PI0001376 (gene) Melon (PI 482460) v1

BLAST of PI0001376 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 1.0e-111
Identity = 234/537 (43.58%), Postives = 334/537 (62.20%), Query Frame = 0

Query: 1   MFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDT 60
           +F+ GL  FE MG  A   +L+ Y +  MHF L  AAN +TNF+G+ F+ +LLGG+LSD 
Sbjct: 49  LFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTIFIFALLGGYLSDA 108

Query: 61  YINRLTTCLIFGFLEIIALILITIQAYSHDLLP---SPLCPKDC--VKGRIAFVFYTSLY 120
           ++    T +IFGF+E+   IL+++QA+   L P   +PL  + C   KG  A +F+ +LY
Sbjct: 109 FLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNPLIDQTCEEAKGFKAMIFFMALY 168

Query: 121 LLAIGSGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVN 180
           L+A+GSG V+  + A GADQF+Q  PK++K L ++FN    +  +G  + +TL+VWV  +
Sbjct: 169 LVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTH 228

Query: 181 KAWYWGFFISALAATVGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLLLPDTP 240
                GF +SA A T+G I    G  ++R + P +S    +  VIV AI  R+L  P  P
Sbjct: 229 SGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKLASPSDP 288

Query: 241 NEL---YEISDKLYMDSIHYKIVHTNQLRFLDKAAIVPKD--IEPQPWNVCSVTQVEEVK 300
             L   + +++ +   S    + HT + RFLDKA I  +D   +  PW +C+VTQVE+VK
Sbjct: 289 RMLHGDHHVANDVVPSS---TLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQVEQVK 348

Query: 301 IITRMVPIFISTIIMNTCLAQLQTFSVEQG---NTRIMDKNLGHFQFPAPSIPVIPLVFM 360
            +  +VPIF STI+ NT LAQLQTFSV+QG   NTR+ +     F  P  S+  IP + +
Sbjct: 349 TLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNTRLSNS----FHIPPASLQAIPYIML 408

Query: 361 AFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATKHP 420
            FL+PLY+   VPFARK+T H SGI  L R+G+GL LS  +M  A ++E KRR  +    
Sbjct: 409 IFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVL-D 468

Query: 421 DEPMSLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLSSI 480
              +S+FW+  Q+ IFGI++MFT VGL+EFFYK++  GM S   + T+ S +FG+Y SS+
Sbjct: 469 GRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSV 528

Query: 481 FVNVVNKVTKKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSILNFFHYLYWASW 525
            V+VVNK+T     S KGW   L   DLN + L+LFYW LA+LS+LNF  YL+W+ W
Sbjct: 529 LVSVVNKITSTSVDS-KGW---LGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573

BLAST of PI0001376 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.1e-108
Identity = 227/542 (41.88%), Postives = 330/542 (60.89%), Query Frame = 0

Query: 2   FIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTY 61
           F+  +   E++ F+AN ++LV Y    MH  L  +++ +T FM +AFLL+LLGGFL+D +
Sbjct: 36  FVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAF 95

Query: 62  INRLTTCLIFGFLEIIALILITIQAYSHDLLPSPLCPK-----DCVKGRIAFVFYTSLYL 121
            +     LI   +E + LIL+TIQA    L+P P         + V G  A   +  LYL
Sbjct: 96  FSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSAALRCEVVGGSKAAFLFVGLYL 155

Query: 122 LAIGSGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNK 181
           +++G GG++G+LP+ GA+QF++  PK  K   TFFNY +  +  GA V VT +VW+  NK
Sbjct: 156 VSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNK 215

Query: 182 AWYWGFFISALAATVGFIIFAIGKPFYRIQVPGQSPILRVIQVI----VVAIKNRRLLLP 241
            W WGF +S ++  +  ++F +G  FY+ ++P  SP+  + +V+    +V+  ++     
Sbjct: 216 GWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNH 275

Query: 242 DTPNELY-EISDKLYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKI 301
            T  E+  E  +K    S+      TN L  L+KA    +      W  C+V QVE+VKI
Sbjct: 276 FTSREVQSEHEEKTPSQSL------TNSLTCLNKAI---EGKTHHIWLECTVQQVEDVKI 335

Query: 302 ITRMVPIFISTIIMNTCLAQLQTFSVEQGNTRIMDKNLGHFQFPAPSIPVIPLVFMAFLI 361
           + +M+PIF  TI++N CLAQL T+SV Q  T  M++ + +F  P+ S+PV P+VFM  L 
Sbjct: 336 VLKMLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFPVVFMLILA 395

Query: 362 PLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATK------ 421
           P Y+ + +PFARK+T    GIT LQR+GVGLVLS +AM VA LVE+KR+  A +      
Sbjct: 396 PTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDS 455

Query: 422 HPDEPMSLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLS 481
               P++  W+A QY   G AD+FTL GLLEFF+ EAP  MRSL+TS ++ SLA GYYLS
Sbjct: 456 EETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLS 515

Query: 482 SIFVNVVNKVTKKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSILNFFHYLYWASWY 528
           S+ V +VN+VTK  +  +  W    + E LN N L+LFYW + +LS++NF HYL+WA  Y
Sbjct: 516 SVMVPIVNRVTK--SAGQSPW----LGEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRY 560

BLAST of PI0001376 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 2.4e-105
Identity = 225/560 (40.18%), Postives = 332/560 (59.29%), Query Frame = 0

Query: 2   FIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTY 61
           F+  +   E++ ++AN ++LV Y    MH     +AN +TNFMG+AFLL+LLGGFLSD +
Sbjct: 34  FVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLGGFLSDAF 93

Query: 62  INRLTTCLIFGFLEIIALILITIQAYSHDLLP----SPLCPKDCVKGRIAFVFYTSLYLL 121
            +     LI   +E + LI++TIQA +  L+P    SP C +  V G  A + +  LYL+
Sbjct: 94  FSTFQIFLISASIEFLGLIILTIQARTPSLMPPSCDSPTCEE--VSGSKAAMLFVGLYLV 153

Query: 122 AIGSGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKA 181
           A+G GG++G+L + GA+QF++  PK  K   TFFNY +  +  GA V VT +VW+  NK 
Sbjct: 154 ALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKG 213

Query: 182 WYWGFFISALAATVGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVA---------IKNRR 241
           W WGF +S +A  V  +IF  G  FYR ++P  SP+  +++V++ A           N  
Sbjct: 214 WEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSGSSSNAV 273

Query: 242 LLLPDTPN------------ELYEISDKLYMDSIHYKIVHTNQLRFLDKAAIVPKDIEP- 301
             +  +P+               E+      +++  +   TN L+ L+ AA    D +P 
Sbjct: 274 ASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAA----DEKPV 333

Query: 302 QPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQLQTFSVEQGNTRIMDKNLGHFQFP 361
                C+V QVE+VKI+ +M+PIF  TI++N CLAQL TFSV+Q  +  M+  +G  + P
Sbjct: 334 HRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAAS--MNTKIGSLKIP 393

Query: 362 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLV 421
             S+P+ P+VF+  L P+Y+ + +PFARK T   +G+T LQR+GVGLVLS +AM VA LV
Sbjct: 394 PASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALV 453

Query: 422 EVKRRHQA------TKHPDEPMSLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSL 481
           E+KR+  A            P++  W+A QY   G AD+FTL GLLE+F+ EAP  MRSL
Sbjct: 454 EIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSL 513

Query: 482 STSFTFLSLAFGYYLSSIFVNVVNKVTKKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAI 530
           +TS ++ SLA GYYLSS+ V++VN +T   +     W+ G   + +N   L+ FYW + +
Sbjct: 514 ATSLSWASLAMGYYLSSVIVSIVNSITG--SSGNTPWLRG---KSINRYKLDYFYWLMCV 573

BLAST of PI0001376 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 3.2e-105
Identity = 229/537 (42.64%), Postives = 326/537 (60.71%), Query Frame = 0

Query: 1   MFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDT 60
           +F+ G   FE M   A   +L+ Y    MHF L  +AN +TNF+G+ FLLSLLGGFLSD+
Sbjct: 46  LFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSLLGGFLSDS 105

Query: 61  YINRLTTCLIFGFLEIIALILITIQAYSHDLLPSPLCPKD----CVK--GRIAFVFYTSL 120
           Y+    T L+FG +EI   IL+++QA+  +L P     K     CV+  G  A   YT+L
Sbjct: 106 YLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYKAATLYTAL 165

Query: 121 YLLAIGSGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAV 180
            L+A+GSG ++  + + GA+QF +KD    + L +FFN    +  +G  + +TL+VWV  
Sbjct: 166 CLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIALTLLVWVQT 225

Query: 181 NKAWYWGFFISALAATVGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLLLPDT 240
           +     GF +SA     G I    G  FYR + P  S    + QV V AI  R+ + P  
Sbjct: 226 HSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITKRKQICPSN 285

Query: 241 PNELYEISDKLYMDSIHYK-IVHTNQLRFLDKAAIVPK-DIEPQPWNVCSVTQVEEVKII 300
           PN +++ S     D +  K ++H+N+ RFLDKA I  +      PW +C++ QV +VKI+
Sbjct: 286 PNMVHQPS----TDLVRVKPLLHSNKFRFLDKACIKTQGKAMESPWRLCTIEQVHQVKIL 345

Query: 301 TRMVPIFISTIIMNTCLAQLQTFSVEQG---NTRIMDKNLGHFQFPAPSIPVIPLVFMAF 360
             ++PIF  TII NT LAQLQTFSV+QG   NT I       FQ P  S+  IP + + F
Sbjct: 346 LSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKT----FQIPPASLQAIPYIILIF 405

Query: 361 LIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATKHPDE 420
            +PLYE  FVP ARK+T + SGI+ LQR+G GL L+  +M  A LVE KRR ++    + 
Sbjct: 406 FVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRR-ESFLEQNV 465

Query: 421 PMSLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLSSIFV 480
            +S+FW+A Q+ IFG+++MFT VGL+EFFYK++   M+S  T+ T+ S +FG+YLSS+ V
Sbjct: 466 MLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSVLV 525

Query: 481 NVVNKVTKKI-TPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSILNFFHYLYWASWY 526
           + VN+VT    + +K+GW   L   DLN + L+ FYW LA LS +NFF+YL+W+ WY
Sbjct: 526 STVNRVTSSNGSGTKEGW---LGDNDLNKDRLDHFYWLLASLSFINFFNYLFWSRWY 567

BLAST of PI0001376 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.2e-96
Identity = 206/539 (38.22%), Postives = 313/539 (58.07%), Query Frame = 0

Query: 2   FIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTY 61
           FI G    E + +    T+LV Y    ++    TAAN +TN+ G+ ++  L+G F++D Y
Sbjct: 33  FILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNVTNWSGTCYITPLIGAFIADAY 92

Query: 62  INRLTTCLIFGFLEIIALILITIQAYSHDLLPSPLCPKDC-VKGRIAFVFYTSLYLLAIG 121
           + R  T   F F+ +  + L+T+ A    L P       C        VF+ +LY++A+G
Sbjct: 93  LGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCNADTCHPNSSQTAVFFVALYMIALG 152

Query: 122 SGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYW 181
           +GG++  + + GADQF++ D  E     +FFN+   S+ +GA +  T++VW+ +N  W W
Sbjct: 153 TGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGW 212

Query: 182 GFFISALAATVGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLLLPDTPNELYE 241
           GF +  +A  +    F  G  FYR+Q PG SP+ R+ QVIV A +   + +P+  + L+E
Sbjct: 213 GFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFE 272

Query: 242 ISDKLYMDSIHYKIVHTNQLRFLDKAAIVP-----KDIEPQPWNVCSVTQVEEVKIITRM 301
            +D         K+VHT+ L+F DKAA+       KD E  PW +CSVTQVEE+K I  +
Sbjct: 273 TADDESNIKGSRKLVHTDNLKFFDKAAVESQSDSIKDGEVNPWRLCSVTQVEELKSIITL 332

Query: 302 VPIFISTIIMNTCLAQLQTFSVEQGNTRIMDKNLG-HFQFPAPSIPVIPLVFMAFLIPLY 361
           +P++ + I+  T  +Q+ T  V QGNT  MD+++G +F+ P+ S+ +   V + F  P+Y
Sbjct: 333 LPVWATGIVFATVYSQMSTMFVLQGNT--MDQHMGKNFEIPSASLSLFDTVSVLFWTPVY 392

Query: 362 EFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKR-----RHQATKHPDE 421
           +   +P ARK T +  G TQLQR+G+GLV+S  AM  AG++EV R      H A      
Sbjct: 393 DQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMITAGVLEVVRLDYVKTHNAYDQKQI 452

Query: 422 PMSLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLSSIFV 481
            MS+FW   QY + G A++FT +G LEFFY +AP  MRSL ++ +  ++A G YLS++ V
Sbjct: 453 HMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLV 512

Query: 482 NVVNKVTKKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSILNFFHYLYWASWYKYK 529
            VV K+TKK    K GW    IP++LN  +L+ F++ LA LS LNF  YL+ +  YKYK
Sbjct: 513 TVVMKITKK--NGKPGW----IPDNLNRGHLDYFFYLLATLSFLNFLVYLWISKRYKYK 563

BLAST of PI0001376 vs. ExPASy TrEMBL
Match: A0A1S4E0E0 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucumis melo OX=3656 GN=LOC103493841 PE=3 SV=1)

HSP 1 Score: 1166.8 bits (3017), Expect = 0.0e+00
Identity = 597/641 (93.14%), Postives = 612/641 (95.48%), Query Frame = 0

Query: 1   MFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDT 60
           MFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDT
Sbjct: 40  MFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDT 99

Query: 61  YINRLTTCLIFGFLEIIALILITIQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIG 120
           YINRLTTCLIFGFLE+IALILIT+QAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIG
Sbjct: 100 YINRLTTCLIFGFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIG 159

Query: 121 SGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYW 180
           SGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVV+GAAVGVT+IVWVAVNKAWYW
Sbjct: 160 SGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVLGAAVGVTVIVWVAVNKAWYW 219

Query: 181 GFFISALAATVGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLLLPDTPNELYE 240
           GFFISALAA VGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRL LPDTPNELYE
Sbjct: 220 GFFISALAALVGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLRLPDTPNELYE 279

Query: 241 ISDKLYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFI 300
           ISDK+YMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFI
Sbjct: 280 ISDKVYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFI 339

Query: 301 STIIMNTCLAQLQTFSVEQGNTRIMDKNLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVP 360
           STIIMNTCLAQLQTFSVEQGNT IMDK+LGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVP
Sbjct: 340 STIIMNTCLAQLQTFSVEQGNTIIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVP 399

Query: 361 FARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATKHPDEPMSLFWLAFQY 420
           FARKITHHPSGITQLQRVGVGLVLSAIAM VAGLVEVKRRHQA KHPDEP+SLFWLAFQY
Sbjct: 400 FARKITHHPSGITQLQRVGVGLVLSAIAMAVAGLVEVKRRHQAIKHPDEPISLFWLAFQY 459

Query: 421 GIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLSSIFVNVVNKVTKKIT 480
           GIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLA GYYLSSIFVN+VNKVT+ IT
Sbjct: 460 GIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNIVNKVTRNIT 519

Query: 481 PSKKGWVEGLIPEDLNHNNLNLFYWFLAILSILNFFHYLYWASWYKYKTEEPILELNGKG 540
           PSK+GWVEGLIPEDLNHNNLNLFYWFLAILS+LNFFHYLYWASWYKYKTEEPI ELN KG
Sbjct: 520 PSKRGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPIEELNSKG 579

Query: 541 GET----------------SKEPIAELNKGGKEIKEPIAELNGRGEGETQEPNAELNGGR 600
           GET                +KEPIAELNK G+EIKEP AELNGRGEGET+EPN ELNGGR
Sbjct: 580 GETTKEPIAELNSKEGETMTKEPIAELNKQGEEIKEPTAELNGRGEGETKEPNVELNGGR 639

Query: 601 ETKEPIAESNERGGDDVPILHKEGNGEDAKTHHSEEKEANL 626
           ETKEPI ESNERGGDDVPILHKEGNGEDAKTHHSEEKE+NL
Sbjct: 640 ETKEPITESNERGGDDVPILHKEGNGEDAKTHHSEEKESNL 680

BLAST of PI0001376 vs. ExPASy TrEMBL
Match: A0A5A7ULM1 (Protein NRT1/ PTR FAMILY 4.5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold323G00540 PE=3 SV=1)

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 596/641 (92.98%), Postives = 612/641 (95.48%), Query Frame = 0

Query: 1   MFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDT 60
           MFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDT
Sbjct: 25  MFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDT 84

Query: 61  YINRLTTCLIFGFLEIIALILITIQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIG 120
           YINRLTTCLIFGFLE+IALILIT+QAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIG
Sbjct: 85  YINRLTTCLIFGFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIG 144

Query: 121 SGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYW 180
           SGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVV+GAAVGVT+IVWVAVNKAWYW
Sbjct: 145 SGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVLGAAVGVTVIVWVAVNKAWYW 204

Query: 181 GFFISALAATVGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLLLPDTPNELYE 240
           GFFISALAA VGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRL LPDTPNELYE
Sbjct: 205 GFFISALAALVGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLRLPDTPNELYE 264

Query: 241 ISDKLYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFI 300
           ISDK+YMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFI
Sbjct: 265 ISDKVYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFI 324

Query: 301 STIIMNTCLAQLQTFSVEQGNTRIMDKNLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVP 360
           STIIMNTCLAQLQTFSVEQGNT IMDK+LGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVP
Sbjct: 325 STIIMNTCLAQLQTFSVEQGNTIIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVP 384

Query: 361 FARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATKHPDEPMSLFWLAFQY 420
           FARKITHHPSGITQLQRVGVGLVLSAIAM VAGLVEVKRRHQA KHPDEP+SLFWLAFQY
Sbjct: 385 FARKITHHPSGITQLQRVGVGLVLSAIAMAVAGLVEVKRRHQAIKHPDEPISLFWLAFQY 444

Query: 421 GIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLSSIFVNVVNKVTKKIT 480
           GIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLA GYYLSSIFVN+VNKVT+ IT
Sbjct: 445 GIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNIVNKVTRNIT 504

Query: 481 PSKKGWVEGLIPEDLNHNNLNLFYWFLAILSILNFFHYLYWASWYKYKTEEPILELNGKG 540
           PSK+GWVEGLIPEDLNHNNLNLFYWFLAILS+LNFFHYLYWASWYKYKTEEPI ELN +G
Sbjct: 505 PSKRGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPIEELNSRG 564

Query: 541 GET----------------SKEPIAELNKGGKEIKEPIAELNGRGEGETQEPNAELNGGR 600
           GET                +KEPIAELNK G+EIKEP AELNGRGEGET+EPN ELNGGR
Sbjct: 565 GETTKEPIAELNSKEGETMTKEPIAELNKQGEEIKEPTAELNGRGEGETKEPNVELNGGR 624

Query: 601 ETKEPIAESNERGGDDVPILHKEGNGEDAKTHHSEEKEANL 626
           ETKEPI ESNERGGDDVPILHKEGNGEDAKTHHSEEKE+NL
Sbjct: 625 ETKEPITESNERGGDDVPILHKEGNGEDAKTHHSEEKESNL 665

BLAST of PI0001376 vs. ExPASy TrEMBL
Match: A0A5D3BBU1 (Protein NRT1/ PTR FAMILY 4.5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G00540 PE=3 SV=1)

HSP 1 Score: 1146.3 bits (2964), Expect = 0.0e+00
Identity = 585/630 (92.86%), Postives = 601/630 (95.40%), Query Frame = 0

Query: 12  MGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIF 71
           MGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIF
Sbjct: 1   MGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIF 60

Query: 72  GFLEIIALILITIQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 131
           GFLE+IALILIT+QAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL
Sbjct: 61  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 120

Query: 132 GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAATV 191
           GADQFNQKDPKEAKALGTFFNYMLLSVV+GAAVGVT+IVWVAVNKAWYWGFFISALAA V
Sbjct: 121 GADQFNQKDPKEAKALGTFFNYMLLSVVLGAAVGVTVIVWVAVNKAWYWGFFISALAALV 180

Query: 192 GFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLLLPDTPNELYEISDKLYMDSIH 251
           GFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRL LPDTPNELYEISDK+YMDSIH
Sbjct: 181 GFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLRLPDTPNELYEISDKVYMDSIH 240

Query: 252 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQ 311
           YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQ
Sbjct: 241 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQ 300

Query: 312 LQTFSVEQGNTRIMDKNLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSG 371
           LQTFSVEQGNT IMDK+LGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSG
Sbjct: 301 LQTFSVEQGNTIIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSG 360

Query: 372 ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATKHPDEPMSLFWLAFQYGIFGIADMFTL 431
           ITQLQRVGVGLVLSAIAM VAGLVEVKRRHQA KHPDEP+SLFWLAFQYGIFGIADMFTL
Sbjct: 361 ITQLQRVGVGLVLSAIAMAVAGLVEVKRRHQAIKHPDEPISLFWLAFQYGIFGIADMFTL 420

Query: 432 VGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLSSIFVNVVNKVTKKITPSKKGWVEGLI 491
           VGLLEFFYKEAPVGMRSLSTSFTFLSLA GYYLSSIFVN+VNKVT+ ITPSK+GWVEGLI
Sbjct: 421 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNIVNKVTRNITPSKRGWVEGLI 480

Query: 492 PEDLNHNNLNLFYWFLAILSILNFFHYLYWASWYKYKTEEPILELNGKGGET-------- 551
           PEDLNHNNLNLFYWFLAILS+LNFFHYLYWASWYKYKTEEPI ELN +GGET        
Sbjct: 481 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPIEELNSRGGETTKEPIAEL 540

Query: 552 --------SKEPIAELNKGGKEIKEPIAELNGRGEGETQEPNAELNGGRETKEPIAESNE 611
                   +KEPIAELNK G+EIKEP AELNGRGEGET+EPN ELNGGRETKEPI ESNE
Sbjct: 541 NSKEGETMTKEPIAELNKQGEEIKEPTAELNGRGEGETKEPNVELNGGRETKEPITESNE 600

Query: 612 RGGDDVPILHKEGNGEDAKTHHSEEKEANL 626
           RGGDDVPILHKEGNGEDAKTHHSEEKE+NL
Sbjct: 601 RGGDDVPILHKEGNGEDAKTHHSEEKESNL 630

BLAST of PI0001376 vs. ExPASy TrEMBL
Match: A0A0A0LM59 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G035360 PE=3 SV=1)

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 578/612 (94.44%), Postives = 596/612 (97.39%), Query Frame = 0

Query: 12  MGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIF 71
           MGFVANMTSLVQYFLFVMHFDL+TAANTLTNFMGSAFLLSLLGGFLSDTYINRL TCLIF
Sbjct: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60

Query: 72  GFLEIIALILITIQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 131
           GFLE+IALILIT+QAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL
Sbjct: 61  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 120

Query: 132 GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAATV 191
           GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAA V
Sbjct: 121 GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAALV 180

Query: 192 GFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLLLPDTPNELYEISDKLYMDSIH 251
           GFIIFAIGKPFYRIQVPGQSP+LRVIQVIVVAIKNRRL LPDTPNELYEISDKL+MDS H
Sbjct: 181 GFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLHMDSNH 240

Query: 252 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQ 311
           YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIF+STIIMNTCLAQ
Sbjct: 241 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTIIMNTCLAQ 300

Query: 312 LQTFSVEQGNTRIMDKNLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSG 371
           LQTFSVEQGNTRIMDK+LGHFQFPAPSIPVIPLVFMAFLIPLYEF FVPFARKITHHPSG
Sbjct: 301 LQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARKITHHPSG 360

Query: 372 ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATKHPDEPMSLFWLAFQYGIFGIADMFTL 431
           ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQAT+HPD+ +SLFWLAFQYGIFG+ADMFTL
Sbjct: 361 ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFGVADMFTL 420

Query: 432 VGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLSSIFVNVVNKVTKKITPSKKGWVEGLI 491
           VGLLEFFYKEAPVGMRSLSTSFTFLSLA GYYLSSIFVNVVNKVTK ITPSKKGWVEGLI
Sbjct: 421 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKKGWVEGLI 480

Query: 492 PEDLNHNNLNLFYWFLAILSILNFFHYLYWASWYKYKTEEPILELNGKGGETSKEPIAEL 551
           PEDLNHNNLNLFYWFLAILS+LNFFHYLYWASWYKYKTEEP++ELN KGGET+KEPIAEL
Sbjct: 481 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGETTKEPIAEL 540

Query: 552 NKGGKEIKEPIAE--LNGRGEGETQEPNAELNGGRETKEPIAESNERGGDDVPILHKEGN 611
           NK G+EIKEP     LNGRGEGET+EPNAELNGGRETKEPI ESNERGGDDVPILHKEGN
Sbjct: 541 NKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITESNERGGDDVPILHKEGN 600

Query: 612 GEDAKTHHSEEK 622
           GEDAKTHH+EEK
Sbjct: 601 GEDAKTHHTEEK 612

BLAST of PI0001376 vs. ExPASy TrEMBL
Match: A0A6J1FIK0 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111444459 PE=3 SV=1)

HSP 1 Score: 964.9 bits (2493), Expect = 1.6e-277
Identity = 495/625 (79.20%), Postives = 539/625 (86.24%), Query Frame = 0

Query: 1   MFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDT 60
           MFIF LLTFESMGFVANM SLVQYFL V+HFDL+TAANTLTNF+GSAFLLSLLGGFLSDT
Sbjct: 26  MFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAANTLTNFLGSAFLLSLLGGFLSDT 85

Query: 61  YINRLTTCLIFGFLEIIALILITIQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIG 120
           YINRLTTCLIFG LE++AL+++T+QAYSHDLLP   CPKDCV+GRIAFVFYTSLYLLAIG
Sbjct: 86  YINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKADCPKDCVRGRIAFVFYTSLYLLAIG 145

Query: 121 SGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYW 180
           SGGVRGALPALGADQFNQKDPKEAKAL TFFN +LLSVVIGAAVGVT+IVWVAVNKAWYW
Sbjct: 146 SGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLSVVIGAAVGVTMIVWVAVNKAWYW 205

Query: 181 GFFISALAATVGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLLLPDTPNELYE 240
           GFFISALA  VGFI+FA+GKPFYR+ VPGQSP+LR+IQVIVVA+KNR L LPDTPNELYE
Sbjct: 206 GFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRIIQVIVVAVKNRGLALPDTPNELYE 265

Query: 241 ISDKLYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFI 300
           +SDK YMDSI  KI HTNQLR LDKA+I+PKD+EPQPW VCSVTQVEEVKIITRM+PIFI
Sbjct: 266 LSDKYYMDSIDSKIAHTNQLRCLDKASILPKDVEPQPWKVCSVTQVEEVKIITRMLPIFI 325

Query: 301 STIIMNTCLAQLQTFSVEQGNTRIMDKNLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVP 360
           STIIMNTCLAQLQTFSVEQGNT+IMDK+LGH QFPAPSIPVIPLVFMAFLIPLYEFVFVP
Sbjct: 326 STIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFPAPSIPVIPLVFMAFLIPLYEFVFVP 385

Query: 361 FARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATKHPDEPMSLFWLAFQY 420
           FARKITHHPSGITQLQRVGVGLVLSA++M VAGLVEVKRRHQATKHPDEPMSLFWL+FQY
Sbjct: 386 FARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEVKRRHQATKHPDEPMSLFWLSFQY 445

Query: 421 GIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLSSIFVNVVNKVTKKIT 480
           GIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFT+LSL+FGYYLSSIFVNV+NK+T+K++
Sbjct: 446 GIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLSLSFGYYLSSIFVNVINKITRKVS 505

Query: 481 PSKKGWVEGLIPEDLNHNNLNLFYWFLAILSILNFFHYLYWASWYKYKTEEPILELNGKG 540
           PSKKGWVEGLIPEDLN+NNLNLFYWFLAILS+LNFFHYLYWASWYKYKTEE I++L+  G
Sbjct: 506 PSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFHYLYWASWYKYKTEESIVKLDDGG 565

Query: 541 GETSKEPIAELNKGGKEIKEPIAELNGRGEGETQEPNAELNGGRETKEPIAESNERGGDD 600
           G                           GE E           RE   PI    ER G D
Sbjct: 566 G---------------------------GERE-----------REEGVPILREEERSGGD 611

Query: 601 VPILHKEGNGEDAKTHHSEEKEANL 626
           +   H E   EDA   H EE+ + +
Sbjct: 626 IKCHHSE-EKEDAPIFHEEEERSEI 611

BLAST of PI0001376 vs. NCBI nr
Match: XP_016901445.1 (PREDICTED: protein NRT1/ PTR FAMILY 4.5-like [Cucumis melo])

HSP 1 Score: 1166.8 bits (3017), Expect = 0.0e+00
Identity = 597/641 (93.14%), Postives = 612/641 (95.48%), Query Frame = 0

Query: 1   MFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDT 60
           MFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDT
Sbjct: 40  MFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDT 99

Query: 61  YINRLTTCLIFGFLEIIALILITIQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIG 120
           YINRLTTCLIFGFLE+IALILIT+QAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIG
Sbjct: 100 YINRLTTCLIFGFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIG 159

Query: 121 SGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYW 180
           SGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVV+GAAVGVT+IVWVAVNKAWYW
Sbjct: 160 SGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVLGAAVGVTVIVWVAVNKAWYW 219

Query: 181 GFFISALAATVGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLLLPDTPNELYE 240
           GFFISALAA VGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRL LPDTPNELYE
Sbjct: 220 GFFISALAALVGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLRLPDTPNELYE 279

Query: 241 ISDKLYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFI 300
           ISDK+YMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFI
Sbjct: 280 ISDKVYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFI 339

Query: 301 STIIMNTCLAQLQTFSVEQGNTRIMDKNLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVP 360
           STIIMNTCLAQLQTFSVEQGNT IMDK+LGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVP
Sbjct: 340 STIIMNTCLAQLQTFSVEQGNTIIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVP 399

Query: 361 FARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATKHPDEPMSLFWLAFQY 420
           FARKITHHPSGITQLQRVGVGLVLSAIAM VAGLVEVKRRHQA KHPDEP+SLFWLAFQY
Sbjct: 400 FARKITHHPSGITQLQRVGVGLVLSAIAMAVAGLVEVKRRHQAIKHPDEPISLFWLAFQY 459

Query: 421 GIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLSSIFVNVVNKVTKKIT 480
           GIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLA GYYLSSIFVN+VNKVT+ IT
Sbjct: 460 GIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNIVNKVTRNIT 519

Query: 481 PSKKGWVEGLIPEDLNHNNLNLFYWFLAILSILNFFHYLYWASWYKYKTEEPILELNGKG 540
           PSK+GWVEGLIPEDLNHNNLNLFYWFLAILS+LNFFHYLYWASWYKYKTEEPI ELN KG
Sbjct: 520 PSKRGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPIEELNSKG 579

Query: 541 GET----------------SKEPIAELNKGGKEIKEPIAELNGRGEGETQEPNAELNGGR 600
           GET                +KEPIAELNK G+EIKEP AELNGRGEGET+EPN ELNGGR
Sbjct: 580 GETTKEPIAELNSKEGETMTKEPIAELNKQGEEIKEPTAELNGRGEGETKEPNVELNGGR 639

Query: 601 ETKEPIAESNERGGDDVPILHKEGNGEDAKTHHSEEKEANL 626
           ETKEPI ESNERGGDDVPILHKEGNGEDAKTHHSEEKE+NL
Sbjct: 640 ETKEPITESNERGGDDVPILHKEGNGEDAKTHHSEEKESNL 680

BLAST of PI0001376 vs. NCBI nr
Match: KAA0056094.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucumis melo var. makuwa])

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 596/641 (92.98%), Postives = 612/641 (95.48%), Query Frame = 0

Query: 1   MFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDT 60
           MFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDT
Sbjct: 25  MFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDT 84

Query: 61  YINRLTTCLIFGFLEIIALILITIQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIG 120
           YINRLTTCLIFGFLE+IALILIT+QAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIG
Sbjct: 85  YINRLTTCLIFGFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIG 144

Query: 121 SGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYW 180
           SGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVV+GAAVGVT+IVWVAVNKAWYW
Sbjct: 145 SGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVLGAAVGVTVIVWVAVNKAWYW 204

Query: 181 GFFISALAATVGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLLLPDTPNELYE 240
           GFFISALAA VGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRL LPDTPNELYE
Sbjct: 205 GFFISALAALVGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLRLPDTPNELYE 264

Query: 241 ISDKLYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFI 300
           ISDK+YMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFI
Sbjct: 265 ISDKVYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFI 324

Query: 301 STIIMNTCLAQLQTFSVEQGNTRIMDKNLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVP 360
           STIIMNTCLAQLQTFSVEQGNT IMDK+LGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVP
Sbjct: 325 STIIMNTCLAQLQTFSVEQGNTIIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVP 384

Query: 361 FARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATKHPDEPMSLFWLAFQY 420
           FARKITHHPSGITQLQRVGVGLVLSAIAM VAGLVEVKRRHQA KHPDEP+SLFWLAFQY
Sbjct: 385 FARKITHHPSGITQLQRVGVGLVLSAIAMAVAGLVEVKRRHQAIKHPDEPISLFWLAFQY 444

Query: 421 GIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLSSIFVNVVNKVTKKIT 480
           GIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLA GYYLSSIFVN+VNKVT+ IT
Sbjct: 445 GIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNIVNKVTRNIT 504

Query: 481 PSKKGWVEGLIPEDLNHNNLNLFYWFLAILSILNFFHYLYWASWYKYKTEEPILELNGKG 540
           PSK+GWVEGLIPEDLNHNNLNLFYWFLAILS+LNFFHYLYWASWYKYKTEEPI ELN +G
Sbjct: 505 PSKRGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPIEELNSRG 564

Query: 541 GET----------------SKEPIAELNKGGKEIKEPIAELNGRGEGETQEPNAELNGGR 600
           GET                +KEPIAELNK G+EIKEP AELNGRGEGET+EPN ELNGGR
Sbjct: 565 GETTKEPIAELNSKEGETMTKEPIAELNKQGEEIKEPTAELNGRGEGETKEPNVELNGGR 624

Query: 601 ETKEPIAESNERGGDDVPILHKEGNGEDAKTHHSEEKEANL 626
           ETKEPI ESNERGGDDVPILHKEGNGEDAKTHHSEEKE+NL
Sbjct: 625 ETKEPITESNERGGDDVPILHKEGNGEDAKTHHSEEKESNL 665

BLAST of PI0001376 vs. NCBI nr
Match: TYJ96424.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucumis melo var. makuwa])

HSP 1 Score: 1146.3 bits (2964), Expect = 0.0e+00
Identity = 585/630 (92.86%), Postives = 601/630 (95.40%), Query Frame = 0

Query: 12  MGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIF 71
           MGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIF
Sbjct: 1   MGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIF 60

Query: 72  GFLEIIALILITIQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 131
           GFLE+IALILIT+QAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL
Sbjct: 61  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 120

Query: 132 GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAATV 191
           GADQFNQKDPKEAKALGTFFNYMLLSVV+GAAVGVT+IVWVAVNKAWYWGFFISALAA V
Sbjct: 121 GADQFNQKDPKEAKALGTFFNYMLLSVVLGAAVGVTVIVWVAVNKAWYWGFFISALAALV 180

Query: 192 GFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLLLPDTPNELYEISDKLYMDSIH 251
           GFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRL LPDTPNELYEISDK+YMDSIH
Sbjct: 181 GFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLRLPDTPNELYEISDKVYMDSIH 240

Query: 252 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQ 311
           YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQ
Sbjct: 241 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQ 300

Query: 312 LQTFSVEQGNTRIMDKNLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSG 371
           LQTFSVEQGNT IMDK+LGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSG
Sbjct: 301 LQTFSVEQGNTIIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSG 360

Query: 372 ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATKHPDEPMSLFWLAFQYGIFGIADMFTL 431
           ITQLQRVGVGLVLSAIAM VAGLVEVKRRHQA KHPDEP+SLFWLAFQYGIFGIADMFTL
Sbjct: 361 ITQLQRVGVGLVLSAIAMAVAGLVEVKRRHQAIKHPDEPISLFWLAFQYGIFGIADMFTL 420

Query: 432 VGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLSSIFVNVVNKVTKKITPSKKGWVEGLI 491
           VGLLEFFYKEAPVGMRSLSTSFTFLSLA GYYLSSIFVN+VNKVT+ ITPSK+GWVEGLI
Sbjct: 421 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNIVNKVTRNITPSKRGWVEGLI 480

Query: 492 PEDLNHNNLNLFYWFLAILSILNFFHYLYWASWYKYKTEEPILELNGKGGET-------- 551
           PEDLNHNNLNLFYWFLAILS+LNFFHYLYWASWYKYKTEEPI ELN +GGET        
Sbjct: 481 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPIEELNSRGGETTKEPIAEL 540

Query: 552 --------SKEPIAELNKGGKEIKEPIAELNGRGEGETQEPNAELNGGRETKEPIAESNE 611
                   +KEPIAELNK G+EIKEP AELNGRGEGET+EPN ELNGGRETKEPI ESNE
Sbjct: 541 NSKEGETMTKEPIAELNKQGEEIKEPTAELNGRGEGETKEPNVELNGGRETKEPITESNE 600

Query: 612 RGGDDVPILHKEGNGEDAKTHHSEEKEANL 626
           RGGDDVPILHKEGNGEDAKTHHSEEKE+NL
Sbjct: 601 RGGDDVPILHKEGNGEDAKTHHSEEKESNL 630

BLAST of PI0001376 vs. NCBI nr
Match: KGN61036.1 (hypothetical protein Csa_021307 [Cucumis sativus])

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 578/612 (94.44%), Postives = 596/612 (97.39%), Query Frame = 0

Query: 12  MGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIF 71
           MGFVANMTSLVQYFLFVMHFDL+TAANTLTNFMGSAFLLSLLGGFLSDTYINRL TCLIF
Sbjct: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60

Query: 72  GFLEIIALILITIQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 131
           GFLE+IALILIT+QAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL
Sbjct: 61  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 120

Query: 132 GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAATV 191
           GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAA V
Sbjct: 121 GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAALV 180

Query: 192 GFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLLLPDTPNELYEISDKLYMDSIH 251
           GFIIFAIGKPFYRIQVPGQSP+LRVIQVIVVAIKNRRL LPDTPNELYEISDKL+MDS H
Sbjct: 181 GFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLHMDSNH 240

Query: 252 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQ 311
           YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIF+STIIMNTCLAQ
Sbjct: 241 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTIIMNTCLAQ 300

Query: 312 LQTFSVEQGNTRIMDKNLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSG 371
           LQTFSVEQGNTRIMDK+LGHFQFPAPSIPVIPLVFMAFLIPLYEF FVPFARKITHHPSG
Sbjct: 301 LQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARKITHHPSG 360

Query: 372 ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATKHPDEPMSLFWLAFQYGIFGIADMFTL 431
           ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQAT+HPD+ +SLFWLAFQYGIFG+ADMFTL
Sbjct: 361 ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFGVADMFTL 420

Query: 432 VGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLSSIFVNVVNKVTKKITPSKKGWVEGLI 491
           VGLLEFFYKEAPVGMRSLSTSFTFLSLA GYYLSSIFVNVVNKVTK ITPSKKGWVEGLI
Sbjct: 421 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKKGWVEGLI 480

Query: 492 PEDLNHNNLNLFYWFLAILSILNFFHYLYWASWYKYKTEEPILELNGKGGETSKEPIAEL 551
           PEDLNHNNLNLFYWFLAILS+LNFFHYLYWASWYKYKTEEP++ELN KGGET+KEPIAEL
Sbjct: 481 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGETTKEPIAEL 540

Query: 552 NKGGKEIKEPIAE--LNGRGEGETQEPNAELNGGRETKEPIAESNERGGDDVPILHKEGN 611
           NK G+EIKEP     LNGRGEGET+EPNAELNGGRETKEPI ESNERGGDDVPILHKEGN
Sbjct: 541 NKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITESNERGGDDVPILHKEGN 600

Query: 612 GEDAKTHHSEEK 622
           GEDAKTHH+EEK
Sbjct: 601 GEDAKTHHTEEK 612

BLAST of PI0001376 vs. NCBI nr
Match: XP_031736687.1 (protein NRT1/ PTR FAMILY 4.5 [Cucumis sativus])

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 572/619 (92.41%), Postives = 590/619 (95.32%), Query Frame = 0

Query: 18  MTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIFGFLEII 77
           MTSLVQYFLFVMHFDL+TAANTLTNFMGSAFLLSLLGGFLSDTYINRL TCLIFGFLE+I
Sbjct: 1   MTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIFGFLEVI 60

Query: 78  -------------ALILITIQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGV 137
                        ALILIT+QAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGV
Sbjct: 61  FHIYCIKRYEYTQALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGV 120

Query: 138 RGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFI 197
           RGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFI
Sbjct: 121 RGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFI 180

Query: 198 SALAATVGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLLLPDTPNELYEISDK 257
           SALAA VGFIIFAIGKPFYRIQVPGQSP+LRVIQVIVVAIKNRRL LPDTPNELYEISDK
Sbjct: 181 SALAALVGFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDK 240

Query: 258 LYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFISTII 317
           L+MDS HYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIF+STII
Sbjct: 241 LHMDSNHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTII 300

Query: 318 MNTCLAQLQTFSVEQGNTRIMDKNLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARK 377
           MNTCLAQLQTFSVEQGNTRIMDK+LGHFQFPAPSIPVIPLVFMAFLIPLYEF FVPFARK
Sbjct: 301 MNTCLAQLQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARK 360

Query: 378 ITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATKHPDEPMSLFWLAFQYGIFG 437
           ITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQAT+HPD+ +SLFWLAFQYGIFG
Sbjct: 361 ITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFG 420

Query: 438 IADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLSSIFVNVVNKVTKKITPSKK 497
           +ADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLA GYYLSSIFVNVVNKVTK ITPSKK
Sbjct: 421 VADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKK 480

Query: 498 GWVEGLIPEDLNHNNLNLFYWFLAILSILNFFHYLYWASWYKYKTEEPILELNGKGGETS 557
           GWVEGLIPEDLNHNNLNLFYWFLAILS+LNFFHYLYWASWYKYKTEEP++ELN KGGET+
Sbjct: 481 GWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGETT 540

Query: 558 KEPIAELNKGGKEIKEPIAE--LNGRGEGETQEPNAELNGGRETKEPIAESNERGGDDVP 617
           KEPIAELNK G+EIKEP     LNGRGEGET+EPNAELNGGRETKEPI ESNERGGDDVP
Sbjct: 541 KEPIAELNKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITESNERGGDDVP 600

Query: 618 ILHKEGNGEDAKTHHSEEK 622
           ILHKEGNGEDAKTHH+EEK
Sbjct: 601 ILHKEGNGEDAKTHHTEEK 619

BLAST of PI0001376 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 405.6 bits (1041), Expect = 7.2e-113
Identity = 234/537 (43.58%), Postives = 334/537 (62.20%), Query Frame = 0

Query: 1   MFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDT 60
           +F+ GL  FE MG  A   +L+ Y +  MHF L  AAN +TNF+G+ F+ +LLGG+LSD 
Sbjct: 49  LFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTIFIFALLGGYLSDA 108

Query: 61  YINRLTTCLIFGFLEIIALILITIQAYSHDLLP---SPLCPKDC--VKGRIAFVFYTSLY 120
           ++    T +IFGF+E+   IL+++QA+   L P   +PL  + C   KG  A +F+ +LY
Sbjct: 109 FLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNPLIDQTCEEAKGFKAMIFFMALY 168

Query: 121 LLAIGSGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVN 180
           L+A+GSG V+  + A GADQF+Q  PK++K L ++FN    +  +G  + +TL+VWV  +
Sbjct: 169 LVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTH 228

Query: 181 KAWYWGFFISALAATVGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLLLPDTP 240
                GF +SA A T+G I    G  ++R + P +S    +  VIV AI  R+L  P  P
Sbjct: 229 SGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKLASPSDP 288

Query: 241 NEL---YEISDKLYMDSIHYKIVHTNQLRFLDKAAIVPKD--IEPQPWNVCSVTQVEEVK 300
             L   + +++ +   S    + HT + RFLDKA I  +D   +  PW +C+VTQVE+VK
Sbjct: 289 RMLHGDHHVANDVVPSS---TLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQVEQVK 348

Query: 301 IITRMVPIFISTIIMNTCLAQLQTFSVEQG---NTRIMDKNLGHFQFPAPSIPVIPLVFM 360
            +  +VPIF STI+ NT LAQLQTFSV+QG   NTR+ +     F  P  S+  IP + +
Sbjct: 349 TLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNTRLSNS----FHIPPASLQAIPYIML 408

Query: 361 AFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATKHP 420
            FL+PLY+   VPFARK+T H SGI  L R+G+GL LS  +M  A ++E KRR  +    
Sbjct: 409 IFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVL-D 468

Query: 421 DEPMSLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLSSI 480
              +S+FW+  Q+ IFGI++MFT VGL+EFFYK++  GM S   + T+ S +FG+Y SS+
Sbjct: 469 GRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSV 528

Query: 481 FVNVVNKVTKKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSILNFFHYLYWASW 525
            V+VVNK+T     S KGW   L   DLN + L+LFYW LA+LS+LNF  YL+W+ W
Sbjct: 529 LVSVVNKITSTSVDS-KGW---LGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573

BLAST of PI0001376 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 395.6 bits (1015), Expect = 7.5e-110
Identity = 227/542 (41.88%), Postives = 330/542 (60.89%), Query Frame = 0

Query: 2   FIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTY 61
           F+  +   E++ F+AN ++LV Y    MH  L  +++ +T FM +AFLL+LLGGFL+D +
Sbjct: 32  FVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAF 91

Query: 62  INRLTTCLIFGFLEIIALILITIQAYSHDLLPSPLCPK-----DCVKGRIAFVFYTSLYL 121
            +     LI   +E + LIL+TIQA    L+P P         + V G  A   +  LYL
Sbjct: 92  FSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSAALRCEVVGGSKAAFLFVGLYL 151

Query: 122 LAIGSGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNK 181
           +++G GG++G+LP+ GA+QF++  PK  K   TFFNY +  +  GA V VT +VW+  NK
Sbjct: 152 VSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNK 211

Query: 182 AWYWGFFISALAATVGFIIFAIGKPFYRIQVPGQSPILRVIQVI----VVAIKNRRLLLP 241
            W WGF +S ++  +  ++F +G  FY+ ++P  SP+  + +V+    +V+  ++     
Sbjct: 212 GWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNH 271

Query: 242 DTPNELY-EISDKLYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKI 301
            T  E+  E  +K    S+      TN L  L+KA    +      W  C+V QVE+VKI
Sbjct: 272 FTSREVQSEHEEKTPSQSL------TNSLTCLNKAI---EGKTHHIWLECTVQQVEDVKI 331

Query: 302 ITRMVPIFISTIIMNTCLAQLQTFSVEQGNTRIMDKNLGHFQFPAPSIPVIPLVFMAFLI 361
           + +M+PIF  TI++N CLAQL T+SV Q  T  M++ + +F  P+ S+PV P+VFM  L 
Sbjct: 332 VLKMLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFPVVFMLILA 391

Query: 362 PLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATK------ 421
           P Y+ + +PFARK+T    GIT LQR+GVGLVLS +AM VA LVE+KR+  A +      
Sbjct: 392 PTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDS 451

Query: 422 HPDEPMSLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLS 481
               P++  W+A QY   G AD+FTL GLLEFF+ EAP  MRSL+TS ++ SLA GYYLS
Sbjct: 452 EETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLS 511

Query: 482 SIFVNVVNKVTKKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSILNFFHYLYWASWY 528
           S+ V +VN+VTK  +  +  W    + E LN N L+LFYW + +LS++NF HYL+WA  Y
Sbjct: 512 SVMVPIVNRVTK--SAGQSPW----LGEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRY 556

BLAST of PI0001376 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 395.6 bits (1015), Expect = 7.5e-110
Identity = 227/542 (41.88%), Postives = 330/542 (60.89%), Query Frame = 0

Query: 2   FIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTY 61
           F+  +   E++ F+AN ++LV Y    MH  L  +++ +T FM +AFLL+LLGGFL+D +
Sbjct: 36  FVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAF 95

Query: 62  INRLTTCLIFGFLEIIALILITIQAYSHDLLPSPLCPK-----DCVKGRIAFVFYTSLYL 121
            +     LI   +E + LIL+TIQA    L+P P         + V G  A   +  LYL
Sbjct: 96  FSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSAALRCEVVGGSKAAFLFVGLYL 155

Query: 122 LAIGSGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNK 181
           +++G GG++G+LP+ GA+QF++  PK  K   TFFNY +  +  GA V VT +VW+  NK
Sbjct: 156 VSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNK 215

Query: 182 AWYWGFFISALAATVGFIIFAIGKPFYRIQVPGQSPILRVIQVI----VVAIKNRRLLLP 241
            W WGF +S ++  +  ++F +G  FY+ ++P  SP+  + +V+    +V+  ++     
Sbjct: 216 GWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNH 275

Query: 242 DTPNELY-EISDKLYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKI 301
            T  E+  E  +K    S+      TN L  L+KA    +      W  C+V QVE+VKI
Sbjct: 276 FTSREVQSEHEEKTPSQSL------TNSLTCLNKAI---EGKTHHIWLECTVQQVEDVKI 335

Query: 302 ITRMVPIFISTIIMNTCLAQLQTFSVEQGNTRIMDKNLGHFQFPAPSIPVIPLVFMAFLI 361
           + +M+PIF  TI++N CLAQL T+SV Q  T  M++ + +F  P+ S+PV P+VFM  L 
Sbjct: 336 VLKMLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFPVVFMLILA 395

Query: 362 PLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATK------ 421
           P Y+ + +PFARK+T    GIT LQR+GVGLVLS +AM VA LVE+KR+  A +      
Sbjct: 396 PTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDS 455

Query: 422 HPDEPMSLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLS 481
               P++  W+A QY   G AD+FTL GLLEFF+ EAP  MRSL+TS ++ SLA GYYLS
Sbjct: 456 EETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLS 515

Query: 482 SIFVNVVNKVTKKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSILNFFHYLYWASWY 528
           S+ V +VN+VTK  +  +  W    + E LN N L+LFYW + +LS++NF HYL+WA  Y
Sbjct: 516 SVMVPIVNRVTK--SAGQSPW----LGEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRY 560

BLAST of PI0001376 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 384.4 bits (986), Expect = 1.7e-106
Identity = 225/560 (40.18%), Postives = 332/560 (59.29%), Query Frame = 0

Query: 2   FIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTY 61
           F+  +   E++ ++AN ++LV Y    MH     +AN +TNFMG+AFLL+LLGGFLSD +
Sbjct: 34  FVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLGGFLSDAF 93

Query: 62  INRLTTCLIFGFLEIIALILITIQAYSHDLLP----SPLCPKDCVKGRIAFVFYTSLYLL 121
            +     LI   +E + LI++TIQA +  L+P    SP C +  V G  A + +  LYL+
Sbjct: 94  FSTFQIFLISASIEFLGLIILTIQARTPSLMPPSCDSPTCEE--VSGSKAAMLFVGLYLV 153

Query: 122 AIGSGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKA 181
           A+G GG++G+L + GA+QF++  PK  K   TFFNY +  +  GA V VT +VW+  NK 
Sbjct: 154 ALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKG 213

Query: 182 WYWGFFISALAATVGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVA---------IKNRR 241
           W WGF +S +A  V  +IF  G  FYR ++P  SP+  +++V++ A           N  
Sbjct: 214 WEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSGSSSNAV 273

Query: 242 LLLPDTPN------------ELYEISDKLYMDSIHYKIVHTNQLRFLDKAAIVPKDIEP- 301
             +  +P+               E+      +++  +   TN L+ L+ AA    D +P 
Sbjct: 274 ASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAA----DEKPV 333

Query: 302 QPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQLQTFSVEQGNTRIMDKNLGHFQFP 361
                C+V QVE+VKI+ +M+PIF  TI++N CLAQL TFSV+Q  +  M+  +G  + P
Sbjct: 334 HRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAAS--MNTKIGSLKIP 393

Query: 362 APSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLV 421
             S+P+ P+VF+  L P+Y+ + +PFARK T   +G+T LQR+GVGLVLS +AM VA LV
Sbjct: 394 PASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALV 453

Query: 422 EVKRRHQA------TKHPDEPMSLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSL 481
           E+KR+  A            P++  W+A QY   G AD+FTL GLLE+F+ EAP  MRSL
Sbjct: 454 EIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSL 513

Query: 482 STSFTFLSLAFGYYLSSIFVNVVNKVTKKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAI 530
           +TS ++ SLA GYYLSS+ V++VN +T   +     W+ G   + +N   L+ FYW + +
Sbjct: 514 ATSLSWASLAMGYYLSSVIVSIVNSITG--SSGNTPWLRG---KSINRYKLDYFYWLMCV 573

BLAST of PI0001376 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 384.0 bits (985), Expect = 2.3e-106
Identity = 229/537 (42.64%), Postives = 326/537 (60.71%), Query Frame = 0

Query: 1   MFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDT 60
           +F+ G   FE M   A   +L+ Y    MHF L  +AN +TNF+G+ FLLSLLGGFLSD+
Sbjct: 46  LFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSLLGGFLSDS 105

Query: 61  YINRLTTCLIFGFLEIIALILITIQAYSHDLLPSPLCPKD----CVK--GRIAFVFYTSL 120
           Y+    T L+FG +EI   IL+++QA+  +L P     K     CV+  G  A   YT+L
Sbjct: 106 YLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYKAATLYTAL 165

Query: 121 YLLAIGSGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAV 180
            L+A+GSG ++  + + GA+QF +KD    + L +FFN    +  +G  + +TL+VWV  
Sbjct: 166 CLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIALTLLVWVQT 225

Query: 181 NKAWYWGFFISALAATVGFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLLLPDT 240
           +     GF +SA     G I    G  FYR + P  S    + QV V AI  R+ + P  
Sbjct: 226 HSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITKRKQICPSN 285

Query: 241 PNELYEISDKLYMDSIHYK-IVHTNQLRFLDKAAIVPK-DIEPQPWNVCSVTQVEEVKII 300
           PN +++ S     D +  K ++H+N+ RFLDKA I  +      PW +C++ QV +VKI+
Sbjct: 286 PNMVHQPS----TDLVRVKPLLHSNKFRFLDKACIKTQGKAMESPWRLCTIEQVHQVKIL 345

Query: 301 TRMVPIFISTIIMNTCLAQLQTFSVEQG---NTRIMDKNLGHFQFPAPSIPVIPLVFMAF 360
             ++PIF  TII NT LAQLQTFSV+QG   NT I       FQ P  S+  IP + + F
Sbjct: 346 LSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKT----FQIPPASLQAIPYIILIF 405

Query: 361 LIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATKHPDE 420
            +PLYE  FVP ARK+T + SGI+ LQR+G GL L+  +M  A LVE KRR ++    + 
Sbjct: 406 FVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRR-ESFLEQNV 465

Query: 421 PMSLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLAFGYYLSSIFV 480
            +S+FW+A Q+ IFG+++MFT VGL+EFFYK++   M+S  T+ T+ S +FG+YLSS+ V
Sbjct: 466 MLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSVLV 525

Query: 481 NVVNKVTKKI-TPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSILNFFHYLYWASWY 526
           + VN+VT    + +K+GW   L   DLN + L+ FYW LA LS +NFF+YL+W+ WY
Sbjct: 526 STVNRVTSSNGSGTKEGW---LGDNDLNKDRLDHFYWLLASLSFINFFNYLFWSRWY 567