PI0002285 (gene) Melon (PI 482460) v1

Overview


Name	PI0002285
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Retrovirus-related Pol polyprotein from transposon 17.6
Location	chr05: 18005026 .. 18005292 (+)
RNA-Seq Expression	PI0002285
Synteny	PI0002285

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTGACACAAGTGATGTGGCGATAGGGGCTATGCTAGGTCAAAAGAAAGATAAAGTGATCCACCCTATATACTACGCGAGCAAGACTCTCACTGAAGTTCAAGAAAATTATACTACTATAGAGAAGGAATTGCTTGCAGTGTTCTTCACAATAGAAAAGTTCAGAAGTTACATTGTTGGCTCCAAAGTTACGATACACTCTAATCATTTTGCAATCAGATACCTTATGGTGAAGAAAGATGCTAAGTCGCGGTTAATAAGATAG

mRNA sequence

ATGTGTGACACAAGTGATGTGGCGATAGGGGCTATGCTAGGTCAAAAGAAAGATAAAGTGATCCACCCTATATACTACGCGAGCAAGACTCTCACTGAAGTTCAAGAAAATTATACTACTATAGAGAAGGAATTGCTTGCAGTGTTCTTCACAATAGAAAAGTTCAGAAGTTACATTGTTGGCTCCAAAGTTACGATACACTCTAATCATTTTGCAATCAGATACCTTATGGTGAAGAAAGATGCTAAGTCGCGGTTAATAAGATAG

Coding sequence (CDS)

ATGTGTGACACAAGTGATGTGGCGATAGGGGCTATGCTAGGTCAAAAGAAAGATAAAGTGATCCACCCTATATACTACGCGAGCAAGACTCTCACTGAAGTTCAAGAAAATTATACTACTATAGAGAAGGAATTGCTTGCAGTGTTCTTCACAATAGAAAAGTTCAGAAGTTACATTGTTGGCTCCAAAGTTACGATACACTCTAATCATTTTGCAATCAGATACCTTATGGTGAAGAAAGATGCTAAGTCGCGGTTAATAAGATAG

Protein sequence

MCDTSDVAIGAMLGQKKDKVIHPIYYASKTLTEVQENYTTIEKELLAVFFTIEKFRSYIVGSKVTIHSNHFAIRYLMVKKDAKSRLIR

Homology

[Close]

BLAST of PI0002285 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 6.4e-12
Identity = 39/86 (45.35%), Postives = 54/86 (62.79%), Query Frame = 0


Query: 3   DTSDVAIGAMLGQKKDKVIHPIYYASKTLTEVQENYTTIEKELLAVFFTIEKFRSYIVGS 62
           D SDVA+GA+L Q      HP+ Y S+TL E + NY+TIEKELLA+ +  + FR Y++G 
Sbjct: 514 DASDVALGAVLSQDG----HPLSYISRTLNEHEINYSTIEKELLAIVWATKTFRHYLLGR 573

Query: 63  KVTIHSNHFAIRYLMVKKDAKSRLIR 89
              I S+H  + +L   KD  S+L R
Sbjct: 574 HFEISSDHQPLSWLYRMKDPNSKLTR 595

[Close]

BLAST of PI0002285 vs. ExPASy Swiss-Prot
Match: P20825 (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 3.0e-09
Identity = 34/86 (39.53%), Postives = 55/86 (63.95%), Query Frame = 0


Query: 3   DTSDVAIGAMLGQKKDKVIHPIYYASKTLTEVQENYTTIEKELLAVFFTIEKFRSYIVGS 62
           D S++A+GA+L Q      HPI + S+TL + + NY+ IEKELLA+ +  + FR Y++G 
Sbjct: 513 DASNLALGAVLSQNG----HPISFISRTLNDHELNYSAIEKELLAIVWATKTFRHYLLGR 572

Query: 63  KVTIHSNHFAIRYLMVKKDAKSRLIR 89
           +  I S+H  +R+L   K+  ++L R
Sbjct: 573 QFLIASDHQPLRWLHNLKEPGAKLER 594

[Close]

BLAST of PI0002285 vs. ExPASy Swiss-Prot
Match: Q8I7P9 (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.1e-08
Identity = 29/87 (33.33%), Postives = 56/87 (64.37%), Query Frame = 0


Query: 3   DTSDVAIGAMLGQKKDKVIHPIYYASKTLTEVQENYTTIEKELLAVFFTIEKFRSYIVGS 62
           D S+ AIGA+L Q       PI Y S++L + +ENY TIEKE+LA+ ++++  R+Y+ G+
Sbjct: 440 DASNWAIGAVLSQDDQGRDRPIAYISRSLNKTEENYATIEKEMLAIIWSLDNLRAYLYGA 499

Query: 63  -KVTIHSNHFAIRYLMVKKDAKSRLIR 89
             + ++++H  + + +  ++  ++L R
Sbjct: 500 GTIKVYTDHQPLTFALGNRNFNAKLKR 526

[Close]

BLAST of PI0002285 vs. ExPASy Swiss-Prot
Match: P10394 (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 4.7e-07
Identity = 31/86 (36.05%), Postives = 47/86 (54.65%), Query Frame = 0


Query: 3   DTSDVAIGAMLGQKKDKVIHPIYYASKTLTEVQENYTTIEKELLAVFFTIEKFRSYIVGS 62
           D S  A GA+L Q  +    P+ YAS+  T+ + N +T E+EL A+ + I  FR YI G 
Sbjct: 621 DASKQACGAVLTQNHNGHQLPVAYASRAFTKGESNKSTTEQELAAIHWAIIHFRPYIYGK 680

Query: 63  KVTIHSNHFAIRYLMVKKDAKSRLIR 89
             T+ ++H  + YL    +  S+L R
Sbjct: 681 HFTVKTDHRPLTYLFSMVNPSSKLTR 706

[Close]

BLAST of PI0002285 vs. ExPASy Swiss-Prot
Match: P10401 (Retrovirus-related Pol polyprotein from transposon gypsy OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 5.8e-05
Identity = 26/87 (29.89%), Postives = 52/87 (59.77%), Query Frame = 0


Query: 3   DTSDVAIGAMLGQKKDKVIHPIYYASKTLTEVQENYTTIEKELLAVFFTIEKFRSYIVGS 62
           D S   IGA+L Q+      PI   S+TL + ++NY T E+ELLA+ + + K ++++ GS
Sbjct: 500 DASASGIGAVLSQEG----RPITMISRTLKQPEQNYATNERELLAIVWALGKLQNFLYGS 559

Query: 63  K-VTIHSNHFAIRYLMVKKDAKSRLIR 89
           + + I ++H  + + +  ++  +++ R
Sbjct: 560 REINIFTDHQPLTFAVADRNTNAKIKR 582

[Close]

BLAST of PI0002285 vs. ExPASy TrEMBL
Match: A0A5A7V8R9 (RT_RNaseH domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G003510 PE=4 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.1e-29
Identity = 70/87 (80.46%), Postives = 75/87 (86.21%), Query Frame = 0


Query: 1   MCDTSDVAIGAMLGQKKDKVIHPIYYASKTLTEVQENYTTIEKELLAVFFTIEKFRSYIV 60
           MCD SDV IGAMLGQK DKVI+PIY  SKTL E QENYTT EKELLAV F IEK+ SYIV
Sbjct: 30  MCDASDVVIGAMLGQKNDKVIYPIYNMSKTLNEAQENYTTTEKELLAVVFAIEKYVSYIV 89

Query: 61  GSKVTIHSNHFAIRYLMVKKDAKSRLI 88
           GSKVTIHS+HFAIRYLMVKKDA+ +LI
Sbjct: 90  GSKVTIHSDHFAIRYLMVKKDAQPQLI 116

[Close]

BLAST of PI0002285 vs. ExPASy TrEMBL
Match: A0A5D3DS34 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G001700 PE=4 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.1e-29
Identity = 70/88 (79.55%), Postives = 74/88 (84.09%), Query Frame = 0


Query: 1   MCDTSDVAIGAMLGQKKDKVIHPIYYASKTLTEVQENYTTIEKELLAVFFTIEKFRSYIV 60
           MCD SDV +G M GQKKDKV  PIYYA KTL E +ENYTTIEKELLA+ F IEKFRSYIV
Sbjct: 78  MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 137

Query: 61  GSKVTIHSNHFAIRYLMVKKDAKSRLIR 89
           GSKVTIHS+H AIRYLM KKDAKS LIR
Sbjct: 138 GSKVTIHSDHSAIRYLMAKKDAKSWLIR 165

[Close]

BLAST of PI0002285 vs. ExPASy TrEMBL
Match: A0A5A7V586 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold154G002210 PE=4 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.1e-29
Identity = 70/88 (79.55%), Postives = 74/88 (84.09%), Query Frame = 0


Query: 1   MCDTSDVAIGAMLGQKKDKVIHPIYYASKTLTEVQENYTTIEKELLAVFFTIEKFRSYIV 60
           MCD SDV +G M GQKKDKV  PIYYA KTL E +ENYTTIEKELLA+ F IEKFRSYIV
Sbjct: 52  MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 111

Query: 61  GSKVTIHSNHFAIRYLMVKKDAKSRLIR 89
           GSKVTIHS+H AIRYLM KKDAKS LIR
Sbjct: 112 GSKVTIHSDHSAIRYLMAKKDAKSWLIR 139

[Close]

BLAST of PI0002285 vs. ExPASy TrEMBL
Match: A0A6J1DCL7 (LOW QUALITY PROTEIN: uncharacterized protein LOC111019515 OS=Momordica charantia OX=3673 GN=LOC111019515 PE=4 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 6.2e-26
Identity = 58/88 (65.91%), Postives = 73/88 (82.95%), Query Frame = 0


Query: 1   MCDTSDVAIGAMLGQKKDKVIHPIYYASKTLTEVQENYTTIEKELLAVFFTIEKFRSYIV 60
           MCD SD AIGAMLGQ+K+K++HP+YYASKTLT  Q NYTT EKELLA+ FT +KFR Y++
Sbjct: 433 MCDASDYAIGAMLGQRKNKILHPVYYASKTLTGAQLNYTTTEKELLAIVFTFDKFRXYLI 492

Query: 61  GSKVTIHSNHFAIRYLMVKKDAKSRLIR 89
           G+KV + ++H A++YL  KKDAK RLIR
Sbjct: 493 GTKVIVFTDHSALKYLFAKKDAKPRLIR 520

[Close]

BLAST of PI0002285 vs. ExPASy TrEMBL
Match: A0A087GID7 (Reverse transcriptase OS=Arabis alpina OX=50452 GN=AALP_AA7G159800 PE=4 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.1e-25
Identity = 58/88 (65.91%), Postives = 72/88 (81.82%), Query Frame = 0


Query: 1   MCDTSDVAIGAMLGQKKDKVIHPIYYASKTLTEVQENYTTIEKELLAVFFTIEKFRSYIV 60
           MCD SD A+GA+LGQKKDK +H IYYAS+TL E Q+NY T EKELLA+ F  EKFR Y++
Sbjct: 648 MCDASDFAVGAVLGQKKDKKLHAIYYASRTLDEAQQNYATTEKELLALVFAFEKFRPYLI 707

Query: 61  GSKVTIHSNHFAIRYLMVKKDAKSRLIR 89
           GSKV +H++H A++YLM KKDAK RL+R
Sbjct: 708 GSKVIVHTDHSALKYLMQKKDAKPRLLR 735

[Close]

BLAST of PI0002285 vs. NCBI nr
Match: KAA0062414.1 (Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuwa])

HSP 1 Score: 137.9 bits (346), Expect = 4.2e-29
Identity = 70/88 (79.55%), Postives = 74/88 (84.09%), Query Frame = 0


Query: 1   MCDTSDVAIGAMLGQKKDKVIHPIYYASKTLTEVQENYTTIEKELLAVFFTIEKFRSYIV 60
           MCD SDV +G M GQKKDKV  PIYYA KTL E +ENYTTIEKELLA+ F IEKFRSYIV
Sbjct: 52  MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 111

Query: 61  GSKVTIHSNHFAIRYLMVKKDAKSRLIR 89
           GSKVTIHS+H AIRYLM KKDAKS LIR
Sbjct: 112 GSKVTIHSDHSAIRYLMAKKDAKSWLIR 139

[Close]

BLAST of PI0002285 vs. NCBI nr
Match: KAA0062295.1 (hypothetical protein E6C27_scaffold154G00380 [Cucumis melo var. makuwa] >TYK26691.1 hypothetical protein E5676_scaffold313G003510 [Cucumis melo var. makuwa])

HSP 1 Score: 137.9 bits (346), Expect = 4.2e-29
Identity = 70/87 (80.46%), Postives = 75/87 (86.21%), Query Frame = 0


Query: 1   MCDTSDVAIGAMLGQKKDKVIHPIYYASKTLTEVQENYTTIEKELLAVFFTIEKFRSYIV 60
           MCD SDV IGAMLGQK DKVI+PIY  SKTL E QENYTT EKELLAV F IEK+ SYIV
Sbjct: 30  MCDASDVVIGAMLGQKNDKVIYPIYNMSKTLNEAQENYTTTEKELLAVVFAIEKYVSYIV 89

Query: 61  GSKVTIHSNHFAIRYLMVKKDAKSRLI 88
           GSKVTIHS+HFAIRYLMVKKDA+ +LI
Sbjct: 90  GSKVTIHSDHFAIRYLMVKKDAQPQLI 116

[Close]

BLAST of PI0002285 vs. NCBI nr
Match: TYK26567.1 (Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuwa])

HSP 1 Score: 137.9 bits (346), Expect = 4.2e-29
Identity = 70/88 (79.55%), Postives = 74/88 (84.09%), Query Frame = 0


Query: 1   MCDTSDVAIGAMLGQKKDKVIHPIYYASKTLTEVQENYTTIEKELLAVFFTIEKFRSYIV 60
           MCD SDV +G M GQKKDKV  PIYYA KTL E +ENYTTIEKELLA+ F IEKFRSYIV
Sbjct: 78  MCDASDVVVGDMPGQKKDKVTDPIYYAIKTLNEAEENYTTIEKELLAIVFAIEKFRSYIV 137

Query: 61  GSKVTIHSNHFAIRYLMVKKDAKSRLIR 89
           GSKVTIHS+H AIRYLM KKDAKS LIR
Sbjct: 138 GSKVTIHSDHSAIRYLMAKKDAKSWLIR 165

[Close]

BLAST of PI0002285 vs. NCBI nr
Match: WP_217833163.1 (Ty3/Gypsy family RNase HI domain-containing protein, partial [Synechococcus sp. PCC 7002])

HSP 1 Score: 131.0 bits (328), Expect = 5.2e-27
Identity = 60/88 (68.18%), Postives = 76/88 (86.36%), Query Frame = 0


Query: 1  MCDTSDVAIGAMLGQKKDKVIHPIYYASKTLTEVQENYTTIEKELLAVFFTIEKFRSYIV 60
          MCD S+  +GA+LGQ+K+K++HPIYYASKTL   QENYTT EKE+LA+ F ++KFR+Y +
Sbjct: 3  MCDASNHVVGAVLGQRKEKIMHPIYYASKTLNSFQENYTTTEKEMLAIVFAVDKFRAYFI 62

Query: 61 GSKVTIHSNHFAIRYLMVKKDAKSRLIR 89
          GSKVTI+S+H AI+YLMVKKDAK RLIR
Sbjct: 63 GSKVTIYSDHSAIKYLMVKKDAKPRLIR 90

[Close]

BLAST of PI0002285 vs. NCBI nr
Match: XP_019100932.1 (PREDICTED: uncharacterized protein LOC104789643 [Camelina sativa])

HSP 1 Score: 129.8 bits (325), Expect = 1.2e-26
Identity = 62/88 (70.45%), Postives = 72/88 (81.82%), Query Frame = 0


Query: 1    MCDTSDVAIGAMLGQKKDKVIHPIYYASKTLTEVQENYTTIEKELLAVFFTIEKFRSYIV 60
            MCD SD A+GAMLGQ+KDK +H IYYAS+TL E Q NY T EKELLAV F  EKFRSY+V
Sbjct: 973  MCDASDFAVGAMLGQRKDKKLHAIYYASRTLDEAQRNYATTEKELLAVVFAFEKFRSYLV 1032

Query: 61   GSKVTIHSNHFAIRYLMVKKDAKSRLIR 89
            GSKV +H++H A+RYLM KKDAK RL+R
Sbjct: 1033 GSKVIVHTDHAALRYLMQKKDAKPRLLR 1060

The following BLAST results are available for this feature:

BLAST of PI0002285 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P04323	6.4e-12	45.35	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast...	[more]
P20825	3.0e-09	39.53	Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaste...	[more]
Q8I7P9	1.1e-08	33.33	Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogast...	[more]
P10394	4.7e-07	36.05	Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaste...	[more]
P10401	5.8e-05	29.89	Retrovirus-related Pol polyprotein from transposon gypsy OS=Drosophila melanogas...	[more]

BLAST of PI0002285 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7V8R9	2.1e-29	80.46	RT_RNaseH domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A5D3DS34	2.1e-29	79.55	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. mak...	[more]
A0A5A7V586	2.1e-29	79.55	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. mak...	[more]
A0A6J1DCL7	6.2e-26	65.91	LOW QUALITY PROTEIN: uncharacterized protein LOC111019515 OS=Momordica charantia...	[more]
A0A087GID7	1.1e-25	65.91	Reverse transcriptase OS=Arabis alpina OX=50452 GN=AALP_AA7G159800 PE=4 SV=1	[more]

BLAST of PI0002285 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0062414.1	4.2e-29	79.55	Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuw...	[more]
KAA0062295.1	4.2e-29	80.46	hypothetical protein E6C27_scaffold154G00380 [Cucumis melo var. makuwa] >TYK2669...	[more]
TYK26567.1	4.2e-29	79.55	Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuw...	[more]
WP_217833163.1	5.2e-27	68.18	Ty3/Gypsy family RNase HI domain-containing protein, partial [Synechococcus sp. ...	[more]
XP_019100932.1	1.2e-26	70.45	PREDICTED: uncharacterized protein LOC104789643 [Camelina sativa]	[more]

BLAST of PI0002285 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR041373	Reverse transcriptase, RNase H-like domain	PFAM	PF17917	RT_RNaseH	coord: 2..88 e-value: 3.7E-25 score: 88.3
None	No IPR available	GENE3D	3.10.20.370		coord: 1..68 e-value: 8.5E-9 score: 37.3
None	No IPR available	PANTHER	PTHR24559:SF324	TRANSPOSON TY3-I GAG-POL POLYPROTEIN-LIKE PROTEIN	coord: 1..78
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 1..78
None	No IPR available	CDD	cd09274	RNase_HI_RT_Ty3	coord: 1..88 e-value: 1.20368E-37 score: 120.29
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 1..87

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0002285.1	PI0002285.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003676	nucleic acid binding