PI0004915 (gene) Melon (PI 482460) v1

BLAST of PI0004915 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 8.7e-195
Identity = 354/564 (62.77%), Postives = 431/564 (76.42%), Query Frame = 0

Query: 6   RKIIGARTYR-SENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVP 65
           RKIIGAR+Y     + PG++  PRD+ GHGTHTAST  G LVSQA+LYGLG GTARGGVP
Sbjct: 176 RKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVP 235

Query: 66  SARIAVYKICWSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHAM 125
            ARIA YK+CW+DGC D+DILAA+DDAIADGVDIISLSVGG+  + YF D+IAIG+FHA+
Sbjct: 236 LARIAAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAV 295

Query: 126 KHGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHTFD 185
           + GILTSNSAGN GP +FTT+++SPW LSVAAST+DRKFV++VQ+ NG   QG +I+TFD
Sbjct: 296 ERGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD 355

Query: 186 LMGKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESFNK 245
              + YPL+ G D PN   GF+ S SRFC + SV+P+L+KGKI+VC++       E F  
Sbjct: 356 --NQYYPLVSGRDIPNT--GFDKSTSRFCTDKSVNPNLLKGKIVVCEASF--GPHEFFKS 415

Query: 246 -NGAVGIIMQGSRFKDYASSYPLPASYLHNANI---------THSSTSTIFKSNAILDAS 305
            +GA G++M  S  +DYA SYPLP+S L   ++           S  +TIFKS  IL+AS
Sbjct: 416 LDGAAGVLMT-SNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNAS 475

Query: 306 APSVVSFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGTS 365
           AP VVSFSSRGPN AT D++KPD++ PGVEILAAW  +APV G+    R+ L+NIISGTS
Sbjct: 476 APVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGI---RRNTLFNIISGTS 535

Query: 366 MSCPHATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKALN 425
           MSCPH T IA YVKT+NPTWSPAAIKSALMTTA  MNA+ NPQAEFAYG+GH+NPLKA+ 
Sbjct: 536 MSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVR 595

Query: 426 PGLVYNATETDYINFLCGQEGYTTDMVRRITGDKTACTPANSGRVWDLNYPSFAFSTTPS 485
           PGLVY+A E+DY+ FLCGQ GY T  VRRITGD +ACT  N+GRVWDLNYPSF  S +PS
Sbjct: 596 PGLVYDANESDYVKFLCGQ-GYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPS 655

Query: 486 QLIINQFFTRTLTNVEFKASLYSAKVFAPPNLRITVDPPSLLFNGIGDTKSFKLTVQGTV 545
           Q   NQ+F RTLT+V  +AS Y A + AP  L I+V+P  L FNG+GD KSF LTV+G++
Sbjct: 656 Q-TFNQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSI 715

Query: 546 NQNIVSGSLVWTDGVHQVRSPITV 559
              +VS SLVW+DGVH VRSPIT+
Sbjct: 716 KGFVVSASLVWSDGVHYVRSPITI 727

BLAST of PI0004915 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 4.4e-130
Identity = 269/576 (46.70%), Postives = 357/576 (61.98%), Query Frame = 0

Query: 7   KIIGARTYRSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVPSA 66
           K+IGAR Y           S RD EGHGTHTAST  G+ V  AS YGL  GTARGGVPSA
Sbjct: 176 KLIGARFYNK------FADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSA 235

Query: 67  RIAVYKICWSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHAMKH 126
           RIA YK+C+ + C D DILAAFDDAIADGVD+IS+S+    V +    S+AIG+FHAM  
Sbjct: 236 RIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMR 295

Query: 127 GILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHTFDLM 186
           GI+T+ SAGN GP+  + +NVSPW ++VAAS  DR+F+ RV L NG    G +++TF+L 
Sbjct: 296 GIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLN 355

Query: 187 GKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESFNKNG 246
           G ++P++YG    N S   + + + +C    VD  LVKGKI++CD  L     E++   G
Sbjct: 356 GTKFPIVYG---QNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFL--GYREAY-LAG 415

Query: 247 AVGIIMQGSRFKDYASSYPLPA------------SYLHNANITHSSTSTIFKSNAILDAS 306
           A+G+I+Q +   D A   P PA            SY+ +A       + I ++  I+D  
Sbjct: 416 AIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESA---EPPQAEILRTEEIVDRE 475

Query: 307 APSVVSFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGV--AEDSRSVLYNIISG 366
           AP V SFSSRGP+    ++LKPD++APG+EILAA+SP+A  S     ED RSV Y+++SG
Sbjct: 476 APYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSG 535

Query: 367 TSMSCPHATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKA 426
           TSM+CPH   +A YVK+F+P WSP+AIKSA+MTTA  MN K NP+ EFAYG+G INP KA
Sbjct: 536 TSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKA 595

Query: 427 LNPGLVYNATETDYINFLCGQEGYTTDMVRRITGDKTACTPANSGRVWDLNYP---SFAF 486
            +PGLVY     DY+  LC  EG+ +  +   +G    C+      V DLNYP   +F  
Sbjct: 596 SDPGLVYEVETEDYLKMLCA-EGFDSTTLTTTSGQNVTCSERT--EVKDLNYPTMTTFVS 655

Query: 487 STTPSQLIINQFFTRTLTNVEFKASLYSAKVF-APPNLRITVDPPSLLFNGIGDTKSFKL 546
           S  P     N  F RT+TNV F  S Y A V    P L+I+++P  L F  + + KSF +
Sbjct: 656 SLDP----FNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVV 715

Query: 547 TVQG--TVNQNIVSGSLVWTDGVHQVRSPITVYVVK 563
           T+ G    + + VS S+VW+DG H VRSPI  Y ++
Sbjct: 716 TISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSIQ 728

BLAST of PI0004915 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 6.3e-129
Identity = 263/571 (46.06%), Postives = 356/571 (62.35%), Query Frame = 0

Query: 7   KIIGARTYRSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVPSA 66
           K+IGAR Y SE          RD +GHGTHTAST  G+ V  AS +G+G GT RGGVP++
Sbjct: 178 KLIGARDYTSE--------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPAS 237

Query: 67  RIAVYKICWSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHAMKH 126
           R+A YK+C   GC    +L+AFDDAIADGVD+I++S+G      +  D IAIGAFHAM  
Sbjct: 238 RVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAK 297

Query: 127 GILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHTFDLM 186
           G+LT NSAGN GP+  + S V+PW L+VAAST +R FV++V L NG    G +++ +++ 
Sbjct: 298 GVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMK 357

Query: 187 GKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESFNKNG 246
           GK YPL+YG  A   S   ++  +  C+ + VD S VKGKILVC        +ES    G
Sbjct: 358 GKDYPLVYGKSA--ASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV---G 417

Query: 247 AVGIIMQGSRFKDYASSYPLPA------------SYLHNANITHSSTSTIFKSNAILDAS 306
           AVG+I +  +  D A  +PLPA            SYL +   T S  + + K+ AI + +
Sbjct: 418 AVGLIYRTPK-PDVAFIHPLPAAGLLTEDFESLVSYLES---TDSPQAIVLKTEAIFNRT 477

Query: 307 APSVVSFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGTS 366
           +P + SFSSRGPN   +DILKPD+TAPGVEILAA+SP    S   +D+R V Y+++SGTS
Sbjct: 478 SPVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTS 537

Query: 367 MSCPHATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVN--PQAEFAYGAGHINPLKA 426
           MSCPH   +A YVKTFNP WSP+ I+SA+MTTA+ +NA        EFAYG+GH++P+ A
Sbjct: 538 MSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAA 597

Query: 427 LNPGLVYNATETDYINFLCGQEGYTTDMVRRITGDKTACTPANSGRVWDLNYPSFAFSTT 486
            NPGLVY   ++D+I FLCG   YT+ +++ I+G+   C+ A      +LNYPS +   +
Sbjct: 598 SNPGLVYELDKSDHIAFLCGM-NYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLS 657

Query: 487 PSQLIINQFFTRTLTNVEFKASLYSAKVFA--PPNLRITVDPPSLLFNGIGDTKSFKLTV 546
            S       F RTLTNV    S Y++KV A     L + + P  L F  + + +SF +TV
Sbjct: 658 GSGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTV 717

Query: 547 QGTVNQNIV--SGSLVWTDGVHQVRSPITVY 560
            G+   + V  S +L+W+DG H VRSPI VY
Sbjct: 718 TGSNLDSEVPSSANLIWSDGTHNVRSPIVVY 728

BLAST of PI0004915 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 1.0e-126
Identity = 260/571 (45.53%), Postives = 361/571 (63.22%), Query Frame = 0

Query: 7   KIIGARTYRSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVPSA 66
           K+IGAR Y +++      Q+ RD  GHGTHTAS   G+ V+ ++ YGLG GTARGGVP+A
Sbjct: 181 KVIGARDYTAKSKAN---QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAA 240

Query: 67  RIAVYKICWSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHAMKH 126
           RIAVYK+C ++GC    +++AFDDAIADGVD+IS+S+    +  +  D IAIGAFHAM  
Sbjct: 241 RIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAV 300

Query: 127 GILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHTFDLM 186
           G+LT N+AGN GP+  T ++ +PW  SVAAS  +R F+++V L +G +  G +++T+D+ 
Sbjct: 301 GVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMN 360

Query: 187 GKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESFNKNG 246
           G  YPL+YG  A   +   +   +R C+   +D  LVKGKI++CDS      +    K G
Sbjct: 361 GTNYPLVYGKSAALSTCSVDK--ARLCEPKCLDGKLVKGKIVLCDS---TKGLIEAQKLG 420

Query: 247 AVGIIMQGSRFKDYASSYPLPASYLHN---------ANITHSSTSTIFKSNAILDASAPS 306
           AVG I++     D A     P S+L N          N T +  +T+ KS  I +  AP 
Sbjct: 421 AVGSIVKNPE-PDRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPL 480

Query: 307 VVSFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGTSMSC 366
           V SFSSRGP+    DILKPD+TAPGVEILAA+SP +  +    D+R V Y+++SGTSM+C
Sbjct: 481 VASFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMAC 540

Query: 367 PHATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVN--PQAEFAYGAGHINPLKALNP 426
           PH   +A YVKTF+P WSP+ I+SA+MTTA+ MNA  +     EFAYG+GH++P+ A+NP
Sbjct: 541 PHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINP 600

Query: 427 GLVYNATETDYINFLCGQEGYTTDMVRRITGDKTACTPANSGRV-WDLNYPSFAFSTTPS 486
           GLVY  T+ D+INFLCG   YT+D +R I+GD + CT   S  +  +LNYP+ +   + +
Sbjct: 601 GLVYELTKADHINFLCGL-NYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGT 660

Query: 487 QLIINQFFTRTLTNVEFKASLYSAKV--FAPPNLRITVDPPSLLFNGIGDTKSFKLTVQG 546
           +   N  F RT+TNV  + S Y+AKV  F    L I V P  L    + + +SF +TV  
Sbjct: 661 K-PFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSS 720

Query: 547 TV--NQNIVSGSLVWTDGVHQVRSPITVYVV 562
                +  VS +L+W+DG H VRSPI VY +
Sbjct: 721 DSIGTKQPVSANLIWSDGTHNVRSPIIVYAM 740

BLAST of PI0004915 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 2.2e-126
Identity = 266/572 (46.50%), Postives = 358/572 (62.59%), Query Frame = 0

Query: 7   KIIGARTY--RSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVP 66
           K+IGAR Y  + E  P    +S RD+ GHG+HTAS   G+ V   S YGLG GT RGGVP
Sbjct: 180 KLIGARYYTPKLEGFP----ESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVP 239

Query: 67  SARIAVYKICWSD--GCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFH 126
           +ARIAVYK+C      C    ILAAFDDAIAD VDII++S+G   V ++  D++AIGAFH
Sbjct: 240 AARIAVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFH 299

Query: 127 AMKHGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHT 186
           AM  GILT N AGN GPE  T  +++PW  +VAAS ++R F+++V L NG    G ++++
Sbjct: 300 AMAKGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNS 359

Query: 187 FDLMGKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESF 246
           FDL GK+YPL+YG  A   S   ++S + FC    +D   VKGKI++CD+       ++ 
Sbjct: 360 FDLNGKKYPLVYGKSA---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAM 419

Query: 247 NKNGAVGIIMQGSRFKDYASSYPLPASYLHN---------ANITHSSTSTIFKSNAILDA 306
              GAV  I++ + ++D AS +  P S L            N T +  + + KS  I + 
Sbjct: 420 ---GAVASIVR-NPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQ 479

Query: 307 SAPSVVSFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGT 366
            AP V S+SSRGPN    DILKPD+TAPG EILAA+SP  P S    D+R V Y +ISGT
Sbjct: 480 KAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPS--ESDTRHVKYTVISGT 539

Query: 367 SMSCPHATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQ---AEFAYGAGHINPL 426
           SMSCPH   +A Y+KTF+P WSP+ I+SA+MTTA+ MNA  +P    AEFAYGAGH++P+
Sbjct: 540 SMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPI 599

Query: 427 KALNPGLVYNATETDYINFLCGQEGYTTDMVRRITGDKTACTPANSGRVW-DLNYPSFAF 486
            A++PGLVY A ++D+I FLCG   YT   +R I+GD ++CT   +  +  +LNYPS + 
Sbjct: 600 AAIHPGLVYEANKSDHITFLCG-FNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSA 659

Query: 487 STTPSQLIINQFFTRTLTNVEFKASLYSAKVFAPPNLRITVDPPSLLFNGIGDTKSFKLT 546
             + ++      F RT+TNV    + Y AKV     L++ V P  L    + + KSF +T
Sbjct: 660 QVSGTK-PFKVTFRRTVTNVGRPNATYKAKVVG-SKLKVKVVPAVLSLKSLYEKKSFTVT 719

Query: 547 VQGT--VNQNIVSGSLVWTDGVHQVRSPITVY 560
           V G     +N+VS  L+W+DGVH VRSPI VY
Sbjct: 720 VSGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735

BLAST of PI0004915 vs. ExPASy TrEMBL
Match: A0A5A7TIC7 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold158G00080 PE=3 SV=1)

HSP 1 Score: 1060.1 bits (2740), Expect = 3.3e-306
Identity = 534/559 (95.53%), Postives = 543/559 (97.14%), Query Frame = 0

Query: 6   RKIIGARTYRSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVPS 65
           RKIIGARTYRSENLPPGN QSPRDSEGHGTHTASTV G LVSQASLYGLGFGTARGGVPS
Sbjct: 59  RKIIGARTYRSENLPPGNTQSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPS 118

Query: 66  ARIAVYKICWSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHAMK 125
           ARIAVYKICWSDGCYD+DILAAFDDAIADGVDIISLSVGGS VKSYFTDSIAIGAFHAMK
Sbjct: 119 ARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSVVKSYFTDSIAIGAFHAMK 178

Query: 126 HGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHTFDL 185
           HGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANG  +QGPAIHTFDL
Sbjct: 179 HGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGTAYQGPAIHTFDL 238

Query: 186 MGKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESFNKN 245
           MGKQYPLIYGGDAPN+SGGFNSSISRFCDENSVD SLVKGKILVCDSILRASTMESFNKN
Sbjct: 239 MGKQYPLIYGGDAPNKSGGFNSSISRFCDENSVDQSLVKGKILVCDSILRASTMESFNKN 298

