Homology
BLAST of PI0002984 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 534.3 bits (1375), Expect = 1.5e-150
Identity = 268/510 (52.55%), Postives = 365/510 (71.57%), Query Frame = 0
Query: 1 MEDSILQYSAFSILSIAVVVLAI-IKYWLNFNSNKNLPPSPP-TIPIIGHLHLLKLPVHR 60
ME S L Y+ +AVV+L+I +K + +NLPPSP +P+IGHLHL+ +HR
Sbjct: 1 MESSTLLYT-----FLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHR 60
Query: 61 TLQTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETT 120
+L LS+KYG V SL+ G+RLV+VV+S AAEECFTKND++FANRP F + + YN TT
Sbjct: 61 SLYDLSKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTT 120
Query: 121 LGAAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKP 180
+ +PYG+HWR LRR++ +EI + LN IR DEV++ + +Y++S FGKVE K
Sbjct: 121 MVGSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKS 180
Query: 181 LVKELTMNITMRMVAGKRYFGEEAAKSSEARTFQAIVDELFEFTLSSYPADFLPILKYID 240
+ EL+ N+TMRMVAGKRYFG++ S EA+ F+A++ E+FE +S P DF+P L++ID
Sbjct: 181 KLSELSFNVTMRMVAGKRYFGQD-VDSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWID 240
Query: 241 IQGFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDII 300
+ + K+ K+ +DAF Q LI E R I+ + M+ H L LQE+QPEYYTD II
Sbjct: 241 FKNYEKKVSKISQEMDAFLQRLIHESR---INKNNV--TMIDHLLSLQESQPEYYTDQII 300
Query: 301 KGLILTMILGGSDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSY 360
KG+I+ ++L G+DT+AVT+EWAMS LLNHP LEKA+ EIE+Q+G ++L+EE DL KL+Y
Sbjct: 301 KGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTY 360
Query: 361 LRCIVLETLRLYPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSF 420
L I+ ET RL PA P+L+PH+SS +C + GYDVP+ TILLVNAWAIHRDP W++PT F
Sbjct: 361 LHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLF 420
Query: 421 RPERFENNNGEGDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEV 480
+PER +G + ++ FG+GRR+CPG+G+A RV+GL LG LIQCF+WK I ++
Sbjct: 421 KPER----HGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKI 480
Query: 481 DMNEGKGVSMPKAQPLEALCKAREIATKLL 509
DM EG G++MPKAQPLEALCK R I K++
Sbjct: 481 DMAEGSGLTMPKAQPLEALCKPRNILHKVV 495
BLAST of PI0002984 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 530.0 bits (1364), Expect = 2.9e-149
Identity = 257/491 (52.34%), Postives = 350/491 (71.28%), Query Frame = 0
Query: 13 ILSIAVVVLAIIKYWLNFNSNKNLPPSPPTIPIIGHLHLLKLPVHRTLQTLSQKYGPVLS 72
I+S+ +++ + ++ KNLPP P +PIIG+LH LK P+H T TLSQKYG + S
Sbjct: 9 IISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFS 68
Query: 73 LRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLGAAPYGDHWRKLR 132
L FGSRLVVVV+SL A+ECFTKND++ ANRP+F + YN TT+ +PYGDHWR LR
Sbjct: 69 LWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLR 128
Query: 133 RVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKPLVKELTMNITMRMV 192
R+ ++EIL S RLN+ EIRRDE+ R ++K+ + S +GF +VE +P+ E+T N MRMV
Sbjct: 129 RILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRMV 188
Query: 193 AGKRYFGEEAAKS--SEARTFQAIVDELFEFTLSSYPADFLPILKYIDIQGFMKRAKKLI 252
+GKRY+G + S EAR F+ I+ E+ ++ DFL L++ D G KR KK+
Sbjct: 189 SGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKIS 248
Query: 253 TRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIKGLILTMILGGS 312
R DAF QGLIDEHR G+ ++ N M+ H L Q++QPEYYTD IIKGL++ M+L G+
Sbjct: 249 KRTDAFLQGLIDEHRFGKRNS----NTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGT 308
Query: 313 DTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSYLRCIVLETLRLY 372
DT++VTIEWAMSNLLNHP +++KAK E+++ +G D+ ++E D+SKL YL+ IV ETLRL+
Sbjct: 309 DTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLH 368
Query: 373 PAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFENNNGEG 432
A PLL+PH SS + S+ GY++P++TIL+VNAW IHRDP LW DPT F+PERFE EG
Sbjct: 369 AAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEK---EG 428
Query: 433 DGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVDMNEGKGVSMPK 492
+ N +++FGLGRRACPG ++ R GL LG+LIQCF+WK I +++DM E KG++ K
Sbjct: 429 EVNK--LLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAGK 488
Query: 493 AQPLEALCKAR 502
L A+CK R
Sbjct: 489 KTSLNAMCKVR 490
BLAST of PI0002984 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 525.8 bits (1353), Expect = 5.5e-148
Identity = 261/499 (52.30%), Postives = 353/499 (70.74%), Query Frame = 0
Query: 11 FSILSIAVVVLAIIKYWLNFNSNKNLPPSPPTIPIIGHLHLLKLPVHRTLQTLSQKYGPV 70
+S+LS++ ++ I + KNLPP PPTIPIIG+LH LK P+HRT TLSQ YG +
Sbjct: 5 YSLLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDI 64
Query: 71 LSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLGAAPYGDHWRK 130
SL FGSRLVVVV+S A ECFTKND+I ANRP F + YN TTLG+A YGDHWR
Sbjct: 65 FSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRN 124
Query: 131 LRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIY-EASRDGFG--KVEFKPLVKELTMNI 190
LRR++T+++L ++RLN+ +RRDE R ++K+ + +GFG KVE +P + E+T N
Sbjct: 125 LRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNA 184
Query: 191 TMRMVAGKRYFGEEAAKS--SEARTFQAIVDELFEFTLSSYPADFLPILKYIDIQGFMKR 250
MRM++GKRY+G++ S EA+ F+ I+ E+ ++ DFLP+L+ +D+ KR
Sbjct: 185 MMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKR 244
Query: 251 AKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIKGLILTM 310
K++ R +AF +GLI+EHRRG I ++ M+ H LKL E+QPEYY+D +IKGLI M
Sbjct: 245 CKRIAKRSNAFLEGLIEEHRRGNIHSD--GGTMIDHLLKLSESQPEYYSDHLIKGLIQGM 304
Query: 311 ILGGSDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSYLRCIVLE 370
+L G+DT+AVTIEW MS LLNHP VL+KAK E+++Q+G +KL++E DLSKL YL+ I+ E
Sbjct: 305 LLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISE 364
Query: 371 TLRLYPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEN 430
TLRL+P PLLLPH SS +C+I ++VP+DTI+L N W IHRDP W D SF+PERFE
Sbjct: 365 TLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEK 424
Query: 431 NNGEGDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVDMNEGKG 490
+ NK+M AFGLGRRACPG +A R +G +G+LIQCF+W+ +++DM EGKG
Sbjct: 425 E----EEVNKVM-AFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKG 484
Query: 491 VSMPKAQPLEALCKAREIA 505
++MP PL A+CKA IA
Sbjct: 485 ITMPMKIPLRAMCKALPIA 496
BLAST of PI0002984 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 510.8 bits (1314), Expect = 1.8e-143
Identity = 249/508 (49.02%), Postives = 355/508 (69.88%), Query Frame = 0
Query: 4 SILQYSAF--SILSIAVVVLAIIKYWLNFNSNKNLPPSPPTIPIIGHLHLLKLPVHRTLQ 63
S+L YS F ++ I +V+ K+ KNLPP PP++PIIG+LH LK P+HRT +
Sbjct: 5 SLLSYSVFYLALFFIFNIVIRARKF-------KNLPPGPPSLPIIGNLHHLKRPLHRTFK 64
Query: 64 TLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLGA 123
LS+KYG V SL FGSRLVVVV+S ++CFTKNDV+ ANRP F + YN TTLG+
Sbjct: 65 GLSEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGS 124
Query: 124 APYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKPLVK 183
YG+HWR LRR++ L++L + R+N+ IRRDE +R + ++ + S F ++E +
Sbjct: 125 TSYGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLY 184
Query: 184 ELTMNITMRMVAGKRYFGEEAAKS--SEARTFQAIVDELFEFTLSSYPADFLPILKYIDI 243
++T N MRM++GKRY+GE+ S EA F+ +V EL + + ++ DF+P+L+++D
Sbjct: 185 DMTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDF 244
Query: 244 QGFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIK 303
+ KR K + + DAF +GLI+EHR + +E N M+ H L LQ++QPEYYTD IIK
Sbjct: 245 ENLEKRLKDISGKTDAFLRGLIEEHR----TKKERANTMIDHLLNLQDSQPEYYTDQIIK 304
Query: 304 GLILTMILGGSDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSYL 363
GL L M+L G+D++AVT+EW+MSNLLNHP VL+K K E+++ +G D+L++E+DL KL+YL
Sbjct: 305 GLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYL 364
Query: 364 RCIVLETLRLYPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFR 423
+ ++ ETLRLY PLLLPH +S C+I GY VP+DTI+L+NAWAIHRDP LW + T+F+
Sbjct: 365 KNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFK 424
Query: 424 PERFENNNGEGDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVD 483
PERFE G + +IAFG+GRRACPG G+A R + + L +LIQCFDWK I+G ++D
Sbjct: 425 PERFEKK-----GELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKID 484
Query: 484 MNEGKGVSMPKAQPLEALCKAREIATKL 508
+ E G ++ K PL+A+CK+R + K+
Sbjct: 485 LAERDGFTLTKLVPLKAMCKSRPVINKV 496
BLAST of PI0002984 vs. ExPASy Swiss-Prot
Match:
Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)
HSP 1 Score: 503.1 bits (1294), Expect = 3.8e-141
Identity = 243/505 (48.12%), Postives = 349/505 (69.11%), Query Frame = 0
Query: 5 ILQYSAFSILSIAVVVLAIIKYWLNFNSNKNLPPSPPTIPIIGHLHLLKLPVHRTLQTLS 64
IL Y +S+ ++ + II+ KNLPP P ++PIIG+LH LK P++RT + L+
Sbjct: 3 ILSYLCYSLFYLS--IFFIIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALT 62
Query: 65 QKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLGAAPY 124
+KYG V+SL FGSRLVVVV+SL +ECFTKNDV+ ANRP F + YN TTLG+ Y
Sbjct: 63 EKYGNVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSY 122
Query: 125 GDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKPLVKELT 184
G+HWR LRR+++L++L + R+N IRRDE +R +KK+ E S F +VE ++T
Sbjct: 123 GEHWRNLRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMT 182
Query: 185 MNITMRMVAGKRYFGEEA--AKSSEARTFQAIVDELFEFTLSSYPADFLPILKYIDIQGF 244
N MRM++GKRY+G++ ++ EA F+ +V EL + + ++ DF+P+LK++D +
Sbjct: 183 FNNIMRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENL 242
Query: 245 MKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIKGLI 304
KR K++ + D F GL+ E R S +E N M+ H L +QE+QPEYYTD IIKGL
Sbjct: 243 EKRVKRIGEKNDVFLSGLLQEQR----SKKERTNTMIDHLLNMQESQPEYYTDTIIKGLC 302
Query: 305 LTMILGGSDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSYLRCI 364
L M+L G+D++AVT+EW MSN+LN+P VL+K + E+++ +G D+L++E+DL KL+YLR +
Sbjct: 303 LAMLLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNV 362
Query: 365 VLETLRLYPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPER 424
+ ETLRLY PLLLPH ++ C + GY VPRDTI+L+NAWAIHRDP W + T+F+PER
Sbjct: 363 IYETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPER 422
Query: 425 FENNNGEGDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVDMNE 484
F+ G + MIAFG+GRRACPG G+A R + + L +L+QCFDWK I+ +++DM+E
Sbjct: 423 FDKK-----GELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSE 482
Query: 485 GKGVSMPKAQPLEALCKAREIATKL 508
G +M K PL+A+CK R + K+
Sbjct: 483 RDGFTMTKLLPLKAMCKTRPVVNKV 496
BLAST of PI0002984 vs. ExPASy TrEMBL
Match:
A0A5D3DLU7 (Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001700 PE=3 SV=1)
HSP 1 Score: 984.2 bits (2543), Expect = 2.1e-283
Identity = 489/508 (96.26%), Postives = 499/508 (98.23%), Query Frame = 0
Query: 1 MEDSILQYSAFSILSIAVVVLAIIKYWLNFNSNKNLPPSPPTIPIIGHLHLLKLPVHRTL 60
MEDSILQYSAFSILSI VVVLAIIKY L FNSNKNLPPSPP+IPIIGHLHLLKLPVHRTL
Sbjct: 1 MEDSILQYSAFSILSIGVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60
Query: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
Query: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKPLV 180
AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRA+KKI+E SRDGFGKVEFKPLV
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAVKKIFEVSRDGFGKVEFKPLV 180
Query: 181 KELTMNITMRMVAGKRYFGEEAAKSSEARTFQAIVDELFEFTLSSYPADFLPILKYIDIQ 240
KELTMNITMRMVAGKRY+GEEAAKSSEARTFQAIV ELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 181 KELTMNITMRMVAGKRYYGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240
Query: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIKG 300
GFMKRAKKLI RVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQP+YYTDDIIKG
Sbjct: 241 GFMKRAKKLIARVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300
Query: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSYLR 360
LILTMILGGSDTTAVTIEWAMSNLLNHPSVL+KA+AEIESQL GDKLLEETDLSKLSYLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLDGDKLLEETDLSKLSYLR 360
Query: 361 CIVLETLRLYPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
CIVLETLRLYPAGPLLLPHKSSA+CSISGY++PRDTILLVNAWAIHRDPTLWEDPTSFRP
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCSISGYNIPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
Query: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVDM 480
ERFENNNGEGD NNKLMIAFGLGRRACPGTGMAHRVMGLMLG LIQCFDWKSIDGKEVDM
Sbjct: 421 ERFENNNGEGDANNKLMIAFGLGRRACPGTGMAHRVMGLMLGTLIQCFDWKSIDGKEVDM 480
Query: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 509
NEGKGVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508
BLAST of PI0002984 vs. ExPASy TrEMBL
Match:
A0A1S3CAW8 (isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103498910 PE=3 SV=1)
HSP 1 Score: 984.2 bits (2543), Expect = 2.1e-283
Identity = 489/508 (96.26%), Postives = 499/508 (98.23%), Query Frame = 0
Query: 1 MEDSILQYSAFSILSIAVVVLAIIKYWLNFNSNKNLPPSPPTIPIIGHLHLLKLPVHRTL 60
MEDSILQYSAFSILSI VVVLAIIKY L FNSNKNLPPSPP+IPIIGHLHLLKLPVHRTL
Sbjct: 1 MEDSILQYSAFSILSIGVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60
Query: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
Query: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKPLV 180
AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRA+KKI+E SRDGFGKVEFKPLV
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAVKKIFEVSRDGFGKVEFKPLV 180
Query: 181 KELTMNITMRMVAGKRYFGEEAAKSSEARTFQAIVDELFEFTLSSYPADFLPILKYIDIQ 240
KELTMNITMRMVAGKRY+GEEAAKSSEARTFQAIV ELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 181 KELTMNITMRMVAGKRYYGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240
Query: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIKG 300
GFMKRAKKLI RVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQP+YYTDDIIKG
Sbjct: 241 GFMKRAKKLIARVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300
Query: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSYLR 360
LILTMILGGSDTTAVTIEWAMSNLLNHPSVL+KA+AEIESQL GDKLLEETDLSKLSYLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLDGDKLLEETDLSKLSYLR 360
Query: 361 CIVLETLRLYPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
CIVLETLRLYPAGPLLLPHKSSA+CSISGY++PRDTILLVNAWAIHRDPTLWEDPTSFRP
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCSISGYNIPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
Query: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVDM 480
ERFENNNGEGD NNKLMIAFGLGRRACPGTGMAHRVMGLMLG LIQCFDWKSIDGKEVDM
Sbjct: 421 ERFENNNGEGDANNKLMIAFGLGRRACPGTGMAHRVMGLMLGTLIQCFDWKSIDGKEVDM 480
Query: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 509
NEGKGVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508
BLAST of PI0002984 vs. ExPASy TrEMBL
Match:
A0A097IYP1 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa6G088160 PE=2 SV=1)
HSP 1 Score: 978.0 bits (2527), Expect = 1.5e-281
Identity = 484/508 (95.28%), Postives = 498/508 (98.03%), Query Frame = 0
Query: 1 MEDSILQYSAFSILSIAVVVLAIIKYWLNFNSNKNLPPSPPTIPIIGHLHLLKLPVHRTL 60
MEDSILQYSAFSILSIAVVVLAIIKY L FNSNKNLPPSPP+IPIIGHLHLLKLPVHRTL
Sbjct: 1 MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60
Query: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
QTLSQKYGPVLSLRFGSR VVVVTSLEAAEECF KND+IFANRPNFAVSHCLSYNETTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 120
Query: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKPLV 180
AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYE SRDGFGKVEFKPLV
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 180
Query: 181 KELTMNITMRMVAGKRYFGEEAAKSSEARTFQAIVDELFEFTLSSYPADFLPILKYIDIQ 240
KELTMN+TMRMVAGKRYFGEEAAKSSEARTFQAIV ELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 181 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240
Query: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIKG 300
GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQP+YYTDDIIKG
Sbjct: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300
Query: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSYLR 360
LILTMILGGSDTTAVTIEWAMSNLLNHPSVL+KA+AEIESQLGGDKL+EETDLSKL+YLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 360
Query: 361 CIVLETLRLYPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
I+LETLRLYPAGPLLLPHKSSA+C ISGYD+PRDT+LLVNAWAIHRDP LWEDPTSF+P
Sbjct: 361 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 420
Query: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVDM 480
ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMA+RVMGLMLG LIQCFDWKSIDGKEVDM
Sbjct: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 480
Query: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 509
NEGKGVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508
BLAST of PI0002984 vs. ExPASy TrEMBL
Match:
A0A6J1H614 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111460794 PE=3 SV=1)
HSP 1 Score: 817.4 bits (2110), Expect = 3.4e-233
Identity = 406/508 (79.92%), Postives = 456/508 (89.76%), Query Frame = 0
Query: 1 MEDSILQYSAFSILSIAVVVLAIIKYWLNFNSNKNLPPSPPTIPIIGHLHLLKLPVHRTL 60
M+DS YSA L IA+VV I Y L S+KNLPP+PP+IPIIGHLHLLK PVHR+L
Sbjct: 1 MDDSTFLYSA---LCIALVV--AISYALKPKSHKNLPPTPPSIPIIGHLHLLKPPVHRSL 60
Query: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
TLSQKYGPVLSLRFGSRLVVVV+S++AAEECFTKNDV+FANRP+FAVS CLSYN TTLG
Sbjct: 61 LTLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLG 120
Query: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKPLV 180
AAPYGDHWRKLRRVS LEIL ++RLN N IRRDEVRR K+ + SRD F +VEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLANKLSQTSRDDFARVEFKSMV 180
Query: 181 KELTMNITMRMVAGKRYFGEEAAKSSEARTFQAIVDELFEFTLSSYPADFLPILKYIDIQ 240
KELT+NITMRMVAGKRY+GEE +SSEA TF+AIVDELFEF L+ YPADFLPILKYIDIQ
Sbjct: 181 KELTLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDIQ 240
Query: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIKG 300
GFMKRAKKLI RVDAFWQG++DEHR +I N+EM+N MVAHFLKLQE+QP+YYTDDIIKG
Sbjct: 241 GFMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKG 300
Query: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSYLR 360
LILTM+LGGSDTTAVTIEWAMSNLLNHP+VL+KA+AE+E+QL GD+LLEE+++ KLSYLR
Sbjct: 301 LILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQL-GDQLLEESEVPKLSYLR 360
Query: 361 CIVLETLRLYPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
CIVLETLRLYPAGPLLLPHKSSA+C++SGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
Query: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVDM 480
ERFE G+G+ N+KLMIAFGLGRRACPG+GMAHRV+GL LG+LIQCFDWKSIDGKEVDM
Sbjct: 421 ERFE--GGDGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDM 480
Query: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 509
EGKGVSMPKA+PLEALCK R IA+KLL
Sbjct: 481 TEGKGVSMPKAEPLEALCKPRPIASKLL 500
BLAST of PI0002984 vs. ExPASy TrEMBL
Match:
A0A6J1KS03 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111498126 PE=3 SV=1)
HSP 1 Score: 811.6 bits (2095), Expect = 1.8e-231
Identity = 401/508 (78.94%), Postives = 456/508 (89.76%), Query Frame = 0
Query: 1 MEDSILQYSAFSILSIAVVVLAIIKYWLNFNSNKNLPPSPPTIPIIGHLHLLKLPVHRTL 60
M+DS Y S+L IA+VV I Y L S KNLPP+PP+IPIIGHLHLLK PVHR+L
Sbjct: 1 MDDSTFLY---SVLCIALVV--AISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSL 60
Query: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
TLSQKYGPVLSLRFGSRLVVVV+S++AAEECFTKNDV+FANRP+FAVS CLSYN TTLG
Sbjct: 61 LTLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLG 120
Query: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKPLV 180
AAPYGDHWRKLRRVS LEIL ++RLN N IRRDEVRR + K+ ++SRD F +VEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMV 180
Query: 181 KELTMNITMRMVAGKRYFGEEAAKSSEARTFQAIVDELFEFTLSSYPADFLPILKYIDIQ 240
KELT+NITMRMVAGKRY+GEE +SSEARTF+AIV+ELFEF L+ YPADFLPILKYID Q
Sbjct: 181 KELTLNITMRMVAGKRYYGEETNESSEARTFRAIVNELFEFMLAQYPADFLPILKYIDFQ 240
Query: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIKG 300
GFMKRAKKLI RVDAFWQG++DEHR ++ N+EM+N MVAHFLKLQE+QP+YYTDDIIKG
Sbjct: 241 GFMKRAKKLIKRVDAFWQGIVDEHRTNQVHNQEMENSMVAHFLKLQESQPQYYTDDIIKG 300
Query: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSYLR 360
LILTM+LGGSDTTAVTIEWAMSNLLNHP+VL+KA+AE+E+QL GD+LLEE+++ KLSYLR
Sbjct: 301 LILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQL-GDQLLEESEVPKLSYLR 360
Query: 361 CIVLETLRLYPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
CIVLETLRLYPAGPLLLPHKSSA+C++SGY+VPRDTILLVNAWAIHRDPTLWEDPTSFRP
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCTLSGYNVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
Query: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVDM 480
ERFE G+ + N+KLMIAFGLGRRACPG+GMAHRV+GL LG+LIQCFDWKSIDGKEVDM
Sbjct: 421 ERFE--GGDSEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDM 480
Query: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 509
EGKGVSMPKA+PLEALCK R IA+KLL
Sbjct: 481 TEGKGVSMPKAEPLEALCKPRPIASKLL 500
BLAST of PI0002984 vs. NCBI nr
Match:
XP_008459942.1 (PREDICTED: isoflavone 3'-hydroxylase-like [Cucumis melo] >KAA0039878.1 isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa] >TYK24623.1 isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa])
HSP 1 Score: 984.2 bits (2543), Expect = 4.3e-283
Identity = 489/508 (96.26%), Postives = 499/508 (98.23%), Query Frame = 0
Query: 1 MEDSILQYSAFSILSIAVVVLAIIKYWLNFNSNKNLPPSPPTIPIIGHLHLLKLPVHRTL 60
MEDSILQYSAFSILSI VVVLAIIKY L FNSNKNLPPSPP+IPIIGHLHLLKLPVHRTL
Sbjct: 1 MEDSILQYSAFSILSIGVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60
Query: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
Query: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKPLV 180
AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRA+KKI+E SRDGFGKVEFKPLV
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAVKKIFEVSRDGFGKVEFKPLV 180
Query: 181 KELTMNITMRMVAGKRYFGEEAAKSSEARTFQAIVDELFEFTLSSYPADFLPILKYIDIQ 240
KELTMNITMRMVAGKRY+GEEAAKSSEARTFQAIV ELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 181 KELTMNITMRMVAGKRYYGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240
Query: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIKG 300
GFMKRAKKLI RVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQP+YYTDDIIKG
Sbjct: 241 GFMKRAKKLIARVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300
Query: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSYLR 360
LILTMILGGSDTTAVTIEWAMSNLLNHPSVL+KA+AEIESQL GDKLLEETDLSKLSYLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLDGDKLLEETDLSKLSYLR 360
Query: 361 CIVLETLRLYPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
CIVLETLRLYPAGPLLLPHKSSA+CSISGY++PRDTILLVNAWAIHRDPTLWEDPTSFRP
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCSISGYNIPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
Query: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVDM 480
ERFENNNGEGD NNKLMIAFGLGRRACPGTGMAHRVMGLMLG LIQCFDWKSIDGKEVDM
Sbjct: 421 ERFENNNGEGDANNKLMIAFGLGRRACPGTGMAHRVMGLMLGTLIQCFDWKSIDGKEVDM 480
Query: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 509
NEGKGVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508
BLAST of PI0002984 vs. NCBI nr
Match:
NP_001289568.1 (isoflavone 2'-hydroxylase-like [Cucumis sativus] >AIT72031.1 cytochrome P450 [Cucumis sativus])
HSP 1 Score: 978.0 bits (2527), Expect = 3.1e-281
Identity = 484/508 (95.28%), Postives = 498/508 (98.03%), Query Frame = 0
Query: 1 MEDSILQYSAFSILSIAVVVLAIIKYWLNFNSNKNLPPSPPTIPIIGHLHLLKLPVHRTL 60
MEDSILQYSAFSILSIAVVVLAIIKY L FNSNKNLPPSPP+IPIIGHLHLLKLPVHRTL
Sbjct: 1 MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60
Query: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
QTLSQKYGPVLSLRFGSR VVVVTSLEAAEECF KND+IFANRPNFAVSHCLSYNETTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 120
Query: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKPLV 180
AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYE SRDGFGKVEFKPLV
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 180
Query: 181 KELTMNITMRMVAGKRYFGEEAAKSSEARTFQAIVDELFEFTLSSYPADFLPILKYIDIQ 240
KELTMN+TMRMVAGKRYFGEEAAKSSEARTFQAIV ELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 181 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240
Query: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIKG 300
GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQP+YYTDDIIKG
Sbjct: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300
Query: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSYLR 360
LILTMILGGSDTTAVTIEWAMSNLLNHPSVL+KA+AEIESQLGGDKL+EETDLSKL+YLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 360
Query: 361 CIVLETLRLYPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
I+LETLRLYPAGPLLLPHKSSA+C ISGYD+PRDT+LLVNAWAIHRDP LWEDPTSF+P
Sbjct: 361 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 420
Query: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVDM 480
ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMA+RVMGLMLG LIQCFDWKSIDGKEVDM
Sbjct: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 480
Query: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 509
NEGKGVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508
BLAST of PI0002984 vs. NCBI nr
Match:
KGN46394.2 (hypothetical protein Csa_005702 [Cucumis sativus])
HSP 1 Score: 978.0 bits (2527), Expect = 3.1e-281
Identity = 484/508 (95.28%), Postives = 498/508 (98.03%), Query Frame = 0
Query: 1 MEDSILQYSAFSILSIAVVVLAIIKYWLNFNSNKNLPPSPPTIPIIGHLHLLKLPVHRTL 60
MEDSILQYSAFSILSIAVVVLAIIKY L FNSNKNLPPSPP+IPIIGHLHLLKLPVHRTL
Sbjct: 22 MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 81
Query: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
QTLSQKYGPVLSLRFGSR VVVVTSLEAAEECF KND+IFANRPNFAVSHCLSYNETTLG
Sbjct: 82 QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 141
Query: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKPLV 180
AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYE SRDGFGKVEFKPLV
Sbjct: 142 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 201
Query: 181 KELTMNITMRMVAGKRYFGEEAAKSSEARTFQAIVDELFEFTLSSYPADFLPILKYIDIQ 240
KELTMN+TMRMVAGKRYFGEEAAKSSEARTFQAIV ELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 202 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 261
Query: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIKG 300
GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQP+YYTDDIIKG
Sbjct: 262 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 321
Query: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSYLR 360
LILTMILGGSDTTAVTIEWAMSNLLNHPSVL+KA+AEIESQLGGDKL+EETDLSKL+YLR
Sbjct: 322 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 381
Query: 361 CIVLETLRLYPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
I+LETLRLYPAGPLLLPHKSSA+C ISGYD+PRDT+LLVNAWAIHRDP LWEDPTSF+P
Sbjct: 382 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 441
Query: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVDM 480
ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMA+RVMGLMLG LIQCFDWKSIDGKEVDM
Sbjct: 442 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 501
Query: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 509
NEGKGVSMPKAQPLEALCKAREIATKLL
Sbjct: 502 NEGKGVSMPKAQPLEALCKAREIATKLL 529
BLAST of PI0002984 vs. NCBI nr
Match:
XP_038907094.1 (cytochrome P450 81Q32-like [Benincasa hispida])
HSP 1 Score: 905.6 bits (2339), Expect = 1.9e-259
Identity = 451/508 (88.78%), Postives = 479/508 (94.29%), Query Frame = 0
Query: 1 MEDSILQYSAFSILSIAVVVLAIIKYWLNFNSNKNLPPSPPTIPIIGHLHLLKLPVHRTL 60
MEDSIL YSA SILSI +VL IIK+ L N KNLPP PP+IPIIGHLHLLKLPVHRTL
Sbjct: 1 MEDSILAYSAISILSIG-IVLGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTL 60
Query: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
QTLSQKYGPVLSLRFGSRLVVVV+S++AAEECFTKND+IFANRP+FAVS CLSYN TTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLG 120
Query: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKPLV 180
AAPYGDHWRKLRRVS LEIL S+RLN N IRRDEVRR M+KIYEASRD FGKVEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMV 180
Query: 181 KELTMNITMRMVAGKRYFGEEAAKSSEARTFQAIVDELFEFTLSSYPADFLPILKYIDIQ 240
KELT+NITMRMVAGKRY+GEEAAKSSEARTFQAIV+ELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 181 KELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQ 240
Query: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIKG 300
GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEM+N MVAHFLKLQE+QPEYYTDDIIKG
Sbjct: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKG 300
Query: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSYLR 360
LILTMILGGSDTTAVTIEWAMSNLLNHPSVL+KA+AE+ESQLG D+LLEETDLSKLSYLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLR 360
Query: 361 CIVLETLRLYPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
CIVLETLRLYPAGPLLLPHKSSA+C+I GYDVP DTILLVNAWAIHRDPTLWEDP SF+P
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKP 420
Query: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVDM 480
ERFENNNGEGDG++KLMIAFGLGRRACPGTGMAHRV+GL LG+LIQCFDWKSI+GKEVDM
Sbjct: 421 ERFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDM 480
Query: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 509
EG GVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 TEGIGVSMPKAQPLEALCKAREIATKLL 507
BLAST of PI0002984 vs. NCBI nr
Match:
KAG7025244.1 (Isoflavone 2'-hydroxylase [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 819.7 bits (2116), Expect = 1.4e-233
Identity = 406/508 (79.92%), Postives = 457/508 (89.96%), Query Frame = 0
Query: 1 MEDSILQYSAFSILSIAVVVLAIIKYWLNFNSNKNLPPSPPTIPIIGHLHLLKLPVHRTL 60
M+DS Y S+L IA+VV I Y L S KNLPP+PP+IPIIGHLHLLK PVHR+L
Sbjct: 1 MDDSTFLY---SVLCIALVV--AISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSL 60
Query: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
TLSQKYGPVLSLRFGSRLVVVV+S++AAEECFTKNDV+FANRP+FAVS CLSYN TTLG
Sbjct: 61 LTLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLG 120
Query: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKPLV 180
AAPYGDHWRKLRRVS LEIL ++RLN N IRRDEVRR + K+ + SRD F +VEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMV 180
Query: 181 KELTMNITMRMVAGKRYFGEEAAKSSEARTFQAIVDELFEFTLSSYPADFLPILKYIDIQ 240
KELT+NITMRMVAGKRY+GEE +SSEARTF+AIVDELFEF L+ YPADFLPILKYIDIQ
Sbjct: 181 KELTLNITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQ 240
Query: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIKG 300
GFMKRAKKLI RVDAFWQG++DEHR +I N+EM+N MVAHFLKLQE+QP+YYTDDIIKG
Sbjct: 241 GFMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKG 300
Query: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSYLR 360
LILTM+LGGSDTTAVTIEWAMSNLLNHP+VL+KA+AE+E+QL GD+LLEE+++ KLSYLR
Sbjct: 301 LILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQL-GDQLLEESEVPKLSYLR 360
Query: 361 CIVLETLRLYPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
CIVLETLRLYPAGPLLLPHKSSA+C++SGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
Query: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVDM 480
ERFE G+G+ N+KLMIAFGLGRRACPG+GMAHRV+GL LG+LIQCFDWKSIDGKEVDM
Sbjct: 421 ERFE--GGDGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDM 480
Query: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 509
EGKGVSMPKA+PLEALCK R IA+KLL
Sbjct: 481 TEGKGVSMPKAEPLEALCKPRPIASKLL 500
BLAST of PI0002984 vs. TAIR 10
Match:
AT4G37310.1 (cytochrome P450, family 81, subfamily H, polypeptide 1 )
HSP 1 Score: 525.4 bits (1352), Expect = 5.1e-149
Identity = 260/503 (51.69%), Postives = 361/503 (71.77%), Query Frame = 0
Query: 13 ILSIAVVVLAIIKYWLNFNSNKNLPPSPP-TIPIIGHLHLLKLP-VHRTLQTLSQKYGPV 72
IL+ V + + L NKNLPPSP PIIGHLHLLK P +HRTL LS GPV
Sbjct: 7 ILTTLVAIFIVKIVLLVTKPNKNLPPSPNICFPIIGHLHLLKKPLLHRTLSHLSHSLGPV 66
Query: 73 LSLRFGSRLVVVVTSLEAAEECF-TKNDVIFANRPNFAVSHCLSYNETTLGAAPYGDHWR 132
SLR GSRL V+++S AAEECF TKND++ ANRP F + ++Y+ T++ APYGDHWR
Sbjct: 67 FSLRLGSRLAVIISSPTAAEECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAPYGDHWR 126
Query: 133 KLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKPLVKELTMNITM 192
LRR++ LE+ ++RLNA+ EIR DEV+ ++K+++ S + KVE + L+ LT+N+ M
Sbjct: 127 NLRRITALEVFSTNRLNASAEIRHDEVKMLLQKLHDLSVERPAKVELRQLLTGLTLNVIM 186
Query: 193 RMVAGKRYFGE-EAAKSSEARTFQAIVDELFEFTLSSYPADFLPILKYIDIQGFMKRAKK 252
RM+ GKR+F E + K+ + F+ +V E+ E + + PADFLP L++ D +G +KRAK+
Sbjct: 187 RMMTGKRFFEEDDGGKAGISLEFRELVAEILELSAADNPADFLPALRWFDYKGLVKRAKR 246
Query: 253 LITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIKGLILTMILG 312
+ R+D+ QG +DEHR + E KN M+AH L QE +P Y+D IKGLIL M++G
Sbjct: 247 IGERMDSLLQGFLDEHRANK-DRLEFKNTMIAHLLDSQEKEPHNYSDQTIKGLILMMVVG 306
Query: 313 GSDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQL---GGDKLLEETDLSKLSYLRCIVLE 372
G+DT+A+T+EWAMSNLLNHP +LE + I++Q+ +LL+E DL ++YL+ +V E
Sbjct: 307 GTDTSALTVEWAMSNLLNHPQILETTRQNIDTQMETSSSRRLLKEEDLVNMNYLKNVVSE 366
Query: 373 TLRLYPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFEN 432
TLRLYP PL++PH S++C I G++VPRDTI+LVN WAIHRDP++W+DPTSF+PERFE
Sbjct: 367 TLRLYPVAPLMVPHVPSSDCVIGGFNVPRDTIVLVNLWAIHRDPSVWDDPTSFKPERFEG 426
Query: 433 NNGEGDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVDMNEGKG 492
++ G N K+M FGLGRRACPG +A+RV+GL+LG +IQCF+W+S G +VDM EG G
Sbjct: 427 SDQFGHYNGKMM-PFGLGRRACPGLSLANRVVGLLLGSMIQCFEWESGSGGQVDMTEGPG 486
Query: 493 VSMPKAQPLEALCKAREIATKLL 509
+S+PKA+PL C+ RE+A++LL
Sbjct: 487 LSLPKAEPLVVTCRTREMASELL 507
BLAST of PI0002984 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 509.6 bits (1311), Expect = 2.9e-144
Identity = 255/498 (51.20%), Postives = 350/498 (70.28%), Query Frame = 0
Query: 13 ILSIAVVVLAIIKYWLNFNSNKNLPPSPP-TIPIIGHLHLLKLPVHRTLQTLSQKY--GP 72
I SI VVL++I NLPPSP ++P+IGHL LLK P+HRT +LSQ P
Sbjct: 7 IFSILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAP 66
Query: 73 VLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLGAAPYGDHWR 132
+ SLR G+RLV V +S AEECFTKNDV+ ANRPNF ++ ++Y+ TT+ AA YGDHWR
Sbjct: 67 IFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWR 126
Query: 133 KLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKPLVKELTMNITM 192
LRR+ ++EI + RLN+ IR+DE+RR + ++ F KV+ K ++ +LT N +
Sbjct: 127 NLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNIL 186
Query: 193 RMVAGKRYFGEEAAKSSEARTFQAIVDELFEFTLSSYPADFLPILKYIDIQGFMKRAKKL 252
RMVAGKRY+G+ EA+ + ++ ++ + D+LP+L+ + + R KKL
Sbjct: 187 RMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRL--VSDYETRVKKL 246
Query: 253 ITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIKGLILTMILGG 312
R+D F QGL+DE R +E N M+ H L LQE+QP+Y+TD IIKG +L +IL G
Sbjct: 247 AGRLDEFLQGLVDEKREA----KEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAG 306
Query: 313 SDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSYLRCIVLETLRL 372
+DT+AVT+EWA+SN+LNHP VL KA+ EI+ ++G D+L++E+D+S L YL+ IV ETLRL
Sbjct: 307 TDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRL 366
Query: 373 YPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFENNNGE 432
YPA P+LLPH +S +C ++GYD+PR TILL N WAIHRDP LW+DP SF+PERFE
Sbjct: 367 YPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEK---- 426
Query: 433 GDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVDMNEGKGVSMP 492
+G + ++ FGLGRRACPG+G+AHR++ L LG LIQC +W+ I G+EVDM+EGKGV+MP
Sbjct: 427 -EGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKI-GEEVDMSEGKGVTMP 486
Query: 493 KAQPLEALCKAREIATKL 508
KA+PLEA+C+AR K+
Sbjct: 487 KAKPLEAMCRARPSVVKI 492
BLAST of PI0002984 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 503.1 bits (1294), Expect = 2.7e-142
Identity = 257/499 (51.50%), Postives = 343/499 (68.74%), Query Frame = 0
Query: 13 ILSIAVVVLAIIKYWLNFNSNKNLPPSPP-TIPIIGHLHLLKLPVHRTLQTLSQKYG--P 72
I + +VL++I NLPPSP +P+IGHL LLK P+HR ++SQ G P
Sbjct: 7 IFTFCFIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAP 66
Query: 73 VLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLGAAPYGDHWR 132
++SLR G+RL+ VV+S AEECFTKNDVI ANR + +SY +T+ +A Y +HWR
Sbjct: 67 IISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWR 126
Query: 133 KLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKPLVKELTMNITM 192
LRR+ LEI + RLN+ IRRDE+RR + ++ S GF KVE K + +LT N +
Sbjct: 127 NLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNNII 186
Query: 193 RMVAGKRYFGEEAAKSSEARTFQAIVDELFEFTLSSYPADFLPILKYIDIQGFMKRAKKL 252
RM+AGK Y+G+ EA+ + ++ E + AD++PIL +I R KKL
Sbjct: 187 RMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS--ETRIKKL 246
Query: 253 ITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIKGLILTMILGG 312
R+D F QGL+DE R G+ E+ +N MV H L LQETQPEYY D IIKG +L++I GG
Sbjct: 247 AGRLDEFLQGLVDEKREGK---EKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGG 306
Query: 313 SDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSYLRCIVLETLRL 372
+DTTAVT+EWA+S+LLN+P VL KA+ EI+ +G D+LLEE+D+ L YL+ IV ETLRL
Sbjct: 307 TDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRL 366
Query: 373 YPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRPERFENNNGE 432
YPA P+LLPH +S +C + GYD+PR T+LL NAWAIHRDP LW+DPTSF+PERFE
Sbjct: 367 YPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEK---- 426
Query: 433 GDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVDMNEGKGVSMP 492
+G K ++ FGLGRRACPG+G+A R++ L LG LIQCF+W+ I +EVDM EG G++MP
Sbjct: 427 -EGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMP 486
Query: 493 KAQPLEALCKAREIATKLL 509
KA+PLEA+C+AR+ K+L
Sbjct: 487 KARPLEAMCRARDFVGKIL 495
BLAST of PI0002984 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 501.5 bits (1290), Expect = 7.9e-142
Identity = 256/509 (50.29%), Postives = 349/509 (68.57%), Query Frame = 0
Query: 3 DSILQYSAFSILSIAVVVLAIIKYWLNFNSNKNLPPSPP-TIPIIGHLHLLKLPVHRTLQ 62
+ IL S S+ ++A+ +L K L NLPPSP ++P+IGHLHLLK P+HRT
Sbjct: 4 NQILILSFLSLFTLAIFLLTRSKRKL------NLPPSPAISLPVIGHLHLLKPPLHRTFL 63
Query: 63 TLSQKYG--PVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTL 122
+LS+ G PV LR G+RLV V++S AEECFTKNDV+ ANRP F +S L YN T L
Sbjct: 64 SLSKSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYL 123
Query: 123 GAAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEASRDGFGKVEFKPL 182
+A YGDHWR LRR++ +EI + RLN+ IR+DE+RR + + S GF +VE K L
Sbjct: 124 LSASYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTL 183
Query: 183 VKELTMNITMRMVAGKRYFGEEAAKSSEARTFQAIVDELFEFTLSSYPADFLPILKYIDI 242
+ L N T+RM+AGKRYFGE+ + +A+ + +V E + P D+L IL++ +
Sbjct: 184 LTNLASNTTIRMLAGKRYFGED---NDDAKLVKNLVSEAVTSAGAGNPIDYLSILRW--V 243
Query: 243 QGFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPEYYTDDIIK 302
+ KR K L R D F Q L+DE R + +E M+ H L LQ+ QP+YYTD IIK
Sbjct: 244 SSYEKRIKNLGNRFDTFLQKLVDEKR----AEKEKGETMIDHLLALQDIQPDYYTDVIIK 303
Query: 303 GLILTMILGGSDTTAVTIEWAMSNLLNHPSVLEKAKAEIESQLGGDKLLEETDLSKLSYL 362
G+ILT+I+ G+DT++VT+EWAMSNLLNHP +L+KA+ EI+ ++G D+L++E+D+ LSYL
Sbjct: 304 GIILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYL 363
Query: 363 RCIVLETLRLYPAGPLLLPHKSSANCSISGYDVPRDTILLVNAWAIHRDPTLWEDPTSFR 422
+ IVLETLR+YPA PLLLPH SS +C + GYD+P T++L NAWA+HRDP +WEDP F+
Sbjct: 364 QSIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFK 423
Query: 423 PERFENNNGEGDGNNKLMIAFGLGRRACPGTGMAHRVMGLMLGMLIQCFDWKSIDGKEVD 482
PERFE +G + +I+FG+GRRACPG G+AHR++ LG L+QCF+W+ + VD
Sbjct: 424 PERFEK-----EGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVD 483
Query: 483 MNEGKGVSMPKAQPLEALCKAREIATKLL 509
M E KG ++PKA PL A+CKAR I KL+
Sbjct: 484 MTEDKGATLPKAIPLRAMCKARSIVDKLI 492
BLAST of PI0002984 vs. TAIR 10
Match:
AT3G28740.1 (Cytochrome P450 superfamily protein )
HSP 1 Score: 499.6 bits (1285), Expect = 3.0e-141
Identity = 256/478 (53.56%), Postives = 331/478 (69.25%), Query Frame = 0
Query: 35 NLPPSPP-TIPIIGHLHLLKLPVHRTLQTLSQKY--GPVLSLRFGSRLVVVVTSLEAAEE 94
NLPPSP PIIGHLHLLKLP+HR +LS+ + SL GSRLV VV+S AEE
Sbjct: 38 NLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSESLNNAKIFSLSLGSRLVFVVSSHAVAEE 97
Query: 95 CFTKNDVIFANRPNFAVSHCLSYNETTLGAAPYGDHWRKLRRVSTLEILCSSRLNANYEI 154
CFTKNDV+ ANRP F V + YN TT+ A YGD WR LRR+ T+EI S RLN+ I
Sbjct: 98 CFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGDSWRNLRRIGTIEIFSSLRLNSFVSI 157
Query: 155 RRDEVRRAMKKIYEASRDGFGKVEFKPLVKELTMNITMRMVAGKRYFGEEAAKSSEARTF 214
R+DE+RR + + + S+ GF KVE KPL LT+N +RMVAGKR++G+ +EA+
Sbjct: 158 RQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLTINNIIRMVAGKRFYGDGTENDNEAKHV 217
Query: 215 QAIVDELFEFTLSSYPADFLPILKYIDIQGFMKRAKKLITRVDAFWQGLIDEHRRGEISN 274
+ ++ E+ + AD+ PIL+Y + + K KKL RVD F Q L++E R ++
Sbjct: 218 RQLIAEVVVSGGAGNAADYFPILRY--VTNYEKHVKKLAGRVDEFLQSLVNEKRVEKVKG 277
Query: 275 EEMKNCMVAHFLKLQETQPEYYTDDIIKGLILTMILGGSDTTAVTIEWAMSNLLNHPSVL 334
N M+ H L LQETQP+YYTD IIKG+IL MIL G+DT+A T+EWAMSNLLNHP VL
Sbjct: 278 ----NTMIDHLLSLQETQPDYYTDVIIKGIILVMILAGTDTSAGTLEWAMSNLLNHPEVL 337
Query: 335 EKAKAEIESQLGGDKLLEETDLSKLSYLRCIVLETLRLYPAGPLLLPHKSSANCSISGYD 394
KAK EI+ Q+G D+L+EE D+ KL YL+ IV ETLRLYP P+LLPH +S +C + GYD
Sbjct: 338 RKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLRLYPVAPMLLPHLASEDCIVDGYD 397
Query: 395 VPRDTILLVNAWAIHRDPTLWEDPTSFRPERFENNNGEGDGNNKLMIAFGLGRRACPGTG 454
VPR TI+LVNAWAIHRDP LWE+P F+PERFE G +K ++ FG+GRR+CPG+G
Sbjct: 398 VPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEKK-----GEDKKLMPFGIGRRSCPGSG 457
Query: 455 MAHRVMGLMLGMLIQCFDWKSIDGKEVDMNEG-KGVSMPKAQPLEALCKAREIATKLL 509
+A R++ L LG L+QCF+W+ ++ K +DM E KG +M KA L+A+CKAR I K+L
Sbjct: 458 LAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKATSLQAMCKARPIVHKVL 504
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
W8JMU7 | 1.5e-150 | 52.55 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
Q6WNQ8 | 2.9e-149 | 52.34 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
Q6WNQ9 | 5.5e-148 | 52.30 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
P93147 | 1.8e-143 | 49.02 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
Q6WNR0 | 3.8e-141 | 48.12 | Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A5D3DLU7 | 2.1e-283 | 96.26 | Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... | [more] |
A0A1S3CAW8 | 2.1e-283 | 96.26 | isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103498910 PE=3 SV=1 | [more] |
A0A097IYP1 | 1.5e-281 | 95.28 | Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa6G088160 PE=2 SV=1 | [more] |
A0A6J1H614 | 3.4e-233 | 79.92 | isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111460794 PE=... | [more] |
A0A6J1KS03 | 1.8e-231 | 78.94 | isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111498126 PE=3 ... | [more] |
Match Name | E-value | Identity | Description | |
XP_008459942.1 | 4.3e-283 | 96.26 | PREDICTED: isoflavone 3'-hydroxylase-like [Cucumis melo] >KAA0039878.1 isoflavon... | [more] |
NP_001289568.1 | 3.1e-281 | 95.28 | isoflavone 2'-hydroxylase-like [Cucumis sativus] >AIT72031.1 cytochrome P450 [Cu... | [more] |
KGN46394.2 | 3.1e-281 | 95.28 | hypothetical protein Csa_005702 [Cucumis sativus] | [more] |
XP_038907094.1 | 1.9e-259 | 88.78 | cytochrome P450 81Q32-like [Benincasa hispida] | [more] |
KAG7025244.1 | 1.4e-233 | 79.92 | Isoflavone 2'-hydroxylase [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
Match Name | E-value | Identity | Description | |
AT4G37310.1 | 5.1e-149 | 51.69 | cytochrome P450, family 81, subfamily H, polypeptide 1 | [more] |
AT4G37370.1 | 2.9e-144 | 51.20 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
AT4G37360.1 | 2.7e-142 | 51.50 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |
AT4G37330.1 | 7.9e-142 | 50.29 | cytochrome P450, family 81, subfamily D, polypeptide 4 | [more] |
AT3G28740.1 | 3.0e-141 | 53.56 | Cytochrome P450 superfamily protein | [more] |