PI0003149 (gene) Melon (PI 482460) v1

Overview


Name	PI0003149
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	AdoMet-dependent rRNA methyltransferase SPB1
Location	chr08: 22678534 .. 22682880 (-)
RNA-Seq Expression	PI0003149
Synteny	PI0003149

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCAAAGCATCAAGAGATAAGAGGGTTTAAGCTCTATTCTATTTGACTATTTGTATTCCTGATTCATTATCTTTTACCATGTTTTGTTTACTTGGTCTCATTCGCAGGATATATACTACCGTAAAGCAAAAGAAGAAGGTTGGCGTGCTCGAAGTGCATTTAAACTTCTTCAGATAGATGAGGAATTTAACATATTTGAAGGTTTGTTCTTGTATTGAGCTCCTCTATTAAAATATTGTTACATTTCTGGTAGGATGTCAATCACTGAGATGGTCATGAGAATTAATCTTTCTGCAACATTTTGGATTAACCTTCCTAACAGAGTTGTTTTGTGTGATCTTTATGACTTTGTAATCATCCTGTTTTATCATGTACAAGTATTTCGAAATGCCTCTATGTACATCGTCTACAATGAAATTTCATATGCAGGAGTCAAGAGAGTGGTAGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTATTTCTTTTGAGCGTCTTCTTGTTGCAATTTGTGTATCAAACTAGTGGTCAATGACTCAATGCCTTATGGGGAAAACAAATTTTTGTATAAAATACAAATAAACCTAAGAAGAATTTGTCTTGCGATAGACTTTGTAAGCATCTTTTTTGTTTGGAAAGCATGTCTGTATGTGGGGTGCAATTCTCTCGTCCCTTCATTGACCCTGCATCATGGCACACTGAGTACGTTTTGATAAACGCTAAATATAATGAGGGCTGATAAAAGCAATGTAGTATGTGTTGATGCAACTATTACTTACAAATATAGTTTATTGTGGTTAATTATTTCAAAGTTGTCCTGACATTTTCACGCCTACTAATCATATATAGGTCCTCAGTAGAAAATTGTATCTCCCAGCAAAGCTTTCTCCAGATTTAAAGTAAGTATGGTAGATTATAATATGAAAATGTAGGCCTCGTTAGTAATCATTTTGTGTTTGGTTTTTAGTTTTTGAAAAGTAAACTTGTTTTCTCTCAATTTCTGATAATGATTTACATTTTTCTTAAGTGAAAGAGTTGAGTTCTTAACCAAATTCCAAAAACAAAAACAAGTTTTTCAAGACTATTTTTTAGTTTTCAAATTTGGCTTGGTTTTTAAAAACAACAGTAAAAGCTGAAAAGTAGTCAACAAATTAAGAAATTTAAGAGTGGAAGGGTGTTTATAGGCTTAATTTTTAAAAATTTAACACTAAAACCAGATGGTTACCAAATGGGGTCGTAACTTTTCCGTCCATCCTTCTCTCCATTGTCCCCTTGTTATCTCCCTCTTTCATTGATTCTCTAGCTTTATTTGCAGGGATGGCGATCTTCCTCTTATTGTTGCTATTGATTTACAGCCGATGGCTCCAATTGAAGGTGTTATTGAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTAAGCAGAGTGTTTCATGTACACACTATTTTTAACATTTGTTATGGTAAACGAAGACATATCCAGATCCTGATGCACCAACATGGTCAATTTTGTTTTAAAACTTCCATTTTGTGATAATTTTCCTACGTTTTTCTTCATACGGGATTACTTTTCTCTTTTGAATGAGTACTCATGCTGTGTTGTAAGTCCTTTTTCTTTTATTAAAAAAATTGTCACATACGAAACTGTGATATCTTTTTATTCATGGTTTATCAAATATTTAATTTTACCAGGTCATTAGGCATTTTGATGGGTGCAAGGCTGACCTGGTTGTTTGTGACGGTGCACCTGATGGTAGATATTTGTGTTTTTTGAATTCTCTGTTGGGATTCCACCTGATGATATTTTTGTTACTGATTATGTGCTTGTGTTTGTAGTTACTGGTCTTCATGACATGGACGAGTTTGTTCAGTTCCAGCTCATACTAGCTGTAAGTGCAGTGTTTTTTCTACAGACTGTTTAATTTCTCTAGATGGTGCTGATGAATAGTTTAAGATATTTTTGAAAATGCATGAGATGCATGCACATTGACAGTAATGATGTTGCTCCAGTTCTCGTTTACAGTGCAGGGAGAGGATAAAAAAATAAAACCAACAAGAAGCACTGATGAGTGTCAAATTGTTTGAATCTACAAATAGAAAATATTGTGTAACAAGTGAGCAAGGTAGATGAGATCCTTTAGGTTAATCGTGGTTGCTGTTTATTATGAAGTTATATCGAAATCTTCTAAAGGTTTTTCTATTGTATGTTGCCTGCACTCGTTGACCTTTTTCCTCTATGGATTGAATTTTGTTGCTGTCATCAGTGTCCTCTGCTGCTGCCTCATTTTCTCTATTTCTCTCTCTTCCTTCAGCTAGTTCTCAATGTAGTTGGAGATGTATGAGTTTTACAGAGATGAGCTGGACTCTTTTTCCCTTTCTTCTCAGGGTTTAACAATTGTTACGCACATACTCAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACCAGCCTTTTGTATAGTCAGGTAAATGGTTGTTACAGGACCCAATAGAAATATGTAGCATACTCGTGTGTAAAGAAATTTATGGTGTTCTCACTAAATGCATCTCTAATATGAATCAGGATCTCTCTTTGAACTTTTCTTCTTCCAATCAAGTTGGAAATTTTGCTTTGAGCTCCTTTTCCCTGTAATTATTGCATTGACGTATATGATTTTTTTAGATATGTTTAGCACTAATAAAAATTTTCAAGTATTATCATGGATTACCTAAGCAGCTTAATCAAGGGTGTTTGTTGTTTCATACATGGTTATGAAGCCGCCAATGATTTTTACATTTTTGAGTTTTTCTGTTGCAGCTGAAGTTATTTTCCCTGTAGTGACTTTTGCAAAGCCAAAAAGCAGTCGGAATTCAAGTATAGGTAACCTTTCGGTTTCCACTTTTGTTGATTGGTTGCTATAGAGGTCCATCTTTCGATTATAATCGTTCTTGTTTCCAGAGAATGATATTCTCTTAATTGAAAATGGCCTCTCTCTCTCTTTTATTTTATTTTTTTAATCTCTTTATCGCGTCTGGTTCATAGGATTCTTGTGCATGAATTTTTAGATACCCATTGTACTTGGATTCCTTAAATCAACTTTAGATTGTTATCGATTAATACTAGCTGGGTGGACTGGTGTCCGCTCAACAGCAAATTGTGGAGTTGGTACATTTAGGGGTGGTCTGTAATATAAAAAAGGTGTGAAAGAGGACAAAATAGTCAAGGGTAAAAGTAAAATTTTTGAACTTTCACTGTTGCTTTTGTGAACATCCATGAATCCAAATAGTCAAATGTTTGATTATGGTAAGTCTCCTTTTATCACTTGCTTAAATGCTTTAGTAAGCAGTGATTATTTGGCATTACATTTTAAATTGAGTTGTTTGGTTTTTCTGCCCTTATTTAGTTGGCTTTCTCTTATATGTCACACGGTGGTATATCATACAAGATCATTATTTGTTAACGTTAGTCAAATCAAAGTTCCAAGAAAACATTTAAGCTACGGTATTAACCATAAGAAATTACTAGGATAAAAAATCAAGAGTTCAAGAACATAATTAGAACGTCTGAAAGTTGAATGGACTAAGTGGGTACAAGTATTAAAATTATTGAACTAAATTTGTAATTCATCCCAGTGCTTTCTTTTAGTTGCCTAGGTCAACGCACGGTAACAACTTTTAAACAAAGTTGAGATTATGATTTAACTTTATGTCGAAGCTCATACGTTGACTGGGTGCAAATGCAGAGGCATTTGCAGTTTGTGAGAACTATTCTTCTCCCGAAGGTTTCAATCCAAAAGATCTGCATCGCTTGCTGGAAAAGGTGGGAAGTCCGTCAGGAGGAGATGACTTAGGTAAACTCAATATCCTACCTTGATTTGGAGTTCACATTTCGATAGACGTATCCTAAGATCAACAATGTCCTTATCCTATTTTAAAAGATTAATTTGTTTTACAGCTGGCACTGTGACCGAGCTGTCTACTTGTCTCTAGGCTTTTAAAAATTAGTGTGTCTGCTGATTTTGATGTGGTCAATGCCCTGGTATTTTGGTTACATAAGAGGAAGTAATAGCATTTCTATTTCGCTACATTTTGTCCCTTTCAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCTGAGGGAACTTATCGGAGCTTAGATCCTGTGCAGCCTCCTATTGCCCCTCCATACAAGAGGGCTCTCGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTTTCCTTGGATTCCTGA

mRNA sequence

ATGGGCAAAGCATCAAGAGATAAGAGGGATATATACTACCGTAAAGCAAAAGAAGAAGGTTGGCGTGCTCGAAGTGCATTTAAACTTCTTCAGATAGATGAGGAATTTAACATATTTGAAGGAGTCAAGAGAGTGGTAGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTCCTCAGTAGAAAATTGTATCTCCCAGCAAAGCTTTCTCCAGATTTAAAGGATGGCGATCTTCCTCTTATTGTTGCTATTGATTTACAGCCGATGGCTCCAATTGAAGGTGTTATTGAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTCATTAGGCATTTTGATGGGTGCAAGGCTGACCTGGTTGTTTGTGACGGTGCACCTGATGTTACTGGTCTTCATGACATGGACGAGTTTGTTCAGTTCCAGCTCATACTAGCTGGTTTAACAATTGTTACGCACATACTCAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACCAGCCTTTTGTATAGTCAGGTAAATGCTGAAGTTATTTTCCCTGTAGTGACTTTTGCAAAGCCAAAAAGCAGTCGGAATTCAAGTATAGAGGCATTTGCAGTTTGTGAGAACTATTCTTCTCCCGAAGGTTTCAATCCAAAAGATCTGCATCGCTTGCTGGAAAAGGTGGGAAGTCCGTCAGGAGGAGATGACTTAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCTGAGGGAACTTATCGGAGCTTAGATCCTGTGCAGCCTCCTATTGCCCCTCCATACAAGAGGGCTCTCGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTTTCCTTGGATTCCTGA

Coding sequence (CDS)

ATGGGCAAAGCATCAAGAGATAAGAGGGATATATACTACCGTAAAGCAAAAGAAGAAGGTTGGCGTGCTCGAAGTGCATTTAAACTTCTTCAGATAGATGAGGAATTTAACATATTTGAAGGAGTCAAGAGAGTGGTAGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTCCTCAGTAGAAAATTGTATCTCCCAGCAAAGCTTTCTCCAGATTTAAAGGATGGCGATCTTCCTCTTATTGTTGCTATTGATTTACAGCCGATGGCTCCAATTGAAGGTGTTATTGAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTCATTAGGCATTTTGATGGGTGCAAGGCTGACCTGGTTGTTTGTGACGGTGCACCTGATGTTACTGGTCTTCATGACATGGACGAGTTTGTTCAGTTCCAGCTCATACTAGCTGGTTTAACAATTGTTACGCACATACTCAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACCAGCCTTTTGTATAGTCAGGTAAATGCTGAAGTTATTTTCCCTGTAGTGACTTTTGCAAAGCCAAAAAGCAGTCGGAATTCAAGTATAGAGGCATTTGCAGTTTGTGAGAACTATTCTTCTCCCGAAGGTTTCAATCCAAAAGATCTGCATCGCTTGCTGGAAAAGGTGGGAAGTCCGTCAGGAGGAGATGACTTAGATTGCAGTAGTGGGTGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATACCCACTGCCCAGAGGTGCTGAGGGAACTTATCGGAGCTTAGATCCTGTGCAGCCTCCTATTGCCCCTCCATACAAGAGGGCTCTCGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTTTCCTTGGATTCCTGA

Protein sequence

MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQVNAEVIFPVVTFAKPKSSRNSSIEAFAVCENYSSPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIRELEKLSLDS

Homology

[Close]

BLAST of PI0003149 vs. ExPASy Swiss-Prot
Match: Q9UET6 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapiens OX=9606 GN=FTSJ1 PE=1 SV=2)

HSP 1 Score: 345.9 bits (886), Expect = 4.9e-94
Identity = 172/302 (56.95%), Postives = 214/302 (70.86%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MG+ S+DKRD+YYR AKE GWRARSAFKLLQ+D+EF +F+GV R VDLCAAPGSWSQVLS
Sbjct: 1   MGRTSKDKRDVYYRLAKENGWRARSAFKLLQLDKEFQLFQGVTRAVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           +K          +       +VA+DLQ MAP+ GV+++QGDIT   TA+ +I+HF GC A
Sbjct: 61  QK----------IGGQGSGHVVAVDLQAMAPLPGVVQIQGDITQLSTAKEIIQHFKGCPA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQV 180
           DLVVCDGAPDVTGLHD+DE++Q QL+LA L I TH+L+ GG F+AKIFRG+D +LLYSQ+
Sbjct: 121 DLVVCDGAPDVTGLHDVDEYMQAQLLLAALNIATHVLKPGGCFVAKIFRGRDVTLLYSQL 180

Query: 181 NAEVIFPVVTFAKPKSSRNSSIEAFAVCENYSSPEGFNPKDLHRLLEKVGSPSGGDDLDC 240
             +V F  V  AKP+SSRNSSIEAFAVC+ Y  PEGF P     LL+    P        
Sbjct: 181 --QVFFSSVLCAKPRSSRNSSIEAFAVCQGYDPPEGFIPDLSKPLLDHSYDPDFNQ---- 240

Query: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300
               L+GP ++ +PF+ CGDLS YDSDRSYPL       Y+   P QPPI+PPY+ A  +
Sbjct: 241 ----LDGPTRIIVPFVTCGDLSSYDSDRSYPLDLEGGSEYKYTPPTQPPISPPYQEACTL 282

Query: 301 KK 303
           K+
Sbjct: 301 KR 282

[Close]

BLAST of PI0003149 vs. ExPASy Swiss-Prot
Match: O36015 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4F10.03c PE=3 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.4e-85
Identity = 163/301 (54.15%), Postives = 215/301 (71.43%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MG++S+DKRD YYR AKE+GWRARSAFKLLQ++E+FN+FEG KRVVDLCAAPGSWSQVLS
Sbjct: 1   MGRSSKDKRDAYYRLAKEQGWRARSAFKLLQLNEQFNLFEGAKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           R+L     +   +   + P+IVA+DLQPMAPI+GV  +Q DIT+  T  +++ HF    A
Sbjct: 61  RELL--KNIDTSIAADEKPMIVAVDLQPMAPIDGVCTLQLDITHPNTLSIILSHFGNEPA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQV 180
           DLVV DGAPDVTGLHD+DE++Q Q++LA   +   +L+ GGKF+AKIFRG+D SLLYSQ+
Sbjct: 121 DLVVSDGAPDVTGLHDLDEYIQAQILLAAFNLAVCVLKPGGKFVAKIFRGRDVSLLYSQL 180

Query: 181 NAEVIFPVVTFAKPKSSRNSSIEAFAVCENYSSPEGFNPKDLHRLLEKVGSPSGGDDLDC 240
              ++F  V+ AKP+SSR SSIE+F VCE+++ P  F P DL + L  V  P+   ++  
Sbjct: 181 --RLMFRKVSCAKPRSSRASSIESFVVCEDFNPPSNFQP-DLTKPL-CVIDPTNAHEI-- 240

Query: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300
                        PF+ACGDL GYD+D +YP+    +    +LD +QPP APPYKRA+E+
Sbjct: 241 ------------APFIACGDLDGYDADATYPVEINMKKA--TLDVIQPPTAPPYKRAIEL 279

Query: 301 K 302
           K
Sbjct: 301 K 279

[Close]

BLAST of PI0003149 vs. ExPASy Swiss-Prot
Match: Q22031 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabditis elegans OX=6239 GN=R74.7 PE=3 SV=3)

HSP 1 Score: 312.0 bits (798), Expect = 7.8e-84
Identity = 169/314 (53.82%), Postives = 212/314 (67.52%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGK SRDKRDIYYR AKE  WRARSAFKL+QID+EF I +GV+R VDLCAAPGSWSQVLS
Sbjct: 1   MGKTSRDKRDIYYRLAKENKWRARSAFKLMQIDDEFQILKGVRRAVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           ++LY         ++     IVAIDLQPMAPI GVI++QGDIT+  TA  VI+HF G K+
Sbjct: 61  KRLY---------EEDQEAKIVAIDLQPMAPIPGVIQLQGDITSVDTANQVIKHFSGEKS 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQV 180
           D+V+CDGAPDVTG+H +DEF+Q +LILA   I +H+L+EGG F+AKIFR +++SLLY+Q+
Sbjct: 121 DIVICDGAPDVTGIHSLDEFMQAELILAAFNITSHVLKEGGNFLAKIFRSRNSSLLYAQM 180

Query: 181 NAEVIFPVVTFAKPKSSRNSSIEAFAVCENYSSPEGFNPKDLHRLLEKVGSPSGGDDLDC 240
             +  F  V  AKP+SSR SS EAF +C +YS PEGF P              G   LD 
Sbjct: 181 --KKYFKKVYLAKPRSSRQSSCEAFVLCLDYSPPEGFVP------------TMGKTSLDA 240

Query: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPL------PRG-----AEGTYRSLDPVQPP 300
           +      P+ +   F+ CGDLSG+DS++SYPL      P+G      +  Y   D VQPP
Sbjct: 241 TDASAISPD-IIDGFVTCGDLSGWDSEKSYPLDIDACFPKGEIDEEQKKRYEFKDVVQPP 290

Query: 301 IAPPYKRALEMKKA 304
             P YK AL+ KK+
Sbjct: 301 TDPAYKAALDKKKS 290

[Close]

BLAST of PI0003149 vs. ExPASy Swiss-Prot
Match: Q54VA8 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=fsjA PE=3 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 3.7e-78
Identity = 152/276 (55.07%), Postives = 200/276 (72.46%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGK+S+DKRDIYYRKAKEEGWRARSAFKL+QIDEE+ IFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKSSKDKRDIYYRKAKEEGWRARSAFKLIQIDEEYQIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           R++Y   K  PD+K      IVA+DLQ MAP++GV++++GDIT   T++ +I HFDG  A
Sbjct: 61  RRIYGDGK-DPDVK------IVAVDLQEMAPLKGVVQIKGDITKYETSKQIISHFDGSLA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQV 180
           DL++ DGAPDVTGLHD+D + Q QLIL+ L I TH L+ GG F+AK+F+G D SL+YSQ+
Sbjct: 121 DLIISDGAPDVTGLHDIDFYGQSQLILSALNITTHTLKIGGTFVAKMFKGDDMSLMYSQM 180

Query: 181 NAEVIFPVVTFAKPKSSRNSSIEAFAVCENYSSPEGFNPKDLHRLLE---KVGSPSGGDD 240
             ++ F  V+F KP SSR SS+E F +C NY  P  +NPK +   LE   K+ + +  + 
Sbjct: 181 --KLFFEHVSFVKPSSSRESSLENFILCRNYQPPLNYNPKIIDPALENHNKILNNNNNNS 240

Query: 241 LDCSSGWLEGPN--------KVYIPFLACGDLS-GY 265
            + ++  ++  N         + +PF+ACGDL+ GY
Sbjct: 241 NNNNNCNIDNENDNEFLKTDSLIVPFIACGDLNLGY 267

[Close]

BLAST of PI0003149 vs. ExPASy Swiss-Prot
Match: P38238 (tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TRM7 PE=1 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 6.0e-76
Identity = 156/310 (50.32%), Postives = 212/310 (68.39%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFE--GVKRVVDLCAAPGSWSQV 60
           MGK+S+DKRD+YYRKAKE+G+RARSAFKLLQ++++F+  +   +KRVVDLCAAPGSWSQV
Sbjct: 1   MGKSSKDKRDLYYRKAKEQGYRARSAFKLLQLNDQFHFLDDPNLKRVVDLCAAPGSWSQV 60

Query: 61  LSRKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGC 120
           LSRKL+  +  S    D +   IV++DLQPM+PI  V  +Q DIT+ +T   +++ F   
Sbjct: 61  LSRKLFDESPSS----DKEDRKIVSVDLQPMSPIPHVTTLQADITHPKTLARILKLFGNE 120

Query: 121 KADLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYS 180
           KAD V  DGAPDVTGLHD+DE+VQ QLI++ L +   IL++GG F+AKIFRG+D  +LYS
Sbjct: 121 KADFVCSDGAPDVTGLHDLDEYVQQQLIMSALQLTACILKKGGTFVAKIFRGRDIDMLYS 180

Query: 181 QVNAEVIFPVVTFAKPKSSRNSSIEAFAVCENYSSPEGFNPK-DLHRLLEKVGSPSGGDD 240
           Q+    +F  +  AKP+SSR +S+EAF VC  Y+ P  + PK D++  +++       + 
Sbjct: 181 QLG--YLFDKIVCAKPRSSRGTSLEAFIVCLGYNPPSNWTPKLDVNTSVDEFFQGCFLNK 240

Query: 241 L---DCSSGWLEGPNKVYIPFLACGDLSGYDSDRSY-PLPRGAEGTYRSLDPVQPPIAPP 300
           L   D  S W E    +   F+ACG L  +DSD +Y  LP    GT  SLDPVQ P  PP
Sbjct: 241 LCISDKLSHWNEEERNI-AEFMACGSLQSFDSDATYHDLPSSVAGTSSSLDPVQSPTNPP 300

Query: 301 YKRALEMKKA 304
           YK+ALE+K++
Sbjct: 301 YKKALELKRS 303

[Close]

BLAST of PI0003149 vs. ExPASy TrEMBL
Match: A0A1S3B6C8 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486316 PE=3 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 6.9e-176
Identity = 311/318 (97.80%), Postives = 314/318 (98.74%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQV 180
           DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQ+
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180

Query: 181 NAEVIFPVVTFAKPKSSRNSSIEAFAVCENYSSPEGFNPKDLHRLLEKVGSPSGGDDLDC 240
             ++ FPVVTFAKPKSSRNSSIEAFAVCENYS PEGFNPKDLHRLLEKVGSPSGGDDLDC
Sbjct: 181 --KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDC 240

Query: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300
           SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM
Sbjct: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300

Query: 301 KKASSQGIRELEKLSLDS 319
           KKASSQGIRELEKLSLDS
Sbjct: 301 KKASSQGIRELEKLSLDS 316

[Close]

BLAST of PI0003149 vs. ExPASy TrEMBL
Match: A0A0A0LCV6 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G732420 PE=3 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 1.7e-174
Identity = 309/318 (97.17%), Postives = 313/318 (98.43%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKLYLPAKLSPDLKDG+LPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQV 180
           DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQ+
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180

Query: 181 NAEVIFPVVTFAKPKSSRNSSIEAFAVCENYSSPEGFNPKDLHRLLEKVGSPSGGDDLDC 240
             ++ FPVVTFAKPKSSRNSSIEAFAVCENYS PEGFN KDLHRLLEKVGSPSGGDDLDC
Sbjct: 181 --KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDC 240

Query: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300
           SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM
Sbjct: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300

Query: 301 KKASSQGIRELEKLSLDS 319
           KKASSQGIRELEKLSLDS
Sbjct: 301 KKASSQGIRELEKLSLDS 316

[Close]

BLAST of PI0003149 vs. ExPASy TrEMBL
Match: A0A6J1F1P7 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111441398 PE=3 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.3e-171
Identity = 303/318 (95.28%), Postives = 309/318 (97.17%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKLYLPAKLSPD KDGD PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQV 180
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY Q+
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 181 NAEVIFPVVTFAKPKSSRNSSIEAFAVCENYSSPEGFNPKDLHRLLEKVGSPSGGDDLDC 240
             ++ FPVVTFAKPKSSRNSSIEAFAVCENYS PEGFNPKDLHRLLEKVGSPSGGDDLDC
Sbjct: 181 --KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDC 240

Query: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300
           SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM
Sbjct: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300

Query: 301 KKASSQGIRELEKLSLDS 319
           KKASSQGIRELEKLS DS
Sbjct: 301 KKASSQGIRELEKLSSDS 316

[Close]

BLAST of PI0003149 vs. ExPASy TrEMBL
Match: A0A5A7TQN9 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00950 PE=3 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 5.1e-171
Identity = 302/309 (97.73%), Postives = 305/309 (98.71%), Query Frame = 0


Query: 10  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69
           DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL
Sbjct: 70  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 129

Query: 70  SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129
           SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 130 SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 189

Query: 130 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQVNAEVIFPVV 189
           DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQ+  ++ FPVV
Sbjct: 190 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL--KLFFPVV 249

Query: 190 TFAKPKSSRNSSIEAFAVCENYSSPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPN 249
           TFAKPKSSRNSSIEAFAVCENYS PEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPN
Sbjct: 250 TFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPN 309

Query: 250 KVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIR 309
           KVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIR
Sbjct: 310 KVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIR 369

Query: 310 ELEKLSLDS 319
           ELEKLSLDS
Sbjct: 370 ELEKLSLDS 376

[Close]

BLAST of PI0003149 vs. ExPASy TrEMBL
Match: A0A6J1IYY9 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111481931 PE=3 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 1.6e-169
Identity = 301/318 (94.65%), Postives = 307/318 (96.54%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNI EGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKLYLPAKLSPD KDGD PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQV 180
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY Q+
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 181 NAEVIFPVVTFAKPKSSRNSSIEAFAVCENYSSPEGFNPKDLHRLLEKVGSPSGGDDLDC 240
             ++ FPVVTFAKPKSSRNSSIEAFAVCENYS  EGFNPKDLHRLLEKVGSPSGGDDLDC
Sbjct: 181 --KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDC 240

Query: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300
           SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM
Sbjct: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300

Query: 301 KKASSQGIRELEKLSLDS 319
           KKASSQGIRELEKLS DS
Sbjct: 301 KKASSQGIRELEKLSSDS 316

[Close]

BLAST of PI0003149 vs. NCBI nr
Match: XP_008442455.1 (PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo] >XP_008442456.1 PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo] >XP_008442457.1 PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 626.3 bits (1614), Expect = 1.4e-175
Identity = 311/318 (97.80%), Postives = 314/318 (98.74%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQV 180
           DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQ+
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180

Query: 181 NAEVIFPVVTFAKPKSSRNSSIEAFAVCENYSSPEGFNPKDLHRLLEKVGSPSGGDDLDC 240
             ++ FPVVTFAKPKSSRNSSIEAFAVCENYS PEGFNPKDLHRLLEKVGSPSGGDDLDC
Sbjct: 181 --KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDC 240

Query: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300
           SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM
Sbjct: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300

Query: 301 KKASSQGIRELEKLSLDS 319
           KKASSQGIRELEKLSLDS
Sbjct: 301 KKASSQGIRELEKLSLDS 316

[Close]

BLAST of PI0003149 vs. NCBI nr
Match: XP_004137730.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativus] >XP_011651872.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativus] >XP_031738324.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativus] >KGN58784.1 hypothetical protein Csa_000714 [Cucumis sativus])

HSP 1 Score: 621.7 bits (1602), Expect = 3.5e-174
Identity = 309/318 (97.17%), Postives = 313/318 (98.43%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKLYLPAKLSPDLKDG+LPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQV 180
           DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQ+
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQL 180

Query: 181 NAEVIFPVVTFAKPKSSRNSSIEAFAVCENYSSPEGFNPKDLHRLLEKVGSPSGGDDLDC 240
             ++ FPVVTFAKPKSSRNSSIEAFAVCENYS PEGFN KDLHRLLEKVGSPSGGDDLDC
Sbjct: 181 --KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDC 240

Query: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300
           SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM
Sbjct: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300

Query: 301 KKASSQGIRELEKLSLDS 319
           KKASSQGIRELEKLSLDS
Sbjct: 301 KKASSQGIRELEKLSLDS 316

[Close]

BLAST of PI0003149 vs. NCBI nr
Match: XP_038903231.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa hispida])

HSP 1 Score: 612.1 bits (1577), Expect = 2.8e-171
Identity = 303/318 (95.28%), Postives = 310/318 (97.48%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGV+RVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVERVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKL+LPAKLSPD KDGDLPLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLHLPAKLSPDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQV 180
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY Q+
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 181 NAEVIFPVVTFAKPKSSRNSSIEAFAVCENYSSPEGFNPKDLHRLLEKVGSPSGGDDLDC 240
             ++ FPVVTFAKPKSSRNSSIEAFAVCENYS PEGFNPKDLHRLLEKVGSPSGGDDLDC
Sbjct: 181 --KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDC 240

Query: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300
           SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYR LDPVQPPIAPPYKRALEM
Sbjct: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRCLDPVQPPIAPPYKRALEM 300

Query: 301 KKASSQGIRELEKLSLDS 319
           KKASSQGIRELEKLSLDS
Sbjct: 301 KKASSQGIRELEKLSLDS 316

[Close]

BLAST of PI0003149 vs. NCBI nr
Match: KAG6580486.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 611.3 bits (1575), Expect = 4.7e-171
Identity = 303/318 (95.28%), Postives = 309/318 (97.17%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 21  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 80

Query: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKLYLPAKLSPD KDGD PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKA
Sbjct: 81  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 140

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQV 180
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY Q+
Sbjct: 141 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 200

Query: 181 NAEVIFPVVTFAKPKSSRNSSIEAFAVCENYSSPEGFNPKDLHRLLEKVGSPSGGDDLDC 240
             ++ FPVVTFAKPKSSRNSSIEAFAVCENYS PEGFNPKDLHRLLEKVGSPSGGDDLDC
Sbjct: 201 --KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDC 260

Query: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300
           SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM
Sbjct: 261 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 320

Query: 301 KKASSQGIRELEKLSLDS 319
           KKASSQGIRELEKLS DS
Sbjct: 321 KKASSQGIRELEKLSSDS 336

[Close]

BLAST of PI0003149 vs. NCBI nr
Match: XP_022934133.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita moschata] >XP_022934134.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita moschata] >XP_023528668.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita pepo subsp. pepo] >XP_023528669.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita pepo subsp. pepo] >KAG7017237.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 611.3 bits (1575), Expect = 4.7e-171
Identity = 303/318 (95.28%), Postives = 309/318 (97.17%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           RKLYLPAKLSPD KDGD PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RKLYLPAKLSPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQV 180
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY Q+
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYCQL 180

Query: 181 NAEVIFPVVTFAKPKSSRNSSIEAFAVCENYSSPEGFNPKDLHRLLEKVGSPSGGDDLDC 240
             ++ FPVVTFAKPKSSRNSSIEAFAVCENYS PEGFNPKDLHRLLEKVGSPSGGDDLDC
Sbjct: 181 --KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDC 240

Query: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300
           SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM
Sbjct: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEM 300

Query: 301 KKASSQGIRELEKLSLDS 319
           KKASSQGIRELEKLS DS
Sbjct: 301 KKASSQGIRELEKLSSDS 316

[Close]

BLAST of PI0003149 vs. TAIR 10
Match: AT5G01230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 566.6 bits (1459), Expect = 1.2e-161
Identity = 279/307 (90.88%), Postives = 294/307 (95.77%), Query Frame = 0


Query: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60
           MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 1   MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 60

Query: 61  RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 120
           R+LYLPAK S + KDGDLPLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKA
Sbjct: 61  RQLYLPAKSSAESKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKA 120

Query: 121 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYSQV 180
           DLVVCDGAPDVTGLHDMDEFVQ QLILAGLTIVTHIL+EGGKFIAKIFRGKDTSLLY Q+
Sbjct: 121 DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHILKEGGKFIAKIFRGKDTSLLYCQL 180

Query: 181 NAEVIFPVVTFAKPKSSRNSSIEAFAVCENYSSPEGFNPKDLHRLLEKVGSPSGGDDLDC 240
             ++ FP VTFAKPKSSRNSSIEAFAVCENYS PEGFNP+DLHRLLEKVGSPSGG DLDC
Sbjct: 181 --KLFFPTVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPRDLHRLLEKVGSPSGGSDLDC 240

Query: 241 SSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEG-TYRSLDPVQPPIAPPYKRALE 300
           SSGWLEGPNKVYIPFLACGDL+GYDSDRSYPLPR A+G +Y+SLDP+QPPIAPPYKRALE
Sbjct: 241 SSGWLEGPNKVYIPFLACGDLTGYDSDRSYPLPREADGSSYQSLDPIQPPIAPPYKRALE 300

Query: 301 MKKASSQ 307
           +KKAS+Q
Sbjct: 301 LKKASAQ 305

[Close]

BLAST of PI0003149 vs. TAIR 10
Match: AT4G25730.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 122.5 bits (306), Expect = 6.2e-28
Identity = 78/242 (32.23%), Postives = 129/242 (53.31%), Query Frame = 0


Query: 1   MGKA-SRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVL 60
           MGK   + + D YYR AKE G+R+R+++KLLQ+D ++++      V+DLCAAPG W QV 
Sbjct: 1   MGKVKGKHRLDKYYRLAKERGFRSRASYKLLQLDAKYSLLHSAHAVLDLCAAPGGWMQVA 60

Query: 61  SRKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDIT----NARTAEVVIRHF 120
             K+ + +            L++ IDL P+ P+ G + +  DIT     ++  +V+ +H 
Sbjct: 61  VEKVPVGS------------LVLGIDLVPILPVRGCVTMTQDITRTECKSKIKQVMEQH- 120

Query: 121 DGCKA-DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKD-T 180
            G  A +LV+ DG+P+V G    +   Q  L++  + + T  L   G  + K+FR +D  
Sbjct: 121 -GVSAFNLVLHDGSPNVGGAWAQEAMSQNALVIDSVRLATEFLARNGNLVTKVFRSRDYN 180

Query: 181 SLLYSQVNAEVIFPVVTFAKPKSSRNSSIEAFAVCENYSSPEGFNPK--DLHRLLEKVGS 234
           S+LY       +F  V   KP +SR++S E + V   Y +P   +P+  D   L ++   
Sbjct: 181 SVLYCLGR---LFEKVEVFKPPASRSASAETYLVGLKYLAPAKIDPRLLDYRHLFKESAE 225

[Close]

BLAST of PI0003149 vs. TAIR 10
Match: AT5G01230.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 120.2 bits (300), Expect = 3.1e-27
Identity = 57/57 (100.00%), Postives = 57/57 (100.00%), Query Frame = 0


Query: 1  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ 58
          MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ
Sbjct: 1  MGKASRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ 57

[Close]

BLAST of PI0003149 vs. TAIR 10
Match: AT5G13830.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 69.7 bits (169), Expect = 4.8e-12
Identity = 54/227 (23.79%), Postives = 103/227 (45.37%), Query Frame = 0


Query: 10  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69
           D +YR+A+  G+ ARSAFKLLQI +++ + +    V+DL  APG+W QV  + L      
Sbjct: 8   DFFYREAQRLGYVARSAFKLLQIQKQYKLIKPGSSVLDLGCAPGAWLQVACQSL------ 67

Query: 70  SPDLKDGDLPLIVAIDLQ----PMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVC 129
              L+ G   ++V +D++    P      V  +  D+ N    ++           +++ 
Sbjct: 68  -GPLRSGG--IVVGMDIKKVKVPPQCDSRVQTIAADVLNFPRQKIRELSPQQLGFSVILS 127

Query: 130 DGAPDVTGLHDMDEFVQFQLILAGLTIVT---------------------HILREGGKFI 189
           D    V+G+   D  +  +L +  L +                        +LR GG  +
Sbjct: 128 DMCHSVSGITTRDAALSAELGMRALDLAVGQAAISQSPNDDDGGPNESRPGVLRHGGHLV 187

Query: 190 AKIFRGKDTSLLYSQVNAEVIFPVVTFAKPKSSRNSSIEAFAVCENY 212
            K+   +D    ++++  + IF   ++ +PK++R SS E + +C+ +
Sbjct: 188 IKLLESEDAQ-DFARI-CKPIFNKASWLRPKATRPSSREIYLICQGF 223

The following BLAST results are available for this feature:

BLAST of PI0003149 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9UET6	4.9e-94	56.95	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapien...	[more]
O36015	2.4e-85	54.15	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosacch...	[more]
Q22031	7.8e-84	53.82	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabdi...	[more]
Q54VA8	3.7e-78	55.07	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Dictyosteli...	[more]
P38238	6.0e-76	50.32	tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Saccharomyces cerevi...	[more]

BLAST of PI0003149 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B6C8	6.9e-176	97.80	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis mel...	[more]
A0A0A0LCV6	1.7e-174	97.17	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis sat...	[more]
A0A6J1F1P7	2.3e-171	95.28	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita m...	[more]
A0A5A7TQN9	5.1e-171	97.73	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis mel...	[more]
A0A6J1IYY9	1.6e-169	94.65	Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita m...	[more]

BLAST of PI0003149 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008442455.1	1.4e-175	97.80	PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase iso...	[more]
XP_004137730.1	3.5e-174	97.17	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativ...	[more]
XP_038903231.1	2.8e-171	95.28	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa his...	[more]
KAG6580486.1	4.7e-171	95.28	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase, partial [Cucu...	[more]
XP_022934133.1	4.7e-171	95.28	putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita mos...	[more]

BLAST of PI0003149 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01230.1	1.2e-161	90.88	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT4G25730.1	6.2e-28	32.23	FtsJ-like methyltransferase family protein	[more]
AT5G01230.2	3.1e-27	100.00	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G13830.1	4.8e-12	23.79	FtsJ-like methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002877	Ribosomal RNA methyltransferase FtsJ domain	PFAM	PF01728	FtsJ	coord: 21..211 e-value: 2.7E-61 score: 206.8
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 20..216 e-value: 2.9E-69 score: 234.7
None	No IPR available	PIRSR	PIRSR005461-1	PIRSR005461-1	coord: 2..212 e-value: 9.9E-66 score: 219.1
None	No IPR available	PANTHER	PTHR10920	RIBOSOMAL RNA METHYLTRANSFERASE	coord: 1..310
None	No IPR available	PANTHER	PTHR10920:SF25	TRNA (CYTIDINE(32)/GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATED	coord: 1..310
IPR015507	Ribosomal RNA large subunit methyltransferase E	HAMAP	MF_01547	RNA_methyltr_E	coord: 5..212 score: 31.187141
IPR028590	tRNA (cytidine(32)/guanosine(34)-2-O)-methyltransferase, Trm7	HAMAP	MF_03162	RNA_methyltr_E_TRM7	coord: 1..270 score: 32.203022
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 20..211

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0003149.1	PI0003149.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002181	cytoplasmic translation
biological_process	GO:0002128	tRNA nucleoside ribose methylation
biological_process	GO:0032259	methylation
biological_process	GO:0001510	RNA methylation
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0008175	tRNA methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity