PI0003250 (gene) Melon (PI 482460) v1

BLAST of PI0003250 vs. ExPASy Swiss-Prot
Match: Q7YR40 (Mediator of DNA damage checkpoint protein 1 OS=Pan troglodytes OX=9598 GN=MDC1 PE=3 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 7.9e-19
Identity = 48/175 (27.43%), Postives = 93/175 (53.14%), Query Frame = 0

Query: 458  KVIEELGGSLTADGCTSTHVITGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDEL 517
            + +  LGGSL      ++H++T ++R+T+ F  AL  G  I+S  WL +S++ G F+   
Sbjct: 1989 RAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSHKAGFFLPPD 2048

Query: 518  PYILNDDDYTSKYRASLKTAVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGN 577
             Y++ D +    +  SL+ A+ RA+ R   L EGY++ ++   QPPP  +  I+   GG 
Sbjct: 2049 EYVVTDPEQEKNFGFSLQDALSRARER--RLLEGYEIYVTPGVQPPPPQMGEIISCCGGT 2108

Query: 578  VIHELDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWVMTCIMRQEVDLEA 633
             +  + +     + + + C +D     + +  G+   + E+++T +++QE   EA
Sbjct: 2109 YLPSMPRSYKPQRVV-ITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEA 2160

BLAST of PI0003250 vs. ExPASy Swiss-Prot
Match: Q767L8 (Mediator of DNA damage checkpoint protein 1 OS=Sus scrofa OX=9823 GN=MDC1 PE=3 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 7.9e-19
Identity = 50/175 (28.57%), Postives = 93/175 (53.14%), Query Frame = 0

Query: 458  KVIEELGGSLTADGCTSTHVITGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDEL 517
            + +  LGGSL +    ++H++T ++R+T+ F  AL  G  I+S  WL +S + G F+   
Sbjct: 1860 RAVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGCFLPPD 1919

Query: 518  PYILNDDDYTSKYRASLKTAVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGN 577
             Y++ D +    +  SL+ A+ RA+ R   L EGY++ ++   QPPP  +  I+   GG 
Sbjct: 1920 EYVVTDPEQEKNFGFSLREALSRARER--KLLEGYEIHVTPGVQPPPPQMGEIISCCGGT 1979

Query: 578  VIHELDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWVMTCIMRQEVDLEA 633
            V+  + +     + + + C +D     V    G+   + E+++T +++QE   EA
Sbjct: 1980 VLPSMPRSYKPQRVV-ITCSQDFPRCSVPFRLGLPVLSPEFLLTGVLKQEAKPEA 2031

BLAST of PI0003250 vs. ExPASy Swiss-Prot
Match: Q5TM68 (Mediator of DNA damage checkpoint protein 1 OS=Macaca mulatta OX=9544 GN=MDC1 PE=3 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 1.3e-18
Identity = 52/192 (27.08%), Postives = 98/192 (51.04%), Query Frame = 0

Query: 441  KIMLMNIADEAKKTQLMKVIEELGGSLTADGCTSTHVITGKVRKTLNFCTALCSGAWIVS 500
            K++   + D     Q  + +  LGGSL      ++H++T ++R+T+ F  AL  G  I+S
Sbjct: 1978 KVLFTGVVD----AQGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILS 2037

Query: 501  PSWLKESYREGRFVDELPYILNDDDYTSKYRASLKTAVLRAKARPGALFEGYDVCISAHA 560
              WL +S + G F+    Y++ D +    +  SL+ A+ RA+ R   L EGY++ ++   
Sbjct: 2038 LDWLHQSRKAGCFLPPDEYVVTDPEQEKNFGFSLQDALSRARER--RLLEGYEIYVTPGV 2097

Query: 561  QPPPKTLSLIVKSAGGNVIHELDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWVM 620
            QPPP  +  I+   GG  +  + +     + + + C +D     V +  G+   + E+++
Sbjct: 2098 QPPPPQMGEIISCCGGTYLPSMPRSYKPQRVV-ITCPQDFPRCSVPLRVGLPLLSPEFLL 2157

Query: 621  TCIMRQEVDLEA 633
            T +++QE   EA
Sbjct: 2158 TGVLKQEAKPEA 2162

BLAST of PI0003250 vs. ExPASy Swiss-Prot
Match: Q6ZW49 (PAX-interacting protein 1 OS=Homo sapiens OX=9606 GN=PAXIP1 PE=1 SV=2)

HSP 1 Score: 95.9 bits (237), Expect = 1.8e-18
Identity = 60/190 (31.58%), Postives = 98/190 (51.58%), Query Frame = 0

Query: 455  QLMKVIEELGGSLTADGCTSTHVITGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFV 514
            Q +K +  LGG +       TH+I  KV +T+ F TA+     IV+P WL+E +R  +F+
Sbjct: 881  QYIKKLYILGGEVAESAQKCTHLIASKVTRTVKFLTAISVVKHIVTPEWLEECFRCQKFI 940

Query: 515  DELPYILNDDDYTSKYRASLKTAVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSA 574
            DE  YIL D +    +  SL+ ++ RA   P  LF+     I+    P   T+  IV+ A
Sbjct: 941  DEQNYILRDAEAEVLFSFSLEESLKRAHVSP--LFKAKYFYITPGICPSLSTMKAIVECA 1000

Query: 575  GGNVIH---------ELDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWVMTCIMR 634
            GG V+          E  + +++S+ I ++CE D+        +GI   N E+V+T ++ 
Sbjct: 1001 GGKVLSKQPSFRKLMEHKQNSSLSEIILISCENDLHLCREYFARGIDVHNAEFVLTGVLT 1060

Query: 635  QEVDLEAPQF 636
            Q +D E+ +F
Sbjct: 1061 QTLDYESYKF 1068

BLAST of PI0003250 vs. ExPASy Swiss-Prot
Match: Q14676 (Mediator of DNA damage checkpoint protein 1 OS=Homo sapiens OX=9606 GN=MDC1 PE=1 SV=3)

HSP 1 Score: 95.5 bits (236), Expect = 2.3e-18
Identity = 48/175 (27.43%), Postives = 92/175 (52.57%), Query Frame = 0

Query: 458  KVIEELGGSLTADGCTSTHVITGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDEL 517
            + +  LGGSL      ++H++T ++R+T+ F  AL  G  I+S  WL +S + G F+   
Sbjct: 1907 RAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPD 1966

Query: 518  PYILNDDDYTSKYRASLKTAVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGN 577
             Y++ D +    +  SL+ A+ RA+ R   L EGY++ ++   QPPP  +  I+   GG 
Sbjct: 1967 EYVVTDPEQEKNFGFSLQDALSRARER--RLLEGYEIYVTPGVQPPPPQMGEIISCCGGT 2026

Query: 578  VIHELDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWVMTCIMRQEVDLEA 633
             +  + +     + + + C +D     + +  G+   + E+++T +++QE   EA
Sbjct: 2027 YLPSMPRSYKPQRVV-ITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEA 2078

BLAST of PI0003250 vs. ExPASy TrEMBL
Match: A0A1S3BKX0 (uncharacterized protein LOC103491160 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103491160 PE=4 SV=1)

HSP 1 Score: 1187.9 bits (3072), Expect = 0.0e+00
Identity = 598/640 (93.44%), Postives = 612/640 (95.62%), Query Frame = 0

Query: 1   MAPRKKPTRRSSI-PIGKEGHAVDVNHPECVGKGVGEKCHSQGEYSFVLVNPNDFDGHSK 60
           MAPRKKPTRRSSI  +GKEGHAVDVNH ECVGKGVGEKC SQGEYSFVLVNPNDFD +SK
Sbjct: 1   MAPRKKPTRRSSIFIVGKEGHAVDVNHHECVGKGVGEKCRSQGEYSFVLVNPNDFDSYSK 60

Query: 61  SYLQEVLQLFKRELPTMAYAADTGKQSTFMEKCVSNGKYCTLLLESKSEVDPGLVIAAIT 120
           SYLQ+VLQL+KRELPTMAYAA+TGKQSTFMEKC+SNGKYCTLLLESKSEVD GLVIAAIT
Sbjct: 61  SYLQDVLQLYKRELPTMAYAANTGKQSTFMEKCLSNGKYCTLLLESKSEVDSGLVIAAIT 120

Query: 121 YQIVPADTQYAEIPLAAVCSAYQHKGFGHILYMELRKRLQSVGIRTILCWGDKESEGFWS 180
           YQIVPADTQYAEIPLAAV  AYQHKGFGHILYMELRKRLQSVGIRTI CWGDKESEGFWS
Sbjct: 121 YQIVPADTQYAEIPLAAVSLAYQHKGFGHILYMELRKRLQSVGIRTIFCWGDKESEGFWS 180

Query: 181 KQGFSSIAEVDTKGKARRIPVRADIRRALCFPGGSTLMISHIQGISMCSADLPKLLSLLK 240
           KQGF SIAEVDTKGK RRIPVRADIRRALCFPGGSTLMISHIQGISMCSADLPKL SLLK
Sbjct: 181 KQGFLSIAEVDTKGKVRRIPVRADIRRALCFPGGSTLMISHIQGISMCSADLPKLRSLLK 240

Query: 241 PEAPSSYACAARISVSNRGCNVSNETDQHTIQILNFQPDEFVTLVPLGEENEIQDPQNQD 300
           PEAP     AARISV+++GCNVSN TDQHTIQILNFQPDEFVTLVPLGEENEIQDPQNQD
Sbjct: 241 PEAP----YAARISVASQGCNVSNATDQHTIQILNFQPDEFVTLVPLGEENEIQDPQNQD 300

Query: 301 AVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCSCSTQSAKRVWEASLSSLKSKKV 360
           AVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCSCS QSAKRVWEASLSSLKSKKV
Sbjct: 301 AVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCSCSKQSAKRVWEASLSSLKSKKV 360

Query: 361 KGVSLDHFHSDSNKKLVPKSNGYDTCIQACSLANSKHEILSSIYPKNPTNKYTQNFCQEF 420
           KGV+LDHFHSDSNK LVPKSNGY+ C QACSLANSKHEILSSIYPK PTNKYTQNFCQEF
Sbjct: 361 KGVNLDHFHSDSNKNLVPKSNGYNACSQACSLANSKHEILSSIYPKKPTNKYTQNFCQEF 420

Query: 421 RSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLMKVIEELGGSLTADGCTSTHVIT 480
            SVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLMKVIEELGGSLTADG TSTHVIT
Sbjct: 421 GSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLMKVIEELGGSLTADGSTSTHVIT 480

Query: 481 GKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDELPYILNDDDYTSKYRASLKTAVL 540
           GKVRKTLNFCTALCSGAWIVS SWLKESYREGRFVDELP ILNDDDYTSKYRASLKT VL
Sbjct: 481 GKVRKTLNFCTALCSGAWIVSSSWLKESYREGRFVDELPCILNDDDYTSKYRASLKTTVL 540

Query: 541 RAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGNVIHELDKVNNVSKTIFVACEED 600
           RAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGG+VIH+LDKVNNVSKTIFVACEED
Sbjct: 541 RAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGHVIHKLDKVNNVSKTIFVACEED 600

Query: 601 VEEALVAVEKGIWTFNIEWVMTCIMRQEVDLEAPQFAESL 640
           VEEALVAVEKGIWTFNIEW+MTCIMRQEVDLEAPQFAESL
Sbjct: 601 VEEALVAVEKGIWTFNIEWLMTCIMRQEVDLEAPQFAESL 636

BLAST of PI0003250 vs. ExPASy TrEMBL
Match: A0A1S3BMF3 (uncharacterized protein LOC103491160 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491160 PE=4 SV=1)

HSP 1 Score: 1179.5 bits (3050), Expect = 0.0e+00
Identity = 599/662 (90.48%), Postives = 612/662 (92.45%), Query Frame = 0

Query: 1   MAPRKKPTRRSSIPIGKEGHAVDVNHPECVGKGVGEKCHSQGEYSFVLVNPNDFDGHSKS 60
           MAPRKKPTRRSSI IGKEGHAVDVNH ECVGKGVGEKC SQGEYSFVLVNPNDFD +SKS
Sbjct: 1   MAPRKKPTRRSSIFIGKEGHAVDVNHHECVGKGVGEKCRSQGEYSFVLVNPNDFDSYSKS 60

Query: 61  YLQEVLQLFKRELPTMAYAADTGKQSTFMEKCVSNGKYCTLLLESKSEVDPGLVIAAITY 120
           YLQ+VLQL+KRELPTMAYAA+TGKQSTFMEKC+SNGKYCTLLLESKSEVD GLVIAAITY
Sbjct: 61  YLQDVLQLYKRELPTMAYAANTGKQSTFMEKCLSNGKYCTLLLESKSEVDSGLVIAAITY 120

Query: 121 QIVPADTQYAEIPLAAVCSAYQHK-----------------------GFGHILYMELRKR 180
           QIVPADTQYAEIPLAAV  AYQHK                       GFGHILYMELRKR
Sbjct: 121 QIVPADTQYAEIPLAAVSLAYQHKVSLIASTPSPTGGRVGCSVCLFQGFGHILYMELRKR 180

Query: 181 LQSVGIRTILCWGDKESEGFWSKQGFSSIAEVDTKGKARRIPVRADIRRALCFPGGSTLM 240
           LQSVGIRTI CWGDKESEGFWSKQGF SIAEVDTKGK RRIPVRADIRRALCFPGGSTLM
Sbjct: 181 LQSVGIRTIFCWGDKESEGFWSKQGFLSIAEVDTKGKVRRIPVRADIRRALCFPGGSTLM 240

Query: 241 ISHIQGISMCSADLPKLLSLLKPEAPSSYACAARISVSNRGCNVSNETDQHTIQILNFQP 300
           ISHIQGISMCSADLPKL SLLKPEAP     AARISV+++GCNVSN TDQHTIQILNFQP
Sbjct: 241 ISHIQGISMCSADLPKLRSLLKPEAP----YAARISVASQGCNVSNATDQHTIQILNFQP 300

Query: 301 DEFVTLVPLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCS 360
           DEFVTLVPLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCS
Sbjct: 301 DEFVTLVPLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCS 360

Query: 361 CSTQSAKRVWEASLSSLKSKKVKGVSLDHFHSDSNKKLVPKSNGYDTCIQACSLANSKHE 420
           CS QSAKRVWEASLSSLKSKKVKGV+LDHFHSDSNK LVPKSNGY+ C QACSLANSKHE
Sbjct: 361 CSKQSAKRVWEASLSSLKSKKVKGVNLDHFHSDSNKNLVPKSNGYNACSQACSLANSKHE 420

Query: 421 ILSSIYPKNPTNKYTQNFCQEFRSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLM 480
           ILSSIYPK PTNKYTQNFCQEF SVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLM
Sbjct: 421 ILSSIYPKKPTNKYTQNFCQEFGSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLM 480

Query: 481 KVIEELGGSLTADGCTSTHVITGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDEL 540
           KVIEELGGSLTADG TSTHVITGKVRKTLNFCTALCSGAWIVS SWLKESYREGRFVDEL
Sbjct: 481 KVIEELGGSLTADGSTSTHVITGKVRKTLNFCTALCSGAWIVSSSWLKESYREGRFVDEL 540

Query: 541 PYILNDDDYTSKYRASLKTAVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGN 600
           P ILNDDDYTSKYRASLKT VLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGG+
Sbjct: 541 PCILNDDDYTSKYRASLKTTVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGH 600

Query: 601 VIHELDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWVMTCIMRQEVDLEAPQFAE 640
           VIH+LDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEW+MTCIMRQEVDLEAPQFAE
Sbjct: 601 VIHKLDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWLMTCIMRQEVDLEAPQFAE 658

BLAST of PI0003250 vs. ExPASy TrEMBL
Match: A0A1S3BM51 (uncharacterized protein LOC103491160 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491160 PE=4 SV=1)

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 598/663 (90.20%), Postives = 612/663 (92.31%), Query Frame = 0

Query: 1   MAPRKKPTRRSSI-PIGKEGHAVDVNHPECVGKGVGEKCHSQGEYSFVLVNPNDFDGHSK 60
           MAPRKKPTRRSSI  +GKEGHAVDVNH ECVGKGVGEKC SQGEYSFVLVNPNDFD +SK
Sbjct: 1   MAPRKKPTRRSSIFIVGKEGHAVDVNHHECVGKGVGEKCRSQGEYSFVLVNPNDFDSYSK 60

Query: 61  SYLQEVLQLFKRELPTMAYAADTGKQSTFMEKCVSNGKYCTLLLESKSEVDPGLVIAAIT 120
           SYLQ+VLQL+KRELPTMAYAA+TGKQSTFMEKC+SNGKYCTLLLESKSEVD GLVIAAIT
Sbjct: 61  SYLQDVLQLYKRELPTMAYAANTGKQSTFMEKCLSNGKYCTLLLESKSEVDSGLVIAAIT 120

Query: 121 YQIVPADTQYAEIPLAAVCSAYQHK-----------------------GFGHILYMELRK 180
           YQIVPADTQYAEIPLAAV  AYQHK                       GFGHILYMELRK
Sbjct: 121 YQIVPADTQYAEIPLAAVSLAYQHKVSLIASTPSPTGGRVGCSVCLFQGFGHILYMELRK 180

Query: 181 RLQSVGIRTILCWGDKESEGFWSKQGFSSIAEVDTKGKARRIPVRADIRRALCFPGGSTL 240
           RLQSVGIRTI CWGDKESEGFWSKQGF SIAEVDTKGK RRIPVRADIRRALCFPGGSTL
Sbjct: 181 RLQSVGIRTIFCWGDKESEGFWSKQGFLSIAEVDTKGKVRRIPVRADIRRALCFPGGSTL 240

Query: 241 MISHIQGISMCSADLPKLLSLLKPEAPSSYACAARISVSNRGCNVSNETDQHTIQILNFQ 300
           MISHIQGISMCSADLPKL SLLKPEAP     AARISV+++GCNVSN TDQHTIQILNFQ
Sbjct: 241 MISHIQGISMCSADLPKLRSLLKPEAP----YAARISVASQGCNVSNATDQHTIQILNFQ 300

Query: 301 PDEFVTLVPLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHC 360
           PDEFVTLVPLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHC
Sbjct: 301 PDEFVTLVPLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHC 360

Query: 361 SCSTQSAKRVWEASLSSLKSKKVKGVSLDHFHSDSNKKLVPKSNGYDTCIQACSLANSKH 420
           SCS QSAKRVWEASLSSLKSKKVKGV+LDHFHSDSNK LVPKSNGY+ C QACSLANSKH
Sbjct: 361 SCSKQSAKRVWEASLSSLKSKKVKGVNLDHFHSDSNKNLVPKSNGYNACSQACSLANSKH 420

Query: 421 EILSSIYPKNPTNKYTQNFCQEFRSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQL 480
           EILSSIYPK PTNKYTQNFCQEF SVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQL
Sbjct: 421 EILSSIYPKKPTNKYTQNFCQEFGSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQL 480

Query: 481 MKVIEELGGSLTADGCTSTHVITGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDE 540
           MKVIEELGGSLTADG TSTHVITGKVRKTLNFCTALCSGAWIVS SWLKESYREGRFVDE
Sbjct: 481 MKVIEELGGSLTADGSTSTHVITGKVRKTLNFCTALCSGAWIVSSSWLKESYREGRFVDE 540

Query: 541 LPYILNDDDYTSKYRASLKTAVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGG 600
           LP ILNDDDYTSKYRASLKT VLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGG
Sbjct: 541 LPCILNDDDYTSKYRASLKTTVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGG 600

Query: 601 NVIHELDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWVMTCIMRQEVDLEAPQFA 640
           +VIH+LDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEW+MTCIMRQEVDLEAPQFA
Sbjct: 601 HVIHKLDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWLMTCIMRQEVDLEAPQFA 659

BLAST of PI0003250 vs. ExPASy TrEMBL
Match: A0A1S4DXT4 (uncharacterized protein LOC103491160 isoform X4 OS=Cucumis melo OX=3656 GN=LOC103491160 PE=4 SV=1)

HSP 1 Score: 1062.0 bits (2745), Expect = 9.9e-307
Identity = 541/600 (90.17%), Postives = 554/600 (92.33%), Query Frame = 0

Query: 63  QEVLQLFKRELPTMAYAADTGKQSTFMEKCVSNGKYCTLLLESKSEVDPGLVIAAITYQI 122
           ++VLQL+KRELPTMAYAA+TGKQSTFMEKC+SNGKYCTLLLESKSEVD GLVIAAITYQI
Sbjct: 13  KDVLQLYKRELPTMAYAANTGKQSTFMEKCLSNGKYCTLLLESKSEVDSGLVIAAITYQI 72

Query: 123 VPADTQYAEIPLAAVCSAYQHK-----------------------GFGHILYMELRKRLQ 182
           VPADTQYAEIPLAAV  AYQHK                       GFGHILYMELRKRLQ
Sbjct: 73  VPADTQYAEIPLAAVSLAYQHKVSLIASTPSPTGGRVGCSVCLFQGFGHILYMELRKRLQ 132

Query: 183 SVGIRTILCWGDKESEGFWSKQGFSSIAEVDTKGKARRIPVRADIRRALCFPGGSTLMIS 242
           SVGIRTI CWGDKESEGFWSKQGF SIAEVDTKGK RRIPVRADIRRALCFPGGSTLMIS
Sbjct: 133 SVGIRTIFCWGDKESEGFWSKQGFLSIAEVDTKGKVRRIPVRADIRRALCFPGGSTLMIS 192

Query: 243 HIQGISMCSADLPKLLSLLKPEAPSSYACAARISVSNRGCNVSNETDQHTIQILNFQPDE 302
           HIQGISMCSADLPKL SLLKPEAP     AARISV+++GCNVSN TDQHTIQILNFQPDE
Sbjct: 193 HIQGISMCSADLPKLRSLLKPEAP----YAARISVASQGCNVSNATDQHTIQILNFQPDE 252

Query: 303 FVTLVPLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCSCS 362
           FVTLVPLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCSCS
Sbjct: 253 FVTLVPLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCSCS 312

Query: 363 TQSAKRVWEASLSSLKSKKVKGVSLDHFHSDSNKKLVPKSNGYDTCIQACSLANSKHEIL 422
            QSAKRVWEASLSSLKSKKVKGV+LDHFHSDSNK LVPKSNGY+ C QACSLANSKHEIL
Sbjct: 313 KQSAKRVWEASLSSLKSKKVKGVNLDHFHSDSNKNLVPKSNGYNACSQACSLANSKHEIL 372

Query: 423 SSIYPKNPTNKYTQNFCQEFRSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLMKV 482
           SSIYPK PTNKYTQNFCQEF SVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLMKV
Sbjct: 373 SSIYPKKPTNKYTQNFCQEFGSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLMKV 432

Query: 483 IEELGGSLTADGCTSTHVITGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDELPY 542
           IEELGGSLTADG TSTHVITGKVRKTLNFCTALCSGAWIVS SWLKESYREGRFVDELP 
Sbjct: 433 IEELGGSLTADGSTSTHVITGKVRKTLNFCTALCSGAWIVSSSWLKESYREGRFVDELPC 492

Query: 543 ILNDDDYTSKYRASLKTAVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGNVI 602
           ILNDDDYTSKYRASLKT VLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGG+VI
Sbjct: 493 ILNDDDYTSKYRASLKTTVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGHVI 552

Query: 603 HELDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWVMTCIMRQEVDLEAPQFAESL 640
           H+LDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEW+MTCIMRQEVDLEAPQFAESL
Sbjct: 553 HKLDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWLMTCIMRQEVDLEAPQFAESL 608

BLAST of PI0003250 vs. ExPASy TrEMBL
Match: A0A6J1HT38 (uncharacterized protein LOC111465956 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465956 PE=4 SV=1)

HSP 1 Score: 1014.6 bits (2622), Expect = 1.8e-292
Identity = 516/643 (80.25%), Postives = 560/643 (87.09%), Query Frame = 0

Query: 1   MAPRKKPTRRSSIPI-GKEGHAVDVNHPECVGKGVGEKCHSQGEYSFVLVNPNDFDGHSK 60
           MA  ++P RRS  PI GKEGH VD N PEC   G+GEKCHSQGEYSFVLVNPN+FD  SK
Sbjct: 1   MARTQRPPRRSLTPIVGKEGHVVDANPPECDETGMGEKCHSQGEYSFVLVNPNEFDSVSK 60

Query: 61  SYLQEVLQLFKRELPTMAYAADTGKQSTFMEKCVSNGKYCTLLLESKSEVDPGLVIAAIT 120
           SYLQ VL L+KRELP M YAA+TGKQSTFMEKCVSNGKYCTLLL+S SE DPG +IAAIT
Sbjct: 61  SYLQAVLLLYKRELPAMVYAANTGKQSTFMEKCVSNGKYCTLLLKSTSEADPGTIIAAIT 120

Query: 121 YQIVPADTQYAEIPLAAVCSAYQHKGFGHILYMELRKRLQSVGIRTILCWGDKESEGFWS 180
           YQIVPADTQYAEIPLAAV SAYQHKGF  ILYMELRKRLQSVGIRTI CWGDKESEGFWS
Sbjct: 121 YQIVPADTQYAEIPLAAVSSAYQHKGFCRILYMELRKRLQSVGIRTIFCWGDKESEGFWS 180

Query: 181 KQGFSSIAEVDTKGKARRIPVRADIRRALCFPGGSTLMISHI-QGISMCSADLPKLLSLL 240
           KQGF SIAEVDTKGKARRIPVRADIRRALCFPGGSTLM+SHI QG S CSAD PK  +LL
Sbjct: 181 KQGFLSIAEVDTKGKARRIPVRADIRRALCFPGGSTLMVSHIYQGSSTCSADRPKSRTLL 240

Query: 241 KPEAPSSYACAARISVSNRGCNVSNETDQHTIQILNFQPDEFVTLVPLGEENEIQDPQNQ 300
           KP+ P SY  A R SV+++GC VSN  DQ T + LNFQP+EF +LVPLG EN+I D QNQ
Sbjct: 241 KPDVPISYELAKRTSVASQGCKVSNAIDQQTTENLNFQPEEFASLVPLGGENKIHDSQNQ 300

Query: 301 DAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCSCSTQSAKRVWEASLSSLKSKK 360
           DAV D NGPV FAE+EN  TAS AEL+  IGNLDE  CSCSTQ AKRVWEASLSSLKSKK
Sbjct: 301 DAVPDCNGPVFFAEVENRKTASNAELTKNIGNLDEIQCSCSTQRAKRVWEASLSSLKSKK 360

Query: 361 VKGVSLDHFHSDSNKKLVPKSNGYDTCIQACSLANSKHEILSSIYPKNP-TNKYTQNFCQ 420
           VKGV L H   DS +  VP+SNG DTC QACSLANSKHEIL+S+ PKNP T+KYTQNFC+
Sbjct: 361 VKGVHLQHSRPDSIRSFVPESNGCDTCSQACSLANSKHEILASVPPKNPSTSKYTQNFCE 420

Query: 421 EFRSVNVASEDLNCEE-NTLGKSFKIMLMNIADEAKKTQLMKVIEELGGSLTADGCTSTH 480
           E  SVNVASEDL  +  +TLGKSF+IMLMNIADEAKKTQL+KVIEELGGSLT+DG TSTH
Sbjct: 421 EIGSVNVASEDLVSKGLHTLGKSFRIMLMNIADEAKKTQLVKVIEELGGSLTSDGSTSTH 480

Query: 481 VITGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDELPYILNDDDYTSKYRASLKT 540
           VITGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDE PYILNDDDYTSKYRASLKT
Sbjct: 481 VITGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDEFPYILNDDDYTSKYRASLKT 540

Query: 541 AVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGNVIHELDKVNNVSKTIFVAC 600
           AVLRAKARPGALFEGYDVCISAHAQPPPKTLS+IVKSAGGNVI+ L KV+ VS+TIFVAC
Sbjct: 541 AVLRAKARPGALFEGYDVCISAHAQPPPKTLSVIVKSAGGNVINGLGKVSRVSETIFVAC 600

Query: 601 EEDVEEALVAVEKGIWTFNIEWVMTCIMRQEVDLEAPQFAESL 640
           EEDVEEAL+A+++GIWTFN EW+M+C+MRQE+D+EAPQFAESL
Sbjct: 601 EEDVEEALMAIKEGIWTFNSEWLMSCVMRQELDMEAPQFAESL 643

BLAST of PI0003250 vs. NCBI nr
Match: XP_008449211.1 (PREDICTED: uncharacterized protein LOC103491160 isoform X3 [Cucumis melo])

HSP 1 Score: 1187.9 bits (3072), Expect = 0.0e+00
Identity = 598/640 (93.44%), Postives = 612/640 (95.62%), Query Frame = 0

Query: 1   MAPRKKPTRRSSI-PIGKEGHAVDVNHPECVGKGVGEKCHSQGEYSFVLVNPNDFDGHSK 60
           MAPRKKPTRRSSI  +GKEGHAVDVNH ECVGKGVGEKC SQGEYSFVLVNPNDFD +SK
Sbjct: 1   MAPRKKPTRRSSIFIVGKEGHAVDVNHHECVGKGVGEKCRSQGEYSFVLVNPNDFDSYSK 60

Query: 61  SYLQEVLQLFKRELPTMAYAADTGKQSTFMEKCVSNGKYCTLLLESKSEVDPGLVIAAIT 120
           SYLQ+VLQL+KRELPTMAYAA+TGKQSTFMEKC+SNGKYCTLLLESKSEVD GLVIAAIT
Sbjct: 61  SYLQDVLQLYKRELPTMAYAANTGKQSTFMEKCLSNGKYCTLLLESKSEVDSGLVIAAIT 120

Query: 121 YQIVPADTQYAEIPLAAVCSAYQHKGFGHILYMELRKRLQSVGIRTILCWGDKESEGFWS 180
           YQIVPADTQYAEIPLAAV  AYQHKGFGHILYMELRKRLQSVGIRTI CWGDKESEGFWS
Sbjct: 121 YQIVPADTQYAEIPLAAVSLAYQHKGFGHILYMELRKRLQSVGIRTIFCWGDKESEGFWS 180

Query: 181 KQGFSSIAEVDTKGKARRIPVRADIRRALCFPGGSTLMISHIQGISMCSADLPKLLSLLK 240
           KQGF SIAEVDTKGK RRIPVRADIRRALCFPGGSTLMISHIQGISMCSADLPKL SLLK
Sbjct: 181 KQGFLSIAEVDTKGKVRRIPVRADIRRALCFPGGSTLMISHIQGISMCSADLPKLRSLLK 240

Query: 241 PEAPSSYACAARISVSNRGCNVSNETDQHTIQILNFQPDEFVTLVPLGEENEIQDPQNQD 300
           PEAP     AARISV+++GCNVSN TDQHTIQILNFQPDEFVTLVPLGEENEIQDPQNQD
Sbjct: 241 PEAP----YAARISVASQGCNVSNATDQHTIQILNFQPDEFVTLVPLGEENEIQDPQNQD 300

Query: 301 AVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCSCSTQSAKRVWEASLSSLKSKKV 360
           AVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCSCS QSAKRVWEASLSSLKSKKV
Sbjct: 301 AVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCSCSKQSAKRVWEASLSSLKSKKV 360

Query: 361 KGVSLDHFHSDSNKKLVPKSNGYDTCIQACSLANSKHEILSSIYPKNPTNKYTQNFCQEF 420
           KGV+LDHFHSDSNK LVPKSNGY+ C QACSLANSKHEILSSIYPK PTNKYTQNFCQEF
Sbjct: 361 KGVNLDHFHSDSNKNLVPKSNGYNACSQACSLANSKHEILSSIYPKKPTNKYTQNFCQEF 420

Query: 421 RSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLMKVIEELGGSLTADGCTSTHVIT 480
            SVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLMKVIEELGGSLTADG TSTHVIT
Sbjct: 421 GSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLMKVIEELGGSLTADGSTSTHVIT 480

Query: 481 GKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDELPYILNDDDYTSKYRASLKTAVL 540
           GKVRKTLNFCTALCSGAWIVS SWLKESYREGRFVDELP ILNDDDYTSKYRASLKT VL
Sbjct: 481 GKVRKTLNFCTALCSGAWIVSSSWLKESYREGRFVDELPCILNDDDYTSKYRASLKTTVL 540

Query: 541 RAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGNVIHELDKVNNVSKTIFVACEED 600
           RAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGG+VIH+LDKVNNVSKTIFVACEED
Sbjct: 541 RAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGHVIHKLDKVNNVSKTIFVACEED 600

Query: 601 VEEALVAVEKGIWTFNIEWVMTCIMRQEVDLEAPQFAESL 640
           VEEALVAVEKGIWTFNIEW+MTCIMRQEVDLEAPQFAESL
Sbjct: 601 VEEALVAVEKGIWTFNIEWLMTCIMRQEVDLEAPQFAESL 636

BLAST of PI0003250 vs. NCBI nr
Match: XP_008449209.1 (PREDICTED: uncharacterized protein LOC103491160 isoform X2 [Cucumis melo])

HSP 1 Score: 1179.5 bits (3050), Expect = 0.0e+00
Identity = 599/662 (90.48%), Postives = 612/662 (92.45%), Query Frame = 0

Query: 1   MAPRKKPTRRSSIPIGKEGHAVDVNHPECVGKGVGEKCHSQGEYSFVLVNPNDFDGHSKS 60
           MAPRKKPTRRSSI IGKEGHAVDVNH ECVGKGVGEKC SQGEYSFVLVNPNDFD +SKS
Sbjct: 1   MAPRKKPTRRSSIFIGKEGHAVDVNHHECVGKGVGEKCRSQGEYSFVLVNPNDFDSYSKS 60

Query: 61  YLQEVLQLFKRELPTMAYAADTGKQSTFMEKCVSNGKYCTLLLESKSEVDPGLVIAAITY 120
           YLQ+VLQL+KRELPTMAYAA+TGKQSTFMEKC+SNGKYCTLLLESKSEVD GLVIAAITY
Sbjct: 61  YLQDVLQLYKRELPTMAYAANTGKQSTFMEKCLSNGKYCTLLLESKSEVDSGLVIAAITY 120

Query: 121 QIVPADTQYAEIPLAAVCSAYQHK-----------------------GFGHILYMELRKR 180
           QIVPADTQYAEIPLAAV  AYQHK                       GFGHILYMELRKR
Sbjct: 121 QIVPADTQYAEIPLAAVSLAYQHKVSLIASTPSPTGGRVGCSVCLFQGFGHILYMELRKR 180

Query: 181 LQSVGIRTILCWGDKESEGFWSKQGFSSIAEVDTKGKARRIPVRADIRRALCFPGGSTLM 240
           LQSVGIRTI CWGDKESEGFWSKQGF SIAEVDTKGK RRIPVRADIRRALCFPGGSTLM
Sbjct: 181 LQSVGIRTIFCWGDKESEGFWSKQGFLSIAEVDTKGKVRRIPVRADIRRALCFPGGSTLM 240

Query: 241 ISHIQGISMCSADLPKLLSLLKPEAPSSYACAARISVSNRGCNVSNETDQHTIQILNFQP 300
           ISHIQGISMCSADLPKL SLLKPEAP     AARISV+++GCNVSN TDQHTIQILNFQP
Sbjct: 241 ISHIQGISMCSADLPKLRSLLKPEAP----YAARISVASQGCNVSNATDQHTIQILNFQP 300

Query: 301 DEFVTLVPLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCS 360
           DEFVTLVPLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCS
Sbjct: 301 DEFVTLVPLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCS 360

Query: 361 CSTQSAKRVWEASLSSLKSKKVKGVSLDHFHSDSNKKLVPKSNGYDTCIQACSLANSKHE 420
           CS QSAKRVWEASLSSLKSKKVKGV+LDHFHSDSNK LVPKSNGY+ C QACSLANSKHE
Sbjct: 361 CSKQSAKRVWEASLSSLKSKKVKGVNLDHFHSDSNKNLVPKSNGYNACSQACSLANSKHE 420

Query: 421 ILSSIYPKNPTNKYTQNFCQEFRSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLM 480
           ILSSIYPK PTNKYTQNFCQEF SVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLM
Sbjct: 421 ILSSIYPKKPTNKYTQNFCQEFGSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLM 480

Query: 481 KVIEELGGSLTADGCTSTHVITGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDEL 540
           KVIEELGGSLTADG TSTHVITGKVRKTLNFCTALCSGAWIVS SWLKESYREGRFVDEL
Sbjct: 481 KVIEELGGSLTADGSTSTHVITGKVRKTLNFCTALCSGAWIVSSSWLKESYREGRFVDEL 540

Query: 541 PYILNDDDYTSKYRASLKTAVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGN 600
           P ILNDDDYTSKYRASLKT VLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGG+
Sbjct: 541 PCILNDDDYTSKYRASLKTTVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGH 600

Query: 601 VIHELDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWVMTCIMRQEVDLEAPQFAE 640
           VIH+LDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEW+MTCIMRQEVDLEAPQFAE
Sbjct: 601 VIHKLDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWLMTCIMRQEVDLEAPQFAE 658

BLAST of PI0003250 vs. NCBI nr
Match: XP_008449208.1 (PREDICTED: uncharacterized protein LOC103491160 isoform X1 [Cucumis melo])

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 598/663 (90.20%), Postives = 612/663 (92.31%), Query Frame = 0

Query: 1   MAPRKKPTRRSSI-PIGKEGHAVDVNHPECVGKGVGEKCHSQGEYSFVLVNPNDFDGHSK 60
           MAPRKKPTRRSSI  +GKEGHAVDVNH ECVGKGVGEKC SQGEYSFVLVNPNDFD +SK
Sbjct: 1   MAPRKKPTRRSSIFIVGKEGHAVDVNHHECVGKGVGEKCRSQGEYSFVLVNPNDFDSYSK 60

Query: 61  SYLQEVLQLFKRELPTMAYAADTGKQSTFMEKCVSNGKYCTLLLESKSEVDPGLVIAAIT 120
           SYLQ+VLQL+KRELPTMAYAA+TGKQSTFMEKC+SNGKYCTLLLESKSEVD GLVIAAIT
Sbjct: 61  SYLQDVLQLYKRELPTMAYAANTGKQSTFMEKCLSNGKYCTLLLESKSEVDSGLVIAAIT 120

Query: 121 YQIVPADTQYAEIPLAAVCSAYQHK-----------------------GFGHILYMELRK 180
           YQIVPADTQYAEIPLAAV  AYQHK                       GFGHILYMELRK
Sbjct: 121 YQIVPADTQYAEIPLAAVSLAYQHKVSLIASTPSPTGGRVGCSVCLFQGFGHILYMELRK 180

Query: 181 RLQSVGIRTILCWGDKESEGFWSKQGFSSIAEVDTKGKARRIPVRADIRRALCFPGGSTL 240
           RLQSVGIRTI CWGDKESEGFWSKQGF SIAEVDTKGK RRIPVRADIRRALCFPGGSTL
Sbjct: 181 RLQSVGIRTIFCWGDKESEGFWSKQGFLSIAEVDTKGKVRRIPVRADIRRALCFPGGSTL 240

Query: 241 MISHIQGISMCSADLPKLLSLLKPEAPSSYACAARISVSNRGCNVSNETDQHTIQILNFQ 300
           MISHIQGISMCSADLPKL SLLKPEAP     AARISV+++GCNVSN TDQHTIQILNFQ
Sbjct: 241 MISHIQGISMCSADLPKLRSLLKPEAP----YAARISVASQGCNVSNATDQHTIQILNFQ 300

Query: 301 PDEFVTLVPLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHC 360
           PDEFVTLVPLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHC
Sbjct: 301 PDEFVTLVPLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHC 360

Query: 361 SCSTQSAKRVWEASLSSLKSKKVKGVSLDHFHSDSNKKLVPKSNGYDTCIQACSLANSKH 420
           SCS QSAKRVWEASLSSLKSKKVKGV+LDHFHSDSNK LVPKSNGY+ C QACSLANSKH
Sbjct: 361 SCSKQSAKRVWEASLSSLKSKKVKGVNLDHFHSDSNKNLVPKSNGYNACSQACSLANSKH 420

Query: 421 EILSSIYPKNPTNKYTQNFCQEFRSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQL 480
           EILSSIYPK PTNKYTQNFCQEF SVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQL
Sbjct: 421 EILSSIYPKKPTNKYTQNFCQEFGSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQL 480

Query: 481 MKVIEELGGSLTADGCTSTHVITGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDE 540
           MKVIEELGGSLTADG TSTHVITGKVRKTLNFCTALCSGAWIVS SWLKESYREGRFVDE
Sbjct: 481 MKVIEELGGSLTADGSTSTHVITGKVRKTLNFCTALCSGAWIVSSSWLKESYREGRFVDE 540

Query: 541 LPYILNDDDYTSKYRASLKTAVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGG 600
           LP ILNDDDYTSKYRASLKT VLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGG
Sbjct: 541 LPCILNDDDYTSKYRASLKTTVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGG 600

Query: 601 NVIHELDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWVMTCIMRQEVDLEAPQFA 640
           +VIH+LDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEW+MTCIMRQEVDLEAPQFA
Sbjct: 601 HVIHKLDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWLMTCIMRQEVDLEAPQFA 659

BLAST of PI0003250 vs. NCBI nr
Match: XP_038881370.1 (uncharacterized protein LOC120072912 isoform X1 [Benincasa hispida])

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 571/641 (89.08%), Postives = 595/641 (92.82%), Query Frame = 0

Query: 1   MAPRKKPTRRSSIPI-GKEGHAVDVNHPECVGKGVGEKCHSQGEYSFVLVNPNDFDGHSK 60
           MAP+KKPTRRSSIPI GKEG  VDVNHPE  GKG+GEKCHSQGEYSFVLVNPNDFD HSK
Sbjct: 1   MAPKKKPTRRSSIPIVGKEGCVVDVNHPESDGKGIGEKCHSQGEYSFVLVNPNDFDSHSK 60

Query: 61  SYLQEVLQLFKRELPTMAYAADTGKQSTFMEKCVSNGKYCTLLLESKSEVDPGLVIAAIT 120
           SYLQEVLQL+KRELPTM YAA+TGKQSTFM+KCVSNGKYCTLLLESKSEVDPGL+IAAIT
Sbjct: 61  SYLQEVLQLYKRELPTMVYAANTGKQSTFMDKCVSNGKYCTLLLESKSEVDPGLIIAAIT 120

Query: 121 YQIVPADTQYAEIPLAAVCSAYQHKGFGHILYMELRKRLQSVGIRTILCWGDKESEGFWS 180
           YQIVPADTQYAEIPLAAV SAYQHKGFGHILYMELRKRLQSVGIRTI CWGDKESEGFWS
Sbjct: 121 YQIVPADTQYAEIPLAAVSSAYQHKGFGHILYMELRKRLQSVGIRTIFCWGDKESEGFWS 180

Query: 181 KQGFSSIAEVDTKGKARRIPVRADIRRALCFPGGSTLMISHIQGISMCSADLPKLLSLLK 240
           KQGF SIAEVDTKGKARRIPVRADIRRALCFPGGSTLM+SHIQGISMCSADLPK  SLLK
Sbjct: 181 KQGFLSIAEVDTKGKARRIPVRADIRRALCFPGGSTLMVSHIQGISMCSADLPKSFSLLK 240

Query: 241 PEAPSSYACAARISVSNRGCNVSNETDQHTIQILNFQPDEFVTLVPLGEENEIQDPQNQD 300
           PEAPSS   A RI V+N+GCNVSN  DQ T Q LNFQPD+FVTL PLGEEN+IQDPQ QD
Sbjct: 241 PEAPSSCTRAERIGVANQGCNVSNAVDQQTFQNLNFQPDKFVTLAPLGEENKIQDPQIQD 300

Query: 301 AVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCSCSTQSAKRVWEASLSSLKSKKV 360
           AVHD N P SFAEIEN+ TASIAELSNTIGNLDETHCSCSTQSAKRVWEASLSSLKSKKV
Sbjct: 301 AVHDCNSPDSFAEIENHRTASIAELSNTIGNLDETHCSCSTQSAKRVWEASLSSLKSKKV 360

Query: 361 KGVSLDHFHSDSNKKLVPKSNGYDTCIQACSLANSKHEILSSIYPKNP-TNKYTQNFCQE 420
           KGV L HFHSDSNK  VP+SNGYDT  QACSLANSKHEIL+SI  KNP T+ YTQNFC+E
Sbjct: 361 KGVHLPHFHSDSNKNFVPESNGYDTFSQACSLANSKHEILASISLKNPSTSNYTQNFCKE 420

Query: 421 FRSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLMKVIEELGGSLTADGCTSTHVI 480
           F SVNVASE L+CEENTLGKSFKIMLMNIADEAKKTQLMKVIEELGGSLTADG TSTHVI
Sbjct: 421 FGSVNVASEGLDCEENTLGKSFKIMLMNIADEAKKTQLMKVIEELGGSLTADGSTSTHVI 480

Query: 481 TGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDELPYILNDDDYTSKYRASLKTAV 540
           TGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDELPY+LNDDDYTSKYRASLKTAV
Sbjct: 481 TGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDELPYVLNDDDYTSKYRASLKTAV 540

Query: 541 LRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGNVIHELDKVNNVSKTIFVACEE 600
           LRAKARPGALFEGYDVCISAHAQPPPKTLS+IVKSAGGNVIH L KVN VSKTIFVA EE
Sbjct: 541 LRAKARPGALFEGYDVCISAHAQPPPKTLSVIVKSAGGNVIHALGKVNEVSKTIFVAGEE 600

Query: 601 DVEEALVAVEKGIWTFNIEWVMTCIMRQEVDLEAPQFAESL 640
           DVEEAL+AV+KGIWTFN EW+MTC+MRQE+DLEAPQFAESL
Sbjct: 601 DVEEALLAVKKGIWTFNSEWLMTCVMRQELDLEAPQFAESL 641

BLAST of PI0003250 vs. NCBI nr
Match: XP_031740694.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC101209162 [Cucumis sativus])

HSP 1 Score: 1129.4 bits (2920), Expect = 0.0e+00
Identity = 576/655 (87.94%), Postives = 594/655 (90.69%), Query Frame = 0

Query: 1   MAPRKKPTRRSSIPIGKEGHAVDVNHPECVGKGVGEKCHSQGEYSFVLVNPNDFDGHSKS 60
           MAPRKKPT RSSI IGKEGH  DVNH ECVGKGVGEKC SQGEYSFVL+NPNDFD HSKS
Sbjct: 1   MAPRKKPTHRSSILIGKEGH--DVNHHECVGKGVGEKCRSQGEYSFVLINPNDFDSHSKS 60

Query: 61  YLQEVLQLFKRELPTMAYAADTGKQSTFMEKCVSNGKYCTLLLESKSEVDPGLVIAAITY 120
           YLQ+VLQL+KRELPTMAYAA+TGKQSTFMEKCVSNGKYCTLLLESKSEV+PGLVIAAITY
Sbjct: 61  YLQDVLQLYKRELPTMAYAANTGKQSTFMEKCVSNGKYCTLLLESKSEVNPGLVIAAITY 120

Query: 121 QIVPADTQYAEIPLAAVCSAYQHK----------------GFGHILYMELRKRLQSVGIR 180
           QIVPADTQYAEIPLAAV  AYQHK                GFGHILYMELRKRLQSVGIR
Sbjct: 121 QIVPADTQYAEIPLAAVSLAYQHKVSLVPSTPLSHGRKGQGFGHILYMELRKRLQSVGIR 180

Query: 181 TILCWGDKESEGFWSKQGFSSIAEVDTKGKARRIPVRADIRRALCFPGGSTLMISHIQGI 240
           TI CWGDKESEGFWSKQGF SIAEVDTKGK RRIPVRADIRRALCFPGGSTLMISHI+GI
Sbjct: 181 TIFCWGDKESEGFWSKQGFLSIAEVDTKGKVRRIPVRADIRRALCFPGGSTLMISHIKGI 240

Query: 241 SMCSADLPKLLSLLKPEAPSSYACAARISVSNRGCNVSNETDQHTIQILNFQPDEFVTLV 300
           SMCSAD PKL SLLKPEAP     AARISV+NRGCNVSN TDQHTIQ LNFQPDEFVTLV
Sbjct: 241 SMCSADFPKLPSLLKPEAP----YAARISVANRGCNVSNATDQHTIQNLNFQPDEFVTLV 300

Query: 301 PLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCSCSTQSAK 360
           P+GEENEIQ+PQNQDAVHDSNGPVSFAE+ENNTTASIAELSNT+GNLDETHC CS  SAK
Sbjct: 301 PIGEENEIQEPQNQDAVHDSNGPVSFAEVENNTTASIAELSNTLGNLDETHC-CSKHSAK 360

Query: 361 RVWEASLSSLKSKKVKGVSLDHFHSDSNKKLVPKSNGYDTCIQACSLANSKHEILSSIYP 420
           R+WEASLSSLKSKK KGV+LDHFHS SNK L PKS+ YDTC QACSLANSKHEILSSIYP
Sbjct: 361 RIWEASLSSLKSKKKKGVNLDHFHSHSNKNLDPKSDVYDTCSQACSLANSKHEILSSIYP 420

Query: 421 KNPTNKYTQNFCQEFRSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLMKVIEELG 480
           KNPTN+ TQNFCQEF SVNVASEDLN EENTLGKSFKIMLMNIADE KKTQLMKVIEELG
Sbjct: 421 KNPTNQCTQNFCQEFGSVNVASEDLNSEENTLGKSFKIMLMNIADETKKTQLMKVIEELG 480

Query: 481 GSLTADGCTSTHVITGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDELPYILNDD 540
           GSLTADG TSTHV+TGKVRKTLNFC ALCSGAWI S SWLKESYREGRFVDELPYIL+DD
Sbjct: 481 GSLTADGSTSTHVLTGKVRKTLNFCIALCSGAWIFSSSWLKESYREGRFVDELPYILDDD 540

Query: 541 DYTSKYRASLKTAVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGNVIHELDK 600
           DY SKYRASLK AVLRAKA  GALFEGYDVCISAHAQPPPKTLSLIVKSAGGNVIHELDK
Sbjct: 541 DYISKYRASLKAAVLRAKACSGALFEGYDVCISAHAQPPPKTLSLIVKSAGGNVIHELDK 600

Query: 601 VNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWVMTCIMRQEVDLEAPQFAESL 640
           VNNV KTIFVACEEDVEEALVAVEKGIWTFN EW+M CIMRQEVDLEAPQFAESL
Sbjct: 601 VNNVWKTIFVACEEDVEEALVAVEKGIWTFNAEWLMACIMRQEVDLEAPQFAESL 648

BLAST of PI0003250 vs. TAIR 10
Match: AT2G41450.1 (N-acetyltransferases;N-acetyltransferases )

HSP 1 Score: 506.9 bits (1304), Expect = 2.4e-143
Identity = 294/659 (44.61%), Postives = 401/659 (60.85%), Query Frame = 0

Query: 1   MAP-RKKPTRRSSIPIGKEGHAVD--------------VNHPECVGKGVGEKCHSQGEY- 60
           MAP R  P + S   IG    AVD              +  P      + EK     +Y 
Sbjct: 1   MAPKRASPMQSSLYSIGDYEVAVDGKNFTCESGPKSIQIKFPRNYRVKISEKDKIVSDYP 60

Query: 61  ---SFVLVNPNDFDGHSKSYLQEVLQLFKRELPTMAYAADTGKQSTFMEKCVSNGKYCTL 120
               F+L+NP D D  +KS+LQEVL+L+ +ELP M YA++TGKQS F+E+CVS GKYC+L
Sbjct: 61  KEDMFLLLNPRDEDDFTKSHLQEVLKLYSKELPDMRYASNTGKQSAFLERCVSKGKYCSL 120

Query: 121 LLESKSEVDPGLVIAAITYQIVPADTQYAEIPLAAVCSAYQHKGFGHILYMELRKRLQSV 180
           +L+S    D   ++AAITYQIVPADTQYAEIPLAAV   +Q KGFG ++Y EL KRL SV
Sbjct: 121 VLKSTLGGDSDEILAAITYQIVPADTQYAEIPLAAVTYTHQKKGFGKLVYEELMKRLHSV 180

Query: 181 GIRTILCWGDKESEGFWSKQGFSSIAEVDTKGKARRIPVRADIRRALCFPGGSTLMISHI 240
           GIRTI CW DKESEGFW KQGF  +AEVD KGKA+ + ++++IR+ALCFPGGSTLM+SH+
Sbjct: 181 GIRTIYCWADKESEGFWLKQGFIKLAEVDHKGKAKGLHIKSNIRKALCFPGGSTLMLSHL 240

Query: 241 QGISMCSADLPKLLSLLKPEAPSSYAC-AARISVSNRGCNVSNETDQHTIQILNFQPDEF 300
                      + +S L+  +   Y C  + +S  N      N T   T   +  + + F
Sbjct: 241 N---------KEFISNLEISSSWKYQCEGSPLSARN------NSTGPVTGDSVKLR-ESF 300

Query: 301 VTLVPLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCSCST 360
              V L   + I+ P +     ++N  +S    +  TTA             ++   CST
Sbjct: 301 GESVYLDCISGIRSPMDSITGKENNNVIS----DQATTA-------------DSETKCST 360

Query: 361 QSAKRVWEASLSSLKSKKVKGVSLDHFHSDSNKKLVPKSNGYDTCIQACSLANSKHEILS 420
              KR WEASLSSL+SK+++       ++++N ++        +  Q+    +S+ +I  
Sbjct: 361 PGLKRSWEASLSSLQSKRIRA------NNNNNSEIAKTDLAQSSAKQSKDGNSSQVDITK 420

Query: 421 SIYPKNPTNKYTQNFCQEFRSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLMKVI 480
              P        +N  ++ R     +  ++ E    G+ ++I+LM+I DE K+  L +VI
Sbjct: 421 DSLP----TICKRNDVEQCR----MATGIDMEARPNGQHYRILLMDICDENKRAWLTEVI 480

Query: 481 EELGGSLTADGCTSTHVITGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDELPYI 540
            +LGG++T DG TSTH++TGKVRKTLN CTALCSGAWIVSPSWLKES REGRF +E  +I
Sbjct: 481 RKLGGTVTLDGTTSTHIVTGKVRKTLNLCTALCSGAWIVSPSWLKESVREGRFANEASHI 540

Query: 541 LNDDDYTSKYRASLKTAVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGNVIH 600
           L+D+DY  KY   LK+ VLRAKARP +L +GYD+C+  + + P KT S I+KSAGGNVI 
Sbjct: 541 LHDEDYQLKYDTDLKSTVLRAKARPNSLLKGYDICVGPNIELPIKTSSAIIKSAGGNVIS 600

Query: 601 ELDKVNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWVMTCIMRQEVDLEAPQFAESL 640
            ++KV   SK I++ CEED   AL A +KGIWTF+ EW M C+M+Q++DL+ PQF ESL
Sbjct: 601 GVNKVKEASKAIYIGCEEDTVGALFAAKKGIWTFSSEWFMNCVMKQQLDLQVPQFVESL 612

BLAST of PI0003250 vs. TAIR 10
Match: AT2G41450.2 (N-acetyltransferases;N-acetyltransferases )

HSP 1 Score: 506.9 bits (1304), Expect = 2.4e-143
Identity = 279/595 (46.89%), Postives = 382/595 (64.20%), Query Frame = 0

Query: 46  FVLVNPNDFDGHSKSYLQEVLQLFKRELPTMAYAADTGKQSTFMEKCVSNGKYCTLLLES 105
           F+L+NP D D  +KS+LQEVL+L+ +ELP M YA++TGKQS F+E+CVS GKYC+L+L+S
Sbjct: 2   FLLLNPRDEDDFTKSHLQEVLKLYSKELPDMRYASNTGKQSAFLERCVSKGKYCSLVLKS 61

Query: 106 KSEVDPGLVIAAITYQIVPADTQYAEIPLAAVCSAYQHKGFGHILYMELRKRLQSVGIRT 165
               D   ++AAITYQIVPADTQYAEIPLAAV   +Q KGFG ++Y EL KRL SVGIRT
Sbjct: 62  TLGGDSDEILAAITYQIVPADTQYAEIPLAAVTYTHQKKGFGKLVYEELMKRLHSVGIRT 121

Query: 166 ILCWGDKESEGFWSKQGFSSIAEVDTKGKARRIPVRADIRRALCFPGGSTLMISHIQGIS 225
           I CW DKESEGFW KQGF  +AEVD KGKA+ + ++++IR+ALCFPGGSTLM+SH+    
Sbjct: 122 IYCWADKESEGFWLKQGFIKLAEVDHKGKAKGLHIKSNIRKALCFPGGSTLMLSHLN--- 181

Query: 226 MCSADLPKLLSLLKPEAPSSYAC-AARISVSNRGCNVSNETDQHTIQILNFQPDEFVTLV 285
                  + +S L+  +   Y C  + +S  N      N T   T   +  + + F   V
Sbjct: 182 ------KEFISNLEISSSWKYQCEGSPLSARN------NSTGPVTGDSVKLR-ESFGESV 241

Query: 286 PLGEENEIQDPQNQDAVHDSNGPVSFAEIENNTTASIAELSNTIGNLDETHCSCSTQSAK 345
            L   + I+ P +     ++N  +S    +  TTA             ++   CST   K
Sbjct: 242 YLDCISGIRSPMDSITGKENNNVIS----DQATTA-------------DSETKCSTPGLK 301

Query: 346 RVWEASLSSLKSKKVKGVSLDHFHSDSNKKLVPKSNGYDTCIQACSLANSKHEILSSIYP 405
           R WEASLSSL+SK+++       ++++N ++        +  Q+    +S+ +I     P
Sbjct: 302 RSWEASLSSLQSKRIRA------NNNNNSEIAKTDLAQSSAKQSKDGNSSQVDITKDSLP 361

Query: 406 KNPTNKYTQNFCQEFRSVNVASEDLNCEENTLGKSFKIMLMNIADEAKKTQLMKVIEELG 465
                   +N  ++ R     +  ++ E    G+ ++I+LM+I DE K+  L +VI +LG
Sbjct: 362 ----TICKRNDVEQCR----MATGIDMEARPNGQHYRILLMDICDENKRAWLTEVIRKLG 421

Query: 466 GSLTADGCTSTHVITGKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDELPYILNDD 525
           G++T DG TSTH++TGKVRKTLN CTALCSGAWIVSPSWLKES REGRF +E  +IL+D+
Sbjct: 422 GTVTLDGTTSTHIVTGKVRKTLNLCTALCSGAWIVSPSWLKESVREGRFANEASHILHDE 481

Query: 526 DYTSKYRASLKTAVLRAKARPGALFEGYDVCISAHAQPPPKTLSLIVKSAGGNVIHELDK 585
           DY  KY   LK+ VLRAKARP +L +GYD+C+  + + P KT S I+KSAGGNVI  ++K
Sbjct: 482 DYQLKYDTDLKSTVLRAKARPNSLLKGYDICVGPNIELPIKTSSAIIKSAGGNVISGVNK 541

Query: 586 VNNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWVMTCIMRQEVDLEAPQFAESL 640
           V   SK I++ CEED   AL A +KGIWTF+ EW M C+M+Q++DL+ PQF ESL
Sbjct: 542 VKEASKAIYIGCEEDTVGALFAAKKGIWTFSSEWFMNCVMKQQLDLQVPQFVESL 549

BLAST of PI0003250 vs. TAIR 10
Match: AT4G03130.1 (BRCT domain-containing DNA repair protein )

HSP 1 Score: 80.9 bits (198), Expect = 4.2e-15
Identity = 59/196 (30.10%), Postives = 95/196 (48.47%), Query Frame = 0

Query: 446 NIADEAKKTQLMKVIEELGGSLTADGCTSTHVITGKVRKTLNFCTALCSGAWIVSPSWLK 505
           N+ DE  K Q  K++  LG S  +    STH I  +  +T N   A+  G ++V+P WL+
Sbjct: 568 NLDDETVKQQ-KKIMVRLGISPASSSADSTHFIADRFARTRNMLEAIALGKFVVTPIWLE 627

Query: 506 ESYREGRFVDELPYILNDDDYTSKYRASLKTAVLRAKARPGALFEGYDVCISAHAQPPPK 565
              +    +DE  YIL D     K    L T++ RAK  P  L +G+ VCI+   +P   
Sbjct: 628 SCAQTRCLIDEKSYILRDIK-KEKDGFCLLTSLARAKQHP--LLKGFKVCITPSIKPSRG 687

Query: 566 TLSLIVKSAGGNVIHELDKV-----NNVSKTIFVACEEDVEEALVAVEKGIWTFNIEWVM 625
            ++ +VK   G V+   + +     N     + ++C+ED +  L  V +G   F  E ++
Sbjct: 688 MITDLVKMTQGQVVEASEIIAAEDRNFPEDVLILSCKEDRDFCLPFVNQGAVIFTSELLL 747

Query: 626 TCIMRQEVDLEAPQFA 637
             I+ Q+  LE  +FA
Sbjct: 748 NGIVIQK--LEYARFA 757

BLAST of PI0003250 vs. TAIR 10
Match: AT1G04020.2 (breast cancer associated RING 1 )

HSP 1 Score: 54.7 bits (130), Expect = 3.2e-07
Identity = 40/126 (31.75%), Postives = 60/126 (47.62%), Query Frame = 0

Query: 475 THVIT-----GKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDELPYILNDDDYTSK 534
           THVI      G   +TL     + +G WI++ +W+K S +  + VDE P+ +  D  T  
Sbjct: 525 THVIASTDEKGACTRTLKVLMGILNGKWIINAAWMKASLKASQPVDEEPFEIQID--TQG 584

Query: 535 YRASLKTAVLRAKARPGALFEGYDVCISAHAQPPPK-TLSLIVKSAGGNVIHELDKV--- 590
            +   KTA LRA+     LFEG             K  L  +VK AGG +++  D++   
Sbjct: 585 CQDGPKTARLRAETNKPKLFEGLKFYFFGDFYKGYKEDLQNLVKVAGGTILNTEDELGAE 644

BLAST of PI0003250 vs. TAIR 10
Match: AT1G04020.1 (breast cancer associated RING 1 )

HSP 1 Score: 54.7 bits (130), Expect = 3.2e-07
Identity = 40/126 (31.75%), Postives = 60/126 (47.62%), Query Frame = 0

Query: 475 THVIT-----GKVRKTLNFCTALCSGAWIVSPSWLKESYREGRFVDELPYILNDDDYTSK 534
           THVI      G   +TL     + +G WI++ +W+K S +  + VDE P+ +  D  T  
Sbjct: 526 THVIASTDEKGACTRTLKVLMGILNGKWIINAAWMKASLKASQPVDEEPFEIQID--TQG 585

Query: 535 YRASLKTAVLRAKARPGALFEGYDVCISAHAQPPPK-TLSLIVKSAGGNVIHELDKV--- 590
            +   KTA LRA+     LFEG             K  L  +VK AGG +++  D++   
Sbjct: 586 CQDGPKTARLRAETNKPKLFEGLKFYFFGDFYKGYKEDLQNLVKVAGGTILNTEDELGAE 645