PI0003412 (gene) Melon (PI 482460) v1

Overview
NamePI0003412
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptiontranscription factor Pur-alpha 1
Locationchr02: 2957572 .. 2962896 (-)
RNA-Seq ExpressionPI0003412
SyntenyPI0003412
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCGAAATATGTTTCTTCTTCTTCATCATCTTTCATTGTCATTTTAATTTCAATTTCCAACTGTTAAAAACCCCATCACAAATCGTTCATGTAATTTCTAACCCTAATTTTCCAGAACCCTTTTGTCAAAATCCTGTTTTTTTTTTTTTTTTTTCTCTTTTTCTCTCATGGGGTTCACTTATTAGATCTCCCATTTCTAGGTTTCTGATTCTTTTCTTGTATTTTGTTTGCATTCAAGGCATTTGGCGGGTTTTTGCATCGATTTCTGTTCGAATCGGGGCTTGTTCTTTTTAATTTATGTGTAATTTTGTCTTTTTTGGTAAACCCAATTGGAGAAAAAATGATGCTGATTTATTGAATTGAGATTCTTGTACGAATTTAGATAATCGCTTAAATCGTGGGGAATTTTAAGTAGAAACCGAGTCAGAGCTGGTAGTGGGAGATGGAGGGGAATTCCGGCGGAGGTGGAGTGGGAATTGGAGGAACGACGACGGGTGGCGTGGCGGCGGGAGGAGGGGCTGGTGGCGGCGGAGGGAATGACGTGGAGTTGATGTGCAAAACACTGCAGGTGGAACACAAGCTGTTTTACTTCGATCTGAAGGAGAATCCCAGAGGTCGATATCTGAAGATTTCAGAGAAAACATCGGCTACAAGGTCAACGATCATTGTTCCTTTCTCTGGGATTCCATGGTTCTTAGATCTCTTCAATTACTATATCAATTCTGATGATCCGGAGGTTTTTAGCAAGGAATTGCAGCTGGACACAAAGGCATCTTTGTTTCTCTTCTTTCTCTTTTGCCTTTTAGTTTATCTCTTTGTTCTTGTTGGTCAACTTTTGTCTTTCAGGTGTTTTACTTTGACATTGGCGAGAATAGGAGAGGTCGTTTCTTGAAGGTAAATTGCTTTTCCTGCTACCCCTTTTGTTGTTTGTTTCTTTTCTCGAGATTCCAACTTTCTGGAATATCCATTTTTTCCTTGAAAGTACTCTCTGCAGTTTGTTTGTTTGATTCTGCCAAATTAGTGTTGGATGGATCTGCATAGATTTGAGAATGGGATTGTTCTTGTTGGTCTAGACTTTAGAGTTTCGCTCAAAATTTGCACAGTGCTTTAGAAAAAGCTGACATATCCTCATGTCTTTTCCCTTGATGATTTTTTTCTGAAATTTTTCTTTGGCTGAAGTTCTTCTTCTGCCAAGTTCTTTCAGCTTCTGGTGCCCAAGAAATCAACTCTTATCAGAGAGAGAAGAAAAGTAATGAAATTACTTGAACTTTGAGTTTGTTATCCAGTCATATTCCATGTCCCGTGTCCTTTTAAAGAAAAAGACCACTCTTTATTTTCTTGATGACTTTTATAGCTTCTATTTGACAATACTTCCAAATGCATCTTTCCAAATATGAATTATACTATGATTTATGGAGATTCCTTAACAGTTTGACTTGACCTTTTCTTGTTCTTTACTTTTTTCTTTTTCTTGTTTTTTTTTTCCTTAAAAAATGTTTTTCATTCATCTTTCTCACTCACTTATTCATCTGTCATAGTATGAAGACTTATGACAAGCCAAAGGTTCTTTGCAAAATAATTAGGTTTATGAAACCGGAAGCCTAGTAAAAATAAAAAGACAAGAATATGTTGGCACATGACTTTTCTAATCATTAGCATGGACATGTGAAGGACATAGAATGTAGAAAATGCCTTCTTAGTGTATATATTTTAATATAATATAGGAATAATTGCAAAAACACCCCAGAGTATGGTGGTAGTTGCAATTATACCCTTAAACTTTCAAAATAAGAAATTGAGCTCTCAAAGTTATCCAAATTGTAAAATTTGATCCTCAAACTTACATAATTGTTTTGATTATATAAATTTGAGGATTCAATTCTTATCATTTTTGGGTTTAATTTTTACAATTATTGTAAGCTTGAGGGTCCAATTTCAACACTTTTAGAAGTTTAGGGGTTCGATTGTTATTATTGAAAGTTTGAGGGTGTAATTGCAACTACCACCATATTTTAGGGATGGTTTTTGCAATTTGCCCTATAATATACAATGATATTAAAGCAATTGGGCAATTTAAAAAAAAAAAGTTTTGTGGAAATTGATACCCAAAACACTTTCTAAACGACGTGTTACCTTACAAAACAGAGGTTCATGTTAAAGTGAAACTCAAGTATACATGTAATAATATGTTTCTATAATGACTCCACATAGGCTAGAAGGTTCCATTTCAAACATTATTTACTGAATTCTCCAGATTTTTTCATCTGTGACAACTTAAAAAAGGTTCTCCATCTAGTTTTACTTCACTTAATAGCCTTCTTTAATTTTCTCATTCTAATTTTTGTGTTTCCCGTCTGGATGTTGGATGCATTAAAGGTTATAGATGTAGATATATTACCCAAGAAGTGTGTCATCCATGGTTCATATGCTTCCTCTGGAGCCAACTTTGTGCCACTAATACATTATAAATTATTCACAGTCATGTGTGGCTCTCTGCAACTTGATTTAAGCTAGTAGTCTAGTGCGCCTTCTCCTTGATTATTTGGTTTCAGATTGGATGATGTACATCAGGCATTAGCGTAGTCTTGAGCGAAGTGGTTTGAAGGCAAACTTTAGAACTTGCCAACTAGGAAATTGCATAAATGGAGGTTGAAGCCTCCTTGTTTAGCTTTTCATTCTGAATCTGATGATAAATCTATTTTCCACTCCAGTCGTTAATTATTTTCTGTATGGAGCATGTGAGCATTTCCTGCACACTTTAAGTTCTCCCTCTTGCTCATAACTCTCTAATTTTTTATTATAACAATTGTTCTTGGAATGGATTACTGGAACACAGTAATGTGACTAGACACTGAAACTAAGGACATGGCTGACACCTCATTTGGCCCTAGTTATCAAAAGCATTTTTCAATTCTGAGGATCAGACCTTGAAACTGGACGTGCTAATAAGTTAGGGCAAGGCTTGTATTGACTATTGAGTGTTGGAAGTGAATCTGTCTAGTGAAATAAAATGCATGTTTAGCTGTTCTTAGGGTTCTTGTGATAGGTGTTCTCCAGGAAAATTATAAAGAGAGAAGGAAATGCTGAAGTAGAATAGTTTATTCTCTTTATGTCTGCTGTAACCAGCTTTATAGAAGTTCCATTTTTTCCATCACCGTCTTCAGCCAGCCATTACCACTTGCTTCCCTGTCAAGGAGTTGACTTACTGTGCAAAATAACCCACACAGGATGCCATTGTGTGGCAGTAGATTACAACCACCTCTGGCTCCATTACTCCTAAATACCAGGCTGCCATTGTGTGTCAGTTCATGTAAGCTGAATCTGCAGTTCACTTATTCCCAGTCTACTCAAGTTCTGTCCCTTGACTATTGCTTGTTGAATCTACAATAAGCTTTCCATGTGTACTACAGATCTCCAAGGCTCCGATGTTTCTTTCTGCTTTAGCTTTTTAATTTGTTTGAAGTGTCTTTAATCATAGATGTGAACGTGTTGTGTAGTTTTATGAGTATAAATCAACTCTCTGTATTTTATATGATGTTTATAAATGGCTGCATATTATCATCAAGTTTGTAACTGATTCCATTTTTTTTCTTTTAAATACCAGTGTGGATCTGAACCGTCAACCATTGACTACTTTGAGCTTATATCGGCTCTCATTTATTCTTATTCGTTCCCAACTTAAATTCTGTTAAAGTAAATACCATTAATTCTGGGATTTGAGGGTTGGTAATTTGACCTCTTCAGCTATAGACACTATTGTGTGTACTGTTGCCATCTTCTCGATTGTAAGGCTTAAGATTAGGATTTGGTGAATTAGGGAACTCCTTCATCTACTCTTATTCTTCTTCTTTCGTTTAACAAATATTGGTGTTTTGGCTGAACTCTTTTCCCTTTTTTTTTCTGGTGCCTCAAAAAAAAAAAAAAAAAAAAAAAAGATTTTTTTGAAATCTGGTTTGTGGGGTTGTAAATAGAGCTGGCTTTTGGAATTTCAAATTGTGTCAACTGGGCAGGTATCTGAAGCTTCAGTTAGTAGAAACCGCAGCACCATTATTGTTCCTGCCGGAAGCAACCGGGATGAAGGATGGTCTGCATTTAGAAACATTTTGGCAGAGATCAATGAAGCATCTAGGCTTTTCATACTGCCCAATCAGGTTTCTGCTGCTTATGTCTAGCTTCTCAATCATGCATTTCATGGGCTTACTTCCATCGATTTTCTTTTAACACAAATTACGCCTTTTTCTATTTGCCCAAACCAGGAAAATTCTGAACATTCAGAACGTCTCGCCGGACTTTCGGATGATGTAGGAGCAGGCTTCATATCAGGTCATAGTAGTCAATCTGGTCCAACCTCTGACTTGAATGTAGATAGACAAGTAGACTTGTCAGCTCAAGATGAAATGGGGAATCTGGGTGTTTCAAAAGTTATCCGAGCTGATCAAAAGAGATTCTTTTTCGATCTCGGAAGTAACAACCGGGGTCATTTCTTGAGGATTTCTGAGGTATCGATCAAGAAAACTAATCTTAAGAGCCATGAATTTAATCCCTGTTTAATCTGTTTCTGTCTGTCTTGAACCCCATGGTTCATTTCTTCAGTTTCTTGGTATGAAATTCACTAGAATGATATCGGTTACAGGTTGCAGGGGCAGATCGTTCTTCAATCATTCTCCCATTGTCAGGTCTTAAGCAGTTCTATGAAATAGTAGGACATTTTGTGGAGATCACCAAAGACAGGATTGAAGGAATGACAGGTGTGAATGTCCGAACCGTGGATCCACCTCAGAGATGAATGATATGGGCCCCGGGGGTTTGTGTTTATTCCTTTTAGTGTTTGTGTAGATCTACACGCACGCAGGTGTTTGAGTAGGGTTCAAATTTGTGGTCAGTGTGGTTGTTTGATATTTTCCTGTCTTGATTTTCTAGTTCAAGTCTGAATAATCCATCCTGCTAGAGATTAGCAGAGGGTATGGAAAATGCTTGAGAAGCTTAATTATTCTTCCATGAATAACAAGAGATAAAGAGAGAGGGGAAAGAGAGATATGCTTGTAATTGTCACTTTATAATAATGGTGAGTTCAGGTGTGTTTTGTTCATTTCATTTGCTGTAGTTACCAATGATTTTCACTTCCTTTTCCAGCCTTTCCGTTTTCATCCTGAGATGAAAATTCGAACCTCGTACCTTTCACATGGGTATGTATGTCTCATTTAGTTATATTAGTAAAGTGTTTGTCATTGATTAGATGAGTTGTTGAAATTGTTATGGTATACCTATTTTGGTTGCAAGTTGAAGACTGTTTTCCCAATATATATTTGTTATATATTCATAAA

mRNA sequence

CTTCGAAATATGTTTCTTCTTCTTCATCATCTTTCATTGTCATTTTAATTTCAATTTCCAACTGTTAAAAACCCCATCACAAATCGTTCATGTAATTTCTAACCCTAATTTTCCAGAACCCTTTTGTCAAAATCCTGTTTTTTTTTTTTTTTTTTCTCTTTTTCTCTCATGGGGTTCACTTATTAGATCTCCCATTTCTAGGTTTCTGATTCTTTTCTTGTATTTTGTTTGCATTCAAGGCATTTGGCGGGTTTTTGCATCGATTTCTGTTCGAATCGGGGCTTGTTCTTTTTAATTTATGTGTAATTTTGTCTTTTTTGGTAAACCCAATTGGAGAAAAAATGATGCTGATTTATTGAATTGAGATTCTTGTACGAATTTAGATAATCGCTTAAATCGTGGGGAATTTTAAGTAGAAACCGAGTCAGAGCTGGTAGTGGGAGATGGAGGGGAATTCCGGCGGAGGTGGAGTGGGAATTGGAGGAACGACGACGGGTGGCGTGGCGGCGGGAGGAGGGGCTGGTGGCGGCGGAGGGAATGACGTGGAGTTGATGTGCAAAACACTGCAGGTGGAACACAAGCTGTTTTACTTCGATCTGAAGGAGAATCCCAGAGGTCGATATCTGAAGATTTCAGAGAAAACATCGGCTACAAGGTCAACGATCATTGTTCCTTTCTCTGGGATTCCATGGTTCTTAGATCTCTTCAATTACTATATCAATTCTGATGATCCGGAGGTTTTTAGCAAGGAATTGCAGCTGGACACAAAGGTGTTTTACTTTGACATTGGCGAGAATAGGAGAGGTCGTTTCTTGAAGGTATCTGAAGCTTCAGTTAGTAGAAACCGCAGCACCATTATTGTTCCTGCCGGAAGCAACCGGGATGAAGGATGGTCTGCATTTAGAAACATTTTGGCAGAGATCAATGAAGCATCTAGGCTTTTCATACTGCCCAATCAGGAAAATTCTGAACATTCAGAACGTCTCGCCGGACTTTCGGATGATGTAGGAGCAGGCTTCATATCAGGTCATAGTAGTCAATCTGGTCCAACCTCTGACTTGAATGTAGATAGACAAGTAGACTTGTCAGCTCAAGATGAAATGGGGAATCTGGGTGTTTCAAAAGTTATCCGAGCTGATCAAAAGAGATTCTTTTTCGATCTCGGAAGTAACAACCGGGGTCATTTCTTGAGGATTTCTGAGGTTGCAGGGGCAGATCGTTCTTCAATCATTCTCCCATTGTCAGGTCTTAAGCAGTTCTATGAAATAGTAGGACATTTTGTGGAGATCACCAAAGACAGGATTGAAGGAATGACAGGTGTGAATGTCCGAACCGTGGATCCACCTCAGAGATGAATGATATGGGCCCCGGGGGTTTGTGTTTATTCCTTTTAGTGTTTGTGTAGATCTACACGCACGCAGGTGTTTGAGTAGGGTTCAAATTTGTGGTCAGTGTGGTTGTTTGATATTTTCCTGTCTTGATTTTCTAGTTCAAGTCTGAATAATCCATCCTGCTAGAGATTAGCAGAGGGTATGGAAAATGCTTGAGAAGCTTAATTATTCTTCCATGAATAACAAGAGATAAAGAGAGAGGGGAAAGAGAGATATGCTTGTAATTGTCACTTTATAATAATGGTGAGTTCAGGTGTGTTTTGTTCATTTCATTTGCTGTAGTTACCAATGATTTTCACTTCCTTTTCCAGCCTTTCCGTTTTCATCCTGAGATGAAAATTCGAACCTCGTACCTTTCACATGGGTATGTATGTCTCATTTAGTTATATTAGTAAAGTGTTTGTCATTGATTAGATGAGTTGTTGAAATTGTTATGGTATACCTATTTTGGTTGCAAGTTGAAGACTGTTTTCCCAATATATATTTGTTATATATTCATAAA

Coding sequence (CDS)

ATGGAGGGGAATTCCGGCGGAGGTGGAGTGGGAATTGGAGGAACGACGACGGGTGGCGTGGCGGCGGGAGGAGGGGCTGGTGGCGGCGGAGGGAATGACGTGGAGTTGATGTGCAAAACACTGCAGGTGGAACACAAGCTGTTTTACTTCGATCTGAAGGAGAATCCCAGAGGTCGATATCTGAAGATTTCAGAGAAAACATCGGCTACAAGGTCAACGATCATTGTTCCTTTCTCTGGGATTCCATGGTTCTTAGATCTCTTCAATTACTATATCAATTCTGATGATCCGGAGGTTTTTAGCAAGGAATTGCAGCTGGACACAAAGGTGTTTTACTTTGACATTGGCGAGAATAGGAGAGGTCGTTTCTTGAAGGTATCTGAAGCTTCAGTTAGTAGAAACCGCAGCACCATTATTGTTCCTGCCGGAAGCAACCGGGATGAAGGATGGTCTGCATTTAGAAACATTTTGGCAGAGATCAATGAAGCATCTAGGCTTTTCATACTGCCCAATCAGGAAAATTCTGAACATTCAGAACGTCTCGCCGGACTTTCGGATGATGTAGGAGCAGGCTTCATATCAGGTCATAGTAGTCAATCTGGTCCAACCTCTGACTTGAATGTAGATAGACAAGTAGACTTGTCAGCTCAAGATGAAATGGGGAATCTGGGTGTTTCAAAAGTTATCCGAGCTGATCAAAAGAGATTCTTTTTCGATCTCGGAAGTAACAACCGGGGTCATTTCTTGAGGATTTCTGAGGTTGCAGGGGCAGATCGTTCTTCAATCATTCTCCCATTGTCAGGTCTTAAGCAGTTCTATGAAATAGTAGGACATTTTGTGGAGATCACCAAAGACAGGATTGAAGGAATGACAGGTGTGAATGTCCGAACCGTGGATCCACCTCAGAGATGA

Protein sequence

MEGNSGGGGVGIGGTTTGGVAAGGGAGGGGGNDVELMCKTLQVEHKLFYFDLKENPRGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHSERLAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDPPQR
Homology
BLAST of PI0003412 vs. ExPASy Swiss-Prot
Match: Q9SKZ1 (Transcription factor Pur-alpha 1 OS=Arabidopsis thaliana OX=3702 GN=PURA1 PE=1 SV=2)

HSP 1 Score: 455.3 bits (1170), Expect = 5.4e-127
Identity = 238/304 (78.29%), Postives = 262/304 (86.18%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTTGGVAAGGGAGGGGGNDVELMCKTLQVEHKLFYFDLKENPRGRY 60
           ME NSGG     GG   GG A  GG GGGGG+DVEL+ KTLQVEHKLFYFDLKENPRGRY
Sbjct: 1   MEANSGG-----GGGAEGGRAVTGGGGGGGGSDVELVSKTLQVEHKLFYFDLKENPRGRY 60

Query: 61  LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGENRR 120
           LKISEKTSATRSTIIVP SGI WFLDLFNYY+NS++ E+FSKELQLD+KVFYFDIGENRR
Sbjct: 61  LKISEKTSATRSTIIVPSSGISWFLDLFNYYVNSEEHELFSKELQLDSKVFYFDIGENRR 120

Query: 121 GRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQ-ENSEHSE 180
           GRFLKVSEASVSRNRSTIIVPAGS+ DEGW+AFRNILAEI+EAS LF++PNQ + S+  E
Sbjct: 121 GRFLKVSEASVSRNRSTIIVPAGSSPDEGWAAFRNILAEIHEASGLFVMPNQVKPSDGQE 180

Query: 181 RLAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFD 240
            L    DDVGAGFI GH SQ   +S+ NVDR +D   Q+E G  GVSKVIRADQKRFFFD
Sbjct: 181 HLV---DDVGAGFIPGHGSQQPSSSEHNVDRTIDSPGQEETGMTGVSKVIRADQKRFFFD 240

Query: 241 LGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVD 300
           LG+NNRGHFLRISEVAG+DRSSIILPLSGLKQF+E++GHFVEITKD+IEGMTG NVRTVD
Sbjct: 241 LGNNNRGHFLRISEVAGSDRSSIILPLSGLKQFHEVIGHFVEITKDKIEGMTGANVRTVD 296

Query: 301 PPQR 304
           PPQR
Sbjct: 301 PPQR 296

BLAST of PI0003412 vs. ExPASy Swiss-Prot
Match: Q00577 (Transcriptional activator protein Pur-alpha OS=Homo sapiens OX=9606 GN=PURA PE=1 SV=2)

HSP 1 Score: 87.8 bits (216), Expect = 2.3e-16
Identity = 86/283 (30.39%), Postives = 126/283 (44.52%), Query Frame = 0

Query: 3   GNSGGGGVGIGGTTTGGVAAGGGAGGGGGNDV-ELMCKTLQVEHKLFYFDLKENPRGRYL 62
           G+ GGGG G GG  +GG   GGGA GG  ++  EL  K + +++K FY D+K+N +GR+L
Sbjct: 29  GSGGGGGGGGGGGGSGG--GGGGAPGGLQHETQELASKRVDIQNKRFYLDVKQNAKGRFL 88

Query: 63  KISE-KTSATRSTIIVPFSGIPWFLDLFNYYIN----------------SDDPE--VFSK 122
           KI+E      +S + +  S    F D    +I                  D+P   + S+
Sbjct: 89  KIAEVGAGGNKSRLTLSMSVAVEFRDYLGDFIEHYAQLGPSQPPDLAQAQDEPRRALKSE 148

Query: 123 ELQLDTKVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINE 182
            L  + + +Y D+ EN+RGRFL++        R T+      NR  G  + +        
Sbjct: 149 FLVRENRKYYMDLKENQRGRFLRI--------RQTV------NRGPGLGSTQ-------- 208

Query: 183 ASRLFILPNQENSEHSERLAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGN 242
             +   LP Q   E  + LA L DD G                           ++E   
Sbjct: 209 -GQTIALPAQGLIEFRDALAKLIDDYG--------------------------VEEEPAE 260

Query: 243 LGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILP 266
           L     +  D KRFFFD+GSN  G F+R+SEV    R+SI +P
Sbjct: 269 LPEGTSLTVDNKRFFFDVGSNKYGVFMRVSEVKPTYRNSITVP 260

BLAST of PI0003412 vs. ExPASy Swiss-Prot
Match: P42669 (Transcriptional activator protein Pur-alpha OS=Mus musculus OX=10090 GN=Pura PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 3.0e-16
Identity = 86/283 (30.39%), Postives = 125/283 (44.17%), Query Frame = 0

Query: 3   GNSGGGGVGIGGTTTGGVAAGGGAGGGGGNDV-ELMCKTLQVEHKLFYFDLKENPRGRYL 62
           G+ GGGG G GG   GG   GGGA GG  ++  EL  K + +++K FY D+K+N +GR+L
Sbjct: 29  GSGGGGGGGGGG---GGSGGGGGAPGGLQHETQELASKRVDIQNKRFYLDVKQNAKGRFL 88

Query: 63  KISE-KTSATRSTIIVPFSGIPWFLDLFNYYIN----------------SDDPE--VFSK 122
           KI+E      +S + +  S    F D    +I                  D+P   + S+
Sbjct: 89  KIAEVGAGGNKSRLTLSMSVAVEFRDYLGDFIEHYAQLGPSQPPDLAQAQDEPRRALKSE 148

Query: 123 ELQLDTKVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINE 182
            L  + + +Y D+ EN+RGRFL++        R T+      NR  G  + +        
Sbjct: 149 FLVRENRKYYMDLKENQRGRFLRI--------RQTV------NRGPGLGSTQ-------- 208

Query: 183 ASRLFILPNQENSEHSERLAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGN 242
             +   LP Q   E  + LA L DD G                           ++E   
Sbjct: 209 -GQTIALPAQGLIEFRDALAKLIDDYG--------------------------VEEEPAE 259

Query: 243 LGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILP 266
           L     +  D KRFFFD+GSN  G F+R+SEV    R+SI +P
Sbjct: 269 LPEGTSLTVDNKRFFFDVGSNKYGVFMRVSEVKPTYRNSITVP 259

BLAST of PI0003412 vs. ExPASy Swiss-Prot
Match: Q8AVS4 (Transcriptional activator protein Pur-beta-B OS=Xenopus laevis OX=8355 GN=purb-b PE=2 SV=3)

HSP 1 Score: 87.0 bits (214), Expect = 3.9e-16
Identity = 84/290 (28.97%), Postives = 122/290 (42.07%), Query Frame = 0

Query: 19  GVAAGGGAGGGGG---------NDVELMCKTLQVEHKLFYFDLKENPRGRYLKISE-KTS 78
           G   GG +GG  G            EL  K L +++K FY D+K+N +GR++KI+E    
Sbjct: 7   GSERGGSSGGPSGFSQHMSREQETQELASKRLDIQNKRFYLDVKQNAKGRFIKIAEVGAG 66

Query: 79  ATRSTIIVPFSGIPWFLDLFNYYI------NSDDPE--------------------VFSK 138
            ++S + +  +    F D    +I          PE                    + S+
Sbjct: 67  GSKSRLTLSMAVAAEFRDYLGDFIEHYAQLGPSSPEQIAQASGEDGAGGPGGPRRALKSE 126

Query: 139 ELQLDTKVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINE 198
            L  + + +Y D+ EN+RGRFL++        R TI      NR  G+S        + +
Sbjct: 127 FLVRENRKYYLDLKENQRGRFLRI--------RQTI------NRGPGFSGGTGGGPGL-Q 186

Query: 199 ASRLFILPNQENSEHSERLAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGN 258
           + +   LP Q   E  + LA L DD G     G    SG +                 G 
Sbjct: 187 SGQTIALPAQGLIEFRDALAKLIDDYGGEDDEGMGLGSGASGG-------GAGGGGMYGE 246

Query: 259 LGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQF 273
           L     I  D KRFFFD+GSN  G FLR+SEV  + R+SI +PL    +F
Sbjct: 247 LPEGTSITVDSKRFFFDVGSNKYGVFLRVSEVKPSYRNSITVPLKAWGKF 274

BLAST of PI0003412 vs. ExPASy Swiss-Prot
Match: O35295 (Transcriptional activator protein Pur-beta OS=Mus musculus OX=10090 GN=Purb PE=1 SV=3)

HSP 1 Score: 87.0 bits (214), Expect = 3.9e-16
Identity = 91/300 (30.33%), Postives = 128/300 (42.67%), Query Frame = 0

Query: 3   GNSGGGGVGIGGTTTGGVAAGGGAGGGGGND--VELMCKTLQVEHKLFYFDLKENPRGRY 62
           G  GGGG G GG        GGG GG GG     EL  K L +++K FY D+K+N +GR+
Sbjct: 11  GGGGGGGGGPGGFQPAPRGGGGGGGGPGGEQETQELASKRLDIQNKRFYLDVKQNAKGRF 70

Query: 63  LKISE-KTSATRSTIIVPFSGIPWFLDLFNYYI------NSDDPE--------------- 122
           LKI+E     ++S + +  +    F D    +I          PE               
Sbjct: 71  LKIAEVGAGGSKSRLTLSMAVAAEFRDSLGDFIEHYAQLGPSSPEQLAAGAEEGGGPRRA 130

Query: 123 VFSKELQLDTKVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILA 182
           + S+ L  + + +Y D+ EN+RGRFL++ + +V+R         G     G         
Sbjct: 131 LKSEFLVRENRKYYLDLKENQRGRFLRIRQ-TVNRG------GGGFGGGPGPGGL----- 190

Query: 183 EINEASRLFILPNQENSEHSERLAGLSDDVG------AGFISGHSSQSGPTSDLNVDRQV 242
              ++ +   LP Q   E  + LA L DD G      AG   G     GP   L      
Sbjct: 191 ---QSGQTIALPAQGLIEFRDALAKLIDDYGGDEDELAGGPGG--GAGGPGGGL------ 250

Query: 243 DLSAQDEMGNLGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQF 273
                   G L     I  D KRFFFD+G N  G FLR+SEV  + R++I +P     +F
Sbjct: 251 -------YGELPEGTSITVDSKRFFFDVGCNKYGVFLRVSEVKPSYRNAITVPFKAWGKF 280

BLAST of PI0003412 vs. ExPASy TrEMBL
Match: A0A0A0LZP1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G598870 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 4.0e-165
Identity = 302/303 (99.67%), Postives = 302/303 (99.67%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTTGGVAAGGGAGGGGGNDVELMCKTLQVEHKLFYFDLKENPRGRY 60
           MEGNSGGGGVGIGGTT GGVAAGGGAGGGGGNDVELMCKTLQVEHKLFYFDLKENPRGRY
Sbjct: 1   MEGNSGGGGVGIGGTTAGGVAAGGGAGGGGGNDVELMCKTLQVEHKLFYFDLKENPRGRY 60

Query: 61  LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGENRR 120
           LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGENRR
Sbjct: 61  LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGENRR 120

Query: 121 GRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHSER 180
           GRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHSER
Sbjct: 121 GRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHSER 180

Query: 181 LAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDL 240
           LAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDL
Sbjct: 181 LAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDL 240

Query: 241 GSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDP 300
           GSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDP
Sbjct: 241 GSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDP 300

Query: 301 PQR 304
           PQR
Sbjct: 301 PQR 303

BLAST of PI0003412 vs. ExPASy TrEMBL
Match: A0A1S3BPW8 (transcription factor Pur-alpha 1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492403 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.4e-164
Identity = 300/303 (99.01%), Postives = 301/303 (99.34%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTTGGVAAGGGAGGGGGNDVELMCKTLQVEHKLFYFDLKENPRGRY 60
           MEGNSGGGGVGIGGTT GGVAAGGGAG GGGNDVELMCKTLQVEHKLFYFDLKENPRGRY
Sbjct: 1   MEGNSGGGGVGIGGTTAGGVAAGGGAGSGGGNDVELMCKTLQVEHKLFYFDLKENPRGRY 60

Query: 61  LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGENRR 120
           LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGENRR
Sbjct: 61  LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGENRR 120

Query: 121 GRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHSER 180
           GRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILA+INEASRLFILPNQENSEHSER
Sbjct: 121 GRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILADINEASRLFILPNQENSEHSER 180

Query: 181 LAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDL 240
           LAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDL
Sbjct: 181 LAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDL 240

Query: 241 GSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDP 300
           GSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDP
Sbjct: 241 GSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDP 300

Query: 301 PQR 304
           PQR
Sbjct: 301 PQR 303

BLAST of PI0003412 vs. ExPASy TrEMBL
Match: A0A6J1D0R2 (transcription factor Pur-alpha 1 OS=Momordica charantia OX=3673 GN=LOC111016521 PE=3 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 1.9e-159
Identity = 294/305 (96.39%), Postives = 297/305 (97.38%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTTGGVAAGGG--AGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEGNSGGGGVGIGGTT G VAA GG   GGGGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN 120

Query: 121 RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHS 180
           RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGW+AFRNILAEINEASRLFILPNQENSEHS
Sbjct: 121 RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS 180

Query: 181 ERLAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFF 240
           ER+ GLSDDVGAGFISGHSSQSGP SDLNVDRQVDLSAQD+MGNLGVSKVIRADQKRFFF
Sbjct: 181 ERIVGLSDDVGAGFISGHSSQSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFFF 240

Query: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300
           DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV
Sbjct: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300

Query: 301 DPPQR 304
           DPPQR
Sbjct: 301 DPPQR 305

BLAST of PI0003412 vs. ExPASy TrEMBL
Match: A0A6J1HVP1 (transcription factor Pur-alpha 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467262 PE=3 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 4.3e-159
Identity = 294/307 (95.77%), Postives = 295/307 (96.09%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTTGGVA----AGGGAGGGGGNDVELMCKTLQVEHKLFYFDLKENP 60
           MEGNSGGGGV IGGTT G VA     GGG GGGGGNDVELMCKTLQVEHKLFYFDLKENP
Sbjct: 1   MEGNSGGGGVSIGGTTAGSVAVGGVTGGGGGGGGGNDVELMCKTLQVEHKLFYFDLKENP 60

Query: 61  RGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIG 120
           RGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIG
Sbjct: 61  RGRYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIG 120

Query: 121 ENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSE 180
           ENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGW+AFRNILAEINEASRLFILPNQENSE
Sbjct: 121 ENRRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSE 180

Query: 181 HSERLAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRF 240
           HSERL GLSDDVGAGFISGHSSQ GPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRF
Sbjct: 181 HSERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRF 240

Query: 241 FFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVR 300
           FFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVR
Sbjct: 241 FFDLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVR 300

Query: 301 TVDPPQR 304
           TVDPP R
Sbjct: 301 TVDPPHR 307

BLAST of PI0003412 vs. ExPASy TrEMBL
Match: A0A1S4DZ49 (transcription factor Pur-alpha 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492403 PE=3 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 5.6e-159
Identity = 300/336 (89.29%), Postives = 301/336 (89.58%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTTGGVAAGGGAGGGGGNDVELMCKTLQVEHKLFYFDLKENPRGRY 60
           MEGNSGGGGVGIGGTT GGVAAGGGAG GGGNDVELMCKTLQVEHKLFYFDLKENPRGRY
Sbjct: 1   MEGNSGGGGVGIGGTTAGGVAAGGGAGSGGGNDVELMCKTLQVEHKLFYFDLKENPRGRY 60

Query: 61  LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK----------- 120
           LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTK           
Sbjct: 61  LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKASLFLFFLFCF 120

Query: 121 ----------------------VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRD 180
                                 VFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRD
Sbjct: 121 LVYLFVLVGQLLPFLCTHFAFQVFYFDIGENRRGRFLKVSEASVSRNRSTIIVPAGSNRD 180

Query: 181 EGWSAFRNILAEINEASRLFILPNQENSEHSERLAGLSDDVGAGFISGHSSQSGPTSDLN 240
           EGWSAFRNILA+INEASRLFILPNQENSEHSERLAGLSDDVGAGFISGHSSQSGPTSDLN
Sbjct: 181 EGWSAFRNILADINEASRLFILPNQENSEHSERLAGLSDDVGAGFISGHSSQSGPTSDLN 240

Query: 241 VDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLS 300
           VDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLS
Sbjct: 241 VDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDLGSNNRGHFLRISEVAGADRSSIILPLS 300

Query: 301 GLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDPPQR 304
           GLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDPPQR
Sbjct: 301 GLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDPPQR 336

BLAST of PI0003412 vs. NCBI nr
Match: XP_011660046.1 (transcription factor Pur-alpha 1 [Cucumis sativus] >XP_011660047.1 transcription factor Pur-alpha 1 [Cucumis sativus] >XP_031736036.1 transcription factor Pur-alpha 1 [Cucumis sativus] >KGN66342.1 hypothetical protein Csa_007516 [Cucumis sativus])

HSP 1 Score: 590.5 bits (1521), Expect = 8.2e-165
Identity = 302/303 (99.67%), Postives = 302/303 (99.67%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTTGGVAAGGGAGGGGGNDVELMCKTLQVEHKLFYFDLKENPRGRY 60
           MEGNSGGGGVGIGGTT GGVAAGGGAGGGGGNDVELMCKTLQVEHKLFYFDLKENPRGRY
Sbjct: 1   MEGNSGGGGVGIGGTTAGGVAAGGGAGGGGGNDVELMCKTLQVEHKLFYFDLKENPRGRY 60

Query: 61  LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGENRR 120
           LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGENRR
Sbjct: 61  LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGENRR 120

Query: 121 GRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHSER 180
           GRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHSER
Sbjct: 121 GRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHSER 180

Query: 181 LAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDL 240
           LAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDL
Sbjct: 181 LAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDL 240

Query: 241 GSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDP 300
           GSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDP
Sbjct: 241 GSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDP 300

Query: 301 PQR 304
           PQR
Sbjct: 301 PQR 303

BLAST of PI0003412 vs. NCBI nr
Match: XP_008450979.1 (PREDICTED: transcription factor Pur-alpha 1 isoform X2 [Cucumis melo])

HSP 1 Score: 587.0 bits (1512), Expect = 9.1e-164
Identity = 300/303 (99.01%), Postives = 301/303 (99.34%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTTGGVAAGGGAGGGGGNDVELMCKTLQVEHKLFYFDLKENPRGRY 60
           MEGNSGGGGVGIGGTT GGVAAGGGAG GGGNDVELMCKTLQVEHKLFYFDLKENPRGRY
Sbjct: 1   MEGNSGGGGVGIGGTTAGGVAAGGGAGSGGGNDVELMCKTLQVEHKLFYFDLKENPRGRY 60

Query: 61  LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGENRR 120
           LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGENRR
Sbjct: 61  LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGENRR 120

Query: 121 GRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHSER 180
           GRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILA+INEASRLFILPNQENSEHSER
Sbjct: 121 GRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILADINEASRLFILPNQENSEHSER 180

Query: 181 LAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDL 240
           LAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDL
Sbjct: 181 LAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDL 240

Query: 241 GSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDP 300
           GSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDP
Sbjct: 241 GSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDP 300

Query: 301 PQR 304
           PQR
Sbjct: 301 PQR 303

BLAST of PI0003412 vs. NCBI nr
Match: XP_022147650.1 (transcription factor Pur-alpha 1 [Momordica charantia])

HSP 1 Score: 571.6 bits (1472), Expect = 4.0e-159
Identity = 294/305 (96.39%), Postives = 297/305 (97.38%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTTGGVAAGGG--AGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEGNSGGGGVGIGGTT G VAA GG   GGGGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGNSGGGGVGIGGTTAGAVAAVGGVAGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN 120

Query: 121 RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHS 180
           RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGW+AFRNILAEINEASRLFILPNQENSEHS
Sbjct: 121 RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS 180

Query: 181 ERLAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFF 240
           ER+ GLSDDVGAGFISGHSSQSGP SDLNVDRQVDLSAQD+MGNLGVSKVIRADQKRFFF
Sbjct: 181 ERIVGLSDDVGAGFISGHSSQSGPNSDLNVDRQVDLSAQDDMGNLGVSKVIRADQKRFFF 240

Query: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300
           DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV
Sbjct: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300

Query: 301 DPPQR 304
           DPPQR
Sbjct: 301 DPPQR 305

BLAST of PI0003412 vs. NCBI nr
Match: XP_023531642.1 (transcription factor Pur-alpha 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 571.2 bits (1471), Expect = 5.2e-159
Identity = 294/305 (96.39%), Postives = 295/305 (96.72%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTTGGVAAGG--GAGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEGNSGGGGV IGGTT G VA GG  G GGGGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGNSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN 120

Query: 121 RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHS 180
           RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGW+AFRNILAEINEASRLFILPNQENSEHS
Sbjct: 121 RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWTAFRNILAEINEASRLFILPNQENSEHS 180

Query: 181 ERLAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFF 240
           ERL GLSDDVGAGFISGHSSQ GPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFF
Sbjct: 181 ERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFF 240

Query: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300
           DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV
Sbjct: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300

Query: 301 DPPQR 304
           DPP R
Sbjct: 301 DPPHR 305

BLAST of PI0003412 vs. NCBI nr
Match: KAG6588115.1 (Transcription factor Pur-alpha 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 570.5 bits (1469), Expect = 8.8e-159
Identity = 294/305 (96.39%), Postives = 295/305 (96.72%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTTGGVAAGG--GAGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60
           MEG+SGGGGV IGGTT G VA GG  G GGGGGNDVELMCKTLQVEHKLFYFDLKENPRG
Sbjct: 1   MEGSSGGGGVSIGGTTAGSVAVGGVTGGGGGGGNDVELMCKTLQVEHKLFYFDLKENPRG 60

Query: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN 120
           RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN
Sbjct: 61  RYLKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGEN 120

Query: 121 RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHS 180
           RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHS
Sbjct: 121 RRGRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHS 180

Query: 181 ERLAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFF 240
           ERL GLSDDVGAGFISGHSSQ GPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFF
Sbjct: 181 ERLVGLSDDVGAGFISGHSSQPGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFF 240

Query: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300
           DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV
Sbjct: 241 DLGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTV 300

Query: 301 DPPQR 304
           DPP R
Sbjct: 301 DPPHR 305

BLAST of PI0003412 vs. TAIR 10
Match: AT2G32080.2 (purin-rich alpha 1 )

HSP 1 Score: 459.9 bits (1182), Expect = 1.6e-129
Identity = 238/303 (78.55%), Postives = 262/303 (86.47%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTTGGVAAGGGAGGGGGNDVELMCKTLQVEHKLFYFDLKENPRGRY 60
           ME NSGG     GG   GG A  GG GGGGG+DVEL+ KTLQVEHKLFYFDLKENPRGRY
Sbjct: 1   MEANSGG-----GGGAEGGRAVTGGGGGGGGSDVELVSKTLQVEHKLFYFDLKENPRGRY 60

Query: 61  LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGENRR 120
           LKISEKTSATRSTIIVP SGI WFLDLFNYY+NS++ E+FSKELQLD+KVFYFDIGENRR
Sbjct: 61  LKISEKTSATRSTIIVPSSGISWFLDLFNYYVNSEEHELFSKELQLDSKVFYFDIGENRR 120

Query: 121 GRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQENSEHSER 180
           GRFLKVSEASVSRNRSTIIVPAGS+ DEGW+AFRNILAEI+EAS LF++PNQ+ S+  E 
Sbjct: 121 GRFLKVSEASVSRNRSTIIVPAGSSPDEGWAAFRNILAEIHEASGLFVMPNQKPSDGQEH 180

Query: 181 LAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFDL 240
           L    DDVGAGFI GH SQ   +S+ NVDR +D   Q+E G  GVSKVIRADQKRFFFDL
Sbjct: 181 LV---DDVGAGFIPGHGSQQPSSSEHNVDRTIDSPGQEETGMTGVSKVIRADQKRFFFDL 240

Query: 241 GSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVDP 300
           G+NNRGHFLRISEVAG+DRSSIILPLSGLKQF+E++GHFVEITKD+IEGMTG NVRTVDP
Sbjct: 241 GNNNRGHFLRISEVAGSDRSSIILPLSGLKQFHEVIGHFVEITKDKIEGMTGANVRTVDP 295

Query: 301 PQR 304
           PQR
Sbjct: 301 PQR 295

BLAST of PI0003412 vs. TAIR 10
Match: AT2G32080.1 (purin-rich alpha 1 )

HSP 1 Score: 455.3 bits (1170), Expect = 3.9e-128
Identity = 238/304 (78.29%), Postives = 262/304 (86.18%), Query Frame = 0

Query: 1   MEGNSGGGGVGIGGTTTGGVAAGGGAGGGGGNDVELMCKTLQVEHKLFYFDLKENPRGRY 60
           ME NSGG     GG   GG A  GG GGGGG+DVEL+ KTLQVEHKLFYFDLKENPRGRY
Sbjct: 1   MEANSGG-----GGGAEGGRAVTGGGGGGGGSDVELVSKTLQVEHKLFYFDLKENPRGRY 60

Query: 61  LKISEKTSATRSTIIVPFSGIPWFLDLFNYYINSDDPEVFSKELQLDTKVFYFDIGENRR 120
           LKISEKTSATRSTIIVP SGI WFLDLFNYY+NS++ E+FSKELQLD+KVFYFDIGENRR
Sbjct: 61  LKISEKTSATRSTIIVPSSGISWFLDLFNYYVNSEEHELFSKELQLDSKVFYFDIGENRR 120

Query: 121 GRFLKVSEASVSRNRSTIIVPAGSNRDEGWSAFRNILAEINEASRLFILPNQ-ENSEHSE 180
           GRFLKVSEASVSRNRSTIIVPAGS+ DEGW+AFRNILAEI+EAS LF++PNQ + S+  E
Sbjct: 121 GRFLKVSEASVSRNRSTIIVPAGSSPDEGWAAFRNILAEIHEASGLFVMPNQVKPSDGQE 180

Query: 181 RLAGLSDDVGAGFISGHSSQSGPTSDLNVDRQVDLSAQDEMGNLGVSKVIRADQKRFFFD 240
            L    DDVGAGFI GH SQ   +S+ NVDR +D   Q+E G  GVSKVIRADQKRFFFD
Sbjct: 181 HLV---DDVGAGFIPGHGSQQPSSSEHNVDRTIDSPGQEETGMTGVSKVIRADQKRFFFD 240

Query: 241 LGSNNRGHFLRISEVAGADRSSIILPLSGLKQFYEIVGHFVEITKDRIEGMTGVNVRTVD 300
           LG+NNRGHFLRISEVAG+DRSSIILPLSGLKQF+E++GHFVEITKD+IEGMTG NVRTVD
Sbjct: 241 LGNNNRGHFLRISEVAGSDRSSIILPLSGLKQFHEVIGHFVEITKDKIEGMTGANVRTVD 296

Query: 301 PPQR 304
           PPQR
Sbjct: 301 PPQR 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SKZ15.4e-12778.29Transcription factor Pur-alpha 1 OS=Arabidopsis thaliana OX=3702 GN=PURA1 PE=1 S... [more]
Q005772.3e-1630.39Transcriptional activator protein Pur-alpha OS=Homo sapiens OX=9606 GN=PURA PE=1... [more]
P426693.0e-1630.39Transcriptional activator protein Pur-alpha OS=Mus musculus OX=10090 GN=Pura PE=... [more]
Q8AVS43.9e-1628.97Transcriptional activator protein Pur-beta-B OS=Xenopus laevis OX=8355 GN=purb-b... [more]
O352953.9e-1630.33Transcriptional activator protein Pur-beta OS=Mus musculus OX=10090 GN=Purb PE=1... [more]
Match NameE-valueIdentityDescription
A0A0A0LZP14.0e-16599.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G598870 PE=3 SV=1[more]
A0A1S3BPW84.4e-16499.01transcription factor Pur-alpha 1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492... [more]
A0A6J1D0R21.9e-15996.39transcription factor Pur-alpha 1 OS=Momordica charantia OX=3673 GN=LOC111016521 ... [more]
A0A6J1HVP14.3e-15995.77transcription factor Pur-alpha 1-like OS=Cucurbita maxima OX=3661 GN=LOC11146726... [more]
A0A1S4DZ495.6e-15989.29transcription factor Pur-alpha 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492... [more]
Match NameE-valueIdentityDescription
XP_011660046.18.2e-16599.67transcription factor Pur-alpha 1 [Cucumis sativus] >XP_011660047.1 transcription... [more]
XP_008450979.19.1e-16499.01PREDICTED: transcription factor Pur-alpha 1 isoform X2 [Cucumis melo][more]
XP_022147650.14.0e-15996.39transcription factor Pur-alpha 1 [Momordica charantia][more]
XP_023531642.15.2e-15996.39transcription factor Pur-alpha 1-like [Cucurbita pepo subsp. pepo][more]
KAG6588115.18.8e-15996.39Transcription factor Pur-alpha 1, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
AT2G32080.21.6e-12978.55purin-rich alpha 1 [more]
AT2G32080.13.9e-12878.29purin-rich alpha 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006628Purine-rich element binding protein familySMARTSM00712purcoord: 223..284
e-value: 4.4E-24
score: 96.0
coord: 35..96
e-value: 9.7E-23
score: 91.5
coord: 98..165
e-value: 4.4E-25
score: 99.3
IPR006628Purine-rich element binding protein familyPFAMPF04845PurAcoord: 36..162
e-value: 1.0E-19
score: 70.7
coord: 217..279
e-value: 6.9E-13
score: 48.4
IPR006628Purine-rich element binding protein familyPANTHERPTHR12611PUR-TRANSCRIPTIONAL ACTIVATORcoord: 26..290
NoneNo IPR availableGENE3D3.10.450.700coord: 25..95
e-value: 1.7E-18
score: 67.9
coord: 213..287
e-value: 2.3E-19
score: 70.8
coord: 96..165
e-value: 9.5E-22
score: 78.4
NoneNo IPR availablePANTHERPTHR12611:SF0PURINE-RICH BINDING PROTEIN-ALPHA, ISOFORM Bcoord: 26..290

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0003412.1PI0003412.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function GO:0003729 mRNA binding
molecular_function GO:0032422 purine-rich negative regulatory element binding
molecular_function GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding