PI0002215 (gene) Melon (PI 482460) v1

Overview


Name	PI0002215
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	cytochrome b5
Location	chr04: 17774539 .. 17777024 (+)
RNA-Seq Expression	PI0002215
Synteny	PI0002215

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAAAAAGGAGATCTTCTATCCTATGATATAGAAAATAAATAAATAAATAAAAGAAAAAGAACTGGAGGCCAATTCAATTGAAAGCAAAATAGAAAGAAAGAAAAGGTAGAACGGTTGGTACGAGAATGGCATTGCATCAGCCTTACCCCTTACCTTACCTACCAAACGCTCAATTGGAAAGAAATAAAATGATATTACCATATTAGATTCAATGTCTCACCAACCCCATTTCCCACCATTAACCTTAAAAAACTCTCTATCCCCTATTTTTTCCTTCAAAACAAGAAAGATTTGCCTCTCAGATTACACTACACTTTCAAGTTCCGACCATGGGTGGTCGCTCAGTGTTCACTTTGGCTGAGGTTGCTTCCCATGACAATCGCAACGATTGCTGGTTGATCATTGAAGACAAGGTGTTTGATCATTTTGATCATTTGTCTCTATCTCACCCCATTCTGCATTCAATTTCTTTTCTCTTCGATGGGTTTCTGTTTTTTTTCTTTTCTTTGGATCATCCTTTTCTTGTTTTCAATTTATATTGTTAAACTTTCCAATGGGTCTATTTGACTTAGACTGATTGAGTTTCATTCATGGGATTACTGTTCTGTGTTCTGTTTGAGCCTGAATTGGGATTCTGAATCCTACTTTGGTGTATTTAGACTCTGAATTTGGTCGCTTGTTAGATTCTCTGTTTCTTTTTTAGCCTTGGTTTCCAGCTTCTTTTTCTTTTTTAATGTAATTATTGGTTAGGTCTCATTTTACTTGATTGAATTGAAGTGCCTCTTCTTTTATCTTTGATGGGTGGTTTTTGCTTTTCTTTGGATCATCCTTTTCTTGTTTTTAGCATGTATGCTTACAAGTTCCAATGGGTCTATTTGATTTTGATTGATTGGGTTTCATTAATGAGATTAGTGTGAACCTGAATTGGGCTTCTGATTCTTACTAGTTTCACTGAATTTTGTGGCTTCATGTTGGGAGAGAAATAATAGATTATGTAGGAGAAAGATTGAATTCTTCTAGCAATGTTCGGCCTGTTGTTAGATTCTCCTCGATTTTTAGGTTTTTTCTTTTGTAATTAATGCCTAGGTCTCATTTTACATGACTGGAGTCCCTCCGGCTTTCTTAGAGGATTTTAGTTTTCTCAATGCCTCGATGAAAGTCAATTTTTTCATTAAATAAATAATCTACCTGTCCAAGCTCTCTGTTTTAAGAGTTTTTGACTTCCCATACATATATATGCTGCTTTTTTCATTTTAATCTAGGTATAATTCTGTGCTAGACTGGTAGATAAACAAAACTACATGTGTTTTCTTAAACCTTTTGAATTTTTACTTCTCTTTCCCATATCAAATTTGTATCCATGCTTCTTAGCTGCATATTGTTTGTGGTGAGATAACTATGGAGCAGTATGAATGTTATTTAGTTCCAAAAAGATATCCTTTAGGCCTTCATTTGGTAACCATTTGGCTTTTAGTTTTTTAGTTATGAAAAATGAAGTGTATTTCTCCCAATTTGTTACAACACTCGGCATGTTTCTTTAAGTAGAAGCATGTTTCTTTAAGTAGTAGAAGAGTGGAGTTCTTATCAAATTCTAAAAACAAAAACAAAATTTTAAAAACTACTTTTATTGTTTTTTAAATTTGGCTTAGTTTTTTTAAAACAATGGTAAAAATTAGATAACAAAGTAAGAAATTTAGTGGTAGATGAAGTGTCTATAGGCTTAATTTTTAAAAAAATACCAAAACCAAATGATCATAAATCAGGGTCGTATACTACTGATTAATTTTTGCTCAGTATCATTTTCGTTCTGTTCATTTCCCTAATCCAACAGGTGTATGATGTAACAAAATTCCTTGAAGACCATCCTGGTGGTGATGAAGTCTTGTTGTCAGGCGTTGGTATGGAAATAAACAACCATAACTTTTCATTGAAATATGCTCCTAATAAGATTTGTTTGAAATATCAATGGTGGTTCTTGACGTTATGAAATCTTGTTATCTACAGGTAAGGATGCAACCAATGATTTTTTCGATGTCGGGCACAGTAGCACTGCCAGAGCAATGATGGAAGAATTTTATGTAGGCGATATCGATAGCTCAACTATTCCTGCAAAAAGAGAGTATACTCCTCCAAAGCAACCCCTCTACAACCAAGACAAGACACCAGAATTCATTATCAAGGTCCTCCAGTTTCTTGCTCCCTTGTTAATTTTGGGCTTGGCTTTTGGCATCCACCTCTATATCAAAACAACTTAAATATTGAAACTATATCACTTCTATTTTTTTTTTTTTAGAAAATTTGGTTCAATGTTTCTCAACGAGTGTACTTTGTTGAAACTGGTATGATTATCTTGAAAACTTGTTGGGAGTATGACTATTTCTTCTATGGTTTTTTTCTCCTTGTACTTCGTTAATGCACCGACTTTTAGTATACCATGAAGGAGATGAGACTTAATAAAATTTGAGCATGTGTACCTCGCTTCCAC

mRNA sequence

AGAAAAAGGAGATCTTCTATCCTATGATATAGAAAATAAATAAATAAATAAAAGAAAAAGAACTGGAGGCCAATTCAATTGAAAGCAAAATAGAAAGAAAGAAAAGGTAGAACGGTTGGTACGAGAATGGCATTGCATCAGCCTTACCCCTTACCTTACCTACCAAACGCTCAATTGGAAAGAAATAAAATGATATTACCATATTAGATTCAATGTCTCACCAACCCCATTTCCCACCATTAACCTTAAAAAACTCTCTATCCCCTATTTTTTCCTTCAAAACAAGAAAGATTTGCCTCTCAGATTACACTACACTTTCAAGTTCCGACCATGGGTGGTCGCTCAGTGTTCACTTTGGCTGAGGTTGCTTCCCATGACAATCGCAACGATTGCTGGTTGATCATTGAAGACAAGGTGTATGATGTAACAAAATTCCTTGAAGACCATCCTGGTGGTGATGAAGTCTTGTTGTCAGGCGTTGGTAAGGATGCAACCAATGATTTTTTCGATGTCGGGCACAGTAGCACTGCCAGAGCAATGATGGAAGAATTTTATGTAGGCGATATCGATAGCTCAACTATTCCTGCAAAAAGAGAGTATACTCCTCCAAAGCAACCCCTCTACAACCAAGACAAGACACCAGAATTCATTATCAAGGTCCTCCAGTTTCTTGCTCCCTTGTTAATTTTGGGCTTGGCTTTTGGCATCCACCTCTATATCAAAACAACTTAAATATTGAAACTATATCACTTCTATTTTTTTTTTTTTAGAAAATTTGGTTCAATGTTTCTCAACGAGTGTACTTTGTTGAAACTGGTATGATTATCTTGAAAACTTGTTGGGAGTATGACTATTTCTTCTATGGTTTTTTTCTCCTTGTACTTCGTTAATGCACCGACTTTTAGTATACCATGAAGGAGATGAGACTTAATAAAATTTGAGCATGTGTACCTCGCTTCCAC

Coding sequence (CDS)

ATGGGTGGTCGCTCAGTGTTCACTTTGGCTGAGGTTGCTTCCCATGACAATCGCAACGATTGCTGGTTGATCATTGAAGACAAGGTGTATGATGTAACAAAATTCCTTGAAGACCATCCTGGTGGTGATGAAGTCTTGTTGTCAGGCGTTGGTAAGGATGCAACCAATGATTTTTTCGATGTCGGGCACAGTAGCACTGCCAGAGCAATGATGGAAGAATTTTATGTAGGCGATATCGATAGCTCAACTATTCCTGCAAAAAGAGAGTATACTCCTCCAAAGCAACCCCTCTACAACCAAGACAAGACACCAGAATTCATTATCAAGGTCCTCCAGTTTCTTGCTCCCTTGTTAATTTTGGGCTTGGCTTTTGGCATCCACCTCTATATCAAAACAACTTAA

Protein sequence

MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFDVGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLILGLAFGIHLYIKTT

Homology

[Close]

BLAST of PI0002215 vs. ExPASy Swiss-Prot
Match: P49099 (Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 1.0e-53
Identity = 99/132 (75.00%), Postives = 116/132 (87.88%), Query Frame = 0


Query: 1   MGGRS-VFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFF 60
           MGG+S VFTLAEV++H+N  DCWLII  KVY+VTKFLEDHPGG EVLLS  GKDAT+DF 
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFE 60

Query: 61  DVGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLI 120
           D+GHSS+ARAM++E+YVGDIDSSTIP K +YTPPKQP YNQDKT EFI+K+LQFL PL+I
Sbjct: 61  DIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYNQDKTTEFIVKLLQFLVPLII 120

Query: 121 LGLAFGIHLYIK 132
           LG+AFG+H Y K
Sbjct: 121 LGVAFGVHFYTK 132

[Close]

BLAST of PI0002215 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 1.5e-52
Identity = 96/132 (72.73%), Postives = 113/132 (85.61%), Query Frame = 0


Query: 1   MGGRS-VFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFF 60
           MGG + VFTLAEV+ H+N  DCWL+I  KVYDVTKFL+DHPGGDEVLLS  GKDAT+DF 
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLI 120
           DVGHSS+ARAM++E+YVGDIDS+TIP K +YTPP QP YNQDKT EF++K+LQFL PL+I
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LGLAFGIHLYIK 132
           LG+AFGI  Y K
Sbjct: 121 LGVAFGIRFYTK 132

[Close]

BLAST of PI0002215 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 201.8 bits (512), Expect = 4.7e-51
Identity = 93/129 (72.09%), Postives = 112/129 (86.82%), Query Frame = 0


Query: 4   RSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFDVGH 63
           + V+TL EVA H++++DCWLII  KVY+V+KFLEDHPGGD+VLLS  GKDAT+DF DVGH
Sbjct: 6   KKVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGH 65

Query: 64  SSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLILGLA 123
           S+TARAMM+E+YVGDID+STIPA+ +Y PPKQP YNQDKTPEFIIK+LQFL PL ILGLA
Sbjct: 66  STTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLA 125

Query: 124 FGIHLYIKT 133
             I +Y K+
Sbjct: 126 VAIRIYTKS 134

[Close]

BLAST of PI0002215 vs. ExPASy Swiss-Prot
Match: P49097 (Cytochrome b5 OS=Cuscuta reflexa OX=4129 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 1.1e-50
Identity = 94/131 (71.76%), Postives = 108/131 (82.44%), Query Frame = 0


Query: 1   MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60
           MGG  V++LAEV+ H   NDCWL+I  KVYDVTKFL+DHPGG +VLLS   KDAT+DF D
Sbjct: 1   MGGSKVYSLAEVSEHSQPNDCWLVIGGKVYDVTKFLDDHPGGADVLLSSTAKDATDDFED 60

Query: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLIL 120
           +GHSS+ARAMM+E  VGDIDSSTIP K  YTPPKQPLYNQDKTP+FIIK+LQFL PL+IL
Sbjct: 61  IGHSSSARAMMDEMCVGDIDSSTIPTKTSYTPPKQPLYNQDKTPQFIIKLLQFLVPLIIL 120

Query: 121 GLAFGIHLYIK 132
           G+A GI  Y K
Sbjct: 121 GVAVGIRFYKK 131

[Close]

BLAST of PI0002215 vs. ExPASy Swiss-Prot
Match: O04354 (Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 6.9e-50
Identity = 91/128 (71.09%), Postives = 110/128 (85.94%), Query Frame = 0


Query: 6   VFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFDVGHSS 65
           +FTLAEVA H+N  DCWLII  KVYDVTKFLEDHPGGD+VLLS  GKDAT+DF D+GHSS
Sbjct: 4   IFTLAEVAQHNNSKDCWLIINGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDIGHSS 63

Query: 66  TARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLILGLAFG 125
           +A+AM++E+YVGDIDSS+IP++ +YTPPKQPLYN DKT EF+IK+LQFL PL+IL  A G
Sbjct: 64  SAKAMLDEYYVGDIDSSSIPSQVKYTPPKQPLYNPDKTREFVIKLLQFLVPLVILAGAIG 123

Query: 126 IHLYIKTT 134
           I  Y K++
Sbjct: 124 IRFYTKSS 131

[Close]

BLAST of PI0002215 vs. ExPASy TrEMBL
Match: A0A5A7UKQ8 (Cytochrome b5, seed isoform-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1278G00070 PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 4.7e-70
Identity = 132/133 (99.25%), Postives = 133/133 (100.00%), Query Frame = 0


Query: 1   MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60
           MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD
Sbjct: 1   MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60

Query: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLIL 120
           VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPL+IL
Sbjct: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLVIL 120

Query: 121 GLAFGIHLYIKTT 134
           GLAFGIHLYIKTT
Sbjct: 121 GLAFGIHLYIKTT 133

[Close]

BLAST of PI0002215 vs. ExPASy TrEMBL
Match: A0A1S3CMW3 (cytochrome b5, seed isoform-like OS=Cucumis melo OX=3656 GN=LOC103502759 PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 4.7e-70
Identity = 132/133 (99.25%), Postives = 133/133 (100.00%), Query Frame = 0


Query: 1   MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60
           MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD
Sbjct: 1   MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60

Query: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLIL 120
           VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPL+IL
Sbjct: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLVIL 120

Query: 121 GLAFGIHLYIKTT 134
           GLAFGIHLYIKTT
Sbjct: 121 GLAFGIHLYIKTT 133

[Close]

BLAST of PI0002215 vs. ExPASy TrEMBL
Match: A0A0A0LCD6 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G627730 PE=3 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 4.0e-69
Identity = 130/133 (97.74%), Postives = 132/133 (99.25%), Query Frame = 0


Query: 1   MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60
           MGG SVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD
Sbjct: 1   MGGHSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60

Query: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLIL 120
           VGHSSTARAMMEEFYVGDIDSSTIPAKR+YTPPKQPLYNQDKTPEFIIKVLQFLAPL+IL
Sbjct: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKRDYTPPKQPLYNQDKTPEFIIKVLQFLAPLVIL 120

Query: 121 GLAFGIHLYIKTT 134
           GLAFGIHLYIKTT
Sbjct: 121 GLAFGIHLYIKTT 133

[Close]

BLAST of PI0002215 vs. ExPASy TrEMBL
Match: A0A6J1K3E7 (cytochrome b5, seed isoform-like OS=Cucurbita maxima OX=3661 GN=LOC111491329 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.3e-64
Identity = 122/133 (91.73%), Postives = 127/133 (95.49%), Query Frame = 0


Query: 1   MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60
           M G ++FTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLS +GKDATNDFFD
Sbjct: 1   MVGSTLFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60

Query: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLIL 120
           VGHSSTARAMMEEFYVGDIDSSTIP KR+YTPPKQPLYNQDKTPEFIIKVLQFLAPL+IL
Sbjct: 61  VGHSSTARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPLVIL 120

Query: 121 GLAFGIHLYIKTT 134
            LAFGIHLY KTT
Sbjct: 121 ALAFGIHLYTKTT 133

[Close]

BLAST of PI0002215 vs. ExPASy TrEMBL
Match: A0A6J1ETM5 (cytochrome b5, seed isoform-like OS=Cucurbita moschata OX=3662 GN=LOC111437450 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 3.9e-64
Identity = 120/133 (90.23%), Postives = 126/133 (94.74%), Query Frame = 0


Query: 1   MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60
           M G +VFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLS +GKDATNDFFD
Sbjct: 1   MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60

Query: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLIL 120
           VGHSS ARAMMEEFY+GDIDSSTIP KR+YTPPKQPLYNQDKTPEFIIKVLQFLAPL+IL
Sbjct: 61  VGHSSAARAMMEEFYIGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPLVIL 120

Query: 121 GLAFGIHLYIKTT 134
            LAFG+HLY KTT
Sbjct: 121 ALAFGVHLYTKTT 133

[Close]

BLAST of PI0002215 vs. NCBI nr
Match: XP_008465053.1 (PREDICTED: cytochrome b5, seed isoform-like [Cucumis melo] >KAA0054876.1 cytochrome b5, seed isoform-like [Cucumis melo var. makuwa] >TYJ96589.1 cytochrome b5, seed isoform-like [Cucumis melo var. makuwa])

HSP 1 Score: 273.5 bits (698), Expect = 9.8e-70
Identity = 132/133 (99.25%), Postives = 133/133 (100.00%), Query Frame = 0


Query: 1   MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60
           MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD
Sbjct: 1   MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60

Query: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLIL 120
           VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPL+IL
Sbjct: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLVIL 120

Query: 121 GLAFGIHLYIKTT 134
           GLAFGIHLYIKTT
Sbjct: 121 GLAFGIHLYIKTT 133

[Close]

BLAST of PI0002215 vs. NCBI nr
Match: XP_004144383.1 (cytochrome b5, seed isoform [Cucumis sativus] >KGN58362.1 hypothetical protein Csa_000942 [Cucumis sativus])

HSP 1 Score: 270.4 bits (690), Expect = 8.3e-69
Identity = 130/133 (97.74%), Postives = 132/133 (99.25%), Query Frame = 0


Query: 1   MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60
           MGG SVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD
Sbjct: 1   MGGHSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60

Query: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLIL 120
           VGHSSTARAMMEEFYVGDIDSSTIPAKR+YTPPKQPLYNQDKTPEFIIKVLQFLAPL+IL
Sbjct: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKRDYTPPKQPLYNQDKTPEFIIKVLQFLAPLVIL 120

Query: 121 GLAFGIHLYIKTT 134
           GLAFGIHLYIKTT
Sbjct: 121 GLAFGIHLYIKTT 133

[Close]

BLAST of PI0002215 vs. NCBI nr
Match: XP_038889018.1 (cytochrome b5, seed isoform isoform X3 [Benincasa hispida])

HSP 1 Score: 265.0 bits (676), Expect = 3.5e-67
Identity = 127/133 (95.49%), Postives = 130/133 (97.74%), Query Frame = 0


Query: 1   MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60
           MGGR+VF+LAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD
Sbjct: 1   MGGRTVFSLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60

Query: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLIL 120
           VGHSS ARAMMEEFYVGDIDSSTIPAKR YTPPKQPLYNQDKTPEFIIKVLQFLAPL+IL
Sbjct: 61  VGHSSAARAMMEEFYVGDIDSSTIPAKRTYTPPKQPLYNQDKTPEFIIKVLQFLAPLIIL 120

Query: 121 GLAFGIHLYIKTT 134
           GLAFGIHLY KTT
Sbjct: 121 GLAFGIHLYTKTT 133

[Close]

BLAST of PI0002215 vs. NCBI nr
Match: XP_022995981.1 (cytochrome b5, seed isoform-like [Cucurbita maxima])

HSP 1 Score: 255.4 bits (651), Expect = 2.8e-64
Identity = 122/133 (91.73%), Postives = 127/133 (95.49%), Query Frame = 0


Query: 1   MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60
           M G ++FTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLS +GKDATNDFFD
Sbjct: 1   MVGSTLFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60

Query: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLIL 120
           VGHSSTARAMMEEFYVGDIDSSTIP KR+YTPPKQPLYNQDKTPEFIIKVLQFLAPL+IL
Sbjct: 61  VGHSSTARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPLVIL 120

Query: 121 GLAFGIHLYIKTT 134
            LAFGIHLY KTT
Sbjct: 121 ALAFGIHLYTKTT 133

[Close]

BLAST of PI0002215 vs. NCBI nr
Match: XP_023534065.1 (cytochrome b5, seed isoform-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 254.6 bits (649), Expect = 4.7e-64
Identity = 122/133 (91.73%), Postives = 126/133 (94.74%), Query Frame = 0


Query: 1   MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60
           M G +VFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLS +GKDATNDFFD
Sbjct: 56  MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 115

Query: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLIL 120
           VGHSS ARAMMEEFYVGDIDSSTIP KR+YTPPKQPLYNQDKTPEFIIKVLQFLAPL+IL
Sbjct: 116 VGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPLVIL 175

Query: 121 GLAFGIHLYIKTT 134
            LAFGIHLY KTT
Sbjct: 176 ALAFGIHLYTKTT 188

[Close]

BLAST of PI0002215 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 192.6 bits (488), Expect = 2.0e-49
Identity = 88/127 (69.29%), Postives = 107/127 (84.25%), Query Frame = 0


Query: 6   VFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFDVGHSS 65
           +FTL+EV+ H+  +DCW++I  KVY+VTKFLEDHPGGD+VLLS  GKDAT+DF DVGHS 
Sbjct: 7   IFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSE 66

Query: 66  TARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLILGLAFG 125
           +AR MME++YVG+ID +TIP K +YTPPKQP YNQDKT EFIIK+LQFL PL ILGLA G
Sbjct: 67  SAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAILGLAVG 126

Query: 126 IHLYIKT 133
           I +Y K+
Sbjct: 127 IRIYTKS 133

[Close]

BLAST of PI0002215 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 184.5 bits (467), Expect = 5.6e-47
Identity = 84/128 (65.62%), Postives = 101/128 (78.91%), Query Frame = 0


Query: 4   RSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFDVGH 63
           R V +  EV+ H+   DCWLII  KVYDVT F++DHPGGDEVLLS  GKDATNDF DVGH
Sbjct: 5   RKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGH 64

Query: 64  SSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLLILGLA 123
           S TAR MM+++++G+IDSS++PA R Y  P+QP YNQDKTPEFIIK+LQFL P+LILGLA
Sbjct: 65  SDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILILGLA 124

Query: 124 FGIHLYIK 132
             +  Y K
Sbjct: 125 LVVRHYTK 132

[Close]

BLAST of PI0002215 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 182.6 bits (462), Expect = 2.1e-46
Identity = 83/133 (62.41%), Postives = 108/133 (81.20%), Query Frame = 0


Query: 2   GGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFDV 61
           G   VFTL+EV+ H +  DCW++I+ KVYDVTKFL+DHPGGDEV+L+  GKDAT+DF DV
Sbjct: 3   GDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDV 62

Query: 62  GHSSTARAMMEEFYVGDIDSSTIPAKREYTPP--KQPLYNQDKTPEFIIKVLQFLAPLLI 121
           GHSSTA+AM++E+YVGDID++T+P K ++ PP   + +  QDK+ +F+IK+LQFL PLLI
Sbjct: 63  GHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPLLI 122

Query: 122 LGLAFGIHLYIKT 133
           LGLAFGI  Y KT
Sbjct: 123 LGLAFGIRYYTKT 135

[Close]

BLAST of PI0002215 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 124.0 bits (310), Expect = 8.9e-29
Identity = 56/127 (44.09%), Postives = 86/127 (67.72%), Query Frame = 0


Query: 5   SVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFDVGHS 64
           ++ +  +VA H  +NDCW++I  KVYD++ F+++HPGGD VLL+  GKDA+ DF DV HS
Sbjct: 3   NLISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHS 62

Query: 65  STARAMMEEFYVGDIDSSTIPAKREYTPP--KQPLYNQDKTPEFIIKVLQFLAPLLILGL 124
             A+ +M+++ +GD+D ST+P  ++Y PP  K+    +    E   K+L +L PLLILG+
Sbjct: 63  KDAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGV 122

Query: 125 AFGIHLY 130
           AF +  Y
Sbjct: 123 AFALRFY 129

[Close]

BLAST of PI0002215 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 108.6 bits (270), Expect = 3.9e-24
Identity = 45/126 (35.71%), Postives = 87/126 (69.05%), Query Frame = 0


Query: 6   VFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFDVGHSS 65
           ++++ E A+H+ ++DCW++I+ KVYDV+ ++++HPGGD+VLL+  GKDAT+DF D GHS 
Sbjct: 7   LYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHSK 66

Query: 66  TARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQ-----FLAPLLIL 125
            AR +ME++++G++D S++P       P+  +Y +D+  + + K+       ++ P+ I+
Sbjct: 67  DARELMEKYFIGELDESSLP-----EIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSII 126

Query: 126 GLAFGI 127
            ++  +
Sbjct: 127 TISVAV 127

The following BLAST results are available for this feature:

BLAST of PI0002215 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P49099	1.0e-53	75.00	Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49098	1.5e-52	72.73	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49100	4.7e-51	72.09	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]
P49097	1.1e-50	71.76	Cytochrome b5 OS=Cuscuta reflexa OX=4129 PE=2 SV=1	[more]
O04354	6.9e-50	71.09	Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1	[more]

BLAST of PI0002215 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UKQ8	4.7e-70	99.25	Cytochrome b5, seed isoform-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3CMW3	4.7e-70	99.25	cytochrome b5, seed isoform-like OS=Cucumis melo OX=3656 GN=LOC103502759 PE=3 SV...	[more]
A0A0A0LCD6	4.0e-69	97.74	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A6J1K3E7	1.3e-64	91.73	cytochrome b5, seed isoform-like OS=Cucurbita maxima OX=3661 GN=LOC111491329 PE=...	[more]
A0A6J1ETM5	3.9e-64	90.23	cytochrome b5, seed isoform-like OS=Cucurbita moschata OX=3662 GN=LOC111437450 P...	[more]

BLAST of PI0002215 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008465053.1	9.8e-70	99.25	PREDICTED: cytochrome b5, seed isoform-like [Cucumis melo] >KAA0054876.1 cytochr...	[more]
XP_004144383.1	8.3e-69	97.74	cytochrome b5, seed isoform [Cucumis sativus] >KGN58362.1 hypothetical protein C...	[more]
XP_038889018.1	3.5e-67	95.49	cytochrome b5, seed isoform isoform X3 [Benincasa hispida]	[more]
XP_022995981.1	2.8e-64	91.73	cytochrome b5, seed isoform-like [Cucurbita maxima]	[more]
XP_023534065.1	4.7e-64	91.73	cytochrome b5, seed isoform-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of PI0002215 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32720.1	2.0e-49	69.29	cytochrome B5 isoform B	[more]
AT5G53560.1	5.6e-47	65.63	cytochrome B5 isoform E	[more]
AT5G48810.1	2.1e-46	62.41	cytochrome B5 isoform D	[more]
AT2G46650.1	8.9e-29	44.09	cytochrome B5 isoform C	[more]
AT1G26340.1	3.9e-24	35.71	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 39..53 score: 52.83 coord: 54..61 score: 39.58 coord: 67..79 score: 36.6 coord: 29..39 score: 70.52
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 7..80 e-value: 1.7E-32 score: 123.9
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 8..79 e-value: 5.2E-25 score: 87.4
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 4..80 score: 28.369963
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 3..85 e-value: 6.7E-34 score: 117.7
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 5..89
None	No IPR available	PANTHER	PTHR19359:SF102	SUBFAMILY NOT NAMED	coord: 1..131
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..131
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 35..42

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0002215.1	PI0002215.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding