDNLRFIDEHNRESRSYKVGLNRFADLSN 120

Query: 121 EEYRA-----------SLSAPKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWA 180
           EEYRA            LSA KS RYA  LGDDLPD VDWRKKGAV  VKDQGQCGSCWA
Sbjct: 121 EEYRAMFLSGRLSRKNRLSATKSRRYAITLGDDLPDHVDWRKKGAVTDVKDQGQCGSCWA 180

Query: 181 FSTVAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTDQD 240
           FSTVAAVEGINQI TGELISLSEQELVDCD+SYNMGCNGGLMDYAFQFIIDNGGIDT++D
Sbjct: 181 FSTVAAVEGINQITTGELISLSEQELVDCDRSYNMGCNGGLMDYAFQFIIDNGGIDTEED 240

Query: 241 YPYKGRDGACDPNR----VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQ 300
           YPYKGRDG CDPNR     VTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQ
Sbjct: 241 YPYKGRDGTCDPNRRNSKAVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQ 300

Query: 301 SGVFTGRCGTNLDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANITTGKC 360
           SGVF G CGT LDHGVV VGYGTE+G DYWIVRNSWGK+WGENGYI+LERNV NITTGKC
Sbjct: 301 SGVFNGLCGTELDHGVVAVGYGTEDGKDYWIVRNSWGKNWGENGYIKLERNVGNITTGKC 360

Query: 361 GIAVEPSYPIKTGSNPPKPSASPPSPVKPPTECDEYFS---------LWKH---LLWLGM 420
           GIA+E SYP KTG NPPKP+ SPPSPV PP +CDEYFS         ++K+       G 
Sbjct: 361 GIAIEASYPTKTGKNPPKPAPSPPSPVSPPNQCDEYFSCQEGTTCCCIYKYGSTCFGWGC 420

Query: 421 LPLESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAKQTKR-IQKLGK 453
            PLESATCCDDHYSCCPHEYP+CDL+AGTC  SK S+ GV LLKR+PAK  +  +QKLGK
Sbjct: 421 CPLESATCCDDHYSCCPHEYPICDLDAGTCLKSKGSTTGVKLLKRVPAKYNRGCVQKLGK 480

BLAST of PI0004966 vs. NCBI nr
Match: XP_008454976.1 (PREDICTED: low-temperature-induced cysteine proteinase-like [Cucumis melo])

HSP 1 Score: 853.6 bits (2204), Expect = 7.8e-244
Identity = 428/481 (88.98%), Postives = 438/481 (91.06%), Query Frame = 0

Query: 1   MAI-SPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60
           MAI SPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA
Sbjct: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60

Query: 61  ALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRETRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNA+GEKERRFEIFKDNL FIDEHNRE+RTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 A-----------SLSAPKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSTV 180
           A           SLSA KSGRYAAALGDDLPDDVDWRKKGAVA VKDQGQCGSCWAFSTV
Sbjct: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQCGSCWAFSTV 180

Query: 181 AAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTDQDYPYK 240
           AAVEGINQIVTGELISLSEQELVDCDKS+NMGCNGGLMDYAFQFIIDNGGIDTD+DYPYK
Sbjct: 181 AAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGIDTDEDYPYK 240

Query: 241 GRDGACDPNR----VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300
           GRDGACDPNR    VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF
Sbjct: 241 GRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVF 300

Query: 301 TGRCGTNLDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANITTGKCGIAV 360
           TGRCGTNLDHGVV VGYGT+NGTDYWIVRNSWGKDWGENGYIRLERNVAN TTGKCGIAV
Sbjct: 301 TGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANSTTGKCGIAV 360

Query: 361 EPSYPIKTGSNPPKPSASPPSPVKPPTECDEYFS---------LWKH----LLWLGMLPL 420
           EPSYPIK+GSNPPKPSASPPSPV PPTECDEYFS         ++++      W G  PL
Sbjct: 361 EPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCFAW-GCCPL 420

Query: 421 ESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAKQTKRIQKLGKLFVG 453
           ESATCCDDHYSCCPHEYPVCDLEAGTCR SKDS MGVNLLKRLPA QTKRIQKLGKLFVG
Sbjct: 421 ESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQKLGKLFVG 480

BLAST of PI0004966 vs. NCBI nr
Match: XP_004136967.1 (low-temperature-induced cysteine proteinase [Cucumis sativus] >KGN43904.1 hypothetical protein Csa_017340 [Cucumis sativus])

HSP 1 Score: 844.3 bits (2180), Expect = 4.7e-241
Identity = 420/480 (87.50%), Postives = 434/480 (90.42%), Query Frame = 0

Query: 1   MAISPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAA 60
           MAISPIFFAFLALF CLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQR DEEVAA
Sbjct: 1   MAISPIFFAFLALFFCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRPDEEVAA 60

Query: 61  LYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRETRTYKVGLTRFADLTNEEYRA 120
           LYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRE+RTYKVGLTRFADLTNEEYRA
Sbjct: 61  LYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADLTNEEYRA 120

Query: 121 -----------SLSAPKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSTVA 180
                       LSA KSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFS+VA
Sbjct: 121 RFLGGRFSRKPRLSAAKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSSVA 180

Query: 181 AVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTDQDYPYKG 240
           AVEGINQIVTGELI LSEQELVDCDKS+NMGCNGGLMDYAFQFII NGGIDT++DYPYKG
Sbjct: 181 AVEGINQIVTGELIPLSEQELVDCDKSFNMGCNGGLMDYAFQFIIGNGGIDTEEDYPYKG 240

Query: 241 RDGACDPNR----VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFT 300
           RD ACDPNR    VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFT
Sbjct: 241 RDAACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFT 300

Query: 301 GRCGTNLDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANITTGKCGIAVE 360
           GRCGT+LDHGVV VGYGT+NGTDYWIVRNSWGKDWGE+GYIRLERNVANITTGKCGIAV+
Sbjct: 301 GRCGTDLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGESGYIRLERNVANITTGKCGIAVQ 360

Query: 361 PSYPIKTGSNPPKPSASPPSPVKPPTECDEYFSLWKH-------------LLWLGMLPLE 420
           PSYP K+G+NPPKPSASPPSPVKPPTECDEYFS  +                W G  PLE
Sbjct: 361 PSYPTKSGANPPKPSASPPSPVKPPTECDEYFSCEEGSTCCCIYQFGSTCFAW-GCCPLE 420

Query: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAKQTKRIQKLGKLFVGA 453
           SATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPA QTK++QKLGKLFVGA
Sbjct: 421 SATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAIQTKKVQKLGKLFVGA 479

BLAST of PI0004966 vs. NCBI nr
Match: KAA0031322.1 (low-temperature-induced cysteine proteinase-like [Cucumis melo var. makuwa] >TYK06773.1 low-temperature-induced cysteine proteinase-like [Cucumis melo var. makuwa])

HSP 1 Score: 842.4 bits (2175), Expect = 1.8e-240
Identity = 427/489 (87.32%), Postives = 437/489 (89.37%), Query Frame = 0

Query: 1   MAI-SPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60
           MAI SPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA
Sbjct: 1   MAISSPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVA 60

Query: 61  ALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRETRTYKVGLTRFADLTNEEYR 120
           ALYESWLVHHGKAYNA+GEKERRFEIFKDNL FIDEHNRE+RTYKVGLTRFADLTNEEYR
Sbjct: 61  ALYESWLVHHGKAYNALGEKERRFEIFKDNLMFIDEHNRESRTYKVGLTRFADLTNEEYR 120

Query: 121 A-----------SLSAPKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQ--------CG 180
           A           SLSA KSGRYAAALGDDLPDDVDWRKKGAVA VKDQGQ         G
Sbjct: 121 ARFLGGRFSRKPSLSAAKSGRYAAALGDDLPDDVDWRKKGAVANVKDQGQKFVMRSIFIG 180

Query: 181 SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGID 240
           SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKS+NMGCNGGLMDYAFQFIIDNGGID
Sbjct: 181 SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSFNMGCNGGLMDYAFQFIIDNGGID 240

Query: 241 TDQDYPYKGRDGACDPNR----VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF 300
           TD+DYPYKGRDGACDPNR    VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF
Sbjct: 241 TDEDYPYKGRDGACDPNRKNAKVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF 300

Query: 301 QLYQSGVFTGRCGTNLDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANIT 360
           QLYQSGVFTGRCGTNLDHGVV VGYGT+NGTDYWIVRNSWGKDWGENGYIRLERNVAN T
Sbjct: 301 QLYQSGVFTGRCGTNLDHGVVAVGYGTDNGTDYWIVRNSWGKDWGENGYIRLERNVANST 360

Query: 361 TGKCGIAVEPSYPIKTGSNPPKPSASPPSPVKPPTECDEYFS---------LWKH----L 420
           TGKCGIAVEPSYPIK+GSNPPKPSASPPSPV PPTECDEYFS         ++++     
Sbjct: 361 TGKCGIAVEPSYPIKSGSNPPKPSASPPSPVNPPTECDEYFSCDEGSTCCCIYQYGSTCF 420

Query: 421 LWLGMLPLESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAKQTKRIQ 453
            W G  PLESATCCDDHYSCCPHEYPVCDLEAGTCR SKDS MGVNLLKRLPA QTKRIQ
Sbjct: 421 AW-GCCPLESATCCDDHYSCCPHEYPVCDLEAGTCRASKDSLMGVNLLKRLPANQTKRIQ 480

BLAST of PI0004966 vs. NCBI nr
Match: XP_038889032.1 (low-temperature-induced cysteine proteinase-like [Benincasa hispida])

HSP 1 Score: 800.0 bits (2065), Expect = 1.0e-227
Identity = 402/486 (82.72%), Postives = 426/486 (87.65%), Query Frame = 0

Query: 1   MAISPIF----FAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSD---AHQR 60
           MAIS  F     AFLALFLC SPFSSASHSSTFSIIDENAKHH+GIP+I  SD   + QR
Sbjct: 1   MAISSTFSFLTLAFLALFLCFSPFSSASHSSTFSIIDENAKHHMGIPDIADSDHLRSPQR 60

Query: 61  TDEEVAALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRETRTYKVGLTRFADL 120
           TD+EVAALYESWLVHH KAYNA+GEKERRFEIFKDNLRFIDEHNRE+RTYKVGLTRFADL
Sbjct: 61  TDDEVAALYESWLVHHRKAYNALGEKERRFEIFKDNLRFIDEHNRESRTYKVGLTRFADL 120

Query: 121 TNEEYRA-----------SLSAPKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSC 180
           TNEEYRA            LSA KSGRYAAALGDDLPD VDWRKKGAVA VKDQGQCGSC
Sbjct: 121 TNEEYRARFLGGRFPGKPRLSAAKSGRYAAALGDDLPDHVDWRKKGAVAAVKDQGQCGSC 180

Query: 181 WAFSTVAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTD 240
           WAFSTVAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDT+
Sbjct: 181 WAFSTVAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTE 240

Query: 241 QDYPYKGRDGACDPNR----VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQL 300
           +DYPYKG D ACDPNR    VVTIDG+EDVPENDESSLKKAVANQPVSVAIEAGGRAFQL
Sbjct: 241 EDYPYKGHDAACDPNRKNAKVVTIDGFEDVPENDESSLKKAVANQPVSVAIEAGGRAFQL 300

Query: 301 YQSGVFTGRCGTNLDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANITTG 360
           YQSGVFTGRCGTNLDHGVV VGYGTENGTDYWIVRNSWGK+WGE+GYIRLERNVANITTG
Sbjct: 301 YQSGVFTGRCGTNLDHGVVAVGYGTENGTDYWIVRNSWGKNWGESGYIRLERNVANITTG 360

Query: 361 KCGIAVEPSYPIKTGSNPPKPSASPPSPVKPPTECDEYFS---------LWKH---LLWL 420
           KCGIAV+PSYPIKTGSNPPKP+ASPPSPVKPPTECD+Y+S         ++++       
Sbjct: 361 KCGIAVQPSYPIKTGSNPPKPAASPPSPVKPPTECDDYYSCEEGTTCCCIYQYGSTCFGW 420

Query: 421 GMLPLESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAKQTKRIQKLG 453
           G  PLESATCCDDHYSCCPHEYP+CDLEAGTC + KDS+MGV LLKRLPAK+TK IQK G
Sbjct: 421 GCCPLESATCCDDHYSCCPHEYPICDLEAGTCLIDKDSTMGVKLLKRLPAKRTKGIQKFG 480

BLAST of PI0004966 vs. NCBI nr
Match: XP_022147795.1 (low-temperature-induced cysteine proteinase-like [Momordica charantia])

HSP 1 Score: 734.2 bits (1894), Expect = 6.9e-208
Identity = 363/474 (76.58%), Postives = 400/474 (84.39%), Query Frame = 0

Query: 9   AFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQ---RTDEEVAALYESW 68
           A LALFLC   FSSAS SS+FSIIDENAKHHLG P+I  SDA     RT E+VAALY+SW
Sbjct: 13  ASLALFLCFLSFSSASDSSSFSIIDENAKHHLGFPDIAGSDAGTPPLRTQEQVAALYKSW 72

Query: 69  LVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNRETRTYKVGLTRFADLTNEEYRASL--- 128
           LV HGKAYNA+GE+ERRFEIFKDNLRFIDEHNRE R+Y +GLTRFADLTNEEYRA     
Sbjct: 73  LVKHGKAYNALGERERRFEIFKDNLRFIDEHNREPRSYTLGLTRFADLTNEEYRARFLGG 132

Query: 129 --------SAPKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSTVAAVEGI 188
                   SA K+GRYA++LG DLP+ VDWR+KGAV  VKDQGQCGSCWAFST+AAVEGI
Sbjct: 133 RFSPKPRPSAAKNGRYASSLGGDLPEHVDWREKGAVTAVKDQGQCGSCWAFSTIAAVEGI 192

Query: 189 NQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTDQDYPYKGRDGAC 248
           NQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFI+DNGGIDT++DYPYKGRDG C
Sbjct: 193 NQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIVDNGGIDTEEDYPYKGRDGVC 252

Query: 249 DPNR----VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGT 308
           DPNR    VVTIDGYEDVPENDE +LKKAVA+QPVSVAIEAGGRAFQLYQSGVFTGRCGT
Sbjct: 253 DPNRRNANVVTIDGYEDVPENDEGALKKAVASQPVSVAIEAGGRAFQLYQSGVFTGRCGT 312

Query: 309 NLDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANITTGKCGIAVEPSYPI 368
           +LDHGVV VGYGTENG DYWIVRNSWGK+WGENGYIRLERNVANITTGKCGIA+EPSYP+
Sbjct: 313 DLDHGVVAVGYGTENGLDYWIVRNSWGKNWGENGYIRLERNVANITTGKCGIAIEPSYPV 372

Query: 369 KTGSNPPKPSASPPSPVKPPTECDEYFS---------LWKH---LLWLGMLPLESATCCD 428
           KTG NPPKP+ SPPSPVKPPTECD+Y+S         ++++       G  PLESATCCD
Sbjct: 373 KTGKNPPKPAPSPPSPVKPPTECDDYYSCPEGTTCCCIYQYGSTCFGWGCCPLESATCCD 432

Query: 429 DHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAKQTKRIQKLGKLFVGA 453
           D YSCCP EYPVCDL  GTCR+SK S +GV+LLKRLPAK    +QKLGK+ +G+
Sbjct: 433 DQYSCCPREYPVCDLAEGTCRMSKGSLIGVSLLKRLPAKHKGVVQKLGKMIIGS 486

BLAST of PI0004966 vs. TAIR 10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 574.3 bits (1479), Expect = 8.5e-164
Identity = 288/451 (63.86%), Postives = 335/451 (74.28%), Query Frame = 0

Query: 13  LFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAALYESWLVHHGKA 72
           LFL +   SSA      SII  + KH +       S    R++ EV ++YE+WLV HGKA
Sbjct: 11  LFLAMVAVSSA---VDMSIISYDEKHGV-------STTGGRSEAEVMSIYEAWLVKHGKA 70

Query: 73  --YNAIGEKERRFEIFKDNLRFIDEHNRETRTYKVGLTRFADLTNEEYRASLSAPK---- 132
              N++ EK+RRFEIFKDNLRF+DEHN +  +Y++GLTRFADLTN+EYR+     K    
Sbjct: 71  QSQNSLVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKK 130

Query: 133 -----SGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSTVAAVEGINQIVTGE 192
                S RY A +GD+LP+ +DWRKKGAVA VKDQG CGSCWAFST+ AVEGINQIVTG+
Sbjct: 131 GERRTSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGD 190

Query: 193 LISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTDQDYPYKGRDGACDPNR--- 252
           LI+LSEQELVDCD SYN GCNGGLMDYAF+FII NGGIDTD+DYPYKG DG CD  R   
Sbjct: 191 LITLSEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNA 250

Query: 253 -VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGTNLDHGVV 312
            VVTID YEDVP   E SLKKAVA+QP+S+AIEAGGRAFQLY SG+F G CGT LDHGVV
Sbjct: 251 KVVTIDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHGVV 310

Query: 313 TVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANITTGKCGIAVEPSYPIKTGSNPP 372
            VGYGTENG DYWIVRNSWGK WGE+GY+R+ RN+A+ ++GKCGIA+EPSYPIK G NPP
Sbjct: 311 AVGYGTENGKDYWIVRNSWGKSWGESGYLRMARNIAS-SSGKCGIAIEPSYPIKNGENPP 370

Query: 373 KPSASPPSPVKPPTECDEY------------FSLWKHLLWLGMLPLESATCCDDHYSCCP 432
            P  SPPSP+KPPT+CD Y            F   K+    G  PLE+ATCCDD+YSCCP
Sbjct: 371 NPGPSPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNYSCCP 430

Query: 433 HEYPVCDLEAGTCRVSKDSSMGVNLLKRLPA 437
           HEYPVCDL+ GTC +SK+S   V  LKR PA
Sbjct: 431 HEYPVCDLDQGTCLLSKNSPFSVKALKRKPA 450

BLAST of PI0004966 vs. TAIR 10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 565.5 bits (1456), Expect = 3.9e-161
Identity = 287/457 (62.80%), Postives = 329/457 (71.99%), Query Frame = 0

Query: 8   FAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAALYESWLV 67
           F  L+  + L      S++   SII  +  HH+       +    R+D EV  +YE+W+V
Sbjct: 3   FLKLSPMILLLAMIGVSYAMDMSIISYDENHHI-------TTETSRSDSEVERIYEAWMV 62

Query: 68  HHGKA---YNAIG-EKERRFEIFKDNLRFIDEHNRETRTYKVGLTRFADLTNEEYRASLS 127
            HGK     N +G EK++RFEIFKDNLRFIDEHN +  +YK+GLTRFADLTNEEYR+   
Sbjct: 63  EHGKKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYL 122

Query: 128 APK--------SGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGSCWAFSTVAAVEGIN 187
             K        S RY A +GD LPD VDWRK+GAVA VKDQG CGSCWAFST+ AVEGIN
Sbjct: 123 GAKPTKRVLKTSDRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGIN 182

Query: 188 QIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTDQDYPYKGRDGACD 247
           +IVTG+LISLSEQELVDCD SYN GCNGGLMDYAF+FII NGGIDT+ DYPYK  DG CD
Sbjct: 183 KIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCD 242

Query: 248 PNR----VVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQLYQSGVFTGRCGTN 307
            NR    VVTID YEDVPEN E+SLKKA+A+QP+SVAIEAGGRAFQLY SGVF G CGT 
Sbjct: 243 QNRKNAKVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTE 302

Query: 308 LDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANITTGKCGIAVEPSYPIK 367
           LDHGVV VGYGTENG DYWIVRNSWG  WGE+GYI++ RN+    TGKCGIA+E SYPIK
Sbjct: 303 LDHGVVAVGYGTENGKDYWIVRNSWGNRWGESGYIKMARNI-EAPTGKCGIAMEASYPIK 362

Query: 368 TGSNPPKPSASPPSPVKPPTECDEYFS------------LWKHLLWLGMLPLESATCCDD 427
            G NPP P  SPPSP+KPPT CD+YFS              K+    G  PLE+ATCCDD
Sbjct: 363 KGQNPPNPGPSPPSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAATCCDD 422

Query: 428 HYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPA 437
           + SCCPHEYPVCD+  GTC +SK+S   V  LKR PA
Sbjct: 423 NSSCCPHEYPVCDVNRGTCLMSKNSPFSVKALKRTPA 451

BLAST of PI0004966 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 497.3 bits (1279), Expect = 1.3e-140
Identity = 258/414 (62.32%), Postives = 298/414 (71.98%), Query Frame = 0

Query: 53  RTDEEVAALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNR-ETRTYKVGLTRFA 112
           R + E   +YE WLV + K YN +GEKERRFEIFKDNL+F++EH+    RTY+VGLTRFA
Sbjct: 34  RNEAEARRMYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEEHSSIPNRTYEVGLTRFA 93

Query: 113 DLTNEEYRASLSAPKSGR---------YAAALGDDLPDDVDWRKKGAVATVKDQGQCGSC 172
           DLTN+E+RA     K  R         Y   +GD LPD +DWR KGAV  VKDQG CGSC
Sbjct: 94  DLTNDEFRAIYLRSKMERTRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSC 153

Query: 173 WAFSTVAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGIDTD 232
           WAFS + AVEGINQI TGELISLSEQELVDCD SYN GC GGLMDYAF+FII+NGGIDT+
Sbjct: 154 WAFSAIGAVEGINQIKTGELISLSEQELVDCDTSYNDGCGGGLMDYAFKFIIENGGIDTE 213

Query: 233 QDYPYKGRD-GACDPN----RVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAFQ 292
           +DYPY   D   C+ +    RVVTIDGYEDVP+NDE SLKKA+ANQP+SVAIEAGGRAFQ
Sbjct: 214 EDYPYIATDVNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQ 273

Query: 293 LYQSGVFTGRCGTNLDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANITT 352
           LY SGVFTG CGT+LDHGVV VGYG+E G DYWIVRNSWG +WGE+GY +LERN+   ++
Sbjct: 274 LYTSGVFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRNSWGSNWGESGYFKLERNIKE-SS 333

Query: 353 GKCGIAVEPSYPIK-TGSNPPKPSASPPSPV---------KPPTECDEYFSLWKHLLWLG 412
           GKCG+A+  SYP K +GSNPPKP A  PSPV            T C  Y    K   W G
Sbjct: 334 GKCGVAMMASYPTKSSGSNPPKPPA--PSPVVCDKSNTCPAKSTCCCLYEYNGKCYSW-G 393

Query: 413 MLPLESATCCDDHYSCCPHEYPVCDLEAGTCRVSKDSSMGVNLLKRLPAKQTKR 442
             P ESATCCDD  SCCP  YPVCDL+A TCR+  +S + +  L R PA  T +
Sbjct: 394 CCPYESATCCDDGSSCCPQSYPVCDLKANTCRMKGNSPLSIKALTRGPAIATTK 443

BLAST of PI0004966 vs. TAIR 10
Match: AT4G36880.1 (cysteine proteinase1 )

HSP 1 Score: 425.6 bits (1093), Expect = 4.9e-119
Identity = 225/380 (59.21%), Postives = 272/380 (71.58%), Query Frame = 0

Query: 1   MAISPIFFAFLALFLCLSPFSSASHSSTFSIIDENAKHHLGIPEIPHSDAHQRTDEEVAA 60
           MA S    + L L++ +S  S        SII++    HL +P    SD   RTDEEV +
Sbjct: 1   MAPSTKVLSLLLLYVVVSLASGDE-----SIIND----HLQLP----SDGKWRTDEEVRS 60

Query: 61  LYESWLVHHGKAYN----AIGEKERRFEIFKDNLRFIDEHNRETR--TYKVGLTRFADLT 120
           +Y  W   HGK  N     I ++++RF IFKDNLRFID HN   +  TYK+GLT+F DLT
Sbjct: 61  IYLQWSAEHGKTNNNNNGIINDQDKRFNIFKDNLRFIDLHNENNKNATYKLGLTKFTDLT 120

Query: 121 NEEYRASLSAPK-------------SGRYAAAL-GDDLPDDVDWRKKGAVATVKDQGQCG 180
           N+EYR      +             + +Y+AA+ G ++P+ VDWR+KGAV  +KDQG CG
Sbjct: 121 NDEYRKLYLGARTEPARRIAKAKNVNQKYSAAVNGKEVPETVDWRQKGAVNPIKDQGTCG 180

Query: 181 SCWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSYNMGCNGGLMDYAFQFIIDNGGID 240
           SCWAFST AAVEGIN+IVTGELISLSEQELVDCDKSYN GCNGGLMDYAFQFI+ NGG++
Sbjct: 181 SCWAFSTTAAVEGINKIVTGELISLSEQELVDCDKSYNQGCNGGLMDYAFQFIMKNGGLN 240

Query: 241 TDQDYPYKGRDGACDP----NRVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGRAF 300
           T++DYPY+G  G C+     +RVV+IDGYEDVP  DE++LKKA++ QPVSVAIEAGGR F
Sbjct: 241 TEKDYPYRGFGGKCNSFLKNSRVVSIDGYEDVPTKDETALKKAISYQPVSVAIEAGGRIF 300

Query: 301 QLYQSGVFTGRCGTNLDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVANIT 357
           Q YQSG+FTG CGTNLDH VV VGYG+ENG DYWIVRNSWG  WGE GYIR+ERN+A   
Sbjct: 301 QHYQSGIFTGSCGTNLDHAVVAVGYGSENGVDYWIVRNSWGPRWGEEGYIRMERNLAASK 360

BLAST of PI0004966 vs. TAIR 10
Match: AT3G19400.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 412.5 bits (1059), Expect = 4.3e-115
Identity = 204/318 (64.15%), Postives = 248/318 (77.99%), Query Frame = 0

Query: 52  QRTDEEVAALYESWLVHHGKAYNAIGEKERRFEIFKDNLRFIDEHNR-ETRTYKVGLTRF 111
           +R + EV  +YE WLV + K YN +GEKERRF+IFKDNL+F+DEHN    RT++VGLTRF
Sbjct: 34  ERNETEVRLMYEQWLVENRKNYNGLGEKERRFKIFKDNLKFVDEHNSVPDRTFEVGLTRF 93

Query: 112 ADLTNEEYRA---------SLSAPKSGRYAAALGDDLPDDVDWRKKGAVATVKDQGQCGS 171
           ADLTNEE+RA         +  + K+ RY    GD LPD+VDWR  GAV +VKDQG CGS
Sbjct: 94  ADLTNEEFRAIYLRKKMERTKDSVKTERYLYKEGDVLPDEVDWRANGAVVSVKDQGNCGS 153

Query: 172 CWAFSTVAAVEGINQIVTGELISLSEQELVDCDKSY-NMGCNGGLMDYAFQFIIDNGGID 231
           CWAFS V AVEGINQI TGELISLSEQELVDCD+ + N GC+GG+M+YAF+FI+ NGGI+
Sbjct: 154 CWAFSAVGAVEGINQITTGELISLSEQELVDCDRGFVNAGCDGGIMNYAFEFIMKNGGIE 213

Query: 232 TDQDYPYKGRD-GACDPN-----RVVTIDGYEDVPENDESSLKKAVANQPVSVAIEAGGR 291
           TDQDYPY   D G C+ +     RVVTIDGYEDVP +DE SLKKAVA+QPVSVAIEA  +
Sbjct: 214 TDQDYPYNANDLGLCNADKNNNTRVVTIDGYEDVPRDDEKSLKKAVAHQPVSVAIEASSQ 273

Query: 292 AFQLYQSGVFTGRCGTNLDHGVVTVGYGTENGTDYWIVRNSWGKDWGENGYIRLERNVAN 351
           AFQLY+SGV TG CG +LDHGVV VGYG+ +G DYWI+RNSWG +WG++GY++L+RN+ +
Sbjct: 274 AFQLYKSGVMTGTCGISLDHGVVVVGYGSTSGEDYWIIRNSWGLNWGDSGYVKLQRNIDD 333

Query: 352 ITTGKCGIAVEPSYPIKT 353
              GKCGIA+ PSYP K+
Sbjct: 334 -PFGKCGIAMMPSYPTKS 350