PI0003152 (gene) Melon (PI 482460) v1

Overview


Name	PI0003152
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	short-chain dehydrogenase TIC 32, chloroplastic-like
Location	chr06: 30240725 .. 30242422 (-)
RNA-Seq Expression	PI0003152
Synteny	PI0003152

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTGAAATGCTCAAGTACCTCGTCGGCTCCGCCGGAGCAAGCGGCTACGGCTCCAAATCCACCGCCGAAGAAGTCACTCAATCTTCTACTCATCTCAACTCTATCACCGCCATTGTCACAGGTTCTTTTATCTCAACTCCGAATTTTGATTTTCATTATGATTCCATTAATACACTCACTAACAAATTTTGGCGCAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTAGCCAAGCGCGGCGCAAGGATCATCATTCCAGCTCGCAATCTAAAAGCAGCCGAAGAAGCAAAAGCGAGAATCGTATCGGAATTGGAATGTTTCGATCAATCACGAATCACGGTGATGGTTCTCGATCTTAGCTCTCTGAATTCCGTCATGAACTTCGTCTCCGAGTTCGAGTCCTTAAATTTGCCTCTTAATCTTCTCATGTAATTCATCTAAAAACATTGCTAATTTCACTCCGTTATTGAATCGAACGAACAAACGGCATTTAATTGATCGAAGAAACTCAAACTCAGTTTCGCGTTTCTAATTTCCAGAAACAACGCAGGCAGGTTCTGTTACGAACATGCGATTTCGGAAGACGGAATTGAAATGACCTTCGCTACTAATTATCTAGGCAAGAACATTACAAACAGAACAAATTTATTCCGATCATCTCCTTTAATCATGAGGCCATTCACCATTACTGATCGAATTGATCAATTCGATAATGCAGGTCACTTTCTCTTGACTAAACTTCTGTTGAACAAGATGATAGAAACGGCCAAAGCGACGGGAATCCAAGGACGAATAGTGAATGTGACATCCAACATCCACAGCTGGTTTTCCGGCGACATATTCGAATATCTCAGCCAGATAAGCCGGAACAACAATAACAACAATAGGTACGAGCAATTCTGCTTTCCTTATGAATTAATGGCGCGCTGATCATAGTGATTCTGATAATCATTCAAAGCAGAGAGTACGACGAGACACGCGCTTACGCGTTCTCCAAGCTCGCAAATCTCTTGCACACCGTACAACTAGCTCATAGACTCCAGGTTTTCTTCCATAGATCATAATCATTGCGATTTTCACGAAACGATTTCACATGCGGGTTGAATACTGACAAAATCGAAAACAGGAAATGAAGGCAAATGTTACGGTAAACTGTGTTCATCCGGGAGTTGTGAGAACAAATTTGAACAGAGATCGTGAAGGCTTCATAAAAGGTATATTACTGATCATCAAATTAAACCTTCTTCTACATGGGAATTAAATCAATCAATCTAAATCGCATATAAGAATGATTAATTTTGAATGAGAATACAGATTTGATTTTCTTTATGGCGTCCAAATCAAAGTTGTTGAAGACGATTCCTCAAAGTGCAGCAACAAGTTGTTACGTAGCCACGAATCGGAAAGTGGGAAATGTGAATGGAAAATATTTTGCGGATTGCAATGAACAAGGAAGCTGTGAATCCAATACCAAATCAGCGCACTTGGCTGAACGTCTATGGTCTGCCTCTGAAATCATCGTCTCCAATTTAACTGGTAAGCCTCAACCCTAATCTCAACAATTCAATCGATCAATTTGGATTGAGTTGGTTATGAACTTTTATAATATGGTTTGATTATACGAGTTCAAATAAATTAATAATAACCATATATTATCCTTTTTTTTTCATACTTTTGAAAAATAT

mRNA sequence

ATGCTTGAAATGCTCAAGTACCTCGTCGGCTCCGCCGGAGCAAGCGGCTACGGCTCCAAATCCACCGCCGAAGAAGTCACTCAATCTTCTACTCATCTCAACTCTATCACCGCCATTGTCACAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTAGCCAAGCGCGGCGCAAGGATCATCATTCCAGCTCGCAATCTAAAAGCAGCCGAAGAAGCAAAAGCGAGAATCGTATCGGAATTGGAATGTTTCGATCAATCACGAATCACGGTGATGGTTCTCGATCTTAGCTCTCTGAATTCCGTCATGAACTTCGTCTCCGAGTTCGAGTCCTTAAATTTGCCTCTTAATCTTCTCATAAACAACGCAGGCAGGTTCTGTTACGAACATGCGATTTCGGAAGACGGAATTGAAATGACCTTCGCTACTAATTATCTAGGTCACTTTCTCTTGACTAAACTTCTGTTGAACAAGATGATAGAAACGGCCAAAGCGACGGGAATCCAAGGACGAATAGTGAATGTGACATCCAACATCCACAGCTGGTTTTCCGGCGACATATTCGAATATCTCAGCCAGATAAGCCGGAACAACAATAACAACAATAGAGAGTACGACGAGACACGCGCTTACGCGTTCTCCAAGCTCGCAAATCTCTTGCACACCGTACAACTAGCTCATAGACTCCAGGAAATGAAGGCAAATGTTACGGTAAACTGTGTTCATCCGGGAGTTGTGAGAACAAATTTGAACAGAGATCGTGAAGGCTTCATAAAAGATTTGATTTTCTTTATGGCGTCCAAATCAAAGTTGTTGAAGACGATTCCTCAAAGTGCAGCAACAAGTTGTTACGTAGCCACGAATCGGAAAGTGGGAAATGTGAATGGAAAATATTTTGCGGATTGCAATGAACAAGGAAGCTGTGAATCCAATACCAAATCAGCGCACTTGGCTGAACGTCTATGGTCTGCCTCTGAAATCATCGTCTCCAATTTAACTGGTAAGCCTCAACCCTAATCTCAACAATTCAATCGATCAATTTGGATTGAGTTGGTTATGAACTTTTATAATATGGTTTGATTATACGAGTTCAAATAAATTAATAATAACCATATATTATCCTTTTTTTTTCATACTTTTGAAAAATAT

Coding sequence (CDS)

ATGCTTGAAATGCTCAAGTACCTCGTCGGCTCCGCCGGAGCAAGCGGCTACGGCTCCAAATCCACCGCCGAAGAAGTCACTCAATCTTCTACTCATCTCAACTCTATCACCGCCATTGTCACAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTAGCCAAGCGCGGCGCAAGGATCATCATTCCAGCTCGCAATCTAAAAGCAGCCGAAGAAGCAAAAGCGAGAATCGTATCGGAATTGGAATGTTTCGATCAATCACGAATCACGGTGATGGTTCTCGATCTTAGCTCTCTGAATTCCGTCATGAACTTCGTCTCCGAGTTCGAGTCCTTAAATTTGCCTCTTAATCTTCTCATAAACAACGCAGGCAGGTTCTGTTACGAACATGCGATTTCGGAAGACGGAATTGAAATGACCTTCGCTACTAATTATCTAGGTCACTTTCTCTTGACTAAACTTCTGTTGAACAAGATGATAGAAACGGCCAAAGCGACGGGAATCCAAGGACGAATAGTGAATGTGACATCCAACATCCACAGCTGGTTTTCCGGCGACATATTCGAATATCTCAGCCAGATAAGCCGGAACAACAATAACAACAATAGAGAGTACGACGAGACACGCGCTTACGCGTTCTCCAAGCTCGCAAATCTCTTGCACACCGTACAACTAGCTCATAGACTCCAGGAAATGAAGGCAAATGTTACGGTAAACTGTGTTCATCCGGGAGTTGTGAGAACAAATTTGAACAGAGATCGTGAAGGCTTCATAAAAGATTTGATTTTCTTTATGGCGTCCAAATCAAAGTTGTTGAAGACGATTCCTCAAAGTGCAGCAACAAGTTGTTACGTAGCCACGAATCGGAAAGTGGGAAATGTGAATGGAAAATATTTTGCGGATTGCAATGAACAAGGAAGCTGTGAATCCAATACCAAATCAGCGCACTTGGCTGAACGTCTATGGTCTGCCTCTGAAATCATCGTCTCCAATTTAACTGGTAAGCCTCAACCCTAA

Protein sequence

MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGARIIIPARNLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNLLINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSNIHSWFSGDIFEYLSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVTVNCVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGKYFADCNEQGSCESNTKSAHLAERLWSASEIIVSNLTGKPQP

Homology

[Close]

BLAST of PI0003152 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 7.6e-69
Identity = 161/339 (47.49%), Postives = 217/339 (64.01%), Query Frame = 0


Query: 2   LEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGAR 61
           +E+   + G AG SGYGS STAE+VT  S     +TAI+TG TSGIG E ARVL  RGA 
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTH-SIDAKHLTAIITGGTSGIGLEAARVLGMRGAH 60

Query: 62  IIIPARNLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNLL 121
           +II +RN KAA ++K  I   L+ +  +RI  + LDLSS+ SV +F+ +F +LN+PLN+L
Sbjct: 61  VIIASRNTKAANDSKEMI---LQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNIL 120

Query: 122 INNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSNI 181
           INNAG       +SEDGIE  FATN++GHFLLT LLL+KM  +A+ +GI+GRIVN++S  
Sbjct: 121 INNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIA 180

Query: 182 H--SWFSGDIFEYLSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVT 241
           H  ++  G +F+Y+        N+   Y E +AY  SKLANLLH+  L+ +LQE   N+T
Sbjct: 181 HTYTYTEGIMFDYI--------NDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNIT 240

Query: 242 VNCVHPGVVRTNLNRDR---EGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNV 301
           +N VHPG++ TNL R        +K + FF      L K IPQ AAT+CYVA +  + +V
Sbjct: 241 INSVHPGLITTNLFRHSGLGMAVLKAMSFF------LWKNIPQGAATTCYVALHPDLKDV 300

Query: 302 NGKYFADCNEQGSCESN-TKSAHLAERLWSASEIIVSNL 335
            GKYFADCN   +  SN      LA++LW  S  +V +L
Sbjct: 301 TGKYFADCNV--TTPSNFAIDTTLADKLWDFSIKLVESL 319

[Close]

BLAST of PI0003152 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.3e-68
Identity = 161/339 (47.49%), Postives = 215/339 (63.42%), Query Frame = 0


Query: 2   LEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGAR 61
           +E+   + G AG SGYGS STAE+VT  S     +TAI+TG TSGIG E ARVL  RGA 
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTH-SIDAKHLTAIITGGTSGIGLEAARVLGMRGAH 60

Query: 62  IIIPARNLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNLL 121
           +II ARN KAA ++K  I   L+ +  +RI  + LDLSS+ SV +F+ +F +LN+PLN+L
Sbjct: 61  VIIAARNTKAANDSKEMI---LQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNIL 120

Query: 122 INNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSNI 181
           INNAG       +SEDGIE  FATN++GHFLLT LLL+KM  +A+ +GI+GRIVN++S  
Sbjct: 121 INNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIA 180

