PI0002462 (gene) Melon (PI 482460) v1

Overview
NamePI0002462
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionTVP38/TMEM64 family membrane protein slr0305-like
Locationchr11: 4765171 .. 4769370 (+)
RNA-Seq ExpressionPI0002462
SyntenyPI0002462
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTCACATGGCGCTCCGCCCTCAAGATTACCCTCTTACTCCTACTTCTGGCTGCTGCAGTAACTGCCTGTTTTACCCTCCCAATCGAGAAGGTATCAATTTCTTGTTTCTTCCTCATCACCTACTTGAATTGTCGCATTTCCATTTTCATCTGTTCTTCAATTTCTGAGGTCTGGATTATCTCATCAAATTAATGCTCTCAAGGAATTTCTTGATTTACGTGTTTGTGTGAATGTTTCAATGTTATACCGTGTTTGACGAATTTTTCGTGTCTAAATGTAATTTTGATGTTATATGATTTGTATCGATCATATATTTGAAAATCCAACACCGTTGCAGATTCTGAAGGACTTTTTACTATGGGTTCATGAAGATCTTGGCGCTTGGGGTCCACTTGTGCTGTAAGTCGGTTAGCTTAGTCCCTTGTAGAACATTTTAAGTTGGTTTTAAATTATGTCTGTATTATCGCTTTCGCTTTCTCTCACTACTGGGGATATATTGTTTTAGGTCTATTGCATACATACCTTTGACAGTGCTGGCAGTTCCTGCCTCGATACTTACTGTAAGCATCAAATTTAATGCTTCTTATACCTGGAAAAGATATAATGTGTAGTGATGAAGGTGACTGGAATTGACTTATTTGTCTGTCTTTCAGCTTGGTGGTGGTTATCTATTTGGGTTACCTGTGGGCTTTGTTGCTGATTCCATTGGTGCAACTGTTGGGGCTGGGGCAGCTTTCCTTCTTGGGCGAACTGTACTGTTTGAACCCTTATACTTTGTCTGATATTCATATTAAGTCTGCGAAGCTTTGTATTTGATTATAGAATTATTATTGGCCTAGTTCGTTCTTTCTTTTGTATTTTATAATTGTCAAGTCATTATTAGATCAAATATGTATGATTCCCAGTTTGTCTGATAATCCTTTTATGACATTTCTTACAGATTGGAAAGTCATTTGTTGTTTCCAAGCTGAAGGATTATCCACAGTTTCATTCGGTTGCAATTGCTATTAATAAGTCTGGATTTAAGGTTTGTCCATTTTTGCGTACTTCACCTTGTTTGTTTACAGGTGTTTGCATATTATATATAGGAAAGGAAGACTGTCATCTGCGAATTCATATGCATCTTTCCTTATCTGTTATTCAATCTTTGAAATCACTTGTTTCTTCTGTTCCAATGGAAGAGAAATAACACGTCGTATGTGTGGATTTCAGTTCATTTTCTTCTTTATTTAATGTATATGAATGGCATTCGATATTTCATGAATACCAATTCTTGCTTATGTCATATGTGTAGATCATCTACGATCTATCTATGAAAATGTCTCTCCTTACAATTGCATGATGGGAAGCATATTGTGCTGTAAATGAAGTATAAATTAAATTTTGAACTGCAGTCCTAGAGTTGAGAGAGGATGCCAATGCACTTAGTTTGCTAAACTGAAATATGTGATTTCTATAAATTGTGAGTAGGCTACTGGGTGTTTGCCAATGAGTGGAAATTAATGTAGAATAATATTTGGCTATTTAGACTGTTCTCTTGGTGCTTGGGAGAAAAGTTAATTTTGTCTTAGTACCTATGAAAATGCTGACTGATAAGAAATAGATTCTTATTTTGCTTTCATTAGATTGGCATCTCTGGTATTTGTATTTGAAATGACTGTGCATGGTACCTGTTCACACTTCACCCACTGTATATATCTCACTTGGAATTTGGAAATTATCAGGAATAAGTTACGGATACTTTTAGTGACCCAAAGTTTTCACTTTTGCAGATCGTTTTGCTGCTTCGGCTTGTACCTTTACTTCCATTCAACATGTTGAATTACCTACTGTCCGTGACTCCTGTTTCACTAGGGGAATACATGTTGGCTTCATGGTTGGGAATGATGGTACTTTCTTTCTCATATTCTTATAACATTAGTTCTGACCTTATGTATTTCAGATCACAAATTTGGCATAATTTGTATGTTGATCAGAGTGTTTTATCTAGCCAAGCATAAACTGTTGGTTATTTCTGTTTCATTTGTATGATCTTGTCTTGTTGCAAAATTTATTAAATTTGTCTCTCCAAATTTTAGTTGTCTGTCCATTTGTAGTGCATGGATAGGCATATTTTTATGTTGCAAAGAGTGATAACTTTTAACTGCATTTATTTTTCTGTCACGACTAGCTAAATCAATATTGTAGTTTTCAGCCCTTTGGGCTCTTGTTAGTTCATGATGGTAACTTCATTGATTACGAAGGAAAATATCGTATAGGATTCCGGGGATAGTTTAAGAGGGCTGAAGAGGAAGTCTGTTAGGAAGAAAAAAAGGCAATGTGCCATCTGCCAAGTATAATTCAGATAGCCTTTTGAGAGGACTAGCTCAGTCTTAGTCTTAGCATATCTCACACTGGCCACAATTTTGGTATAAATAATCCTCCTAGTCATTTCTAAATGTTATTCCCATTCCCTAGGCCTGTTTGTCCTTCTAGATAGATAGGCCTGGCTCGTCCAACATCCATATTCTGTGTTTTTAGTCTTCTTGGATAAACATTTAATATTAAGGAGGGCCTAGCATAATGGTTACCAATGTGAAGTTATTGTTGAATGCTACAATATCTAGGTAGACATTGTTCTTCATCCAGGTGAAAGATGGTAATTTTACAAGTTAGCTATTCTTTGTTCGCTTCTGTTCTGCGTGGGGCATGTTTCAGAATCACCATGATAGAACCAAGGAATTATGTCTTTTCTTTTAAAATATCTACATCTATCAACCTTAGGAGCTAATTTTAATTACAAATTTTGATCTTTCACCATCTCTGTTGCAGCCCATCACACTTGCATTAGTATATGTTGGAACCACACTCAAAGATCTGTCGGATGTTACACATGGTTGGGGAGAATTTTCAAAGACTCGTTGGGTAAGTACTCTTTAACTTCTACTGTATGTGTATATATAAAACAACTATTTTATCGAGGGGGAAAAGAAAGAATACCCCATACAAATATCTAAGCCCACAAAAACCCTCTATAGAAAGGAGTTCTAAGTAAAATGTTGTCTAAAGAGTAATTACAAAAGTTTTCAGAAGTTGAAATCTAAAGAGAAATATGAAATCTCATCCAGGAACAAATATCACAAGGGTTCTTCTCCACATCTCTAAAAATTTTGTTATTTATCCGGTCCCAATATCCCATAACAAAGCGTACACCCCAACGTACCATAAAAAATGCCCCTTTTCTACTAAAAAGCGGATAAGAGGAGGATCTCCCTGATTGAAATGTTCTTTATCTTCTACTTTGGGAAGGGAGAGCAACATCTCTTTTATGGCTGTTGAGTATCAAGATCTTGATGGCTTCACCATTAACGTGGAGATTTTGCTCCCCTATTTAATTTGTTTATGAAATTGTTTCTTCATTTAAAAAAAAAAAAAAAAGAAGAAAAAAAAGTCAAGATCCTGATGGCATGTTTCTTGCTCGGACAGTCAAAATGTAGCACACTTATATCATGAAATATGAGAGTTCGTATATCAAACATATGCTATAGAAGTGGCACTGGTTTTTTGAAGAGCTTCCGAATTTTACACTATATGATATATCCTTTATTCTTTCTTGTTTGACGCAATTTCTTTCCTTTATTGTGGATGCAGGCATTCATAATATTGGGCCTTGTGGTATCTGGTAAGTTAATATTATCTGCCGAAACTTATATCCTTTTTCTGTTTCCGGCAAGCAGCTTTTTCTACAAAGATCAAAAGTGGACAATAATTCTAAGAAAAACGTGTGAGACATTAAAAAAAAAGGAAAATGTCCTTTTAGTCTTATCAAAATAAAGATTTGTGTCTTGTATGCAACATCTGCTGTGCCAGGAAAAATCTTTGAGCATCTTTGTCAATTAAAATTACATTAGATGGTTCAATAATTAAAATAAATAAAAAAATTGTTATTTAGCTTTCATTTCAAATAAAGTTTTAGAAAAAGAGGGAACCGTACCGGGAGGCTTTTCTGACATTGATTTACATGCAGTGGTTCTGGCGTACTGTGTTACCAGAGTTGCAAAGGCTGCTTTGGAAAAAGCTTTGGCTGAGAACGGAGATGGTGTTGATTATTTGGGTCTCTCCTCGCAGCTACCCATTGTAGATGAATCGCCTGTTGATCTTAACCAGCCTCTCATAATCAAGATCGACTCTCCTCAAGATGATGATCGTAAATGA

mRNA sequence

ATGGCTTTCACATGGCGCTCCGCCCTCAAGATTACCCTCTTACTCCTACTTCTGGCTGCTGCAGTAACTGCCTGTTTTACCCTCCCAATCGAGAAGATTCTGAAGGACTTTTTACTATGGGTTCATGAAGATCTTGGCGCTTGGGGTCCACTTGTGCTGTCTATTGCATACATACCTTTGACAGTGCTGGCAGTTCCTGCCTCGATACTTACTCTTGGTGGTGGTTATCTATTTGGGTTACCTGTGGGCTTTGTTGCTGATTCCATTGGTGCAACTGTTGGGGCTGGGGCAGCTTTCCTTCTTGGGCGAACTATTGGAAAGTCATTTGTTGTTTCCAAGCTGAAGGATTATCCACAGTTTCATTCGGTTGCAATTGCTATTAATAAGTCTGGATTTAAGATCGTTTTGCTGCTTCGGCTTGTACCTTTACTTCCATTCAACATGTTGAATTACCTACTGTCCGTGACTCCTGTTTCACTAGGGGAATACATGTTGGCTTCATGGTTGGGAATGATGCCCATCACACTTGCATTAGTATATGTTGGAACCACACTCAAAGATCTGTCGGATGTTACACATGGTTGGGGAGAATTTTCAAAGACTCGTTGGGCATTCATAATATTGGGCCTTGTGGTATCTGTGGTTCTGGCGTACTGTGTTACCAGAGTTGCAAAGGCTGCTTTGGAAAAAGCTTTGGCTGAGAACGGAGATGGTGTTGATTATTTGGGTCTCTCCTCGCAGCTACCCATTGTAGATGAATCGCCTGTTGATCTTAACCAGCCTCTCATAATCAAGATCGACTCTCCTCAAGATGATGATCGTAAATGA

Coding sequence (CDS)

ATGGCTTTCACATGGCGCTCCGCCCTCAAGATTACCCTCTTACTCCTACTTCTGGCTGCTGCAGTAACTGCCTGTTTTACCCTCCCAATCGAGAAGATTCTGAAGGACTTTTTACTATGGGTTCATGAAGATCTTGGCGCTTGGGGTCCACTTGTGCTGTCTATTGCATACATACCTTTGACAGTGCTGGCAGTTCCTGCCTCGATACTTACTCTTGGTGGTGGTTATCTATTTGGGTTACCTGTGGGCTTTGTTGCTGATTCCATTGGTGCAACTGTTGGGGCTGGGGCAGCTTTCCTTCTTGGGCGAACTATTGGAAAGTCATTTGTTGTTTCCAAGCTGAAGGATTATCCACAGTTTCATTCGGTTGCAATTGCTATTAATAAGTCTGGATTTAAGATCGTTTTGCTGCTTCGGCTTGTACCTTTACTTCCATTCAACATGTTGAATTACCTACTGTCCGTGACTCCTGTTTCACTAGGGGAATACATGTTGGCTTCATGGTTGGGAATGATGCCCATCACACTTGCATTAGTATATGTTGGAACCACACTCAAAGATCTGTCGGATGTTACACATGGTTGGGGAGAATTTTCAAAGACTCGTTGGGCATTCATAATATTGGGCCTTGTGGTATCTGTGGTTCTGGCGTACTGTGTTACCAGAGTTGCAAAGGCTGCTTTGGAAAAAGCTTTGGCTGAGAACGGAGATGGTGTTGATTATTTGGGTCTCTCCTCGCAGCTACCCATTGTAGATGAATCGCCTGTTGATCTTAACCAGCCTCTCATAATCAAGATCGACTCTCCTCAAGATGATGATCGTAAATGA

Protein sequence

MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPLTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINKSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVYVGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVDYLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK
Homology
BLAST of PI0002462 vs. ExPASy Swiss-Prot
Match: Q55909 (TVP38/TMEM64 family membrane protein slr0305 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr0305 PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 2.5e-30
Identity = 77/200 (38.50%), Postives = 123/200 (61.50%), Query Frame = 0

Query: 34  LKDFLLWVHEDLGAWGPLVLSIAYIPLTVLAVPASILTLGGGYLFGLPVGFVADSIGATV 93
           L + L W+ + LG W  +   + Y   TV+ +P SILTLG G +FG+ +G +   IGAT+
Sbjct: 5   LLNALQWI-DGLGTWAAIAFMLLYTVATVVFLPGSILTLGAGVVFGVILGSIYVFIGATL 64

Query: 94  GAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINKSGFKIVLLLRLVPLLPFNMLNYLL 153
           GA AAFL+GR + + +V  K+    +F ++  A+ K G KIV+L RL P+ PFN+LNY  
Sbjct: 65  GATAAFLVGRYLARGWVAKKIAGNQKFKAIDEAVGKEGLKIVILTRLSPVFPFNLLNYAY 124

Query: 154 SVTPVSLGEYMLASWLGMMPITLALVYVGTTLKDLSDVTHGWGEFSKT-RWAFIILGLVV 213
            +T VSL +Y++ S LGM+P T+  VY+G+    L+ +     + + T +W   I+G + 
Sbjct: 125 GITNVSLKDYVIGS-LGMIPGTIMYVYIGSLAGSLATLGTATNQANPTLQWTIRIVGFIA 184

Query: 214 SVVLAYCVTRVAKAALEKAL 233
           +V +   VT++A+ AL +A+
Sbjct: 185 TVAVTIYVTKIARKALNEAI 202

BLAST of PI0002462 vs. ExPASy Swiss-Prot
Match: Q6YI46 (Transmembrane protein 64 OS=Homo sapiens OX=9606 GN=TMEM64 PE=2 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 2.8e-13
Identity = 62/226 (27.43%), Postives = 116/226 (51.33%), Query Frame = 0

Query: 12  TLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPLTVLAVPAS--- 71
           +L+L+ + AA+       + + L   LLWV       G L+  + +I   V++ P     
Sbjct: 122 SLVLVCVLAALCFASLALVRRYLHHLLLWVESLDSLLGVLLFVVGFI---VVSFPCGWGY 181

Query: 72  -ILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSV-AIA 131
            +L +  GYL+G  +G     +G  +G   A ++ + +  ++V ++++   +  +V  + 
Sbjct: 182 IVLNVAAGYLYGFVLGMGLMMVGVLIGTFIAHVVCKRLLTAWVAARIQSSEKLSAVIRVV 241

Query: 132 INKSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVYVGTTLK 191
              SG K+V L RL P +PF + N + S+T +SL  Y++AS +G++P  L   Y+GTTL+
Sbjct: 242 EGGSGLKVVALARLTP-IPFGLQNAVFSITDLSLPNYLMASSVGLLPTQLLNSYLGTTLR 301

Query: 192 DLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKAL 233
            + DV     E S + +    L +++S+ L + V   A+  L  A+
Sbjct: 302 TMEDVI---AEQSVSGYFVFCLQIIISIGLMFYVVHRAQVELNAAI 340

BLAST of PI0002462 vs. ExPASy Swiss-Prot
Match: Q3U145 (Transmembrane protein 64 OS=Mus musculus OX=10090 GN=Tmem64 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.6e-13
Identity = 63/226 (27.88%), Postives = 116/226 (51.33%), Query Frame = 0

Query: 12  TLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPLTVLAVPAS--- 71
           +L+L+ + AA+       + + L+  LLWV       G L+  + +I   V++ P     
Sbjct: 123 SLVLVCVLAALCFASLALVRRYLQHLLLWVESLDSLLGVLLFVVGFI---VVSFPCGWGY 182

Query: 72  -ILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSV-AIA 131
            +L +  GYL+G  +G     +G  +G   A ++ + +  ++V +++++  +  +V  + 
Sbjct: 183 IVLNVAAGYLYGFVLGMGLMVVGVLIGTFIAHVVCKRLLTAWVAARIQNSDKLSAVIRVV 242

Query: 132 INKSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVYVGTTLK 191
              SG K+V L RL P +PF + N + S+T V L  Y++AS  G++P  L   Y+GTTL+
Sbjct: 243 EGGSGLKVVALARLTP-IPFGLQNAVFSITDVPLPSYLMASSAGLLPTQLLNSYLGTTLR 302

Query: 192 DLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKAL 233
            + DV     E S + +    L +V+S+ L + V   A+  L  A+
Sbjct: 303 TMEDVI---AEQSLSGYFVFCLQIVISIGLMFYVVHRAQVELNAAI 341

BLAST of PI0002462 vs. ExPASy Swiss-Prot
Match: P76219 (TVP38/TMEM64 family membrane protein YdjX OS=Escherichia coli (strain K12) OX=83333 GN=ydjX PE=3 SV=2)

HSP 1 Score: 68.2 bits (165), Expect = 1.7e-10
Identity = 47/166 (28.31%), Postives = 83/166 (50.00%), Query Frame = 0

Query: 10  KITLLLLLLAAAVTACFTLPIEKILKDF--LLWVHEDLGAWGPLVLSIAYIPLTVLAVPA 69
           K     L+ A  + A     +  +L D   L  +    G +G  +  + +I  T+L +P 
Sbjct: 6   KFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSGFFGYSLYILLFIIATLLLLPG 65

Query: 70  SILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAI 129
           SIL + GG +FG  +G +   I AT+ +  +FLL R +G+  ++  +     F ++   I
Sbjct: 66  SILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARWLGRDLLLKYVGHSNTFQAIEKGI 125

Query: 130 NKSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMP 174
            ++G   ++L RL+PL P+N+ NY   +T ++   Y L S L  +P
Sbjct: 126 ARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWPYTLISALTTLP 171

BLAST of PI0002462 vs. ExPASy Swiss-Prot
Match: P9WFS2 (TVP38/TMEM64 family membrane protein MT1538 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT1538 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 2.1e-08
Identity = 50/171 (29.24%), Postives = 84/171 (49.12%), Query Frame = 0

Query: 12  TLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPLTVLAVPASILT 71
           T++ + +   V     +P    L+D   W  + LGAW PL   + +  +TV   P +  T
Sbjct: 35  TVVGITVLVVVALLVPVPTAVELRD---WA-KSLGAWFPLAFLLVHTVVTVPPFPRTAFT 94

Query: 72  LGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINKSG 131
           L  G LFG  VG     +G+T  A  A LL R  G  + ++ L      + +   + + G
Sbjct: 95  LAAGLLFGSVVGVFIAVVGSTASAVIAMLLVRATG--WQLNSLVRRRAINRLDERLRERG 154

Query: 132 FKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVYVG 183
           +  +L LRL+P++PF  +NY    + V +  +  A+  G++P T A+V +G
Sbjct: 155 WLAILSLRLIPVVPFAAINYAAGASGVRILSFAWATLAGLLPGTAAVVILG 199

BLAST of PI0002462 vs. ExPASy TrEMBL
Match: A0A0A0KC10 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G237630 PE=4 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 1.3e-143
Identity = 273/275 (99.27%), Postives = 274/275 (99.64%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL
Sbjct: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINKSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180
           HSVAIAIN+SGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITL LVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240

Query: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK 276
           YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK
Sbjct: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK 275

BLAST of PI0002462 vs. ExPASy TrEMBL
Match: A0A1S3C8Q0 (TVP38/TMEM64 family membrane protein slr0305-like OS=Cucumis melo OX=3656 GN=LOC103497924 PE=4 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 5.1e-143
Identity = 273/275 (99.27%), Postives = 274/275 (99.64%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL
Sbjct: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINKSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180
           HSVAIAIN+SGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240

Query: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK 276
           YLGLSSQLPIVDESPVDLNQPLIIKIDSPQ DDRK
Sbjct: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQVDDRK 275

BLAST of PI0002462 vs. ExPASy TrEMBL
Match: A0A6J1KD88 (uncharacterized protein LOC111494568 OS=Cucurbita maxima OX=3661 GN=LOC111494568 PE=4 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 1.5e-134
Identity = 259/275 (94.18%), Postives = 265/275 (96.36%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKIT  LLLLAA VTACFTLPIEKILKDFLLWV +DLGAWGPLVLS+AYIPL
Sbjct: 1   MAFTWRSALKITAALLLLAAVVTACFTLPIEKILKDFLLWVDQDLGAWGPLVLSVAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINKSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180
           HSVAIAIN+SGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPIT ALVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGEFSKTRWAFI+LGLVVSVVLAYCVTRVAKAALEKALAENGD VD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIMLGLVVSVVLAYCVTRVAKAALEKALAENGDAVD 240

Query: 241 YLGLSSQLPIVDES-PVDLNQPLIIKIDSPQDDDR 275
           YLG+SSQLPIV ES PVDLNQPLIIKIDSP DD +
Sbjct: 241 YLGISSQLPIVAESAPVDLNQPLIIKIDSPPDDSK 275

BLAST of PI0002462 vs. ExPASy TrEMBL
Match: A0A6J1EQJ6 (uncharacterized protein LOC111436796 OS=Cucurbita moschata OX=3662 GN=LOC111436796 PE=4 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 2.5e-134
Identity = 259/273 (94.87%), Postives = 264/273 (96.70%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKIT  LLLLAA VTACFTLPIEKILKDFLLWV +DLGAWGPLVLS+AYIPL
Sbjct: 1   MAFTWRSALKITAALLLLAAVVTACFTLPIEKILKDFLLWVDQDLGAWGPLVLSVAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINKSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180
           HSVAIAIN+SGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPIT ALVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGEFSKTRWAFI+LGLVVSVVLAYCVTRVAKAALEKALAENGD VD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIMLGLVVSVVLAYCVTRVAKAALEKALAENGDAVD 240

Query: 241 YLGLSSQLPIVDES-PVDLNQPLIIKIDSPQDD 273
           YLG+SSQLPIV ES PVDLNQPLIIKIDSP DD
Sbjct: 241 YLGISSQLPIVAESAPVDLNQPLIIKIDSPPDD 273

BLAST of PI0002462 vs. ExPASy TrEMBL
Match: A0A6J1H6Q0 (uncharacterized protein LOC111460118 OS=Cucurbita moschata OX=3662 GN=LOC111460118 PE=4 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.2e-131
Identity = 252/272 (92.65%), Postives = 261/272 (95.96%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKIT LL+L+AAAV ACFTLPIEKILKDFLLWV +DLGAWGPLVLS+AYIPL
Sbjct: 1   MAFTWRSALKITFLLILVAAAVIACFTLPIEKILKDFLLWVDQDLGAWGPLVLSLAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINKSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180
           HSVAIAIN+SGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGE SKTR AFIILGLVVS +LAYC+TRVAKAALEKALAENGD VD
Sbjct: 181 VGTTLKDLSDVTHGWGELSKTRLAFIILGLVVSALLAYCITRVAKAALEKALAENGDAVD 240

Query: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDD 273
           YLGLS +LP+  ESPVDLN PLIIKIDSPQDD
Sbjct: 241 YLGLSPRLPVAAESPVDLNLPLIIKIDSPQDD 272

BLAST of PI0002462 vs. NCBI nr
Match: XP_004150898.1 (uncharacterized protein LOC101204495 [Cucumis sativus] >XP_011657201.1 uncharacterized protein LOC101204495 [Cucumis sativus] >KGN47260.1 hypothetical protein Csa_017041 [Cucumis sativus])

HSP 1 Score: 518.8 bits (1335), Expect = 2.8e-143
Identity = 273/275 (99.27%), Postives = 274/275 (99.64%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL
Sbjct: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINKSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180
           HSVAIAIN+SGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITL LVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240

Query: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK 276
           YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK
Sbjct: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK 275

BLAST of PI0002462 vs. NCBI nr
Match: XP_008458555.1 (PREDICTED: TVP38/TMEM64 family membrane protein slr0305-like [Cucumis melo] >XP_008458556.1 PREDICTED: TVP38/TMEM64 family membrane protein slr0305-like [Cucumis melo])

HSP 1 Score: 516.9 bits (1330), Expect = 1.1e-142
Identity = 273/275 (99.27%), Postives = 274/275 (99.64%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL
Sbjct: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINKSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180
           HSVAIAIN+SGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240

Query: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK 276
           YLGLSSQLPIVDESPVDLNQPLIIKIDSPQ DDRK
Sbjct: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQVDDRK 275

BLAST of PI0002462 vs. NCBI nr
Match: XP_038906539.1 (TVP38/TMEM64 family membrane protein slr0305-like [Benincasa hispida] >XP_038906541.1 TVP38/TMEM64 family membrane protein slr0305-like [Benincasa hispida] >XP_038906542.1 TVP38/TMEM64 family membrane protein slr0305-like [Benincasa hispida] >XP_038906543.1 TVP38/TMEM64 family membrane protein slr0305-like [Benincasa hispida] >XP_038906544.1 TVP38/TMEM64 family membrane protein slr0305-like [Benincasa hispida] >XP_038906545.1 TVP38/TMEM64 family membrane protein slr0305-like [Benincasa hispida])

HSP 1 Score: 493.0 bits (1268), Expect = 1.6e-135
Identity = 264/278 (94.96%), Postives = 268/278 (96.40%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKIT+ LLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLS+AYIPL
Sbjct: 1   MAFTWRSALKITVFLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSVAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVL VPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLVVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINKSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180
           HSVAIAIN+SGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDG-V 240
           VGTTLKDLSDVTHGWGE SKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAE GDG V
Sbjct: 181 VGTTLKDLSDVTHGWGELSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAETGDGAV 240

Query: 241 DYLGLSSQLPIV--DESPVDLNQPLIIKIDSPQDDDRK 276
           DYLGLSSQLPIV  +   VDLNQPLIIKIDSPQDDDRK
Sbjct: 241 DYLGLSSQLPIVAAESHAVDLNQPLIIKIDSPQDDDRK 278

BLAST of PI0002462 vs. NCBI nr
Match: XP_023000292.1 (uncharacterized protein LOC111494568 [Cucurbita maxima])

HSP 1 Score: 488.8 bits (1257), Expect = 3.1e-134
Identity = 259/275 (94.18%), Postives = 265/275 (96.36%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKIT  LLLLAA VTACFTLPIEKILKDFLLWV +DLGAWGPLVLS+AYIPL
Sbjct: 1   MAFTWRSALKITAALLLLAAVVTACFTLPIEKILKDFLLWVDQDLGAWGPLVLSVAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINKSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180
           HSVAIAIN+SGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPIT ALVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGEFSKTRWAFI+LGLVVSVVLAYCVTRVAKAALEKALAENGD VD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIMLGLVVSVVLAYCVTRVAKAALEKALAENGDAVD 240

Query: 241 YLGLSSQLPIVDES-PVDLNQPLIIKIDSPQDDDR 275
           YLG+SSQLPIV ES PVDLNQPLIIKIDSP DD +
Sbjct: 241 YLGISSQLPIVAESAPVDLNQPLIIKIDSPPDDSK 275

BLAST of PI0002462 vs. NCBI nr
Match: KAG6593916.1 (hypothetical protein SDJN03_13392, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 488.0 bits (1255), Expect = 5.2e-134
Identity = 259/273 (94.87%), Postives = 264/273 (96.70%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKIT  LLLLAA VTACFTLPIEKILKDFLLWV +DLGAWGPLVLS+AYIPL
Sbjct: 101 MAFTWRSALKITAALLLLAAVVTACFTLPIEKILKDFLLWVDQDLGAWGPLVLSVAYIPL 160

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 161 TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 220

Query: 121 HSVAIAINKSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180
           HSVAIAIN+SGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPIT ALVY
Sbjct: 221 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 280

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGEFSKTRWAFI+LGLVVSVVLAYCVTRVAKAALEKALAENGD VD
Sbjct: 281 VGTTLKDLSDVTHGWGEFSKTRWAFIMLGLVVSVVLAYCVTRVAKAALEKALAENGDAVD 340

Query: 241 YLGLSSQLPIVDES-PVDLNQPLIIKIDSPQDD 273
           YLG+SSQLPIV ES PVDLNQPLIIKIDSP DD
Sbjct: 341 YLGISSQLPIVAESAPVDLNQPLIIKIDSPPDD 373

BLAST of PI0002462 vs. TAIR 10
Match: AT5G19070.1 (SNARE associated Golgi protein family )

HSP 1 Score: 386.0 bits (990), Expect = 2.6e-107
Identity = 198/275 (72.00%), Postives = 235/275 (85.45%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTW SAL+I++LL+L+AA V AC+ LP+EK+LKDFLLWV +DLG WGP  L++AYIPL
Sbjct: 1   MAFTWGSALRISVLLILVAAIVLACYFLPVEKLLKDFLLWVEQDLGPWGPFALAVAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPAS+LTLGGGYLFGLP+GFVADS+GAT+G+GAAFLLGRTIGK FVV+KLKDYPQF
Sbjct: 61  TVLAVPASVLTLGGGYLFGLPIGFVADSVGATLGSGAAFLLGRTIGKPFVVAKLKDYPQF 120

Query: 121 HSVAIAINKSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180
            SVA+AI KSGFKI LLLRL PLLPF+MLNYLLSVTP+ LG Y+L+SWLGMMPITLALVY
Sbjct: 121 QSVALAIEKSGFKICLLLRLAPLLPFSMLNYLLSVTPIRLGPYLLSSWLGMMPITLALVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAEN-GDGV 240
           VGTTLKDLSDVTH W EFS  RWAF+I  LV+SV+L  CVT+VAK AL KALAE+ GD  
Sbjct: 181 VGTTLKDLSDVTHKWSEFSPGRWAFLISSLVISVILMVCVTKVAKDALRKALAEHGGDMN 240

Query: 241 DYLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDR 275
           + +    +L + D++  DLN+PL+IKID+ Q  D+
Sbjct: 241 EAVAALPELTVTDDASTDLNEPLLIKIDAQQPQDQ 275

BLAST of PI0002462 vs. TAIR 10
Match: AT1G03260.1 (SNARE associated Golgi protein family )

HSP 1 Score: 344.7 bits (883), Expect = 6.7e-95
Identity = 183/269 (68.03%), Postives = 224/269 (83.27%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           M+FT  S  +I + LLLL A V+A   LP+E+ LKDFLLW+ EDLG +GPL L++AYIPL
Sbjct: 4   MSFT-PSTFRIAISLLLLVAIVSAVIFLPVEQKLKDFLLWIKEDLGPFGPLALALAYIPL 63

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           T++AVPAS+LTLGGGYLFGLPVGFVADS+GAT+GA AAFLLGRTIGKS+V SK+K YP+F
Sbjct: 64  TIVAVPASVLTLGGGYLFGLPVGFVADSLGATLGATAAFLLGRTIGKSYVTSKIKHYPKF 123

Query: 121 HSVAIAINKSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180
            +V++AI KSGFKIVLLLR+VP+LPFNMLNYLLSVTPV LGEYMLA+WLGMMPIT ALVY
Sbjct: 124 QAVSVAIQKSGFKIVLLLRVVPILPFNMLNYLLSVTPVRLGEYMLATWLGMMPITFALVY 183

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSD+THGW E S  RW  +++G+ ++V+L  C+TRVAK++L+KALAENG  +D
Sbjct: 184 VGTTLKDLSDITHGWHEVSVFRWVIMMVGVALAVILIICITRVAKSSLDKALAENGTELD 243

Query: 241 --YLGLSSQLPIVDESPVDLNQPLIIKID 268
                 +S LPI  E P DL +PL+I+ID
Sbjct: 244 GKKNDDASVLPIA-EPPPDLQEPLVIRID 270

BLAST of PI0002462 vs. TAIR 10
Match: AT1G22850.1 (SNARE associated Golgi protein family )

HSP 1 Score: 93.2 bits (230), Expect = 3.5e-19
Identity = 78/234 (33.33%), Postives = 125/234 (53.42%), Query Frame = 0

Query: 7   SALKITLL--LLLLA-----AAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIP 66
           +A+K T+L  +LL+      A V   +   I   L  F  ++ E  G  G  +    Y  
Sbjct: 104 AAIKGTILAGVLLIGTVGGFAGVGYVYRDQINTFLTQFSTYI-EGYGTAGYALFIAVYAG 163

Query: 67  LTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQ 126
           L +LA+PA  LT+  G LFG  +G +  SI  T+ A  AFL+ R   +  ++  ++D  +
Sbjct: 164 LEILAIPALPLTMSAGLLFGPLIGTIIVSISGTMAASVAFLIARYFARERILKLVEDNKK 223

Query: 127 FHSVAIAINKSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALV 186
           F ++  AI ++GF++V LLRL PLLPF++ NYL  +T V    Y+L SWLGM+P + A V
Sbjct: 224 FLAIDKAIGENGFRVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGSWAYV 283

Query: 187 YVG----TTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALE 230
             G      +++ S+V    G     +   + +GL+V+ +    VT +AK A++
Sbjct: 284 SAGAFGRAIIQEESNVGLPGG---NGQLLTLGVGLLVTALAGTYVTSLAKDAIK 333

BLAST of PI0002462 vs. TAIR 10
Match: AT1G12450.1 (SNARE associated Golgi protein family )

HSP 1 Score: 55.8 bits (133), Expect = 6.2e-08
Identity = 62/202 (30.69%), Postives = 93/202 (46.04%), Query Frame = 0

Query: 30  IEKILKDFLLWVHE--DLGAWGPLVL-SIAYIPLTVLAVPASILTLGGGYLFGLPVGFVA 89
           IEK L  F+ WV     +   G L+  S+A  P  +L  P+S      G  FG   GF+ 
Sbjct: 85  IEKELIPFINWVRNTFSIPVLGLLLFASVALFPSILL--PSSPSMWMAGLTFGYGKGFLL 144

Query: 90  DSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINKS----GFKIVLLLRLVP 149
               A++G    FL+G       +   LK YP+  ++  A  +      F+ V L+R+ P
Sbjct: 145 ILSAASIGVTLPFLIGHLFLHK-MQEWLKKYPKKAAILRAAGEGTWFHQFQAVTLIRVSP 204

Query: 150 LLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVYVGTTLKDLSDVTHGWGEFSKTR 209
             P+ + NY    T V  G Y+L S +GM+P     +Y G  L+ L+         S TR
Sbjct: 205 -FPYIIYNYCALATGVHYGPYILGSLVGMVPEIFVSIYTGIMLRTLAVA-------SDTR 264

Query: 210 WAFIILGLVVSVVLAYCVTRVA 225
               ++ +VV+ VL +CVT  A
Sbjct: 265 HTLSVVEIVVN-VLGFCVTASA 274

BLAST of PI0002462 vs. TAIR 10
Match: AT4G22850.1 (SNARE associated Golgi protein family )

HSP 1 Score: 49.7 bits (117), Expect = 4.4e-06
Identity = 63/238 (26.47%), Postives = 103/238 (43.28%), Query Frame = 0

Query: 9   LKITLLLLLLAAAVTACF----TLPIEKILKDFLLWVHEDL--GAWGPLVLSIAYIPLTV 68
           +K++LL   LAA     +     L ++K L   + W          G LV +   I  T+
Sbjct: 57  VKLSLLFAFLAALAVVGYIWIGPLIMDKELIPLIQWEIRTFTHPVCGLLVFASVAIFPTI 116

Query: 69  LAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHS 128
           L +P++      G  FG   GF+     A VG    + +G+      +   L+ YP   +
Sbjct: 117 L-LPSTPSMWIAGMTFGYGYGFLLIISAAAVGVSLPYFIGQLFCHK-IQGWLERYPDQAA 176

Query: 129 VAIAINKSG----FKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLAL 188
           V  A  +      F +V L+R+ P  P+ + NY    T V  G Y+  S LGM+P     
Sbjct: 177 VLRAAGEGNWLHQFLLVTLIRISP-FPYILYNYCSVATRVKYGPYITGSLLGMVPEVFVA 236

Query: 189 VYVGTTLKDLSDVTHGWGE-FSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAEN 236
           +Y G  ++ L++ +    +  S T+    ILG + +V     +T+ AK  LE    E+
Sbjct: 237 IYTGILVRTLAEASSAEEQGLSVTQVILNILGFLATVATTILITKYAKRQLETMKKED 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q559092.5e-3038.50TVP38/TMEM64 family membrane protein slr0305 OS=Synechocystis sp. (strain PCC 68... [more]
Q6YI462.8e-1327.43Transmembrane protein 64 OS=Homo sapiens OX=9606 GN=TMEM64 PE=2 SV=2[more]
Q3U1453.6e-1327.88Transmembrane protein 64 OS=Mus musculus OX=10090 GN=Tmem64 PE=1 SV=1[more]
P762191.7e-1028.31TVP38/TMEM64 family membrane protein YdjX OS=Escherichia coli (strain K12) OX=83... [more]
P9WFS22.1e-0829.24TVP38/TMEM64 family membrane protein MT1538 OS=Mycobacterium tuberculosis (strai... [more]
Match NameE-valueIdentityDescription
A0A0A0KC101.3e-14399.27Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G237630 PE=4 SV=1[more]
A0A1S3C8Q05.1e-14399.27TVP38/TMEM64 family membrane protein slr0305-like OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A6J1KD881.5e-13494.18uncharacterized protein LOC111494568 OS=Cucurbita maxima OX=3661 GN=LOC111494568... [more]
A0A6J1EQJ62.5e-13494.87uncharacterized protein LOC111436796 OS=Cucurbita moschata OX=3662 GN=LOC1114367... [more]
A0A6J1H6Q01.2e-13192.65uncharacterized protein LOC111460118 OS=Cucurbita moschata OX=3662 GN=LOC1114601... [more]
Match NameE-valueIdentityDescription
XP_004150898.12.8e-14399.27uncharacterized protein LOC101204495 [Cucumis sativus] >XP_011657201.1 uncharact... [more]
XP_008458555.11.1e-14299.27PREDICTED: TVP38/TMEM64 family membrane protein slr0305-like [Cucumis melo] >XP_... [more]
XP_038906539.11.6e-13594.96TVP38/TMEM64 family membrane protein slr0305-like [Benincasa hispida] >XP_038906... [more]
XP_023000292.13.1e-13494.18uncharacterized protein LOC111494568 [Cucurbita maxima][more]
KAG6593916.15.2e-13494.87hypothetical protein SDJN03_13392, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT5G19070.12.6e-10772.00SNARE associated Golgi protein family [more]
AT1G03260.16.7e-9568.03SNARE associated Golgi protein family [more]
AT1G22850.13.5e-1933.33SNARE associated Golgi protein family [more]
AT1G12450.16.2e-0830.69SNARE associated Golgi protein family [more]
AT4G22850.14.4e-0626.47SNARE associated Golgi protein family [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032816SNARE associated Golgi proteinPFAMPF09335SNARE_assoccoord: 65..183
e-value: 2.1E-24
score: 86.2
NoneNo IPR availablePANTHERPTHR12677:SF51SUBFAMILY NOT NAMEDcoord: 6..267
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..25
score: 5.0
IPR015414Transmembrane protein TMEM64PANTHERPTHR12677UNCHARACTERIZEDcoord: 6..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0002462.1PI0002462.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane