PI0004912 (gene) Melon (PI 482460) v1

BLAST of PI0004912 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 988.8 bits (2555), Expect = 3.5e-287
Identity = 494/764 (64.66%), Postives = 604/764 (79.06%), Query Frame = 0

Query: 9   WFLLFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSSLKSVSDSA 68
           +FLL  + F          S+    + TYI+HM K+ MP +FD H  WYDSSL+S+SDSA
Sbjct: 11  FFLLLCLGF-------CHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA 70

Query: 69  QMLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLGLGK-SVSF 128
           ++LY+Y   IHGFSTRLT EEA  +  Q G+++V PE +YELHTTRTP FLGL + +   
Sbjct: 71  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 130

Query: 129 FPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSCNRKLIGA 188
           FP +   S V++GVLDTGVWPE +S+SD+G GPIP+SWKG CE G NFT+S CNRKLIGA
Sbjct: 131 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 190

Query: 189 RYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAGTARGMAA 248
           R+F++GYE+  GPIDES+ES+SPRDDDGHG+HTS+TAAGS V GA+L G+A+GTARGMA 
Sbjct: 191 RFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAP 250

Query: 249 EARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNTADFYRDNVAIGAFSAAA 308
            ARVA YKVCWLGGCFSSDILAA+DK++ D  N+LS+SLGG  +D+YRD VAIGAF+A  
Sbjct: 251 RARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAME 310

Query: 309 QGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGESLYSGKP 368
           +G+ VSCSAGN GPSSS+LSNVAPWITTVGAGTLDRDFPA   LGNGK  TG SL+ G+ 
Sbjct: 311 RGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEA 370

Query: 369 LPNSLLPIVYAASASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQKGLVVKEAGGL 428
           LP+ LLP +YA +ASN+++G+LC++ TL P KV GKIV+CDRG N+RVQKG VVK AGG+
Sbjct: 371 LPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGV 430

Query: 429 GMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYISSDSNPTATISTGTTRLGVQPS 488
           GMILANT A GEE +ADAHL+P   VG+K+GD I+ Y+++D NPTA+IS   T +GV+PS
Sbjct: 431 GMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPS 490

Query: 489 PVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFNIISGTSM 548
           PVVAAFSSRGPN ITP ILKPDLIAPGVNILA WTG AGPTGL SD R V FNIISGTSM
Sbjct: 491 PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSM 550

Query: 549 SCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTPFDIGAGH 608
           SCPH+SGLAALLK+ HP+WSPAAIRSALMTTAY TYK+G+ + DI+ G PSTPFD GAGH
Sbjct: 551 SCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGH 610

Query: 609 VNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLEDLNYPSF 668
           V+PT A +PGL+YD TT+DYL FLCALNY+S QI+ +S++++TC+ +K+Y + DLNYPSF
Sbjct: 611 VSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSF 670

Query: 669 AVPLETPSTKGGENVAPTTVKYTRTLTNKGAPSTYKVSVTAKSSSVKILVEPESLSFTEA 728
           AV ++               KYTRT+T+ G   TY V VT++++ VKI VEP  L+F EA
Sbjct: 671 AVNVD----------GVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEA 730

Query: 729 NEQKSYTVTFTA-SPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 771
           NE+KSYTVTFT  S  PSGS SF  +EWSDGKH+VGSP+A +WT
Sbjct: 731 NEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of PI0004912 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 765.8 bits (1976), Expect = 4.8e-220
Identity = 414/741 (55.87%), Postives = 515/741 (69.50%), Query Frame = 0

Query: 34  KKTYIIHMDKANMPQAFDDHFQWYDSSLKSVSDSAQMLYSYNTVIHGFSTRLTVEEA-KL 93
           KKTYII ++ ++ P++F  H  WY S L S S    +LY+Y T  HGFS  L   EA  L
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTTSFHGFSAYLDSTEADSL 86

Query: 94  MEKQEGILAVTPEMKYELHTTRTPEFLGLGKSVSFFPASVKVSGVIIGVLDTGVWPELES 153
           +     IL +  +  Y LHTTRTPEFLGL             +GVIIGVLDTGVWPE  S
Sbjct: 87  LSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRS 146

Query: 154 FSDDGLGPIPASWKGECEVGKNFTSSSCNRKLIGARYFSKGYE-AAFGPIDESQESKSPR 213
           F D  +  IP+ WKGECE G +F S  CN+KLIGAR FSKG++ A+ G     +ES SPR
Sbjct: 147 FDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPR 206

Query: 214 DDDGHGSHTSTTAAGSAVTGANLFGFAAGTARGMAAEARVATYKVCWLGGCFSSDILAAM 273
           D DGHG+HTSTTAAGSAV  A+  G+AAGTARGMA  ARVATYKVCW  GCF SDILAAM
Sbjct: 207 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAM 266

Query: 274 DKSVEDGCNILSVSLGGNTADFYRDNVAIGAFSAAAQGVFVSCSAGNGGPSSSTLSNVAP 333
           D+++ DG ++LS+SLGG +A +YRD +AIGAFSA  +GVFVSCSAGN GP+ ++++NVAP
Sbjct: 267 DRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAP 326

Query: 334 WITTVGAGTLDRDFPAYVTLGNGKKITGESLYSGKPLPNSLLPIVYAASASNSSSGSLCL 393
           W+ TVGAGTLDRDFPA+  LGNGK++TG SLYSG  +    L +VY  +  NSSS +LCL
Sbjct: 327 WVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY--NKGNSSSSNLCL 386

Query: 394 SSTLNPAKVVGKIVVCDRGGNSRVQKGLVVKEAGGLGMILANTEAYGEEQLADAHLIPTA 453
             +L+ + V GKIVVCDRG N+RV+KG VV++AGGLGMI+ANT A GEE +AD+HL+P  
Sbjct: 387 PGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAI 446

Query: 454 AVGQKSGDAIKSYISSDSNPTATISTGTTRLGVQPSPVVAAFSSRGPNLITPQILKPDLI 513
           AVG+K+GD ++ Y+ SDS PTA +    T L V+PSPVVAAFSSRGPN +TP+ILKPD+I
Sbjct: 447 AVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVI 506

Query: 514 APGVNILAGWTGGAGPTGLDSDKRHVAFNIISGTSMSCPHISGLAALLKAAHPDWSPAAI 573
            PGVNILAGW+   GPTGLD D R   FNI+SGTSMSCPHISGLA LLKAAHP+WSP+AI
Sbjct: 507 GPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAI 566

Query: 574 RSALMTTAYSTYKNGETIQDISNGSPSTPFDIGAGHVNPTAALDPGLVYDTTTDDYLAFL 633
           +SALMTTAY        + D ++ S S P+  G+GHV+P  AL PGLVYD +T++Y+ FL
Sbjct: 567 KSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFL 626

Query: 634 CALNYSSLQIKVISKKDFTCNGNKNYKLEDLNYPSFAVPLETPSTKGGENVAPTTVKYTR 693
           C+L+Y+   I  I K+       K      LNYPSF+V        GG+ V    V+YTR
Sbjct: 627 CSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLF------GGKRV----VRYTR 686

Query: 694 TLTNKGAPST-YKVSVTAKSSSVKILVEPESLSFTEANEQKSYTVTFTASPMPS--GSQS 753
            +TN GA S+ YKV+V   + SV I V+P  LSF    E+K YTVTF +    S      
Sbjct: 687 EVTNVGAASSVYKVTVNG-APSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAE 746

Query: 754 FARLEWSDGKHIVGSPIAFTW 770
           F  + WS+ +H V SP+AF+W
Sbjct: 747 FGSITWSNPQHEVRSPVAFSW 751

BLAST of PI0004912 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 1.0e-214
Identity = 396/774 (51.16%), Postives = 518/774 (66.93%), Query Frame = 0

Query: 12  LFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSSLKSVS------ 71
           LF+I   + +F +A+ + Q   KKTY+IHMDK+ MP  + +H QWY S + SV+      
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE 71

Query: 72  ---DSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLGLG 131
              ++ ++LY+Y T  HG + +LT EEA+ +E+++G++AV PE +YELHTTR+P FLGL 
Sbjct: 72  EEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLE 131

Query: 132 KSVS--FFPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSC 191
           +  S   +   V    V++GVLDTG+WPE ESF+D G+ P+PA+W+G CE GK F   +C
Sbjct: 132 RQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNC 191

Query: 192 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 251
           NRK++GAR F +GYEAA G IDE  E KSPRD DGHG+HT+ T AGS V GANLFGFA G
Sbjct: 192 NRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYG 251

Query: 252 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNTADFYRDNVAI 311
           TARGMA +ARVA YKVCW+GGCFSSDIL+A+D++V DG  +LS+SLGG  + + RD+++I
Sbjct: 252 TARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSI 311

Query: 312 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 371
             F A   GVFVSCSAGNGGP   +L+NV+PWITTVGA T+DRDFPA V +G  +   G 
Sbjct: 312 ATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGV 371

Query: 372 SLYSGKPL--PNSLLPIVY-AASASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQK 431
           SLY G+ +   N   P+VY   +AS+    S CL   L+   V GKIV+CDRG   RVQK
Sbjct: 372 SLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQK 431

Query: 432 GLVVKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYISSDSNPTATIST 491
           G VVK AGG+GM+L NT   GEE +AD+H++P  AVG+K G  IK Y  +    TA++  
Sbjct: 432 GQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEI 491

Query: 492 GTTRLGVQPSPVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHV 551
             TR+G++PSPVVAAFSSRGPN ++ +ILKPDL+APGVNILA WTG   P+ L SD R V
Sbjct: 492 LGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRV 551

Query: 552 AFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSP 611
            FNI+SGTSMSCPH+SG+AAL+K+ HPDWSPAAI+SALMTTAY      + + D S  +P
Sbjct: 552 KFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAP 611

Query: 612 STPFDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISK-KDFTCNGNKN 671
           S+P+D GAGH++P  A DPGLVYD    +Y  FLC  + S  Q+KV +K  + TC     
Sbjct: 612 SSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLA 671

Query: 672 YKLEDLNYPSFAVPLETPSTKGGENVAPTTVKYTRTLTNKGAP-STYKVSVTAKSSSVKI 731
               +LNYP+        S    EN     +   RT+TN G   S+YKVSV +      +
Sbjct: 672 KNPGNLNYPAI-------SALFPENTHVKAMTLRRTVTNVGPHISSYKVSV-SPFKGASV 731

Query: 732 LVEPESLSFTEANEQKSYTVTFTASPMPSGSQSFARLEWSDGKHIVGSPIAFTW 770
            V+P++L+FT  +++ SYTVTF  +        F  L W    H V SP+  TW
Sbjct: 732 TVQPKTLNFTSKHQKLSYTVTF-RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of PI0004912 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 2.8e-204
Identity = 386/777 (49.68%), Postives = 512/777 (65.89%), Query Frame = 0

Query: 11  LLFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSSLKSVSDSAQ- 70
           + F+     C F+ +  S+  L  ++YI+H+ +++ P  F  H  W+ S L+S+  S Q 
Sbjct: 9   IFFVFPLLLCFFSPSSSSSDGL--ESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQP 68

Query: 71  --MLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLGLGKSVSF 130
             +LYSY+  +HGFS RL+  +   + +   +++V P+   E+HTT TP FLG  ++   
Sbjct: 69  ATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGL 128

Query: 131 FPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSCNRKLIGA 190
           +  S     VI+GVLDTG+WPE  SFSD GLGPIP++WKGECE+G +F +SSCNRKLIGA
Sbjct: 129 WSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGA 188

Query: 191 RYFSKGYEAAFGPIDE--SQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAGTARGM 250
           R F +GY        +  ++ES+SPRD +GHG+HT++TAAGS V  A+L+ +A GTA GM
Sbjct: 189 RAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGM 248

Query: 251 AAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLG--GNTADFYRDNVAIGAF 310
           A++AR+A YK+CW GGC+ SDILAAMD++V DG +++S+S+G  G+  +++ D++AIGAF
Sbjct: 249 ASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAF 308

Query: 311 SAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGESLY 370
            A   G+ VSCSAGN GP+  T +N+APWI TVGA T+DR+F A    G+GK  TG SLY
Sbjct: 309 GATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLY 368

Query: 371 SGKPLPNSLLPIVYAASASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQKGLVVKE 430
           +G+ LP+S L +VY    S      LC    LN + V GKIV+CDRGGN+RV+KG  VK 
Sbjct: 369 AGESLPDSQLSLVY----SGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKL 428

Query: 431 AGGLGMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYISSDSNPTATISTGTTRLG 490
           AGG GMILANT   GEE  AD+HL+P   VG K+GD I+ YI +  +PTA IS   T +G
Sbjct: 429 AGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIG 488

Query: 491 VQ-PSPVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFNII 550
              PSP VAAFSSRGPN +TP ILKPD+IAPGVNILAGWTG  GPT LD D R V FNII
Sbjct: 489 PSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNII 548

Query: 551 SGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTPFD 610
           SGTSMSCPH+SGLAALL+ AHPDWSPAAI+SAL+TTAY    +GE I+D++ G  S  F 
Sbjct: 549 SGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFI 608

Query: 611 IGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDF---TCNGNKNYKL 670
            GAGHV+P  AL+PGLVYD    +Y+AFLCA+ Y    I V  +       C  +K    
Sbjct: 609 HGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTA 668

Query: 671 EDLNYPSFAVPLETPSTKGGENVAPTTVKYTRTLTNKGA--PSTYKVSVTAKSSSVKILV 730
            DLNYPSF+V   +     GE      VKY R + N G+   + Y+V V +  ++V+I V
Sbjct: 669 GDLNYPSFSVVFAST----GE-----VVKYKRVVKNVGSNVDAVYEVGVKS-PANVEIDV 728

Query: 731 EPESLSFTEANEQKSYTVTFTASPMPSG-----SQSFARLEWSDGKHIVGSPIAFTW 770
            P  L+F++      Y VTF +  +  G        F  +EW+DG+H+V SP+A  W
Sbjct: 729 SPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of PI0004912 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 2.5e-200
Identity = 383/778 (49.23%), Postives = 512/778 (65.81%), Query Frame = 0

Query: 9   WFLLFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSSLKSVSDS- 68
           ++  FL++ SS        S       TYI+H+D    P  F  HF WY SSL S++ S 
Sbjct: 5   FYFFFLLTLSS-----PSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP 64

Query: 69  AQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLGLGKS--V 128
             ++++Y+TV HGFS RLT ++A  +     +++V PE    LHTTR+PEFLGL  +   
Sbjct: 65  PSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKA 124

Query: 129 SFFPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSCNRKLI 188
                S   S ++IGV+DTGVWPE  SF D GLGP+P  WKG+C   ++F  S+CNRKL+
Sbjct: 125 GLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLV 184

Query: 189 GARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAGTARGM 248
           GAR+F  GYEA  G ++E+ E +SPRD DGHG+HT++ +AG  V  A+  G+A G A GM
Sbjct: 185 GARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGM 244

Query: 249 AAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNTADFYRDNVAIGAFSA 308
           A +AR+A YKVCW  GC+ SDILAA D +V DG +++S+S+GG    +Y D +AIGAF A
Sbjct: 245 APKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGA 304

Query: 309 AAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGESLYSG 368
             +G+FVS SAGNGGP + T++NVAPW+TTVGAGT+DRDFPA V LGNGK I+G S+Y G
Sbjct: 305 IDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGG 364

Query: 369 KPL-PNSLLPIVYAAS--ASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQKGLVVK 428
             L P  + P+VY  S    +  S SLCL  +L+P  V GKIV+CDRG NSR  KG +V+
Sbjct: 365 PGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVR 424

Query: 429 EAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYIS------SDSNPTATIS 488
           + GGLGMI+AN    GE  +AD H++P  +VG   GD I+ YIS      S  +PTATI 
Sbjct: 425 KNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIV 484

Query: 489 TGTTRLGVQPSPVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRH 548
              TRLG++P+PVVA+FS+RGPN  TP+ILKPD+IAPG+NILA W    GP+G+ SD R 
Sbjct: 485 FKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRR 544

Query: 549 VAFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGS 608
             FNI+SGTSM+CPH+SGLAALLKAAHPDWSPAAIRSAL+TTAY+   +GE + D S G+
Sbjct: 545 TEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGN 604

Query: 609 PSTPFDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKN 668
            S+  D G+GHV+PT A+DPGLVYD T+ DY+ FLC  NY+   I  I+++   C+G + 
Sbjct: 605 TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARR 664

Query: 669 Y-KLEDLNYPSFAVPLETPSTKGGENVAPTTVKYTRTLTNKG-APSTYKVSVTAKSSSVK 728
              + +LNYPSF+V  +    + GE+   T   + RT+TN G + S Y++ +     +  
Sbjct: 665 AGHVGNLNYPSFSVVFQ----QYGESKMST--HFIRTVTNVGDSDSVYEIKIRPPRGTT- 724

Query: 729 ILVEPESLSFTEANEQKSYTVTF--TASPMPSGSQSF--ARLEWSDGKHIVGSPIAFT 769
           + VEPE LSF    ++ S+ V    T   +  G+ +     + WSDGK  V SP+  T
Sbjct: 725 VTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of PI0004912 vs. ExPASy TrEMBL
Match: A0A0A0LKN0 (Xylem serine proteinase 1 OS=Cucumis sativus OX=3659 GN=Csa_2G354860 PE=3 SV=1)

HSP 1 Score: 1466.4 bits (3795), Expect = 0.0e+00
Identity = 743/770 (96.49%), Postives = 755/770 (98.05%), Query Frame = 0

Query: 1   MKTCRVSQWFLLFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSS 60
           MKTCRVSQWFLLFLISF SC FTEAQKSNQQLKKKTYIIHMDK NMPQAFDDHFQWYDSS
Sbjct: 1   MKTCRVSQWFLLFLISFCSCSFTEAQKSNQQLKKKTYIIHMDKTNMPQAFDDHFQWYDSS 60

Query: 61  LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLG 120
           LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGI+AV PEMKYELHTTRTPEFLG
Sbjct: 61  LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGIIAVIPEMKYELHTTRTPEFLG 120

Query: 121 LGKSVSFFPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSC 180
           LGKSVSFFPAS KVS VIIGVLDTGVWPELESFSD GLGPIPASWKGECEVGKNFTSS+C
Sbjct: 121 LGKSVSFFPASEKVSEVIIGVLDTGVWPELESFSDAGLGPIPASWKGECEVGKNFTSSNC 180

Query: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240
           NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG
Sbjct: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240

Query: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNTADFYRDNVAI 300
           TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGN+AD+YRDNVAI
Sbjct: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNSADYYRDNVAI 300

Query: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360
           GAFSA AQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE
Sbjct: 301 GAFSATAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360

Query: 361 SLYSGKPLPNSLLPIVYAASASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQKGLV 420
           SLYSGKPLPNSLLPIV AASASNSSSGSLCLS TLNPAKV GKIVVCDRGGNSRVQKG+V
Sbjct: 361 SLYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVTGKIVVCDRGGNSRVQKGVV 420

Query: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYISSDSNPTATISTGTT 480
           VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQK+GDAIK+YISSDSNPTATISTGTT
Sbjct: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKAGDAIKNYISSDSNPTATISTGTT 480

Query: 481 RLGVQPSPVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540
           RLGVQPSPVVAAFSSRGPNL+TPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN
Sbjct: 481 RLGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540

Query: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600
           IISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYKNGE IQDISNGSPSTP
Sbjct: 541 IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEMIQDISNGSPSTP 600

Query: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660
           FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE
Sbjct: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660

Query: 661 DLNYPSFAVPLETPSTKGGENVAPTTVKYTRTLTNKGAPSTYKVSVTAKSSSVKILVEPE 720
           DLNYPSFAVPLETPST+GGENVAPTT+KYTRTLTNKGA STYKVSVTAKSSSVKI+VEPE
Sbjct: 661 DLNYPSFAVPLETPSTRGGENVAPTTIKYTRTLTNKGASSTYKVSVTAKSSSVKIVVEPE 720

Query: 721 SLSFTEANEQKSYTVTFTASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 771
           SLSFTE NEQKSYTVTF ASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT
Sbjct: 721 SLSFTEVNEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 770

BLAST of PI0004912 vs. ExPASy TrEMBL
Match: A0A1S3BA54 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103487850 PE=3 SV=1)

HSP 1 Score: 1462.6 bits (3785), Expect = 0.0e+00
Identity = 741/770 (96.23%), Postives = 754/770 (97.92%), Query Frame = 0

Query: 1   MKTCRVSQWFLLFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSS 60
           MKTCRVSQWFLLFLISFSSC FTEAQKSNQQLKKKTYIIHMDK +MPQAFDDHFQWYD+S
Sbjct: 1   MKTCRVSQWFLLFLISFSSCSFTEAQKSNQQLKKKTYIIHMDKTSMPQAFDDHFQWYDTS 60

Query: 61  LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLG 120
           LKSVSDSAQMLYSYN VIHGFST LTVEEAKLMEKQEGI+AV PEMKYELHTTRTPEFLG
Sbjct: 61  LKSVSDSAQMLYSYNNVIHGFSTSLTVEEAKLMEKQEGIIAVMPEMKYELHTTRTPEFLG 120

Query: 121 LGKSVSFFPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSC 180
           LGKS SFFPAS KVS VIIG+LDTGVWPELESFSDDGLGPIPASWKGECEVGKNF SSSC
Sbjct: 121 LGKSASFFPASAKVSEVIIGILDTGVWPELESFSDDGLGPIPASWKGECEVGKNFNSSSC 180

Query: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240
           NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG
Sbjct: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240

Query: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNTADFYRDNVAI 300
           TARGMAAEARVATYKVCWLGGCFSSDILAA+DKSVEDGCNI+S+SLGGN+AD+YRDNVAI
Sbjct: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAIDKSVEDGCNIVSMSLGGNSADYYRDNVAI 300

Query: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360
           GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE
Sbjct: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360

Query: 361 SLYSGKPLPNSLLPIVYAASASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQKGLV 420
           SLYSGKPLPNSLLPIV AASASNSSSGSLCLS TLNPAKV GKIVVCDRGGNSRVQKGLV
Sbjct: 361 SLYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVAGKIVVCDRGGNSRVQKGLV 420

Query: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYISSDSNPTATISTGTT 480
           VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQ++GDAIKSYISSDSNPTATISTGTT
Sbjct: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQEAGDAIKSYISSDSNPTATISTGTT 480

Query: 481 RLGVQPSPVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540
           RLGVQPSPVVAAFSSRGPNL+TPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN
Sbjct: 481 RLGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540

Query: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600
           IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP
Sbjct: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600

Query: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660
           FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE
Sbjct: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660

Query: 661 DLNYPSFAVPLETPSTKGGENVAPTTVKYTRTLTNKGAPSTYKVSVTAKSSSVKILVEPE 720
           DLNYPSFAVPLETPSTK GENVAPTTVKYTRTLTNKGAPSTYKVSVTAK SSVKI+V PE
Sbjct: 661 DLNYPSFAVPLETPSTKRGENVAPTTVKYTRTLTNKGAPSTYKVSVTAKISSVKIVVAPE 720

Query: 721 SLSFTEANEQKSYTVTFTASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 771
           SLSFTEANEQKSYTVTF ASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT
Sbjct: 721 SLSFTEANEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 770

BLAST of PI0004912 vs. ExPASy TrEMBL
Match: A0A5A7UYG7 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00750 PE=3 SV=1)

HSP 1 Score: 1460.7 bits (3780), Expect = 0.0e+00
Identity = 740/770 (96.10%), Postives = 752/770 (97.66%), Query Frame = 0

Query: 1   MKTCRVSQWFLLFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSS 60
           MKTCRVSQWFLLFLISFSSC FTEAQKSNQQLKKKTYIIHMDK +MPQAFDDHFQWYD+ 
Sbjct: 1   MKTCRVSQWFLLFLISFSSCSFTEAQKSNQQLKKKTYIIHMDKTSMPQAFDDHFQWYDTC 60

Query: 61  LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLG 120
           LKSVSDSAQMLYSYN VIHGFST LTVEEAKLMEKQEGI+AV PEMKYELHTTRTPEFLG
Sbjct: 61  LKSVSDSAQMLYSYNNVIHGFSTSLTVEEAKLMEKQEGIIAVMPEMKYELHTTRTPEFLG 120

Query: 121 LGKSVSFFPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSC 180
           LGKS SFFPAS KVS VIIG+LDTGVWPELESFSDDGLGPIPASWKGECEVGKNF SSSC
Sbjct: 121 LGKSASFFPASAKVSEVIIGILDTGVWPELESFSDDGLGPIPASWKGECEVGKNFNSSSC 180

Query: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240
           NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG
Sbjct: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240

Query: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNTADFYRDNVAI 300
           TARGMAAEARVATYKVCWLGGCFSSDILAA+DKSVEDGCNI+S+SLGGN+AD+YRDNVAI
Sbjct: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAIDKSVEDGCNIVSMSLGGNSADYYRDNVAI 300

Query: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360
           GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE
Sbjct: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360

Query: 361 SLYSGKPLPNSLLPIVYAASASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQKGLV 420
           SLYSGKPLPNSLLPIV AASASNSSSGSLCLS TLNPAKV GKIVVCDRGGNSRVQKGLV
Sbjct: 361 SLYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVAGKIVVCDRGGNSRVQKGLV 420

Query: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYISSDSNPTATISTGTT 480
           VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQK+GDAIKSYISSDSNPTATISTGTT
Sbjct: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKAGDAIKSYISSDSNPTATISTGTT 480

Query: 481 RLGVQPSPVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540
           RLGVQPSPVVAAFSSRGPNL+TPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN
Sbjct: 481 RLGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540

Query: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600
           IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP
Sbjct: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600

Query: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660
           FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE
Sbjct: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660

Query: 661 DLNYPSFAVPLETPSTKGGENVAPTTVKYTRTLTNKGAPSTYKVSVTAKSSSVKILVEPE 720
           DLNYPSFAVPLETPSTK GENVAPTTVKYTRTLTNKG PSTYKVSVTAK SSVKI+V PE
Sbjct: 661 DLNYPSFAVPLETPSTKRGENVAPTTVKYTRTLTNKGVPSTYKVSVTAKISSVKIVVAPE 720

Query: 721 SLSFTEANEQKSYTVTFTASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 771
           SLSFTEANEQKSYTVTF ASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT
Sbjct: 721 SLSFTEANEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 770

BLAST of PI0004912 vs. ExPASy TrEMBL
Match: A0A6J1HBB4 (subtilisin-like protease SBT1.7 OS=Cucurbita moschata OX=3662 GN=LOC111462526 PE=3 SV=1)

HSP 1 Score: 1346.3 bits (3483), Expect = 0.0e+00
Identity = 676/771 (87.68%), Postives = 720/771 (93.39%), Query Frame = 0

Query: 1   MKTCRVSQWFLLFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSS 60
           ++TCRVSQWFLLFLIS  SC F  AQKS+QQLKKKTYIIHMD+ NMPQAFDDHFQWYDSS
Sbjct: 3   IQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSS 62

Query: 61  LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLG 120
           LKSVSDSAQ LY YNTV+HGFSTRLTVEEAKL+EKQEGILAV PE+KY+LHTTRTPEFLG
Sbjct: 63  LKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEFLG 122

Query: 121 LGKSVSFFPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSC 180
           L KSVSFFPASVKV  VI+GVLDTGV PELESF D GLGP+P SWKGECEVGKNF+SSSC
Sbjct: 123 LEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSSSC 182

Query: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240
           NRKLIGARYFSKGYEAAFG IDESQESKSPRDDDGHGSHTSTTAAGSAVTGA+LFGF AG
Sbjct: 183 NRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFGAG 242

Query: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNTADFYRDNVAI 300
           TA+GMAAEARVATYKVCWLGGCF SDILAAMDK++EDG N+LS+SLGG++ D+YRDNVAI
Sbjct: 243 TAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNVAI 302

Query: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360
           GAFSA AQGVFVSCSAGNGGPSS +LSNVAPWITTVGAGTLDRDFP YVTLGNGKK TG+
Sbjct: 303 GAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFTGQ 362

Query: 361 SLYSGKPLPNSLLPIVYAASASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQKGLV 420
           SLY+GKPL +SL+PIVYAA ASNS+SGSLCL+STLNPAKV GKIVVCDRGGNSRVQKGLV
Sbjct: 363 SLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGLV 422

Query: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYISSDSNPTATISTGTT 480
           VK+AGG GMILANTE YGEEQLADAHL+P AAVGQK+GDAIKSYIS+++NPTATIS G+T
Sbjct: 423 VKDAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAGST 482

Query: 481 RLGVQPSPVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540
           RLGVQPSP+VAAFSSRGPNL+TPQILKPDLIAPGVNILAGWT G GPTGLDSDKRHVAFN
Sbjct: 483 RLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTSGVGPTGLDSDKRHVAFN 542

Query: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600
           IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYK GE IQD+S+G PSTP
Sbjct: 543 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPSTP 602

Query: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660
           FDIGAGHVNPTAALDPGLVYD T +DY AFLCALNYSS QIKVISKKDFTC+GNKNYKLE
Sbjct: 603 FDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYKLE 662

Query: 661 DLNYPSFAVPLETPSTKGG-ENVAPTTVKYTRTLTNKGAPSTYKVSVTAKSSSVKILVEP 720
           DLNYPSFAV LETPSTKGG E  APTTVKYTRTLTNKGAPSTYKVSVT+KS SVKI+VEP
Sbjct: 663 DLNYPSFAVALETPSTKGGAEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMVEP 722

Query: 721 ESLSFTEANEQKSYTVTFTASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 771
           ESLSF + NEQKSYTVTF AS MPSGS+SFARLEWSDGKH VGSPIAFTWT
Sbjct: 723 ESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 773

BLAST of PI0004912 vs. ExPASy TrEMBL
Match: A0A6J1BPX8 (subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111004737 PE=3 SV=1)

HSP 1 Score: 1338.9 bits (3464), Expect = 0.0e+00
Identity = 669/770 (86.88%), Postives = 721/770 (93.64%), Query Frame = 0

Query: 1   MKTCRVSQWFLLFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSS 60
           M+TCRVS+ FLLFLI F S  +  A+K+NQ+LKKKTYIIHMD+ NMPQAFDDHFQWYDSS
Sbjct: 6   MQTCRVSRLFLLFLIVFYSYSYVGAEKTNQKLKKKTYIIHMDRTNMPQAFDDHFQWYDSS 65

Query: 61  LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLG 120
           LKSVSDSAQMLYSY+TVIHGFSTRLTVEEA+LMEK+EGILAV PE KYELHTTRTPEFLG
Sbjct: 66  LKSVSDSAQMLYSYSTVIHGFSTRLTVEEAELMEKREGILAVIPEAKYELHTTRTPEFLG 125

Query: 121 LGKSVSFFPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSC 180
           LGKS SFFPASVKV  VIIG+LDTGVWPELESF+D GLGP+P SWKGECEVGKNF+SSSC
Sbjct: 126 LGKSDSFFPASVKVGEVIIGILDTGVWPELESFNDAGLGPVPTSWKGECEVGKNFSSSSC 185

Query: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240
           NRKLIGARYF+KGYE+AFGPIDESQESKSP+DDDGHG+HTSTTAAGSAVTGANLFG+A+G
Sbjct: 186 NRKLIGARYFAKGYESAFGPIDESQESKSPKDDDGHGTHTSTTAAGSAVTGANLFGYASG 245

Query: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNTADFYRDNVAI 300
           TARGMAA+ARVATYKVCWLGGCF SDILAA+DK+VEDG N+LS+SLGG++ D+YRDNVAI
Sbjct: 246 TARGMAAQARVATYKVCWLGGCFGSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAI 305

Query: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360
           GAFSAAAQGVFVSCSAGNGGPSS +LSNVAPWITTVGAGTLDRDFPAY+TLGNGKK TGE
Sbjct: 306 GAFSAAAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPAYITLGNGKKFTGE 365

Query: 361 SLYSGKPLPNSLLPIVYAASASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQKGLV 420
           SLYSGKPLP+SL+PIVYAA ASNS+SGS CL+ TLNPAKV GKIVVCDRGGNSRVQKG+V
Sbjct: 366 SLYSGKPLPDSLVPIVYAAHASNSTSGSSCLTGTLNPAKVAGKIVVCDRGGNSRVQKGVV 425

Query: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYISSDSNPTATISTGTT 480
           VKEAGG GMILANTE YGEE+LADAHL+P AAVGQK+GDAIKSYISSD+NPTA  STGTT
Sbjct: 426 VKEAGGAGMILANTETYGEEELADAHLLPAAAVGQKTGDAIKSYISSDANPTAKFSTGTT 485

Query: 481 RLGVQPSPVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540
           RLGVQPSPVVAAFSSRGPNL+TP ILKPDLIAPGVNILAGWTG AGPTGLD DKR VAFN
Sbjct: 486 RLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGAAGPTGLDGDKRRVAFN 545

Query: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600
           IISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYKNGE IQDIS+G PSTP
Sbjct: 546 IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEAIQDISSGLPSTP 605

Query: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660
           FDIGAGHVNP AALDPGLVYDTTTDDY  FLCALNY+SLQIKVI+KKDFTC+ +KNYKLE
Sbjct: 606 FDIGAGHVNPGAALDPGLVYDTTTDDYFVFLCALNYTSLQIKVIAKKDFTCSASKNYKLE 665

Query: 661 DLNYPSFAVPLETPSTKGGENVAPTTVKYTRTLTNKGAPSTYKVSVTAKSSSVKILVEPE 720
           DLNYPSFAVPLETPS +GG + APTTVKYTRTLTNK A STYKVS T+KS SVKI+VEPE
Sbjct: 666 DLNYPSFAVPLETPSVRGGGDAAPTTVKYTRTLTNKAASSTYKVSETSKSPSVKIVVEPE 725

Query: 721 SLSFTEANEQKSYTVTFTASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 771
           SLSF E NEQKSYTVTF ASPMPSG++SFARLEWSDGKHIVGSPIAFTWT
Sbjct: 726 SLSFAEENEQKSYTVTFIASPMPSGTESFARLEWSDGKHIVGSPIAFTWT 775

BLAST of PI0004912 vs. NCBI nr
Match: XP_004142884.1 (subtilisin-like protease SBT1.7 [Cucumis sativus] >KGN62460.1 hypothetical protein Csa_018794 [Cucumis sativus])

HSP 1 Score: 1466.4 bits (3795), Expect = 0.0e+00
Identity = 743/770 (96.49%), Postives = 755/770 (98.05%), Query Frame = 0

Query: 1   MKTCRVSQWFLLFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSS 60
           MKTCRVSQWFLLFLISF SC FTEAQKSNQQLKKKTYIIHMDK NMPQAFDDHFQWYDSS
Sbjct: 1   MKTCRVSQWFLLFLISFCSCSFTEAQKSNQQLKKKTYIIHMDKTNMPQAFDDHFQWYDSS 60

Query: 61  LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLG 120
           LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGI+AV PEMKYELHTTRTPEFLG
Sbjct: 61  LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGIIAVIPEMKYELHTTRTPEFLG 120

Query: 121 LGKSVSFFPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSC 180
           LGKSVSFFPAS KVS VIIGVLDTGVWPELESFSD GLGPIPASWKGECEVGKNFTSS+C
Sbjct: 121 LGKSVSFFPASEKVSEVIIGVLDTGVWPELESFSDAGLGPIPASWKGECEVGKNFTSSNC 180

Query: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240
           NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG
Sbjct: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240

Query: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNTADFYRDNVAI 300
           TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGN+AD+YRDNVAI
Sbjct: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNSADYYRDNVAI 300

Query: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360
           GAFSA AQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE
Sbjct: 301 GAFSATAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360

Query: 361 SLYSGKPLPNSLLPIVYAASASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQKGLV 420
           SLYSGKPLPNSLLPIV AASASNSSSGSLCLS TLNPAKV GKIVVCDRGGNSRVQKG+V
Sbjct: 361 SLYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVTGKIVVCDRGGNSRVQKGVV 420

Query: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYISSDSNPTATISTGTT 480
           VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQK+GDAIK+YISSDSNPTATISTGTT
Sbjct: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKAGDAIKNYISSDSNPTATISTGTT 480

Query: 481 RLGVQPSPVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540
           RLGVQPSPVVAAFSSRGPNL+TPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN
Sbjct: 481 RLGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540

Query: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600
           IISGTSMSCPHISGLAAL+KAAHPDWSPAAIRSALMTTAYSTYKNGE IQDISNGSPSTP
Sbjct: 541 IISGTSMSCPHISGLAALVKAAHPDWSPAAIRSALMTTAYSTYKNGEMIQDISNGSPSTP 600

Query: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660
           FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE
Sbjct: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660

Query: 661 DLNYPSFAVPLETPSTKGGENVAPTTVKYTRTLTNKGAPSTYKVSVTAKSSSVKILVEPE 720
           DLNYPSFAVPLETPST+GGENVAPTT+KYTRTLTNKGA STYKVSVTAKSSSVKI+VEPE
Sbjct: 661 DLNYPSFAVPLETPSTRGGENVAPTTIKYTRTLTNKGASSTYKVSVTAKSSSVKIVVEPE 720

Query: 721 SLSFTEANEQKSYTVTFTASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 771
           SLSFTE NEQKSYTVTF ASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT
Sbjct: 721 SLSFTEVNEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 770

BLAST of PI0004912 vs. NCBI nr
Match: XP_008444575.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])

HSP 1 Score: 1462.6 bits (3785), Expect = 0.0e+00
Identity = 741/770 (96.23%), Postives = 754/770 (97.92%), Query Frame = 0

Query: 1   MKTCRVSQWFLLFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSS 60
           MKTCRVSQWFLLFLISFSSC FTEAQKSNQQLKKKTYIIHMDK +MPQAFDDHFQWYD+S
Sbjct: 1   MKTCRVSQWFLLFLISFSSCSFTEAQKSNQQLKKKTYIIHMDKTSMPQAFDDHFQWYDTS 60

Query: 61  LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLG 120
           LKSVSDSAQMLYSYN VIHGFST LTVEEAKLMEKQEGI+AV PEMKYELHTTRTPEFLG
Sbjct: 61  LKSVSDSAQMLYSYNNVIHGFSTSLTVEEAKLMEKQEGIIAVMPEMKYELHTTRTPEFLG 120

Query: 121 LGKSVSFFPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSC 180
           LGKS SFFPAS KVS VIIG+LDTGVWPELESFSDDGLGPIPASWKGECEVGKNF SSSC
Sbjct: 121 LGKSASFFPASAKVSEVIIGILDTGVWPELESFSDDGLGPIPASWKGECEVGKNFNSSSC 180

Query: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240
           NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG
Sbjct: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240

Query: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNTADFYRDNVAI 300
           TARGMAAEARVATYKVCWLGGCFSSDILAA+DKSVEDGCNI+S+SLGGN+AD+YRDNVAI
Sbjct: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAIDKSVEDGCNIVSMSLGGNSADYYRDNVAI 300

Query: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360
           GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE
Sbjct: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360

Query: 361 SLYSGKPLPNSLLPIVYAASASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQKGLV 420
           SLYSGKPLPNSLLPIV AASASNSSSGSLCLS TLNPAKV GKIVVCDRGGNSRVQKGLV
Sbjct: 361 SLYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVAGKIVVCDRGGNSRVQKGLV 420

Query: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYISSDSNPTATISTGTT 480
           VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQ++GDAIKSYISSDSNPTATISTGTT
Sbjct: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQEAGDAIKSYISSDSNPTATISTGTT 480

Query: 481 RLGVQPSPVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540
           RLGVQPSPVVAAFSSRGPNL+TPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN
Sbjct: 481 RLGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540

Query: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600
           IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP
Sbjct: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600

Query: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660
           FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE
Sbjct: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660

Query: 661 DLNYPSFAVPLETPSTKGGENVAPTTVKYTRTLTNKGAPSTYKVSVTAKSSSVKILVEPE 720
           DLNYPSFAVPLETPSTK GENVAPTTVKYTRTLTNKGAPSTYKVSVTAK SSVKI+V PE
Sbjct: 661 DLNYPSFAVPLETPSTKRGENVAPTTVKYTRTLTNKGAPSTYKVSVTAKISSVKIVVAPE 720

Query: 721 SLSFTEANEQKSYTVTFTASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 771
           SLSFTEANEQKSYTVTF ASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT
Sbjct: 721 SLSFTEANEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 770

BLAST of PI0004912 vs. NCBI nr
Match: KAA0060942.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 1460.7 bits (3780), Expect = 0.0e+00
Identity = 740/770 (96.10%), Postives = 752/770 (97.66%), Query Frame = 0

Query: 1   MKTCRVSQWFLLFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSS 60
           MKTCRVSQWFLLFLISFSSC FTEAQKSNQQLKKKTYIIHMDK +MPQAFDDHFQWYD+ 
Sbjct: 1   MKTCRVSQWFLLFLISFSSCSFTEAQKSNQQLKKKTYIIHMDKTSMPQAFDDHFQWYDTC 60

Query: 61  LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLG 120
           LKSVSDSAQMLYSYN VIHGFST LTVEEAKLMEKQEGI+AV PEMKYELHTTRTPEFLG
Sbjct: 61  LKSVSDSAQMLYSYNNVIHGFSTSLTVEEAKLMEKQEGIIAVMPEMKYELHTTRTPEFLG 120

Query: 121 LGKSVSFFPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSC 180
           LGKS SFFPAS KVS VIIG+LDTGVWPELESFSDDGLGPIPASWKGECEVGKNF SSSC
Sbjct: 121 LGKSASFFPASAKVSEVIIGILDTGVWPELESFSDDGLGPIPASWKGECEVGKNFNSSSC 180

Query: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240
           NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG
Sbjct: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240

Query: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNTADFYRDNVAI 300
           TARGMAAEARVATYKVCWLGGCFSSDILAA+DKSVEDGCNI+S+SLGGN+AD+YRDNVAI
Sbjct: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAIDKSVEDGCNIVSMSLGGNSADYYRDNVAI 300

Query: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360
           GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE
Sbjct: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360

Query: 361 SLYSGKPLPNSLLPIVYAASASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQKGLV 420
           SLYSGKPLPNSLLPIV AASASNSSSGSLCLS TLNPAKV GKIVVCDRGGNSRVQKGLV
Sbjct: 361 SLYSGKPLPNSLLPIVSAASASNSSSGSLCLSGTLNPAKVAGKIVVCDRGGNSRVQKGLV 420

Query: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYISSDSNPTATISTGTT 480
           VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQK+GDAIKSYISSDSNPTATISTGTT
Sbjct: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKAGDAIKSYISSDSNPTATISTGTT 480

Query: 481 RLGVQPSPVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540
           RLGVQPSPVVAAFSSRGPNL+TPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN
Sbjct: 481 RLGVQPSPVVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540

Query: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600
           IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP
Sbjct: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600

Query: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660
           FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE
Sbjct: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660

Query: 661 DLNYPSFAVPLETPSTKGGENVAPTTVKYTRTLTNKGAPSTYKVSVTAKSSSVKILVEPE 720
           DLNYPSFAVPLETPSTK GENVAPTTVKYTRTLTNKG PSTYKVSVTAK SSVKI+V PE
Sbjct: 661 DLNYPSFAVPLETPSTKRGENVAPTTVKYTRTLTNKGVPSTYKVSVTAKISSVKIVVAPE 720

Query: 721 SLSFTEANEQKSYTVTFTASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 771
           SLSFTEANEQKSYTVTF ASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT
Sbjct: 721 SLSFTEANEQKSYTVTFIASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 770

BLAST of PI0004912 vs. NCBI nr
Match: XP_038885098.1 (subtilisin-like protease SBT1.7 [Benincasa hispida])

HSP 1 Score: 1419.1 bits (3672), Expect = 0.0e+00
Identity = 714/770 (92.73%), Postives = 747/770 (97.01%), Query Frame = 0

Query: 1   MKTCRVSQWFLLFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSS 60
           M+TCR+SQWFLLFLISF SC FTEAQKSN+QLKKKTY+IHMD+ NMPQAFDDHF+WYDSS
Sbjct: 3   MQTCRLSQWFLLFLISFCSCSFTEAQKSNRQLKKKTYVIHMDRTNMPQAFDDHFKWYDSS 62

Query: 61  LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLG 120
           LKSVS+SAQMLYSYNTV+HGFSTRLTVEEAKL+EKQEGILAV PE+KYELHTTRTPEFLG
Sbjct: 63  LKSVSNSAQMLYSYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPEVKYELHTTRTPEFLG 122

Query: 121 LGKSVSFFPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSC 180
           LGKSVSFFPAS KVS VIIGVLDTGVWPELESF+D GLGP+PASWKGECEVGKNFTSSSC
Sbjct: 123 LGKSVSFFPASEKVSEVIIGVLDTGVWPELESFNDAGLGPVPASWKGECEVGKNFTSSSC 182

Query: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240
           NRKLIGARYFSKGYEAAFG IDESQESKSPRDDDGHGSHTSTTAAGSAVTGA+LFGFAAG
Sbjct: 183 NRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFAAG 242

Query: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNTADFYRDNVAI 300
           TARGMAAEARVATYKVCWLGGCFSSDILAA+DK+VEDG N+LS+SLGG++ D+YRDNVAI
Sbjct: 243 TARGMAAEARVATYKVCWLGGCFSSDILAAIDKAVEDGVNVLSLSLGGSSPDYYRDNVAI 302

Query: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360
           GAFSAAAQGVFVSCSAGNGGPSS TLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE
Sbjct: 303 GAFSAAAQGVFVSCSAGNGGPSSGTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 362

Query: 361 SLYSGKPLPNSLLPIVYAASASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQKGLV 420
           SLYSGKPLP+SLLPIVYA +ASNS+SGSLCLSSTLNPAKV GKIVVCDRGGNSRVQKGLV
Sbjct: 363 SLYSGKPLPDSLLPIVYAGNASNSTSGSLCLSSTLNPAKVAGKIVVCDRGGNSRVQKGLV 422

Query: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYISSDSNPTATISTGTT 480
           VKEAGG+GMILANTEAYGEEQLADAHL PTAAVG+KSGDAIKSYISSD+NPTATISTGTT
Sbjct: 423 VKEAGGVGMILANTEAYGEEQLADAHLTPTAAVGKKSGDAIKSYISSDANPTATISTGTT 482

Query: 481 RLGVQPSPVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540
           RLGVQPSPVVAAFSSRGPNL+TP ILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN
Sbjct: 483 RLGVQPSPVVAAFSSRGPNLLTPHILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 542

Query: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600
           IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGE IQD+SNGSPSTP
Sbjct: 543 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGEVIQDVSNGSPSTP 602

Query: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660
           FDIGAGHVNPTAALDPGLVYDTTTDDY AFLCALNYSSLQIKVISK+DFTCNGNKNYKLE
Sbjct: 603 FDIGAGHVNPTAALDPGLVYDTTTDDYFAFLCALNYSSLQIKVISKRDFTCNGNKNYKLE 662

Query: 661 DLNYPSFAVPLETPSTKGGENVAPTTVKYTRTLTNKGAPSTYKVSVTAKSSSVKILVEPE 720
           DLNYPSFAVPLETPST+GGE+VAPTTVKYTRTLTNKGAPSTYKVSVTA    VKI+VEPE
Sbjct: 663 DLNYPSFAVPLETPSTRGGEDVAPTTVKYTRTLTNKGAPSTYKVSVTA-PKEVKIVVEPE 722

Query: 721 SLSFTEANEQKSYTVTFTASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 771
           SLSF EANEQKSYTVTF ASPMPSGS+SFARLEWSDGKHIVGSPIAFTWT
Sbjct: 723 SLSFAEANEQKSYTVTFIASPMPSGSESFARLEWSDGKHIVGSPIAFTWT 771

BLAST of PI0004912 vs. NCBI nr
Match: KAG7029314.1 (Subtilisin-like protease SBT1.7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1350.9 bits (3495), Expect = 0.0e+00
Identity = 679/771 (88.07%), Postives = 721/771 (93.51%), Query Frame = 0

Query: 1   MKTCRVSQWFLLFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSS 60
           ++TCRVSQWFLLFLIS  SC F  AQKS+QQLKKKTYIIHMD+ NMPQAFDDHFQWYDSS
Sbjct: 3   IQTCRVSQWFLLFLISSCSCSFAAAQKSSQQLKKKTYIIHMDRTNMPQAFDDHFQWYDSS 62

Query: 61  LKSVSDSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLG 120
           LKSVSDSAQ LY YNTV+HGFSTRLTVEEAKL+EKQEGILAV PE+KY+LHTTRTPEFLG
Sbjct: 63  LKSVSDSAQTLYYYNTVVHGFSTRLTVEEAKLIEKQEGILAVIPELKYDLHTTRTPEFLG 122

Query: 121 LGKSVSFFPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSC 180
           L KSVSFFPASVKV  VI+GVLDTGV PELESF D GLGP+P SWKGECEVGKNF+SSSC
Sbjct: 123 LEKSVSFFPASVKVGEVIVGVLDTGVCPELESFDDTGLGPVPVSWKGECEVGKNFSSSSC 182

Query: 181 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 240
           NRKLIGARYFSKGYEAAFG IDESQESKSPRDDDGHGSHTSTTAAGSAVTGA+LFGFAAG
Sbjct: 183 NRKLIGARYFSKGYEAAFGAIDESQESKSPRDDDGHGSHTSTTAAGSAVTGASLFGFAAG 242

Query: 241 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNTADFYRDNVAI 300
           TA+GMAAEARVATYKVCWLGGCF SDILAAMDK++EDG N+LS+SLGG++ D+YRDNVAI
Sbjct: 243 TAKGMAAEARVATYKVCWLGGCFGSDILAAMDKAIEDGVNVLSLSLGGSSPDYYRDNVAI 302

Query: 301 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 360
           GAFSA AQGVFVSCSAGNGGPSS +LSNVAPWITTVGAGTLDRDFP YVTLGNGKK TG+
Sbjct: 303 GAFSATAQGVFVSCSAGNGGPSSGSLSNVAPWITTVGAGTLDRDFPVYVTLGNGKKFTGQ 362

Query: 361 SLYSGKPLPNSLLPIVYAASASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQKGLV 420
           SLY+GKPL +SL+PIVYAA ASNS+SGSLCL+STLNPAKV GKIVVCDRGGNSRVQKGLV
Sbjct: 363 SLYNGKPLSDSLIPIVYAAHASNSTSGSLCLTSTLNPAKVAGKIVVCDRGGNSRVQKGLV 422

Query: 421 VKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYISSDSNPTATISTGTT 480
           VKEAGG GMILANTE YGEEQLADAHL+P AAVGQK+GDAIKSYIS+++NPTATIS GTT
Sbjct: 423 VKEAGGAGMILANTETYGEEQLADAHLLPAAAVGQKTGDAIKSYISTNANPTATISAGTT 482

Query: 481 RLGVQPSPVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFN 540
           RLGVQPSP+VAAFSSRGPNL+TPQILKPDLIAPGVNILAGW GG GPTGLDSDKRHVAFN
Sbjct: 483 RLGVQPSPLVAAFSSRGPNLLTPQILKPDLIAPGVNILAGWAGGVGPTGLDSDKRHVAFN 542

Query: 541 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTP 600
           IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYK GE IQD+S+G PSTP
Sbjct: 543 IISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKTGEAIQDVSSGLPSTP 602

Query: 601 FDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLE 660
           FDIGAGHVNPTAALDPGLVYD T +DY AFLCALNYSS QIKVISKKDFTC+GNKNYKLE
Sbjct: 603 FDIGAGHVNPTAALDPGLVYDATIEDYFAFLCALNYSSFQIKVISKKDFTCSGNKNYKLE 662

Query: 661 DLNYPSFAVPLETPSTK-GGENVAPTTVKYTRTLTNKGAPSTYKVSVTAKSSSVKILVEP 720
           DLNYPSFAV LETPSTK GGE  APTTVKYTRTLTNKGAPSTYKVSVT+KS SVKI+VEP
Sbjct: 663 DLNYPSFAVALETPSTKGGGEGTAPTTVKYTRTLTNKGAPSTYKVSVTSKSPSVKIMVEP 722

Query: 721 ESLSFTEANEQKSYTVTFTASPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 771
           ESLSF + NEQKSYTVTF AS MPSGS+SFARLEWSDGKH VGSPIAFTWT
Sbjct: 723 ESLSFAKPNEQKSYTVTFVASAMPSGSESFARLEWSDGKHNVGSPIAFTWT 773

BLAST of PI0004912 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 988.8 bits (2555), Expect = 2.5e-288
Identity = 494/764 (64.66%), Postives = 604/764 (79.06%), Query Frame = 0

Query: 9   WFLLFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSSLKSVSDSA 68
           +FLL  + F          S+    + TYI+HM K+ MP +FD H  WYDSSL+S+SDSA
Sbjct: 11  FFLLLCLGF-------CHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA 70

Query: 69  QMLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLGLGK-SVSF 128
           ++LY+Y   IHGFSTRLT EEA  +  Q G+++V PE +YELHTTRTP FLGL + +   
Sbjct: 71  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 130

Query: 129 FPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSCNRKLIGA 188
           FP +   S V++GVLDTGVWPE +S+SD+G GPIP+SWKG CE G NFT+S CNRKLIGA
Sbjct: 131 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 190

Query: 189 RYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAGTARGMAA 248
           R+F++GYE+  GPIDES+ES+SPRDDDGHG+HTS+TAAGS V GA+L G+A+GTARGMA 
Sbjct: 191 RFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAP 250

Query: 249 EARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNTADFYRDNVAIGAFSAAA 308
            ARVA YKVCWLGGCFSSDILAA+DK++ D  N+LS+SLGG  +D+YRD VAIGAF+A  
Sbjct: 251 RARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAME 310

Query: 309 QGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGESLYSGKP 368
           +G+ VSCSAGN GPSSS+LSNVAPWITTVGAGTLDRDFPA   LGNGK  TG SL+ G+ 
Sbjct: 311 RGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEA 370

Query: 369 LPNSLLPIVYAASASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQKGLVVKEAGGL 428
           LP+ LLP +YA +ASN+++G+LC++ TL P KV GKIV+CDRG N+RVQKG VVK AGG+
Sbjct: 371 LPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGV 430

Query: 429 GMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYISSDSNPTATISTGTTRLGVQPS 488
           GMILANT A GEE +ADAHL+P   VG+K+GD I+ Y+++D NPTA+IS   T +GV+PS
Sbjct: 431 GMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPS 490

Query: 489 PVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFNIISGTSM 548
           PVVAAFSSRGPN ITP ILKPDLIAPGVNILA WTG AGPTGL SD R V FNIISGTSM
Sbjct: 491 PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSM 550

Query: 549 SCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTPFDIGAGH 608
           SCPH+SGLAALLK+ HP+WSPAAIRSALMTTAY TYK+G+ + DI+ G PSTPFD GAGH
Sbjct: 551 SCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGH 610

Query: 609 VNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKNYKLEDLNYPSF 668
           V+PT A +PGL+YD TT+DYL FLCALNY+S QI+ +S++++TC+ +K+Y + DLNYPSF
Sbjct: 611 VSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSF 670

Query: 669 AVPLETPSTKGGENVAPTTVKYTRTLTNKGAPSTYKVSVTAKSSSVKILVEPESLSFTEA 728
           AV ++               KYTRT+T+ G   TY V VT++++ VKI VEP  L+F EA
Sbjct: 671 AVNVD----------GVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEA 730

Query: 729 NEQKSYTVTFTA-SPMPSGSQSFARLEWSDGKHIVGSPIAFTWT 771
           NE+KSYTVTFT  S  PSGS SF  +EWSDGKH+VGSP+A +WT
Sbjct: 731 NEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of PI0004912 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 765.8 bits (1976), Expect = 3.4e-221
Identity = 414/741 (55.87%), Postives = 515/741 (69.50%), Query Frame = 0

Query: 34  KKTYIIHMDKANMPQAFDDHFQWYDSSLKSVSDSAQMLYSYNTVIHGFSTRLTVEEA-KL 93
           KKTYII ++ ++ P++F  H  WY S L S S    +LY+Y T  HGFS  L   EA  L
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTTSFHGFSAYLDSTEADSL 86

Query: 94  MEKQEGILAVTPEMKYELHTTRTPEFLGLGKSVSFFPASVKVSGVIIGVLDTGVWPELES 153
           +     IL +  +  Y LHTTRTPEFLGL             +GVIIGVLDTGVWPE  S
Sbjct: 87  LSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESRS 146

Query: 154 FSDDGLGPIPASWKGECEVGKNFTSSSCNRKLIGARYFSKGYE-AAFGPIDESQESKSPR 213
           F D  +  IP+ WKGECE G +F S  CN+KLIGAR FSKG++ A+ G     +ES SPR
Sbjct: 147 FDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSPR 206

Query: 214 DDDGHGSHTSTTAAGSAVTGANLFGFAAGTARGMAAEARVATYKVCWLGGCFSSDILAAM 273
           D DGHG+HTSTTAAGSAV  A+  G+AAGTARGMA  ARVATYKVCW  GCF SDILAAM
Sbjct: 207 DVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAM 266

Query: 274 DKSVEDGCNILSVSLGGNTADFYRDNVAIGAFSAAAQGVFVSCSAGNGGPSSSTLSNVAP 333
           D+++ DG ++LS+SLGG +A +YRD +AIGAFSA  +GVFVSCSAGN GP+ ++++NVAP
Sbjct: 267 DRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAP 326

Query: 334 WITTVGAGTLDRDFPAYVTLGNGKKITGESLYSGKPLPNSLLPIVYAASASNSSSGSLCL 393
           W+ TVGAGTLDRDFPA+  LGNGK++TG SLYSG  +    L +VY  +  NSSS +LCL
Sbjct: 327 WVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY--NKGNSSSSNLCL 386

Query: 394 SSTLNPAKVVGKIVVCDRGGNSRVQKGLVVKEAGGLGMILANTEAYGEEQLADAHLIPTA 453
             +L+ + V GKIVVCDRG N+RV+KG VV++AGGLGMI+ANT A GEE +AD+HL+P  
Sbjct: 387 PGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAI 446

Query: 454 AVGQKSGDAIKSYISSDSNPTATISTGTTRLGVQPSPVVAAFSSRGPNLITPQILKPDLI 513
           AVG+K+GD ++ Y+ SDS PTA +    T L V+PSPVVAAFSSRGPN +TP+ILKPD+I
Sbjct: 447 AVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVI 506

Query: 514 APGVNILAGWTGGAGPTGLDSDKRHVAFNIISGTSMSCPHISGLAALLKAAHPDWSPAAI 573
            PGVNILAGW+   GPTGLD D R   FNI+SGTSMSCPHISGLA LLKAAHP+WSP+AI
Sbjct: 507 GPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAI 566

Query: 574 RSALMTTAYSTYKNGETIQDISNGSPSTPFDIGAGHVNPTAALDPGLVYDTTTDDYLAFL 633
           +SALMTTAY        + D ++ S S P+  G+GHV+P  AL PGLVYD +T++Y+ FL
Sbjct: 567 KSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFL 626

Query: 634 CALNYSSLQIKVISKKDFTCNGNKNYKLEDLNYPSFAVPLETPSTKGGENVAPTTVKYTR 693
           C+L+Y+   I  I K+       K      LNYPSF+V        GG+ V    V+YTR
Sbjct: 627 CSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVLF------GGKRV----VRYTR 686

Query: 694 TLTNKGAPST-YKVSVTAKSSSVKILVEPESLSFTEANEQKSYTVTFTASPMPS--GSQS 753
            +TN GA S+ YKV+V   + SV I V+P  LSF    E+K YTVTF +    S      
Sbjct: 687 EVTNVGAASSVYKVTVNG-APSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAE 746

Query: 754 FARLEWSDGKHIVGSPIAFTW 770
           F  + WS+ +H V SP+AF+W
Sbjct: 747 FGSITWSNPQHEVRSPVAFSW 751

BLAST of PI0004912 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 748.0 bits (1930), Expect = 7.3e-216
Identity = 396/774 (51.16%), Postives = 518/774 (66.93%), Query Frame = 0

Query: 12  LFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSSLKSVS------ 71
           LF+I   + +F +A+ + Q   KKTY+IHMDK+ MP  + +H QWY S + SV+      
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE 71

Query: 72  ---DSAQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLGLG 131
              ++ ++LY+Y T  HG + +LT EEA+ +E+++G++AV PE +YELHTTR+P FLGL 
Sbjct: 72  EEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLE 131

Query: 132 KSVS--FFPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSC 191
           +  S   +   V    V++GVLDTG+WPE ESF+D G+ P+PA+W+G CE GK F   +C
Sbjct: 132 RQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNC 191

Query: 192 NRKLIGARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAG 251
           NRK++GAR F +GYEAA G IDE  E KSPRD DGHG+HT+ T AGS V GANLFGFA G
Sbjct: 192 NRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYG 251

Query: 252 TARGMAAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNTADFYRDNVAI 311
           TARGMA +ARVA YKVCW+GGCFSSDIL+A+D++V DG  +LS+SLGG  + + RD+++I
Sbjct: 252 TARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSI 311

Query: 312 GAFSAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGE 371
             F A   GVFVSCSAGNGGP   +L+NV+PWITTVGA T+DRDFPA V +G  +   G 
Sbjct: 312 ATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGV 371

Query: 372 SLYSGKPL--PNSLLPIVY-AASASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQK 431
           SLY G+ +   N   P+VY   +AS+    S CL   L+   V GKIV+CDRG   RVQK
Sbjct: 372 SLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQK 431

Query: 432 GLVVKEAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYISSDSNPTATIST 491
           G VVK AGG+GM+L NT   GEE +AD+H++P  AVG+K G  IK Y  +    TA++  
Sbjct: 432 GQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEI 491

Query: 492 GTTRLGVQPSPVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHV 551
             TR+G++PSPVVAAFSSRGPN ++ +ILKPDL+APGVNILA WTG   P+ L SD R V
Sbjct: 492 LGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRV 551

Query: 552 AFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSP 611
            FNI+SGTSMSCPH+SG+AAL+K+ HPDWSPAAI+SALMTTAY      + + D S  +P
Sbjct: 552 KFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAP 611

Query: 612 STPFDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISK-KDFTCNGNKN 671
           S+P+D GAGH++P  A DPGLVYD    +Y  FLC  + S  Q+KV +K  + TC     
Sbjct: 612 SSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLA 671

Query: 672 YKLEDLNYPSFAVPLETPSTKGGENVAPTTVKYTRTLTNKGAP-STYKVSVTAKSSSVKI 731
               +LNYP+        S    EN     +   RT+TN G   S+YKVSV +      +
Sbjct: 672 KNPGNLNYPAI-------SALFPENTHVKAMTLRRTVTNVGPHISSYKVSV-SPFKGASV 731

Query: 732 LVEPESLSFTEANEQKSYTVTFTASPMPSGSQSFARLEWSDGKHIVGSPIAFTW 770
            V+P++L+FT  +++ SYTVTF  +        F  L W    H V SP+  TW
Sbjct: 732 TVQPKTLNFTSKHQKLSYTVTF-RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of PI0004912 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 713.4 bits (1840), Expect = 2.0e-205
Identity = 386/777 (49.68%), Postives = 512/777 (65.89%), Query Frame = 0

Query: 11  LLFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSSLKSVSDSAQ- 70
           + F+     C F+ +  S+  L  ++YI+H+ +++ P  F  H  W+ S L+S+  S Q 
Sbjct: 9   IFFVFPLLLCFFSPSSSSSDGL--ESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQP 68

Query: 71  --MLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLGLGKSVSF 130
             +LYSY+  +HGFS RL+  +   + +   +++V P+   E+HTT TP FLG  ++   
Sbjct: 69  ATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGL 128

Query: 131 FPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSCNRKLIGA 190
           +  S     VI+GVLDTG+WPE  SFSD GLGPIP++WKGECE+G +F +SSCNRKLIGA
Sbjct: 129 WSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGA 188

Query: 191 RYFSKGYEAAFGPIDE--SQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAGTARGM 250
           R F +GY        +  ++ES+SPRD +GHG+HT++TAAGS V  A+L+ +A GTA GM
Sbjct: 189 RAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGM 248

Query: 251 AAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLG--GNTADFYRDNVAIGAF 310
           A++AR+A YK+CW GGC+ SDILAAMD++V DG +++S+S+G  G+  +++ D++AIGAF
Sbjct: 249 ASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAF 308

Query: 311 SAAAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGESLY 370
            A   G+ VSCSAGN GP+  T +N+APWI TVGA T+DR+F A    G+GK  TG SLY
Sbjct: 309 GATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLY 368

Query: 371 SGKPLPNSLLPIVYAASASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQKGLVVKE 430
           +G+ LP+S L +VY    S      LC    LN + V GKIV+CDRGGN+RV+KG  VK 
Sbjct: 369 AGESLPDSQLSLVY----SGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKL 428

Query: 431 AGGLGMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYISSDSNPTATISTGTTRLG 490
           AGG GMILANT   GEE  AD+HL+P   VG K+GD I+ YI +  +PTA IS   T +G
Sbjct: 429 AGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIG 488

Query: 491 VQ-PSPVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFNII 550
              PSP VAAFSSRGPN +TP ILKPD+IAPGVNILAGWTG  GPT LD D R V FNII
Sbjct: 489 PSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNII 548

Query: 551 SGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGSPSTPFD 610
           SGTSMSCPH+SGLAALL+ AHPDWSPAAI+SAL+TTAY    +GE I+D++ G  S  F 
Sbjct: 549 SGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFI 608

Query: 611 IGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDF---TCNGNKNYKL 670
            GAGHV+P  AL+PGLVYD    +Y+AFLCA+ Y    I V  +       C  +K    
Sbjct: 609 HGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTA 668

Query: 671 EDLNYPSFAVPLETPSTKGGENVAPTTVKYTRTLTNKGA--PSTYKVSVTAKSSSVKILV 730
            DLNYPSF+V   +     GE      VKY R + N G+   + Y+V V +  ++V+I V
Sbjct: 669 GDLNYPSFSVVFAST----GE-----VVKYKRVVKNVGSNVDAVYEVGVKS-PANVEIDV 728

Query: 731 EPESLSFTEANEQKSYTVTFTASPMPSG-----SQSFARLEWSDGKHIVGSPIAFTW 770
            P  L+F++      Y VTF +  +  G        F  +EW+DG+H+V SP+A  W
Sbjct: 729 SPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of PI0004912 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 700.3 bits (1806), Expect = 1.7e-201
Identity = 383/778 (49.23%), Postives = 512/778 (65.81%), Query Frame = 0

Query: 9   WFLLFLISFSSCLFTEAQKSNQQLKKKTYIIHMDKANMPQAFDDHFQWYDSSLKSVSDS- 68
           ++  FL++ SS        S       TYI+H+D    P  F  HF WY SSL S++ S 
Sbjct: 5   FYFFFLLTLSS-----PSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSP 64

Query: 69  AQMLYSYNTVIHGFSTRLTVEEAKLMEKQEGILAVTPEMKYELHTTRTPEFLGLGKS--V 128
             ++++Y+TV HGFS RLT ++A  +     +++V PE    LHTTR+PEFLGL  +   
Sbjct: 65  PSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKA 124

Query: 129 SFFPASVKVSGVIIGVLDTGVWPELESFSDDGLGPIPASWKGECEVGKNFTSSSCNRKLI 188
                S   S ++IGV+DTGVWPE  SF D GLGP+P  WKG+C   ++F  S+CNRKL+
Sbjct: 125 GLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLV 184

Query: 189 GARYFSKGYEAAFGPIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAGTARGM 248
           GAR+F  GYEA  G ++E+ E +SPRD DGHG+HT++ +AG  V  A+  G+A G A GM
Sbjct: 185 GARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGM 244

Query: 249 AAEARVATYKVCWLGGCFSSDILAAMDKSVEDGCNILSVSLGGNTADFYRDNVAIGAFSA 308
           A +AR+A YKVCW  GC+ SDILAA D +V DG +++S+S+GG    +Y D +AIGAF A
Sbjct: 245 APKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGA 304

Query: 309 AAQGVFVSCSAGNGGPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGESLYSG 368
             +G+FVS SAGNGGP + T++NVAPW+TTVGAGT+DRDFPA V LGNGK I+G S+Y G
Sbjct: 305 IDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGG 364

Query: 369 KPL-PNSLLPIVYAAS--ASNSSSGSLCLSSTLNPAKVVGKIVVCDRGGNSRVQKGLVVK 428
             L P  + P+VY  S    +  S SLCL  +L+P  V GKIV+CDRG NSR  KG +V+
Sbjct: 365 PGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVR 424

Query: 429 EAGGLGMILANTEAYGEEQLADAHLIPTAAVGQKSGDAIKSYIS------SDSNPTATIS 488
           + GGLGMI+AN    GE  +AD H++P  +VG   GD I+ YIS      S  +PTATI 
Sbjct: 425 KNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIV 484

Query: 489 TGTTRLGVQPSPVVAAFSSRGPNLITPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRH 548
              TRLG++P+PVVA+FS+RGPN  TP+ILKPD+IAPG+NILA W    GP+G+ SD R 
Sbjct: 485 FKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRR 544

Query: 549 VAFNIISGTSMSCPHISGLAALLKAAHPDWSPAAIRSALMTTAYSTYKNGETIQDISNGS 608
             FNI+SGTSM+CPH+SGLAALLKAAHPDWSPAAIRSAL+TTAY+   +GE + D S G+
Sbjct: 545 TEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGN 604

Query: 609 PSTPFDIGAGHVNPTAALDPGLVYDTTTDDYLAFLCALNYSSLQIKVISKKDFTCNGNKN 668
            S+  D G+GHV+PT A+DPGLVYD T+ DY+ FLC  NY+   I  I+++   C+G + 
Sbjct: 605 TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARR 664

Query: 669 Y-KLEDLNYPSFAVPLETPSTKGGENVAPTTVKYTRTLTNKG-APSTYKVSVTAKSSSVK 728
              + +LNYPSF+V  +    + GE+   T   + RT+TN G + S Y++ +     +  
Sbjct: 665 AGHVGNLNYPSFSVVFQ----QYGESKMST--HFIRTVTNVGDSDSVYEIKIRPPRGTT- 724

Query: 729 ILVEPESLSFTEANEQKSYTVTF--TASPMPSGSQSF--ARLEWSDGKHIVGSPIAFT 769
           + VEPE LSF    ++ S+ V    T   +  G+ +     + WSDGK  V SP+  T
Sbjct: 725 VTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770