Query: 182 H--SWFSGDIFEYLSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVT 241
           H  ++  G +F+Y+        N+   Y E +AY  SKLANLLH+  L+ +LQE   N+T
Sbjct: 181 HTYTYTEGIMFDYI--------NDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNIT 240

Query: 242 VNCVHPGVVRTNLNRDR---EGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNV 301
           +N VHPG++ TNL R        +K + FF      L K IPQ AAT+CYVA +  +  V
Sbjct: 241 INSVHPGLITTNLFRHSGLGMAVLKAMSFF------LWKNIPQGAATTCYVALHPDLKGV 300

Query: 302 NGKYFADCNEQGSCESN-TKSAHLAERLWSASEIIVSNL 335
            GKYF DCN   +  SN      LA++LW  S  +V +L
Sbjct: 301 TGKYFTDCNV--TTPSNFAIDTTLADKLWDFSIKLVDSL 319

[Close]

BLAST of PI0003152 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 5.8e-61
Identity = 142/331 (42.90%), Postives = 200/331 (60.42%), Query Frame = 0


Query: 7   YLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGARIIIPA 66
           +  GS GASG+ S+STAEEVT        +TAIVTGA+SGIG ETARVL+ RG  +++  
Sbjct: 2   WFFGSKGASGFSSRSTAEEVTH-GVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAV 61

Query: 67  RNLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNLLINNAG 126
           RN  +  + K  IV ++     +++ VM LDLSS+ SV  F SE++S  LPLNLLINNAG
Sbjct: 62  RNTDSGAKVKEDIVKQV---PGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAG 121

Query: 127 RFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSNIHSWFS 186
                  +S+D IE+ FATN+LGHFLLTKLLL+ M  T++ +  +GRIVN++S  H    
Sbjct: 122 IMACPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHR--- 181

Query: 187 GDIFEYLSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVTVNCVHPG 246
              F Y   +  +  N+   Y   RAY  SKL N+LH  +L  +L+E   N+T N +HPG
Sbjct: 182 ---FSYPEGVRFDKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPG 241

Query: 247 VVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGKYFADCNE 306
            + TNL R    ++   +  +A    +LK++PQ AAT+CYVA N +V  V+G+YF D N 
Sbjct: 242 AIMTNLGRYFNPYLAVAVGAVA--KYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNI 301

Query: 307 QGSCESNTKSAHLAERLWSASEIIVSNLTGK 338
                   K   LA+++W  S  +  + +G+
Sbjct: 302 AKPLPL-VKDTELAKKVWDFSTKLTDSQSGE 319

[Close]

BLAST of PI0003152 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 6.6e-57
Identity = 139/315 (44.13%), Postives = 188/315 (59.68%), Query Frame = 0


Query: 13  GASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGARIIIPARNLKAA 72
           G SG+   STAE+VT        +TAIVTGA+SGIG ET RVLA RGA +I+  RN+ AA
Sbjct: 9   GVSGFSGSSTAEQVTH-GIDATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMVAA 68

Query: 73  EEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNLLINNAGRFCYEH 132
           ++ K  I+ ++     +++  + LDLSSL+SV  F SEF S   PLN+LINNAG      
Sbjct: 69  KDVKDTILKDI---PSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPF 128

Query: 133 AISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSNIHSWFSGDIFEY 192
            +S+D IE+ FATN++GHFLLT LLL+ M +T + +  +GRIVNV S  H       F Y
Sbjct: 129 KLSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHR------FAY 188

Query: 193 LSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVTVNCVHPGVVRTNL 252
              I  +  N+   Y+  RAY  SKLAN+LH  QL   L+E   N+T N +HPG + TNL
Sbjct: 189 PEGIRFDKINDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNL 248

Query: 253 NRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGKYFADCNEQGSCES 312
            R     +  LI  +     +LK + Q AAT+CYVA + +V  V+G+YF+D N       
Sbjct: 249 FRHNSA-VNGLINVIG--KLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVY-KTTP 308

Query: 313 NTKSAHLAERLWSAS 328
           + K   LA++LW  S
Sbjct: 309 HGKDVDLAKKLWDFS 309

[Close]

BLAST of PI0003152 vs. ExPASy Swiss-Prot
Match: Q9HBH5 (Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 6.2e-39
Identity = 118/308 (38.31%), Postives = 169/308 (54.87%), Query Frame = 0


Query: 37  TAIVTGATSGIGEETARVLAKRGARIIIPARNLKAAEEAKARIVSEL----ECFDQ---- 96
           T ++TGA SG+G  TA  L + GAR+I+  R+   AEEA  ++  EL    EC  +    
Sbjct: 45  TVLITGANSGLGRATAAELLRLGARVIMGCRDRARAEEAAGQLRRELRQAAECGPEPGVS 104

Query: 97  --SRITVMVLDLSSLNSVMNFVSEFESLNLPLNLLINNAGRFCYEHAISEDGIEMTFATN 156
               + V  LDL+SL SV  F  E       L++LINNAG F   +  +EDG EM F  N
Sbjct: 105 GVGELIVRELDLASLRSVRAFCQEMLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQFGVN 164

Query: 157 YLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSNIHSWFSGDIFEYLSQISRNNNNNNRE 216
           +LGHFLLT LLL  +  +A +     RIV V+S ++ +  GD       I+ ++ N+ + 
Sbjct: 165 HLGHFLLTNLLLGLLKSSAPS-----RIVVVSSKLYKY--GD-------INFDDLNSEQS 224

Query: 217 YDETRAYAFSKLANLLHTVQLAHRLQEMKANVTVNCVHPGVVRTNLNRDREGFIKDLIFF 276
           Y+++  Y+ SKLAN+L T +LA RL+    NVTVN +HPG+VRTNL R     +     F
Sbjct: 225 YNKSFCYSRSKLANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHIPLLVKPLF 284

Query: 277 MASKSKLLKTIPQSAATSCYVATNRKVGNVNGKYFADCNEQGSCESNTKSAHLAERLWSA 335
                   KT  + A TS Y+A++ +V  V+G+YF DC E+           +A +LW  
Sbjct: 285 NLVSWAFFKTPVEGAQTSIYLASSPEVEGVSGRYFGDCKEE-ELLPKAMDESVARKLWDI 335

[Close]

BLAST of PI0003152 vs. ExPASy TrEMBL
Match: A0A1S3BLF4 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103491110 PE=4 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 1.8e-177
Identity = 325/340 (95.59%), Postives = 332/340 (97.65%), Query Frame = 0


Query: 1   MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60
           MLEMLKYLVGSAGASGYGS STAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIIIPARNLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNL 120
           RI+IPARNLKAAEEAKARIVSELECFD SRITVMVLDLSSLNSVMNFVSEFE+LNLPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNKMIETAK+TGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVTV 240
           IHSWFSGDIFEYLSQISRN NNNNREYD TRAY+FSKLANLLHTVQLAHRLQEMKANVTV
Sbjct: 181 IHSWFSGDIFEYLSQISRNYNNNNREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVTV 240

Query: 241 NCVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGKY 300
           NCVHPGVVRTNLNRDREGF+KDLIFFMASKSKLLKTIPQSAATSCYVAT+RKV NVNGKY
Sbjct: 241 NCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGKY 300

Query: 301 FADCNEQGSCESNTKSAHLAERLWSASEIIVSNLTGKPQP 341
           F DCNEQGSCESNTKSAHLAERLWSASEII+SNLTG PQP
Sbjct: 301 FEDCNEQGSCESNTKSAHLAERLWSASEIIISNLTGNPQP 340

[Close]

BLAST of PI0003152 vs. ExPASy TrEMBL
Match: A0A5A7UCR1 (Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold344G00880 PE=4 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 1.9e-176
Identity = 325/341 (95.31%), Postives = 333/341 (97.65%), Query Frame = 0


Query: 1   MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60
           MLEMLKYLVGSAGASGYGS STAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIIIPARNLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNL 120
           RI+IPARNLKAAEEAKARIVSELECFD SRITVMVLDLSSLNSVMNFVSEFE+LNLPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNKMIETAK+TGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLSQISRN-NNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVT 240
           IHSWFSGDIFEYLSQISRN NNNN+REYD TRAY+FSKLANLLHTVQLAHRLQEMKANVT
Sbjct: 181 IHSWFSGDIFEYLSQISRNYNNNNSREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVT 240

Query: 241 VNCVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGK 300
           VNCVHPGVVRTNLNRDREGF+KDLIFFMASKSKLLKTIPQSAATSCYVAT+RKV NVNGK
Sbjct: 241 VNCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGK 300

Query: 301 YFADCNEQGSCESNTKSAHLAERLWSASEIIVSNLTGKPQP 341
           YF DCNEQGSCESNTKSAHLAERLWSASEII+SNLTG PQP
Sbjct: 301 YFEDCNEQGSCESNTKSAHLAERLWSASEIIISNLTGNPQP 341

[Close]

BLAST of PI0003152 vs. ExPASy TrEMBL
Match: A0A6J1EB90 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430995 PE=4 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 2.4e-158
Identity = 293/340 (86.18%), Postives = 313/340 (92.06%), Query Frame = 0


Query: 1   MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60
           MLEM KYLVGSAGASGYGSKSTAEEV++S   +NSITAIVTGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIIIPARNLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNL 120
           RIIIPARNLKAAEEAKARIVSE +CFD SRITVM LDLSS+NSV+NFVS FESLNLPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLN+MIETAK+TG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVTV 240
           IHSWFSGDIFEYL QIS+    N+R YD TRAYAFSKLAN+LHTVQLAH+LQEMKANVTV
Sbjct: 181 IHSWFSGDIFEYLGQISQ---KNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTV 240

Query: 241 NCVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGKY 300
           NCVHPGVV+TNLNR+REGF+ DLIF+MASKS+LLKTIP+ AATSCYVATNR V NVNG Y
Sbjct: 241 NCVHPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNY 300

Query: 301 FADCNEQGSCESNTKSAHLAERLWSASEIIVSNLTGKPQP 341
           FADCN+QGSCE  TKSAHLAE LWSASE IVS LTGKPQP
Sbjct: 301 FADCNDQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335

[Close]

BLAST of PI0003152 vs. ExPASy TrEMBL
Match: A0A6J1E6B3 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430995 PE=4 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 3.1e-158
Identity = 293/340 (86.18%), Postives = 312/340 (91.76%), Query Frame = 0


Query: 1   MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60
           MLEM KYLVGSAGASGYGSKSTAEEV++S   +NSITAIVTGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIIIPARNLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNL 120
           RIIIPARNLKAAEEAKARIVSE +CFD SRITVM LDLSS+NSV+NFVS FESLNLPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLN+MIETAK+TG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVTV 240
           IHSWFSGDIFEYL QIS+     NR YD TRAYAFSKLAN+LHTVQLAH+LQEMKANVTV
Sbjct: 181 IHSWFSGDIFEYLGQISQ----KNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTV 240

Query: 241 NCVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGKY 300
           NCVHPGVV+TNLNR+REGF+ DLIF+MASKS+LLKTIP+ AATSCYVATNR V NVNG Y
Sbjct: 241 NCVHPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNY 300

Query: 301 FADCNEQGSCESNTKSAHLAERLWSASEIIVSNLTGKPQP 341
           FADCN+QGSCE  TKSAHLAE LWSASE IVS LTGKPQP
Sbjct: 301 FADCNDQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 334

[Close]

BLAST of PI0003152 vs. ExPASy TrEMBL
Match: A0A6J1HN85 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465182 PE=4 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.2e-157
Identity = 292/340 (85.88%), Postives = 313/340 (92.06%), Query Frame = 0


Query: 1   MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60
           MLEM KYLVGSAGASGYGSKSTAEEV++S   +NSITAIVTGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60

Query: 61  RIIIPARNLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNL 120
           RIIIPARNLKAAEEAKARIVSE  CFD+SRITVM LDLSS+ SV+NFVS FESLNLPLNL
Sbjct: 61  RIIIPARNLKAAEEAKARIVSESNCFDRSRITVMALDLSSVKSVVNFVSNFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSN 180
           LINNAGRFCYEHA+SEDGIEMTFATNYLGHFLLTKLLLN+MIETAK+TG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHALSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVTV 240
           IHSWFSGDIFEYL QIS+    N+R YD TRAYAFSKLAN+LHTVQLAH+LQEMKANVTV
Sbjct: 181 IHSWFSGDIFEYLGQISQ---KNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTV 240

Query: 241 NCVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGKY 300
           NCVHPGVV+TNLNR+REGF+ DLIF+MASKS+LLK IP+ AATSCYVATNR V NVNGKY
Sbjct: 241 NCVHPGVVKTNLNREREGFVTDLIFYMASKSRLLKIIPEGAATSCYVATNRNVENVNGKY 300

Query: 301 FADCNEQGSCESNTKSAHLAERLWSASEIIVSNLTGKPQP 341
           FADCN+QGSCE  TKSAHLAE LWSASE IVS LTGKPQP
Sbjct: 301 FADCNDQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335

[Close]

BLAST of PI0003152 vs. NCBI nr
Match: XP_008449162.1 (PREDICTED: short-chain dehydrogenase TIC 32, chloroplastic-like [Cucumis melo])

HSP 1 Score: 631.7 bits (1628), Expect = 3.6e-177
Identity = 325/340 (95.59%), Postives = 332/340 (97.65%), Query Frame = 0


Query: 1   MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60
           MLEMLKYLVGSAGASGYGS STAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIIIPARNLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNL 120
           RI+IPARNLKAAEEAKARIVSELECFD SRITVMVLDLSSLNSVMNFVSEFE+LNLPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNKMIETAK+TGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVTV 240
           IHSWFSGDIFEYLSQISRN NNNNREYD TRAY+FSKLANLLHTVQLAHRLQEMKANVTV
Sbjct: 181 IHSWFSGDIFEYLSQISRNYNNNNREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVTV 240

Query: 241 NCVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGKY 300
           NCVHPGVVRTNLNRDREGF+KDLIFFMASKSKLLKTIPQSAATSCYVAT+RKV NVNGKY
Sbjct: 241 NCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGKY 300

Query: 301 FADCNEQGSCESNTKSAHLAERLWSASEIIVSNLTGKPQP 341
           F DCNEQGSCESNTKSAHLAERLWSASEII+SNLTG PQP
Sbjct: 301 FEDCNEQGSCESNTKSAHLAERLWSASEIIISNLTGNPQP 340

[Close]

BLAST of PI0003152 vs. NCBI nr
Match: KAA0052978.1 (short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa])

HSP 1 Score: 628.2 bits (1619), Expect = 4.0e-176
Identity = 325/341 (95.31%), Postives = 333/341 (97.65%), Query Frame = 0


Query: 1   MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60
           MLEMLKYLVGSAGASGYGS STAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIIIPARNLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNL 120
           RI+IPARNLKAAEEAKARIVSELECFD SRITVMVLDLSSLNSVMNFVSEFE+LNLPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNKMIETAK+TGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLSQISRN-NNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVT 240
           IHSWFSGDIFEYLSQISRN NNNN+REYD TRAY+FSKLANLLHTVQLAHRLQEMKANVT
Sbjct: 181 IHSWFSGDIFEYLSQISRNYNNNNSREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVT 240

Query: 241 VNCVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGK 300
           VNCVHPGVVRTNLNRDREGF+KDLIFFMASKSKLLKTIPQSAATSCYVAT+RKV NVNGK
Sbjct: 241 VNCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGK 300

Query: 301 YFADCNEQGSCESNTKSAHLAERLWSASEIIVSNLTGKPQP 341
           YF DCNEQGSCESNTKSAHLAERLWSASEII+SNLTG PQP
Sbjct: 301 YFEDCNEQGSCESNTKSAHLAERLWSASEIIISNLTGNPQP 341

[Close]

BLAST of PI0003152 vs. NCBI nr
Match: XP_011650304.1 (short-chain dehydrogenase TIC 32 A, chloroplastic isoform X1 [Cucumis sativus] >KAE8650011.1 hypothetical protein Csa_011335 [Cucumis sativus])

HSP 1 Score: 628.2 bits (1619), Expect = 4.0e-176
Identity = 327/340 (96.18%), Postives = 332/340 (97.65%), Query Frame = 0


Query: 1   MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60
           MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAK GA
Sbjct: 1   MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKGGA 60

Query: 61  RIIIPARNLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNL 120
           RI+IPARNLKAAEEAKARIVSELECFD SRITVMVLDLSSLNSVMNFVSEFESLNLPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDHSRITVMVLDLSSLNSVMNFVSEFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSN 180
           LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETA++TGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAESTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVTV 240
           IHSWFSGD+FEYLSQISR NNN  REYD TRAYAFSKLANLLHTVQLAHRLQEMKANVTV
Sbjct: 181 IHSWFSGDVFEYLSQISR-NNNKYREYDATRAYAFSKLANLLHTVQLAHRLQEMKANVTV 240

Query: 241 NCVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGKY 300
           NCVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVAT+RKV NVNGKY
Sbjct: 241 NCVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGKY 300

Query: 301 FADCNEQGSCESNTKSAHLAERLWSASEIIVSNLTGKPQP 341
           FADCNEQGSCESNTKSAHLAERLWS SEIIVSNLTGKPQP
Sbjct: 301 FADCNEQGSCESNTKSAHLAERLWSTSEIIVSNLTGKPQP 339

[Close]

BLAST of PI0003152 vs. NCBI nr
Match: XP_038876602.1 (short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 594.3 bits (1531), Expect = 6.4e-166
Identity = 309/337 (91.69%), Postives = 319/337 (94.66%), Query Frame = 0


Query: 4   MLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGARII 63
           ML+YLVGSAGASGYGSKSTAEEVT+S THL+SIT IVTGATSGIGEET RVLAKRGARI+
Sbjct: 1   MLRYLVGSAGASGYGSKSTAEEVTRSCTHLSSITVIVTGATSGIGEETTRVLAKRGARIV 60

Query: 64  IPARNLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNLLIN 123
           IPARNLKAAEEAK RIVSELECFD SRITVMVLDLSSLNSVMNFVSEFESLNLPLN+LIN
Sbjct: 61  IPARNLKAAEEAKTRIVSELECFDHSRITVMVLDLSSLNSVMNFVSEFESLNLPLNVLIN 120

Query: 124 NAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSNIHS 183
           NAGRFCYE A SEDGIEMTFATNYLGHFLLTKLLLNKM ETAK+TGIQGRIVNVTSNIHS
Sbjct: 121 NAGRFCYERASSEDGIEMTFATNYLGHFLLTKLLLNKMTETAKSTGIQGRIVNVTSNIHS 180

Query: 184 WFSGDIFEYLSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVTVNCV 243
            FSGDIFEYLSQISR  NNNNREYD TRAYAFSKLAN+LHTVQLAHRLQEMKANVTVNCV
Sbjct: 181 GFSGDIFEYLSQISR--NNNNREYDATRAYAFSKLANVLHTVQLAHRLQEMKANVTVNCV 240

Query: 244 HPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGKYFAD 303
           HPGVVRTNLNRDREGFI DLIF++ASKSKLLKTIPQSAATSCYVATNR V NVNGKYFAD
Sbjct: 241 HPGVVRTNLNRDREGFITDLIFYLASKSKLLKTIPQSAATSCYVATNRNVENVNGKYFAD 300

Query: 304 CNEQGSCESNTKSAHLAERLWSASEIIVSNLTGKPQP 341
           CNEQGSC+SNTKSAHLAERLWSASE IVS L GKPQP
Sbjct: 301 CNEQGSCDSNTKSAHLAERLWSASETIVSTLNGKPQP 335

[Close]

BLAST of PI0003152 vs. NCBI nr
Match: XP_031737895.1 (short-chain dehydrogenase TIC 32 A, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 579.7 bits (1493), Expect = 1.6e-161
Identity = 307/340 (90.29%), Postives = 312/340 (91.76%), Query Frame = 0


Query: 1   MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60
           MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAK GA
Sbjct: 1   MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKGGA 60

Query: 61  RIIIPARNLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNL 120
           RI+IPARNLKAAEEAKARIVSELECFD SRITVMVLDLSSLNSVMNFVSEFESLNLPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDHSRITVMVLDLSSLNSVMNFVSEFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSN 180
           LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETA++TGIQGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAESTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVTV 240
           IHSWFSGD+FEYLSQISR NNN  REYD TRAYAFSKLANLLHTVQLAHRLQEMKANVTV
Sbjct: 181 IHSWFSGDVFEYLSQISR-NNNKYREYDATRAYAFSKLANLLHTVQLAHRLQEMKANVTV 240

Query: 241 NCVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGKY 300
           NCVHPGVVRTNLNRDREGFIK                    ATSCYVAT+RKV NVNGKY
Sbjct: 241 NCVHPGVVRTNLNRDREGFIK--------------------ATSCYVATHRKVENVNGKY 300

Query: 301 FADCNEQGSCESNTKSAHLAERLWSASEIIVSNLTGKPQP 341
           FADCNEQGSCESNTKSAHLAERLWS SEIIVSNLTGKPQP
Sbjct: 301 FADCNEQGSCESNTKSAHLAERLWSTSEIIVSNLTGKPQP 319

[Close]

BLAST of PI0003152 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 424.5 bits (1090), Expect = 8.2e-119
Identity = 228/332 (68.67%), Postives = 273/332 (82.23%), Query Frame = 0


Query: 1   MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60
           M+E  KYL+G+AGASG+GSKSTAEEVT+ +  L SITA++TGATSGIG ETARVLAKRGA
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTE-NCDLRSITAVITGATSGIGAETARVLAKRGA 60

Query: 61  RIIIPARNLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNL 120
           R+I PARN+KAAEEAK RIVSE   F ++ I VM LDLSS+ SV NFV++FESL+LPLNL
Sbjct: 61  RLIFPARNVKAAEEAKERIVSE---FPETEIVVMKLDLSSIASVRNFVADFESLDLPLNL 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSN 180
           LINNAG+  +EHAISEDGIEMTFATNYLGHFLLT LLLNKMI+TA+ TG+QGRIVNVTS 
Sbjct: 121 LINNAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSG 180

Query: 181 IHSWFSGDIFEYLSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVTV 240
           IH WFSGD+ EYL  IS+       ++D TRAYA SKLAN+LHT +L+ RLQ++ ANVTV
Sbjct: 181 IHGWFSGDLIEYLRLISQ----PKCQFDATRAYALSKLANVLHTKELSSRLQKIGANVTV 240

Query: 241 NCVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGKY 300
           NCVHPGVVRT L RDREG + DL+FF+A  SKL+KT+PQ+AAT+CYVATN ++ NV+GKY
Sbjct: 241 NCVHPGVVRTRLTRDREGLLTDLVFFLA--SKLVKTVPQAAATTCYVATNPRLVNVSGKY 300

Query: 301 FADCNEQGSCESNTKSAHLAERLWSASEIIVS 333
           F DCNE       T S+  A +LW+ASEI+V+
Sbjct: 301 FTDCNETTPSGLGTNSSE-ATKLWAASEILVT 321

[Close]

BLAST of PI0003152 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 416.0 bits (1068), Expect = 2.9e-116
Identity = 217/332 (65.36%), Postives = 272/332 (81.93%), Query Frame = 0


Query: 1   MLEMLKYLVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60
           M+E +K+L+GS G SG+GS+STA+ VT +S  L S+TAI+TGATSGIG ETARVLAKRGA
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVTCNS-DLRSLTAIITGATSGIGAETARVLAKRGA 60

Query: 61  RIIIPARNLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNL 120
           R+++PAR++K AEE KARI+SE   F  + I VM LDLSSL SV  FV +FESLNLPLN+
Sbjct: 61  RLVLPARSVKTAEETKARILSE---FPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNI 120

Query: 121 LINNAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSN 180
           LINNAG++ ++HA+SEDG+EMTFATNYLGHFLLTKLLL KMIETA  TG+QGRIVNVTS 
Sbjct: 121 LINNAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSV 180

Query: 181 IHSWFSGDIFEYLSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVTV 240
           +HSWFSGD+ +YL+ ISRNN N    YD TRAYA SKLAN+LHTV+L+  L +M ANVT 
Sbjct: 181 VHSWFSGDMLQYLADISRNNRN----YDATRAYALSKLANVLHTVELSRLLHKMDANVTA 240

Query: 241 NCVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGKY 300
           NCVHPG+V+T L RDREG + DL+FF+   SKLLK++PQ+AAT+CYVAT+ ++ NV GKY
Sbjct: 241 NCVHPGIVKTRLTRDREGVVTDLVFFLT--SKLLKSVPQAAATTCYVATSPRLRNVCGKY 300

Query: 301 FADCNEQGSCESNTKSAHLAERLWSASEIIVS 333
           F+DCNE  S +S + +   A+RLW+AS+++VS
Sbjct: 301 FSDCNEARSSKSGSCNLK-AQRLWTASDLLVS 321

[Close]

BLAST of PI0003152 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 288.9 bits (738), Expect = 5.4e-78
Identity = 163/331 (49.24%), Postives = 227/331 (68.58%), Query Frame = 0


Query: 5   LKYLVGSAGASGYGSKSTAEEVTQSSTH-LNSITAIVTGATSGIGEETARVLAKRGARII 64
           L+YL G AG +G+GS+STAE+VTQ S    + +TAI+TG TSGIG ETARVLAKRG R++
Sbjct: 5   LRYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHLTAIITGGTSGIGAETARVLAKRGVRVV 64

Query: 65  IPARNLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNLLIN 124
           +  R++K AE  K RI+ E     ++ I +  +DLSSL+SV  F S+F S +LPLN+LIN
Sbjct: 65  MAVRDMKKAEMVKERIIRE---NPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNILIN 124

Query: 125 NAGRFCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSNIHS 184
           NAG F      SE+ IE+TFATN+LGH+LLT++L+ KMI+TA+ +GI+GRI+N++S IH+
Sbjct: 125 NAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVIHN 184

Query: 185 WFSGDIFEY---LSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVTV 244
           W   D F +   L  ISR        Y+ TRAYA SKLA +LH   L+ +L++  ANVT+
Sbjct: 185 WVKPDCFSFPKLLHPISR--------YNGTRAYAQSKLATILHAKALSKQLKDRNANVTI 244

Query: 245 NCVHPGVVRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGKY 304
           N VHPG+V+T + R  +G   D +F +A  SKLLK+I Q AAT+CYVA + +   ++GKY
Sbjct: 245 NAVHPGIVKTGIIRAHKGLFTDSLFLIA--SKLLKSISQGAATTCYVALSNETKGLSGKY 304

Query: 305 FADCNEQGSCESNTKSAHLAERLWSASEIIV 332
           FADCNE  +C       ++A +L + S  ++
Sbjct: 305 FADCNET-NCSDLANDEYVALKLCTQSRALI 321

[Close]

BLAST of PI0003152 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.2e-72
Identity = 160/330 (48.48%), Postives = 216/330 (65.45%), Query Frame = 0


Query: 8   LVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGARIIIPAR 67
           + G  G SG+GS STAEEVTQ     N +TAI+TG T GIG ETARVL+KRGA ++I AR
Sbjct: 7   ITGRRGPSGFGSASTAEEVTQGIDATN-LTAIITGGTGGIGMETARVLSKRGAHVVIGAR 66

Query: 68  NLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNLLINNAGR 127
           N+ AAE AK  I+ +      +R+T++ LDLSS+ S+  FV EF +L+LPLNLLINNAG 
Sbjct: 67  NMGAAENAKTEILRQNA---NARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINNAGV 126

Query: 128 FCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSNIHSWFSG 187
               + +SEDGIE+ FATN++GHFLLT LLL+ M  TAK +G++GRI+NV+S  H     
Sbjct: 127 MFCPYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAH----- 186

Query: 188 DIFEYLSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVTVNCVHPGV 247
            I+ Y   I  ++ N+   Y + RAY  SKLAN+LH  +L+ +LQE   N+T N VHPG+
Sbjct: 187 -IYTYQEGIQFDSINDICSYSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHPGL 246

Query: 248 VRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGKYFADCNEQ 307
           + TNL +     ++ L FF      L K IPQ AAT+CYVA +  V  V GKYFADCNE 
Sbjct: 247 ILTNLFQHTALLMRFLKFF---SFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNEV 306

Query: 308 GSCESNTKSAHLAERLWSASEIIVSNLTGK 338
            +     +   LA++LW  S  ++++++ K
Sbjct: 307 -TPSKLARDETLAQKLWDFSVKLINSVSKK 322

[Close]

BLAST of PI0003152 vs. TAIR 10
Match: AT2G37540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 259.2 bits (661), Expect = 4.5e-69
Identity = 156/328 (47.56%), Postives = 210/328 (64.02%), Query Frame = 0


Query: 8   LVGSAGASGYGSKSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGARIIIPAR 67
           + G  G SG+GS STAE+VTQ +   + +TAI+TG TSGIG E ARVLA RGA +II AR
Sbjct: 7   MTGKKGKSGFGSASTAEDVTQ-AIDASHLTAIITGGTSGIGLEAARVLAMRGAHVIIAAR 66

Query: 68  NLKAAEEAKARIVSELECFDQSRITVMVLDLSSLNSVMNFVSEFESLNLPLNLLINNAGR 127
           N KAA E+K  I   L+    +R+  + +D+SS+ SV +FV +F +LN+PLN+LINNAG 
Sbjct: 67  NPKAANESKEMI---LQMNPNARVDYLQIDVSSIKSVRSFVDQFLALNVPLNILINNAGV 126

Query: 128 FCYEHAISEDGIEMTFATNYLGHFLLTKLLLNKMIETAKATGIQGRIVNVTSNIHSWFSG 187
                 ++EDGIE  FATN++GHFLLT LLL+KM  TA+ +G+QGRIVN++S  H+    
Sbjct: 127 MFCPFKLTEDGIESQFATNHIGHFLLTNLLLDKMKSTARESGVQGRIVNLSSIAHT---- 186

Query: 188 DIFEYLSQISRNNNNNNREYDETRAYAFSKLANLLHTVQLAHRLQEMKANVTVNCVHPGV 247
             + Y   I     N+   Y E RAY  SKL+NLLH+  L+ RLQE   N+T+N VHPG+
Sbjct: 187 --YTYSEGIKFQGINDPAGYSERRAYGQSKLSNLLHSNALSRRLQEEGVNITINSVHPGL 246

Query: 248 VRTNLNRDREGFIKDLIFFMASKSKLLKTIPQSAATSCYVATNRKVGNVNGKYFADCNEQ 307
           V TNL R   GF   +  F A      K IPQ AAT+CYVA +  +  V GKYF DCN  
Sbjct: 247 VTTNLFR-YSGF--SMKVFRAMTFLFWKNIPQGAATTCYVALHPDLEGVTGKYFGDCNIV 306

Query: 308 GSCESNTKSAHLAERLWSASEIIVSNLT 336
              +  T ++ LA++LW  S  ++ +++
Sbjct: 307 APSKFATNNS-LADKLWDFSVFLIDSIS 320

The following BLAST results are available for this feature:

BLAST of PI0003152 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A078IS66	7.6e-69	47.49	Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A0A078ISJ6	1.3e-68	47.49	Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A2RVM0	5.8e-61	42.90	Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q6RVV4	6.6e-57	44.13	Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3...	[more]
Q9HBH5	6.2e-39	38.31	Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1	[more]

BLAST of PI0003152 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BLF4	1.8e-177	95.59	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=...	[more]
A0A5A7UCR1	1.9e-176	95.31	Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A6J1EB90	2.4e-158	86.18	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita mos...	[more]
A0A6J1E6B3	3.1e-158	86.18	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita mos...	[more]
A0A6J1HN85	1.2e-157	85.88	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita max...	[more]

BLAST of PI0003152 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008449162.1	3.6e-177	95.59	PREDICTED: short-chain dehydrogenase TIC 32, chloroplastic-like [Cucumis melo]	[more]
KAA0052978.1	4.0e-176	95.31	short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa]	[more]
XP_011650304.1	4.0e-176	96.18	short-chain dehydrogenase TIC 32 A, chloroplastic isoform X1 [Cucumis sativus] >...	[more]
XP_038876602.1	6.4e-166	91.69	short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa his...	[more]
XP_031737895.1	1.6e-161	90.29	short-chain dehydrogenase TIC 32 A, chloroplastic isoform X2 [Cucumis sativus]	[more]

BLAST of PI0003152 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24050.1	8.2e-119	68.67	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G64590.1	2.9e-116	65.36	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.1	5.4e-78	49.24	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G02540.1	1.2e-72	48.48	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G37540.1	4.5e-69	47.56	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 116..127 score: 42.55 coord: 213..232 score: 29.8 coord: 166..182 score: 27.62 coord: 236..253 score: 36.91 coord: 37..54 score: 46.16
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 37..181 e-value: 5.7E-26 score: 91.2
None	No IPR available	GENE3D	3.40.50.720		coord: 23..334 e-value: 3.8E-71 score: 241.5
None	No IPR available	PANTHER	PTHR24320:SF210	MITOCHONDRIAL PYRUVATE CARRIER	coord: 4..334
None	No IPR available	PANTHER	PTHR24320	RETINOL DEHYDROGENASE	coord: 4..334
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 37..306

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0003152.1	PI0003152.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity