PI0004871 (gene) Melon (PI 482460) v1

BLAST of PI0004871 vs. ExPASy Swiss-Prot
Match: O60826 (Coiled-coil domain-containing protein 22 OS=Homo sapiens OX=9606 GN=CCDC22 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.3e-27
Identity = 156/620 (25.16%), Postives = 269/620 (43.39%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPPDLSSIRNLSPPIFFSICAQVLNRIHPQTKIPFSTSLPDS 60
           MEE+  IL++SL+++G ++PPD+ ++R  +  +      + L  I+P      S  LP +
Sbjct: 1   MEEADRILIHSLRQAGTAVPPDVQTLRAFTTELVVEAVVRCLRVINPAVGSGLSPLLPLA 60

Query: 61  VADQFKVCSEISSAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLMERLP-EASD------ 120
           ++ +F++   ++ A  DLG+  ++ +  FLYPSE DL  L+ FL ERLP +AS+      
Sbjct: 61  MSARFRLAMSLAQACMDLGYPLELGYQNFLYPSEPDLRDLLLFLAERLPTDASEDADQPA 120

Query: 121 GKSKVLEDGDGRKER----------KVDTSRCYYVENQSNKIDTNDS------VSTHQKV 180
           G S +L    G + R           + T +  +++  + +   + S      +S+  + 
Sbjct: 121 GDSAILLRAIGSQIRDQLALPWVPPHLRTPKLQHLQGSALQKPFHASRLVVPELSSRGEP 180

Query: 181 ENKLADLNIIAEETKSPDSI-------------------VDRFSDFCLVRKSSGEAAMVD 240
               A   ++   T+ P  +                    DR  D   V ++S      D
Sbjct: 181 REFQASPLLLPVPTQVPQPVGRVASLLEHHALQLCQQTGRDRPGDEDWVHRTSRLPPQED 240

Query: 241 NLVNAPKDQSEVSGN--ESVGLQG---------------NNGHEAG-------TYIQK-T 300
                 + Q +++ +  +S GL G                 G + G       T+ +K T
Sbjct: 241 TRAQRQRLQKQLTEHLRQSWGLLGAPIQARDLGELLQAWGAGAKTGAPKGSRFTHSEKFT 300

Query: 301 FEDQEIRPLEEVS------------TNASELKHFQVELEMLKAVTRIVFD---DNHSIEF 360
           F  +      +VS            T A++ +  +   E L+ V R + +   D  ++  
Sbjct: 301 FHLEPQAQATQVSDVPATSRRPEQVTWAAQEQELESLREQLEGVNRSIEEVEADMKTLGV 360

Query: 361 HLQQLEEQINSRKLNLLEMKSQWDAER---ELLENKRRSLQESLCASNPKAQ-------- 420
              Q E +    KL+  E +     +    ELL +   +L +        AQ        
Sbjct: 361 SFVQAESECRHSKLSTAEREQALRLKSRAVELLPDGTANLAKLQLVVENSAQRVIHLAGQ 420

Query: 421 ---------------EKLQKLREFESEKELIEL------------EIRRREEENSNIAID 480
                           KLQ  RE ES + L E+            E RR+EE    +  +
Sbjct: 421 WEKHRVPLLAEYRHLRKLQDCRELESSRRLAEIQELHQSVRAAAEEARRKEEVYKQLMSE 480

Query: 481 LKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 499
           L+  P+ +SR +Y  R+ EI  N RKQ  +I +IL +T+ELQ E N++  +L RT+AV D
Sbjct: 481 LETLPRDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTFAVTD 540

BLAST of PI0004871 vs. ExPASy Swiss-Prot
Match: Q28G12 (Coiled-coil domain-containing protein 22 OS=Xenopus tropicalis OX=8364 GN=ccdc22 PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 4.8e-27
Identity = 141/625 (22.56%), Postives = 265/625 (42.40%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPPDLSSIRNLSPPIFFSICAQVLNRIHPQTKIPFSTSLPDS 60
           MEE   IL++SL+  G  +P D+ SIR  +  +      + L  I+P      S  LP  
Sbjct: 1   MEEVDRILIHSLRSCGTEVPEDVQSIRQFNTELIVEAVVRCLRVINPSLGATLSHVLPPG 60

Query: 61  VADQFKVCSEISSAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLMERLPEASD------- 120
           ++ +F++ + ++ A +DLG+ G++ +  FLY SE D+  L+ FL E+LP  S        
Sbjct: 61  MSARFRIGTSLAQACQDLGYPGEVGYQTFLYSSEPDIRALLIFLAEKLPRDSPEDAHQPA 120

Query: 121 GKSKVLEDGDGRK-------------------ERKVDTSRCYYVENQSNKIDTN---DSV 180
           GKS +L+                          R  ++ R +    Q   + T+    S+
Sbjct: 121 GKSALLQREIAATIKRQLSLPWLPSSCRICALRRSQNSCRLHRFHAQPLSLATDPTLKSI 180

Query: 181 STHQK---------VENKLADL-NIIAEETKSPDSIVDRFSDFCLVRKSSGEAA------ 240
              +K         V ++L  L ++ A   +   S +    ++    KS G A+      
Sbjct: 181 PDERKEYWQCYLPSVTSQLPHLPSVAASLLERNTSELSAKQEWDAEWKSQGLASRLSPED 240

Query: 241 -----------------------MVDNLVNAPKDQSEVSGNESVGLQGNNGHEAG----- 300
                                  + +N + +   Q      ++  L G +  + G     
Sbjct: 241 YRSRKRQRLQKRIQEQLRQCAQLLAENHLPSSSSQDLTDMLKAFNLDGGSDQKKGSRFTR 300

Query: 301 ----TYIQ--KTFEDQ-----EIRP---LEEVSTNASELKHFQVELEMLKAVTRIVFDDN 360
               TY Q   T ++Q     EI P    ++      EL   Q +++ ++   R + + N
Sbjct: 301 TQRFTYQQDPHTLKEQMQRAAEILPKKDAQDTDAEQQELSSLQEQIDSIEQEIRGLSESN 360

Query: 361 HSIEFHLQQLEEQINSRKL------------------------NLLEMKS---------- 420
             ++  + Q+E ++N  +                         NL+++++          
Sbjct: 361 KRLQLTVSQVEGEVNEMRQSCEEKEKIVRVKKRAVELLPDADNNLVKLQALVDASSHRMA 420

Query: 421 ----QWDAERELLENKRRSLQESLCASNPKAQEKLQKLREFESEKELIELEIRRREEENS 480
               QW++ +  L  + R L+        ++   ++  ++   +      E +R+EE   
Sbjct: 421 NLVGQWESHQVRLSEEYRELKRVQQEQEDESSRWMKDAKDLYEKIRGAADEAKRKEELYK 480

Query: 481 NIAIDLKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRT 499
            +  + +  PK++SR +Y  R+ EI  N +KQ  +I +IL +T+ELQ E NN+  ++ RT
Sbjct: 481 QLLSEYESLPKEVSRAAYTQRILEIVSNIKKQKEEITKILSDTKELQKEINNLTGKVDRT 540

BLAST of PI0004871 vs. ExPASy Swiss-Prot
Match: Q6PA15 (Coiled-coil domain-containing protein 22 OS=Xenopus laevis OX=8355 GN=ccdc22 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.4e-26
Identity = 146/628 (23.25%), Postives = 265/628 (42.20%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPPDLSSIRNLSPPIFFSICAQVLNRIHPQTKIPFSTSLPDS 60
           MEE   IL++SL+  G  +P D+ SIR  +  +      + L  I+P      S  LP  
Sbjct: 1   MEEVDRILIHSLRSCGTEVPEDIQSIRQFNTELIVEAVVRCLRVINPSLGATLSHVLPPG 60

Query: 61  VADQFKVCSEISSAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLMERLPEASD------- 120
           ++ QF++ + ++ A +DLG+  ++ +  FLY SE D+  L+ FL E+LP  S        
Sbjct: 61  MSAQFRIGTSLAQACQDLGYSSEVGYQTFLYSSEPDIRALLIFLAEKLPRDSSEDAHQPA 120

Query: 121 GKSKVLED-------------------GDGRKERKVDTSRCYYVENQSNKIDTN---DSV 180
           GKS +L+                          R   + R +   +Q   + ++    S+
Sbjct: 121 GKSALLQREIVATIKQQLSLPWLPLSCRIAALRRSQSSCRLHRFHSQPLSLASDPALKSI 180

Query: 181 STHQK---------VENKLADL-NIIAEETKSPDSIVDRFSDFCLVRKSSGEAA------ 240
              +K         V ++L  L ++ A   +   S +    ++    KS G A+      
Sbjct: 181 PNERKEYWQRYLPSVTSQLPHLPSVAASLLERNTSKLSAVQEWEAEWKSQGLASRLSPED 240

Query: 241 -----------------------MVDNLVNAPKDQSEVSGNESVGLQGNNGHEAG----- 300
                                  + +N + +   Q       +  L G +  + G     
Sbjct: 241 YRSKKQQRLQKRIQEQLRQCAQLLAENHLPSSSSQDLTDMLRAFNLDGGSDQKKGSRFTR 300

Query: 301 ----TYIQ--KTFEDQ-----EIRP---LEEVSTNASELKHFQVELEMLKAVTRIVFDDN 360
               TY Q   T ++Q     EI P    ++      E+   Q +++ ++   R + + N
Sbjct: 301 TQRFTYQQDPHTLKEQMQRAAEILPKKDAQDADAEQQEISSLQEQIDSIEQEIRGLSESN 360

Query: 361 HSIEFHLQQLEEQIN------SRKLNLLEMKSQWDAERELLENKRRSLQESLCASNPKAQ 420
             ++  + Q+E ++N        K N++ +K +     ELL +   +L +     +  AQ
Sbjct: 361 KLLQLTIGQVEGEVNDMHQSCEEKANVVRVKKR---AVELLPDADNNLDKLQTLVDASAQ 420

Query: 421 EKLQKLREFESEK-----ELIEL------------------------------EIRRREE 480
                + ++ES +     E +EL                              E +R+EE
Sbjct: 421 RMANLIGQWESHQARLSDEYMELNRVQQEQEDESSRWMKDAKDLYEKIQGSADEAKRKEE 480

Query: 481 ENSNIAIDLKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERL 499
               +  + +  PK++SR +Y  R+ EI  N +KQ  +I +IL +T+ELQ E NN+  ++
Sbjct: 481 LYKQLLSEYESLPKEVSRAAYTQRILEIVSNIKKQKEEITKILSDTKELQKEINNLTGKV 540

BLAST of PI0004871 vs. ExPASy Swiss-Prot
Match: Q9JIG7 (Coiled-coil domain-containing protein 22 OS=Mus musculus OX=10090 GN=Ccdc22 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 2.4e-26
Identity = 148/620 (23.87%), Postives = 267/620 (43.06%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPPDLSSIRNLSPPIFFSICAQVLNRIHPQTKIPFSTSLPDS 60
           MEE+  IL++SL+++G ++PP++ ++R  +  +      + L  I+P      S  LP +
Sbjct: 1   MEEADRILIHSLRQAGTAVPPEVQTLRAFTTELVVEAVVRCLRVINPDVGSGLSHLLPPA 60

Query: 61  VADQFKVCSEISSAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLMERLP-EASD------ 120
           ++ +F++   ++ A  DLG+  ++ +  FLYPSE DL  L+ FL ERLP +AS+      
Sbjct: 61  MSARFRLAMSLAQACMDLGYPLELGYQNFLYPSEPDLRDLLLFLAERLPSDASEDADQPA 120

Query: 121 GKSKVLEDGDGRKER---------------KVDTSRCYYVEN--QSNKIDTNDSVSTHQK 180
           G S +     G + R               KV   +   ++    S+++   +  S+ + 
Sbjct: 121 GDSAIFLRAIGSQIRDQLALPWVPPLLRTPKVQRLQGSALQQPFHSSRLVLPELNSSGEL 180

Query: 181 VENKLADLNIIAE------ETKSPDSIVDRFSDFC--LVRKSSGEAAMVDNLVNAPKDQS 240
            E + + L + A       + ++   +    S  C  + R   G+   V      P  + 
Sbjct: 181 WEFQASPLLLPAPTQVPQLQGRAASLLEHHASQLCQHVNRDCPGDEDRVRWASRVPSQED 240

Query: 241 EVSGNESV-------------------------------GLQGNNGHEAG---TYIQK-T 300
             +  + +                               G +   G   G   T+ +K T
Sbjct: 241 SRAPQQRLHKQLIEHLRQSWGPLGAPTQVRDLGEMLQTWGARAMTGVPKGSRFTHSEKFT 300

Query: 301 F----------------------------EDQEIRPLEE----VSTNASELKH------- 360
           F                            ++QE+  L E    V+ N  E++        
Sbjct: 301 FHLEPQVQAAQVADVPATSQRLEQDTRAAQEQELESLREQLASVNHNIEEVEADMKTLGI 360

Query: 361 --FQVELEMLKAVTRIVFDD------NHSIEF------HLQQLEEQINSRKLNLLEMKSQ 420
              QVE E  ++   +   +      + ++E       +L +L+  + S    L+ + SQ
Sbjct: 361 NLVQVETECRQSELSVAEQEQALRLKSRTVELLPDGAANLAKLQLVVESSAQRLIHLASQ 420

Query: 421 WDAERELLENKRRSLQESLCASNPKAQEKLQKLREFESEKELIELEIRRREEENSNIAID 480
           W+  R  L  + R L+        ++  +L +++E          E RR+EE    +  +
Sbjct: 421 WEKHRVPLLAEYRHLRRLQDCRELESSRRLAEIQELHHSVRAAAEEARRKEEVYKQLVSE 480

Query: 481 LKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 499
           L+  PK +SR +Y  R+ EI  N RKQ  +I +IL +T+ELQ E N++  +L RT+AV D
Sbjct: 481 LETLPKDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSLSGKLDRTFAVTD 540

BLAST of PI0004871 vs. ExPASy Swiss-Prot
Match: P86182 (Coiled-coil domain-containing protein 22 OS=Rattus norvegicus OX=10116 GN=Ccdc22 PE=1 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 3.1e-26
Identity = 157/632 (24.84%), Postives = 268/632 (42.41%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPPDLSSIRNLSPPIFFSICAQVLNRIHPQTKIPFSTSLPDS 60
           MEE+  IL++SL+++G ++PP++ ++R  +  +      + L  I+P      S  LP +
Sbjct: 1   MEEADRILIHSLRQAGTAVPPEVQTLRAFTTELVVEAVVRCLRVINPDVGSGLSHLLPPA 60

Query: 61  VADQFKVCSEISSAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLMERLP-EASD------ 120
           ++ +F++   ++ A  DLG+  ++ +  FLYPSE DL  L+ FL ERLP +AS+      
Sbjct: 61  MSARFRLAMSLAQACMDLGYPLELGYQNFLYPSEPDLRDLLLFLAERLPTDASEDADQPA 120

Query: 121 GKSKVLEDGDGRKERKVDTSRCYYVENQSNKIDTNDSVSTHQKVENK---LADLNIIAEE 180
           G S +     G + R            ++ K+      +  Q   +    L +LN   E 
Sbjct: 121 GDSAIFLRAIGSQIRDQLALPWVPPLLRTPKVQRLQGSALQQAFHSSRLVLPELNCRGEP 180

Query: 181 TK---------SPDSIV-----------------------DRFSDFCLVRKSSG------ 240
            +         +P  +                        D   D   VR +S       
Sbjct: 181 REFQASPLLLPAPSQVPQLLGRAASLLEHHAIQLCQHVNRDCPGDEDRVRWASRLPSQED 240

Query: 241 --------EAAMVDNL------VNAPKDQSEVSGN-ESVGLQGNNGHEAG---TYIQK-T 300
                      ++++L      + AP    ++    ++ G +   G   G   T+ +K T
Sbjct: 241 PRAPQQRLHKQLIEHLRQSWGPLGAPTQVRDLGEMLQAWGAKAMTGVPKGSRFTHSEKFT 300

Query: 301 F----------------------------EDQEIRPL-----------EEVSTNASEL-- 360
           F                            +++E+  L           EEV  N   L  
Sbjct: 301 FHLEPQVQAAQVADIPAASQRPEQDTRAAQEEELESLREQLASVNHNIEEVEANMKSLGM 360

Query: 361 KHFQVELEMLKAVTRIVFDD------NHSIEF------HLQQLEEQINSRKLNLLEMKSQ 420
              QVE E  ++   +   +      + ++E       +L +L+  + S    L+ + SQ
Sbjct: 361 NLVQVETECRQSELSVAEQEQALRLKSRTVELLPDGAANLTKLQLVVESSAQRLIHLASQ 420

Query: 421 WDAERELLENKRRSLQESLCASNPKAQEKLQKLREFESEKELIEL------------EIR 480
           W+  R  L  + R L+            KLQ  RE ES + L+E+            E R
Sbjct: 421 WEKHRVPLLAEYRHLR------------KLQDCRELESSRRLVEIQELHQSVRAAAEEAR 480

Query: 481 RREEENSNIAIDLKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNI 499
           R+EE    +  +L+  PK +SR +Y  R+ EI  N RKQ  +I +IL +T+ELQ E N++
Sbjct: 481 RKEEVYKQLVSELETLPKDVSRLAYTQRILEIVGNIRKQKEEITKILSDTKELQKEINSL 540

BLAST of PI0004871 vs. ExPASy TrEMBL
Match: A0A0A0L4U9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G047850 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.4e-255
Identity = 478/511 (93.54%), Postives = 489/511 (95.69%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPPDLSSIRNLSPPIFFSICAQVLNRIHPQTKIPFSTSLPDS 60
           MEESQEILLNSLQKSGVSIP DLSSIRNLSPPIFFSICAQVLNRIHPQTK PFSTSLPDS
Sbjct: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60

Query: 61  VADQFKVCSEISSAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLMERLPEASDGKSKVLE 120
           VADQFKV SEI+SAIKDLGFIGDISFHKFLYPSEEDLYKLIRFL+ERLPEASDGK KVLE
Sbjct: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKFKVLE 120

Query: 121 DGDGRKERKVDTSRCYYVENQSNKIDTNDSVSTHQKVENKLADLNIIAEETKSPDSIVDR 180
           DGDGRKE K DTS+CYYVENQSNKIDT+DSVSTHQKVENKLADLNI+AEETKSPDSIVDR
Sbjct: 121 DGDGRKETKADTSKCYYVENQSNKIDTDDSVSTHQKVENKLADLNIVAEETKSPDSIVDR 180

Query: 181 FSDFCLVRKSSGEAAMVDNLVNAPKDQSEVSGNESVGLQGNNGHEAGTYIQKTFEDQEIR 240
           FSDF L RKSSGEAAMVDNLVNA KDQSEVSGNESV LQGNN HE GTYIQKTFEDQEI 
Sbjct: 181 FSDFHLDRKSSGEAAMVDNLVNASKDQSEVSGNESVELQGNNRHEVGTYIQKTFEDQEIG 240

Query: 241 PLEEVSTNASELKHFQVELEMLKAVTRIVFDDNHSIEFHLQQLEEQINSRKLNLLEMKSQ 300
           PLEEV+TNASELKHFQVELE LKAVT IVFDDNHSIEFHL+QLEEQINSRKL+LLEMKS+
Sbjct: 241 PLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLEMKSR 300

Query: 301 WDAERELLENKRRSLQESLCASNPKAQEKLQKLREFESEKELIELEIRRREEENSNIAID 360
           WDAERELLE KRRSLQESLCASNPKAQEKLQKLREFESEK+LIELEIRRREEENSNIAID
Sbjct: 301 WDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSNIAID 360

Query: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420
           LKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD
Sbjct: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420

Query: 421 ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480
           ELVLREAKKD  GKQAHKLLT+IHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN
Sbjct: 421 ELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480

Query: 481 FNKLQADLDAIRRENSRLEQHLCHIKPIDKV 512
           FNKLQADLDAIRREN  LEQHLCH K IDKV
Sbjct: 481 FNKLQADLDAIRRENGHLEQHLCHKKTIDKV 511

BLAST of PI0004871 vs. ExPASy TrEMBL
Match: A0A1S3BKI6 (coiled-coil domain-containing protein 22 OS=Cucumis melo OX=3656 GN=LOC103491028 PE=3 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.3e-253
Identity = 468/511 (91.59%), Postives = 487/511 (95.30%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPPDLSSIRNLSPPIFFSICAQVLNRIHPQTKIPFSTSLPDS 60
           MEESQEILLNSLQKS VSIPPDLSSIRNLSPPIFFSICAQVLN+IHPQTK PFSTSLPDS
Sbjct: 1   MEESQEILLNSLQKSSVSIPPDLSSIRNLSPPIFFSICAQVLNQIHPQTKTPFSTSLPDS 60

Query: 61  VADQFKVCSEISSAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLMERLPEASDGKSKVLE 120
           VADQFKVCS I+SAIKDLGFIGDISFHKFLYPSEEDLYKLIRFL+ERLPEASDGKSKVLE
Sbjct: 61  VADQFKVCSGIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120

Query: 121 DGDGRKERKVDTSRCYYVENQSNKIDTNDSVSTHQKVENKLADLNIIAEETKSPDSIVDR 180
           DGDGRKE K +TSRCYYVENQ NK DT+DSVSTHQKV++KLAD NIIAEET+SPDSI+DR
Sbjct: 121 DGDGRKETKANTSRCYYVENQPNKTDTDDSVSTHQKVQDKLADPNIIAEETRSPDSIIDR 180

Query: 181 FSDFCLVRKSSGEAAMVDNLVNAPKDQSEVSGNESVGLQGNNGHEAGTYIQKTFEDQEIR 240
           FSDFCL+RKS GEA MVDNLVNAPKD SEV GNESVGLQGNN HE GTYIQKTFEDQ I 
Sbjct: 181 FSDFCLIRKSLGEAPMVDNLVNAPKDHSEVCGNESVGLQGNNRHEVGTYIQKTFEDQAIG 240

Query: 241 PLEEVSTNASELKHFQVELEMLKAVTRIVFDDNHSIEFHLQQLEEQINSRKLNLLEMKSQ 300
           PLEEV+TNASELKHFQVELE L+AVTRIVFDDNHSIEFHLQQLEEQINS+KL+LLEMKSQ
Sbjct: 241 PLEEVTTNASELKHFQVELERLEAVTRIVFDDNHSIEFHLQQLEEQINSKKLDLLEMKSQ 300

Query: 301 WDAERELLENKRRSLQESLCASNPKAQEKLQKLREFESEKELIELEIRRREEENSNIAID 360
           WDAERELLE KRRSLQESLCA NP AQEKL+KLREFESEK+LIELEIRRREEENSNI ID
Sbjct: 301 WDAERELLEIKRRSLQESLCARNPMAQEKLKKLREFESEKKLIELEIRRREEENSNITID 360

Query: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420
           LKKQPKQ SRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD
Sbjct: 361 LKKQPKQSSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420

Query: 421 ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480
           ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSL+
Sbjct: 421 ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLD 480

Query: 481 FNKLQADLDAIRRENSRLEQHLCHIKPIDKV 512
           FNKLQADLDAIRRENS LEQHLCH KP+D+V
Sbjct: 481 FNKLQADLDAIRRENSHLEQHLCHKKPVDEV 511

BLAST of PI0004871 vs. ExPASy TrEMBL
Match: A0A5A7VC01 (Coiled-coil domain-containing protein 22 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00180 PE=3 SV=1)

HSP 1 Score: 884.0 bits (2283), Expect = 2.9e-253
Identity = 469/511 (91.78%), Postives = 488/511 (95.50%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPPDLSSIRNLSPPIFFSICAQVLNRIHPQTKIPFSTSLPDS 60
           MEESQEILLNSLQKS VSIPPDLSSIRNLSPPIFFSICAQVLN+IHPQTK PFSTSLPDS
Sbjct: 1   MEESQEILLNSLQKSSVSIPPDLSSIRNLSPPIFFSICAQVLNQIHPQTKTPFSTSLPDS 60

Query: 61  VADQFKVCSEISSAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLMERLPEASDGKSKVLE 120
           VADQFKVCSEI+SAIKDLGFIGDISFHKFLYPSEEDLYKLIRFL+ERLPEASDGKSKVLE
Sbjct: 61  VADQFKVCSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120

Query: 121 DGDGRKERKVDTSRCYYVENQSNKIDTNDSVSTHQKVENKLADLNIIAEETKSPDSIVDR 180
           DGDGRKE K +TSRCYYVENQ NK DT+DSVSTHQKV++KLAD NIIAEET+SPDSI+DR
Sbjct: 121 DGDGRKE-KANTSRCYYVENQPNKTDTDDSVSTHQKVQDKLADPNIIAEETRSPDSIIDR 180

Query: 181 FSDFCLVRKSSGEAAMVDNLVNAPKDQSEVSGNESVGLQGNNGHEAGTYIQKTFEDQEIR 240
           FSDFCL+RKS GEA MVDNLVNAPKD SEV GNESVGLQGNN HE GTYIQKTFEDQ I 
Sbjct: 181 FSDFCLIRKSLGEAPMVDNLVNAPKDHSEVCGNESVGLQGNNRHEVGTYIQKTFEDQAIG 240

Query: 241 PLEEVSTNASELKHFQVELEMLKAVTRIVFDDNHSIEFHLQQLEEQINSRKLNLLEMKSQ 300
           PLEEV+TNASELKHFQVELE L+AVTRIVFDDNHSIEFHLQQLEEQINS+KL+LLEMKSQ
Sbjct: 241 PLEEVTTNASELKHFQVELERLEAVTRIVFDDNHSIEFHLQQLEEQINSKKLDLLEMKSQ 300

Query: 301 WDAERELLENKRRSLQESLCASNPKAQEKLQKLREFESEKELIELEIRRREEENSNIAID 360
           WDAERELLE KRRSLQESLCA NP AQEKL+KLREFESEK+LIELEIRRREEENSNI ID
Sbjct: 301 WDAERELLEIKRRSLQESLCARNPMAQEKLKKLREFESEKKLIELEIRRREEENSNITID 360

Query: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420
           LKKQPKQ SRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD
Sbjct: 361 LKKQPKQSSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420

Query: 421 ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480
           ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSL+
Sbjct: 421 ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLD 480

Query: 481 FNKLQADLDAIRRENSRLEQHLCHIKPIDKV 512
           FNKLQADLDAIRRENS LEQHLCH KP+D+V
Sbjct: 481 FNKLQADLDAIRRENSHLEQHLCHKKPVDEV 510

BLAST of PI0004871 vs. ExPASy TrEMBL
Match: A0A6J1EW97 (coiled-coil domain-containing protein 22 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111438763 PE=3 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 2.0e-225
Identity = 426/517 (82.40%), Postives = 464/517 (89.75%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPPDLSSIRNLSPPIFFSICAQVLNRIHPQTKIPFSTSLPDS 60
           MEESQEILLNSLQKSGVS+PPD+SSIRNL+PP+ FSICAQVLNRIH QT+IP++TSLPDS
Sbjct: 1   MEESQEILLNSLQKSGVSVPPDVSSIRNLTPPLLFSICAQVLNRIHGQTQIPYATSLPDS 60

Query: 61  VADQFKVCSEISSAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLMERLPEASDGKSKVLE 120
           VADQFK+C+EI+SAIKDLG+IGD+SFHKFLYPSEEDLYKLIRFL+ERLPE SDGK+KVLE
Sbjct: 61  VADQFKICTEIASAIKDLGYIGDMSFHKFLYPSEEDLYKLIRFLVERLPEVSDGKTKVLE 120

Query: 121 DGDGRKERKVDTSRCYYVENQSNKIDTNDSVSTHQKVENKLADLNIIAEETKSPDSIVDR 180
           D DGRKE K DT +C+YV  QS+ IDT+DSV THQKVENKL+DLNIIAEETKS DSIV R
Sbjct: 121 DVDGRKETKTDTLKCHYVSGQSDDIDTDDSVFTHQKVENKLSDLNIIAEETKSSDSIVGR 180

Query: 181 FSDFCLVRKSSGEAAMVDNLVNAPKDQSEVSGNESVGLQGNNG------HEAGTYIQKTF 240
           FSD       SGEAAMVDNLVNAPKDQSEVSGNESV +QGNNG      HE  +YIQK  
Sbjct: 181 FSD-------SGEAAMVDNLVNAPKDQSEVSGNESVAVQGNNGQVSISRHEVSSYIQKND 240

Query: 241 EDQEIRPLEEVSTNASELKHFQVELEMLKAVTRIVFDDNHSIEFHLQQLEEQINSRKLNL 300
           EDQEIRPLEEVST ASEL+H  VELE LKA T IVFDDN  IE HLQ+LEEQI+SRKLNL
Sbjct: 241 EDQEIRPLEEVSTKASELEHLHVELERLKAATGIVFDDNRLIEVHLQELEEQISSRKLNL 300

Query: 301 LEMKSQWDAERELLENKRRSLQESLCASNPKAQEKLQKLREFESEKELIELEIRRREEEN 360
           L  KS W AE+ELLENKRR+LQESLCASNPKAQEKLQKLREFE EK+LIE EI RREEEN
Sbjct: 301 LAAKSGWAAEKELLENKRRNLQESLCASNPKAQEKLQKLREFELEKQLIESEISRREEEN 360

Query: 361 SNIAIDLKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHR 420
            N++ +LKKQPKQ SRRSYI+RVKEITKNSRKQD DIDRILKETRELQLESNNIQERLHR
Sbjct: 361 YNLSTELKKQPKQASRRSYIDRVKEITKNSRKQDTDIDRILKETRELQLESNNIQERLHR 420

Query: 421 TYAVVDELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAAS 480
           TYA+VDELVLREAKK+GIGK+AHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAAS
Sbjct: 421 TYAIVDELVLREAKKEGIGKKAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAAS 480

Query: 481 ASRSLNFNKLQADLDAIRRENSRLEQHLCHIKPIDKV 512
           ASRSL+FNKLQADLDAIRRENS LEQ+LCH KPID++
Sbjct: 481 ASRSLDFNKLQADLDAIRRENSCLEQNLCHKKPIDQM 510

BLAST of PI0004871 vs. ExPASy TrEMBL
Match: A0A6J1L5Y0 (coiled-coil domain-containing protein 22 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500292 PE=3 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 2.9e-224
Identity = 425/517 (82.21%), Postives = 462/517 (89.36%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPPDLSSIRNLSPPIFFSICAQVLNRIHPQTKIPFSTSLPDS 60
           MEESQEILLNSLQKSGVS+PPD+SSIR L+PP+ FSICAQVLNRIH QT+IP++TSLPDS
Sbjct: 1   MEESQEILLNSLQKSGVSVPPDVSSIRKLTPPLLFSICAQVLNRIHGQTQIPYATSLPDS 60

Query: 61  VADQFKVCSEISSAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLMERLPEASDGKSKVLE 120
           VADQFK+C+EI+SAIKDLG+IGD+SFHKFLYPSEEDLYKLIRFL+ERLPE SDGKSKVLE
Sbjct: 61  VADQFKICTEIASAIKDLGYIGDMSFHKFLYPSEEDLYKLIRFLVERLPEVSDGKSKVLE 120

Query: 121 DGDGRKERKVDTSRCYYVENQSNKIDTNDSVSTHQKVENKLADLNIIAEETKSPDSIVDR 180
           DGDGRKE K DT +C+YV  QS+ IDT DSV THQKVENKL+DLNIIAEETKS DSIV R
Sbjct: 121 DGDGRKETKADTLKCHYVSGQSDAIDTADSVFTHQKVENKLSDLNIIAEETKSSDSIVGR 180

Query: 181 FSDFCLVRKSSGEAAMVDNLVNAPKDQSEVSGNESVGLQGNNG------HEAGTYIQKTF 240
            SD       SGE AMVDNLVNAPK QSEVSGNESV +QGNNG      HE  +YIQK  
Sbjct: 181 LSD-------SGETAMVDNLVNAPKYQSEVSGNESVAVQGNNGQVSISRHEVSSYIQKND 240

Query: 241 EDQEIRPLEEVSTNASELKHFQVELEMLKAVTRIVFDDNHSIEFHLQQLEEQINSRKLNL 300
           EDQEIRPLEEVST ASEL+H  VELE LKA T IVFDDN SIE HLQ+LEEQI+SRKLNL
Sbjct: 241 EDQEIRPLEEVSTKASELEHLLVELERLKAATGIVFDDNRSIEVHLQELEEQISSRKLNL 300

Query: 301 LEMKSQWDAERELLENKRRSLQESLCASNPKAQEKLQKLREFESEKELIELEIRRREEEN 360
           L  KS WDAE+ELLENKRR+LQESLCASNPKA EKLQKLREFE EK+LIE EIRRREE N
Sbjct: 301 LAAKSGWDAEKELLENKRRNLQESLCASNPKAPEKLQKLREFELEKQLIESEIRRREEGN 360

Query: 361 SNIAIDLKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHR 420
            N++ +LKKQPKQ SRRSYI+RVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHR
Sbjct: 361 YNLSTELKKQPKQASRRSYIDRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHR 420

Query: 421 TYAVVDELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAAS 480
           TYA+VDELVLREAKK+GIGK+AHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAAS
Sbjct: 421 TYAIVDELVLREAKKEGIGKKAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAAS 480

Query: 481 ASRSLNFNKLQADLDAIRRENSRLEQHLCHIKPIDKV 512
           ASRSL+FNKLQADLDAIR+ENS LEQ+LCH KPID++
Sbjct: 481 ASRSLDFNKLQADLDAIRKENSCLEQNLCHKKPIDQM 510

BLAST of PI0004871 vs. NCBI nr
Match: XP_004148284.1 (coiled-coil domain-containing protein 22 isoform X1 [Cucumis sativus])

HSP 1 Score: 891.7 bits (2303), Expect = 2.9e-255
Identity = 478/511 (93.54%), Postives = 489/511 (95.69%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPPDLSSIRNLSPPIFFSICAQVLNRIHPQTKIPFSTSLPDS 60
           MEESQEILLNSLQKSGVSIP DLSSIRNLSPPIFFSICAQVLNRIHPQTK PFSTSLPDS
Sbjct: 1   MEESQEILLNSLQKSGVSIPTDLSSIRNLSPPIFFSICAQVLNRIHPQTKTPFSTSLPDS 60

Query: 61  VADQFKVCSEISSAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLMERLPEASDGKSKVLE 120
           VADQFKV SEI+SAIKDLGFIGDISFHKFLYPSEEDLYKLIRFL+ERLPEASDGK KVLE
Sbjct: 61  VADQFKVSSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKFKVLE 120

Query: 121 DGDGRKERKVDTSRCYYVENQSNKIDTNDSVSTHQKVENKLADLNIIAEETKSPDSIVDR 180
           DGDGRKE K DTS+CYYVENQSNKIDT+DSVSTHQKVENKLADLNI+AEETKSPDSIVDR
Sbjct: 121 DGDGRKETKADTSKCYYVENQSNKIDTDDSVSTHQKVENKLADLNIVAEETKSPDSIVDR 180

Query: 181 FSDFCLVRKSSGEAAMVDNLVNAPKDQSEVSGNESVGLQGNNGHEAGTYIQKTFEDQEIR 240
           FSDF L RKSSGEAAMVDNLVNA KDQSEVSGNESV LQGNN HE GTYIQKTFEDQEI 
Sbjct: 181 FSDFHLDRKSSGEAAMVDNLVNASKDQSEVSGNESVELQGNNRHEVGTYIQKTFEDQEIG 240

Query: 241 PLEEVSTNASELKHFQVELEMLKAVTRIVFDDNHSIEFHLQQLEEQINSRKLNLLEMKSQ 300
           PLEEV+TNASELKHFQVELE LKAVT IVFDDNHSIEFHL+QLEEQINSRKL+LLEMKS+
Sbjct: 241 PLEEVTTNASELKHFQVELERLKAVTSIVFDDNHSIEFHLRQLEEQINSRKLDLLEMKSR 300

Query: 301 WDAERELLENKRRSLQESLCASNPKAQEKLQKLREFESEKELIELEIRRREEENSNIAID 360
           WDAERELLE KRRSLQESLCASNPKAQEKLQKLREFESEK+LIELEIRRREEENSNIAID
Sbjct: 301 WDAERELLEIKRRSLQESLCASNPKAQEKLQKLREFESEKKLIELEIRRREEENSNIAID 360

Query: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420
           LKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD
Sbjct: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420

Query: 421 ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480
           ELVLREAKKD  GKQAHKLLT+IHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN
Sbjct: 421 ELVLREAKKDDTGKQAHKLLTNIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480

Query: 481 FNKLQADLDAIRRENSRLEQHLCHIKPIDKV 512
           FNKLQADLDAIRREN  LEQHLCH K IDKV
Sbjct: 481 FNKLQADLDAIRRENGHLEQHLCHKKTIDKV 511

BLAST of PI0004871 vs. NCBI nr
Match: XP_008449035.1 (PREDICTED: coiled-coil domain-containing protein 22 [Cucumis melo])

HSP 1 Score: 885.2 bits (2286), Expect = 2.7e-253
Identity = 468/511 (91.59%), Postives = 487/511 (95.30%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPPDLSSIRNLSPPIFFSICAQVLNRIHPQTKIPFSTSLPDS 60
           MEESQEILLNSLQKS VSIPPDLSSIRNLSPPIFFSICAQVLN+IHPQTK PFSTSLPDS
Sbjct: 1   MEESQEILLNSLQKSSVSIPPDLSSIRNLSPPIFFSICAQVLNQIHPQTKTPFSTSLPDS 60

Query: 61  VADQFKVCSEISSAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLMERLPEASDGKSKVLE 120
           VADQFKVCS I+SAIKDLGFIGDISFHKFLYPSEEDLYKLIRFL+ERLPEASDGKSKVLE
Sbjct: 61  VADQFKVCSGIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120

Query: 121 DGDGRKERKVDTSRCYYVENQSNKIDTNDSVSTHQKVENKLADLNIIAEETKSPDSIVDR 180
           DGDGRKE K +TSRCYYVENQ NK DT+DSVSTHQKV++KLAD NIIAEET+SPDSI+DR
Sbjct: 121 DGDGRKETKANTSRCYYVENQPNKTDTDDSVSTHQKVQDKLADPNIIAEETRSPDSIIDR 180

Query: 181 FSDFCLVRKSSGEAAMVDNLVNAPKDQSEVSGNESVGLQGNNGHEAGTYIQKTFEDQEIR 240
           FSDFCL+RKS GEA MVDNLVNAPKD SEV GNESVGLQGNN HE GTYIQKTFEDQ I 
Sbjct: 181 FSDFCLIRKSLGEAPMVDNLVNAPKDHSEVCGNESVGLQGNNRHEVGTYIQKTFEDQAIG 240

Query: 241 PLEEVSTNASELKHFQVELEMLKAVTRIVFDDNHSIEFHLQQLEEQINSRKLNLLEMKSQ 300
           PLEEV+TNASELKHFQVELE L+AVTRIVFDDNHSIEFHLQQLEEQINS+KL+LLEMKSQ
Sbjct: 241 PLEEVTTNASELKHFQVELERLEAVTRIVFDDNHSIEFHLQQLEEQINSKKLDLLEMKSQ 300

Query: 301 WDAERELLENKRRSLQESLCASNPKAQEKLQKLREFESEKELIELEIRRREEENSNIAID 360
           WDAERELLE KRRSLQESLCA NP AQEKL+KLREFESEK+LIELEIRRREEENSNI ID
Sbjct: 301 WDAERELLEIKRRSLQESLCARNPMAQEKLKKLREFESEKKLIELEIRRREEENSNITID 360

Query: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420
           LKKQPKQ SRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD
Sbjct: 361 LKKQPKQSSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420

Query: 421 ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480
           ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSL+
Sbjct: 421 ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLD 480

Query: 481 FNKLQADLDAIRRENSRLEQHLCHIKPIDKV 512
           FNKLQADLDAIRRENS LEQHLCH KP+D+V
Sbjct: 481 FNKLQADLDAIRRENSHLEQHLCHKKPVDEV 511

BLAST of PI0004871 vs. NCBI nr
Match: KAA0065733.1 (coiled-coil domain-containing protein 22 [Cucumis melo var. makuwa] >TYK13759.1 coiled-coil domain-containing protein 22 [Cucumis melo var. makuwa])

HSP 1 Score: 884.0 bits (2283), Expect = 6.1e-253
Identity = 469/511 (91.78%), Postives = 488/511 (95.50%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPPDLSSIRNLSPPIFFSICAQVLNRIHPQTKIPFSTSLPDS 60
           MEESQEILLNSLQKS VSIPPDLSSIRNLSPPIFFSICAQVLN+IHPQTK PFSTSLPDS
Sbjct: 1   MEESQEILLNSLQKSSVSIPPDLSSIRNLSPPIFFSICAQVLNQIHPQTKTPFSTSLPDS 60

Query: 61  VADQFKVCSEISSAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLMERLPEASDGKSKVLE 120
           VADQFKVCSEI+SAIKDLGFIGDISFHKFLYPSEEDLYKLIRFL+ERLPEASDGKSKVLE
Sbjct: 61  VADQFKVCSEIASAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120

Query: 121 DGDGRKERKVDTSRCYYVENQSNKIDTNDSVSTHQKVENKLADLNIIAEETKSPDSIVDR 180
           DGDGRKE K +TSRCYYVENQ NK DT+DSVSTHQKV++KLAD NIIAEET+SPDSI+DR
Sbjct: 121 DGDGRKE-KANTSRCYYVENQPNKTDTDDSVSTHQKVQDKLADPNIIAEETRSPDSIIDR 180

Query: 181 FSDFCLVRKSSGEAAMVDNLVNAPKDQSEVSGNESVGLQGNNGHEAGTYIQKTFEDQEIR 240
           FSDFCL+RKS GEA MVDNLVNAPKD SEV GNESVGLQGNN HE GTYIQKTFEDQ I 
Sbjct: 181 FSDFCLIRKSLGEAPMVDNLVNAPKDHSEVCGNESVGLQGNNRHEVGTYIQKTFEDQAIG 240

Query: 241 PLEEVSTNASELKHFQVELEMLKAVTRIVFDDNHSIEFHLQQLEEQINSRKLNLLEMKSQ 300
           PLEEV+TNASELKHFQVELE L+AVTRIVFDDNHSIEFHLQQLEEQINS+KL+LLEMKSQ
Sbjct: 241 PLEEVTTNASELKHFQVELERLEAVTRIVFDDNHSIEFHLQQLEEQINSKKLDLLEMKSQ 300

Query: 301 WDAERELLENKRRSLQESLCASNPKAQEKLQKLREFESEKELIELEIRRREEENSNIAID 360
           WDAERELLE KRRSLQESLCA NP AQEKL+KLREFESEK+LIELEIRRREEENSNI ID
Sbjct: 301 WDAERELLEIKRRSLQESLCARNPMAQEKLKKLREFESEKKLIELEIRRREEENSNITID 360

Query: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420
           LKKQPKQ SRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD
Sbjct: 361 LKKQPKQSSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420

Query: 421 ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480
           ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSL+
Sbjct: 421 ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLD 480

Query: 481 FNKLQADLDAIRRENSRLEQHLCHIKPIDKV 512
           FNKLQADLDAIRRENS LEQHLCH KP+D+V
Sbjct: 481 FNKLQADLDAIRRENSHLEQHLCHKKPVDEV 510

BLAST of PI0004871 vs. NCBI nr
Match: XP_038903178.1 (coiled-coil domain-containing protein 22 isoform X2 [Benincasa hispida])

HSP 1 Score: 840.5 bits (2170), Expect = 7.7e-240
Identity = 445/506 (87.94%), Postives = 474/506 (93.68%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPPDLSSIRNLSPPIFFSICAQVLNRIHPQTKIPFSTSLPDS 60
           MEESQEILLNSLQKSGVSIPPD+SSIRNL+PP+ FSICAQVLNRIH QTKIPFS SLPDS
Sbjct: 1   MEESQEILLNSLQKSGVSIPPDISSIRNLTPPLLFSICAQVLNRIHLQTKIPFSISLPDS 60

Query: 61  VADQFKVCSEISSAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLMERLPEASDGKSKVLE 120
           VADQFK+CSEI+SAIKDLGFIGD+SFHKFLYPSEEDLYKLIRFL+ERLPEASDGKSKVLE
Sbjct: 61  VADQFKICSEIASAIKDLGFIGDMSFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120

Query: 121 DGDGRKERKVDTSRCYYVENQSNKIDTNDSVSTHQKVENKLADLNIIAEETKSPDSIVDR 180
           DGDGRKE K DTS+CYYVE+ S+K++T+DSV THQKVENK ADLNII+EETKS +SIV R
Sbjct: 121 DGDGRKETKADTSKCYYVEDPSDKVNTDDSVFTHQKVENKRADLNIISEETKSSNSIVGR 180

Query: 181 FSDFCLVRKSSGEAAMVDNLVNAPKDQSEVSGNESVGLQGNNGHEAGTYIQKTFEDQEIR 240
           FSD CLV++S GEAAM DNLVNAPKDQSEVSGNESV LQGNN HE G+Y +KT EDQEIR
Sbjct: 181 FSDLCLVQQSLGEAAMADNLVNAPKDQSEVSGNESVALQGNNRHEVGSYTEKTCEDQEIR 240

Query: 241 PLEEVSTNASELKHFQVELEMLKAVTRIVFDDNHSIEFHLQQLEEQINSRKLNLLEMKSQ 300
           PLEEV++ ASEL+H QVELE LKAVTRIVFD+N SIEFHLQQLEEQINSR LNL  MKSQ
Sbjct: 241 PLEEVTSKASELEHLQVELERLKAVTRIVFDENQSIEFHLQQLEEQINSRNLNLRAMKSQ 300

Query: 301 WDAERELLENKRRSLQESLCASNPKAQEKLQKLREFESEKELIELEIRRREEENSNIAID 360
           WDAERELLENKR+SLQ+SLCASNPKAQEKLQKLREFESEK+LIE EIRRREEENSN+A D
Sbjct: 301 WDAERELLENKRQSLQDSLCASNPKAQEKLQKLREFESEKKLIESEIRRREEENSNLATD 360

Query: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420
           +KKQPKQL RRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD
Sbjct: 361 IKKQPKQLPRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420

Query: 421 ELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480
           ELV REAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN
Sbjct: 421 ELVFREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLN 480

Query: 481 FNKLQADLDAIRRENSRLEQHLCHIK 507
           F KLQADLDAIRRENS LEQHLC+ K
Sbjct: 481 FTKLQADLDAIRRENSYLEQHLCYKK 506

BLAST of PI0004871 vs. NCBI nr
Match: XP_038903177.1 (coiled-coil domain-containing protein 22 isoform X1 [Benincasa hispida])

HSP 1 Score: 835.9 bits (2158), Expect = 1.9e-238
Identity = 445/507 (87.77%), Postives = 474/507 (93.49%), Query Frame = 0

Query: 1   MEESQEILLNSLQKSGVSIPPDLSSIRNLSPPIFFSICAQVLNRIHPQTKIPFSTSLPDS 60
           MEESQEILLNSLQKSGVSIPPD+SSIRNL+PP+ FSICAQVLNRIH QTKIPFS SLPDS
Sbjct: 1   MEESQEILLNSLQKSGVSIPPDISSIRNLTPPLLFSICAQVLNRIHLQTKIPFSISLPDS 60

Query: 61  VADQFKVCSEISSAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLMERLPEASDGKSKVLE 120
           VADQFK+CSEI+SAIKDLGFIGD+SFHKFLYPSEEDLYKLIRFL+ERLPEASDGKSKVLE
Sbjct: 61  VADQFKICSEIASAIKDLGFIGDMSFHKFLYPSEEDLYKLIRFLVERLPEASDGKSKVLE 120

Query: 121 DGDGRKERKVDTSRCYYVENQSNKIDTNDSVSTHQKVENKLADLNIIAEETKSPDSIVDR 180
           DGDGRKE K DTS+CYYVE+ S+K++T+DSV THQKVENK ADLNII+EETKS +SIV R
Sbjct: 121 DGDGRKETKADTSKCYYVEDPSDKVNTDDSVFTHQKVENKRADLNIISEETKSSNSIVGR 180

Query: 181 FSDFCLVRKSSGEAAMVDNLVNAPKDQSEVSGNESVGLQGNNGHEAGTYIQKTFEDQEIR 240
           FSD CLV++S GEAAM DNLVNAPKDQSEVSGNESV LQGNN HE G+Y +KT EDQEIR
Sbjct: 181 FSDLCLVQQSLGEAAMADNLVNAPKDQSEVSGNESVALQGNNRHEVGSYTEKTCEDQEIR 240

Query: 241 PLEEVSTNASELKHFQVELEMLKAVTRIVFDDNHSIEFHLQQLEEQINSRKLNLLEMKSQ 300
           PLEEV++ ASEL+H QVELE LKAVTRIVFD+N SIEFHLQQLEEQINSR LNL  MKSQ
Sbjct: 241 PLEEVTSKASELEHLQVELERLKAVTRIVFDENQSIEFHLQQLEEQINSRNLNLRAMKSQ 300

Query: 301 WDAERELLENKRRSLQESLCASNPKAQEKLQKLREFESEKELIELEIRRREEENSNIAID 360
           WDAERELLENKR+SLQ+SLCASNPKAQEKLQKLREFESEK+LIE EIRRREEENSN+A D
Sbjct: 301 WDAERELLENKRQSLQDSLCASNPKAQEKLQKLREFESEKKLIESEIRRREEENSNLATD 360

Query: 361 LKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420
           +KKQPKQL RRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD
Sbjct: 361 IKKQPKQLPRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVD 420

Query: 421 ELVL-REAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSL 480
           ELV  REAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSL
Sbjct: 421 ELVFSREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASASRSL 480

Query: 481 NFNKLQADLDAIRRENSRLEQHLCHIK 507
           NF KLQADLDAIRRENS LEQHLC+ K
Sbjct: 481 NFTKLQADLDAIRRENSYLEQHLCYKK 507

BLAST of PI0004871 vs. TAIR 10
Match: AT1G55830.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF812 (InterPro:IPR008530). )

HSP 1 Score: 350.1 bits (897), Expect = 2.9e-96
Identity = 216/504 (42.86%), Postives = 321/504 (63.69%), Query Frame = 0

Query: 2   EESQEILLNSLQKSGVSIPPDLSSIRNLSPPIFFSICAQVLNRIHPQTKIPFSTSLPDSV 61
           EES++IL+ +L +SGVSIP D +S+   +P    SICAQ+LN I P     FS  LPDS+
Sbjct: 3   EESRDILMTTLIESGVSIPGDFTSVSEFTPEALVSICAQLLNLIDPSAS--FSDELPDSL 62

Query: 62  ADQFKVCSEISSAIKDLGFIGDISFHKFLYPSEEDLYKLIRFLMERLPEASDGKSKVLED 121
            ++F++C++I+ ++K+LG+I D+S++KFL+PSE+D Y+L+RFL+ERL E S+G+ K L  
Sbjct: 63  PERFRICTDIAHSVKNLGYINDMSYYKFLHPSEDDSYRLVRFLVERLSEISEGR-KTLTA 122

Query: 122 GDGRKERKVDTSRCYYVENQSNKIDTNDSVSTH-QKVENKLADLNIIAEETKSPDSIVDR 181
           GD     K++T R    +   N+ D +++   H QKVE  L DL + +E++ S DS    
Sbjct: 123 GDIASRPKMETFRDISDDMMVNE-DKDETFDMHIQKVEAVLKDLTMTSEKSHSSDS---- 182

Query: 182 FSDFCLVRKSSGEAAMVDNLVNAPKD--QSEVSGNESVGLQGNNGHE--AGTYIQKTFED 241
                L + +S          + P    +S++S  +S   + N+  +     Y     ++
Sbjct: 183 -----LAKNTSANVDFSSQKTDDPVTDVRSDLSLRDSSRCEENSYEDPFETNYETVELQN 242

Query: 242 QEIRPLEEVSTNASELKHFQVELEMLKAVTRIVFDDNHSIEFHLQQLEEQINSRKLNLLE 301
           Q    LEE+ + +S+L   + ELE+L+     + DD      +L+QL +Q+  ++ N+++
Sbjct: 243 QHDVLLEELESGSSQLCSLESELELLQMAAERLLDDKKPGGSYLEQLNQQLVVKRCNIMD 302

Query: 302 MKSQWDAERELLENKRRSLQESLCASNPKAQEKLQKLREFESEKELIELEIRRREEENSN 361
           +K QWD  R  LE K+  L + L    P+A+EK  KLR+ E + + +  EI++RE+E  N
Sbjct: 303 LKKQWDDVRLTLETKKLLLLDQLHVEEPEAKEKFHKLRKTELDLQSLSSEIQKREDERCN 362

Query: 362 IAIDLKKQPKQLSRRSYINRVKEITKNSRKQDADIDRILKETRELQLESNNIQERLHRTY 421
           +  +L++QPK   R+SYI+ +KEITKNSRK D DI RI  ETRELQLE N+IQERLHR+Y
Sbjct: 363 LYNELERQPKAAPRKSYIHGIKEITKNSRKLDTDIQRISGETRELQLEKNSIQERLHRSY 422

Query: 422 AVVDELVLREAKKDGIGKQAHKLLTSIHENFGEISNKILSTDRLRRETAEYEKKIAASAS 481
           AVVDE+V RE KKD   +Q +KLLTSIH  F +IS KIL TDR RRET +YEKK+ +  +
Sbjct: 423 AVVDEMVTREVKKDPAVRQVYKLLTSIHSIFEQISEKILMTDRFRRETVDYEKKLGSITA 482

Query: 482 RSLNFNKLQADLDAIRRENSRLEQ 501
           R ++  KLQADLDAIR+EN  L++
Sbjct: 483 RGMSLEKLQADLDAIRKENESLKK 493

BLAST of PI0004871 vs. TAIR 10
Match: AT1G55830.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF812 (InterPro:IPR008530); Has 10940 Blast hits to 7289 proteins in 653 species: Archae - 228; Bacteria - 791; Metazoa - 6174; Fungi - 805; Plants - 342; Viruses - 19; Other Eukaryotes - 2581 (source: NCBI BLink). )

HSP 1 Score: 269.2 bits (687), Expect = 6.6e-72
Identity = 176/418 (42.11%), Postives = 258/418 (61.72%), Query Frame = 0

Query: 88  KFLYPSEEDLYKLIRFLMERLPEASDGKSKVLEDGDGRKERKVDTSRCYYVENQSNKIDT 147
           +FL+PSE+D Y+L+RFL+ERL E S+G+ K L  GD     K++T R    +   N+ D 
Sbjct: 6   QFLHPSEDDSYRLVRFLVERLSEISEGR-KTLTAGDIASRPKMETFRDISDDMMVNE-DK 65

Query: 148 NDSVSTH-QKVENKLADLNIIAEETKSPDSIVDRFSDFCLVRKSSGEAAMVDNLVNAPKD 207
           +++   H QKVE  L DL + +E++ S DS         L + +S          + P  
Sbjct: 66  DETFDMHIQKVEAVLKDLTMTSEKSHSSDS---------LAKNTSANVDFSSQKTDDPVT 125

Query: 208 --QSEVSGNESVGLQGNNGHE--AGTYIQKTFEDQEIRPLEEVSTNASELKHFQVELEML 267
             +S++S  +S   + N+  +     Y     ++Q    LEE+ + +S+L   + ELE+L
Sbjct: 126 DVRSDLSLRDSSRCEENSYEDPFETNYETVELQNQHDVLLEELESGSSQLCSLESELELL 185

Query: 268 KAVTRIVFDDNHSIEFHLQQLEEQINSRKLNLLEMKSQWDAERELLENKRRSLQESLCAS 327
           +     + DD      +L+QL +Q+  ++ N++++K QWD  R  LE K+  L + L   
Sbjct: 186 QMAAERLLDDKKPGGSYLEQLNQQLVVKRCNIMDLKKQWDDVRLTLETKKLLLLDQLHVE 245

Query: 328 NPKAQEKLQKLREFESEKELIELEIRRREEENSNIAIDLKKQPKQLSRRSYINRVKEITK 387
            P+A+EK  KLR+ E + + +  EI++RE+E  N+  +L++QPK   R+SYI+ +KEITK
Sbjct: 246 EPEAKEKFHKLRKTELDLQSLSSEIQKREDERCNLYNELERQPKAAPRKSYIHGIKEITK 305

Query: 388 NSRKQDADIDRILKETRELQLESNNIQERLHRTYAVVDELVLREAKKDGIGKQAHKLLTS 447
           NSRK D DI RI  ETRELQLE N+IQERLHR+YAVVDE+V RE KKD   +Q +KLLTS
Sbjct: 306 NSRKLDTDIQRISGETRELQLEKNSIQERLHRSYAVVDEMVTREVKKDPAVRQVYKLLTS 365

Query: 448 IHENFGEISNKILSTDRLRRETAEYEKKIAASASRSLNFNKLQADLDAIRRENSRLEQ 501
           IH  F +IS KIL TDR RRET +YEKK+ +  +R ++  KLQADLDAIR+EN  L++
Sbjct: 366 IHSIFEQISEKILMTDRFRRETVDYEKKLGSITARGMSLEKLQADLDAIRKENESLKK 412