Query: 246 GAVGIIMQGSRFKDYASSYPLPASYLHNANITHSSTSTIFKSNAILDASAPSVVSFSSRG 305
           GAVGIIMQGSRFKDYASSYPLPASYLHNANIT SST++IFKSNAILD+SAPSVVSFSSRG
Sbjct: 299 GAVGIIMQGSRFKDYASSYPLPASYLHNANITLSSTASIFKSNAILDSSAPSVVSFSSRG 358

Query: 306 PNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGTSMSCPHATAIAV 365
           PNLATLDILKPDLTAPGVEILAAWSPIA VSGVA DSRSVLYNIISGTSMSCPHATAIAV
Sbjct: 359 PNLATLDILKPDLTAPGVEILAAWSPIATVSGVAGDSRSVLYNIISGTSMSCPHATAIAV 418

Query: 366 YVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKALNPGLVYNATETD 425
           YVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKA+NPGLVYNATETD
Sbjct: 419 YVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKAVNPGLVYNATETD 478

Query: 426 YINFLCGQEGYTTDMVRRITGDKTACTPANSGRVWDLNYPSFAFSTTPSQLIINQFFTRT 485
           YINFLCGQEGYTTDMVRRITGD TACTPANSGRVWDLNYPSFAFSTTPSQL INQFF RT
Sbjct: 479 YINFLCGQEGYTTDMVRRITGDNTACTPANSGRVWDLNYPSFAFSTTPSQLTINQFFART 538

Query: 486 LTNVEFKASLYSAKVFAPPNLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIVSGSLVW 545
           LTNVE K SLYSAKVFAPP+LRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNI+SGSLVW
Sbjct: 539 LTNVESKTSLYSAKVFAPPSLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIISGSLVW 598

Query: 546 TDGVHQVRSPITVYVVKKA 565
           TDGVHQVRSPITVYV KKA
Sbjct: 599 TDGVHQVRSPITVYVAKKA 617

BLAST of PI0004915 vs. ExPASy TrEMBL
Match: A0A1S3CF95 (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500249 PE=3 SV=1)

HSP 1 Score: 1060.1 bits (2740), Expect = 3.3e-306
Identity = 534/559 (95.53%), Postives = 543/559 (97.14%), Query Frame = 0

Query: 6   RKIIGARTYRSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVPS 65
           RKIIGARTYRSENLPPGN QSPRDSEGHGTHTASTV G LVSQASLYGLGFGTARGGVPS
Sbjct: 182 RKIIGARTYRSENLPPGNTQSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPS 241

Query: 66  ARIAVYKICWSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHAMK 125
           ARIAVYKICWSDGCYD+DILAAFDDAIADGVDIISLSVGGS VKSYFTDSIAIGAFHAMK
Sbjct: 242 ARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSVVKSYFTDSIAIGAFHAMK 301

Query: 126 HGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHTFDL 185
           HGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANG  +QGPAIHTFDL
Sbjct: 302 HGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGTAYQGPAIHTFDL 361

Query: 186 MGKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESFNKN 245
           MGKQYPLIYGGDAPN+SGGFNSSISRFCDENSVD SLVKGKILVCDSILRASTMESFNKN
Sbjct: 362 MGKQYPLIYGGDAPNKSGGFNSSISRFCDENSVDQSLVKGKILVCDSILRASTMESFNKN 421

Query: 246 GAVGIIMQGSRFKDYASSYPLPASYLHNANITHSSTSTIFKSNAILDASAPSVVSFSSRG 305
           GAVGIIMQGSRFKDYASSYPLPASYLHNANIT SST++IFKSNAILD+SAPSVVSFSSRG
Sbjct: 422 GAVGIIMQGSRFKDYASSYPLPASYLHNANITLSSTASIFKSNAILDSSAPSVVSFSSRG 481

Query: 306 PNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGTSMSCPHATAIAV 365
           PNLATLDILKPDLTAPGVEILAAWSPIA VSGVA DSRSVLYNIISGTSMSCPHATAIAV
Sbjct: 482 PNLATLDILKPDLTAPGVEILAAWSPIATVSGVAGDSRSVLYNIISGTSMSCPHATAIAV 541

Query: 366 YVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKALNPGLVYNATETD 425
           YVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKA+NPGLVYNATETD
Sbjct: 542 YVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKAVNPGLVYNATETD 601

Query: 426 YINFLCGQEGYTTDMVRRITGDKTACTPANSGRVWDLNYPSFAFSTTPSQLIINQFFTRT 485
           YINFLCGQEGYTTDMVRRITGD TACTPANSGRVWDLNYPSFAFSTTPSQL INQFF RT
Sbjct: 602 YINFLCGQEGYTTDMVRRITGDNTACTPANSGRVWDLNYPSFAFSTTPSQLTINQFFART 661

Query: 486 LTNVEFKASLYSAKVFAPPNLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIVSGSLVW 545
           LTNVE K SLYSAKVFAPP+LRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNI+SGSLVW
Sbjct: 662 LTNVESKTSLYSAKVFAPPSLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIISGSLVW 721

Query: 546 TDGVHQVRSPITVYVVKKA 565
           TDGVHQVRSPITVYV KKA
Sbjct: 722 TDGVHQVRSPITVYVAKKA 740

BLAST of PI0004915 vs. ExPASy TrEMBL
Match: A0A0A0KS99 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G187840 PE=3 SV=1)

HSP 1 Score: 1052.4 bits (2720), Expect = 6.9e-304
Identity = 531/564 (94.15%), Postives = 549/564 (97.34%), Query Frame = 0

Query: 1   MVVSIRKIIGARTYRSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTAR 60
           MVVSIRKIIGARTYRSE LPPGNIQSPRDSEGHGTHTASTV G LVS+ASLYGLGFGTAR
Sbjct: 1   MVVSIRKIIGARTYRSEKLPPGNIQSPRDSEGHGTHTASTVAGGLVSEASLYGLGFGTAR 60

Query: 61  GGVPSARIAVYKICWSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGA 120
           GGVPSARIAVYKICWSDGCYD+DILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGA
Sbjct: 61  GGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGA 120

Query: 121 FHAMKHGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAI 180
           FHA+KHGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANG V+QGPAI
Sbjct: 121 FHAIKHGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGTVYQGPAI 180

Query: 181 HTFDLMGKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTME 240
           HTFDLMGKQYPLI+GGDAPN+SGGFNSSISR+C+ENS+D SLVKGKILVCDSILRAST+E
Sbjct: 181 HTFDLMGKQYPLIHGGDAPNKSGGFNSSISRYCNENSLDLSLVKGKILVCDSILRASTVE 240

Query: 241 SFNKNGAVGIIMQGSRFKDYASSYPLPASYLHNANI-THSSTSTIFKSNAILDASAPSVV 300
           S NKNGAVGIIMQGSRFKDYASSYPLPASYLH+ NI T SST+TIFKSN IL+ASAPSVV
Sbjct: 241 SVNKNGAVGIIMQGSRFKDYASSYPLPASYLHSTNINTLSSTATIFKSNEILNASAPSVV 300

Query: 301 SFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGTSMSCPH 360
           SFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSG+A DSRSVLYNIISGTSMSCPH
Sbjct: 301 SFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGIAGDSRSVLYNIISGTSMSCPH 360

Query: 361 ATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKALNPGLVY 420
           ATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNP+AEFAYGAGHINPLKALNPGLVY
Sbjct: 361 ATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPEAEFAYGAGHINPLKALNPGLVY 420

Query: 421 NATETDYINFLCGQEGYTTDMVRRITGDKTACTPANSGRVWDLNYPSFAFSTTPSQLIIN 480
           NATETDYINFLCGQEGYTT+MVR ITGDKTACTPANSGRVWDLNYPSFAFSTTPSQL IN
Sbjct: 421 NATETDYINFLCGQEGYTTEMVRHITGDKTACTPANSGRVWDLNYPSFAFSTTPSQLTIN 480

Query: 481 QFFTRTLTNVEFKASLYSAKVFAPPNLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIV 540
           QFFTRTLTNVEF  SLY+AKVFAPP+LRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIV
Sbjct: 481 QFFTRTLTNVEFNTSLYTAKVFAPPSLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIV 540

Query: 541 SGSLVWTDGVHQVRSPITVYVVKK 564
           SGSLVWTDGVHQVRSPITVYVVKK
Sbjct: 541 SGSLVWTDGVHQVRSPITVYVVKK 564

BLAST of PI0004915 vs. ExPASy TrEMBL
Match: A0A6J1CBL9 (LOW QUALITY PROTEIN: cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010158 PE=3 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 2.0e-239
Identity = 432/567 (76.19%), Postives = 476/567 (83.95%), Query Frame = 0

Query: 6   RKIIGARTYRSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVPS 65
           +KIIGAR YRSE+LPP +I+SPRDS GHGTHTASTV G LV QASLYGLG GTARGGVPS
Sbjct: 183 KKIIGARVYRSESLPPSDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPS 242

Query: 66  ARIAVYKICWSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHAMK 125
           ARIAVYKICWSDGC D+DILAAFDDAIADGVDIISLSVGGSE  SYF+D IAIGAFHAMK
Sbjct: 243 ARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPNSYFSDPIAIGAFHAMK 302

Query: 126 HGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHTFDL 185
           HGILTSNSAGNEGP YFT SN+SPWSLSVAAST DRK +++VQL NGN++QG AI+TFDL
Sbjct: 303 HGILTSNSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQGVAINTFDL 362

Query: 186 MGKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESFNKN 245
           +GKQYPLIY GDAPN +GGFN SISR C ENSVD +LV+GKILVCDS+L AS + SF  N
Sbjct: 363 LGKQYPLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASF--N 422

Query: 246 GAVGIIMQGSRFKDYASSYPLPASYLHNA---------NITHSSTSTIFKSNAILDASAP 305
             VGI+MQ  R KDYASSYP PASYL  A         + T   T+TI KS A+ DASAP
Sbjct: 423 ATVGIVMQDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAP 482

Query: 306 SVVSFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGTSMS 365
            VVSFSSRGP   T DILK DL+APGVEILAAWSP+APV GV  DSRS LYNIISGTSMS
Sbjct: 483 VVVSFSSRGPKFITRDILKSDLSAPGVEILAAWSPLAPVLGVKGDSRSELYNIISGTSMS 542

Query: 366 CPHATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKALNPG 425
           CPHATAIA YVKTF+PTWSPAA+KSALMTTAF+MNAK+NPQAEFAYG+GH+NPLKA+NPG
Sbjct: 543 CPHATAIAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPG 602

Query: 426 LVYNATETDYINFLCGQEGYTTDMVRRITGDKTACTPANSGRVWDLNYPSFAFSTTPSQL 485
           LVY+A E DY+ FLCGQ GY+T MVRRITGD +ACTP N GRVWDLNYPSFA ST PS+ 
Sbjct: 603 LVYDANEGDYVKFLCGQ-GYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSR- 662

Query: 486 IINQFFTRTLTNVEFKASLYSAKVFAPPN-LRITVDPPSLLFNGIGDTKSFKLTVQGTVN 545
            INQFF RTLTNVE  AS YSA VF PP+ L ITV+P  L FNG+GDTKSF LTVQGTV+
Sbjct: 663 PINQFFRRTLTNVESGASTYSAVVFGPPDGLHITVNPSVLSFNGVGDTKSFNLTVQGTVS 722

Query: 546 QNIVSGSLVWTDGVHQVRSPITVYVVK 563
           Q IVS SL W+DGVHQVRSPIT+Y +K
Sbjct: 723 QAIVSASLEWSDGVHQVRSPITIYFLK 745

BLAST of PI0004915 vs. ExPASy TrEMBL
Match: A0A6J1CEK6 (cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010021 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.4e-232
Identity = 420/569 (73.81%), Postives = 476/569 (83.66%), Query Frame = 0

Query: 6   RKIIGARTYRSEN-LPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVP 65
           +KIIGAR YRS N  PP +I+SPRDS+GHGTHTASTV G LV+QASLYGL  GTARGGVP
Sbjct: 176 KKIIGARAYRSNNFFPPEDIRSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVP 235

Query: 66  SARIAVYKICWSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHAM 125
           SARIAVYKICWSDGCYD+DILAAFDDAIADGVDIISLSVGGSE K YF DSIAIGAFH+M
Sbjct: 236 SARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSM 295

Query: 126 KHGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHTFD 185
           KHGILTSNSAGN+GP+YFT  N SPWSLSVAAS+IDRKFV++VQL N N++QG  I+TFD
Sbjct: 296 KHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKFVTKVQLGNKNLYQGYTINTFD 355

Query: 186 LMGKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESFNK 245
           L+GKQYPLIYGGDAPN +GGF SS SRFC +N+VD +LVKGKILVCD++L  S   SF  
Sbjct: 356 LLGKQYPLIYGGDAPNITGGFTSSSSRFCSKNTVDRNLVKGKILVCDTVLSPSAFASF-- 415

Query: 246 NGAVGIIMQGSRFKDYASSYPLPASYLHNA---------NITHSSTSTIFKSNAILDASA 305
            GAVG++MQ +  KD A SYPLPASYL  A             ++T+TI KSNA+ D SA
Sbjct: 416 XGAVGVVMQDAGVKDNARSYPLPASYLGTAAGGSIKNYMASNRAATATILKSNAVNDTSA 475

Query: 306 PSVVSFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGTSM 365
           P VVSFSSRGPN  T DILKPDLTAPGVEILAAWSPIAP+SG+  DSR  LYNIISGTSM
Sbjct: 476 PLVVSFSSRGPNPETFDILKPDLTAPGVEILAAWSPIAPISGIT-DSRKSLYNIISGTSM 535

Query: 366 SCPHATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKALNP 425
           SCPHATA+AVYVKTF+PTWSPAAIKSALMTTA  +N K+NPQAEFAYGAGH+NPLKA+NP
Sbjct: 536 SCPHATAVAVYVKTFHPTWSPAAIKSALMTTATPLNPKLNPQAEFAYGAGHVNPLKAVNP 595

Query: 426 GLVYNATETDYINFLCGQEGYTTDMVRRITGDKTACTPANSGRVWDLNYPSFAFSTTPSQ 485
           GLVY+A E+DY+ FLCGQ GYTT MV+R+T D +ACTPAN+ RVWDLNYPSFA S+TPS+
Sbjct: 596 GLVYDAHESDYVKFLCGQ-GYTTAMVQRLTADASACTPANTARVWDLNYPSFALSSTPSE 655

Query: 486 LIINQFFTRTLTNVEFKASLYSAKVF-APPNLRITVDPPSLLFNGIGDTKSFKLTVQGTV 545
             INQFFTRTLTNV  KAS Y A V+ APP L I+V PP L F+ IG+ KSFKLT++GT+
Sbjct: 656 -SINQFFTRTLTNVGSKASTYKAAVYGAPPGLTISVSPPVLSFHAIGEKKSFKLTIRGTL 715

Query: 546 NQNIVSGSLVWTDGVHQVRSPITVYVVKK 564
           + +IVS SLVW DG+HQVRSPITVYVV K
Sbjct: 716 SHSIVSASLVWNDGLHQVRSPITVYVVNK 739

BLAST of PI0004915 vs. NCBI nr
Match: KAA0043072.1 (cucumisin-like [Cucumis melo var. makuwa])

HSP 1 Score: 1060.1 bits (2740), Expect = 6.8e-306
Identity = 534/559 (95.53%), Postives = 543/559 (97.14%), Query Frame = 0

Query: 6   RKIIGARTYRSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVPS 65
           RKIIGARTYRSENLPPGN QSPRDSEGHGTHTASTV G LVSQASLYGLGFGTARGGVPS
Sbjct: 59  RKIIGARTYRSENLPPGNTQSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPS 118

Query: 66  ARIAVYKICWSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHAMK 125
           ARIAVYKICWSDGCYD+DILAAFDDAIADGVDIISLSVGGS VKSYFTDSIAIGAFHAMK
Sbjct: 119 ARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSVVKSYFTDSIAIGAFHAMK 178

Query: 126 HGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHTFDL 185
           HGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANG  +QGPAIHTFDL
Sbjct: 179 HGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGTAYQGPAIHTFDL 238

Query: 186 MGKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESFNKN 245
           MGKQYPLIYGGDAPN+SGGFNSSISRFCDENSVD SLVKGKILVCDSILRASTMESFNKN
Sbjct: 239 MGKQYPLIYGGDAPNKSGGFNSSISRFCDENSVDQSLVKGKILVCDSILRASTMESFNKN 298

Query: 246 GAVGIIMQGSRFKDYASSYPLPASYLHNANITHSSTSTIFKSNAILDASAPSVVSFSSRG 305
           GAVGIIMQGSRFKDYASSYPLPASYLHNANIT SST++IFKSNAILD+SAPSVVSFSSRG
Sbjct: 299 GAVGIIMQGSRFKDYASSYPLPASYLHNANITLSSTASIFKSNAILDSSAPSVVSFSSRG 358

Query: 306 PNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGTSMSCPHATAIAV 365
           PNLATLDILKPDLTAPGVEILAAWSPIA VSGVA DSRSVLYNIISGTSMSCPHATAIAV
Sbjct: 359 PNLATLDILKPDLTAPGVEILAAWSPIATVSGVAGDSRSVLYNIISGTSMSCPHATAIAV 418

Query: 366 YVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKALNPGLVYNATETD 425
           YVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKA+NPGLVYNATETD
Sbjct: 419 YVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKAVNPGLVYNATETD 478

Query: 426 YINFLCGQEGYTTDMVRRITGDKTACTPANSGRVWDLNYPSFAFSTTPSQLIINQFFTRT 485
           YINFLCGQEGYTTDMVRRITGD TACTPANSGRVWDLNYPSFAFSTTPSQL INQFF RT
Sbjct: 479 YINFLCGQEGYTTDMVRRITGDNTACTPANSGRVWDLNYPSFAFSTTPSQLTINQFFART 538

Query: 486 LTNVEFKASLYSAKVFAPPNLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIVSGSLVW 545
           LTNVE K SLYSAKVFAPP+LRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNI+SGSLVW
Sbjct: 539 LTNVESKTSLYSAKVFAPPSLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIISGSLVW 598

Query: 546 TDGVHQVRSPITVYVVKKA 565
           TDGVHQVRSPITVYV KKA
Sbjct: 599 TDGVHQVRSPITVYVAKKA 617

BLAST of PI0004915 vs. NCBI nr
Match: XP_008461717.1 (PREDICTED: cucumisin-like [Cucumis melo])

HSP 1 Score: 1060.1 bits (2740), Expect = 6.8e-306
Identity = 534/559 (95.53%), Postives = 543/559 (97.14%), Query Frame = 0

Query: 6   RKIIGARTYRSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVPS 65
           RKIIGARTYRSENLPPGN QSPRDSEGHGTHTASTV G LVSQASLYGLGFGTARGGVPS
Sbjct: 182 RKIIGARTYRSENLPPGNTQSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPS 241

Query: 66  ARIAVYKICWSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHAMK 125
           ARIAVYKICWSDGCYD+DILAAFDDAIADGVDIISLSVGGS VKSYFTDSIAIGAFHAMK
Sbjct: 242 ARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSVVKSYFTDSIAIGAFHAMK 301

Query: 126 HGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHTFDL 185
           HGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANG  +QGPAIHTFDL
Sbjct: 302 HGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGTAYQGPAIHTFDL 361

Query: 186 MGKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESFNKN 245
           MGKQYPLIYGGDAPN+SGGFNSSISRFCDENSVD SLVKGKILVCDSILRASTMESFNKN
Sbjct: 362 MGKQYPLIYGGDAPNKSGGFNSSISRFCDENSVDQSLVKGKILVCDSILRASTMESFNKN 421

Query: 246 GAVGIIMQGSRFKDYASSYPLPASYLHNANITHSSTSTIFKSNAILDASAPSVVSFSSRG 305
           GAVGIIMQGSRFKDYASSYPLPASYLHNANIT SST++IFKSNAILD+SAPSVVSFSSRG
Sbjct: 422 GAVGIIMQGSRFKDYASSYPLPASYLHNANITLSSTASIFKSNAILDSSAPSVVSFSSRG 481

Query: 306 PNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGTSMSCPHATAIAV 365
           PNLATLDILKPDLTAPGVEILAAWSPIA VSGVA DSRSVLYNIISGTSMSCPHATAIAV
Sbjct: 482 PNLATLDILKPDLTAPGVEILAAWSPIATVSGVAGDSRSVLYNIISGTSMSCPHATAIAV 541

Query: 366 YVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKALNPGLVYNATETD 425
           YVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKA+NPGLVYNATETD
Sbjct: 542 YVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKAVNPGLVYNATETD 601

Query: 426 YINFLCGQEGYTTDMVRRITGDKTACTPANSGRVWDLNYPSFAFSTTPSQLIINQFFTRT 485
           YINFLCGQEGYTTDMVRRITGD TACTPANSGRVWDLNYPSFAFSTTPSQL INQFF RT
Sbjct: 602 YINFLCGQEGYTTDMVRRITGDNTACTPANSGRVWDLNYPSFAFSTTPSQLTINQFFART 661

Query: 486 LTNVEFKASLYSAKVFAPPNLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIVSGSLVW 545
           LTNVE K SLYSAKVFAPP+LRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNI+SGSLVW
Sbjct: 662 LTNVESKTSLYSAKVFAPPSLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIISGSLVW 721

Query: 546 TDGVHQVRSPITVYVVKKA 565
           TDGVHQVRSPITVYV KKA
Sbjct: 722 TDGVHQVRSPITVYVAKKA 740

BLAST of PI0004915 vs. NCBI nr
Match: KGN50586.1 (hypothetical protein Csa_021462 [Cucumis sativus])

HSP 1 Score: 1052.4 bits (2720), Expect = 1.4e-303
Identity = 531/564 (94.15%), Postives = 549/564 (97.34%), Query Frame = 0

Query: 1   MVVSIRKIIGARTYRSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTAR 60
           MVVSIRKIIGARTYRSE LPPGNIQSPRDSEGHGTHTASTV G LVS+ASLYGLGFGTAR
Sbjct: 1   MVVSIRKIIGARTYRSEKLPPGNIQSPRDSEGHGTHTASTVAGGLVSEASLYGLGFGTAR 60

Query: 61  GGVPSARIAVYKICWSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGA 120
           GGVPSARIAVYKICWSDGCYD+DILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGA
Sbjct: 61  GGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGA 120

Query: 121 FHAMKHGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAI 180
           FHA+KHGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANG V+QGPAI
Sbjct: 121 FHAIKHGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGTVYQGPAI 180

Query: 181 HTFDLMGKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTME 240
           HTFDLMGKQYPLI+GGDAPN+SGGFNSSISR+C+ENS+D SLVKGKILVCDSILRAST+E
Sbjct: 181 HTFDLMGKQYPLIHGGDAPNKSGGFNSSISRYCNENSLDLSLVKGKILVCDSILRASTVE 240

Query: 241 SFNKNGAVGIIMQGSRFKDYASSYPLPASYLHNANI-THSSTSTIFKSNAILDASAPSVV 300
           S NKNGAVGIIMQGSRFKDYASSYPLPASYLH+ NI T SST+TIFKSN IL+ASAPSVV
Sbjct: 241 SVNKNGAVGIIMQGSRFKDYASSYPLPASYLHSTNINTLSSTATIFKSNEILNASAPSVV 300

Query: 301 SFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGTSMSCPH 360
           SFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSG+A DSRSVLYNIISGTSMSCPH
Sbjct: 301 SFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGIAGDSRSVLYNIISGTSMSCPH 360

Query: 361 ATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKALNPGLVY 420
           ATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNP+AEFAYGAGHINPLKALNPGLVY
Sbjct: 361 ATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPEAEFAYGAGHINPLKALNPGLVY 420

Query: 421 NATETDYINFLCGQEGYTTDMVRRITGDKTACTPANSGRVWDLNYPSFAFSTTPSQLIIN 480
           NATETDYINFLCGQEGYTT+MVR ITGDKTACTPANSGRVWDLNYPSFAFSTTPSQL IN
Sbjct: 421 NATETDYINFLCGQEGYTTEMVRHITGDKTACTPANSGRVWDLNYPSFAFSTTPSQLTIN 480

Query: 481 QFFTRTLTNVEFKASLYSAKVFAPPNLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIV 540
           QFFTRTLTNVEF  SLY+AKVFAPP+LRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIV
Sbjct: 481 QFFTRTLTNVEFNTSLYTAKVFAPPSLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIV 540

Query: 541 SGSLVWTDGVHQVRSPITVYVVKK 564
           SGSLVWTDGVHQVRSPITVYVVKK
Sbjct: 541 SGSLVWTDGVHQVRSPITVYVVKK 564

BLAST of PI0004915 vs. NCBI nr
Match: XP_031741167.1 (cucumisin [Cucumis sativus])

HSP 1 Score: 1044.3 bits (2699), Expect = 3.9e-301
Identity = 526/559 (94.10%), Postives = 544/559 (97.32%), Query Frame = 0

Query: 6   RKIIGARTYRSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVPS 65
           RKIIGARTYRSE LPPGNIQSPRDSEGHGTHTASTV G LVS+ASLYGLGFGTARGGVPS
Sbjct: 163 RKIIGARTYRSEKLPPGNIQSPRDSEGHGTHTASTVAGGLVSEASLYGLGFGTARGGVPS 222

Query: 66  ARIAVYKICWSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHAMK 125
           ARIAVYKICWSDGCYD+DILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHA+K
Sbjct: 223 ARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHAIK 282

Query: 126 HGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHTFDL 185
           HGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANG V+QGPAIHTFDL
Sbjct: 283 HGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGTVYQGPAIHTFDL 342

Query: 186 MGKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESFNKN 245
           MGKQYPLI+GGDAPN+SGGFNSSISR+C+ENS+D SLVKGKILVCDSILRAST+ES NKN
Sbjct: 343 MGKQYPLIHGGDAPNKSGGFNSSISRYCNENSLDLSLVKGKILVCDSILRASTVESVNKN 402

Query: 246 GAVGIIMQGSRFKDYASSYPLPASYLHNANI-THSSTSTIFKSNAILDASAPSVVSFSSR 305
           GAVGIIMQGSRFKDYASSYPLPASYLH+ NI T SST+TIFKSN IL+ASAPSVVSFSSR
Sbjct: 403 GAVGIIMQGSRFKDYASSYPLPASYLHSTNINTLSSTATIFKSNEILNASAPSVVSFSSR 462

Query: 306 GPNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGTSMSCPHATAIA 365
           GPNLATLDILKPDLTAPGVEILAAWSPIAPVSG+A DSRSVLYNIISGTSMSCPHATAIA
Sbjct: 463 GPNLATLDILKPDLTAPGVEILAAWSPIAPVSGIAGDSRSVLYNIISGTSMSCPHATAIA 522

Query: 366 VYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKALNPGLVYNATET 425
           VYVKTFNPTWSPAAIKSALMTTAFSMNAKVNP+AEFAYGAGHINPLKALNPGLVYNATET
Sbjct: 523 VYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPEAEFAYGAGHINPLKALNPGLVYNATET 582

Query: 426 DYINFLCGQEGYTTDMVRRITGDKTACTPANSGRVWDLNYPSFAFSTTPSQLIINQFFTR 485
           DYINFLCGQEGYTT+MVR ITGDKTACTPANSGRVWDLNYPSFAFSTTPSQL INQFFTR
Sbjct: 583 DYINFLCGQEGYTTEMVRHITGDKTACTPANSGRVWDLNYPSFAFSTTPSQLTINQFFTR 642

Query: 486 TLTNVEFKASLYSAKVFAPPNLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIVSGSLV 545
           TLTNVEF  SLY+AKVFAPP+LRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIVSGSLV
Sbjct: 643 TLTNVEFNTSLYTAKVFAPPSLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIVSGSLV 702

Query: 546 WTDGVHQVRSPITVYVVKK 564
           WTDGVHQVRSPITVYVVKK
Sbjct: 703 WTDGVHQVRSPITVYVVKK 721

BLAST of PI0004915 vs. NCBI nr
Match: XP_038891007.1 (cucumisin-like [Benincasa hispida])

HSP 1 Score: 991.1 bits (2561), Expect = 3.9e-285
Identity = 499/559 (89.27%), Postives = 521/559 (93.20%), Query Frame = 0

Query: 6   RKIIGARTYRSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVPS 65
           RKIIGAR YRSEN  P +IQSPRDSEGHGTHTASTV G LVSQASLYGLGFGTARGGVPS
Sbjct: 179 RKIIGARLYRSENFSPEDIQSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPS 238

Query: 66  ARIAVYKICWSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHAMK 125
           ARIAVYKICWSDGCYD+DILAAFDDAIADGVDIISLSVGGSE KSYF DSIAIGAFHAM+
Sbjct: 239 ARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKSYFNDSIAIGAFHAMQ 298

Query: 126 HGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHTFDL 185
           HGILTSNSAGNEGPEYFTTSN+SPWSLSVAAST+DRKFVS+VQLANG ++QG AIHTFDL
Sbjct: 299 HGILTSNSAGNEGPEYFTTSNISPWSLSVAASTMDRKFVSQVQLANGTIYQGVAIHTFDL 358

Query: 186 MGKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESFNKN 245
           MGKQ+PLIYGGDAPN++GGFNSS SRFCDE SVDPSLVKGKILVCDS+LRASTMESFN N
Sbjct: 359 MGKQHPLIYGGDAPNKAGGFNSSTSRFCDEKSVDPSLVKGKILVCDSLLRASTMESFNNN 418

Query: 246 GAVGIIMQGSRFKDYASSYPLPASYLHNANITHSSTSTIFKSNAILDASAPSVVSFSSRG 305
           G VGIIMQGSR KDYASSYPLPASYLHN +I  SST+TIFKSNA LDASAPSVVSFSSRG
Sbjct: 419 GVVGIIMQGSRLKDYASSYPLPASYLHNEDIKLSSTATIFKSNANLDASAPSVVSFSSRG 478

Query: 306 PNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGTSMSCPHATAIAV 365
           PNL TLDILKPDLTAPGVEILAAWSPIAPVSG A D+RSVLYNIISGTSMSCPHATAIAV
Sbjct: 479 PNLVTLDILKPDLTAPGVEILAAWSPIAPVSGTARDTRSVLYNIISGTSMSCPHATAIAV 538

Query: 366 YVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKALNPGLVYNATETD 425
           YVKTF+PTWSPAAIKSALMTTAFSMN KVNPQAEFAYGAGHINPLKA+NPGLVYNA+ETD
Sbjct: 539 YVKTFHPTWSPAAIKSALMTTAFSMNGKVNPQAEFAYGAGHINPLKAINPGLVYNASETD 598

Query: 426 YINFLCGQEGYTTDMVRRITGDKTACTPANSGRVWDLNYPSFAFSTTPSQLIINQFFTRT 485
           YI FLCG EGYTTDMVR ITGD TACTP NSGRVWDLNYPSFA STTPSQ  INQFFTRT
Sbjct: 599 YIKFLCGHEGYTTDMVRHITGDNTACTPTNSGRVWDLNYPSFALSTTPSQ-SINQFFTRT 658

Query: 486 LTNVEFKASLYSAKVFAPPNLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIVSGSLVW 545
           LTNVEF+ SLYSA VFAPP+LRITV+PP L FNGIGDTKSFKLTVQGTV+Q IVS SLVW
Sbjct: 659 LTNVEFRTSLYSAMVFAPPSLRITVEPPLLSFNGIGDTKSFKLTVQGTVSQAIVSASLVW 718

Query: 546 TDGVHQVRSPITVYVVKKA 565
           TDGVHQVRSPITVYVV KA
Sbjct: 719 TDGVHQVRSPITVYVVNKA 736

BLAST of PI0004915 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 466.5 bits (1199), Expect = 3.1e-131
Identity = 269/576 (46.70%), Postives = 357/576 (61.98%), Query Frame = 0

Query: 7   KIIGARTYRSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVPSA 66
           K+IGAR Y           S RD EGHGTHTAST  G+ V  AS YGL  GTARGGVPSA
Sbjct: 140 KLIGARFYNK------FADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSA 199

Query: 67  RIAVYKICWSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHAMKH 126
           RIA YK+C+ + C D DILAAFDDAIADGVD+IS+S+    V +    S+AIG+FHAM  
Sbjct: 200 RIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMR 259

Query: 127 GILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHTFDLM 186
           GI+T+ SAGN GP+  + +NVSPW ++VAAS  DR+F+ RV L NG    G +++TF+L 
Sbjct: 260 GIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLN 319

Query: 187 GKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESFNKNG 246
           G ++P++YG    N S   + + + +C    VD  LVKGKI++CD  L     E++   G
Sbjct: 320 GTKFPIVYG---QNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFL--GYREAY-LAG 379

Query: 247 AVGIIMQGSRFKDYASSYPLPA------------SYLHNANITHSSTSTIFKSNAILDAS 306
           A+G+I+Q +   D A   P PA            SY+ +A       + I ++  I+D  
Sbjct: 380 AIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESA---EPPQAEILRTEEIVDRE 439

Query: 307 APSVVSFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGV--AEDSRSVLYNIISG 366
           AP V SFSSRGP+    ++LKPD++APG+EILAA+SP+A  S     ED RSV Y+++SG
Sbjct: 440 APYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSG 499

Query: 367 TSMSCPHATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKA 426
           TSM+CPH   +A YVK+F+P WSP+AIKSA+MTTA  MN K NP+ EFAYG+G INP KA
Sbjct: 500 TSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKA 559

Query: 427 LNPGLVYNATETDYINFLCGQEGYTTDMVRRITGDKTACTPANSGRVWDLNYP---SFAF 486
            +PGLVY     DY+  LC  EG+ +  +   +G    C+      V DLNYP   +F  
Sbjct: 560 SDPGLVYEVETEDYLKMLCA-EGFDSTTLTTTSGQNVTCSERT--EVKDLNYPTMTTFVS 619

Query: 487 STTPSQLIINQFFTRTLTNVEFKASLYSAKVF-APPNLRITVDPPSLLFNGIGDTKSFKL 546
           S  P     N  F RT+TNV F  S Y A V    P L+I+++P  L F  + + KSF +
Sbjct: 620 SLDP----FNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVV 679

Query: 547 TVQG--TVNQNIVSGSLVWTDGVHQVRSPITVYVVK 563
           T+ G    + + VS S+VW+DG H VRSPI  Y ++
Sbjct: 680 TISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSIQ 692

BLAST of PI0004915 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 462.6 bits (1189), Expect = 4.5e-130
Identity = 263/571 (46.06%), Postives = 356/571 (62.35%), Query Frame = 0

Query: 7   KIIGARTYRSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVPSA 66
           K+IGAR Y SE          RD +GHGTHTAST  G+ V  AS +G+G GT RGGVP++
Sbjct: 178 KLIGARDYTSE--------GTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPAS 237

Query: 67  RIAVYKICWSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHAMKH 126
           R+A YK+C   GC    +L+AFDDAIADGVD+I++S+G      +  D IAIGAFHAM  
Sbjct: 238 RVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAK 297

Query: 127 GILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHTFDLM 186
           G+LT NSAGN GP+  + S V+PW L+VAAST +R FV++V L NG    G +++ +++ 
Sbjct: 298 GVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMK 357

Query: 187 GKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESFNKNG 246
           GK YPL+YG  A   S   ++  +  C+ + VD S VKGKILVC        +ES    G
Sbjct: 358 GKDYPLVYGKSA--ASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV---G 417

Query: 247 AVGIIMQGSRFKDYASSYPLPA------------SYLHNANITHSSTSTIFKSNAILDAS 306
           AVG+I +  +  D A  +PLPA            SYL +   T S  + + K+ AI + +
Sbjct: 418 AVGLIYRTPK-PDVAFIHPLPAAGLLTEDFESLVSYLES---TDSPQAIVLKTEAIFNRT 477

Query: 307 APSVVSFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGTS 366
           +P + SFSSRGPN   +DILKPD+TAPGVEILAA+SP    S   +D+R V Y+++SGTS
Sbjct: 478 SPVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTS 537

Query: 367 MSCPHATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVN--PQAEFAYGAGHINPLKA 426
           MSCPH   +A YVKTFNP WSP+ I+SA+MTTA+ +NA        EFAYG+GH++P+ A
Sbjct: 538 MSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAA 597

Query: 427 LNPGLVYNATETDYINFLCGQEGYTTDMVRRITGDKTACTPANSGRVWDLNYPSFAFSTT 486
            NPGLVY   ++D+I FLCG   YT+ +++ I+G+   C+ A      +LNYPS +   +
Sbjct: 598 SNPGLVYELDKSDHIAFLCGM-NYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLS 657

Query: 487 PSQLIINQFFTRTLTNVEFKASLYSAKVFA--PPNLRITVDPPSLLFNGIGDTKSFKLTV 546
            S       F RTLTNV    S Y++KV A     L + + P  L F  + + +SF +TV
Sbjct: 658 GSGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTV 717

Query: 547 QGTVNQNIV--SGSLVWTDGVHQVRSPITVY 560
            G+   + V  S +L+W+DG H VRSPI VY
Sbjct: 718 TGSNLDSEVPSSANLIWSDGTHNVRSPIVVY 728

BLAST of PI0004915 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 455.3 bits (1170), Expect = 7.2e-128
Identity = 260/571 (45.53%), Postives = 361/571 (63.22%), Query Frame = 0

Query: 7   KIIGARTYRSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVPSA 66
           K+IGAR Y +++      Q+ RD  GHGTHTAS   G+ V+ ++ YGLG GTARGGVP+A
Sbjct: 181 KVIGARDYTAKSKAN---QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAA 240

Query: 67  RIAVYKICWSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHAMKH 126
           RIAVYK+C ++GC    +++AFDDAIADGVD+IS+S+    +  +  D IAIGAFHAM  
Sbjct: 241 RIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAV 300

Query: 127 GILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHTFDLM 186
           G+LT N+AGN GP+  T ++ +PW  SVAAS  +R F+++V L +G +  G +++T+D+ 
Sbjct: 301 GVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMN 360

Query: 187 GKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESFNKNG 246
           G  YPL+YG  A   +   +   +R C+   +D  LVKGKI++CDS      +    K G
Sbjct: 361 GTNYPLVYGKSAALSTCSVDK--ARLCEPKCLDGKLVKGKIVLCDS---TKGLIEAQKLG 420

Query: 247 AVGIIMQGSRFKDYASSYPLPASYLHN---------ANITHSSTSTIFKSNAILDASAPS 306
           AVG I++     D A     P S+L N          N T +  +T+ KS  I +  AP 
Sbjct: 421 AVGSIVKNPE-PDRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPL 480

Query: 307 VVSFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGTSMSC 366
           V SFSSRGP+    DILKPD+TAPGVEILAA+SP +  +    D+R V Y+++SGTSM+C
Sbjct: 481 VASFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMAC 540

Query: 367 PHATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVN--PQAEFAYGAGHINPLKALNP 426
           PH   +A YVKTF+P WSP+ I+SA+MTTA+ MNA  +     EFAYG+GH++P+ A+NP
Sbjct: 541 PHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINP 600

Query: 427 GLVYNATETDYINFLCGQEGYTTDMVRRITGDKTACTPANSGRV-WDLNYPSFAFSTTPS 486
           GLVY  T+ D+INFLCG   YT+D +R I+GD + CT   S  +  +LNYP+ +   + +
Sbjct: 601 GLVYELTKADHINFLCGL-NYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGT 660

Query: 487 QLIINQFFTRTLTNVEFKASLYSAKV--FAPPNLRITVDPPSLLFNGIGDTKSFKLTVQG 546
           +   N  F RT+TNV  + S Y+AKV  F    L I V P  L    + + +SF +TV  
Sbjct: 661 K-PFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSS 720

Query: 547 TV--NQNIVSGSLVWTDGVHQVRSPITVYVV 562
                +  VS +L+W+DG H VRSPI VY +
Sbjct: 721 DSIGTKQPVSANLIWSDGTHNVRSPIIVYAM 740

BLAST of PI0004915 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 454.1 bits (1167), Expect = 1.6e-127
Identity = 266/572 (46.50%), Postives = 358/572 (62.59%), Query Frame = 0

Query: 7   KIIGARTY--RSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVP 66
           K+IGAR Y  + E  P    +S RD+ GHG+HTAS   G+ V   S YGLG GT RGGVP
Sbjct: 180 KLIGARYYTPKLEGFP----ESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVP 239

Query: 67  SARIAVYKICWSD--GCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFH 126
           +ARIAVYK+C      C    ILAAFDDAIAD VDII++S+G   V ++  D++AIGAFH
Sbjct: 240 AARIAVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFH 299

Query: 127 AMKHGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHT 186
           AM  GILT N AGN GPE  T  +++PW  +VAAS ++R F+++V L NG    G ++++
Sbjct: 300 AMAKGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNS 359

Query: 187 FDLMGKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESF 246
           FDL GK+YPL+YG  A   S   ++S + FC    +D   VKGKI++CD+       ++ 
Sbjct: 360 FDLNGKKYPLVYGKSA---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAM 419

Query: 247 NKNGAVGIIMQGSRFKDYASSYPLPASYLHN---------ANITHSSTSTIFKSNAILDA 306
              GAV  I++ + ++D AS +  P S L            N T +  + + KS  I + 
Sbjct: 420 ---GAVASIVR-NPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQ 479

Query: 307 SAPSVVSFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGT 366
            AP V S+SSRGPN    DILKPD+TAPG EILAA+SP  P S    D+R V Y +ISGT
Sbjct: 480 KAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPS--ESDTRHVKYTVISGT 539

Query: 367 SMSCPHATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNPQ---AEFAYGAGHINPL 426
           SMSCPH   +A Y+KTF+P WSP+ I+SA+MTTA+ MNA  +P    AEFAYGAGH++P+
Sbjct: 540 SMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPI 599

Query: 427 KALNPGLVYNATETDYINFLCGQEGYTTDMVRRITGDKTACTPANSGRVW-DLNYPSFAF 486
            A++PGLVY A ++D+I FLCG   YT   +R I+GD ++CT   +  +  +LNYPS + 
Sbjct: 600 AAIHPGLVYEANKSDHITFLCG-FNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSA 659

Query: 487 STTPSQLIINQFFTRTLTNVEFKASLYSAKVFAPPNLRITVDPPSLLFNGIGDTKSFKLT 546
             + ++      F RT+TNV    + Y AKV     L++ V P  L    + + KSF +T
Sbjct: 660 QVSGTK-PFKVTFRRTVTNVGRPNATYKAKVVG-SKLKVKVVPAVLSLKSLYEKKSFTVT 719

Query: 547 VQGT--VNQNIVSGSLVWTDGVHQVRSPITVY 560
           V G     +N+VS  L+W+DGVH VRSPI VY
Sbjct: 720 VSGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735

BLAST of PI0004915 vs. TAIR 10
Match: AT3G46840.1 (Subtilase family protein )

HSP 1 Score: 453.0 bits (1164), Expect = 3.6e-127
Identity = 268/572 (46.85%), Postives = 356/572 (62.24%), Query Frame = 0

Query: 7   KIIGARTY--RSENLPPGNIQSPRDSEGHGTHTASTVVGSLVSQASLYGLGFGTARGGVP 66
           K+IGAR Y  + E  P    +S RD  GHG+HTAST  G+ V   S YGLG GTARGGVP
Sbjct: 181 KLIGARYYTPKLEGFP----ESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVP 240

Query: 67  SARIAVYKIC--WSDGCYDSDILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFH 126
           +ARIAVYK+C    DGC    ILAAFDDAIAD VDII++S+GG     +  D IAIGAFH
Sbjct: 241 AARIAVYKVCDPGVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFH 300

Query: 127 AMKHGILTSNSAGNEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGNVHQGPAIHT 186
           AM  GIL  NSAGN GPE  T ++++PW  +VAAS  +R FV++V L NG    G ++++
Sbjct: 301 AMAKGILIVNSAGNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKT-VGRSVNS 360

Query: 187 FDLMGKQYPLIYGGDAPNQSGGFNSSISRFCDENSVDPSLVKGKILVCDSILRASTMESF 246
           FDL GK+YPL+YG  A +  G  ++    FC    +D   VKGKI++CDS       ++ 
Sbjct: 361 FDLNGKKYPLVYGKSASSSCGAASAG---FCSPGCLDSKRVKGKIVLCDSPQNPDEAQAM 420

Query: 247 NKNGAVGIIMQGSRFKDYASSYPLPASYL----HNA-----NITHSSTSTIFKSNAILDA 306
              GA+  I++  R  D AS +  P S L    +N      N T +  + + KS  I + 
Sbjct: 421 ---GAIASIVRSHR-TDVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQ 480

Query: 307 SAPSVVSFSSRGPNLATLDILKPDLTAPGVEILAAWSPIAPVSGVAEDSRSVLYNIISGT 366
            AP V S+ SRGPN    DILKPD+TAPG EI+AA+SP AP S    D+R V Y++ +GT
Sbjct: 481 RAPVVASYFSRGPNTIIPDILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGT 540

Query: 367 SMSCPHATAIAVYVKTFNPTWSPAAIKSALMTTAFSMNAKVNP---QAEFAYGAGHINPL 426
           SMSCPH   +A Y+K+F+P WSP+ I+SA+MTTA+ MNA  +P    AEFAYGAGH++P+
Sbjct: 541 SMSCPHVAGVAAYLKSFHPRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPI 600

Query: 427 KALNPGLVYNATETDYINFLCGQEGYTTDMVRRITGDKTACTPANSGRV-WDLNYPSFAF 486
            A++PGLVY A ++D+I FLCG   YT   +R I+GD ++CT   +  +  +LNYPS   
Sbjct: 601 TAIHPGLVYEANKSDHIAFLCGL-NYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTA 660

Query: 487 STTPSQLIINQFFTRTLTNVEFKASLYSAKVFAPPNLRITVDPPSLLFNGIGDTKSFKLT 546
             + ++      F RT+TNV    + Y AKV     L++ V P  L    + + KSF +T
Sbjct: 661 QVSAAK-PFKVIFRRTVTNVGRPNATYKAKVVG-SKLKVKVVPAVLSLKSLYEKKSFTVT 720

Query: 547 VQGT--VNQNIVSGSLVWTDGVHQVRSPITVY 560
             G     +N+VS  L+W+DGVH VRSPI VY
Sbjct: 721 ASGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735