PI0003501 (gene) Melon (PI 482460) v1

Overview
NamePI0003501
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptiondisease resistance protein RGA2-like
Locationchr01: 25885047 .. 25887260 (-)
RNA-Seq ExpressionPI0003501
SyntenyPI0003501
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGAAGCTATTCTCCACAACGTTACTGCAGACATCATATTCAAATTGGGCTCTTCCGCACTACGAGAGCTTGGGTTGTTATGGGGTGTCAATGATGAGCTCGACAAACTCAAACACTCTCTTTCTGCCATTCAAGCCGTGCTTCTCGATGCAGAGGAGCAGCAGTCCAAGAGCTTTGCTGTCAAGGCTTGGGTTTCAAGGCTTAAGGATGTTTTGTACGAGATTGATGACCTGGTGGACGAGTCCTCTTATGAAACCTTAAGAAGGCAAGCTTTGGCCAAAGATCAGAGAAGCAGAAAACGAGTACGTATCCTCTTCTCCAAATTTAAATCTGATTGGAAAATAGATCACAAAATCAAGGATATTAGGCAGAGGCTACAATCTATTAATGATGATAAAAATCAATTTAGCTTTTCTGAGCATGTGATCGAGAAAAGAGATGACGAAGAGTTGAGAAAGAGACGGGAGACTTACTCTTACATACTTGAAGAGGAAGTGATTGGTAGGAATGATGATAAGGAAGCAGTCATAGATCTTCTATTAAATTCCAACGTCACAGAGGATATTGCAATTGTTTCCATTGTTGGAATGGGAGGACTGGGAAAGACTGCCCTTGCTCAATCTATTTATACCCATCACGATATGACTAGTAGTGAGTTCGAATTGAAGTTATGGGTGTGTGTTTCTGAAGAATTTGATCCCAAAGTTATCATCCAAAAGATGATAGAGTCTGCAACTGGGACGAAGCCGAAGTCGTTCCTTCAAATAGATTCATTACAAAGTGAGCTTAGAAAGAAAATCGATGGAAAGAAATACTTACTCGTAATGGATGATGTGTGGAATGAGAAAAAAGAGGAATGGTTACGTCTTAAAAGATTATTGATGGGTGGTGCAAAGGGTAGTAGGATTTTGATCACAACACGCAGTGTACAAGTTGCAAAAACTTTTGACACTACTTTCATCCATTCTTTACAAATTTTGGATGTGTACGATTCTTGGTTATTGTTTCAAAAAATTACTTGTTTGGAAGGACATCCAAGTAATCTAGAGGAGGTTGATCAAAATTCAAATTTGATACAAATTGGCAAGGAGATCGTGTCAAGGCTAAAAGGTGTTCCTCTCACAATAAGAACGATCGGAGGACTTTTAAAAGACAATAAATCAAAAAGATTTTGGTTGTCTTTCAAGGATAAGGAACTTTATCAGGTTTTGGGGCAAGGACAAGATAATCTAAAAGAAATGCGATTAATTCTTGAACTCAGCTATAAATACCTCCCAGCTAATTTGAAGCAATGTTTCCTATACTGTGCTTTGTTCCCCAAAGATTATGAAATTGAAAAACATGAACTTATACTACTATGGAGTGCACAAGGTTTCATTAAACCAAATGGTAACAAGGACGATAACCTCATTGATATTGGCAATGATTATTTCATGGAGTTATTATCACGATCATTTTTTCAAGAGGTTACAAAAAATAATCAGGGAGACATAATAGCATGTAAGATGCATGATTTGATGCATGATCTTGCTTGTTGGATTACAGGTAATAAATCCATGCATGGACATATCGAGAATGTCATCAACATAGGAACTTGTCACTTTTCATGGAAAGATCAATATTGTCATGAAGATCAACTTCTAAGATCATTATCAAAGGTGACAAATTTGAGGACATTTTTTATGATAGATTGTGCAAAGGATTTGAAATGGGAATTTAGAAATATCCTTCATGATCATTTGCAATTACGAGCATTGTATTTCAAATATTTGAAGAAAGCAAGGGTGGTTTTGAAGTTTACCGGTAAGTTGAAACATTTGAGATATTTGAGTATTATGGATTCATATATTTCAAATCTTCCAGATTCCATTACAAAATTGTATAATTTAGAAACATTGATCCTTCGGAATTCAAGTTTAAAAATGTTGCCTTGTGATATTGGCAATTTGATCAACCTTAAGCATTTAGATCTTTCTCATAATCAGAATTTAAAATTCCTTCCAGATTCTATTAGTGACTTGTCTAAATTGGAAGCACTCATCCTTGATGGTTGTTGGAGATTAGAAGAATTTCCAGAAGGTACAAAAAAGTTGGTCAACCTTAAGCATCTTAATATATGCAGATGTTTGAGTCTCACCTATATACCAAAAGGATTGGGCGAGCTGAGTGATCTTCAAATATTGAGATTTCAGATTAACAGAATTGCTTGA

mRNA sequence

ATGGCTGAAGCTATTCTCCACAACGTTACTGCAGACATCATATTCAAATTGGGCTCTTCCGCACTACGAGAGCTTGGGTTGTTATGGGGTGTCAATGATGAGCTCGACAAACTCAAACACTCTCTTTCTGCCATTCAAGCCGTGCTTCTCGATGCAGAGGAGCAGCAGTCCAAGAGCTTTGCTGTCAAGGCTTGGGTTTCAAGGCTTAAGGATGTTTTGTACGAGATTGATGACCTGGTGGACGAGTCCTCTTATGAAACCTTAAGAAGGCAAGCTTTGGCCAAAGATCAGAGAAGCAGAAAACGAGTACGTATCCTCTTCTCCAAATTTAAATCTGATTGGAAAATAGATCACAAAATCAAGGATATTAGGCAGAGGCTACAATCTATTAATGATGATAAAAATCAATTTAGCTTTTCTGAGCATGTGATCGAGAAAAGAGATGACGAAGAGTTGAGAAAGAGACGGGAGACTTACTCTTACATACTTGAAGAGGAAGTGATTGGACATCCAAGTAATCTAGAGGAGGTTGATCAAAATTCAAATTTGATACAAATTGGCAAGGAGATCGTGTCAAGGCTAAAAGGTGTTCCTCTCACAATAAGAACGATCGGAGGACTTTTAAAAGACAATAAATCAAAAAGATTTTGGTTGTCTTTCAAGGATAAGGAACTTTATCAGGTTTTGGGGCAAGGACAAGATAATCTAAAAGAAATGCGATTAATTCTTGAACTCAGCTATAAATACCTCCCAGCTAATTTGAAGCAATGTTTCCTATACTGTGCTTTGTTCCCCAAAGATTATGAAATTGAAAAACATGAACTTATACTACTATGGAGTGCACAAGGTTTCATTAAACCAAATGGTAACAAGGACGATAACCTCATTGATATTGGCAATGATTATTTCATGGAGTTATTATCACGATCATTTTTTCAAGAGGTTACAAAAAATAATCAGGGAGACATAATAGCATGTAAGATGCATGATTTGATGCATGATCTTGCTTGTTGGATTACAGGTAATAAATCCATGCATGGACATATCGAGAATGTCATCAACATAGGAACTTGTCACTTTTCATGGAAAGATCAATATTGTCATGAAGATCAACTTCTAAGATCATTATCAAAGGTGACAAATTTGAGGACATTTTTTATGATAGATTGTGCAAAGGATTTGAAATGGGAATTTAGAAATATCCTTCATGATCATTTGCAATTACGAGCATTGTATTTCAAATATTTGAAGAAAGCAAGGGTGGTTTTGAAGTTTACCGGTAAGTTGAAACATTTGAGATATTTGAGTATTATGGATTCATATATTTCAAATCTTCCAGATTCCATTACAAAATTGTATAATTTAGAAACATTGATCCTTCGGAATTCAAGTTTAAAAATGTTGCCTTGTGATATTGGCAATTTGATCAACCTTAAGCATTTAGATCTTTCTCATAATCAGAATTTAAAATTCCTTCCAGATTCTATTAGTGACTTGTCTAAATTGGAAGCACTCATCCTTGATGGTTGTTGGAGATTAGAAGAATTTCCAGAAGGTACAAAAAAGTTGGTCAACCTTAAGCATCTTAATATATGCAGATGTTTGAGTCTCACCTATATACCAAAAGGATTGGGCGAGCTGAGTGATCTTCAAATATTGAGATTTCAGATTAACAGAATTGCTTGA

Coding sequence (CDS)

ATGGCTGAAGCTATTCTCCACAACGTTACTGCAGACATCATATTCAAATTGGGCTCTTCCGCACTACGAGAGCTTGGGTTGTTATGGGGTGTCAATGATGAGCTCGACAAACTCAAACACTCTCTTTCTGCCATTCAAGCCGTGCTTCTCGATGCAGAGGAGCAGCAGTCCAAGAGCTTTGCTGTCAAGGCTTGGGTTTCAAGGCTTAAGGATGTTTTGTACGAGATTGATGACCTGGTGGACGAGTCCTCTTATGAAACCTTAAGAAGGCAAGCTTTGGCCAAAGATCAGAGAAGCAGAAAACGAGTACGTATCCTCTTCTCCAAATTTAAATCTGATTGGAAAATAGATCACAAAATCAAGGATATTAGGCAGAGGCTACAATCTATTAATGATGATAAAAATCAATTTAGCTTTTCTGAGCATGTGATCGAGAAAAGAGATGACGAAGAGTTGAGAAAGAGACGGGAGACTTACTCTTACATACTTGAAGAGGAAGTGATTGGACATCCAAGTAATCTAGAGGAGGTTGATCAAAATTCAAATTTGATACAAATTGGCAAGGAGATCGTGTCAAGGCTAAAAGGTGTTCCTCTCACAATAAGAACGATCGGAGGACTTTTAAAAGACAATAAATCAAAAAGATTTTGGTTGTCTTTCAAGGATAAGGAACTTTATCAGGTTTTGGGGCAAGGACAAGATAATCTAAAAGAAATGCGATTAATTCTTGAACTCAGCTATAAATACCTCCCAGCTAATTTGAAGCAATGTTTCCTATACTGTGCTTTGTTCCCCAAAGATTATGAAATTGAAAAACATGAACTTATACTACTATGGAGTGCACAAGGTTTCATTAAACCAAATGGTAACAAGGACGATAACCTCATTGATATTGGCAATGATTATTTCATGGAGTTATTATCACGATCATTTTTTCAAGAGGTTACAAAAAATAATCAGGGAGACATAATAGCATGTAAGATGCATGATTTGATGCATGATCTTGCTTGTTGGATTACAGGTAATAAATCCATGCATGGACATATCGAGAATGTCATCAACATAGGAACTTGTCACTTTTCATGGAAAGATCAATATTGTCATGAAGATCAACTTCTAAGATCATTATCAAAGGTGACAAATTTGAGGACATTTTTTATGATAGATTGTGCAAAGGATTTGAAATGGGAATTTAGAAATATCCTTCATGATCATTTGCAATTACGAGCATTGTATTTCAAATATTTGAAGAAAGCAAGGGTGGTTTTGAAGTTTACCGGTAAGTTGAAACATTTGAGATATTTGAGTATTATGGATTCATATATTTCAAATCTTCCAGATTCCATTACAAAATTGTATAATTTAGAAACATTGATCCTTCGGAATTCAAGTTTAAAAATGTTGCCTTGTGATATTGGCAATTTGATCAACCTTAAGCATTTAGATCTTTCTCATAATCAGAATTTAAAATTCCTTCCAGATTCTATTAGTGACTTGTCTAAATTGGAAGCACTCATCCTTGATGGTTGTTGGAGATTAGAAGAATTTCCAGAAGGTACAAAAAAGTTGGTCAACCTTAAGCATCTTAATATATGCAGATGTTTGAGTCTCACCTATATACCAAAAGGATTGGGCGAGCTGAGTGATCTTCAAATATTGAGATTTCAGATTAACAGAATTGCTTGA

Protein sequence

MAEAILHNVTADIIFKLGSSALRELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSFAVKAWVSRLKDVLYEIDDLVDESSYETLRRQALAKDQRSRKRVRILFSKFKSDWKIDHKIKDIRQRLQSINDDKNQFSFSEHVIEKRDDEELRKRRETYSYILEEEVIGHPSNLEEVDQNSNLIQIGKEIVSRLKGVPLTIRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQDNLKEMRLILELSYKYLPANLKQCFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDDNLIDIGNDYFMELLSRSFFQEVTKNNQGDIIACKMHDLMHDLACWITGNKSMHGHIENVINIGTCHFSWKDQYCHEDQLLRSLSKVTNLRTFFMIDCAKDLKWEFRNILHDHLQLRALYFKYLKKARVVLKFTGKLKHLRYLSIMDSYISNLPDSITKLYNLETLILRNSSLKMLPCDIGNLINLKHLDLSHNQNLKFLPDSISDLSKLEALILDGCWRLEEFPEGTKKLVNLKHLNICRCLSLTYIPKGLGELSDLQILRFQINRIA
Homology
BLAST of PI0003501 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 171.0 bits (432), Expect = 3.8e-41
Identity = 122/393 (31.04%), Postives = 186/393 (47.33%), Query Frame = 0

Query: 161 YILEEEVIGHPSNLEEVDQNSNLIQIGKEIVSRLKGVPLTIRTIGGLLKDNKSKRFWLSF 220
           ++  +   GH    EE+  N NL+ IGKEIV +  GVPL  +T+GG+L+  + +R W   
Sbjct: 318 FLFMQRAFGHQ---EEI--NPNLMAIGKEIVKKCGGVPLAAKTLGGILRFKREEREWEHV 377

Query: 221 KDKELYQVLGQGQDNLKEMRLILELSYKYLPANLKQCFLYCALFPKDYEIEKHELILLWS 280
           +D  ++  L Q + ++      L LSY +LP +L+QCF+YCA+FPKD ++ K  LI  W 
Sbjct: 378 RDSPIWN-LPQDESSILP---ALRLSYHHLPLDLRQCFVYCAVFPKDTKMAKENLIAFWM 437

Query: 281 AQGFIKPNGNKDDNLIDIGNDYFMELLSRSFFQEVTKNNQGDIIACKMHDLMHDLACWIT 340
           A GF+   GN +  L D+GN+ + EL  RSFFQE+   +       KMHDL+HDLA    
Sbjct: 438 AHGFLLSKGNLE--LEDVGNEVWNELYLRSFFQEIEVESGKTYF--KMHDLIHDLA---- 497

Query: 341 GNKSMHGHIENVINIGTCHFSWKDQYCHEDQLLRSLSKVTNLRTFFMIDCAKDLKWEFRN 400
                                          L  + +  +N+R                 
Sbjct: 498 -----------------------------TSLFSANTSSSNIR----------------- 557

Query: 401 ILHDHLQLRALYFKYLKKARVVLKFTGKLKHLRYLSIMDSYISNLPDSITKLYNLETLIL 460
                               +   + G +  + +  ++ SY    P  + K  +L  L L
Sbjct: 558 -------------------EINANYDGYMMSIGFAEVVSSY---SPSLLQKFVSLRVLNL 617

Query: 461 RNSSLKMLPCDIGNLINLKHLDLSHNQNLKFLPDSISDLSKLEALILDGCWRLEEFPEGT 520
           RNS+L  LP  IG+L++L++LDLS N  ++ LP  +  L  L+ L L  C  L   P+ T
Sbjct: 618 RNSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPKRLCKLQNLQTLDLHYCDSLSCLPKQT 624

Query: 521 KKLVNLKHLNICRCLSLTYIPKGLGELSDLQIL 554
            KL +L++L +  C SLT  P  +G L+ L+ L
Sbjct: 678 SKLGSLRNLLLDGC-SLTSTPPRIGLLTCLKSL 624


HSP 2 Score: 67.0 bits (162), Expect = 7.7e-10
Identity = 54/177 (30.51%), Postives = 91/177 (51.41%), Query Frame = 0

Query: 1   MAEAILHNVTADIIFKLGSSALRELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSF 60
           MAEA +  V    +  L S    EL LL+G  DE  +L    S IQAVL DA+E+Q    
Sbjct: 1   MAEAFIQVV----LDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDK 60

Query: 61  AVKAWVSRLKDVLYEIDDLVDESSYETLRRQALAKDQRSRKRVRILFSKFKSDWKIDHKI 120
            ++ W+ +L    YE+DD++DE  Y+T   + L + +  R   +++  + K   ++D  +
Sbjct: 61  PLENWLQKLNAATYEVDDILDE--YKTKATRFL-QSEYGRYHPKVIPFRHKVGKRMDQVM 120

Query: 121 KDIRQRLQSINDDKNQFSFSEHVIEKRDDEELRKRRETYSYILEEEVIGHPSNLEEV 178
           K    +L +I +++ +F   E +IE++        RET S + E +V G     +E+
Sbjct: 121 K----KLNAIAEERKKFHLQEKIIERQ-----AATRETGSVLTEPQVYGRDKEKDEI 161

BLAST of PI0003501 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 4.2e-40
Identity = 182/714 (25.49%), Postives = 285/714 (39.92%), Query Frame = 0

Query: 1   MAEAILHNVTADIIFKLGSSALRELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSF 60
           MAEA L  +  ++   +G     +L L++G   E +KL    S IQAVL DA+E+Q K  
Sbjct: 1   MAEAFLQVLLENLTSFIGD----KLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDK 60

Query: 61  AVKAWVSRLKDVLYEIDDLVDESSYETLRRQALAKDQRSRKRVRILF---SKFKSDWKID 120
           A++ W+ +L    YE+DD++ E   E +R +          + R+ F          KI 
Sbjct: 61  AIENWLQKLNSAAYEVDDILGECKNEAIRFE----------QSRLGFYHPGIINFRHKIG 120

Query: 121 HKIKDIRQRLQSINDDKNQFSFSEHVIEK--------------------RDDEE------ 180
            ++K+I ++L +I++++ +F F E + E+                    RD EE      
Sbjct: 121 RRMKEIMEKLDAISEERRKFHFLEKITERQAAAATRETGFVLTEPKVYGRDKEEDEIVKI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LINNVNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIK 240

Query: 241 -------------------LRKRRE-----TYSYILEE---------------------- 300
                               +K +E      Y  +L++                      
Sbjct: 241 TIIGNIERSSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTVGARG 300

Query: 301 ---------EVIG---------HPSNL---------------EEVDQNSNLIQIGKEIVS 360
                    E +G         H SNL               ++ + N NL+ IGKEIV 
Sbjct: 301 ASILATTRLEKVGSIMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEANPNLVAIGKEIVK 360

Query: 361 RLKGVPLTIRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQDNLKEMRLILELSYKYLPA 420
           +  GVPL  +T+GGLL+  + +  W   +D E++  L Q + ++      L LSY +LP 
Sbjct: 361 KCGGVPLAAKTLGGLLRFKREESEWEHVRDNEIWS-LPQDESSILP---ALRLSYHHLPL 420

Query: 421 NLKQCFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDDNLIDIGNDYFMELLSRSFF 480
           +L+QCF YCA+FPKD ++ K  LI LW A GF+   GN +  L D+GN+ + EL  RSFF
Sbjct: 421 DLRQCFAYCAVFPKDTKMIKENLITLWMAHGFLLSKGNLE--LEDVGNEVWNELYLRSFF 480

Query: 481 QEVTKNNQGDIIACKMHDLMHDLACWITGNKSMHGHIENVINIGTCHFSWKDQYCHEDQL 540
           QE+   +       K+HDL+HDLA  +    +  G+I  +                    
Sbjct: 481 QEIEAKSGNTYF--KIHDLIHDLATSLFSASASCGNIREI-------------------- 540

Query: 541 LRSLSKVTNLRTFFMIDCAKDLKWEFRNILHDHLQLRALYFKYLKKARVVLKFTGKLKHL 543
                 V + +    I  A  +     ++L   + LR L   Y K  ++     G L HL
Sbjct: 541 -----NVKDYKHTVSIGFAAVVSSYSPSLLKKFVSLRVLNLSYSKLEQLPSSI-GDLLHL 600

BLAST of PI0003501 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 167.2 bits (422), Expect = 5.5e-40
Identity = 189/710 (26.62%), Postives = 290/710 (40.85%), Query Frame = 0

Query: 1   MAEAILHNVTADIIFKLGSSALRELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSF 60
           MAEA L  +  ++ F +      ELGL++G   E  KL    S IQAVL DA+E+Q K  
Sbjct: 1   MAEAFLQVLLDNLTFFIQG----ELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYK 60

Query: 61  AVKAWVSRLKDVLYEIDDLVDESSYETLR-RQALAKDQRSRKRVRILFSKFKSDWKIDHK 120
           A+K W+ +L    YE+DD++D+   E  R +QA+      R    I F      +K+  +
Sbjct: 61  AIKNWLQKLNVAAYEVDDILDDCKTEAARFKQAVLGRYHPR---TITFC-----YKVGKR 120

Query: 121 IKDIRQRLQSINDDKNQFSFSEHVIEK------------------RDDEE---------- 180
           +K++ ++L +I +++  F   E +IE+                  R+ EE          
Sbjct: 121 MKEMMEKLDAIAEERRNFHLDERIIERQAARRQTGFVLTEPKVYGREKEEDEIVKILINN 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 VSYSEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVE 240

Query: 241 --------------LRKRRE------TYSYILEE-------------------------- 300
                         L+K+ +       Y  +L++                          
Sbjct: 241 SIEGKSLGDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLKIGASGASIL 300

Query: 301 -----EVIG---------HPSNLEEVD---------------QNSNLIQIGKEIVSRLKG 360
                E IG           SNL + D                +  L++IGKEIV +  G
Sbjct: 301 ITTRLEKIGSIMGTLQLYQLSNLSQEDCWLLFKQRAFCHQTETSPKLMEIGKEIVKKCGG 360

Query: 361 VPLTIRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQDNLKEMRLILELSYKYLPANLKQ 420
           VPL  +T+GGLL+  + +  W   +D E++  L Q ++++      L LSY +LP +L+Q
Sbjct: 361 VPLAAKTLGGLLRFKREESEWEHVRDSEIWN-LPQDENSVLP---ALRLSYHHLPLDLRQ 420

Query: 421 CFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDDNLIDIGNDYFMELLSRSFFQEVT 480
           CF YCA+FPKD +IEK  LI LW A  F+   GN +  L D+GN+ + EL  RSFFQE+ 
Sbjct: 421 CFAYCAVFPKDTKIEKEYLIALWMAHSFLLSKGNME--LEDVGNEVWNELYLRSFFQEIE 480

Query: 481 KNNQGDIIACKMHDLMHDLACWITGNKSMHGHIENVINIGTCHFSWKDQYCHEDQLLRSL 540
             +       KMHDL+HDLA  +    +    I   IN+       KD    ED +    
Sbjct: 481 VKSGKTYF--KMHDLIHDLATSMFSASASSRSIRQ-INV-------KDD---EDMMF--- 540

Query: 541 SKVTNLRTFFMIDCAKDLKWEFRNILHDHLQLRALYFKYLKKARVVLKFTGKLKHLRYLS 543
             VTN +    I  ++ +     ++    + LR L      +   +    G L HLRYL 
Sbjct: 541 -IVTNYKDMMSIGFSEVVSSYSPSLFKRFVSLRVLNLSN-SEFEQLPSSVGDLVHLRYLD 600

BLAST of PI0003501 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.5e-37
Identity = 112/337 (33.23%), Postives = 177/337 (52.52%), Query Frame = 0

Query: 180 NSNLIQIGKEIVSRLKGVPLTIRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQDNLKEM 239
           N  +  + + IV + +G+PL ++T+GG+L+       W       ++  L   + NL   
Sbjct: 356 NREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIEWERVLSSRIWD-LPADKSNLLP- 415

Query: 240 RLILELSYKYLPANLKQCFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDDNLIDIG 299
             +L +SY YLPA+LK+CF YC++FPK +  EK +++LLW A+GF++       NL ++G
Sbjct: 416 --VLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLWMAEGFLQQT-RSSKNLEELG 475

Query: 300 NDYFMELLSRSFFQEVTKNNQGDIIACKMHDLMHDLACWITG--NKSMHGHIENVINIGT 359
           N+YF EL SRS  Q+ TK          MHD +++LA + +G  +       +  ++  T
Sbjct: 476 NEYFSELESRSLLQK-TKTRY------IMHDFINELAQFASGEFSSKFEDGCKLQVSERT 535

Query: 360 CHFSW-KDQYCHEDQLLRSLSKVTNLRTFFMI-------DCAKDLKWEFRNILHDHLQLR 419
            + S+ +D Y  E     +L +V  LRTF  +        C  D +     +L    +LR
Sbjct: 536 RYLSYLRDNYA-EPMEFEALREVKFLRTFLPLSLTNSSRSCCLD-QMVSEKLLPTLTRLR 595

Query: 420 ALYFKYLKKARVVLKFTGKLKHLRYLSIMDSYISNLPDSITKLYNLETLILRN-SSLKML 479
            L   + K AR+   F   + H R+L +  + +  LP S+  +YNL+TL+L   SSLK L
Sbjct: 596 VLSLSHYKIARLPPDFFKNISHARFLDLSRTELEKLPKSLCYMYNLQTLLLSYCSSLKEL 655

Query: 480 PCDIGNLINLKHLDLSHNQNLKFLPDSISDLSKLEAL 506
           P DI NLINL++LDL     L+ +P     L  L+ L
Sbjct: 656 PTDISNLINLRYLDLI-GTKLRQMPRRFGRLKSLQTL 677


HSP 2 Score: 53.5 bits (127), Expect = 8.8e-06
Identity = 39/145 (26.90%), Postives = 76/145 (52.41%), Query Frame = 0

Query: 35  LDKLKHSLSAIQAVLLDAEEQQSKSFAVKAWVSRLKDVLYEIDDLVDESSYETLRRQALA 94
           L++L  +L  I AVL+DAEE+Q  +  V+ WV+ L+DV+Y  +D +D+ + E LR   + 
Sbjct: 39  LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN-IG 98

Query: 95  KDQRSRKRVRILFSKFKSDWKIDHKIKDIRQRLQSINDDKNQFSFSEHVIEKRDDEEL-- 154
            +  S  R+R L  +      +D   + +  RL+ +     + +   +++  ++   +  
Sbjct: 99  AESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMIP 158

Query: 155 RKRRETYSYILEEEVIGHPSNLEEV 178
           ++R  T S + E EV G   + +E+
Sbjct: 159 KQRLPTTSLVDESEVFGRDDDKDEI 182

BLAST of PI0003501 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 6.2e-36
Identity = 173/702 (24.64%), Postives = 261/702 (37.18%), Query Frame = 0

Query: 1   MAEAILHNVTADIIFKLGSSALRELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSF 60
           MAEA +      ++  L S    EL LL+G  DE  +L    S IQAVL DA+E+Q  + 
Sbjct: 1   MAEAFIQ----VLLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNK 60

Query: 61  AVKAWVSRLKDVLYEIDDLVDESSYETLRRQALAKDQRSRKRVRILFSKFKSDWKIDHKI 120
            ++ W+ +L    YE+DD++DE  Y+T +    ++ +  R   +++  + K   ++D  +
Sbjct: 61  PLENWLQKLNAATYEVDDILDE--YKT-KATRFSQSEYGRYHPKVIPFRHKVGKRMDQVM 120

Query: 121 KDIRQRLQSINDDKNQFSFSEHVIEKRDDEELRKRRETYSYILEEEVIGHPSNLEEV--- 180
           K    +L++I +++  F   E ++E++       RRET S + E +V G     +E+   
Sbjct: 121 K----KLKAIAEERKNFHLHEKIVERQ-----AVRRETGSVLTEPQVYGRDKEKDEIVKI 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LINNVSDAQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIK 240

Query: 241 -------------------------------------------DQ--------------- 300
                                                      DQ               
Sbjct: 241 AIVESIEGRPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLKVGAS 300

Query: 301 ------------------------------------------------NSNLIQIGKEIV 360
                                                           N NL+ IGKEIV
Sbjct: 301 GASVLTTTRLEKVGSIMGTLQPYELSNLSQEDCWLLFMQRAFGHQEEINPNLVAIGKEIV 360

Query: 361 SRLKGVPLTIRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQDNLKEMRLILELSYKYLP 420
            +  GVPL  +T+GG+L   + +R W   +D  ++  L Q + ++      L LSY  LP
Sbjct: 361 KKSGGVPLAAKTLGGILCFKREERAWEHVRDSPIWN-LPQDESSILP---ALRLSYHQLP 420

Query: 421 ANLKQCFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDDNLIDIGNDYFMELLSRSF 480
            +LKQCF YCA+FPKD ++EK +LI LW A GF+   GN +  L D+G++ + EL  RSF
Sbjct: 421 LDLKQCFAYCAVFPKDAKMEKEKLISLWMAHGFLLSKGNME--LEDVGDEVWKELYLRSF 480

Query: 481 FQEVTKNNQGDIIACKMHDLMHDLACWITGNKSMHGHIENVINIGTCHFSWKDQYCHEDQ 530
           FQE+   +       KMHDL+HDLA  +    +   +I                      
Sbjct: 481 FQEIEVKDGKTYF--KMHDLIHDLATSLFSANTSSSNI---------------------- 540

BLAST of PI0003501 vs. ExPASy TrEMBL
Match: A0A0A0KBD9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G420890 PE=4 SV=1)

HSP 1 Score: 969.9 bits (2506), Expect = 4.5e-279
Identity = 489/560 (87.32%), Postives = 522/560 (93.21%), Query Frame = 0

Query: 1   MAEAILHNVTADIIFKLGSSALRELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSF 60
           MAEAIL+NVTADIIFKLGSSAL+ELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKS 
Sbjct: 1   MAEAILYNVTADIIFKLGSSALQELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSL 60

Query: 61  AVKAWVSRLKDVLYEIDDLVDESSYETLRRQALAKDQRSRKRVRILFSKFKSDWKIDHKI 120
           AVKAWVSRLKD LYEIDDLVDESSYETLRRQ LAKDQR RK VRILFSKFKS+WKIDHKI
Sbjct: 61  AVKAWVSRLKDALYEIDDLVDESSYETLRRQVLAKDQRKRKLVRILFSKFKSNWKIDHKI 120

Query: 121 KDIRQRLQSINDDKNQFSFSEHVIEKRDDEELRKRRETYSYILEEEVIGHPSNLEEVDQN 180
           KDIRQRLQSINDDKNQFSFSEHVIEKRDDEELRKRRETYSYILEEEVIGHPSN E++DQ+
Sbjct: 121 KDIRQRLQSINDDKNQFSFSEHVIEKRDDEELRKRRETYSYILEEEVIGHPSNPEKLDQS 180

Query: 181 SNLIQIGKEIVSRLKGVPLTIRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQDNLKEMR 240
           S+LIQIG+EIVS+LKGVPLTIRTIGGLLKDNKSKR WLSFKD EL+++LGQGQDNLKE+R
Sbjct: 181 SSLIQIGREIVSKLKGVPLTIRTIGGLLKDNKSKRVWLSFKDNELHRILGQGQDNLKEVR 240

Query: 241 LILELSYKYLPANLKQCFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDDNLIDIGN 300
           LILELSYKYLPANLKQCFLYCALFPKDYEI+ HELIL+WSAQGFI+PNG+KD++LIDIGN
Sbjct: 241 LILELSYKYLPANLKQCFLYCALFPKDYEIKTHELILMWSAQGFIQPNGSKDNSLIDIGN 300

Query: 301 DYFMELLSRSFFQEVTKNNQGDIIACKMHDLMHDLACWITGNKSMHGHIENVINIGTCHF 360
           DYFMELLSRSFFQEVTKN +GDIIACKMHDLMHDLACWI  N+       NVINIGT HF
Sbjct: 301 DYFMELLSRSFFQEVTKNERGDIIACKMHDLMHDLACWIADNEC------NVINIGTRHF 360

Query: 361 SWKDQYCHEDQLLRSLSKVTNLRTFFMIDCAKDLKWEFRNILHDHLQLRALYFKYLKKAR 420
           SWKDQY H+DQLLRSLSKVTNLRTFFM+D A DLKWEF  ILHDHLQLRALYFK LK A 
Sbjct: 361 SWKDQYSHKDQLLRSLSKVTNLRTFFMLDSANDLKWEFTKILHDHLQLRALYFKNLKNAM 420

Query: 421 VVLKFTGKLKHLRYLSIMDSYISNLPDSITKLYNLETLILRNSSLKMLPCDIGNLINLKH 480
           +VL+FTGKLKHLRYLSIMDS+I NLPDSIT+LYNLETLILRNSS KMLP +IGNLINLKH
Sbjct: 421 IVLEFTGKLKHLRYLSIMDSFILNLPDSITELYNLETLILRNSSFKMLPDNIGNLINLKH 480

Query: 481 LDLSHNQNLKFLPDSISDLSKLEALILDGCWRLEEFPEGTKKLVNLKHLNICRCLSLTYI 540
           LDLS+N+NLKFLPDSISDL KLE LIL GC RLEEFPE TKKL+NLKHL+IC CLSLTY+
Sbjct: 481 LDLSNNRNLKFLPDSISDLCKLEELILHGCLRLEEFPEDTKKLINLKHLSICGCLSLTYL 540

Query: 541 PKGLGELSDLQILRFQINRI 561
           PK LGELSDLQILRFQINRI
Sbjct: 541 PKRLGELSDLQILRFQINRI 554

BLAST of PI0003501 vs. ExPASy TrEMBL
Match: A0A1S4E349 (putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499944 PE=4 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 2.2e-177
Identity = 384/784 (48.98%), Postives = 455/784 (58.04%), Query Frame = 0

Query: 1   MAEAILHNVTADIIFKLGSSALRELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSF 60
           MAEAIL+N TADIIF LGSSAL+E+G LW VN EL KLKHSLSAIQAVL DAEEQQSK+ 
Sbjct: 1   MAEAILYNFTADIIFTLGSSALQEIGSLWRVNSELHKLKHSLSAIQAVLHDAEEQQSKNN 60

Query: 61  AVKAWVSRLKDVLYEIDDLVDESSYETLRRQALAKDQRSRKRVRILFSKFKSDWKIDHKI 120
            VK WV +L+DVLYEIDDL+DESSY+TLRRQ LAK +R RK+VRILFSKFKS+WKI HKI
Sbjct: 61  QVKDWVLKLQDVLYEIDDLIDESSYQTLRRQVLAKHRRYRKQVRILFSKFKSNWKIGHKI 120

Query: 121 KDIRQRLQSINDDKNQFSFSEHVIEKRDDEE-LRKRRETYSYILEEEVIG---------- 180
           K+IRQRLQ+IN+DKNQFSF +HVIE+RDD+E LRKRRET+S+ILE+EVIG          
Sbjct: 121 KEIRQRLQAINEDKNQFSFRKHVIERRDDDEGLRKRRETHSFILEDEVIGRNDDKEAVID 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LLLNSNTKEDIAIVSIVGMGGLGKTALARSIYNHKRIMTRFSLKLWVCVSDAFDLEIIVQ 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 KIIESATGKKSGYLQIDSLQCELRKHIDGNKYLFVMDDVWNEKKEEWLHLKELLMAGAKG 300

Query: 301 ---------------------HPSNL------------------------EEVDQ-NSNL 360
                                HP  +                         E+DQ N NL
Sbjct: 301 SRILITTRSEQVAKTFDSTFVHPLQILDASNSWLLFQKMTGLEELSNNQEAEIDQTNLNL 360

Query: 361 IQIGKEIVSRLKGVPLTIRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQDNLKEMRLIL 420
           IQIGKEI+SRLKGVPL IRTIGGLLKDNKS+RFWLSFKDKELYQVL QGQD LKEM+ IL
Sbjct: 361 IQIGKEILSRLKGVPLVIRTIGGLLKDNKSERFWLSFKDKELYQVLRQGQDALKEMKYIL 420

Query: 421 ELSYKYLPANLKQCFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDDNLIDIGNDYF 480
           ELSYKYLPANLKQCFLYCALFPKD+E+ K  LILLW +QG+I+PNGNKD+NL+DIG+ YF
Sbjct: 421 ELSYKYLPANLKQCFLYCALFPKDFEVPKISLILLWRSQGYIQPNGNKDNNLVDIGDGYF 480

Query: 481 MELLSRSFFQEVTKNNQGDIIACKMHDLMHDLACWITGNKSMHGHIENVINIGTCHFSWK 540
           MELLSRSFFQ+V K++ GDI+ACKMHD+MHDLAC +T N+ +HG   N+IN  T HF  +
Sbjct: 481 MELLSRSFFQDVKKDDSGDIVACKMHDMMHDLACSLTNNECVHGLKGNIINKRTRHFVLE 540

Query: 541 DQYCHEDQLLRSLSKVTNLRTFF--MIDCAKDLKWEFRNILHDHLQLRALY---FKYLKK 554
           D+  HEDQL+R LSK T+LRT F    +     +W F        + R ++   +    K
Sbjct: 541 DKDYHEDQLMRPLSKATHLRTLFYRKEEDVIGSQWNFEETFRSIFRFRTVHLEGYDQNPK 600

BLAST of PI0003501 vs. ExPASy TrEMBL
Match: A0A0A0LMP4 (Rx_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G074130 PE=4 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 2.1e-164
Identity = 335/562 (59.61%), Postives = 404/562 (71.89%), Query Frame = 0

Query: 1   MAEAILHNVTADIIFKLGSSALRELG-LLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKS 60
           MAEAIL N+TADIIFKLGSSALR+ G L  GV D+ DKL HSLSAIQAVL DAEE+Q K 
Sbjct: 1   MAEAILFNLTADIIFKLGSSALRQFGSLRGGVKDDFDKLWHSLSAIQAVLHDAEEKQFKD 60

Query: 61  FAVKAWVSRLKDVLYEIDDLVDESSYETLRRQALAKDQRSRKRVRILFSKFKSDWKIDHK 120
            AV+ WVSRLKDVLYEIDDL+DE SY+ LRRQ L   Q +RK+VR LFSKF ++WKI HK
Sbjct: 61  HAVEVWVSRLKDVLYEIDDLIDEFSYQILRRQVL---QSNRKQVRTLFSKFITNWKIGHK 120

Query: 121 IKDIRQRLQSINDDKNQFSFSEHVIEKRDDEE--LRKRRETYSYILEEEVIGHPSNLEEV 180
           IK+I QRLQ+IN+DK QFSF +HVIE+RDD++  LRKRRET+S+ILE+EVIG   + E V
Sbjct: 121 IKEISQRLQNINEDKIQFSFCKHVIERRDDDDEGLRKRRETHSFILEDEVIGRNDDKEAV 180

Query: 181 DQNSNLIQIGKEIVSRLKGVPLTIRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQDNLK 240
                                     I  LL  N  +   +   +KELYQVLG+GQD LK
Sbjct: 181 --------------------------IDLLLNSNTKEDIAIVSINKELYQVLGRGQDALK 240

Query: 241 EMRLILELSYKYLP-ANLKQCFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDDN-- 300
           E++L LELSYKYLP +NLKQCFLYCALFPKDY I+K ELILLW AQGFI+ NGN DDN  
Sbjct: 241 EIQLFLELSYKYLPSSNLKQCFLYCALFPKDYRIKKDELILLWRAQGFIQQNGNNDDNSS 300

Query: 301 LIDIGNDYFMELLSRSFFQEVTKNNQGDIIACKMHDLMHDLACWITGNKSMHGHIENVIN 360
           L+DIG DYFMELLSRSFFQEV KN+ GDII CKMHDLMHDLAC IT N+ + G   NVI+
Sbjct: 301 LVDIGEDYFMELLSRSFFQEVEKNDFGDIITCKMHDLMHDLACSITNNECVRGLKGNVID 360

Query: 361 IGTCHFSWKDQYCHEDQLLRSLSKVTNLRTFFMIDCAKDLKWEFRNILHDHLQLRALYFK 420
             T H S+ ++  HEDQL+ SLSK T+LRT F+ D     +       H+  QLR L+  
Sbjct: 361 KRTHHLSF-EKVSHEDQLMGSLSKATHLRTLFIQDVCS--RCNLEETFHNIFQLRTLHLN 420

Query: 421 YLK--KARVVLKFTGKLKHLRYLSIMDSY-ISNLPDSITKLYNLETLILRNSSLKMLPCD 480
                K     KF  KLKHLRYL + +S+ ++ LPDSI +LYNLET I ++S LK LP +
Sbjct: 421 LYSPTKFAKTWKFISKLKHLRYLHLKNSFCVTYLPDSILELYNLETFIFQSSLLKKLPSN 480

Query: 481 IGNLINLKHLDLSHNQNLKFLPDSISDLSKLEALILDGCWRLEEFPEGTKKLVNLKHLNI 540
           +GNLINLKHLDLS + NL+FLPDSI+ L KLEALIL GC  L+E P+ TK+L+NLK L +
Sbjct: 481 VGNLINLKHLDLSSHLNLEFLPDSITKLYKLEALILHGCSNLKELPKYTKRLINLKSLVL 530

Query: 541 CRCLSLTYIPKGLGELSDLQIL 554
             C +LT++PKGL E+++LQ L
Sbjct: 541 YGCSALTHMPKGLSEMTNLQTL 530

BLAST of PI0003501 vs. ExPASy TrEMBL
Match: A0A5A7UYL5 (Putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G00780 PE=4 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 3.4e-162
Identity = 358/734 (48.77%), Postives = 429/734 (58.45%), Query Frame = 0

Query: 1   MAEAILHNVTADIIFKLGSSALRELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSF 60
           MAEAIL+N TADIIFKLGSSAL+ELG LW VN EL KLKHSLSAIQAVL DAEEQQSK+ 
Sbjct: 1   MAEAILYNFTADIIFKLGSSALQELGSLWRVNSELHKLKHSLSAIQAVLHDAEEQQSKNN 60

Query: 61  AVKAWVSRLKDVLYEIDDLVDESSYETLRRQALAKDQRSRKRVRILFSKFKSDWKIDHKI 120
            VK WV +L+DVLYEIDDL+DESSYETLRRQ LAK +R RK+VRILFSKFKS+WKI HKI
Sbjct: 61  QVKDWVLKLQDVLYEIDDLIDESSYETLRRQVLAKHRRYRKQVRILFSKFKSNWKIGHKI 120

Query: 121 KDIRQRLQSINDDKNQFSFSEHVIEKRDD-EELRKRRETYSYILEEEVIGHP-------- 180
           K+IR+RLQ+IN+DKNQF F +HVIE+RDD E LRKRRET+S+I ++EV+G          
Sbjct: 121 KEIRERLQAINEDKNQFGFCKHVIERRDDYEGLRKRRETHSFIPQDEVVGRNDDKEAVID 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LLLNSNTKEDIAIVSIVGMGGLGKTALAQSIYNHHSMTNDGQFQMKLWVCVSEEFDLKII 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 IQKIIESATRKKPQSFLQIDSLQCELRKKIDGKKYFLVMDDVWNEKKEEWLHLKRLLMGG 300

Query: 301 ------------------------------------------------SNLEEV--DQ-N 360
                                                           SN +EV  DQ N
Sbjct: 301 AKGSRILITTRSEQVAKTFDSTFIHLLQILDASNSWYLFQKMTGLEKCSNNQEVKHDQTN 360

Query: 361 SNLIQIGKEIVSRLKGVPLTIRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQDNLKEMR 420
           SN+IQIGK I+SRLKGVPL IRTIGGLLKDNKS+RFWLSFKDKELYQV GQGQD LKEM+
Sbjct: 361 SNMIQIGKTILSRLKGVPLVIRTIGGLLKDNKSERFWLSFKDKELYQVFGQGQDALKEMQ 420

Query: 421 LILELSYKYLPANLKQCFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDDN-LIDIG 480
           LILELSYKYLPANLKQCFLYCALFPKDY+IEK +LILLW AQGFI+ NGN DD+ LIDIG
Sbjct: 421 LILELSYKYLPANLKQCFLYCALFPKDYKIEKEKLILLWRAQGFIQANGNDDDSYLIDIG 480

Query: 481 NDYFMELLSRSFFQEVTKNNQGDIIACKMHDLMHDLACWITGNKSMHGHIENVINIGTCH 540
           +DYFMELLSRSFFQEV KN+ GDIIACKMHDLMHDLAC IT N+ + G   NVI+  T H
Sbjct: 481 DDYFMELLSRSFFQEVEKNDFGDIIACKMHDLMHDLACSITNNECVRGLKGNVIDKRTRH 540

Query: 541 FSWKDQYCHEDQLLRSLSKVTNLRTFFMIDCAKDLKWEFRNILHDHLQLRALYFKYLKKA 554
           F W+++ CHEDQL+  LSK T+LRTF    C ++    +R  L +        F+ + + 
Sbjct: 541 FLWEEE-CHEDQLMGPLSKATHLRTF---SCKENCVVGYRCNLEE-------TFRNMSRL 600

BLAST of PI0003501 vs. ExPASy TrEMBL
Match: A0A1S4E351 (putative disease resistance protein RGA4 OS=Cucumis melo OX=3656 GN=LOC103499953 PE=4 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 7.2e-160
Identity = 301/382 (78.80%), Postives = 328/382 (85.86%), Query Frame = 0

Query: 1   MAEAILHNVTADIIFKLGSSALRELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSF 60
           MAEAIL+NVTADIIFKLGSSAL+ELGLL GVNDELDKLKHSLSAIQAV+LDAE+QQSK  
Sbjct: 1   MAEAILYNVTADIIFKLGSSALQELGLLSGVNDELDKLKHSLSAIQAVVLDAEKQQSKRH 60

Query: 61  AVKAWVSRLKDVLYEIDDLVDESSYETLRRQALAKDQRSRKRVRILFSKFKSDWKIDHKI 120
           AVKAWVSRLKDVLYEIDDLVDESSYETLRRQ LAK+QR+RK VRILFSKFKS+WKIDHKI
Sbjct: 61  AVKAWVSRLKDVLYEIDDLVDESSYETLRRQVLAKEQRNRKLVRILFSKFKSNWKIDHKI 120

Query: 121 KDIRQRLQSINDDKNQFSFSEHVIEKRDDEELRKRRETYSYILEEEVIGHPSNLEEVDQ- 180
           KDIRQRLQSINDDKNQFSFSEHVIEKRDDEE RKRRETYSYILEEEVIG   + E V   
Sbjct: 121 KDIRQRLQSINDDKNQFSFSEHVIEKRDDEEFRKRRETYSYILEEEVIGRNDDKEAVLDL 180

Query: 181 --NSNLIQ-IGKEIVSRLKGVPLT--IRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQD 240
             NSN+ + I    +  + G+  T  +     + K    K FWLSFKD EL+QVLGQGQD
Sbjct: 181 LLNSNITEDIAIVSIVGMGGLGKTALLNLFIPITKTINQKEFWLSFKDNELHQVLGQGQD 240

Query: 241 NLKEMRLILELSYKYLPANLKQCFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDDN 300
           +LKE+RLILELSYKYLPA+LKQCFLYCALFPKDYEI+ H+LIL+WSAQ FI+PNGNKD+N
Sbjct: 241 DLKEVRLILELSYKYLPADLKQCFLYCALFPKDYEIKIHQLILMWSAQDFIQPNGNKDNN 300

Query: 301 LIDIGNDYFMELLSRSFFQEVTKNNQGDIIACKMHDLMHDLACWITGNKSMHGHIENVIN 360
           LIDIGNDYFMELLSRSFFQEVTKN +GDIIACKMHDLMHDLACWI  N+       NVIN
Sbjct: 301 LIDIGNDYFMELLSRSFFQEVTKNERGDIIACKMHDLMHDLACWIADNEC------NVIN 360

Query: 361 IGTCHFSWKDQYCHEDQLLRSL 377
           IGT HFSWKDQYCH+DQLLRSL
Sbjct: 361 IGTRHFSWKDQYCHKDQLLRSL 376

BLAST of PI0003501 vs. NCBI nr
Match: XP_031744540.1 (putative disease resistance protein RGA3 [Cucumis sativus])

HSP 1 Score: 879.8 bits (2272), Expect = 1.3e-251
Identity = 479/725 (66.07%), Postives = 511/725 (70.48%), Query Frame = 0

Query: 12  DIIFKLGSSALRELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSFAVKAWVSRLKD 71
           DIIFKLGSSAL+ELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKS AVKAWVSRLKD
Sbjct: 6   DIIFKLGSSALQELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSLAVKAWVSRLKD 65

Query: 72  VLYEIDDLVDESSYETLRRQALAKDQRSRKRVRILFSKFKSDWKIDHKIKDIRQRLQSIN 131
            LYEIDDLVDESSYETLRRQ LAKDQR RK VRILFSKFKS+WKIDHKIKDIRQRLQSIN
Sbjct: 66  ALYEIDDLVDESSYETLRRQVLAKDQRKRKLVRILFSKFKSNWKIDHKIKDIRQRLQSIN 125

Query: 132 DDKNQFSFSEHVIEKRDDEELRKRRETYSYILEEEVI----------------------- 191
           DDKNQFSFSEHVIEKRDDEELRKRRETYSYILEEEVI                       
Sbjct: 126 DDKNQFSFSEHVIEKRDDEELRKRRETYSYILEEEVIGRNDDKEVVIDLLLNSNITEDIA 185

Query: 192 ------------------------------------------------------------ 251
                                                                       
Sbjct: 186 IVSIVGMGGLGKTALAQSIYTHHNMTNSGFELKLWVCVSEEFDLKVIIQKMIESATGTKP 245

Query: 252 ------------------------------------------------------------ 311
                                                                       
Sbjct: 246 KPYLQIDSLQSELRKKIDGKKYLFVMDDVWNEKKEEWLRLKRLLMGGAKGSRILITTRSE 305

Query: 312 ---------------------------------GHPSNLEEVDQNSNLIQIGKEIVSRLK 371
                                            GHPSN E++DQ+S+LIQIG+EIVS+LK
Sbjct: 306 QVAKTFDSTFIHFLQILDEYNSWLLFQKITCLEGHPSNPEKLDQSSSLIQIGREIVSKLK 365

Query: 372 GVPLTIRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQDNLKEMRLILELSYKYLPANLK 431
           GVPLTIRTIGGLLKDNKSKR WLSFKD EL+++LGQGQDNLKE+RLILELSYKYLPANLK
Sbjct: 366 GVPLTIRTIGGLLKDNKSKRVWLSFKDNELHRILGQGQDNLKEVRLILELSYKYLPANLK 425

Query: 432 QCFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDDNLIDIGNDYFMELLSRSFFQEV 491
           QCFLYCALFPKDYEI+ HELIL+WSAQGFI+PNG+KD++LIDIGNDYFMELLSRSFFQEV
Sbjct: 426 QCFLYCALFPKDYEIKTHELILMWSAQGFIQPNGSKDNSLIDIGNDYFMELLSRSFFQEV 485

Query: 492 TKNNQGDIIACKMHDLMHDLACWITGNKSMHGHIENVINIGTCHFSWKDQYCHEDQLLRS 551
           TKN +GDIIACKMHDLMHDLACWI  N+       NVINIGT HFSWKDQY H+DQLLRS
Sbjct: 486 TKNERGDIIACKMHDLMHDLACWIADNEC------NVINIGTRHFSWKDQYSHKDQLLRS 545

Query: 552 LSKVTNLRTFFMIDCAKDLKWEFRNILHDHLQLRALYFKYLKKARVVLKFTGKLKHLRYL 561
           LSKVTNLRTFFM+D A DLKWEF  ILHDHLQLRALYFK LK A +VL+FTGKLKHLRYL
Sbjct: 546 LSKVTNLRTFFMLDSANDLKWEFTKILHDHLQLRALYFKNLKNAMIVLEFTGKLKHLRYL 605

BLAST of PI0003501 vs. NCBI nr
Match: KAE8646388.1 (hypothetical protein Csa_016432 [Cucumis sativus])

HSP 1 Score: 754.6 bits (1947), Expect = 6.1e-214
Identity = 430/736 (58.42%), Postives = 458/736 (62.23%), Query Frame = 0

Query: 1   MAEAILHNVTADIIFKLGSSALRELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSF 60
           MAEAIL+NVTADIIFKLGSSAL+ELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKS 
Sbjct: 1   MAEAILYNVTADIIFKLGSSALQELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSL 60

Query: 61  AVKAWVSRLKDVLYEIDDLVDESSYETLRRQALAKDQRSRKRVRILFSKFKSDWKIDHKI 120
           AVKAWVSRLKD LYEIDDLVDESSYETLRRQ LAKDQR RK VRILFSKFKS+WKIDHKI
Sbjct: 61  AVKAWVSRLKDALYEIDDLVDESSYETLRRQVLAKDQRKRKLVRILFSKFKSNWKIDHKI 120

Query: 121 KDIRQRLQSINDDKNQFSFSEHVIEKRDDEELRKRRETYSYILEEEVI------------ 180
           KDIRQRLQSINDDKNQFSFSEHVIEKRDDEELRKRRETYSYILEEEVI            
Sbjct: 121 KDIRQRLQSINDDKNQFSFSEHVIEKRDDEELRKRRETYSYILEEEVIGRNDDKEVVIDL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LLNSNITEDIAIVSIVGMGGLGKTALAQSIYTHHNMTNSGFELKLWVCVSEEFDLKVIIQ 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 KMIESATGTKPKPYLQIDSLQSELRKKIDGKKYLFVMDDVWNEKKEEWLRLKRLLMGGAK 300

Query: 301 --------------------------------------------GHPSNLEEVDQNSNLI 360
                                                       GHPSN E++DQ+S+LI
Sbjct: 301 GSRILITTRSEQVAKTFDSTFIHFLQILDEYNSWLLFQKITCLEGHPSNPEKLDQSSSLI 360

Query: 361 QIGKEIVSRLKGVPLTIRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQDNLKEMRLILE 420
           QIG+EIVS+LKGVPLTIRTIGGLLKDNKSKR WLSFKD EL+++LGQGQDNLKE+RLILE
Sbjct: 361 QIGREIVSKLKGVPLTIRTIGGLLKDNKSKRVWLSFKDNELHRILGQGQDNLKEVRLILE 420

Query: 421 LSYKYLPANLKQCFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDDNLIDIGNDYFM 480
           LSYKYLPANLKQCFLYCALFPKDYEI+ HELIL+WSAQGFI+PNG+KD++LIDIGNDYFM
Sbjct: 421 LSYKYLPANLKQCFLYCALFPKDYEIKTHELILMWSAQGFIQPNGSKDNSLIDIGNDYFM 480

Query: 481 ELLSRSFFQEVTKNNQGDIIACKMHDLMHDLACWITGNKSMHGHIENVINIGTCHFSWKD 540
           ELLSRSFFQEVTKN +GDIIACKMHDLMHDLACWI  N+       NVINIGT HFSWKD
Sbjct: 481 ELLSRSFFQEVTKNERGDIIACKMHDLMHDLACWIADNEC------NVINIGTRHFSWKD 540

Query: 541 QYCHEDQLLRSLSKVTNLRTFFMIDCAKDLKWEFRNILHDHLQLRALYFKYLKKARVVLK 561
           QY H+DQLLRSLSKVTNLRTFFM+D A DLKWEF  ILHDHLQLRALYFK LK A +VL+
Sbjct: 541 QYSHKDQLLRSLSKVTNLRTFFMLDSANDLKWEFTKILHDHLQLRALYFKNLKNAMIVLE 600

BLAST of PI0003501 vs. NCBI nr
Match: XP_016902648.1 (PREDICTED: putative disease resistance RPP13-like protein 1 isoform X1 [Cucumis melo])

HSP 1 Score: 632.1 bits (1629), Expect = 4.6e-177
Identity = 384/784 (48.98%), Postives = 455/784 (58.04%), Query Frame = 0

Query: 1   MAEAILHNVTADIIFKLGSSALRELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSF 60
           MAEAIL+N TADIIF LGSSAL+E+G LW VN EL KLKHSLSAIQAVL DAEEQQSK+ 
Sbjct: 1   MAEAILYNFTADIIFTLGSSALQEIGSLWRVNSELHKLKHSLSAIQAVLHDAEEQQSKNN 60

Query: 61  AVKAWVSRLKDVLYEIDDLVDESSYETLRRQALAKDQRSRKRVRILFSKFKSDWKIDHKI 120
            VK WV +L+DVLYEIDDL+DESSY+TLRRQ LAK +R RK+VRILFSKFKS+WKI HKI
Sbjct: 61  QVKDWVLKLQDVLYEIDDLIDESSYQTLRRQVLAKHRRYRKQVRILFSKFKSNWKIGHKI 120

Query: 121 KDIRQRLQSINDDKNQFSFSEHVIEKRDDEE-LRKRRETYSYILEEEVIG---------- 180
           K+IRQRLQ+IN+DKNQFSF +HVIE+RDD+E LRKRRET+S+ILE+EVIG          
Sbjct: 121 KEIRQRLQAINEDKNQFSFRKHVIERRDDDEGLRKRRETHSFILEDEVIGRNDDKEAVID 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LLLNSNTKEDIAIVSIVGMGGLGKTALARSIYNHKRIMTRFSLKLWVCVSDAFDLEIIVQ 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 KIIESATGKKSGYLQIDSLQCELRKHIDGNKYLFVMDDVWNEKKEEWLHLKELLMAGAKG 300

Query: 301 ---------------------HPSNL------------------------EEVDQ-NSNL 360
                                HP  +                         E+DQ N NL
Sbjct: 301 SRILITTRSEQVAKTFDSTFVHPLQILDASNSWLLFQKMTGLEELSNNQEAEIDQTNLNL 360

Query: 361 IQIGKEIVSRLKGVPLTIRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQDNLKEMRLIL 420
           IQIGKEI+SRLKGVPL IRTIGGLLKDNKS+RFWLSFKDKELYQVL QGQD LKEM+ IL
Sbjct: 361 IQIGKEILSRLKGVPLVIRTIGGLLKDNKSERFWLSFKDKELYQVLRQGQDALKEMKYIL 420

Query: 421 ELSYKYLPANLKQCFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDDNLIDIGNDYF 480
           ELSYKYLPANLKQCFLYCALFPKD+E+ K  LILLW +QG+I+PNGNKD+NL+DIG+ YF
Sbjct: 421 ELSYKYLPANLKQCFLYCALFPKDFEVPKISLILLWRSQGYIQPNGNKDNNLVDIGDGYF 480

Query: 481 MELLSRSFFQEVTKNNQGDIIACKMHDLMHDLACWITGNKSMHGHIENVINIGTCHFSWK 540
           MELLSRSFFQ+V K++ GDI+ACKMHD+MHDLAC +T N+ +HG   N+IN  T HF  +
Sbjct: 481 MELLSRSFFQDVKKDDSGDIVACKMHDMMHDLACSLTNNECVHGLKGNIINKRTRHFVLE 540

Query: 541 DQYCHEDQLLRSLSKVTNLRTFF--MIDCAKDLKWEFRNILHDHLQLRALY---FKYLKK 554
           D+  HEDQL+R LSK T+LRT F    +     +W F        + R ++   +    K
Sbjct: 541 DKDYHEDQLMRPLSKATHLRTLFYRKEEDVIGSQWNFEETFRSIFRFRTVHLEGYDQNPK 600

BLAST of PI0003501 vs. NCBI nr
Match: XP_031736581.1 (putative disease resistance protein RGA4 [Cucumis sativus] >KAE8651704.1 hypothetical protein Csa_006537 [Cucumis sativus])

HSP 1 Score: 604.0 bits (1556), Expect = 1.3e-168
Identity = 372/739 (50.34%), Postives = 442/739 (59.81%), Query Frame = 0

Query: 1   MAEAILHNVTADIIFKLGSSALRELG-LLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKS 60
           MAEAIL N+TADIIFKLGSSALR+ G L  GV D+ DKL HSLSAIQAVL DAEE+Q K 
Sbjct: 1   MAEAILFNLTADIIFKLGSSALRQFGSLRGGVKDDFDKLWHSLSAIQAVLHDAEEKQFKD 60

Query: 61  FAVKAWVSRLKDVLYEIDDLVDESSYETLRRQALAKDQRSRKRVRILFSKFKSDWKIDHK 120
            AV+ WVSRLKDVLYEIDDL+DE SY+ LRRQ L   Q +RK+VR LFSKF ++WKI HK
Sbjct: 61  HAVEVWVSRLKDVLYEIDDLIDEFSYQILRRQVL---QSNRKQVRTLFSKFITNWKIGHK 120

Query: 121 IKDIRQRLQSINDDKNQFSFSEHVIEKRDDEE--LRKRRETYSYILEEEVIG-------- 180
           IK+I QRLQ+IN+DK QFSF +HVIE+RDD++  LRKRRET+S+ILE+EVIG        
Sbjct: 121 IKEISQRLQNINEDKIQFSFCKHVIERRDDDDEGLRKRRETHSFILEDEVIGRNDDKEAV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 IDLLLNSNTKEDIAIVSIVGMPGFGKTALAQFIYNHKRIMTQFQLKIWVCVSDEFDLKIT 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 IQKIIESATGKKPKSLLQMDPLQCELRKQIDGKKYLIVMDDVWNEKKEKWLHLKRLLMGG 300

Query: 301 -----------------------------------------------HPSNLE-EVDQ-N 360
                                                          H +N E E+DQ N
Sbjct: 301 AKGSRILITTRSEQVAKTFDSTFVHLLQILDASNSWLLFQKMIGLEEHSNNQEIELDQKN 360

Query: 361 SNLIQIGKEIVSRLKGVPLTIRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQDNLKEMR 420
           SNLIQIG EIVS L+GVPL IRTIGGLLKDNKS+RFWLSFK+KELYQVLG+GQD LKE++
Sbjct: 361 SNLIQIGMEIVSTLRGVPLLIRTIGGLLKDNKSERFWLSFKNKELYQVLGRGQDALKEIQ 420

Query: 421 LILELSYKYLP-ANLKQCFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDDN--LID 480
           L LELSYKYLP +NLKQCFLYCALFPKDY I+K ELILLW AQGFI+ NGN DDN  L+D
Sbjct: 421 LFLELSYKYLPSSNLKQCFLYCALFPKDYRIKKDELILLWRAQGFIQQNGNNDDNSSLVD 480

Query: 481 IGNDYFMELLSRSFFQEVTKNNQGDIIACKMHDLMHDLACWITGNKSMHGHIENVINIGT 540
           IG DYFMELLSRSFFQEV KN+ GDII CKMHDLMHDLAC IT N+ + G   NVI+  T
Sbjct: 481 IGEDYFMELLSRSFFQEVEKNDFGDIITCKMHDLMHDLACSITNNECVRGLKGNVIDKRT 540

Query: 541 CHFSWKDQYCHEDQLLRSLSKVTNLRTFFMIDCAKDLKWEFRNILHDHLQLRALYFKYLK 554
            H S+ ++  HEDQL+ SLSK T+LRT F+ D     +       H+  QLR L+     
Sbjct: 541 HHLSF-EKVSHEDQLMGSLSKATHLRTLFIQDVCS--RCNLEETFHNIFQLRTLHLNLYS 600

BLAST of PI0003501 vs. NCBI nr
Match: KAA0058609.1 (putative disease resistance RPP13-like protein 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 581.6 bits (1498), Expect = 7.1e-162
Identity = 358/734 (48.77%), Postives = 429/734 (58.45%), Query Frame = 0

Query: 1   MAEAILHNVTADIIFKLGSSALRELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSF 60
           MAEAIL+N TADIIFKLGSSAL+ELG LW VN EL KLKHSLSAIQAVL DAEEQQSK+ 
Sbjct: 1   MAEAILYNFTADIIFKLGSSALQELGSLWRVNSELHKLKHSLSAIQAVLHDAEEQQSKNN 60

Query: 61  AVKAWVSRLKDVLYEIDDLVDESSYETLRRQALAKDQRSRKRVRILFSKFKSDWKIDHKI 120
            VK WV +L+DVLYEIDDL+DESSYETLRRQ LAK +R RK+VRILFSKFKS+WKI HKI
Sbjct: 61  QVKDWVLKLQDVLYEIDDLIDESSYETLRRQVLAKHRRYRKQVRILFSKFKSNWKIGHKI 120

Query: 121 KDIRQRLQSINDDKNQFSFSEHVIEKRDD-EELRKRRETYSYILEEEVIGHP-------- 180
           K+IR+RLQ+IN+DKNQF F +HVIE+RDD E LRKRRET+S+I ++EV+G          
Sbjct: 121 KEIRERLQAINEDKNQFGFCKHVIERRDDYEGLRKRRETHSFIPQDEVVGRNDDKEAVID 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 LLLNSNTKEDIAIVSIVGMGGLGKTALAQSIYNHHSMTNDGQFQMKLWVCVSEEFDLKII 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 IQKIIESATRKKPQSFLQIDSLQCELRKKIDGKKYFLVMDDVWNEKKEEWLHLKRLLMGG 300

Query: 301 ------------------------------------------------SNLEEV--DQ-N 360
                                                           SN +EV  DQ N
Sbjct: 301 AKGSRILITTRSEQVAKTFDSTFIHLLQILDASNSWYLFQKMTGLEKCSNNQEVKHDQTN 360

Query: 361 SNLIQIGKEIVSRLKGVPLTIRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQDNLKEMR 420
           SN+IQIGK I+SRLKGVPL IRTIGGLLKDNKS+RFWLSFKDKELYQV GQGQD LKEM+
Sbjct: 361 SNMIQIGKTILSRLKGVPLVIRTIGGLLKDNKSERFWLSFKDKELYQVFGQGQDALKEMQ 420

Query: 421 LILELSYKYLPANLKQCFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDDN-LIDIG 480
           LILELSYKYLPANLKQCFLYCALFPKDY+IEK +LILLW AQGFI+ NGN DD+ LIDIG
Sbjct: 421 LILELSYKYLPANLKQCFLYCALFPKDYKIEKEKLILLWRAQGFIQANGNDDDSYLIDIG 480

Query: 481 NDYFMELLSRSFFQEVTKNNQGDIIACKMHDLMHDLACWITGNKSMHGHIENVINIGTCH 540
           +DYFMELLSRSFFQEV KN+ GDIIACKMHDLMHDLAC IT N+ + G   NVI+  T H
Sbjct: 481 DDYFMELLSRSFFQEVEKNDFGDIIACKMHDLMHDLACSITNNECVRGLKGNVIDKRTRH 540

Query: 541 FSWKDQYCHEDQLLRSLSKVTNLRTFFMIDCAKDLKWEFRNILHDHLQLRALYFKYLKKA 554
           F W+++ CHEDQL+  LSK T+LRTF    C ++    +R  L +        F+ + + 
Sbjct: 541 FLWEEE-CHEDQLMGPLSKATHLRTF---SCKENCVVGYRCNLEE-------TFRNMSRL 600

BLAST of PI0003501 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 159.1 bits (401), Expect = 1.1e-38
Identity = 112/337 (33.23%), Postives = 177/337 (52.52%), Query Frame = 0

Query: 180 NSNLIQIGKEIVSRLKGVPLTIRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQDNLKEM 239
           N  +  + + IV + +G+PL ++T+GG+L+       W       ++  L   + NL   
Sbjct: 356 NREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVIEWERVLSSRIWD-LPADKSNLLP- 415

Query: 240 RLILELSYKYLPANLKQCFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDDNLIDIG 299
             +L +SY YLPA+LK+CF YC++FPK +  EK +++LLW A+GF++       NL ++G
Sbjct: 416 --VLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLWMAEGFLQQT-RSSKNLEELG 475

Query: 300 NDYFMELLSRSFFQEVTKNNQGDIIACKMHDLMHDLACWITG--NKSMHGHIENVINIGT 359
           N+YF EL SRS  Q+ TK          MHD +++LA + +G  +       +  ++  T
Sbjct: 476 NEYFSELESRSLLQK-TKTRY------IMHDFINELAQFASGEFSSKFEDGCKLQVSERT 535

Query: 360 CHFSW-KDQYCHEDQLLRSLSKVTNLRTFFMI-------DCAKDLKWEFRNILHDHLQLR 419
            + S+ +D Y  E     +L +V  LRTF  +        C  D +     +L    +LR
Sbjct: 536 RYLSYLRDNYA-EPMEFEALREVKFLRTFLPLSLTNSSRSCCLD-QMVSEKLLPTLTRLR 595

Query: 420 ALYFKYLKKARVVLKFTGKLKHLRYLSIMDSYISNLPDSITKLYNLETLILRN-SSLKML 479
            L   + K AR+   F   + H R+L +  + +  LP S+  +YNL+TL+L   SSLK L
Sbjct: 596 VLSLSHYKIARLPPDFFKNISHARFLDLSRTELEKLPKSLCYMYNLQTLLLSYCSSLKEL 655

Query: 480 PCDIGNLINLKHLDLSHNQNLKFLPDSISDLSKLEAL 506
           P DI NLINL++LDL     L+ +P     L  L+ L
Sbjct: 656 PTDISNLINLRYLDLI-GTKLRQMPRRFGRLKSLQTL 677


HSP 2 Score: 53.5 bits (127), Expect = 6.3e-07
Identity = 39/145 (26.90%), Postives = 76/145 (52.41%), Query Frame = 0

Query: 35  LDKLKHSLSAIQAVLLDAEEQQSKSFAVKAWVSRLKDVLYEIDDLVDESSYETLRRQALA 94
           L++L  +L  I AVL+DAEE+Q  +  V+ WV+ L+DV+Y  +D +D+ + E LR   + 
Sbjct: 39  LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN-IG 98

Query: 95  KDQRSRKRVRILFSKFKSDWKIDHKIKDIRQRLQSINDDKNQFSFSEHVIEKRDDEEL-- 154
            +  S  R+R L  +      +D   + +  RL+ +     + +   +++  ++   +  
Sbjct: 99  AESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAMIP 158

Query: 155 RKRRETYSYILEEEVIGHPSNLEEV 178
           ++R  T S + E EV G   + +E+
Sbjct: 159 KQRLPTTSLVDESEVFGRDDDKDEI 182

BLAST of PI0003501 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 153.3 bits (386), Expect = 5.8e-37
Identity = 125/378 (33.07%), Postives = 180/378 (47.62%), Query Frame = 0

Query: 180 NSNLIQIGKEIVSRLKGVPLTIRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQDNLKEM 239
           N  L  IGK I  + KG+PL  R I   L+   +   W        Y V          +
Sbjct: 352 NQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDW--------YAVSKNFSSYTNSI 411

Query: 240 RLILELSYKYLPANLKQCFLYCALFPKDYEIEKHELILLWSAQGFI-KPNGNKDDNLIDI 299
             +L+LSY  LP  LK+CF  C++FPK +  ++ EL+LLW A   + +P  ++   L DI
Sbjct: 412 LPVLKLSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLWMAIDLLYQPRSSR--RLEDI 471

Query: 300 GNDYFMELLSRSFFQEVTKNNQGDIIACKMHDLMHDLACWITGNKSMHGHIENVINI--G 359
           GNDY  +L+++SFFQ +       + +  MHDLM+DLA  ++G+       +N+  I   
Sbjct: 472 GNDYLGDLVAQSFFQRLDIT----MTSFVMHDLMNDLAKAVSGDFCFRLEDDNIPEIPST 531

Query: 360 TCHFSWKDQYCHEDQLLRSLSKVTNLRTFFMIDCAKDLKWEFRNILHDHLQLRALYFKYL 419
           T HFS+    C      RS+     LRT    +    L         + LQL        
Sbjct: 532 TRHFSFSRSQCDASVAFRSICGAEFLRTILPFNSPTSL---------ESLQL-------- 591

Query: 420 KKARVVLKFTGKLKHLRYLSIMDSYISNLPDSITKLYNLETLILRNSSLKMLPCDIGNLI 479
              +V+      L  LR LS+    I+NLP S+  L  L  L L ++ +K LP  +  L 
Sbjct: 592 -TEKVLNPLLNALSGLRILSLSHYQITNLPKSLKGLKLLRYLDLSSTKIKELPEFVCTLC 651

Query: 480 NLKHLDLSHNQNLKFLPDSISDLSKLEALILDGCWRLEEFPEGTKKLVNLKHLNICRCLS 539
           NL+ L LS+ ++L  LP SI++L  L  L L G   L E P G KKL +L+ L+      
Sbjct: 652 NLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGT-PLVEMPPGIKKLRSLQKLSNFVIGR 694

Query: 540 LTYIPKGLGELSDLQILR 555
           L+    GL EL +L  LR
Sbjct: 712 LS--GAGLHELKELSHLR 694


HSP 2 Score: 43.1 bits (100), Expect = 8.5e-04
Identity = 21/61 (34.43%), Postives = 37/61 (60.66%), Query Frame = 0

Query: 35 LDKLKHSLSAIQAVLLDAEEQQSKSFAVKAWVSRLKDVLYEIDDLVDESSYETLRRQALA 94
          L +LK +L     VL DA+++      VK W++ +KD  ++ +D++DE   E LRR+ +A
Sbjct: 36 LKRLKVALVTANPVLADADQRAEHVREVKHWLTGIKDAFFQAEDILDELQTEALRRRVVA 95

Query: 95 K 96
          +
Sbjct: 96 E 96

BLAST of PI0003501 vs. TAIR 10
Match: AT3G07040.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 139.8 bits (351), Expect = 6.6e-33
Identity = 122/447 (27.29%), Postives = 202/447 (45.19%), Query Frame = 0

Query: 126 RLQSINDDKNQFSFSEHVIEKRDDEELRKRRETYSYILEEEVIGHPSNLEEVDQNSNLIQ 185
           R+     D N  SF   +   + + EL K  E +     +     P++LE+  +  NL  
Sbjct: 309 RVMMTTRDMNVASFPYGIGSTKHEIELLKEDEAWVLFSNK---AFPASLEQC-RTQNLEP 368

Query: 186 IGKEIVSRLKGVPLTIRTIGGLLKDNKSKRFWLSFKDKELYQVLGQGQDN---LKEMRLI 245
           I +++V R +G+PL I ++G ++   K +  W     K++Y  L    +N   LK +R I
Sbjct: 369 IARKLVERCQGLPLAIASLGSMMSTKKFESEW-----KKVYSTLNWELNNNHELKIVRSI 428

Query: 246 LELSYKYLPANLKQCFLYCALFPKDYEIEKHELILLWSAQGFIKP-NGNKDDNLIDIGND 305
           + LS+  LP  LK+CFLYC+LFP +Y +++  LI +W AQ F++P  G K +   ++ + 
Sbjct: 429 MFLSFNDLPYPLKRCFLYCSLFPVNYRMKRKRLIRMWMAQRFVEPIRGVKAE---EVADS 488

Query: 306 YFMELLSRSFFQEVTKNNQGDIIACKMHDLMHDLACWITG--------NKSMHGH--IEN 365
           Y  EL+ R+  Q +  N  G   A KMHD++ ++A  ++         N    G    E 
Sbjct: 489 YLNELVYRNMLQVILWNPFGRPKAFKMHDVIWEIALSVSKLERFCDVYNDDSDGDDAAET 548

Query: 366 VINIGTCHFSWKDQYCHEDQLLRSLSKVTNLRTFFMIDCAKDLKWEFRNILHDHLQLRAL 425
           + N G+ H       C + ++     + TNL +  +   AK                   
Sbjct: 549 MENYGSRHL------CIQKEMTPDSIRATNLHSLLVCSSAKH------------------ 608

Query: 426 YFKYLKKARVVLKFTGKLKHLRYLSIMDSYISNLPDSITKLYNLETLILRNSSLKMLPCD 485
                      ++    L  LR L + DS IS LPD +  ++NL+ L L  + +K LP +
Sbjct: 609 ----------KMELLPSLNLLRALDLEDSSISKLPDCLVTMFNLKYLNLSKTQVKELPKN 668

Query: 486 IGNLINLKHLDLSHNQNLKFLPDSISDLSKLEALIL-------DGCWRLEEFPEGTKKLV 545
              L+NL+ L+  H++ ++ LP  +  L KL  LI        D  W          K+ 
Sbjct: 669 FHKLVNLETLNTKHSK-IEELPLGMWKLKKLRYLITFRRNEGHDSNWNYVLGTRVVPKIW 708

Query: 546 NLKHLNICRCLSL-TYIPKGLGELSDL 551
            LK L +  C +    + K LG ++ L
Sbjct: 729 QLKDLQVMDCFNAEDELIKNLGCMTQL 708

BLAST of PI0003501 vs. TAIR 10
Match: AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 126.3 bits (316), Expect = 7.6e-29
Identity = 109/357 (30.53%), Postives = 165/357 (46.22%), Query Frame = 0

Query: 179 QNSNLIQIGKEIVSRLKGVPLTIRTIGGLL--KD---NKSKRFWLSFKDKELYQVLGQGQ 238
           +   L  +GKEIV++ KG+PLTI+ +GGLL  KD   ++ +R    F+D+     L    
Sbjct: 340 ERPELEDVGKEIVTKCKGLPLTIKAVGGLLLCKDHVYHEWRRIAEHFQDE-----LRGNT 399

Query: 239 DNLKEMRLILELSYKYLPANLKQCFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDD 298
                +   L+LSY  LP++LK C L  +L+P+D  I K +L+  W  +GF+     +  
Sbjct: 400 SETDNVMSSLQLSYDELPSHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFVMWRNGR-- 459

Query: 299 NLIDIGNDYFMELLSRSFFQEVTKNNQGDIIACKMHDLMHDLACWITGNKSMHGHIENVI 358
           +  + G D F  L +R   + V K   G II CK+HD++ DL   I    S      N  
Sbjct: 460 SATESGEDCFSGLTNRCLIEVVDKTYSGTIITCKIHDMVRDLVIDIAKKDS----FSNPE 519

Query: 359 NIGTCHFSWKDQYCHEDQLLRSLSKVTNLRTFFMIDCAKDLKWEFRNILHDHLQLRALYF 418
            +   H      +  +++ ++   K+  +            K    N L+  L  +    
Sbjct: 520 GLNCRHLGISGNF--DEKQIKVNHKLRGV--------VSTTKTGEVNKLNSDLAKKFTDC 579

Query: 419 KYLKKARVVLKFTGKLKHLRYLSIMDSYISNLPDSITKLYNLETLILRNS-SLKMLPCDI 478
           KYL+    VL  +         SI D+ +S + D I  L +L  L L N+  L   P  +
Sbjct: 580 KYLR----VLDIS--------KSIFDAPLSEILDEIASLQHLACLSLSNTHPLIQFPRSM 639

Query: 479 GNLINLKHLDLSHNQNLKFLPDSISDLSKLEALILDGCWRLEEFPEGTKKLVNLKHL 530
            +L NL+ LD S+ QNLK L   I    KL  L +  C  LE FP+G   LV L+ L
Sbjct: 640 EDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKLEVL 663

BLAST of PI0003501 vs. TAIR 10
Match: AT3G50950.1 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 126.3 bits (316), Expect = 7.6e-29
Identity = 109/357 (30.53%), Postives = 165/357 (46.22%), Query Frame = 0

Query: 179 QNSNLIQIGKEIVSRLKGVPLTIRTIGGLL--KD---NKSKRFWLSFKDKELYQVLGQGQ 238
           +   L  +GKEIV++ KG+PLTI+ +GGLL  KD   ++ +R    F+D+     L    
Sbjct: 340 ERPELEDVGKEIVTKCKGLPLTIKAVGGLLLCKDHVYHEWRRIAEHFQDE-----LRGNT 399

Query: 239 DNLKEMRLILELSYKYLPANLKQCFLYCALFPKDYEIEKHELILLWSAQGFIKPNGNKDD 298
                +   L+LSY  LP++LK C L  +L+P+D  I K +L+  W  +GF+     +  
Sbjct: 400 SETDNVMSSLQLSYDELPSHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFVMWRNGR-- 459

Query: 299 NLIDIGNDYFMELLSRSFFQEVTKNNQGDIIACKMHDLMHDLACWITGNKSMHGHIENVI 358
           +  + G D F  L +R   + V K   G II CK+HD++ DL   I    S      N  
Sbjct: 460 SATESGEDCFSGLTNRCLIEVVDKTYSGTIITCKIHDMVRDLVIDIAKKDS----FSNPE 519

Query: 359 NIGTCHFSWKDQYCHEDQLLRSLSKVTNLRTFFMIDCAKDLKWEFRNILHDHLQLRALYF 418
            +   H      +  +++ ++   K+  +            K    N L+  L  +    
Sbjct: 520 GLNCRHLGISGNF--DEKQIKVNHKLRGV--------VSTTKTGEVNKLNSDLAKKFTDC 579

Query: 419 KYLKKARVVLKFTGKLKHLRYLSIMDSYISNLPDSITKLYNLETLILRNS-SLKMLPCDI 478
           KYL+    VL  +         SI D+ +S + D I  L +L  L L N+  L   P  +
Sbjct: 580 KYLR----VLDIS--------KSIFDAPLSEILDEIASLQHLACLSLSNTHPLIQFPRSM 639

Query: 479 GNLINLKHLDLSHNQNLKFLPDSISDLSKLEALILDGCWRLEEFPEGTKKLVNLKHL 530
            +L NL+ LD S+ QNLK L   I    KL  L +  C  LE FP+G   LV L+ L
Sbjct: 640 EDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKLEVL 663

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7XA423.8e-4131.04Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XA394.2e-4025.49Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q7XA405.5e-4026.62Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q9LRR41.5e-3733.23Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702... [more]
Q7XBQ96.2e-3624.64Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KBD94.5e-27987.32Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G420890 PE=4 SV=1[more]
A0A1S4E3492.2e-17748.98putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo OX=3... [more]
A0A0A0LMP42.1e-16459.61Rx_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G074130 PE=4 S... [more]
A0A5A7UYL53.4e-16248.77Putative disease resistance RPP13-like protein 1 isoform X1 OS=Cucumis melo var.... [more]
A0A1S4E3517.2e-16078.80putative disease resistance protein RGA4 OS=Cucumis melo OX=3656 GN=LOC103499953... [more]
Match NameE-valueIdentityDescription
XP_031744540.11.3e-25166.07putative disease resistance protein RGA3 [Cucumis sativus][more]
KAE8646388.16.1e-21458.42hypothetical protein Csa_016432 [Cucumis sativus][more]
XP_016902648.14.6e-17748.98PREDICTED: putative disease resistance RPP13-like protein 1 isoform X1 [Cucumis ... [more]
XP_031736581.11.3e-16850.34putative disease resistance protein RGA4 [Cucumis sativus] >KAE8651704.1 hypothe... [more]
KAA0058609.17.1e-16248.77putative disease resistance RPP13-like protein 1 isoform X1 [Cucumis melo var. m... [more]
Match NameE-valueIdentityDescription
AT3G14470.11.1e-3833.23NB-ARC domain-containing disease resistance protein [more]
AT3G14460.15.8e-3733.07LRR and NB-ARC domains-containing disease resistance protein [more]
AT3G07040.16.6e-3327.29NB-ARC domain-containing disease resistance protein [more]
AT3G50950.27.6e-2930.53HOPZ-ACTIVATED RESISTANCE 1 [more]
AT3G50950.17.6e-2930.53HOPZ-ACTIVATED RESISTANCE 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 35..55
NoneNo IPR availableGENE3D1.20.5.4130coord: 12..121
e-value: 4.5E-21
score: 77.0
NoneNo IPR availablePANTHERPTHR23155:SF1132OS08G0543100 PROTEINcoord: 32..144
coord: 180..532
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 327..560
IPR042197Apoptotic protease-activating factors, helical domainGENE3D1.10.8.430coord: 171..246
e-value: 2.7E-7
score: 32.1
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 247..337
e-value: 3.2E-19
score: 70.8
IPR001611Leucine-rich repeatPFAMPF13855LRR_8coord: 454..510
e-value: 1.3E-7
score: 31.3
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 477..499
score: 7.041994
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 342..561
e-value: 5.7E-33
score: 115.6
IPR041118Rx, N-terminalPFAMPF18052Rx_Ncoord: 16..97
e-value: 4.7E-22
score: 78.1
IPR044974Disease resistance protein, plantsPANTHERPTHR23155DISEASE RESISTANCE PROTEIN RPcoord: 32..144
coord: 180..532
IPR038005Virus X resistance protein-like, coiled-coil domainCDDcd14798RX-CC_likecoord: 16..130
e-value: 9.47212E-16
score: 71.8843
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 176..261

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0003501.1PI0003501.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0005515 protein binding