PI0003193 (gene) Melon (PI 482460) v1

Overview
NamePI0003193
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionSulfate adenylyltransferase
Locationchr04: 29558522 .. 29561532 (-)
RNA-Seq ExpressionPI0003193
SyntenyPI0003193
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACATTGACAACCACAGCCACAAAAGTGTTTTTAGTCTCAAACGAAGCGAGAGAGAGATCCGATTAGCCGCAACCAAACGCATCTTCCCCTTCTCTAAGCACAAAAGGCCACAAACAAACGTGTGGATCTCTTAAGACTACACAATTTTCTTGGTTCCCTTACAAGCATGTTCATCTTTTCCATTTTTACAGATACATCTAACCCATTACTCCATTTTCATCGGACCTCTATATTCCTATTTTGGCAAATACCAAAACCCATTTCCCCATTTTTCTTTCTCAACCCAATATGTCTCTATTCACTAAACTCAACATCGCCATTTCTCCTCAATTCACCATCAACAACCTTCAAACTACAATAAAAACAACATCCATTCCCATTTACAACTCCAAAATTCAATCGAAACACATTTACGTTTCTCACCCTTTGGTGTTGTTTAAACCCACAATGCAGGGTCATTCACCTCGTTCTCTTCGCTCCTCGATCAAGAGCTCGTTGATAGACCCCGATGGTGGGGTTGTCGTCGACTTGGTTGTGCCTGAGAGTGAAAGGGCTTCGAGGGTTTTGGAAGCCGAGCCGTTGCCGAAGGTTAAATTGACCCAAATTGATCTAGAATGGGTCCACGTCATTAGTGAAGGATGGGCGAGCCCGTTGAAAGGGTACATGAGAGAAGATGAGTATTTGCAGAGTTTGCATTTCAATTGCATCAAAATTAAAGATGGGTCTTTCGTAAACATGTCGCTTCCTATTGTTCTTGCCATTGGCGACGATGTTAAGGACCAAATTGGGTCCTCATCTAATGTGGGGTTGCTTGGTCCCAATGGAGATTTGGTTGGTATTCTTCGCAGGTTAGTTTTTTCTGAAGAAGGGTTAGTTAATTTAATTTCATTCGATCTTTTAGCCTCTATATATGAAGATATGGAGATGGGGTTTTTTGGATTTTTGTTTGAAGTATCGATTTATGAAATATGAGTTGTTTTCTTTTCCACCTTATGTTTGGCCACCTATAATTGAAGTTATGATATGACACGAGTTAAATTATTTAAATCCTAAGAGATTCTCTGTTACAATTATAATATTTATAATATTCCACTTGCTTGAAACAACTGTGTTGATCATGTCATATTTTATGTTGGAAAACAATATTTTGATAATGCTCTACAATCTTGTTTGTAACAAGATCTCTGTTTATGTGAAGTTCAAACAAAGATGACAATGGTGATGATCTTAGATTTCTGGGTTTACAAATGCAAACGCCTAATGTTGGACATCTTTTAGAAACTTTCTTTTTGTGAATAGTGAGGCCAATATGTACAGAAATATGTAGTCAAACTGAAACCAAATTATGTGATTTTCCATCCTCACTTTGAATAATTTTGCAGCATTGAGATATTCAAGCATAATAAGGAAGAGAGGATAGCTAGAACTTGGGGCACAACTGCACCTGGGTTACCATATGTTGATGAGGTCATTACAAATGCTGGGAACTGGCTTCTTGGTGGAGATCTTGAAGTAATAAACCCCATCAAATACAATGATGGACTTGATCATTATAGGCTGTCTCCGAAACAACTTCGGCAAGAGTTCGATAGGCGTCAGGCCGATGCTGTTTTTGCCTTTCAATTGAGAAACCCTGTACATAATGGTCATGCTTTGTTAATGAATGATACACGAAAGCGACTTCTAGATATGGGTTACAAGAATCCAATTCTATTGCTTCATCCTTTGGGAGGTTTCACAAAAGCAGATGATGTGCCATTGGATGTTCGAATGGAGCAACATAGCAAGGTTTGTTTTCCATTATCATCATTCTAAATAGTTATTTTCTTTATCATTCAAAAACTTCCCCATCTTCATACTCTTTACTCTTTAGAAGTGAAAATGTACAGTTGTCTAACTTTTCCAACGTTCAATAACTTGAAATGAAAGATTTGATTTTCTCGAGCAAAGAAGTCAATAGAATGCTAGTTGAATGAGATGATTCCTCTCTGCTCTTCAGTTAATATGAGTGTTCAATTTTCTGGGTTGTTTACTTAATTTCTGTTATTTATGAGAGACTTTCTGTGTCTATAGGTTTTAGAAGATGGAGTCCTTGACCCTGAAACAACCATCGTCTCTATTTTCCCGTCACCAATGCATTACGCTGGTCCAACCGAAGTACAATGGCATGCTAAAGCAAGGATCAATGCAGGTGCAAATTTTTACATTGTTGGTCGAGATCCTGCTGGAATGGGTCACCCAACAGAGAAGAGAGATTTATATGATCCAGACCATGGCAAGAAGGTCTTAAGTATGGCCCCTGGCCTTGAGAAGCTAAACATATTGCCCTTCAAGGTCAATATCTCAATGACCCTTCCTTCTCCTTTCCCTACTTTCCACCCTCTTCATTTTAACATCCAGTTGACCGCAGGTAGCAGCATACGATACTGTGGCGAAGAAGATGGCATTCTTCGACCCTTCTCGTGCTAAAGATTTCCTCTTCATCTCTGGAACAAAGGTAAGAGCTAAGTTGAGTTTGGACAAGATGTCATTTTAATCCTGCTACGACAACAGTAACTGAGTTTTACATGTGGTTTGTGTTAGATGCGAACATACGCAAGGACCGGTGAGAATCCTCCTGACGGTTTCATGTGCCCTGGCGGATGGAAGGTGTTGGTCAATTATTACGAGAGCTTGCAGGCGGAAGAGGCATCGTCGCAGCAGGCTGCATTGTCAGTTTAGGGAGGAAATTTTGAGGTCATTTGAGAAGGAATGAATTGTAGTAAATCTATGCGAGTTGATTGAGGAAGGTGCCGAGGATTGATTTGGCAGAATGCCAACTTCGATTGTGTATAATTTTATATGTATTCTCGGTTTTTATGCTTTTTAGATTTTATTTTTGTTCAAATGTCTTTTAGATATGCTTGTGAAAGTGAATTATTTGTCGCTTCTCTTTACTTGTTAAATGATATTCTTATATTTTGGATCCTCTTTAATTTATTTATATATGCAGTTGATGGTTATAGTAGAAA

mRNA sequence

ACATTGACAACCACAGCCACAAAAGTGTTTTTAGTCTCAAACGAAGCGAGAGAGAGATCCGATTAGCCGCAACCAAACGCATCTTCCCCTTCTCTAAGCACAAAAGGCCACAAACAAACGTGTGGATCTCTTAAGACTACACAATTTTCTTGGTTCCCTTACAAGCATGTTCATCTTTTCCATTTTTACAGATACATCTAACCCATTACTCCATTTTCATCGGACCTCTATATTCCTATTTTGGCAAATACCAAAACCCATTTCCCCATTTTTCTTTCTCAACCCAATATGTCTCTATTCACTAAACTCAACATCGCCATTTCTCCTCAATTCACCATCAACAACCTTCAAACTACAATAAAAACAACATCCATTCCCATTTACAACTCCAAAATTCAATCGAAACACATTTACGTTTCTCACCCTTTGGTGTTGTTTAAACCCACAATGCAGGGTCATTCACCTCGTTCTCTTCGCTCCTCGATCAAGAGCTCGTTGATAGACCCCGATGGTGGGGTTGTCGTCGACTTGGTTGTGCCTGAGAGTGAAAGGGCTTCGAGGGTTTTGGAAGCCGAGCCGTTGCCGAAGGTTAAATTGACCCAAATTGATCTAGAATGGGTCCACGTCATTAGTGAAGGATGGGCGAGCCCGTTGAAAGGGTACATGAGAGAAGATGAGTATTTGCAGAGTTTGCATTTCAATTGCATCAAAATTAAAGATGGGTCTTTCGTAAACATGTCGCTTCCTATTGTTCTTGCCATTGGCGACGATGTTAAGGACCAAATTGGGTCCTCATCTAATGTGGGGTTGCTTGGTCCCAATGGAGATTTGGTTGGTATTCTTCGCAGCATTGAGATATTCAAGCATAATAAGGAAGAGAGGATAGCTAGAACTTGGGGCACAACTGCACCTGGGTTACCATATGTTGATGAGGTCATTACAAATGCTGGGAACTGGCTTCTTGGTGGAGATCTTGAAGTAATAAACCCCATCAAATACAATGATGGACTTGATCATTATAGGCTGTCTCCGAAACAACTTCGGCAAGAGTTCGATAGGCGTCAGGCCGATGCTGTTTTTGCCTTTCAATTGAGAAACCCTGTACATAATGGTCATGCTTTGTTAATGAATGATACACGAAAGCGACTTCTAGATATGGGTTACAAGAATCCAATTCTATTGCTTCATCCTTTGGGAGGTTTCACAAAAGCAGATGATGTGCCATTGGATGTTCGAATGGAGCAACATAGCAAGGTTTTAGAAGATGGAGTCCTTGACCCTGAAACAACCATCGTCTCTATTTTCCCGTCACCAATGCATTACGCTGGTCCAACCGAAGTACAATGGCATGCTAAAGCAAGGATCAATGCAGGTGCAAATTTTTACATTGTTGGTCGAGATCCTGCTGGAATGGGTCACCCAACAGAGAAGAGAGATTTATATGATCCAGACCATGGCAAGAAGGTCTTAAGTATGGCCCCTGGCCTTGAGAAGCTAAACATATTGCCCTTCAAGGTAGCAGCATACGATACTGTGGCGAAGAAGATGGCATTCTTCGACCCTTCTCGTGCTAAAGATTTCCTCTTCATCTCTGGAACAAAGATGCGAACATACGCAAGGACCGGTGAGAATCCTCCTGACGGTTTCATGTGCCCTGGCGGATGGAAGGTGTTGGTCAATTATTACGAGAGCTTGCAGGCGGAAGAGGCATCGTCGCAGCAGGCTGCATTGTCAGTTTAGGGAGGAAATTTTGAGGTCATTTGAGAAGGAATGAATTGTAGTAAATCTATGCGAGTTGATTGAGGAAGGTGCCGAGGATTGATTTGGCAGAATGCCAACTTCGATTGTGTATAATTTTATATGTATTCTCGGTTTTTATGCTTTTTAGATTTTATTTTTGTTCAAATGTCTTTTAGATATGCTTGTGAAAGTGAATTATTTGTCGCTTCTCTTTACTTGTTAAATGATATTCTTATATTTTGGATCCTCTTTAATTTATTTATATATGCAGTTGATGGTTATAGTAGAAA

Coding sequence (CDS)

ATGTCTCTATTCACTAAACTCAACATCGCCATTTCTCCTCAATTCACCATCAACAACCTTCAAACTACAATAAAAACAACATCCATTCCCATTTACAACTCCAAAATTCAATCGAAACACATTTACGTTTCTCACCCTTTGGTGTTGTTTAAACCCACAATGCAGGGTCATTCACCTCGTTCTCTTCGCTCCTCGATCAAGAGCTCGTTGATAGACCCCGATGGTGGGGTTGTCGTCGACTTGGTTGTGCCTGAGAGTGAAAGGGCTTCGAGGGTTTTGGAAGCCGAGCCGTTGCCGAAGGTTAAATTGACCCAAATTGATCTAGAATGGGTCCACGTCATTAGTGAAGGATGGGCGAGCCCGTTGAAAGGGTACATGAGAGAAGATGAGTATTTGCAGAGTTTGCATTTCAATTGCATCAAAATTAAAGATGGGTCTTTCGTAAACATGTCGCTTCCTATTGTTCTTGCCATTGGCGACGATGTTAAGGACCAAATTGGGTCCTCATCTAATGTGGGGTTGCTTGGTCCCAATGGAGATTTGGTTGGTATTCTTCGCAGCATTGAGATATTCAAGCATAATAAGGAAGAGAGGATAGCTAGAACTTGGGGCACAACTGCACCTGGGTTACCATATGTTGATGAGGTCATTACAAATGCTGGGAACTGGCTTCTTGGTGGAGATCTTGAAGTAATAAACCCCATCAAATACAATGATGGACTTGATCATTATAGGCTGTCTCCGAAACAACTTCGGCAAGAGTTCGATAGGCGTCAGGCCGATGCTGTTTTTGCCTTTCAATTGAGAAACCCTGTACATAATGGTCATGCTTTGTTAATGAATGATACACGAAAGCGACTTCTAGATATGGGTTACAAGAATCCAATTCTATTGCTTCATCCTTTGGGAGGTTTCACAAAAGCAGATGATGTGCCATTGGATGTTCGAATGGAGCAACATAGCAAGGTTTTAGAAGATGGAGTCCTTGACCCTGAAACAACCATCGTCTCTATTTTCCCGTCACCAATGCATTACGCTGGTCCAACCGAAGTACAATGGCATGCTAAAGCAAGGATCAATGCAGGTGCAAATTTTTACATTGTTGGTCGAGATCCTGCTGGAATGGGTCACCCAACAGAGAAGAGAGATTTATATGATCCAGACCATGGCAAGAAGGTCTTAAGTATGGCCCCTGGCCTTGAGAAGCTAAACATATTGCCCTTCAAGGTAGCAGCATACGATACTGTGGCGAAGAAGATGGCATTCTTCGACCCTTCTCGTGCTAAAGATTTCCTCTTCATCTCTGGAACAAAGATGCGAACATACGCAAGGACCGGTGAGAATCCTCCTGACGGTTTCATGTGCCCTGGCGGATGGAAGGTGTTGGTCAATTATTACGAGAGCTTGCAGGCGGAAGAGGCATCGTCGCAGCAGGCTGCATTGTCAGTTTAG

Protein sequence

MSLFTKLNIAISPQFTINNLQTTIKTTSIPIYNSKIQSKHIYVSHPLVLFKPTMQGHSPRSLRSSIKSSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGWASPLKGYMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKMAFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASSQQAALSV
Homology
BLAST of PI0003193 vs. ExPASy Swiss-Prot
Match: Q43870 (ATP sulfurylase 2 OS=Arabidopsis thaliana OX=3702 GN=APS2 PE=1 SV=1)

HSP 1 Score: 756.1 bits (1951), Expect = 2.4e-217
Identity = 358/430 (83.26%), Postives = 393/430 (91.40%), Query Frame = 0

Query: 53  TMQGHSPRSLRSSIKSSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVH 112
           TMQ  S    + ++KSSLIDPDGG +V+L+VPE+E   +  E+E +PKVKL QIDLEWVH
Sbjct: 51  TMQSVS----KMTVKSSLIDPDGGELVELIVPETEIGVKKAESETMPKVKLNQIDLEWVH 110

Query: 113 VISEGWASPLKGYMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNV 172
           VISEGWASPLKG+MREDEYLQSLHFN +++K+G+FVNMSLPIVLAI DD K+QIGSS NV
Sbjct: 111 VISEGWASPLKGFMREDEYLQSLHFNSLRLKNGTFVNMSLPIVLAIDDDTKEQIGSSENV 170

Query: 173 GLLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVI 232
            L+ P GD++G LRS+EI+KHNKEERIARTWGTT+PGLPYV+E IT +GNWL+GGDLEV 
Sbjct: 171 ALVCPQGDIIGSLRSVEIYKHNKEERIARTWGTTSPGLPYVEEYITPSGNWLIGGDLEVF 230

Query: 233 NPIKYNDGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGY 292
            PIKYNDGLDHYRLSPKQLR+EFD RQADAVFAFQLRNPVHNGHALLMNDTRKRLL+MGY
Sbjct: 231 EPIKYNDGLDHYRLSPKQLREEFDNRQADAVFAFQLRNPVHNGHALLMNDTRKRLLEMGY 290

Query: 293 KNPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQ 352
           KNP+LLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDP+TTIVSIFPSPMHYAGPTEVQ
Sbjct: 291 KNPVLLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPKTTIVSIFPSPMHYAGPTEVQ 350

Query: 353 WHAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAA 412
           WHAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGK+VLSMAPGLEKLNILPF+VAA
Sbjct: 351 WHAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKRVLSMAPGLEKLNILPFRVAA 410

Query: 413 YDTVAKKMAFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAE 472
           YDT+ KKMAFFDPSRAK+FLFISGTKMRTYARTGENPPDGFMCP GW VLV YYESLQ  
Sbjct: 411 YDTIEKKMAFFDPSRAKEFLFISGTKMRTYARTGENPPDGFMCPSGWNVLVKYYESLQES 470

Query: 473 EASSQQAALS 483
           EA  QQA +S
Sbjct: 471 EA-KQQAVVS 475

BLAST of PI0003193 vs. ExPASy Swiss-Prot
Match: Q9LIK9 (ATP sulfurylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS1 PE=1 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 1.2e-192
Identity = 322/443 (72.69%), Postives = 368/443 (83.07%), Query Frame = 0

Query: 36  IQSKHIYVSHPLVLFKPTMQ------GHSPRSLR---SSIKSSLIDPDGGVVVDLVVPES 95
           + SK  ++S PL    P             +SLR    SI++ LI PDGG +V+L+V E 
Sbjct: 7   VLSKTPFLSQPLTKSSPNSDLPFAAVSFPSKSLRRRVGSIRAGLIAPDGGKLVELIVEEP 66

Query: 96  ERASRVLEAEPLPKVKLTQIDLEWVHVISEGWASPLKGYMREDEYLQSLHFNCIKIKDGS 155
           +R  +  EA  LP+V+LT IDL+W+HV+SEGWASPL G+MRE E+LQ+LHFN +++ DGS
Sbjct: 67  KRREKKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTLHFNSLRLDDGS 126

Query: 156 FVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGDLVGILRSIEIFKHNKEERIARTWGTT 215
            VNMS+PIVLAI D+ K +IG S+ V L   +G+ V IL  IEI+KH KEERIARTWGTT
Sbjct: 127 VVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIEIYKHPKEERIARTWGTT 186

Query: 216 APGLPYVDEVITNAGNWLLGGDLEVINPIKYNDGLDHYRLSPKQLRQEFDRRQADAVFAF 275
           APGLPYVDE ITNAGNWL+GGDLEV+ P+KYNDGLD +RLSP +LR+E ++R ADAVFAF
Sbjct: 187 APGLPYVDEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSPAELRKELEKRNADAVFAF 246

Query: 276 QLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPLGGFTKADDVPLDVRMEQHSKVLED 335
           QLRNPVHNGHALLM DTR+RLL+MGYKNPILLLHPLGGFTKADDVPLD RM+QH KVLED
Sbjct: 247 QLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLDWRMKQHEKVLED 306

Query: 336 GVLDPETTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDLYD 395
           GVLDPETT+VSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP EKRDLYD
Sbjct: 307 GVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPVEKRDLYD 366

Query: 396 PDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKMAFFDPSRAKDFLFISGTKMRTYARTG 455
            DHGKKVLSMAPGLE+LNILPF+VAAYD    KMAFFDPSR +DFLFISGTKMRT A+  
Sbjct: 367 ADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTKMRTLAKNN 426

Query: 456 ENPPDGFMCPGGWKVLVNYYESL 470
           ENPPDGFMCPGGWKVLV+YYESL
Sbjct: 427 ENPPDGFMCPGGWKVLVDYYESL 449

BLAST of PI0003193 vs. ExPASy Swiss-Prot
Match: O23324 (ATP-sulfurylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS3 PE=1 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 2.1e-189
Identity = 312/411 (75.91%), Postives = 356/411 (86.62%), Query Frame = 0

Query: 60  RSLRS-SIKSSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGW 119
           RSLR+ S+++ LI+PDGG +VDLVVPE  R  +  EA  LP+V+LT IDL+W+HV+SEGW
Sbjct: 41  RSLRTISVRAGLIEPDGGKLVDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEGW 100

Query: 120 ASPLKGYMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPN 179
           ASPL+G+MRE E+LQ+LHFN + + DGS VNMS+PIVLAI D  K  IG S  V L+  +
Sbjct: 101 ASPLRGFMRESEFLQTLHFNLLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSD 160

Query: 180 GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYN 239
            + + IL  IEI+KH KEERIARTWGTTAPGLPYV+E ITNAG+WL+GGDLEV+ P+KYN
Sbjct: 161 DNPIAILNDIEIYKHPKEERIARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYN 220

Query: 240 DGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL 299
           DGLD +RLSP +LR+E ++R ADAVFAFQLRNPVHNGHALLM DTR+RLL+MGYKNPILL
Sbjct: 221 DGLDRFRLSPFELRKELEKRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILL 280

Query: 300 LHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKAR 359
           LHPLGGFTKADDVPL  RM+QH KVLEDGVLDPETT+VSIFPSPM YAGPTEVQWHAKAR
Sbjct: 281 LHPLGGFTKADDVPLSWRMKQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKAR 340

Query: 360 INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAK 419
           INAGANFYIVGRDPAGMGHP EKRDLYD DHGKKVLSMAPGLE+LNILPF+VAAYD    
Sbjct: 341 INAGANFYIVGRDPAGMGHPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQG 400

Query: 420 KMAFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESL 470
           KMAFFDPSRA+DFLFISGTKMR  A+  ENPPDGFMCPGGWKVLV+YY+SL
Sbjct: 401 KMAFFDPSRAQDFLFISGTKMRALAKNRENPPDGFMCPGGWKVLVDYYDSL 451

BLAST of PI0003193 vs. ExPASy Swiss-Prot
Match: Q9S7D8 (ATP sulfurylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS4 PE=2 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 4.4e-187
Identity = 311/414 (75.12%), Postives = 355/414 (85.75%), Query Frame = 0

Query: 65  SIKSSLIDPDGGVVVDLVVPESERASRVLEAEPLP-KVKLTQIDLEWVHVISEGWASPLK 124
           S+KS LI+PDGG +++LVV ES R     EAE +P ++KL ++DLEWVHV+SEGWASPLK
Sbjct: 48  SVKSGLIEPDGGKLMNLVVEESRRRVMKHEAETVPARIKLNRVDLEWVHVLSEGWASPLK 107

Query: 125 GYMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGDLVG 184
           G+MR+ E+LQ+LHFN  +++DGS VNMS+PIVLAI DD K +IG S+ V L+   G+ + 
Sbjct: 108 GFMRQSEFLQTLHFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIA 167

Query: 185 ILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDGLDH 244
           IL  IEI+KH KEERIARTWGTTA GLPY +E IT AGNWL+GGDL+V+ PIKYNDGLD 
Sbjct: 168 ILNDIEIYKHPKEERIARTWGTTARGLPYAEEAITKAGNWLIGGDLQVLEPIKYNDGLDR 227

Query: 245 YRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPLG 304
           +RLSP QLR+EF RR ADAVFAFQLRNPVHNGHALLM DTR+RLL+MGYKNP+LLL+PLG
Sbjct: 228 FRLSPSQLREEFIRRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLNPLG 287

Query: 305 GFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARINAGA 364
           GFTKADDVPL  RM QH KVLEDGVLDPETT+VSIFPSPM YAGPTEVQWHAKARINAGA
Sbjct: 288 GFTKADDVPLSWRMRQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGA 347

Query: 365 NFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKMAFF 424
           NFYIVGRDPAGMGHPTEKRDLYD DHGKKVLSMAPGLE+LNILPFKVAAYD    KMAFF
Sbjct: 348 NFYIVGRDPAGMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFF 407

Query: 425 DPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASSQ 478
           DPSR++DFLFISGTKMR  A+  ENPPDGFMCP GWKVLV+YY+SL AE  + +
Sbjct: 408 DPSRSQDFLFISGTKMRGLAKKKENPPDGFMCPSGWKVLVDYYDSLSAETGNGR 461

BLAST of PI0003193 vs. ExPASy Swiss-Prot
Match: O95340 (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 OS=Homo sapiens OX=9606 GN=PAPSS2 PE=1 SV=2)

HSP 1 Score: 499.6 bits (1285), Expect = 4.0e-140
Identity = 234/391 (59.85%), Postives = 300/391 (76.73%), Query Frame = 0

Query: 80  DLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGWASPLKGYMREDEYLQSLHFNC 139
           +L VPE++      EAE LP + +T++DL+WV V+SEGWA+PLKG+MRE EYLQ +HF+ 
Sbjct: 226 ELFVPENKLDHVRAEAETLPSLSITKLDLQWVQVLSEGWATPLKGFMREKEYLQVMHFD- 285

Query: 140 IKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGDLVGILRSIEIFKHNKEERI 199
             + D   +NMS+PIVL +  + K ++   S   +L   G  V ILR  E ++H KEER 
Sbjct: 286 -TLLDDGVINMSIPIVLPVSAEDKTRLEGCSKF-VLAHGGRRVAILRDAEFYEHRKEERC 345

Query: 200 ARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDGLDHYRLSPKQLRQEFDRRQ 259
           +R WGTT    P++ +++  +G+WL+GGDL+V+  I++NDGLD YRL+P +L+Q+     
Sbjct: 346 SRVWGTTCTKHPHI-KMVMESGDWLVGGDLQVLEKIRWNDGLDQYRLTPLELKQKCKEMN 405

Query: 260 ADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPLGGFTKADDVPLDVRMEQ 319
           ADAVFAFQLRNPVHNGHALLM DTR+RLL+ GYK+P+LLLHPLGG+TK DDVPLD RM+Q
Sbjct: 406 ADAVFAFQLRNPVHNGHALLMQDTRRRLLERGYKHPVLLLHPLGGWTKDDDVPLDWRMKQ 465

Query: 320 HSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPT 379
           H+ VLE+GVLDP++TIV+IFPSPM YAGPTEVQWH ++R+ AGANFYIVGRDPAGM HP 
Sbjct: 466 HAAVLEEGVLDPKSTIVAIFPSPMLYAGPTEVQWHCRSRMIAGANFYIVGRDPAGMPHPE 525

Query: 380 EKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKMAFFDPSRAKDFLFISGTKM 439
            K+DLY+P HG KVLSMAPGL  + I+PF+VAAY+   K M F+DP+R  +F FISGT+M
Sbjct: 526 TKKDLYEPTHGGKVLSMAPGLTSVEIIPFRVAAYNKAKKAMDFYDPARHNEFDFISGTRM 585

Query: 440 RTYARTGENPPDGFMCPGGWKVLVNYYESLQ 471
           R  AR GENPPDGFM P  WKVL +YY SL+
Sbjct: 586 RKLAREGENPPDGFMAPKAWKVLTDYYRSLE 612

BLAST of PI0003193 vs. ExPASy TrEMBL
Match: A0A0A0LE71 (Sulfate adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G872050 PE=4 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 1.9e-278
Identity = 473/483 (97.93%), Postives = 478/483 (98.96%), Query Frame = 0

Query: 1   MSLFTKLNIAISPQFTINNLQTTIKTTSIPIYNSKIQSKHIYVSHPLVLFKPTMQGHSPR 60
           MSLFTKL+IAISPQFTINNLQTT KTTSIPIYNSKIQSKHIYVSHPLVLFK TMQ HSPR
Sbjct: 1   MSLFTKLHIAISPQFTINNLQTTKKTTSIPIYNSKIQSKHIYVSHPLVLFKSTMQSHSPR 60

Query: 61  SLRSSIKSSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGWAS 120
           SLRSSIK SLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGWAS
Sbjct: 61  SLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGWAS 120

Query: 121 PLKGYMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGD 180
           PL+G+MREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGD
Sbjct: 121 PLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGD 180

Query: 181 LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDG 240
           LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDG
Sbjct: 181 LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDG 240

Query: 241 LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH 300
           LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH
Sbjct: 241 LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH 300

Query: 301 PLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARIN 360
           PLGGFTKADDVPLDVRMEQHSKVLEDG+LDPETTIVSIFPSPMHYAGPTEVQWHAKARIN
Sbjct: 301 PLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQWHAKARIN 360

Query: 361 AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM 420
           AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM
Sbjct: 361 AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM 420

Query: 421 AFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASSQQAA 480
           AFFDP+RAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEAS QQAA
Sbjct: 421 AFFDPTRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASPQQAA 480

Query: 481 LSV 484
           LSV
Sbjct: 481 LSV 483

BLAST of PI0003193 vs. ExPASy TrEMBL
Match: A0A1S3CRF3 (Sulfate adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103503887 PE=4 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 1.6e-277
Identity = 471/483 (97.52%), Postives = 477/483 (98.76%), Query Frame = 0

Query: 1   MSLFTKLNIAISPQFTINNLQTTIKTTSIPIYNSKIQSKHIYVSHPLVLFKPTMQGHSPR 60
           MSLFTKL+IAISPQFTINNLQTT +TTSIPIYNSKIQSKHIYVSHPLVLFK TMQGHSPR
Sbjct: 1   MSLFTKLHIAISPQFTINNLQTTKRTTSIPIYNSKIQSKHIYVSHPLVLFKSTMQGHSPR 60

Query: 61  SLRSSIKSSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGWAS 120
           SLRSSIKSSLIDPDGGV+VDLVVPESERASR+LEAEPLPKVKLTQIDLEWVHVISEGWAS
Sbjct: 61  SLRSSIKSSLIDPDGGVIVDLVVPESERASRILEAEPLPKVKLTQIDLEWVHVISEGWAS 120

Query: 121 PLKGYMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGD 180
           PL+G+MREDEYLQSLHFNCIKIKDGS VNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGD
Sbjct: 121 PLRGFMREDEYLQSLHFNCIKIKDGSLVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGD 180

Query: 181 LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDG 240
           LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVD VITNAGNWLLGGDLEVINPIKYNDG
Sbjct: 181 LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDVVITNAGNWLLGGDLEVINPIKYNDG 240

Query: 241 LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH 300
           LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH
Sbjct: 241 LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH 300

Query: 301 PLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARIN 360
           PLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARIN
Sbjct: 301 PLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARIN 360

Query: 361 AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM 420
           AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM
Sbjct: 361 AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM 420

Query: 421 AFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASSQQAA 480
           AFFDPSRAKDFLFISGTKMR YARTGENPPDGFMCPGGWKVLVNYYESLQAEEAS QQAA
Sbjct: 421 AFFDPSRAKDFLFISGTKMRAYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASPQQAA 480

Query: 481 LSV 484
           LSV
Sbjct: 481 LSV 483

BLAST of PI0003193 vs. ExPASy TrEMBL
Match: A0A6J1FMY2 (Sulfate adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446642 PE=4 SV=1)

HSP 1 Score: 898.7 bits (2321), Expect = 1.1e-257
Identity = 440/485 (90.72%), Postives = 462/485 (95.26%), Query Frame = 0

Query: 1   MSLFTKLNIAISPQFTINNLQTTIKTTSIPI--YNSKIQSKHIYVSHPLVLFKPTMQGHS 60
           MSL  KL++A SPQ ++N  QTTIK T +PI  Y +KI+SK IYVS  L  FKP MQG+S
Sbjct: 1   MSLVAKLHLATSPQISLNTRQTTIKRTPVPIAAYYTKIKSKPIYVSESLG-FKPAMQGYS 60

Query: 61  PRSLRSSIKSSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGW 120
           PRSLRSSIK SLI+PDGG VV+L+VPESERASRVLEAE LPKVKLT+IDLEWVHVISEGW
Sbjct: 61  PRSLRSSIKCSLIEPDGGAVVELIVPESERASRVLEAESLPKVKLTRIDLEWVHVISEGW 120

Query: 121 ASPLKGYMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPN 180
           ASPLKG+MRE+EYLQSLHFNC+KIKDGS VNMSLPIVLAIGDDVK+QIGSS NVGLLGPN
Sbjct: 121 ASPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIGDDVKEQIGSSPNVGLLGPN 180

Query: 181 GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYN 240
           GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDE+ITNAGNWL+GGDLEVINPIKYN
Sbjct: 181 GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEIITNAGNWLIGGDLEVINPIKYN 240

Query: 241 DGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL 300
           DGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL
Sbjct: 241 DGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL 300

Query: 301 LHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKAR 360
           LHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKAR
Sbjct: 301 LHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKAR 360

Query: 361 INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAK 420
           INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAK
Sbjct: 361 INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAK 420

Query: 421 KMAFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASSQQ 480
           +MAFFDPSRAKDFLFISGTKMRT+ARTGENPPDGFMCPGGWKVLVNYY+SLQAE+ASSQQ
Sbjct: 421 EMAFFDPSRAKDFLFISGTKMRTFARTGENPPDGFMCPGGWKVLVNYYKSLQAEDASSQQ 480

Query: 481 AALSV 484
            ALSV
Sbjct: 481 PALSV 484

BLAST of PI0003193 vs. ExPASy TrEMBL
Match: A0A6J1IXV9 (Sulfate adenylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480215 PE=4 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 7.1e-257
Identity = 438/485 (90.31%), Postives = 463/485 (95.46%), Query Frame = 0

Query: 1   MSLFTKLNIAISPQFTINNLQTTIKTTSIPI--YNSKIQSKHIYVSHPLVLFKPTMQGHS 60
           MSL  KL++A SPQ ++N LQTTIK T +PI  Y +KI+SK IYVS  L  F+P MQG+S
Sbjct: 1   MSLVAKLHLATSPQISLNTLQTTIKRTPVPIATYYTKIKSKPIYVSESLG-FRPAMQGYS 60

Query: 61  PRSLRSSIKSSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGW 120
           PRSLRSSIK SLI+PDGG VV+L+VPESERASRVLEAE LPKVKLT+IDLEWVHVISEGW
Sbjct: 61  PRSLRSSIKCSLIEPDGGAVVELIVPESERASRVLEAESLPKVKLTRIDLEWVHVISEGW 120

Query: 121 ASPLKGYMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPN 180
           ASPLKG+MRE+EYLQSLHFNC+KIKDGS VNMSLPIVLAIGDDVK+QIGSS NVGLLGPN
Sbjct: 121 ASPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIGDDVKEQIGSSPNVGLLGPN 180

Query: 181 GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYN 240
           GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWL+GGDLEVINPIKYN
Sbjct: 181 GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYN 240

Query: 241 DGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL 300
           DGLDHYRLSPKQLRQEFDRR+ADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL
Sbjct: 241 DGLDHYRLSPKQLRQEFDRREADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL 300

Query: 301 LHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKAR 360
           LHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKAR
Sbjct: 301 LHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKAR 360

Query: 361 INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAK 420
           INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDT+AK
Sbjct: 361 INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTMAK 420

Query: 421 KMAFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASSQQ 480
           +MAFF+PSRAKDFLFISGTKMRT+ARTGENPPDGFMCPGGWKVLVNYY+SLQAE+ASSQQ
Sbjct: 421 EMAFFNPSRAKDFLFISGTKMRTFARTGENPPDGFMCPGGWKVLVNYYKSLQAEDASSQQ 480

Query: 481 AALSV 484
            ALSV
Sbjct: 481 PALSV 484

BLAST of PI0003193 vs. ExPASy TrEMBL
Match: A0A6J1BZ55 (Sulfate adenylyltransferase OS=Momordica charantia OX=3673 GN=LOC111006556 PE=4 SV=1)

HSP 1 Score: 876.7 bits (2264), Expect = 4.4e-251
Identity = 426/483 (88.20%), Postives = 455/483 (94.20%), Query Frame = 0

Query: 1   MSLFTKLNIAISPQFTINNLQTTIKTTSIPIYNSKIQSKHIYVSHPLVLFKPTMQGHSPR 60
           MSL TKL+IA SPQF++ NLQ   KTT+I  +++KI+SK IY+S PLV FK  MQ  SPR
Sbjct: 1   MSLLTKLHIATSPQFSL-NLQPATKTTAITNHSAKIKSKTIYISRPLV-FKLPMQASSPR 60

Query: 61  SLRSSIKSSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGWAS 120
           SLRSS+KSSLIDPDGG VVDLVVP+ ERA+RVLEA+PLPKVKL++IDLEWVHVISEGWAS
Sbjct: 61  SLRSSVKSSLIDPDGGAVVDLVVPDGERAARVLEADPLPKVKLSKIDLEWVHVISEGWAS 120

Query: 121 PLKGYMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGD 180
           PL+G+MRE+EYLQSLHFNC+KIKDGS VNMSLPIVLAI DDVKDQIGSS NVGLLGP+GD
Sbjct: 121 PLRGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIADDVKDQIGSSPNVGLLGPSGD 180

Query: 181 LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDG 240
           LVGILRS+EIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWL+GGDLEVINPIKYNDG
Sbjct: 181 LVGILRSVEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYNDG 240

Query: 241 LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH 300
           LDH+RLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH
Sbjct: 241 LDHFRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH 300

Query: 301 PLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARIN 360
           PLGGFTKADDVPLDVRMEQHSK LEDGVLDPETTIV+IFPSPMHYAGPTEVQWHAKARIN
Sbjct: 301 PLGGFTKADDVPLDVRMEQHSKALEDGVLDPETTIVAIFPSPMHYAGPTEVQWHAKARIN 360

Query: 361 AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM 420
           AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPF+VAAYDTVAKKM
Sbjct: 361 AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFRVAAYDTVAKKM 420

Query: 421 AFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASSQQAA 480
           AFF+P RAKDFLFISGTKMR +ARTGENPPDGFMCP GWKVLVNYY+SLQ E+A  QQ A
Sbjct: 421 AFFEPGRAKDFLFISGTKMRNFARTGENPPDGFMCPSGWKVLVNYYQSLQVEDAPPQQPA 480

Query: 481 LSV 484
           LSV
Sbjct: 481 LSV 481

BLAST of PI0003193 vs. NCBI nr
Match: XP_004136390.1 (ATP sulfurylase 2 [Cucumis sativus] >KGN60023.1 hypothetical protein Csa_001197 [Cucumis sativus])

HSP 1 Score: 967.6 bits (2500), Expect = 4.0e-278
Identity = 473/483 (97.93%), Postives = 478/483 (98.96%), Query Frame = 0

Query: 1   MSLFTKLNIAISPQFTINNLQTTIKTTSIPIYNSKIQSKHIYVSHPLVLFKPTMQGHSPR 60
           MSLFTKL+IAISPQFTINNLQTT KTTSIPIYNSKIQSKHIYVSHPLVLFK TMQ HSPR
Sbjct: 1   MSLFTKLHIAISPQFTINNLQTTKKTTSIPIYNSKIQSKHIYVSHPLVLFKSTMQSHSPR 60

Query: 61  SLRSSIKSSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGWAS 120
           SLRSSIK SLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGWAS
Sbjct: 61  SLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGWAS 120

Query: 121 PLKGYMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGD 180
           PL+G+MREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGD
Sbjct: 121 PLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGD 180

Query: 181 LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDG 240
           LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDG
Sbjct: 181 LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDG 240

Query: 241 LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH 300
           LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH
Sbjct: 241 LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH 300

Query: 301 PLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARIN 360
           PLGGFTKADDVPLDVRMEQHSKVLEDG+LDPETTIVSIFPSPMHYAGPTEVQWHAKARIN
Sbjct: 301 PLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQWHAKARIN 360

Query: 361 AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM 420
           AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM
Sbjct: 361 AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM 420

Query: 421 AFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASSQQAA 480
           AFFDP+RAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEAS QQAA
Sbjct: 421 AFFDPTRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASPQQAA 480

Query: 481 LSV 484
           LSV
Sbjct: 481 LSV 483

BLAST of PI0003193 vs. NCBI nr
Match: XP_008466495.1 (PREDICTED: ATP sulfurylase 2 [Cucumis melo])

HSP 1 Score: 964.5 bits (2492), Expect = 3.3e-277
Identity = 471/483 (97.52%), Postives = 477/483 (98.76%), Query Frame = 0

Query: 1   MSLFTKLNIAISPQFTINNLQTTIKTTSIPIYNSKIQSKHIYVSHPLVLFKPTMQGHSPR 60
           MSLFTKL+IAISPQFTINNLQTT +TTSIPIYNSKIQSKHIYVSHPLVLFK TMQGHSPR
Sbjct: 1   MSLFTKLHIAISPQFTINNLQTTKRTTSIPIYNSKIQSKHIYVSHPLVLFKSTMQGHSPR 60

Query: 61  SLRSSIKSSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGWAS 120
           SLRSSIKSSLIDPDGGV+VDLVVPESERASR+LEAEPLPKVKLTQIDLEWVHVISEGWAS
Sbjct: 61  SLRSSIKSSLIDPDGGVIVDLVVPESERASRILEAEPLPKVKLTQIDLEWVHVISEGWAS 120

Query: 121 PLKGYMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGD 180
           PL+G+MREDEYLQSLHFNCIKIKDGS VNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGD
Sbjct: 121 PLRGFMREDEYLQSLHFNCIKIKDGSLVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGD 180

Query: 181 LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDG 240
           LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVD VITNAGNWLLGGDLEVINPIKYNDG
Sbjct: 181 LVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDVVITNAGNWLLGGDLEVINPIKYNDG 240

Query: 241 LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH 300
           LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH
Sbjct: 241 LDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLH 300

Query: 301 PLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARIN 360
           PLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARIN
Sbjct: 301 PLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARIN 360

Query: 361 AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM 420
           AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM
Sbjct: 361 AGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKM 420

Query: 421 AFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASSQQAA 480
           AFFDPSRAKDFLFISGTKMR YARTGENPPDGFMCPGGWKVLVNYYESLQAEEAS QQAA
Sbjct: 421 AFFDPSRAKDFLFISGTKMRAYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASPQQAA 480

Query: 481 LSV 484
           LSV
Sbjct: 481 LSV 483

BLAST of PI0003193 vs. NCBI nr
Match: XP_038899984.1 (ATP sulfurylase 2 [Benincasa hispida])

HSP 1 Score: 928.7 bits (2399), Expect = 2.0e-266
Identity = 456/484 (94.21%), Postives = 469/484 (96.90%), Query Frame = 0

Query: 1   MSLFTKLNIAISPQFTI-NNLQTTIKTTSIPIYNSKIQSKHIYVSHPLVLFKPTMQGHSP 60
           MSL TKL+IAISP+FTI NNLQT IKTTSIP YNSKIQSK IYVSHPL LF+P MQGHSP
Sbjct: 1   MSLVTKLHIAISPKFTINNNLQTIIKTTSIPTYNSKIQSKPIYVSHPL-LFEPKMQGHSP 60

Query: 61  RSLRSSIKSSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGWA 120
           RSLRSSIKSSLIDPDGG +VDLVVPE+ERASRVLEAEPLPKVKLT+IDLEWVHVISEGWA
Sbjct: 61  RSLRSSIKSSLIDPDGGALVDLVVPENERASRVLEAEPLPKVKLTRIDLEWVHVISEGWA 120

Query: 121 SPLKGYMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNG 180
           SPL+G+MRE+EYLQSLHFNC+KIKDGS VNMSLPIVLAI DDVKDQIGSSSNVGLLGP G
Sbjct: 121 SPLRGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIADDVKDQIGSSSNVGLLGPTG 180

Query: 181 DLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYND 240
           DLVGILRSIEIFKHNKEER+ARTWGTT PGLPYVDEVITNAGNWL+GGDLEVINPIKYND
Sbjct: 181 DLVGILRSIEIFKHNKEERMARTWGTTVPGLPYVDEVITNAGNWLIGGDLEVINPIKYND 240

Query: 241 GLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL 300
           GLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL
Sbjct: 241 GLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLL 300

Query: 301 HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI 360
           HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI
Sbjct: 301 HPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARI 360

Query: 361 NAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKK 420
           NAGANFYIVGRDPAGMGHP EKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKK
Sbjct: 361 NAGANFYIVGRDPAGMGHPKEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKK 420

Query: 421 MAFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASSQQA 480
           MAFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAE+AS QQ 
Sbjct: 421 MAFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEDASPQQP 480

Query: 481 ALSV 484
           ALSV
Sbjct: 481 ALSV 483

BLAST of PI0003193 vs. NCBI nr
Match: XP_023525367.1 (ATP sulfurylase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 899.8 bits (2324), Expect = 1.0e-257
Identity = 440/485 (90.72%), Postives = 463/485 (95.46%), Query Frame = 0

Query: 1   MSLFTKLNIAISPQFTINNLQTTIKTTSIPI--YNSKIQSKHIYVSHPLVLFKPTMQGHS 60
           MSL  KL++A SPQ ++N LQTTIK T +P+  Y +KI+SK IYVS  L  FKP MQG+S
Sbjct: 1   MSLVAKLHLATSPQISLNTLQTTIKRTPVPVATYYTKIKSKPIYVSESLG-FKPAMQGYS 60

Query: 61  PRSLRSSIKSSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGW 120
           PRSLRSSIK SLI+PDGG VV+L+VPESERASRVLEAE LPKVKLT+IDLEWVHVISEGW
Sbjct: 61  PRSLRSSIKCSLIEPDGGAVVELIVPESERASRVLEAESLPKVKLTRIDLEWVHVISEGW 120

Query: 121 ASPLKGYMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPN 180
           ASPLKG+MRE+EYLQSLHFNC+KIKDGS VNMSLPIVLAIGDDVK+QIGSS NVGLLGPN
Sbjct: 121 ASPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIGDDVKEQIGSSPNVGLLGPN 180

Query: 181 GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYN 240
           GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWL+GGDLEVINPIKYN
Sbjct: 181 GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYN 240

Query: 241 DGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL 300
           DGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL
Sbjct: 241 DGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL 300

Query: 301 LHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKAR 360
           LHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKAR
Sbjct: 301 LHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKAR 360

Query: 361 INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAK 420
           INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAK
Sbjct: 361 INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAK 420

Query: 421 KMAFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASSQQ 480
           +MAFFDPSRAKDFLFISGTKMRT+ARTGENPPDGFMCPGGWKVLVNYY+SLQAE+A+SQQ
Sbjct: 421 EMAFFDPSRAKDFLFISGTKMRTFARTGENPPDGFMCPGGWKVLVNYYKSLQAEDATSQQ 480

Query: 481 AALSV 484
            ALSV
Sbjct: 481 PALSV 484

BLAST of PI0003193 vs. NCBI nr
Match: KAG6608587.1 (ATP sulfurylase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 898.7 bits (2321), Expect = 2.3e-257
Identity = 440/485 (90.72%), Postives = 462/485 (95.26%), Query Frame = 0

Query: 1   MSLFTKLNIAISPQFTINNLQTTIKTTSIPI--YNSKIQSKHIYVSHPLVLFKPTMQGHS 60
           MSL  KL++A SPQ ++N  QTTIK T +PI  Y +KI+SK IYVS  L  FKP MQG+S
Sbjct: 1   MSLVAKLHLATSPQISLNTRQTTIKRTPVPIATYYTKIKSKPIYVSESLG-FKPAMQGYS 60

Query: 61  PRSLRSSIKSSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGW 120
           PRSLRSSIK SLI+PDGG VV+L+VPESERASRVLEAE LPKVKLT+IDLEWVHVISEGW
Sbjct: 61  PRSLRSSIKCSLIEPDGGAVVELIVPESERASRVLEAESLPKVKLTRIDLEWVHVISEGW 120

Query: 121 ASPLKGYMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPN 180
           ASPLKG+MRE+EYLQSLHFNC+KIKDGS VNMSLPIVLAIGDDVK+QIGSS NVGLLGPN
Sbjct: 121 ASPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIGDDVKEQIGSSPNVGLLGPN 180

Query: 181 GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYN 240
           GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWL+GGDLEVINPIKYN
Sbjct: 181 GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVINPIKYN 240

Query: 241 DGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL 300
           DGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL
Sbjct: 241 DGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL 300

Query: 301 LHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKAR 360
           LHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKAR
Sbjct: 301 LHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKAR 360

Query: 361 INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAK 420
           INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDT+AK
Sbjct: 361 INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTIAK 420

Query: 421 KMAFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASSQQ 480
           +MAFFDPSRAKDFLFISGTKMRT+ARTGENPPDGFMCPGGWKVLVNYY+SLQAE+ASSQQ
Sbjct: 421 EMAFFDPSRAKDFLFISGTKMRTFARTGENPPDGFMCPGGWKVLVNYYKSLQAEDASSQQ 480

Query: 481 AALSV 484
            ALSV
Sbjct: 481 PALSV 484

BLAST of PI0003193 vs. TAIR 10
Match: AT1G19920.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 756.1 bits (1951), Expect = 1.7e-218
Identity = 358/430 (83.26%), Postives = 393/430 (91.40%), Query Frame = 0

Query: 53  TMQGHSPRSLRSSIKSSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVH 112
           TMQ  S    + ++KSSLIDPDGG +V+L+VPE+E   +  E+E +PKVKL QIDLEWVH
Sbjct: 51  TMQSVS----KMTVKSSLIDPDGGELVELIVPETEIGVKKAESETMPKVKLNQIDLEWVH 110

Query: 113 VISEGWASPLKGYMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNV 172
           VISEGWASPLKG+MREDEYLQSLHFN +++K+G+FVNMSLPIVLAI DD K+QIGSS NV
Sbjct: 111 VISEGWASPLKGFMREDEYLQSLHFNSLRLKNGTFVNMSLPIVLAIDDDTKEQIGSSENV 170

Query: 173 GLLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVI 232
            L+ P GD++G LRS+EI+KHNKEERIARTWGTT+PGLPYV+E IT +GNWL+GGDLEV 
Sbjct: 171 ALVCPQGDIIGSLRSVEIYKHNKEERIARTWGTTSPGLPYVEEYITPSGNWLIGGDLEVF 230

Query: 233 NPIKYNDGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGY 292
            PIKYNDGLDHYRLSPKQLR+EFD RQADAVFAFQLRNPVHNGHALLMNDTRKRLL+MGY
Sbjct: 231 EPIKYNDGLDHYRLSPKQLREEFDNRQADAVFAFQLRNPVHNGHALLMNDTRKRLLEMGY 290

Query: 293 KNPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQ 352
           KNP+LLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDP+TTIVSIFPSPMHYAGPTEVQ
Sbjct: 291 KNPVLLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPKTTIVSIFPSPMHYAGPTEVQ 350

Query: 353 WHAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAA 412
           WHAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGK+VLSMAPGLEKLNILPF+VAA
Sbjct: 351 WHAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKRVLSMAPGLEKLNILPFRVAA 410

Query: 413 YDTVAKKMAFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAE 472
           YDT+ KKMAFFDPSRAK+FLFISGTKMRTYARTGENPPDGFMCP GW VLV YYESLQ  
Sbjct: 411 YDTIEKKMAFFDPSRAKEFLFISGTKMRTYARTGENPPDGFMCPSGWNVLVKYYESLQES 470

Query: 473 EASSQQAALS 483
           EA  QQA +S
Sbjct: 471 EA-KQQAVVS 475

BLAST of PI0003193 vs. TAIR 10
Match: AT3G22890.1 (ATP sulfurylase 1 )

HSP 1 Score: 674.1 bits (1738), Expect = 8.4e-194
Identity = 322/443 (72.69%), Postives = 368/443 (83.07%), Query Frame = 0

Query: 36  IQSKHIYVSHPLVLFKPTMQ------GHSPRSLR---SSIKSSLIDPDGGVVVDLVVPES 95
           + SK  ++S PL    P             +SLR    SI++ LI PDGG +V+L+V E 
Sbjct: 7   VLSKTPFLSQPLTKSSPNSDLPFAAVSFPSKSLRRRVGSIRAGLIAPDGGKLVELIVEEP 66

Query: 96  ERASRVLEAEPLPKVKLTQIDLEWVHVISEGWASPLKGYMREDEYLQSLHFNCIKIKDGS 155
           +R  +  EA  LP+V+LT IDL+W+HV+SEGWASPL G+MRE E+LQ+LHFN +++ DGS
Sbjct: 67  KRREKKHEAADLPRVELTAIDLQWMHVLSEGWASPLGGFMRESEFLQTLHFNSLRLDDGS 126

Query: 156 FVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGDLVGILRSIEIFKHNKEERIARTWGTT 215
            VNMS+PIVLAI D+ K +IG S+ V L   +G+ V IL  IEI+KH KEERIARTWGTT
Sbjct: 127 VVNMSVPIVLAIDDEQKARIGESTRVALFNSDGNPVAILSDIEIYKHPKEERIARTWGTT 186

Query: 216 APGLPYVDEVITNAGNWLLGGDLEVINPIKYNDGLDHYRLSPKQLRQEFDRRQADAVFAF 275
           APGLPYVDE ITNAGNWL+GGDLEV+ P+KYNDGLD +RLSP +LR+E ++R ADAVFAF
Sbjct: 187 APGLPYVDEAITNAGNWLIGGDLEVLEPVKYNDGLDRFRLSPAELRKELEKRNADAVFAF 246

Query: 276 QLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPLGGFTKADDVPLDVRMEQHSKVLED 335
           QLRNPVHNGHALLM DTR+RLL+MGYKNPILLLHPLGGFTKADDVPLD RM+QH KVLED
Sbjct: 247 QLRNPVHNGHALLMTDTRRRLLEMGYKNPILLLHPLGGFTKADDVPLDWRMKQHEKVLED 306

Query: 336 GVLDPETTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDLYD 395
           GVLDPETT+VSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHP EKRDLYD
Sbjct: 307 GVLDPETTVVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPVEKRDLYD 366

Query: 396 PDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKMAFFDPSRAKDFLFISGTKMRTYARTG 455
            DHGKKVLSMAPGLE+LNILPF+VAAYD    KMAFFDPSR +DFLFISGTKMRT A+  
Sbjct: 367 ADHGKKVLSMAPGLERLNILPFRVAAYDKTQGKMAFFDPSRPQDFLFISGTKMRTLAKNN 426

Query: 456 ENPPDGFMCPGGWKVLVNYYESL 470
           ENPPDGFMCPGGWKVLV+YYESL
Sbjct: 427 ENPPDGFMCPGGWKVLVDYYESL 449

BLAST of PI0003193 vs. TAIR 10
Match: AT4G14680.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 663.3 bits (1710), Expect = 1.5e-190
Identity = 312/411 (75.91%), Postives = 356/411 (86.62%), Query Frame = 0

Query: 60  RSLRS-SIKSSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGW 119
           RSLR+ S+++ LI+PDGG +VDLVVPE  R  +  EA  LP+V+LT IDL+W+HV+SEGW
Sbjct: 41  RSLRTISVRAGLIEPDGGKLVDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEGW 100

Query: 120 ASPLKGYMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPN 179
           ASPL+G+MRE E+LQ+LHFN + + DGS VNMS+PIVLAI D  K  IG S  V L+  +
Sbjct: 101 ASPLRGFMRESEFLQTLHFNLLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSD 160

Query: 180 GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYN 239
            + + IL  IEI+KH KEERIARTWGTTAPGLPYV+E ITNAG+WL+GGDLEV+ P+KYN
Sbjct: 161 DNPIAILNDIEIYKHPKEERIARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYN 220

Query: 240 DGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL 299
           DGLD +RLSP +LR+E ++R ADAVFAFQLRNPVHNGHALLM DTR+RLL+MGYKNPILL
Sbjct: 221 DGLDRFRLSPFELRKELEKRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILL 280

Query: 300 LHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKAR 359
           LHPLGGFTKADDVPL  RM+QH KVLEDGVLDPETT+VSIFPSPM YAGPTEVQWHAKAR
Sbjct: 281 LHPLGGFTKADDVPLSWRMKQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKAR 340

Query: 360 INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAK 419
           INAGANFYIVGRDPAGMGHP EKRDLYD DHGKKVLSMAPGLE+LNILPF+VAAYD    
Sbjct: 341 INAGANFYIVGRDPAGMGHPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQG 400

Query: 420 KMAFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESL 470
           KMAFFDPSRA+DFLFISGTKMR  A+  ENPPDGFMCPGGWKVLV+YY+SL
Sbjct: 401 KMAFFDPSRAQDFLFISGTKMRALAKNRENPPDGFMCPGGWKVLVDYYDSL 451

BLAST of PI0003193 vs. TAIR 10
Match: AT5G43780.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 655.6 bits (1690), Expect = 3.1e-188
Identity = 311/414 (75.12%), Postives = 355/414 (85.75%), Query Frame = 0

Query: 65  SIKSSLIDPDGGVVVDLVVPESERASRVLEAEPLP-KVKLTQIDLEWVHVISEGWASPLK 124
           S+KS LI+PDGG +++LVV ES R     EAE +P ++KL ++DLEWVHV+SEGWASPLK
Sbjct: 48  SVKSGLIEPDGGKLMNLVVEESRRRVMKHEAETVPARIKLNRVDLEWVHVLSEGWASPLK 107

Query: 125 GYMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGDLVG 184
           G+MR+ E+LQ+LHFN  +++DGS VNMS+PIVLAI DD K +IG S+ V L+   G+ + 
Sbjct: 108 GFMRQSEFLQTLHFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIA 167

Query: 185 ILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDGLDH 244
           IL  IEI+KH KEERIARTWGTTA GLPY +E IT AGNWL+GGDL+V+ PIKYNDGLD 
Sbjct: 168 ILNDIEIYKHPKEERIARTWGTTARGLPYAEEAITKAGNWLIGGDLQVLEPIKYNDGLDR 227

Query: 245 YRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPLG 304
           +RLSP QLR+EF RR ADAVFAFQLRNPVHNGHALLM DTR+RLL+MGYKNP+LLL+PLG
Sbjct: 228 FRLSPSQLREEFIRRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLNPLG 287

Query: 305 GFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQWHAKARINAGA 364
           GFTKADDVPL  RM QH KVLEDGVLDPETT+VSIFPSPM YAGPTEVQWHAKARINAGA
Sbjct: 288 GFTKADDVPLSWRMRQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGA 347

Query: 365 NFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYDTVAKKMAFF 424
           NFYIVGRDPAGMGHPTEKRDLYD DHGKKVLSMAPGLE+LNILPFKVAAYD    KMAFF
Sbjct: 348 NFYIVGRDPAGMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFF 407

Query: 425 DPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASSQ 478
           DPSR++DFLFISGTKMR  A+  ENPPDGFMCP GWKVLV+YY+SL AE  + +
Sbjct: 408 DPSRSQDFLFISGTKMRGLAKKKENPPDGFMCPSGWKVLVDYYDSLSAETGNGR 461

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q438702.4e-21783.26ATP sulfurylase 2 OS=Arabidopsis thaliana OX=3702 GN=APS2 PE=1 SV=1[more]
Q9LIK91.2e-19272.69ATP sulfurylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS1 PE=1 SV... [more]
O233242.1e-18975.91ATP-sulfurylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS3 PE=1 SV... [more]
Q9S7D84.4e-18775.12ATP sulfurylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS4 PE=2 SV... [more]
O953404.0e-14059.85Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 OS=Homo sapiens OX... [more]
Match NameE-valueIdentityDescription
A0A0A0LE711.9e-27897.93Sulfate adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G872050 PE=4 SV=1[more]
A0A1S3CRF31.6e-27797.52Sulfate adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103503887 PE=4 SV=1[more]
A0A6J1FMY21.1e-25790.72Sulfate adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446642 PE=4 S... [more]
A0A6J1IXV97.1e-25790.31Sulfate adenylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480215 PE=4 SV=... [more]
A0A6J1BZ554.4e-25188.20Sulfate adenylyltransferase OS=Momordica charantia OX=3673 GN=LOC111006556 PE=4 ... [more]
Match NameE-valueIdentityDescription
XP_004136390.14.0e-27897.93ATP sulfurylase 2 [Cucumis sativus] >KGN60023.1 hypothetical protein Csa_001197 ... [more]
XP_008466495.13.3e-27797.52PREDICTED: ATP sulfurylase 2 [Cucumis melo][more]
XP_038899984.12.0e-26694.21ATP sulfurylase 2 [Benincasa hispida][more]
XP_023525367.11.0e-25790.72ATP sulfurylase 2 [Cucurbita pepo subsp. pepo][more]
KAG6608587.12.3e-25790.72ATP sulfurylase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G19920.11.7e-21883.26Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
AT3G22890.18.4e-19472.69ATP sulfurylase 1 [more]
AT4G14680.11.5e-19075.91Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
AT5G43780.13.1e-18875.12Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.400.10Sulfate adenylyltransferasecoord: 69..237
e-value: 9.8E-56
score: 189.8
NoneNo IPR availablePANTHERPTHR11055BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASEcoord: 52..478
NoneNo IPR availablePANTHERPTHR11055:SF51ENDOGLUCANASEcoord: 52..478
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 243..466
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 238..471
e-value: 5.6E-95
score: 319.0
IPR002650Sulphate adenylyltransferaseTIGRFAMTIGR00339TIGR00339coord: 73..463
e-value: 4.9E-126
score: 418.5
IPR002650Sulphate adenylyltransferaseCDDcd00517ATPScoord: 98..465
e-value: 3.58099E-167
score: 473.658
IPR025980ATP-sulfurylase PUA-like domainPFAMPF14306PUA_2coord: 71..233
e-value: 9.3E-43
score: 145.6
IPR024951Sulphate adenylyltransferase catalytic domainPFAMPF01747ATP-sulfurylasecoord: 243..464
e-value: 1.3E-66
score: 224.1
IPR015947PUA-like superfamilySUPERFAMILY88697PUA domain-likecoord: 73..237

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0003193.1PI0003193.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009970 cellular response to sulfate starvation
biological_process GO:0016310 phosphorylation
biological_process GO:0000103 sulfate assimilation
cellular_component GO:0009507 chloroplast
molecular_function GO:0004020 adenylylsulfate kinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004781 sulfate adenylyltransferase (ATP) activity