PI0004715 (gene) Melon (PI 482460) v1

Overview
NamePI0004715
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionEf-hand calcium binding protein
Locationchr08: 3800509 .. 3803827 (+)
RNA-Seq ExpressionPI0004715
SyntenyPI0004715
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAAAATGTCAGGTTACCCAAATCAACCTTCCGGTTACGGTTACGGTTATGGCTACGGCGGCACCGGTGGCTACGGAGCTCCACCACCGACCGCTCAGCCTTACAATTCTTCAGCCTACGGACATCCACAGTCCCAGACTCAGCAGCCGTACGCTCAAGTGGCCGCGCCATATGGCGCACCGTCCGCACCGTACGGGTCAGCTCAGGGCGAAAAACCGCCCAAGGACAAGCCTAATCAGTCTTACGGCGGTCCGGTGGTGGTCATCAGTCTTACGGCGGACCAGCCGGTGGTGGTCATGGTTATCCACCACCTTCTGCTTATGGAAGCCCTTTTGCTTCACTTGCCATCGGCTTTTCCGCCTGGTACTGATCCGAGTATCGTTGCTTGTTTTCAAGTAGCCGATCAGGATGGGAGTGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTGTCATCGTATAATCAGAAGTTTAGCATCAGAACTGTTCATCTTCTCATGTACCATTTCACCAACACGAATACCAGGAAGATCGGTAATCTCACTTTTCCCTCTATTGCCTTTTTGTTTGCTCTGGAAATAAGAAATTTCTTGTGGTTTTCTTCAAGTTTTTGGAATCAACAACAGTAACGATGGTAAAAAAAAAAAGAGAGAATCTGTAGGAATCTGGAGGGCCCAAATAGAACAAAAAGACAAACAGATAGGTTAATGGGGGCAGGGGCGGTCATGATTGAGGCATCTACTACTATTAGTTTCAACTGATTTTAATCGAAAAATGAAATAGAAGCTTTCAGAGATAAATGTCTGATTTTAACTTGAACGGATTGGAACAGTGCCATTCATTGTCATTTGTCAAAACTTTTAATAGAATTTTCATATAATTGCATAAGATAGTACGATTTTAATCTTCGACTCAAGAGACGAGTACATATAAATTCCAGTTGAAGCTATGCTAATTTGACATATAATTGCAGAGAATTGCAATGTGCCTTATTTGTGATTATGATTATTCTTGTTACTGTTAGCTGATTAGTTCACGATGCTTACAAATCGTACCTATGACTATGAGGTATTCATATTCTCATATATTCGTATAGAACTTACCAGAAGATACCATCATATTCATACCATATACTTTCTGTTTTTCTTTTTCTTTTAATCCTATCTGTACAAGGATTACCTCCGTGTTATTGCCTGGGATTAGTTGGTGAAGTATTTTATGTTTTATTTTAAATTTGTCTGGTCAAATTGCAAACCTGCTAATTGGATATATTAGTCGTGGTGGTTGCACGAATTTCACTATTTTCAAACTTGTGGTTAAAAGACTAAAGTTATTGACTTTTCAAAACTCAGAAGCTTTTGGGATATTACAGTCTCGTTATTATGTAAGTATATTTTGAAATGATGTAAAACAGTGATGTTTTATGCTTCAAATTAGAGCTATAGTTAAATTGTTAATTCTTCATTCTGTTCAAATCATATTATTATCATTAACTTTTTATGCATTTGACCTTGAGCAACCTGAATCTGGAAATCATGTTTTATACCTTTCTTTTGACGTTGTCTTTTGCATTTTGTTTCTGAAAAACAGGACCTAAGGAGTTCATTTCTCTGTTTTATGGACTTCAGAGCTGGAGGGTAAGTTGAAGACTTATTATTATTTTAGCCAACATTATTTTTTGGTGTAATTCGTTTAAACTGAATATTTGTTGAATCTTTTTCAGGGTATTTTTGAGAGGTTTGATTCAGACAGAAGTGGAAAAATTGATTCCAATGAGTTGCGAGAAGCACTTTTGAGTTTGGGCTTTGCAGTCTCACCAATGGTCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGTGGTGGGAAAAGCAAGGCCATTGAATACGATAATTTTATTGAGTACGTTTGTCTTCTTTACCCTTGATTTTATGGTGGTACTCTTTTTAAAATGAGAGGAGTTATGCTTTAAATGTTAAGTTTTCACTTTTCATTAATCACTGTTCTTATTCTTTTTGTTTTTTCTTTTTGCAAATGCAGGTGTTGTCTTACGGTCAAGGTGAGTTCCTGACTATTTTTGTTTGCATGTCTTTTGTATATTAAAACATGTACTGTTTAAAGGCAATGGCATCATCTTTGAAGCCAAATGCTTATTCCGCAATCTGTACTTAGGATTGATTTTGAAATGGTAAAAATCACTTTTGTCATGCTCAAAATCACTGCGAAATACACCTTTAGTAATTTAAAATTAGTTAAATATTTAATTTTATGCTTGTACTTGCAATTTTCATTTCATCAATACTGGTTTTGAATCATTAAAAGCATGTTAAAAGCATGTTTCATAATGATTTTAAATTAAAAATGACAAAAGTGATTTTAACCGTTTTAGAATCACTCACAAACATGCATAGTTGTACACATTTTCCTAGTTGTCTTCATTATTTTCCACTCATGTTGATGCCTATGAATGTGCCGAACGTCTGCTACACCCAGCTTGACTTCTGTGGCTGCCAAGTAGTTACTTATAATCCAATTTCACTATGAGATATTGCTGTTGTTATTTTCATTTTTTTCTCCCTTCCATTTTTCACACACTGATAGTTTAAGCTCCTTGTATGAACCTTTAACTCAATTCTCCAGCACCGAAACTGTTTTGATTTTTACGAATTCCAGAAACATTTTGTTTGCGATGGGGAGAAAGAATAGAGTAAAACTGAAGTGATTTTGATGTAATATTTTACAGGGATTGACTGAAAAATTCAAGGAAAAGGACACTACATACTCTGGTTCTGCAACCTTCAGTTATGAGACTTTCATGTTGACTGTGTTACCATTTCTCATTGCCTGAGGAGTAAGCCTTTGTTTCGAGCACGTTTGTTCATTGTAAAGCTTGAGTTTTCTTTGGCTTTTTCTTTTGATTTCCGTTATGTTTGATAGAATCTATTAATGTAGCCTCATCCTTATCAATGTTATAAGTTATTTTCTTTGTATAAATTGGCTATCTTAAGTATTCAATGATAGTTCTATGAATATGTCAACTGTTTACCTGTTTCCCCTACTGGATGTCCAATTTGGCATTTGACAGTAAAAGTTAGGTCGTTGAATCATGTATAAATATTGTGGATGAAGTTTGGACAGTTAAATTGCAAGTGATTCTGGGTTGATTTCTTTTTGAATAAATGTACAAGAATCGAACATCTTGATTTCTTGTCATTTGATTTGTACTATTTACCTGTAAGAATACAGAAGTGTTTATGTAATTGCAAATAATAGTCCTTTGGACAGTTTCAGTTTTCAAAAGAAGAAAATTGATTTTTTTTTT

mRNA sequence

GCAAAATGTCAGGTTACCCAAATCAACCTTCCGGTTACGGTTACGGTTATGGCTACGGCGGCACCGGTGGCTACGGAGCTCCACCACCGACCGCTCAGCCTTACAATTCTTCAGCCTACGGACATCCACAGTCCCAGACTCAGCAGCCGTACGCTCAAGTGGCCGCGCCATATGGCGCACCGTCCGCACCGTACGGGTCAGCTCAGGGCGAAAAACCGCCCAAGGACAAGCCTAATCAGTCTTACGGCGGTCCGGTGGTGGTCATCAGTCTTACGGCGGACCAGCCGGTGGTGGTCATGGTTATCCACCACCTTCTGCTTATGGAAGCCCTTTTGCTTCACTTGCCATCGGCTTTTCCGCCTGGTACTGATCCGAGTATCGTTGCTTGTTTTCAAGTAGCCGATCAGGATGGGAGTGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTGTCATCGTATAATCAGAAGTTTAGCATCAGAACTGTTCATCTTCTCATGTACCATTTCACCAACACGAATACCAGGAAGATCGGACCTAAGGAGTTCATTTCTCTGTTTTATGGACTTCAGAGCTGGAGGGGTATTTTTGAGAGGTTTGATTCAGACAGAAGTGGAAAAATTGATTCCAATGAGTTGCGAGAAGCACTTTTGAGTTTGGGCTTTGCAGTCTCACCAATGGTCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGTGGTGGGAAAAGCAAGGCCATTGAATACGATAATTTTATTGAGTGTTGTCTTACGGTCAAGGGATTGACTGAAAAATTCAAGGAAAAGGACACTACATACTCTGGTTCTGCAACCTTCAGTTATGAGACTTTCATGTTGACTGTGTTACCATTTCTCATTGCCTGAGGAGTAAGCCTTTGTTTCGAGCACGTTTGTTCATTGTAAAGCTTGAGTTTTCTTTGGCTTTTTCTTTTGATTTCCGTTATGTTTGATAGAATCTATTAATGTAGCCTCATCCTTATCAATGTTATAAGTTATTTTCTTTGTATAAATTGGCTATCTTAAGTATTCAATGATAGTTCTATGAATATGTCAACTGTTTACCTGTTTCCCCTACTGGATGTCCAATTTGGCATTTGACAGTAAAAGTTAGGTCGTTGAATCATGTATAAATATTGTGGATGAAGTTTGGACAGTTAAATTGCAAGTGATTCTGGGTTGATTTCTTTTTGAATAAATGTACAAGAATCGAACATCTTGATTTCTTGTCATTTGATTTGTACTATTTACCTGTAAGAATACAGAAGTGTTTATGTAATTGCAAATAATAGTCCTTTGGACAGTTTCAGTTTTCAAAAGAAGAAAATTGATTTTTTTTTT

Coding sequence (CDS)

ATGTCAGGTTACCCAAATCAACCTTCCGGTTACGGTTACGGTTATGGCTACGGCGGCACCGGTGGCTACGGAGCTCCACCACCGACCGCTCAGCCTTACAATTCTTCAGCCTACGGACATCCACAGTCCCAGACTCAGCAGCCGTACGCTCAAGTGGCCGCGCCATATGGCGCACCGTCCGCACCGTACGGGTCAGCTCAGGGCGAAAAACCGCCCAAGGACAAGCCTAATCAGTCTTACGGCGGTCCGGTGGTGGTCATCAGTCTTACGGCGGACCAGCCGGTGGTGGTCATGGTTATCCACCACCTTCTGCTTATGGAAGCCCTTTTGCTTCACTTGCCATCGGCTTTTCCGCCTGGTACTGATCCGAGTATCGTTGCTTGTTTTCAAGTAGCCGATCAGGATGGGAGTGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTGTCATCGTATAATCAGAAGTTTAGCATCAGAACTGTTCATCTTCTCATGTACCATTTCACCAACACGAATACCAGGAAGATCGGACCTAAGGAGTTCATTTCTCTGTTTTATGGACTTCAGAGCTGGAGGGGTATTTTTGAGAGGTTTGATTCAGACAGAAGTGGAAAAATTGATTCCAATGAGTTGCGAGAAGCACTTTTGAGTTTGGGCTTTGCAGTCTCACCAATGGTCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGTGGTGGGAAAAGCAAGGCCATTGAATACGATAATTTTATTGAGTGTTGTCTTACGGTCAAGGGATTGACTGAAAAATTCAAGGAAAAGGACACTACATACTCTGGTTCTGCAACCTTCAGTTATGAGACTTTCATGTTGACTGTGTTACCATTTCTCATTGCCTGA

Protein sequence

MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAQVAAPYGAPSAPYGSAQGEKPPKDKPNQSYGGPVVVISLTADQPVVVMVIHHLLLMEALLLHLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYETFMLTVLPFLIA
Homology
BLAST of PI0004715 vs. ExPASy Swiss-Prot
Match: Q2QY10 (Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.3e-90
Identity = 195/320 (60.94%), Postives = 216/320 (67.50%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPP--------PTAQPY-----------NSSA---- 60
           M+GYP  P   G GY YGG GGYGAPP        P+A PY           +SSA    
Sbjct: 1   MAGYPPNP---GSGYPYGGAGGYGAPPPPYGSSPAPSAPPYGAKPPKEGKTSSSSAPYYG 60

Query: 61  ----YGHPQSQTQQPYAQVAAPYGAP--SAPYGSAQGEKPPKDKPNQSYGGPVVVISLTA 120
               YG P S   QPY      YGAP  S PYG+  G  PP   P  + GG         
Sbjct: 61  GGGGYGAPPS--TQPYGS-GGGYGAPPSSQPYGAPYGAPPPSSAPYGAPGG--------Y 120

Query: 121 DQPVVVMVIHHLLLMEALLLHLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSS 180
             P   +V              PSAFPPGTDP++VACFQ AD+DGSG IDDKELQ  LS 
Sbjct: 121 GSPFASLV--------------PSAFPPGTDPNVVACFQAADRDGSGMIDDKELQSALSG 180

Query: 181 YNQKFSIRTVHLLMYHFTNTNTRKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNEL 240
           Y+Q FS+RTVHLLMY FTNTN RKIGPKEF S+FY LQ+WR IFERFD DRSGKID+ EL
Sbjct: 181 YSQSFSLRTVHLLMYLFTNTNVRKIGPKEFTSVFYSLQNWRSIFERFDRDRSGKIDATEL 240

Query: 241 REALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYS 292
           R+ALLSLG++VSP VLDLLVSKFDK+GGK+KAIEYDNFIECCLTVKGLTEKFKEKDT +S
Sbjct: 241 RDALLSLGYSVSPTVLDLLVSKFDKTGGKNKAIEYDNFIECCLTVKGLTEKFKEKDTAFS 292

BLAST of PI0004715 vs. ExPASy Swiss-Prot
Match: Q8W4L0 (Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 PE=2 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 3.4e-86
Identity = 197/344 (57.27%), Postives = 220/344 (63.95%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYG---------YGGTGG----YGA----PPPTAQPYNSSAYGHPQS 60
           MSGYP  PS  GYGYG         YG TG     YG+    PPP   PY SSA     S
Sbjct: 1   MSGYP--PSSQGYGYGGNPPPPQPPYGSTGNNPPPYGSSGSNPPP---PYGSSA----SS 60

Query: 61  QTQQPYAQVAAPYGA-PSAPYGSAQGE--KPPKDKPNQS--------------------- 120
               PY    APYGA PSAPY S  G+  KP K+KP+ +                     
Sbjct: 61  PYAVPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDY 120

Query: 121 --YGGPVVVISLTADQPVVVMVIHH----------LLLMEALLLHLPSAFPPGTDPSIVA 180
             YGG                   H                    +PSAFPPGTDP+IVA
Sbjct: 121 GGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVA 180

Query: 181 CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFISLFYG 240
           CFQ AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF SLF+ 
Sbjct: 181 CFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFS 240

Query: 241 LQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYD 292
           LQ+WR IFERFD DRSG+ID+NELR+AL+SLGF+VSP++LDLLVSKFDKSGG+++AIEYD
Sbjct: 241 LQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYD 300

BLAST of PI0004715 vs. ExPASy Swiss-Prot
Match: Q9FYE4 (Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 PE=2 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 9.9e-86
Identity = 199/362 (54.97%), Postives = 219/362 (60.50%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHP--QSQTQQPYAQVAAPYGA 60
           MSGYP  P+  GYGYGYGG      PPP   PY+S     P   S T  PY   A PYGA
Sbjct: 1   MSGYP--PTSQGYGYGYGGG---NQPPPPQPPYSSGGNNPPYGSSTTSSPY---AVPYGA 60

Query: 61  ----------------------PSAPYGSAQGE--KPPKDKP-NQSYGGPVVVISLTADQ 120
                                 PSAPY  + G+  KPPK+KP    YG P    S     
Sbjct: 61  SKPQSSSSSAPTYGSSSYGAPPPSAPYAPSPGDYNKPPKEKPYGGGYGAPPPSGSSDYGS 120

Query: 121 ----PVVVMVIHHLLLMEALLLH------------------------------------- 180
               P       H     A   H                                     
Sbjct: 121 YGAGPRPSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASYGSPF 180

Query: 181 ---LPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFT 240
              +PS F PGTDP+IVACFQ ADQDGSGFIDDKELQG LSSY Q+FS+RTVHLLMY FT
Sbjct: 181 ASLIPSGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFT 240

Query: 241 NTNTRKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDL 292
           N+N  KIGPKEF +LFY LQ+WR IFER D DRSG+ID NELR+ALLSLGF+VSP+VLDL
Sbjct: 241 NSNAMKIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPVVLDL 300

BLAST of PI0004715 vs. ExPASy Swiss-Prot
Match: Q9ZQH1 (Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 PE=2 SV=2)

HSP 1 Score: 153.3 bits (386), Expect = 4.2e-36
Identity = 80/176 (45.45%), Postives = 115/176 (65.34%), Query Frame = 0

Query: 117 FPPGTDPSIVACFQVADQDGSGFIDDKELQGVLS-SYNQKFSIRTVHLLM--YHFTNTNT 176
           F P T P IV  F+ AD++ SGF+++ EL+  LS S     S RT+ LL+  Y     + 
Sbjct: 49  FSPETHPEIVRSFESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIYKIPVDSL 108

Query: 177 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSK 236
            ++GPKE++ L+  L  WR IF R+D DRSGK++S +LR+A  +LG  +   V  L+VS+
Sbjct: 109 LRLGPKEYVELWNCLAQWRAIFNRYDRDRSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQ 168

Query: 237 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYETFMLTVLPFL 290
           FD   GK+  + +D+F+EC + VKGLTEKF+E D  Y+G AT SY+ FML V+PF+
Sbjct: 169 FDDGTGKTVDLCFDSFLECGMIVKGLTEKFRENDPGYTGYATLSYDVFMLMVIPFI 224

BLAST of PI0004715 vs. ExPASy Swiss-Prot
Match: Q9UBV8 (Peflin OS=Homo sapiens OX=9606 GN=PEF1 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.9e-28
Identity = 96/286 (33.57%), Postives = 132/286 (46.15%), Query Frame = 0

Query: 2   SGYPNQP-SGYGYGYGYGGTGGYGAPP---PTAQPYNSSAYGHPQSQTQQPYAQVAAPYG 61
           S YP  P SG  YG G    GGYG P    P   P     YGHP             P G
Sbjct: 24  SYYPGPPNSGGQYGSGLPPGGGYGGPAPGGPYGPPAGGGPYGHPNPG--------MFPSG 83

Query: 62  APSAPYGSAQGEKPPKDKPNQSYGGPVVVISLTADQPVVVMVIHHLLLMEALLLHLPSAF 121
            P  PYG A    P    P  SYG         A QP                L+     
Sbjct: 84  TPGGPYGGAAPGGPYGQPPPSSYG---------AQQPG---------------LYGQGGA 143

Query: 122 PPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYN-QKFSIRTVHLLMYHFTNTNTRKI 181
           PP  DP   + FQ  D D SG+I  KEL+  L + N   F+  T  +++  F  T + +I
Sbjct: 144 PPNVDPEAYSWFQSVDSDHSGYISMKELKQALVNCNWSSFNDETCLMMINMFDKTKSGRI 203

Query: 182 GPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDK 241
               F +L+  +Q W+ +F+++D DRSG I   EL++AL  +G+ +SP    LLVS++  
Sbjct: 204 DVYGFSALWKFIQQWKNLFQQYDRDRSGSISYTELQQALSQMGYNLSPQFTQLLVSRYCP 263

Query: 242 SGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYETFM 283
               + A++ D FI+ C  ++ LTE F+EKDT   G+   S+E F+
Sbjct: 264 RSA-NPAMQLDRFIQVCTQLQVLTEAFREKDTAVQGNIRLSFEDFV 276

BLAST of PI0004715 vs. ExPASy TrEMBL
Match: A0A5D3BYW0 (Putative calcium-binding protein CML49 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold527G00390 PE=4 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.0e-133
Identity = 257/298 (86.24%), Postives = 260/298 (87.25%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAQVAAPYGAPS 60
           MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYA VAAPYG PS
Sbjct: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPYGGPS 60

Query: 61  APYGSAQGEKPPKDKPNQSYGGP-------VVVISLTADQPVVVMVIHHLLLMEALLLHL 120
           APY SAQGEKPPKDKPNQSYGG                  P      +    +      L
Sbjct: 61  APY-SAQGEKPPKDKPNQSYGGAGGGHQSYGGAAGGGHGYPPPSAYGNPFASL------L 120

Query: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180
           PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT
Sbjct: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180

Query: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSK 240
           RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELR+ALLSLGFAVSPMVLDLLVSK
Sbjct: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELRDALLSLGFAVSPMVLDLLVSK 240

Query: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYETFMLTVLPFLIA 292
           FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYE FMLTVLPFLIA
Sbjct: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 291

BLAST of PI0004715 vs. ExPASy TrEMBL
Match: A0A1S3CNE3 (probable calcium-binding protein CML49 OS=Cucumis melo OX=3656 GN=LOC103502445 PE=4 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.0e-133
Identity = 257/298 (86.24%), Postives = 260/298 (87.25%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAQVAAPYGAPS 60
           MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYA VAAPYG PS
Sbjct: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPYGGPS 60

