PI0002162 (gene) Melon (PI 482460) v1

BLAST of PI0002162 vs. ExPASy Swiss-Prot
Match: Q9FRK5 (Uncharacterized membrane protein At1g75140 OS=Arabidopsis thaliana OX=3702 GN=At1g75140 PE=1 SV=3)

HSP 1 Score: 652.9 bits (1683), Expect = 3.8e-186
Identity = 360/668 (53.89%), Postives = 459/668 (68.71%), Query Frame = 0

Query: 1   MANPLKGK----FFFIYCLFVFVFPHGSRVFVYSSSSFEQNQSETE----VPFEIRRYDL 60
           MA+   GK    FFF+  + +F+ P  S V    S       S+         E  R  +
Sbjct: 1   MADSQNGKSAFFFFFVSLILLFLSPSYSDVTASESDPIPYENSDASPGVVTSSESDRQGV 60

Query: 61  -LKNLEELVRNLSDVVSKLELRLSDIPPAVNREKLNTDASRQVVLEVVSLSGGKGKEDGP 120
            L  LEELVRNL+++V++L+ +LS+ P  V +E      +R  + E              
Sbjct: 61  SLHRLEELVRNLTELVARLDAKLSETPFKVKKE-----ITRDEIEE-------------- 120

Query: 121 KEGGDDGLDYKIQDGGRTRAVSVTKYSPFWSERFHFLSAVKMESDATCINVLPLRDFEGH 180
                           + +A SVTKYSPFWSERF F SAVK++S+ATCINVLP RD EG 
Sbjct: 121 ----------------KAKAFSVTKYSPFWSERFEFTSAVKLDSEATCINVLPFRDHEGL 180

Query: 181 SKYVAVGDERGRIYVFVRNGDIAIELPTASESPITAMVSYMSIYKNETLLVTGHKNGVIL 240
           SKY AVGD  GR++VF+RNGD+ +E  T  +SPITAMVSYMS+YKNE+ +VTGH++GVIL
Sbjct: 181 SKYFAVGDSSGRVFVFLRNGDVLVEFFTTCDSPITAMVSYMSVYKNESFVVTGHQSGVIL 240

Query: 241 MHRIWEGSNGEDLNLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDLRGKIK 300
           +HR+ EGS GEDLN   ME+V +F   D  ED  Q++LLE+HHVGR RYIL++DL GK+ 
Sbjct: 241 LHRLREGSIGEDLNSAVMENVGKF---DGTEDGLQVTLLEVHHVGRVRYILATDLSGKLT 300

Query: 301 VFKEDGTVYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNY 360
           VF E+ TVYGSV PTSRP+ FLKQRLLFLTE+GAGSLDLRSMK+RESECEGLNHSLAR+Y
Sbjct: 301 VFTENRTVYGSVSPTSRPLVFLKQRLLFLTETGAGSLDLRSMKIRESECEGLNHSLARSY 360

Query: 361 VFDATERSKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLIFQTIKGYLLVTS 420
           VFDA+ER+KAYGFTS+G++IHVLLLGDIMNFKCRVRSK+K +++EP+  Q IKGYLL+ +
Sbjct: 361 VFDASERAKAYGFTSEGEIIHVLLLGDIMNFKCRVRSKKKVQMEEPVALQAIKGYLLIVN 420

Query: 421 NEKVHVFNLSSHHYVRVGAPRLLFSAGLNEIKSSFLNY----QNLDLESEGNFMPLISSD 480
            EKV V+N+S+ HYVR   PRLLF A L +I+S+FL++    +  D +      PLI+SD
Sbjct: 421 QEKVFVYNVSTQHYVRTTGPRLLFPAALEDIRSTFLSHRESTKTTDHQKLEKVTPLIASD 480

Query: 481 REKLVVLGLGGGYVGMYLSNLPTFKAEFNSMVWTSPVLFFILFLFGAWHFFAKKKEALTS 540
           REKL+V+GLG GYV  Y S LP  KAEFN+M+W+SPV FFILFLFGAWHFF+KKKE+LT+
Sbjct: 481 REKLLVMGLGDGYVATYKSKLPISKAEFNTMLWSSPVFFFILFLFGAWHFFSKKKESLTA 540

Query: 541 WGPDDPFSATSPTVAPMRTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTGY- 600
           WGPDDPFS+T+ + +   T  +  A F ++  R+ D MDLR         RRY SP+ Y 
Sbjct: 541 WGPDDPFSSTTMSSSSTTTAQNSSA-FSESTRRNDDHMDLR---------RRYVSPSRYP 600

Query: 601 PGGATSSFRPATTNEHSSRPTAVDPNYR-AASELKFR-GSPLEPPGFPKRREPLFANNQV 653
           PG AT ++R   +N+ SSR      NYR  A E+K+R GS L+  GF KRRE LF NN+ 
Sbjct: 601 PGAATGAYRSVGSNDPSSRAPVETTNYRTTAQEMKYRGGSGLDSGGFGKRRESLFGNNKA 617

BLAST of PI0002162 vs. ExPASy TrEMBL
Match: A0A1S3BAX2 (uncharacterized membrane protein At1g75140-like OS=Cucumis melo OX=3656 GN=LOC103487958 PE=4 SV=1)

HSP 1 Score: 1151.3 bits (2977), Expect = 0.0e+00
Identity = 591/657 (89.95%), Postives = 614/657 (93.46%), Query Frame = 0

Query: 1   MANPLKGKFFFIYCLFVFVFPHGSRVFVYSSSSFEQNQSETEVPFEIRRYDLLKNLEELV 60
           MA   KG+FFFI+ LF+F FPH SRVFV SS+SFEQNQSE EVPFEI++YDLLKNLEELV
Sbjct: 1   MAIERKGRFFFIFFLFIFAFPHVSRVFVCSSNSFEQNQSEIEVPFEIKQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPPAVNREKLNTDASRQVVLEVVSLSGGKGKEDGPKEGGDDGLD 120
           RNLSDVVSKLELRLSDIP AVNREKLN DASR+VV E V    GKGKED P    D GLD
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVNREKLNIDASRRVVPE-VGKEKGKGKEDDP---NDVGLD 120

Query: 121 YKIQDGGRTRAVSVTKYSPFWSERFHFLSAVKMESDATCINVLPLRDFEGHSKYVAVGDE 180
           +KIQDGGRTRAVSVTKYS FWSERFHFLSAVK+ESDATC NVLPLRDFEG+SKYVAVGDE
Sbjct: 121 HKIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCTNVLPLRDFEGNSKYVAVGDE 180

Query: 181 RGRIYVFVRNGDIAIELPTASESPITAMVSYMSIYKNETLLVTGHKNGVILMHRIWEGSN 240
           RGRIYVFVRNGD+AIELPT   SPITAM+SYMSIYKNETLLVTGHKNG ILMHRIWEGSN
Sbjct: 181 RGRIYVFVRNGDVAIELPTVPGSPITAMLSYMSIYKNETLLVTGHKNGGILMHRIWEGSN 240

Query: 241 GEDLNLIFMEHVVEFVATDSREDE--SQISLLELHHVGRTRYILSSDLRGKIKVFKEDGT 300
           GEDLNLIFME VVEFVATDSREDE  SQISLLELHHVGRTRYILSSDLRGKIKVFKEDGT
Sbjct: 241 GEDLNLIFMERVVEFVATDSREDESQSQISLLELHHVGRTRYILSSDLRGKIKVFKEDGT 300

Query: 301 VYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATER 360
           VYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSL RNYVFDATER
Sbjct: 301 VYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLVRNYVFDATER 360

Query: 361 SKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLIFQTIKGYLLVTSNEKVHVF 420
           SKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPL FQTIKGYLLVTSNEKVHVF
Sbjct: 361 SKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVF 420

Query: 421 NLSSHHYVRVGAPRLLFSAGLNEIKSSFLNYQNLDLESEGNF-MPLISSDREKLVVLGLG 480
           N+SSHHYVRVGAPRLLFSAGL+EIKSSFLNYQNLDLESE NF +PLISSDREKLVVLGLG
Sbjct: 421 NVSSHHYVRVGAPRLLFSAGLDEIKSSFLNYQNLDLESEANFYIPLISSDREKLVVLGLG 480

Query: 481 GGYVGMYLSNLPTFKAEFNSMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFSAT 540
           GGYVGMY SNLP FK EFN+M+WTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPF+AT
Sbjct: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT 540

Query: 541 SPTV-APMRTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTGYPGGATSSFRP 600
           SPT  APM TGSSERASFIDTPSRSTD+MDLRGGGGLRGP RRYGSPTGYPGGATSSFRP
Sbjct: 541 SPTTGAPMGTGSSERASFIDTPSRSTDVMDLRGGGGLRGPARRYGSPTGYPGGATSSFRP 600

Query: 601 ATTNEHSSRPTAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDESS 654
           ATT++HSSRP AVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVD+S+
Sbjct: 601 ATTSDHSSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDSN 653

BLAST of PI0002162 vs. ExPASy TrEMBL
Match: A0A0A0LNK9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G361500 PE=4 SV=1)

HSP 1 Score: 1149.0 bits (2971), Expect = 0.0e+00
Identity = 583/654 (89.14%), Postives = 607/654 (92.81%), Query Frame = 0

Query: 1   MANPLKGKFFFIYCLFVFVFPHGSRVFVYSSSSFEQNQSETEVPFEIRRYDLLKNLEELV 60
           MA   KG+FFFI+ LF+F FPH SRVFV SSSS EQNQSE EVPFEI++YDLLKNLEELV
Sbjct: 1   MAIERKGRFFFIFFLFIFAFPHVSRVFVCSSSSLEQNQSEIEVPFEIKQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPPAVNREKLNTDASRQVVLEVVSLSGGKGKEDGPKEGGDDGLD 120
           RNLSDVVSKLELRLSDIP AVNREKLNTDA R+VV E V     KGKE+G  +GGD GLD
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVNREKLNTDAFRRVVPE-VGKEKRKGKEEGLNDGGDGGLD 120

Query: 121 YKIQDGGRTRAVSVTKYSPFWSERFHFLSAVKMESDATCINVLPLRDFEGHSKYVAVGDE 180
           +KIQDGGRTRAVSVTKYS FWSERFHFLSAVK+E+DATCINVLPLRDFEGHSKYVAVGDE
Sbjct: 121 HKIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLEADATCINVLPLRDFEGHSKYVAVGDE 180

Query: 181 RGRIYVFVRNGDIAIELPTASESPITAMVSYMSIYKNETLLVTGHKNGVILMHRIWEGSN 240
           RGRIYVFV NGD+AIELPT   SP+TAM+SYMSIYKNETLLVTGHKNG ILMHRIWEGSN
Sbjct: 181 RGRIYVFVINGDVAIELPTVPGSPVTAMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSN 240

Query: 241 GEDLNLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDLRGKIKVFKEDGTVY 300
           GEDLNLIFMEHVVEFVAT+SREDESQISLLELHHVGRTRYILSSD  GKIKVF+EDGTVY
Sbjct: 241 GEDLNLIFMEHVVEFVATNSREDESQISLLELHHVGRTRYILSSDFGGKIKVFREDGTVY 300

Query: 301 GSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATERSK 360
           GSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDA ERSK
Sbjct: 301 GSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDAMERSK 360

Query: 361 AYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLIFQTIKGYLLVTSNEKVHVFNL 420
           AYG TSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPL FQTIKGYLLVTSNEKVHVFN+
Sbjct: 361 AYGVTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFNV 420

Query: 421 SSHHYVRVGAPRLLFSAGLNEIKSSFLNYQNLDLESEGNFMPLISSDREKLVVLGLGGGY 480
           SS HYVRVGAPRLLFSAGL+EIKSSFLNYQN DL SE NF+PLISSD EKLVVLGLGGGY
Sbjct: 421 SSQHYVRVGAPRLLFSAGLDEIKSSFLNYQNSDLVSEANFIPLISSDHEKLVVLGLGGGY 480

Query: 481 VGMYLSNLPTFKAEFNSMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFSATSPT 540
           VGMY SNLP FK EFN+MVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPF+ATSPT
Sbjct: 481 VGMYRSNLPIFKGEFNTMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATSPT 540

Query: 541 V-APMRTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTGYPGGATSSFRPATT 600
             APM TGSSERASFIDTPSRSTD+MDLR  GGLRGPPRRYGSPTGYP GATSSFRPATT
Sbjct: 541 TGAPMGTGSSERASFIDTPSRSTDVMDLRSAGGLRGPPRRYGSPTGYPAGATSSFRPATT 600

Query: 601 NEHSSRPTAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDESS 654
           N+HSSRP AVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVD+SS
Sbjct: 601 NDHSSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDSS 653

BLAST of PI0002162 vs. ExPASy TrEMBL
Match: A0A5A7VC92 (Putative membrane protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005890 PE=4 SV=1)

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 571/630 (90.63%), Postives = 592/630 (93.97%), Query Frame = 0

Query: 30  SSSSFEQNQSETEVPFEIRRYDLLKNLEELVRNLSDVVSKLELRLSDIPPAVNREKLNTD 89
           SS+SFEQNQSE EVPFEI++YDLLKNLEELVRNLSDVVSKLELRLSDIP AVNREKLN D
Sbjct: 8   SSNSFEQNQSEIEVPFEIKQYDLLKNLEELVRNLSDVVSKLELRLSDIPTAVNREKLNID 67

Query: 90  ASRQVVLEVVSLSGGKGKEDGPKEGGDDGLDYKIQDGGRTRAVSVTKYSPFWSERFHFLS 149
           ASR+VV E V    GKGKED P    D GLD+KIQDGGRTRAVSVTKYS FWSERFHFLS
Sbjct: 68  ASRRVVPE-VGKEKGKGKEDDP---NDVGLDHKIQDGGRTRAVSVTKYSSFWSERFHFLS 127

Query: 150 AVKMESDATCINVLPLRDFEGHSKYVAVGDERGRIYVFVRNGDIAIELPTASESPITAMV 209
           AVK+ESDATCINVLPLRDFEG+SKYVAVGDERGRIYVFVRNGD+AIELPT   SPITAM+
Sbjct: 128 AVKLESDATCINVLPLRDFEGNSKYVAVGDERGRIYVFVRNGDVAIELPTVPGSPITAML 187

Query: 210 SYMSIYKNETLLVTGHKNGVILMHRIWEGSNGEDLNLIFMEHVVEFVATDSREDE--SQI 269
           SYMSIYKNETLLVTGHKNG ILMHRIWEGSNGEDLNLIFME +VEFVATDSREDE  SQI
Sbjct: 188 SYMSIYKNETLLVTGHKNGGILMHRIWEGSNGEDLNLIFME-LVEFVATDSREDESQSQI 247

Query: 270 SLLELHHVGRTRYILSSDLRGKIKVFKEDGTVYGSVMPTSRPIAFLKQRLLFLTESGAGS 329
           SLLELHHVGRTRYILSSDLRGKIKVFKEDGTVYGSVMPTSRPIAFLKQRLLFLTESGAGS
Sbjct: 248 SLLELHHVGRTRYILSSDLRGKIKVFKEDGTVYGSVMPTSRPIAFLKQRLLFLTESGAGS 307

Query: 330 LDLRSMKLRESECEGLNHSLARNYVFDATERSKAYGFTSDGDLIHVLLLGDIMNFKCRVR 389
           LDLRSMKLRESECEGLNHSL RNYVFDATERSKAYGFTSDGDLIHVLLLGDIMNFKCRVR
Sbjct: 308 LDLRSMKLRESECEGLNHSLVRNYVFDATERSKAYGFTSDGDLIHVLLLGDIMNFKCRVR 367

Query: 390 SKRKFELDEPLIFQTIKGYLLVTSNEKVHVFNLSSHHYVRVGAPRLLFSAGLNEIKSSFL 449
           SKRKFELDEPL FQTIKGYLLVTSNEKVHVFN+SSHHYVRVGAPRLLFSAGL+EIKSSFL
Sbjct: 368 SKRKFELDEPLAFQTIKGYLLVTSNEKVHVFNVSSHHYVRVGAPRLLFSAGLDEIKSSFL 427

Query: 450 NYQN--LDLESEGNF-MPLISSDREKLVVLGLGGGYVGMYLSNLPTFKAEFNSMVWTSPV 509
           NYQN  LDLESE NF +PLISSDREKLVVLGLG GYVGMY SNLP FK EFN+M+WTSPV
Sbjct: 428 NYQNLDLDLESEANFYIPLISSDREKLVVLGLGAGYVGMYRSNLPIFKGEFNTMLWTSPV 487

Query: 510 LFFILFLFGAWHFFAKKKEALTSWGPDDPFSATSPTV-APMRTGSSERASFIDTPSRSTD 569
           LFFILFLFGAWHFFAKKKEALTSWGPDDPF+ATSPT  APM TGSSERASFIDTPSRSTD
Sbjct: 488 LFFILFLFGAWHFFAKKKEALTSWGPDDPFTATSPTTGAPMGTGSSERASFIDTPSRSTD 547

Query: 570 MMDLRGGGGLRGPPRRYGSPTGYPGGATSSFRPATTNEHSSRPTAVDPNYRAASELKFRG 629
           +MDLRGGGGLRGP RRYGSPTGYPGGATSSFRPATT++HSSRP AVDPNYRAASELKFRG
Sbjct: 548 VMDLRGGGGLRGPARRYGSPTGYPGGATSSFRPATTSDHSSRPAAVDPNYRAASELKFRG 607

Query: 630 SPLEPPGFPKRREPLFANNQVVNQVVDESS 654
           SPLEPPGFPKRREPLFANNQVVNQVVD+S+
Sbjct: 608 SPLEPPGFPKRREPLFANNQVVNQVVDDSN 632

BLAST of PI0002162 vs. ExPASy TrEMBL
Match: A0A0A0LNL2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G361540 PE=4 SV=1)

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 564/652 (86.50%), Postives = 588/652 (90.18%), Query Frame = 0

Query: 1   MANPLKGKFFFIYCLFVFVFPHGSRVFVYSSSSFEQNQSETEVPFEIRRYDLLKNLEELV 60
           MANP KGKFFFIYCLFVFVFPH SRVFVYSSSSFEQNQSETEVPFEIR+YDLLKNLEELV
Sbjct: 1   MANPRKGKFFFIYCLFVFVFPHCSRVFVYSSSSFEQNQSETEVPFEIRQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPPAVNREKLNTDASRQVVLEVVSLSGGKGKEDGPKEGGDDGLD 120
           RNLSDVVSKLEL LSDIP  VNREKLNTD                        GGDDGLD
Sbjct: 61  RNLSDVVSKLELMLSDIPAVVNREKLNTD------------------------GGDDGLD 120

Query: 121 YKIQDGGRTRAVSVTKYSPFWSERFHFLSAVKMESDATCINVLPLRDFEGHSKYVAVGDE 180
             IQ  GR RAVSVTKYS FWSERFHFLSAVK+ESDATCINVLPLRDFEGHSKYVAVGDE
Sbjct: 121 NSIQAEGRMRAVSVTKYSLFWSERFHFLSAVKLESDATCINVLPLRDFEGHSKYVAVGDE 180

Query: 181 RGRIYVFVRNGDIAIELPTASESPITAMVSYMSIYKNETLLVTGHKNGVILMHRIWEGSN 240
           RGRIYVFV NGD+AIELPT   SP+TAM+SYMSIYKNET+LVTGHKNG ILMHRIWEGSN
Sbjct: 181 RGRIYVFVINGDVAIELPTVPGSPVTAMLSYMSIYKNETILVTGHKNGGILMHRIWEGSN 240

Query: 241 GEDLNLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDLRGKIKVFKEDGTVY 300
           GEDLNLIFMEHVVEFVATDSREDESQISLLELH+VGRTRYILSSD RGKIKVFKEDGTVY
Sbjct: 241 GEDLNLIFMEHVVEFVATDSREDESQISLLELHYVGRTRYILSSDFRGKIKVFKEDGTVY 300

Query: 301 GSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATERSK 360
           GSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLA +YVFDATERSK
Sbjct: 301 GSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLALSYVFDATERSK 360

Query: 361 AYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLIFQTIKGYLLVTSNEKVHVFNL 420
           AYGFTSDGDLIHVLLLGDIMNFKCRVRSKRK EL++PL+FQTIKGYLLV+SNEKVHVFN+
Sbjct: 361 AYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKLELNKPLMFQTIKGYLLVSSNEKVHVFNV 420

Query: 421 SSHHYVRVGAPRLLFSAGLNEIKSSFLNYQNLDLESEGNFMPLISSDREKLVVLGLGGGY 480
           SS HYVRVGAPRLLFSA L+EIKSSFLNYQNLDLES  NFMPLISSDREKLVVLGLG GY
Sbjct: 421 SSQHYVRVGAPRLLFSAALDEIKSSFLNYQNLDLES--NFMPLISSDREKLVVLGLGEGY 480

Query: 481 VGMYLSNLPTFKAEFNSMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFSATSPT 540
           VGMY SNLP F+ EFNS VWTSPVLFF LFLFGAWHFF+KKKEA TSWGPD+PFSATSPT
Sbjct: 481 VGMYHSNLPIFRGEFNSTVWTSPVLFFTLFLFGAWHFFSKKKEAFTSWGPDEPFSATSPT 540

Query: 541 -VAPMRTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTGYPGGATSSFRPATT 600
            VAPM T S+ R+SF DTPSRSTDMMD+RGGGGLRGPPRRYGSPT YP GAT+SFRPATT
Sbjct: 541 SVAPMGTVSNNRSSFTDTPSRSTDMMDIRGGGGLRGPPRRYGSPTRYPVGATTSFRPATT 600

Query: 601 NEHSSRPTAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDE 652
           N HSS  +A+DPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVV+QVVDE
Sbjct: 601 NNHSSSRSAIDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVSQVVDE 626

BLAST of PI0002162 vs. ExPASy TrEMBL
Match: A0A6J1KBU3 (uncharacterized membrane protein At1g75140-like OS=Cucurbita maxima OX=3661 GN=LOC111491879 PE=4 SV=1)

HSP 1 Score: 1086.6 bits (2809), Expect = 0.0e+00
Identity = 556/669 (83.11%), Postives = 597/669 (89.24%), Query Frame = 0

Query: 1   MANPLKGKFFFIYCLFVFVFPHGSRVFVYSSSSFEQNQSETEVPFEIRRYDLLKNLEELV 60
           MANP KGK  FIY LFVF  PH SR FV  SSSFE NQSETEVPFE R+YDLLKNLEELV
Sbjct: 1   MANPRKGKLLFIYFLFVFGIPHVSRDFVCCSSSFELNQSETEVPFETRQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPPAVNRE-KLNTDASRQVVLEVVSLSGGKGKEDGPKEGGDDGL 120
           RNLSDVVSKLELRLS+IP AV+R+ KLNTDASR+VV EVVSL G  GKEDG  +GGD  L
Sbjct: 61  RNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSLGG--GKEDGLNDGGDGAL 120

Query: 121 DYKIQDGGRTRAVSVTKYSPFWSERFHFLSAVKMESDATCINVLPLRDFEGHSKYVAVGD 180
           D KIQDGGR RAVSVTKYSPFWSERFHFLSAVK+ESDAT INVLPLRDFEGHSKYVAVGD
Sbjct: 121 DTKIQDGGRGRAVSVTKYSPFWSERFHFLSAVKLESDATSINVLPLRDFEGHSKYVAVGD 180

Query: 181 ERGRIYVFVRNGDIAIELPTASESPITAMVSYMSIYKNETLLVTGHKNGVILMHRIWEGS 240
           E GRIYVFVRNGD+A+EL T S SPITA +SYMSIYKNETLLVTGH++G I M RIWEGS
Sbjct: 181 ETGRIYVFVRNGDVAVELRTMSGSPITATLSYMSIYKNETLLVTGHESGAISMLRIWEGS 240

Query: 241 NGEDLNLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDLRGKIKVFKEDGTV 300
           NGE+ N IFMEHV+EFV TD RE++S IS+LE+HHVGRTRYILSSD++GKIKVF+EDGTV
Sbjct: 241 NGEESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVFREDGTV 300

Query: 301 YGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATERS 360
           YGSVMPTSRPIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLN+SLARNYVFDATERS
Sbjct: 301 YGSVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERS 360

Query: 361 KAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLIFQTIKGYLLVTSNEKVHVFN 420
           KAYGFTSDGDLIHV+LLGDIMNFKCRVRSK+KFELDEPL FQ +KGYLLV  +EKVHVFN
Sbjct: 361 KAYGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLFFQAVKGYLLVIGSEKVHVFN 420

Query: 421 LSSHHYVRVGAPRLLFSAGLNEIKSSFLNYQNLDLESEGNFMPLISSDREKLVVLGLGGG 480
           +SS HYVRVGAPRLLFSA L+EI+SSFLN QNLDLES+G F+PLISSDREKLVVLGLGGG
Sbjct: 421 VSSQHYVRVGAPRLLFSARLDEIRSSFLNNQNLDLESQGKFIPLISSDREKLVVLGLGGG 480

Query: 481 YVGMYLSNLPTFKAEFNSMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFSATSP 540
           Y+GMY SNLP +K EFN+M+WTSPVLFFILFLFGAW FFAKKKEALTSWGPDDPF+ATSP
Sbjct: 481 YIGMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATSP 540

Query: 541 TV-APMRTGSSE--------------RASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPT 600
           T  AP+ TGS+E              R SFIDTPSRSTDMMDLRGG GLRGPPRRYGSPT
Sbjct: 541 TTGAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPT 600

Query: 601 GYPGGATSSFRPATTNEHSSRPTAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQV 654
           GYPGG TSSFRPATT++HSSR  AVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQV
Sbjct: 601 GYPGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQV 660

BLAST of PI0002162 vs. NCBI nr
Match: XP_011649613.2 (uncharacterized membrane protein At1g75140 [Cucumis sativus] >KAE8652103.1 hypothetical protein Csa_022548 [Cucumis sativus])

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 584/654 (89.30%), Postives = 608/654 (92.97%), Query Frame = 0

Query: 1   MANPLKGKFFFIYCLFVFVFPHGSRVFVYSSSSFEQNQSETEVPFEIRRYDLLKNLEELV 60
           MA   KG+FFFI+ LF+F FPH SRVFV SSSS EQNQSE EVPFEI++YDLLKNLEELV
Sbjct: 1   MAIERKGRFFFIFFLFIFAFPHVSRVFVCSSSSLEQNQSEIEVPFEIKQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPPAVNREKLNTDASRQVVLEVVSLSGGKGKEDGPKEGGDDGLD 120
           RNLSDVVSKLELRLSDIP AVNREKLNTDA R+VV E V     KGKE+G  +GGD GLD
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVNREKLNTDAFRRVVPE-VGKEKRKGKEEGLNDGGDGGLD 120

Query: 121 YKIQDGGRTRAVSVTKYSPFWSERFHFLSAVKMESDATCINVLPLRDFEGHSKYVAVGDE 180
           +KIQDGGRTRAVSVTKYS FWSERFHFLSAVK+E+DATCINVLPLRDFEGHSKYVAVGDE
Sbjct: 121 HKIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLEADATCINVLPLRDFEGHSKYVAVGDE 180

Query: 181 RGRIYVFVRNGDIAIELPTASESPITAMVSYMSIYKNETLLVTGHKNGVILMHRIWEGSN 240
           RGRIYVFVRNGD+AIELPT   SP+TAM+SYMSIYKNETLLVTGHKNG ILMHRIWEGSN
Sbjct: 181 RGRIYVFVRNGDVAIELPTVPGSPVTAMLSYMSIYKNETLLVTGHKNGAILMHRIWEGSN 240

Query: 241 GEDLNLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDLRGKIKVFKEDGTVY 300
           GEDLNLIFMEHVVEFVAT+SREDESQISLLELHHVGRTRYILSSD  GKIKVF+EDGTVY
Sbjct: 241 GEDLNLIFMEHVVEFVATNSREDESQISLLELHHVGRTRYILSSDFGGKIKVFREDGTVY 300

Query: 301 GSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATERSK 360
           GSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDA ERSK
Sbjct: 301 GSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDAMERSK 360

Query: 361 AYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLIFQTIKGYLLVTSNEKVHVFNL 420
           AYG TSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPL FQTIKGYLLVTSNEKVHVFN+
Sbjct: 361 AYGVTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVFNV 420

Query: 421 SSHHYVRVGAPRLLFSAGLNEIKSSFLNYQNLDLESEGNFMPLISSDREKLVVLGLGGGY 480
           SS HYVRVGAPRLLFSAGL+EIKSSFLNYQN DL SE NF+PLISSD EKLVVLGLGGGY
Sbjct: 421 SSQHYVRVGAPRLLFSAGLDEIKSSFLNYQNSDLVSEANFIPLISSDHEKLVVLGLGGGY 480

Query: 481 VGMYLSNLPTFKAEFNSMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFSATSPT 540
           VGMY SNLP FK EFN+MVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPF+ATSPT
Sbjct: 481 VGMYRSNLPIFKGEFNTMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTATSPT 540

Query: 541 V-APMRTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTGYPGGATSSFRPATT 600
             APM TGSSERASFIDTPSRSTD+MDLR  GGLRGPPRRYGSPTGYP GATSSFRPATT
Sbjct: 541 TGAPMGTGSSERASFIDTPSRSTDVMDLRSAGGLRGPPRRYGSPTGYPAGATSSFRPATT 600

Query: 601 NEHSSRPTAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDESS 654
           N+HSSRP AVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVD+SS
Sbjct: 601 NDHSSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDSS 653

BLAST of PI0002162 vs. NCBI nr
Match: XP_008444699.1 (PREDICTED: uncharacterized membrane protein At1g75140-like [Cucumis melo])

HSP 1 Score: 1151.3 bits (2977), Expect = 0.0e+00
Identity = 591/657 (89.95%), Postives = 614/657 (93.46%), Query Frame = 0

Query: 1   MANPLKGKFFFIYCLFVFVFPHGSRVFVYSSSSFEQNQSETEVPFEIRRYDLLKNLEELV 60
           MA   KG+FFFI+ LF+F FPH SRVFV SS+SFEQNQSE EVPFEI++YDLLKNLEELV
Sbjct: 1   MAIERKGRFFFIFFLFIFAFPHVSRVFVCSSNSFEQNQSEIEVPFEIKQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPPAVNREKLNTDASRQVVLEVVSLSGGKGKEDGPKEGGDDGLD 120
           RNLSDVVSKLELRLSDIP AVNREKLN DASR+VV E V    GKGKED P    D GLD
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVNREKLNIDASRRVVPE-VGKEKGKGKEDDP---NDVGLD 120

Query: 121 YKIQDGGRTRAVSVTKYSPFWSERFHFLSAVKMESDATCINVLPLRDFEGHSKYVAVGDE 180
           +KIQDGGRTRAVSVTKYS FWSERFHFLSAVK+ESDATC NVLPLRDFEG+SKYVAVGDE
Sbjct: 121 HKIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCTNVLPLRDFEGNSKYVAVGDE 180

Query: 181 RGRIYVFVRNGDIAIELPTASESPITAMVSYMSIYKNETLLVTGHKNGVILMHRIWEGSN 240
           RGRIYVFVRNGD+AIELPT   SPITAM+SYMSIYKNETLLVTGHKNG ILMHRIWEGSN
Sbjct: 181 RGRIYVFVRNGDVAIELPTVPGSPITAMLSYMSIYKNETLLVTGHKNGGILMHRIWEGSN 240

Query: 241 GEDLNLIFMEHVVEFVATDSREDE--SQISLLELHHVGRTRYILSSDLRGKIKVFKEDGT 300
           GEDLNLIFME VVEFVATDSREDE  SQISLLELHHVGRTRYILSSDLRGKIKVFKEDGT
Sbjct: 241 GEDLNLIFMERVVEFVATDSREDESQSQISLLELHHVGRTRYILSSDLRGKIKVFKEDGT 300

Query: 301 VYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATER 360
           VYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSL RNYVFDATER
Sbjct: 301 VYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLVRNYVFDATER 360

Query: 361 SKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLIFQTIKGYLLVTSNEKVHVF 420
           SKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPL FQTIKGYLLVTSNEKVHVF
Sbjct: 361 SKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEKVHVF 420

Query: 421 NLSSHHYVRVGAPRLLFSAGLNEIKSSFLNYQNLDLESEGNF-MPLISSDREKLVVLGLG 480
           N+SSHHYVRVGAPRLLFSAGL+EIKSSFLNYQNLDLESE NF +PLISSDREKLVVLGLG
Sbjct: 421 NVSSHHYVRVGAPRLLFSAGLDEIKSSFLNYQNLDLESEANFYIPLISSDREKLVVLGLG 480

Query: 481 GGYVGMYLSNLPTFKAEFNSMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFSAT 540
           GGYVGMY SNLP FK EFN+M+WTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPF+AT
Sbjct: 481 GGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFTAT 540

Query: 541 SPTV-APMRTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTGYPGGATSSFRP 600
           SPT  APM TGSSERASFIDTPSRSTD+MDLRGGGGLRGP RRYGSPTGYPGGATSSFRP
Sbjct: 541 SPTTGAPMGTGSSERASFIDTPSRSTDVMDLRGGGGLRGPARRYGSPTGYPGGATSSFRP 600

Query: 601 ATTNEHSSRPTAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDESS 654
           ATT++HSSRP AVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVD+S+
Sbjct: 601 ATTSDHSSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDDSN 653

BLAST of PI0002162 vs. NCBI nr
Match: XP_038885478.1 (uncharacterized membrane protein At1g75140-like [Benincasa hispida])

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 572/656 (87.20%), Postives = 605/656 (92.23%), Query Frame = 0

Query: 1   MANPLKGKFFFIYCLFVFVFPHGSRVFVYSSSSFEQNQSETEVPFEIRRYDLLKNLEELV 60
           MAN  KGK  FIY LFVF FPH SRVFV SSSSFE NQSE EVPFEI++Y+LLKNLEELV
Sbjct: 1   MANLRKGKLLFIYFLFVFSFPHVSRVFVCSSSSFELNQSEIEVPFEIKQYNLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPPAVNREKLNTDASRQVVLEVVSLSG--GKGKEDGPKEGGDDG 120
           RNLSDVVSKLELRLSDIP AV+++KLNTDASR+VV EVV L G   KGKEDG  +GGD G
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVHKDKLNTDASRRVVSEVVGLGGVKRKGKEDGLNDGGDGG 120

Query: 121 LDYKIQDGGRTRAVSVTKYSPFWSERFHFLSAVKMESDATCINVLPLRDFEGHSKYVAVG 180
           LD KIQDGGR RAVSVTKYSPFWSERFHFLSAVK+ESDATCINVLP+RDFEGHSKYVAVG
Sbjct: 121 LDNKIQDGGRMRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPIRDFEGHSKYVAVG 180

Query: 181 DERGRIYVFVRNGDIAIELPTASESPITAMVSYMSIYKNETLLVTGHKNGVILMHRIWEG 240
           DE+GRIYVFVRNGD+AIELPT SESPITAM+SY SIYKNETLLVTGH NG ILMHRIWEG
Sbjct: 181 DEKGRIYVFVRNGDVAIELPTVSESPITAMLSYTSIYKNETLLVTGHNNGAILMHRIWEG 240

Query: 241 SNGEDLNLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDLRGKIKVFKEDGT 300
            NGE+LN IFMEHVVEFVATDSRED+S IS+LE+HHVGRTRYILSSD RGKIKVFKEDGT
Sbjct: 241 LNGEELNSIFMEHVVEFVATDSREDDSPISILEVHHVGRTRYILSSDFRGKIKVFKEDGT 300

Query: 301 VYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATER 360
           VYGSV   SRPIAFLKQRLLFLTESGAGSLDLRSMKLRE+ CEGLN+SLARNYVFDATER
Sbjct: 301 VYGSVTLMSRPIAFLKQRLLFLTESGAGSLDLRSMKLREAGCEGLNYSLARNYVFDATER 360

Query: 361 SKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLIFQTIKGYLLVTSNEKVHVF 420
           SKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPL+FQ IKGYLLV SNEKVHVF
Sbjct: 361 SKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEKVHVF 420

Query: 421 NLSSHHYVRVGAPRLLFSAGLNEIKSSFLNYQNLDLESEGNFMPLISSDREKLVVLGLGG 480
           N+SS HYVRV APRLLFSAGL+EI+SSFLNYQNL+LESEG F+PLISSD EKLVVLGLGG
Sbjct: 421 NVSSQHYVRVAAPRLLFSAGLDEIRSSFLNYQNLNLESEGKFIPLISSDHEKLVVLGLGG 480

Query: 481 GYVGMYLSNLPTFKAEFNSMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFSATS 540
           GYVGMY SNLP FK EFNSM+WTSPVLFFILFLFGAW FFAKKKEALTSWGPDDPF+ATS
Sbjct: 481 GYVGMYRSNLPIFKGEFNSMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPFTATS 540

Query: 541 PTV-APMRTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTGYPGGATSSFRPA 600
           PT   P+ TGSSERASFIDTPSRSTDMMDLRGGGG+RGPPR YGSPTGYPGGATSSFRP 
Sbjct: 541 PTTGVPLGTGSSERASFIDTPSRSTDMMDLRGGGGIRGPPRHYGSPTGYPGGATSSFRPP 600

Query: 601 TTNEHSSRPTAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDESS 654
           TT++HSSRP AVDP+YRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQ+VDESS
Sbjct: 601 TTSDHSSRPAAVDPSYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQMVDESS 656

BLAST of PI0002162 vs. NCBI nr
Match: XP_038885660.1 (uncharacterized membrane protein At1g75140-like [Benincasa hispida])

HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 559/654 (85.47%), Postives = 595/654 (90.98%), Query Frame = 0

Query: 1   MANPLKGKFFFIYCLFVFVFPHGSRVFVYSSSSFEQNQSETEVPFEIRRYDLLKNLEELV 60
           MANP KGK  FIY L VF F H SRV V SSSSFE NQS  EVPFE+R+YDLLKNLEELV
Sbjct: 1   MANPRKGKLLFIYLLLVFAFLHVSRVVVCSSSSFELNQSGIEVPFEVRQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPPAVNREKLNTDASRQVVLEVVSLSGGKGKEDGPKEGGDDGLD 120
           RNLSDVVSKLELRLSDIP  V REKLN DASR+V+ E  +L+          +GGD GLD
Sbjct: 61  RNLSDVVSKLELRLSDIPTVVRREKLNPDASRRVMPEGDTLN----------DGGDGGLD 120

Query: 121 YKIQDGGRTRAVSVTKYSPFWSERFHFLSAVKMESDATCINVLPLRDFEGHSKYVAVGDE 180
            KIQDG R RAVSVTKYSPFWSERFHFLSAVK+ESDATCINVLPLRDFEG+SKYVAVGDE
Sbjct: 121 NKIQDGSRARAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPLRDFEGNSKYVAVGDE 180

Query: 181 RGRIYVFVRNGDIAIELPTASESPITAMVSYMSIYKNETLLVTGHKNGVILMHRIWEGSN 240
           +GRIYVF+R+GD+AIELPT SESPITAM+SYMSIYKNETLLVTGHKNG+ILMHRIWEGSN
Sbjct: 181 KGRIYVFMRSGDVAIELPTVSESPITAMLSYMSIYKNETLLVTGHKNGMILMHRIWEGSN 240

Query: 241 GEDLNLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDLRGKIKVFKEDGTVY 300
           GE+LNLIFMEHVVEF  TDS+EDES IS+LE+HHVG TRYILSSD RG IKVFKEDGTVY
Sbjct: 241 GEELNLIFMEHVVEFADTDSQEDESPISILEVHHVGWTRYILSSDFRGVIKVFKEDGTVY 300

Query: 301 GSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFDATERSK 360
           GSVMP SRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLN+SLARNYVFDATERSK
Sbjct: 301 GSVMPMSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFDATERSK 360

Query: 361 AYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLIFQTIKGYLLVTSNEKVHVFNL 420
           AYGFTSDGDLIHVLLLGDIMNFKCR+RSKRKFELD PL+FQ IKGYLLV SNEKVH+FN+
Sbjct: 361 AYGFTSDGDLIHVLLLGDIMNFKCRIRSKRKFELDGPLVFQAIKGYLLVISNEKVHIFNV 420

Query: 421 SSHHYVRVGAPRLLFSAGLNEIKSSFLNYQNLDLESEGNFMPLISSDREKLVVLGLGGGY 480
           SS HYVRVGAPRLLFSAGL+EI SSFLNYQNLDLESEGNF+PLISSDREKLVVLGLGGGY
Sbjct: 421 SSQHYVRVGAPRLLFSAGLDEIMSSFLNYQNLDLESEGNFIPLISSDREKLVVLGLGGGY 480

Query: 481 VGMYLSNLPTFKAEFNSMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPFSATSPT 540
           VGMY SNLP FK EFN+M+WTSPVLFFILFLFGAWH FAKKKEALTSWGPDD F+ATSPT
Sbjct: 481 VGMYRSNLPVFKGEFNTMLWTSPVLFFILFLFGAWHLFAKKKEALTSWGPDDSFTATSPT 540

Query: 541 V-APMRTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTGYPGGATSSFRPATT 600
             AP+ TGSSERASFID PSR+TDMMDLRGG GLRGPPRRYGSPTGYPGGATSSFRPATT
Sbjct: 541 TGAPLGTGSSERASFIDPPSRTTDMMDLRGGAGLRGPPRRYGSPTGYPGGATSSFRPATT 600

Query: 601 NEHSSRPTAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQVVDESS 654
           ++HSSRP AVDPNYRAASE+KFRGSPLEPPGFPKRREPLFANNQVVNQVV E++
Sbjct: 601 SDHSSRPAAVDPNYRAASEIKFRGSPLEPPGFPKRREPLFANNQVVNQVVAENN 644

BLAST of PI0002162 vs. NCBI nr
Match: KAA0065248.1 (putative membrane protein [Cucumis melo var. makuwa])

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 571/630 (90.63%), Postives = 592/630 (93.97%), Query Frame = 0

Query: 30  SSSSFEQNQSETEVPFEIRRYDLLKNLEELVRNLSDVVSKLELRLSDIPPAVNREKLNTD 89
           SS+SFEQNQSE EVPFEI++YDLLKNLEELVRNLSDVVSKLELRLSDIP AVNREKLN D
Sbjct: 8   SSNSFEQNQSEIEVPFEIKQYDLLKNLEELVRNLSDVVSKLELRLSDIPTAVNREKLNID 67

Query: 90  ASRQVVLEVVSLSGGKGKEDGPKEGGDDGLDYKIQDGGRTRAVSVTKYSPFWSERFHFLS 149
           ASR+VV E V    GKGKED P    D GLD+KIQDGGRTRAVSVTKYS FWSERFHFLS
Sbjct: 68  ASRRVVPE-VGKEKGKGKEDDP---NDVGLDHKIQDGGRTRAVSVTKYSSFWSERFHFLS 127

Query: 150 AVKMESDATCINVLPLRDFEGHSKYVAVGDERGRIYVFVRNGDIAIELPTASESPITAMV 209
           AVK+ESDATCINVLPLRDFEG+SKYVAVGDERGRIYVFVRNGD+AIELPT   SPITAM+
Sbjct: 128 AVKLESDATCINVLPLRDFEGNSKYVAVGDERGRIYVFVRNGDVAIELPTVPGSPITAML 187

Query: 210 SYMSIYKNETLLVTGHKNGVILMHRIWEGSNGEDLNLIFMEHVVEFVATDSREDE--SQI 269
           SYMSIYKNETLLVTGHKNG ILMHRIWEGSNGEDLNLIFME +VEFVATDSREDE  SQI
Sbjct: 188 SYMSIYKNETLLVTGHKNGGILMHRIWEGSNGEDLNLIFME-LVEFVATDSREDESQSQI 247

Query: 270 SLLELHHVGRTRYILSSDLRGKIKVFKEDGTVYGSVMPTSRPIAFLKQRLLFLTESGAGS 329
           SLLELHHVGRTRYILSSDLRGKIKVFKEDGTVYGSVMPTSRPIAFLKQRLLFLTESGAGS
Sbjct: 248 SLLELHHVGRTRYILSSDLRGKIKVFKEDGTVYGSVMPTSRPIAFLKQRLLFLTESGAGS 307

Query: 330 LDLRSMKLRESECEGLNHSLARNYVFDATERSKAYGFTSDGDLIHVLLLGDIMNFKCRVR 389
           LDLRSMKLRESECEGLNHSL RNYVFDATERSKAYGFTSDGDLIHVLLLGDIMNFKCRVR
Sbjct: 308 LDLRSMKLRESECEGLNHSLVRNYVFDATERSKAYGFTSDGDLIHVLLLGDIMNFKCRVR 367

Query: 390 SKRKFELDEPLIFQTIKGYLLVTSNEKVHVFNLSSHHYVRVGAPRLLFSAGLNEIKSSFL 449
           SKRKFELDEPL FQTIKGYLLVTSNEKVHVFN+SSHHYVRVGAPRLLFSAGL+EIKSSFL
Sbjct: 368 SKRKFELDEPLAFQTIKGYLLVTSNEKVHVFNVSSHHYVRVGAPRLLFSAGLDEIKSSFL 427

Query: 450 NYQN--LDLESEGNF-MPLISSDREKLVVLGLGGGYVGMYLSNLPTFKAEFNSMVWTSPV 509
           NYQN  LDLESE NF +PLISSDREKLVVLGLG GYVGMY SNLP FK EFN+M+WTSPV
Sbjct: 428 NYQNLDLDLESEANFYIPLISSDREKLVVLGLGAGYVGMYRSNLPIFKGEFNTMLWTSPV 487

Query: 510 LFFILFLFGAWHFFAKKKEALTSWGPDDPFSATSPTV-APMRTGSSERASFIDTPSRSTD 569
           LFFILFLFGAWHFFAKKKEALTSWGPDDPF+ATSPT  APM TGSSERASFIDTPSRSTD
Sbjct: 488 LFFILFLFGAWHFFAKKKEALTSWGPDDPFTATSPTTGAPMGTGSSERASFIDTPSRSTD 547

Query: 570 MMDLRGGGGLRGPPRRYGSPTGYPGGATSSFRPATTNEHSSRPTAVDPNYRAASELKFRG 629
           +MDLRGGGGLRGP RRYGSPTGYPGGATSSFRPATT++HSSRP AVDPNYRAASELKFRG
Sbjct: 548 VMDLRGGGGLRGPARRYGSPTGYPGGATSSFRPATTSDHSSRPAAVDPNYRAASELKFRG 607

Query: 630 SPLEPPGFPKRREPLFANNQVVNQVVDESS 654
           SPLEPPGFPKRREPLFANNQVVNQVVD+S+
Sbjct: 608 SPLEPPGFPKRREPLFANNQVVNQVVDDSN 632

BLAST of PI0002162 vs. TAIR 10
Match: AT1G75140.1 (unknown protein; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G19370.1); Has 51 Blast hits to 49 proteins in 18 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 5 (source: NCBI BLink). )

HSP 1 Score: 652.9 bits (1683), Expect = 2.7e-187
Identity = 360/668 (53.89%), Postives = 459/668 (68.71%), Query Frame = 0

Query: 1   MANPLKGK----FFFIYCLFVFVFPHGSRVFVYSSSSFEQNQSETE----VPFEIRRYDL 60
           MA+   GK    FFF+  + +F+ P  S V    S       S+         E  R  +
Sbjct: 1   MADSQNGKSAFFFFFVSLILLFLSPSYSDVTASESDPIPYENSDASPGVVTSSESDRQGV 60

Query: 61  -LKNLEELVRNLSDVVSKLELRLSDIPPAVNREKLNTDASRQVVLEVVSLSGGKGKEDGP 120
            L  LEELVRNL+++V++L+ +LS+ P  V +E      +R  + E              
Sbjct: 61  SLHRLEELVRNLTELVARLDAKLSETPFKVKKE-----ITRDEIEE-------------- 120

Query: 121 KEGGDDGLDYKIQDGGRTRAVSVTKYSPFWSERFHFLSAVKMESDATCINVLPLRDFEGH 180
                           + +A SVTKYSPFWSERF F SAVK++S+ATCINVLP RD EG 
Sbjct: 121 ----------------KAKAFSVTKYSPFWSERFEFTSAVKLDSEATCINVLPFRDHEGL 180

Query: 181 SKYVAVGDERGRIYVFVRNGDIAIELPTASESPITAMVSYMSIYKNETLLVTGHKNGVIL 240
           SKY AVGD  GR++VF+RNGD+ +E  T  +SPITAMVSYMS+YKNE+ +VTGH++GVIL
Sbjct: 181 SKYFAVGDSSGRVFVFLRNGDVLVEFFTTCDSPITAMVSYMSVYKNESFVVTGHQSGVIL 240

Query: 241 MHRIWEGSNGEDLNLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDLRGKIK 300
           +HR+ EGS GEDLN   ME+V +F   D  ED  Q++LLE+HHVGR RYIL++DL GK+ 
Sbjct: 241 LHRLREGSIGEDLNSAVMENVGKF---DGTEDGLQVTLLEVHHVGRVRYILATDLSGKLT 300

Query: 301 VFKEDGTVYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNY 360
           VF E+ TVYGSV PTSRP+ FLKQRLLFLTE+GAGSLDLRSMK+RESECEGLNHSLAR+Y
Sbjct: 301 VFTENRTVYGSVSPTSRPLVFLKQRLLFLTETGAGSLDLRSMKIRESECEGLNHSLARSY 360

Query: 361 VFDATERSKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLIFQTIKGYLLVTS 420
           VFDA+ER+KAYGFTS+G++IHVLLLGDIMNFKCRVRSK+K +++EP+  Q IKGYLL+ +
Sbjct: 361 VFDASERAKAYGFTSEGEIIHVLLLGDIMNFKCRVRSKKKVQMEEPVALQAIKGYLLIVN 420

Query: 421 NEKVHVFNLSSHHYVRVGAPRLLFSAGLNEIKSSFLNY----QNLDLESEGNFMPLISSD 480
            EKV V+N+S+ HYVR   PRLLF A L +I+S+FL++    +  D +      PLI+SD
Sbjct: 421 QEKVFVYNVSTQHYVRTTGPRLLFPAALEDIRSTFLSHRESTKTTDHQKLEKVTPLIASD 480

Query: 481 REKLVVLGLGGGYVGMYLSNLPTFKAEFNSMVWTSPVLFFILFLFGAWHFFAKKKEALTS 540
           REKL+V+GLG GYV  Y S LP  KAEFN+M+W+SPV FFILFLFGAWHFF+KKKE+LT+
Sbjct: 481 REKLLVMGLGDGYVATYKSKLPISKAEFNTMLWSSPVFFFILFLFGAWHFFSKKKESLTA 540

Query: 541 WGPDDPFSATSPTVAPMRTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTGY- 600
           WGPDDPFS+T+ + +   T  +  A F ++  R+ D MDLR         RRY SP+ Y 
Sbjct: 541 WGPDDPFSSTTMSSSSTTTAQNSSA-FSESTRRNDDHMDLR---------RRYVSPSRYP 600

Query: 601 PGGATSSFRPATTNEHSSRPTAVDPNYR-AASELKFR-GSPLEPPGFPKRREPLFANNQV 653
           PG AT ++R   +N+ SSR      NYR  A E+K+R GS L+  GF KRRE LF NN+ 
Sbjct: 601 PGAATGAYRSVGSNDPSSRAPVETTNYRTTAQEMKYRGGSGLDSGGFGKRRESLFGNNKA 617

BLAST of PI0002162 vs. TAIR 10
Match: AT1G19370.1 (unknown protein; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G75140.1); Has 45 Blast hits to 43 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 44; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 636.3 bits (1640), Expect = 2.6e-182
Identity = 348/658 (52.89%), Postives = 444/658 (67.48%), Query Frame = 0

Query: 1   MANPLKGKFFFIYCLFVFVFPHGSRVFVYSSSSFEQNQSETEVPFEI----RRYDLLKNL 60
           MA+PL GK FFI    +F F       +Y++ S       T+V   +    R   LL NL
Sbjct: 1   MADPLNGKSFFICFSLLFSFTLLFISPLYATESPVIEDVSTDVAVSVSETNREAVLLHNL 60

Query: 61  EELVRNLSDVVSKLELRLSDIPPAVNREKLNTDASRQVVLEVVSLSGGKGKEDGPKEGGD 120
           EELV+NL+++V+ L+ +LS  P     E              +S+    G+E        
Sbjct: 61  EELVKNLTELVANLDAKLSATPLKEKNE--------------ISVDDDIGEE-------- 120

Query: 121 DGLDYKIQDGGRTRAVSVTKYSPFWSERFHFLSAVKMESDATCINVLPLRDFEGHSKYVA 180
                  ++ GR +A SVTKYSPFWSERF F SAVK+ SDATCINVLP RDFEG SKY A
Sbjct: 121 -------KERGRAKAFSVTKYSPFWSERFQFTSAVKLNSDATCINVLPFRDFEGSSKYFA 180

Query: 181 VGDERGRIYVFVRNGDIAIELPTASESPITAMVSYMSIYKNETLLVTGHKNGVILMHRIW 240
           +GD +GR+YVF+RNGD+ IE  T  +SP+TAMVSY S++KN + +VTGH+NG +L+HRI 
Sbjct: 181 IGDSKGRVYVFLRNGDVLIEFFTTVDSPVTAMVSYSSVFKNSSFVVTGHQNGAVLLHRIH 240

Query: 241 EGSNGED--LNLIFMEHVVEFVATDSREDESQISLLELHHVGRTRYILSSDLRGKIKVFK 300
           EGSNGED   N + MEHV +F   DS +    ++LLE+HHVGR RYIL++DL GK+ V  
Sbjct: 241 EGSNGEDWNSNSVSMEHVGKFDVDDSAD---PVTLLEVHHVGRVRYILATDLSGKLTVLT 300

Query: 301 EDGTVYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFD 360
           E+ TVYGSV+P+SRP+ FLKQRLLFLTESGAGSLDLRSMK+RE+ECEGLNHSLAR YVFD
Sbjct: 301 ENRTVYGSVIPSSRPLVFLKQRLLFLTESGAGSLDLRSMKIRETECEGLNHSLARTYVFD 360

Query: 361 ATERSKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLIFQTIKGYLLVTSNEK 420
           A ERSKAYGFTS+G++IHVLL GDIMNFKCRVRSK+KF+++EP+  Q+IKGYLLV + EK
Sbjct: 361 AAERSKAYGFTSEGEIIHVLLHGDIMNFKCRVRSKKKFQMEEPVALQSIKGYLLVINEEK 420

Query: 421 VHVFNLSSHHYVRVGAPRLLFSAGLNEIKSSFLNYQNLDLESEG--NFMPLISSDREKLV 480
           V  FN+S+ HYVR   PRLLFSAGL EI+S+FL+++     +       PLI+SDRE L+
Sbjct: 421 VFAFNVSTQHYVRTAGPRLLFSAGLEEIRSAFLSHRESSSRTTTVVKTRPLIASDRENLL 480

Query: 481 VLGLGGGYVGMYLSNLPTFKAEFNSMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDD 540
           V+GL  GY  +Y S LPT K +FN+M+W+SPV FFILFLFGAWHFFAKKKE+LT+WGPDD
Sbjct: 481 VIGLENGYFAVYKSKLPTLKGDFNTMLWSSPVFFFILFLFGAWHFFAKKKESLTAWGPDD 540

Query: 541 PFSATSPTVAPMRTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTGYPGGATS 600
           PF+ T       +  S++  +F +   R+ D+MDLR         RRY         A  
Sbjct: 541 PFTPTG-----AQNSSAKEPTFTEPSRRNDDLMDLR---------RRY---------AGG 600

Query: 601 SFRPATTNEHSSRPTAVDPNYRAASE----LKFRGSPLEPPGFPKRREPLFANNQVVN 647
           S+R    N+ SSR   VD NYR  ++     +  GS L+  GF  RR+ LF NN+V++
Sbjct: 601 SYRSVGANDPSSR-APVDGNYRTTAQDHNNYRGGGSGLDSNGFGNRRDHLFGNNKVLD 602