Query: 61  APYGSAQGEKPPKDKPNQSYGGP-------VVVISLTADQPVVVMVIHHLLLMEALLLHL 120
           APY SAQGEKPPKDKPNQSYGG                  P      +    +      L
Sbjct: 61  APY-SAQGEKPPKDKPNQSYGGAGGGHQSYGGAAGGGHGYPPPSAYGNPFASL------L 120

Query: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180
           PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT
Sbjct: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180

Query: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSK 240
           RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELR+ALLSLGFAVSPMVLDLLVSK
Sbjct: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELRDALLSLGFAVSPMVLDLLVSK 240

Query: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYETFMLTVLPFLIA 292
           FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYE FMLTVLPFLIA
Sbjct: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 291

BLAST of PI0004715 vs. ExPASy TrEMBL
Match: A0A0A0L336 (Ef-hand calcium binding protein OS=Cucumis sativus OX=3659 GN=Csa_4G341560 PE=4 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 8.6e-133
Identity = 257/298 (86.24%), Postives = 258/298 (86.58%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAQVAAPYGAPS 60
           MSGYPNQPS  GYGYGYG TGGYGAPPPTAQPYNSSAYGHPQSQTQQPYA VAAPYG PS
Sbjct: 1   MSGYPNQPS--GYGYGYGSTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPYGVPS 60

Query: 61  APYGSAQGEKPPKDKPNQSYGGP-------VVVISLTADQPVVVMVIHHLLLMEALLLHL 120
           APYGSAQGEKPPKDKPNQSYGG            S     P           +      L
Sbjct: 61  APYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASL------L 120

Query: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180
           PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT
Sbjct: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180

Query: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSK 240
           RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSK
Sbjct: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSK 240

Query: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYETFMLTVLPFLIA 292
           FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYE FMLTVLPFLIA
Sbjct: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 290

BLAST of PI0004715 vs. ExPASy TrEMBL
Match: A0A5A7VQC6 (Putative calcium-binding protein CML49 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold138G00690 PE=4 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.4e-127
Identity = 251/298 (84.23%), Postives = 254/298 (85.23%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAQVAAPYGAPS 60
           MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYA VAAPYG PS
Sbjct: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPYGGPS 60

Query: 61  APYGSAQGEKPPKDKPNQSYGGP-------VVVISLTADQPVVVMVIHHLLLMEALLLHL 120
           APY SAQGEKPPKDKPNQSYGG                  P      +    +      L
Sbjct: 61  APY-SAQGEKPPKDKPNQSYGGAGGGHQSYGGAAGGGHGYPPPSAYGNPFASL------L 120

Query: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180
           PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT
Sbjct: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180

Query: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSK 240
           RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELR+ALLSLGFAVSPMVLDLLVSK
Sbjct: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELRDALLSLGFAVSPMVLDLLVSK 240

Query: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYETFMLTVLPFLIA 292
           FDKSGGKSKAIEYDNFIE      GLTEKFKEKDTTYSGSATFSYE FMLTVLPFLIA
Sbjct: 241 FDKSGGKSKAIEYDNFIE-----YGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 286

BLAST of PI0004715 vs. ExPASy TrEMBL
Match: A0A6J1HP98 (probable calcium-binding protein CML49 OS=Cucurbita maxima OX=3661 GN=LOC111466073 PE=4 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 1.1e-124
Identity = 243/293 (82.94%), Postives = 247/293 (84.30%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYG--HPQSQTQQPYAQVAAPYGA 60
           MSGYPNQPS  GYGYGYGG GGYGAPPPTAQPY SS YG  HP   +QQPYA VAAPYGA
Sbjct: 1   MSGYPNQPS--GYGYGYGGAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAAPYGA 60

Query: 61  PSAPYGSAQGEKPPKDKPNQSYGGPVVVISLTADQPVVVMVIHHLLLMEALLLHLPSAFP 120
           PSAPYGS  GEKP KDKPNQS+GG                  +           LPSAFP
Sbjct: 61  PSAPYGSNPGEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYG----SPFASLLPSAFP 120

Query: 121 PGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGP 180
           PGTDPSIV CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGP
Sbjct: 121 PGTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGP 180

Query: 181 KEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSG 240
           KEFI+LFYGLQSWRGIFERFDSDRSGKID NELREALLSLGFAVSPMVLDLLVSKFDKSG
Sbjct: 181 KEFIALFYGLQSWRGIFERFDSDRSGKIDYNELREALLSLGFAVSPMVLDLLVSKFDKSG 240

Query: 241 GKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYETFMLTVLPFLIA 292
           GKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYE FMLTVLPFLIA
Sbjct: 241 GKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 287

BLAST of PI0004715 vs. NCBI nr
Match: XP_008464601.1 (PREDICTED: probable calcium-binding protein CML49 [Cucumis melo] >TYK04310.1 putative calcium-binding protein CML49 [Cucumis melo var. makuwa])

HSP 1 Score: 486.1 bits (1250), Expect = 2.1e-133
Identity = 257/298 (86.24%), Postives = 260/298 (87.25%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAQVAAPYGAPS 60
           MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYA VAAPYG PS
Sbjct: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPYGGPS 60

Query: 61  APYGSAQGEKPPKDKPNQSYGGP-------VVVISLTADQPVVVMVIHHLLLMEALLLHL 120
           APY SAQGEKPPKDKPNQSYGG                  P      +    +      L
Sbjct: 61  APY-SAQGEKPPKDKPNQSYGGAGGGHQSYGGAAGGGHGYPPPSAYGNPFASL------L 120

Query: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180
           PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT
Sbjct: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180

Query: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSK 240
           RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELR+ALLSLGFAVSPMVLDLLVSK
Sbjct: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELRDALLSLGFAVSPMVLDLLVSK 240

Query: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYETFMLTVLPFLIA 292
           FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYE FMLTVLPFLIA
Sbjct: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 291

BLAST of PI0004715 vs. NCBI nr
Match: XP_004142653.1 (probable calcium-binding protein CML49 [Cucumis sativus] >KGN54506.1 hypothetical protein Csa_011963 [Cucumis sativus])

HSP 1 Score: 483.0 bits (1242), Expect = 1.8e-132
Identity = 257/298 (86.24%), Postives = 258/298 (86.58%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAQVAAPYGAPS 60
           MSGYPNQPS  GYGYGYG TGGYGAPPPTAQPYNSSAYGHPQSQTQQPYA VAAPYG PS
Sbjct: 1   MSGYPNQPS--GYGYGYGSTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPYGVPS 60

Query: 61  APYGSAQGEKPPKDKPNQSYGGP-------VVVISLTADQPVVVMVIHHLLLMEALLLHL 120
           APYGSAQGEKPPKDKPNQSYGG            S     P           +      L
Sbjct: 61  APYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASL------L 120

Query: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180
           PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT
Sbjct: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180

Query: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSK 240
           RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSK
Sbjct: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSK 240

Query: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYETFMLTVLPFLIA 292
           FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYE FMLTVLPFLIA
Sbjct: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 290

BLAST of PI0004715 vs. NCBI nr
Match: XP_038883807.1 (probable calcium-binding protein CML50 [Benincasa hispida])

HSP 1 Score: 473.8 bits (1218), Expect = 1.1e-129
Identity = 253/299 (84.62%), Postives = 258/299 (86.29%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQ-SQTQQPYAQVAAPYGAP 60
           MSGYPNQPSGYGYGYGYGG+GGYGAPPPTAQ YNSSAYGHPQ  QTQQPYA VAAPYGAP
Sbjct: 1   MSGYPNQPSGYGYGYGYGGSGGYGAPPPTAQVYNSSAYGHPQPQQTQQPYAPVAAPYGAP 60

Query: 61  SAPYGSAQGEKPPKDKPNQSYGGP-------VVVISLTADQPVVVMVIHHLLLMEALLLH 120
           SAPYGSA GEKPPKDKPNQSYGG            S     P      +    +      
Sbjct: 61  SAPYGSAPGEKPPKDKPNQSYGGAGGGHQSYGGAGSGGHGYPPPSAYGNPFASL------ 120

Query: 121 LPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTN 180
           LPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQ VLSSY+QKFSIRTVHLLMYHFTNTN
Sbjct: 121 LPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQRVLSSYDQKFSIRTVHLLMYHFTNTN 180

Query: 181 TRKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVS 240
           TRKIGPKEFI+LFYGLQSWRGIFERFDSDRSGKIDS ELREALLSLGFAVSPMVLDLLVS
Sbjct: 181 TRKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSKELREALLSLGFAVSPMVLDLLVS 240

Query: 241 KFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYETFMLTVLPFLIA 292
           KFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYE FMLTVLPFLIA
Sbjct: 241 KFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 293

BLAST of PI0004715 vs. NCBI nr
Match: KAA0067905.1 (putative calcium-binding protein CML49 [Cucumis melo var. makuwa])

HSP 1 Score: 464.9 bits (1195), Expect = 5.0e-127
Identity = 251/298 (84.23%), Postives = 254/298 (85.23%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAQVAAPYGAPS 60
           MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYA VAAPYG PS
Sbjct: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHPQSQTQQPYAPVAAPYGGPS 60

Query: 61  APYGSAQGEKPPKDKPNQSYGGP-------VVVISLTADQPVVVMVIHHLLLMEALLLHL 120
           APY SAQGEKPPKDKPNQSYGG                  P      +    +      L
Sbjct: 61  APY-SAQGEKPPKDKPNQSYGGAGGGHQSYGGAAGGGHGYPPPSAYGNPFASL------L 120

Query: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180
           PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT
Sbjct: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180

Query: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSK 240
           RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELR+ALLSLGFAVSPMVLDLLVSK
Sbjct: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELRDALLSLGFAVSPMVLDLLVSK 240

Query: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYETFMLTVLPFLIA 292
           FDKSGGKSKAIEYDNFIE      GLTEKFKEKDTTYSGSATFSYE FMLTVLPFLIA
Sbjct: 241 FDKSGGKSKAIEYDNFIE-----YGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 286

BLAST of PI0004715 vs. NCBI nr
Match: XP_022966416.1 (probable calcium-binding protein CML49 [Cucurbita maxima])

HSP 1 Score: 456.1 bits (1172), Expect = 2.3e-124
Identity = 243/293 (82.94%), Postives = 247/293 (84.30%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYG--HPQSQTQQPYAQVAAPYGA 60
           MSGYPNQPS  GYGYGYGG GGYGAPPPTAQPY SS YG  HP   +QQPYA VAAPYGA
Sbjct: 1   MSGYPNQPS--GYGYGYGGAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAAPYGA 60

Query: 61  PSAPYGSAQGEKPPKDKPNQSYGGPVVVISLTADQPVVVMVIHHLLLMEALLLHLPSAFP 120
           PSAPYGS  GEKP KDKPNQS+GG                  +           LPSAFP
Sbjct: 61  PSAPYGSNPGEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYG----SPFASLLPSAFP 120

Query: 121 PGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGP 180
           PGTDPSIV CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGP
Sbjct: 121 PGTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGP 180

Query: 181 KEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSG 240
           KEFI+LFYGLQSWRGIFERFDSDRSGKID NELREALLSLGFAVSPMVLDLLVSKFDKSG
Sbjct: 181 KEFIALFYGLQSWRGIFERFDSDRSGKIDYNELREALLSLGFAVSPMVLDLLVSKFDKSG 240

Query: 241 GKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYETFMLTVLPFLIA 292
           GKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYE FMLTVLPFLIA
Sbjct: 241 GKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 287

BLAST of PI0004715 vs. TAIR 10
Match: AT3G10300.3 (Calcium-binding EF-hand family protein )

HSP 1 Score: 319.7 bits (818), Expect = 2.4e-87
Identity = 197/344 (57.27%), Postives = 220/344 (63.95%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYG---------YGGTGG----YGA----PPPTAQPYNSSAYGHPQS 60
           MSGYP  PS  GYGYG         YG TG     YG+    PPP   PY SSA     S
Sbjct: 1   MSGYP--PSSQGYGYGGNPPPPQPPYGSTGNNPPPYGSSGSNPPP---PYGSSA----SS 60

Query: 61  QTQQPYAQVAAPYGA-PSAPYGSAQGE--KPPKDKPNQS--------------------- 120
               PY    APYGA PSAPY S  G+  KP K+KP+ +                     
Sbjct: 61  PYAVPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDY 120

Query: 121 --YGGPVVVISLTADQPVVVMVIHH----------LLLMEALLLHLPSAFPPGTDPSIVA 180
             YGG                   H                    +PSAFPPGTDP+IVA
Sbjct: 121 GGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVA 180

Query: 181 CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFISLFYG 240
           CFQ AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF SLF+ 
Sbjct: 181 CFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFS 240

Query: 241 LQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYD 292
           LQ+WR IFERFD DRSG+ID+NELR+AL+SLGF+VSP++LDLLVSKFDKSGG+++AIEYD
Sbjct: 241 LQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYD 300

BLAST of PI0004715 vs. TAIR 10
Match: AT5G04170.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 318.2 bits (814), Expect = 7.1e-87
Identity = 199/362 (54.97%), Postives = 219/362 (60.50%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYGYGGTGGYGAPPPTAQPYNSSAYGHP--QSQTQQPYAQVAAPYGA 60
           MSGYP  P+  GYGYGYGG      PPP   PY+S     P   S T  PY   A PYGA
Sbjct: 1   MSGYP--PTSQGYGYGYGGG---NQPPPPQPPYSSGGNNPPYGSSTTSSPY---AVPYGA 60

Query: 61  ----------------------PSAPYGSAQGE--KPPKDKP-NQSYGGPVVVISLTADQ 120
                                 PSAPY  + G+  KPPK+KP    YG P    S     
Sbjct: 61  SKPQSSSSSAPTYGSSSYGAPPPSAPYAPSPGDYNKPPKEKPYGGGYGAPPPSGSSDYGS 120

Query: 121 ----PVVVMVIHHLLLMEALLLH------------------------------------- 180
               P       H     A   H                                     
Sbjct: 121 YGAGPRPSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASYGSPF 180

Query: 181 ---LPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFT 240
              +PS F PGTDP+IVACFQ ADQDGSGFIDDKELQG LSSY Q+FS+RTVHLLMY FT
Sbjct: 181 ASLIPSGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFT 240

Query: 241 NTNTRKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDL 292
           N+N  KIGPKEF +LFY LQ+WR IFER D DRSG+ID NELR+ALLSLGF+VSP+VLDL
Sbjct: 241 NSNAMKIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPVVLDL 300

BLAST of PI0004715 vs. TAIR 10
Match: AT3G10300.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 251.9 bits (642), Expect = 6.2e-67
Identity = 163/304 (53.62%), Postives = 184/304 (60.53%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYG---------YGGTGG----YGA----PPPTAQPYNSSAYGHPQS 60
           MSGYP  PS  GYGYG         YG TG     YG+    PPP   PY SSA     S
Sbjct: 1   MSGYP--PSSQGYGYGGNPPPPQPPYGSTGNNPPPYGSSGSNPPP---PYGSSA----SS 60

Query: 61  QTQQPYAQVAAPYGA-PSAPYGSAQGE--KPPKDKPNQS--------------------- 120
               PY    APYGA PSAPY S  G+  KP K+KP+ +                     
Sbjct: 61  PYAVPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDY 120

Query: 121 --YGGPVVVISLTADQPVVVMVIHH----------LLLMEALLLHLPSAFPPGTDPSIVA 180
             YGG                   H                    +PSAFPPGTDP+IVA
Sbjct: 121 GGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVA 180

Query: 181 CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFISLFYG 240
           CFQ AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF SLF+ 
Sbjct: 181 CFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFS 240

Query: 241 LQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYD 252
           LQ+WR IFERFD DRSG+ID+NELR+AL+SLGF+VSP++LDLLVSKFDKSGG+++AIEYD
Sbjct: 241 LQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYD 295

BLAST of PI0004715 vs. TAIR 10
Match: AT3G10300.4 (Calcium-binding EF-hand family protein )

HSP 1 Score: 251.9 bits (642), Expect = 6.2e-67
Identity = 163/304 (53.62%), Postives = 184/304 (60.53%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYG---------YGGTGG----YGA----PPPTAQPYNSSAYGHPQS 60
           MSGYP  PS  GYGYG         YG TG     YG+    PPP   PY SSA     S
Sbjct: 1   MSGYP--PSSQGYGYGGNPPPPQPPYGSTGNNPPPYGSSGSNPPP---PYGSSA----SS 60

Query: 61  QTQQPYAQVAAPYGA-PSAPYGSAQGE--KPPKDKPNQS--------------------- 120
               PY    APYGA PSAPY S  G+  KP K+KP+ +                     
Sbjct: 61  PYAVPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDY 120

Query: 121 --YGGPVVVISLTADQPVVVMVIHH----------LLLMEALLLHLPSAFPPGTDPSIVA 180
             YGG                   H                    +PSAFPPGTDP+IVA
Sbjct: 121 GGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVA 180

Query: 181 CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFISLFYG 240
           CFQ AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF SLF+ 
Sbjct: 181 CFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFS 240

Query: 241 LQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEYD 252
           LQ+WR IFERFD DRSG+ID+NELR+AL+SLGF+VSP++LDLLVSKFDKSGG+++AIEYD
Sbjct: 241 LQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYD 295

BLAST of PI0004715 vs. TAIR 10
Match: AT3G10300.5 (Calcium-binding EF-hand family protein )

HSP 1 Score: 247.3 bits (630), Expect = 1.5e-65
Identity = 163/305 (53.44%), Postives = 184/305 (60.33%), Query Frame = 0

Query: 1   MSGYPNQPSGYGYGYG---------YGGTGG----YGA----PPPTAQPYNSSAYGHPQS 60
           MSGYP  PS  GYGYG         YG TG     YG+    PPP   PY SSA     S
Sbjct: 1   MSGYP--PSSQGYGYGGNPPPPQPPYGSTGNNPPPYGSSGSNPPP---PYGSSA----SS 60

Query: 61  QTQQPYAQVAAPYGA-PSAPYGSAQGE--KPPKDKPNQS--------------------- 120
               PY    APYGA PSAPY S  G+  KP K+KP+ +                     
Sbjct: 61  PYAVPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDY 120

Query: 121 --YGGPVVVISLTADQPVVVMVIHH----------LLLMEALLLHLPSAFPPGTDPSIVA 180
             YGG                   H                    +PSAFPPGTDP+IVA
Sbjct: 121 GGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVA 180

Query: 181 CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIG-PKEFISLFY 240
           CFQ AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIG PKEF SLF+
Sbjct: 181 CFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGRPKEFTSLFF 240

Query: 241 GLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSKFDKSGGKSKAIEY 252
            LQ+WR IFERFD DRSG+ID+NELR+AL+SLGF+VSP++LDLLVSKFDKSGG+++AIEY
Sbjct: 241 SLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEY 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q2QY101.3e-9060.94Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2... [more]
Q8W4L03.4e-8657.27Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 ... [more]
Q9FYE49.9e-8654.97Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 ... [more]
Q9ZQH14.2e-3645.45Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 ... [more]
Q9UBV81.9e-2833.57Peflin OS=Homo sapiens OX=9606 GN=PEF1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BYW01.0e-13386.24Putative calcium-binding protein CML49 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CNE31.0e-13386.24probable calcium-binding protein CML49 OS=Cucumis melo OX=3656 GN=LOC103502445 P... [more]
A0A0A0L3368.6e-13386.24Ef-hand calcium binding protein OS=Cucumis sativus OX=3659 GN=Csa_4G341560 PE=4 ... [more]
A0A5A7VQC62.4e-12784.23Putative calcium-binding protein CML49 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1HP981.1e-12482.94probable calcium-binding protein CML49 OS=Cucurbita maxima OX=3661 GN=LOC1114660... [more]
Match NameE-valueIdentityDescription
XP_008464601.12.1e-13386.24PREDICTED: probable calcium-binding protein CML49 [Cucumis melo] >TYK04310.1 put... [more]
XP_004142653.11.8e-13286.24probable calcium-binding protein CML49 [Cucumis sativus] >KGN54506.1 hypothetica... [more]
XP_038883807.11.1e-12984.62probable calcium-binding protein CML50 [Benincasa hispida][more]
KAA0067905.15.0e-12784.23putative calcium-binding protein CML49 [Cucumis melo var. makuwa][more]
XP_022966416.12.3e-12482.94probable calcium-binding protein CML49 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G10300.32.4e-8757.27Calcium-binding EF-hand family protein [more]
AT5G04170.17.1e-8754.97Calcium-binding EF-hand family protein [more]
AT3G10300.26.2e-6753.62Calcium-binding EF-hand family protein [more]
AT3G10300.46.2e-6753.62Calcium-binding EF-hand family protein [more]
AT3G10300.51.5e-6553.44Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 124..152
e-value: 0.092
score: 21.9
coord: 190..218
e-value: 0.0097
score: 25.1
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 125..148
e-value: 5.8E-5
score: 22.4
IPR002048EF-hand domainPFAMPF13405EF-hand_6coord: 191..219
e-value: 2.2E-5
score: 23.9
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 186..221
score: 12.867577
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 120..155
score: 10.691735
NoneNo IPR availableGENE3D1.10.238.10coord: 102..290
e-value: 8.4E-40
score: 138.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 29..51
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 24..79
NoneNo IPR availablePANTHERPTHR46824:SF1CALCIUM-BINDING PROTEIN CML49-RELATEDcoord: 1..291
NoneNo IPR availableCDDcd16180EFh_PEF_Group_Icoord: 124..288
e-value: 4.7874E-76
score: 227.411
IPR044590Probable calcium-binding protein CML48/49/50PANTHERPTHR46824CALCIUM-BINDING PROTEIN CML48-RELATEDcoord: 1..291
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 133..145
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 199..211
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 117..286

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0004715.1PI0004715.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding