PI0000875 (gene) Melon (PI 482460) v1

BLAST of PI0000875 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 3.7e-12
Identity = 119/550 (21.64%), Postives = 220/550 (40.00%), Query Frame = 0

Query: 24  RWLVLFFSLLLMAVAGATYMFSLYSNDIKLIW--------------DLGANVGILSGLIN 83
           +W VL  ++ + A  G  + FS YS+ +K +               DLG   G  SG+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 84  EVTPAWVVLSIGAVMNFFGYFMIWLTITRRTSAPKVWQMCLYICIGAN-SQSFANTGSMV 143
              P  VVL   A M F GY + WL IT   + P  + +    C+ A  S  + NT   +
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP--YSLVFLCCLLAGLSICWFNTACFI 162

Query: 144 TCVKNFPESRGAVLGILKGYIGLSGAIITQLFHAFYGADTKSLILFIGWLPTAISFASL- 203
            C+++FP +R   L +   + G+S A+ +  F+A   + +   +L    +P  +SFA+L 
Sbjct: 163 LCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALY 222

Query: 204 PIIRIMKVIRQPN-ELKVFYNFLYISLSLAGFL--MLLIILESKTEFTQTNTVAAP---- 263
           P++    +   P+ + +   + ++  L++   +    L++  S T   + N + A     
Sbjct: 223 PVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVVLLV 282

Query: 264 ----------------PSSSSFSSSHSSSSSSKNTISGNSKPLSLNLQLPLQEQKEPSCW 323
                           P  ++  +  SS     N     ++  S++ +   +        
Sbjct: 283 FPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAKEG 342

Query: 324 TTIFSPPERGEDFTILQAFFSVDMLILFTSAACGIGGTLTAIDNLGQI-------GITFV 383
            T+      G++ +       ++  + + +  CG    L   +NLGQI         T V
Sbjct: 343 NTV----RLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNSTTLV 402

Query: 384 TLVSVWNYLGRVACGFFSEMVLIKYKFPLMAGFLSEHLLIKYKFPRPLMLTIVLLLSCIA 443
           T+ S +++ GR                  +     + +  +++  R     I LL + IA
Sbjct: 403 TIYSSFSFFGR------------------LLSAAPDFMHKRFRLTRTGWFAIALLPTPIA 462

Query: 444 HLLIAFNPS--GGLYIASVLTGFCYGAQWPLLFAIVSEIFGLKYYATLYNFGSVASPVGL 503
             L+A + S    L  A+ L G   G  +    +I S++FG       +N      P+G 
Sbjct: 463 FFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIG- 522

Query: 504 YVLNVNVAGYLYDKEAKKQLAMTGRIRKTGEELICNGTVCFKLSFVIITAVSLFGALVSL 526
            +L   +A  +Y+  A   +          + ++C G  C+  +FV    +S+ G + SL
Sbjct: 523 SLLYGYIAASIYEANASPDITPI-----VSDSIVCIGRDCYFKTFVFWGCLSILGVVSSL 562

BLAST of PI0000875 vs. ExPASy TrEMBL
Match: A0A6J1GZI8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111458291 PE=4 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 2.7e-199
Identity = 392/612 (64.05%), Postives = 455/612 (74.35%), Query Frame = 0

Query: 1   MLAGGRILGCSHIRTIVVHIITGRWLVLFFSLLLMAVAGATYMFSLYSNDIKLIW----- 60
           M+AGG I GCS I +I VH+ITGRW V+F SLL+MA AGATYMFSLYS+DIK +      
Sbjct: 1   MVAGGGISGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQT 60

Query: 61  ---------DLGANVGILSGLINEVTPAWVVLSIGAVMNFFGYFMIWLTITRRTSAPKVW 120
                    DLGANVG+LSGLINEVTP WVVLSIGAVMNFFGYFMIWL +TR+ +APKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTRKIAAPKVW 120

Query: 121 QMCLYICIGANSQSFANTGSMVTCVKNFPESRGAVLGILKGYIGLSGAIITQLFHAFYGA 180
           QMCLYICIGANSQSFANTGS+VTCVKNFPESRG VLGILKGY+GLSGAIITQLFHAFYG 
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGG 180

Query: 181 DTKSLILFIGWLPTAISFASLPIIRIMKVIRQPNELKVFYNFLYISLSLAGFLMLLIILE 240
           DTKSLIL IGWLP AISFA L  IRIMKVIRQPNELKVFYNFLYISL+LAGFLML+II+E
Sbjct: 181 DTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKTEFTQTN-------------TVAAPPSSSSFSSSHSSSSSSKNTISGNSKPLSL-NLQ 300
           SKTEF+Q+                 A      ++  +  ++++   I   + P+ +   +
Sbjct: 241 SKTEFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAIRSPNHPVQIVTEK 300

Query: 301 LPLQE----------QKEPSCWTTIFSPPERGEDFTILQAFFSVDMLILFTSAACGIGGT 360
           LP  E          QKEPSCWTTIF+PPERGEDFTILQA FSVDMLILF +A CG+GGT
Sbjct: 301 LPKTEHQKHIPKTEHQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLAAICGVGGT 360

Query: 361 LTAIDNLGQIGI----------TFVTLVSVWNYLGRVACGFFSEMVLIKYKFPLMAGFLS 420
           LTAIDNLGQIG+          TFVTLVS+WNYLGRV  GF SE+V              
Sbjct: 361 LTAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIV-------------- 420

Query: 421 EHLLIKYKFPRPLMLTIVLLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVS 480
              L KYKFPRPLML++ LL+SC+ HL+IAF+   GLY+AS++ GFC+GAQWPL+FAI+S
Sbjct: 421 ---LTKYKFPRPLMLSMTLLVSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIIS 480

Query: 481 EIFGLKYYATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMTGRIRKTGEELICNG 540
           E+FGLKYY+TLYNFGSVASP+GLYVLNV VAG  YD+EA+KQL   G +RKTGEEL C G
Sbjct: 481 ELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFG 540

Query: 541 TVCFKLSFVIITAVSLFGALVSLVLVLRTKKFYKSDIYKKFREAEEAAAVEEEEDDGRVV 565
             CFKLSF++IT V+L G  VSL+LV+RT+ FYKSDIYKKFR+  E     E   +G VV
Sbjct: 541 GECFKLSFLVITGVTLLGMFVSLILVIRTRSFYKSDIYKKFRDEAET----EMAGNGAVV 589

BLAST of PI0000875 vs. ExPASy TrEMBL
Match: A0A5D3CFW3 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002400 PE=4 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 3.6e-199
Identity = 391/604 (64.74%), Postives = 447/604 (74.01%), Query Frame = 0

Query: 1   MLAGGRILGCSHIRTIVVHIITGRWLVLFFSLLLMAVAGATYMFSLYSNDIKLIW----- 60
           M+AGG I GCS I +I VH+ITGRW V+F SLL+MA AGATYMF LYS+DIK +      
Sbjct: 1   MVAGGGISGCSDIGSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60

Query: 61  ---------DLGANVGILSGLINEVTPAWVVLSIGAVMNFFGYFMIWLTITRRTSAPKVW 120
                    DLGANVG+LSGLINEVTP WVVLSIGAV+NFFGYFMIWL +TRR S PKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVW 120

Query: 121 QMCLYICIGANSQSFANTGSMVTCVKNFPESRGAVLGILKGYIGLSGAIITQLFHAFYGA 180
           QMCLYICIGANSQSFANTGS+VTCVKNFPESRG VLGILKGY+GLSGAIITQLFHAFYG 
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGN 180

Query: 181 DTKSLILFIGWLPTAISFASLPIIRIMKVIRQPNELKVFYNFLYISLSLAGFLMLLIILE 240
           DTKSLIL IGWLP AISFASL  IRIMKVIRQPNELKVFYNFLYISL+LAGFLML+II+E
Sbjct: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKTEFTQTN----------TVAAPPSSSSFSSSHSSSSSSKNTISGNSKPLSLNLQLPLQ 300
           SK EF Q             +  P S       +     +    S N     +  +LP  
Sbjct: 241 SKKEFNQNEYGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVKSPNPSVQIVTEKLPKT 300

Query: 301 ------EQKEPSCWTTIFSPPERGEDFTILQAFFSVDMLILFTSAACGIGGTLTAIDNLG 360
                 EQKEPSCWTTIF+PP+RGEDFTILQA FS DMLILF +A CG+GGTLTAIDNLG
Sbjct: 301 EHPKQLEQKEPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIAAICGVGGTLTAIDNLG 360

Query: 361 QIGI----------TFVTLVSVWNYLGRVACGFFSEMVLIKYKFPLMAGFLSEHLLIKYK 420
           QIG+          TFVTLVS+WNYLGRVA GF SE+V                 L KYK
Sbjct: 361 QIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFISEIV-----------------LTKYK 420

Query: 421 FPRPLMLTIVLLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEIFGLKYY 480
           FPRPL+L++ LLLSC+ HL+IAF+   GLY+AS++ GFC+GAQWPL+FAI+SE+FGLKYY
Sbjct: 421 FPRPLILSLTLLLSCVGHLIIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYY 480

Query: 481 ATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMTGRIRKTGEELICNGTVCFKLSF 540
           +TLYNFGSVASP+GLY+LNV VAG  YD+EA+KQL+  G IRK GEEL C G  CFKLSF
Sbjct: 481 STLYNFGSVASPIGLYILNVKVAGNFYDREAEKQLSAKGIIRKAGEELKCFGGECFKLSF 540

Query: 541 VIITAVSLFGALVSLVLVLRTKKFYKSDIYKKFREAEEAAAVEEEEDDGRVVRNGGGLME 565
           ++ITAV+L G LVSLVLV+RT+ FYKSDIYKKFR+       E +E  G  V    G+ E
Sbjct: 541 IVITAVTLLGMLVSLVLVIRTRSFYKSDIYKKFRD-----EAETKEVAGNAVIEVAGVPE 582

BLAST of PI0000875 vs. ExPASy TrEMBL
Match: A0A1S3BG09 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 PE=4 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 3.6e-199
Identity = 391/604 (64.74%), Postives = 447/604 (74.01%), Query Frame = 0

Query: 1   MLAGGRILGCSHIRTIVVHIITGRWLVLFFSLLLMAVAGATYMFSLYSNDIKLIW----- 60
           M+AGG I GCS I +I VH+ITGRW V+F SLL+MA AGATYMF LYS+DIK +      
Sbjct: 1   MVAGGGISGCSDIGSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQT 60

Query: 61  ---------DLGANVGILSGLINEVTPAWVVLSIGAVMNFFGYFMIWLTITRRTSAPKVW 120
                    DLGANVG+LSGLINEVTP WVVLSIGAV+NFFGYFMIWL +TRR S PKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVW 120

Query: 121 QMCLYICIGANSQSFANTGSMVTCVKNFPESRGAVLGILKGYIGLSGAIITQLFHAFYGA 180
           QMCLYICIGANSQSFANTGS+VTCVKNFPESRG VLGILKGY+GLSGAIITQLFHAFYG 
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGN 180

Query: 181 DTKSLILFIGWLPTAISFASLPIIRIMKVIRQPNELKVFYNFLYISLSLAGFLMLLIILE 240
           DTKSLIL IGWLP AISFASL  IRIMKVIRQPNELKVFYNFLYISL+LAGFLML+II+E
Sbjct: 181 DTKSLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKTEFTQTN----------TVAAPPSSSSFSSSHSSSSSSKNTISGNSKPLSLNLQLPLQ 300
           SK EF Q             +  P S       +     +    S N     +  +LP  
Sbjct: 241 SKKEFNQNEYGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVKSPNPSVQIVTEKLPKT 300

Query: 301 ------EQKEPSCWTTIFSPPERGEDFTILQAFFSVDMLILFTSAACGIGGTLTAIDNLG 360
                 EQKEPSCWTTIF+PP+RGEDFTILQA FS DMLILF +A CG+GGTLTAIDNLG
Sbjct: 301 EHPKQLEQKEPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIAAICGVGGTLTAIDNLG 360

Query: 361 QIGI----------TFVTLVSVWNYLGRVACGFFSEMVLIKYKFPLMAGFLSEHLLIKYK 420
           QIG+          TFVTLVS+WNYLGRVA GF SE+V                 L KYK
Sbjct: 361 QIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFISEIV-----------------LTKYK 420

Query: 421 FPRPLMLTIVLLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEIFGLKYY 480
           FPRPL+L++ LLLSC+ HL+IAF+   GLY+AS++ GFC+GAQWPL+FAI+SE+FGLKYY
Sbjct: 421 FPRPLILSLTLLLSCVGHLIIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYY 480

Query: 481 ATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMTGRIRKTGEELICNGTVCFKLSF 540
           +TLYNFGSVASP+GLY+LNV VAG  YD+EA+KQL+  G IRK GEEL C G  CFKLSF
Sbjct: 481 STLYNFGSVASPIGLYILNVKVAGNFYDREAEKQLSAKGIIRKAGEELKCFGGECFKLSF 540

Query: 541 VIITAVSLFGALVSLVLVLRTKKFYKSDIYKKFREAEEAAAVEEEEDDGRVVRNGGGLME 565
           ++ITAV+L G LVSLVLV+RT+ FYKSDIYKKFR+       E +E  G  V    G+ E
Sbjct: 541 IVITAVTLLGMLVSLVLVIRTRSFYKSDIYKKFRD-----EAETKEVAGNAVIEVAGVPE 582

BLAST of PI0000875 vs. ExPASy TrEMBL
Match: A0A6J1FZF2 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111449326 PE=4 SV=1)

HSP 1 Score: 704.1 bits (1816), Expect = 4.6e-199
Identity = 390/610 (63.93%), Postives = 444/610 (72.79%), Query Frame = 0

Query: 1   MLAGGR-----ILGCSHIRTIVVHIITGRWLVLFFSLLLMAVAGATYMFSLYSNDIKLIW 60
           M+A G+     I G S+IRTI VHIITGRW V+F SLL+MA AGATYMFSLYSNDIK + 
Sbjct: 1   MVAAGQGGDRAISGSSNIRTIAVHIITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL 60

Query: 61  --------------DLGANVGILSGLINEVTPAWVVLSIGAVMNFFGYFMIWLTITRRTS 120
                         DLGANVG+LSGLINEVTP WVVLSIGAV+NFFGYFMIWL +T+R S
Sbjct: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS 120

Query: 121 APKVWQMCLYICIGANSQSFANTGSMVTCVKNFPESRGAVLGILKGYIGLSGAIITQLFH 180
            PKVWQMCLYIC+GANSQSF NTGS+VTCVKNFPESRG VLGILKGY+GLSGAIITQLFH
Sbjct: 121 TPKVWQMCLYICVGANSQSFVNTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFH 180

Query: 181 AFYGADTKSLILFIGWLPTAISFASLPIIRIMKVIRQPNELKVFYNFLYISLSLAGFLML 240
           AFYG+DTKSLIL IGWLP AISFA L  IRIMKVIRQPNELKVFYNFLYISL+LAGFLML
Sbjct: 181 AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML 240

Query: 241 LIILESKTEFTQTN------------------TVAAPPSSSSFSSSHSSSSSSKNTISGN 300
           +II+ESK  F+Q                     +    + S   S+  +  +    ++  
Sbjct: 241 MIIIESKLIFSQAEFGGSAAVVLLLLFLPLAVVIVEEYNLSKLKSAAINPPNPLQILTEK 300

Query: 301 SKPLSLNLQLPLQEQKEPSCWTTIFSPPERGEDFTILQAFFSVDMLILFTSAACGIGGTL 360
             P     Q   ++QK+PSCWTTIFSPPERGEDFTILQA FS+DM ILF SA CG+GGTL
Sbjct: 301 PNPPKTEPQKQTRQQKQPSCWTTIFSPPERGEDFTILQALFSLDMAILFFSAICGVGGTL 360

Query: 361 TAIDNLGQIGI----------TFVTLVSVWNYLGRVACGFFSEMVLIKYKFPLMAGFLSE 420
           TAIDNLGQIG+          TFVTLVS+WNYLGRVA GF SE+V               
Sbjct: 361 TAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIV--------------- 420

Query: 421 HLLIKYKFPRPLMLTIVLLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSE 480
             L KYKFPRPLML++ LLLSC+ HL+IAF    GLY+AS++ GFC+GAQWPLLFAI+SE
Sbjct: 421 --LTKYKFPRPLMLSLTLLLSCVGHLIIAFAVPNGLYVASIVIGFCFGAQWPLLFAIISE 480

Query: 481 IFGLKYYATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMTGRIRKTGEELICNGT 540
           +FGLKYY+TLYNFGSVASP+GLYVLNV VAG LYDKEA KQLA  G +RKTGEEL C G 
Sbjct: 481 LFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNLYDKEAAKQLAAKGLVRKTGEELKCYGG 540

Query: 541 VCFKLSFVIITAVSLFGALVSLVLVLRTKKFYKSDIYKKFREAEEAAAVEEEEDDGRVVR 564
            CFK SF++IT V+L G  VSL+LV+RT+ FYKSDIYKKFR+  EA  V      G    
Sbjct: 541 ECFKRSFLVITGVTLLGMFVSLILVIRTRGFYKSDIYKKFRDEAEAEVV------GNGAV 587

BLAST of PI0000875 vs. ExPASy TrEMBL
Match: A0A6J1K186 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489721 PE=4 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 1.8e-198
Identity = 381/578 (65.92%), Postives = 444/578 (76.82%), Query Frame = 0

Query: 1   MLAGGRILGCSHIRTIVVHIITGRWLVLFFSLLLMAVAGATYMFSLYSNDIKLIW----- 60
           M+AGG I GCS I +I VH+ITGRW V+F SLL+MA AGATYMFSLYS+DIK +      
Sbjct: 1   MVAGGGISGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQT 60

Query: 61  ---------DLGANVGILSGLINEVTPAWVVLSIGAVMNFFGYFMIWLTITRRTSAPKVW 120
                    DLGANVG+LSGLINEVTP WVVLSIGAVMNFFGYFMIWL +T + SAPKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTGKISAPKVW 120

Query: 121 QMCLYICIGANSQSFANTGSMVTCVKNFPESRGAVLGILKGYIGLSGAIITQLFHAFYGA 180
           QMCLYICIGANSQSFANTGS+VTCVKNFPESRG VLGILKGY+GLSGAIITQLFHAFYG 
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGG 180

Query: 181 DTKSLILFIGWLPTAISFASLPIIRIMKVIRQPNELKVFYNFLYISLSLAGFLMLLIILE 240
           DTKSLIL IGWLP AISFA L  IRIMKVIRQPNELKVFYNFLYISL+LAGFLML+II+E
Sbjct: 181 DTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIE 240

Query: 241 SKTEFTQTNTVAAP---------PSSSSFSSSHS-----SSSSSKNTISGNSKPLSL-NL 300
           SKTEF+Q+    +          P +      ++     +++++   I   + P+ +   
Sbjct: 241 SKTEFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAAIRSPNHPVQIVTE 300

Query: 301 QLPLQE-QKEPSCWTTIFSPPERGEDFTILQAFFSVDMLILFTSAACGIGGTLTAIDNLG 360
           ++P  E QK+PSCWTTIF+PPERGEDFTILQA FSVDM ILF +A CG+GGTLTAIDNLG
Sbjct: 301 KVPKTEHQKQPSCWTTIFNPPERGEDFTILQALFSVDMFILFLAAICGVGGTLTAIDNLG 360

Query: 361 QIGI----------TFVTLVSVWNYLGRVACGFFSEMVLIKYKFPLMAGFLSEHLLIKYK 420
           QIG+          TFVTLVS+WNYLGRV  GF SE+V                 L KYK
Sbjct: 361 QIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIV-----------------LTKYK 420

Query: 421 FPRPLMLTIVLLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEIFGLKYY 480
           FPRPLML++ LL+SC+ HL+IAF+   GLY+AS++ GFC+GAQWPL+FAI+SE+FGLKYY
Sbjct: 421 FPRPLMLSMTLLVSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYY 480

Query: 481 ATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMTGRIRKTGEELICNGTVCFKLSF 539
           +TLYNFGSVASP+GLYVLNV VAG  YD+EA+KQL   G +RKTGEEL C G  CFKLSF
Sbjct: 481 STLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSF 540

BLAST of PI0000875 vs. NCBI nr
Match: XP_004142500.2 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus] >KAE8648848.1 hypothetical protein Csa_009434 [Cucumis sativus])

HSP 1 Score: 716.8 bits (1849), Expect = 1.4e-202
Identity = 396/603 (65.67%), Postives = 448/603 (74.30%), Query Frame = 0

Query: 1   MLAGGRILGCSHIRTIVVHIITGRWLVLFFSLLLMAVAGATYMFSLYSNDIK-------- 60
           MLAG RILGC  IR+IVVH+IT RW VLF SLL+M VAG+TY+FSLYSNDIK        
Sbjct: 1   MLAGERILGCFDIRSIVVHVITRRWFVLFASLLIMGVAGSTYIFSLYSNDIKSTLGYDQT 60

Query: 61  ------LIWDLGANVGILSGLINEVTPAWVVLSIGAVMNFFGYFMIWLTITRRTSAPKVW 120
                    DLGAN+G+LSGLINEVTP WVVLSIGA+MNFFGYFMIWL IT R S PKVW
Sbjct: 61  TLNLLSFSKDLGANIGVLSGLINEVTPPWVVLSIGALMNFFGYFMIWLAITNRISTPKVW 120

Query: 121 QMCLYICIGANSQSFANTGSMVTCVKNFPESRGAVLGILKGYIGLSGAIITQLFHAFYGA 180
           QMCLYICIGANSQSFANTGSMVTCV NFPESRG VLGILKGY GLSGAIITQLFHAFYGA
Sbjct: 121 QMCLYICIGANSQSFANTGSMVTCVMNFPESRGVVLGILKGYAGLSGAIITQLFHAFYGA 180

Query: 181 DTKSLILFIGWLPTAISFASLPIIRIMKVIRQPNELKVFYNFLYISLSLAGFLMLLIILE 240
           DTKSL+LFIGWLPTA+SFASL IIRI+KVIRQPNELKVFYNFLYISL+LAGFLML+II+E
Sbjct: 181 DTKSLVLFIGWLPTAVSFASLRIIRIIKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKTEFTQTN----------------TVAAPPSSSSFSSSHSSSSSSKNTISGNSKPLSLN 300
           SKTEFTQ                   +        +    +   S   ++   ++ L   
Sbjct: 241 SKTEFTQNQYGGSAAVVLLLLLLPLLIVVMEEYKLWKLKTALIQSPNPSVQIVTEQLP-K 300

Query: 301 LQLPLQEQKEPSCWTTIFSPPERGEDFTILQAFFSVDMLILFTSAACGIGGTLTAIDNLG 360
            + P QE KEPSCWTTIFSPP+RGEDFTILQ  FSVDMLILFTSAACG+GGTLTAIDNLG
Sbjct: 301 TEHPKQEHKEPSCWTTIFSPPKRGEDFTILQGLFSVDMLILFTSAACGMGGTLTAIDNLG 360

Query: 361 QIGI----------TFVTLVSVWNYLGRVACGFFSEMVLIKYKFPLMAGFLSEHLLIKYK 420
           QIG+          TFVTLVS+WNYLGRVACGF SE+V                 L KYK
Sbjct: 361 QIGVSLGYPKRSISTFVTLVSIWNYLGRVACGFLSEIV-----------------LRKYK 420

Query: 421 FPRPLMLTIVLLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEIFGLKYY 480
            PR L+L+++LLLSC+ HL+IAF+   GLY+AS++ GFC+GAQWPL+FAI+SE+FGLKYY
Sbjct: 421 CPRTLILSLILLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYY 480

Query: 481 ATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMTGRIRKTGEELICNGTVCFKLSF 540
           +TLYNFGSVASP+GLYVLNV VAG  YD+EA+KQL   G IRK GEEL C G  CFKLSF
Sbjct: 481 STLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLEAKGIIRKAGEELKCIGGACFKLSF 540

Query: 541 VIITAVSLFGALVSLVLVLRTKKFYKSDIYKKFREAEEAAAVEEEEDDGRVVRNGGGLME 564
           ++IT V+L G LVSL+LV+RT+ FY+SDIYKKFRE  E   V       RVV   G   E
Sbjct: 541 IVITGVTLLGMLVSLILVIRTRSFYRSDIYKKFREEVETTEVA----GNRVVEAAGETEE 581

BLAST of PI0000875 vs. NCBI nr
Match: XP_038893353.1 (LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 706.8 bits (1823), Expect = 1.5e-199
Identity = 388/607 (63.92%), Postives = 451/607 (74.30%), Query Frame = 0

Query: 1   MLAGGRILGCSHIRTIVVHIITGRWLVLFFSLLLMAVAGATYMFSLYSNDIKLIW----- 60
           M+AGG ILGCS +R+I VH+ITGRW V+F SLL+MA AGATYMF LYS+DIK +      
Sbjct: 1   MVAGGGILGCSDMRSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSDDIKSVLGYDQT 60

Query: 61  ---------DLGANVGILSGLINEVTPAWVVLSIGAVMNFFGYFMIWLTITRRTSAPKVW 120
                    DLGANVG+LSGLINEVTP WVVLSIGA++NFFGYFMIWL +TRR SAPKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAILNFFGYFMIWLAVTRRISAPKVW 120

Query: 121 QMCLYICIGANSQSFANTGSMVTCVKNFPESRGAVLGILKGYIGLSGAIITQLFHAFYGA 180
           QMC+YICIGANSQSFANTGS+VTCVKNFPESRG VLGILKGY+GLSGAIITQLFHAFYG 
Sbjct: 121 QMCVYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGN 180

Query: 181 DTKSLILFIGWLPTAISFASLPIIRIMKVIRQPNELKVFYNFLYISLSLAGFLMLLIILE 240
           DTKSLIL IGWLP AISFA L  IRI+KVIR PNELKVFYNFLYISL LAGFLML+II+E
Sbjct: 181 DTKSLILLIGWLPAAISFAFLRTIRIIKVIRHPNELKVFYNFLYISLGLAGFLMLMIIIE 240

Query: 241 SKTEFTQTN------------------TVAAPPSSSSFSSSHSSSSSSKNTISGNSKPLS 300
           SKTEFTQ                     +    +     ++   SS+    I     P +
Sbjct: 241 SKTEFTQNEYGGSAAVVLVLLFLPLAVVIIEEYNLWKLKTAFIKSSNPCVQIVTEQLPKT 300

Query: 301 LN-LQLPLQEQKEPSCWTTIFSPPERGEDFTILQAFFSVDMLILFTSAACGIGGTLTAID 360
            +  Q P QEQKEPSCWTT+FSPPERGEDFTILQA FS+DMLILF SA CG+GGTLTAID
Sbjct: 301 EHPKQTPKQEQKEPSCWTTVFSPPERGEDFTILQALFSIDMLILFLSATCGVGGTLTAID 360

Query: 361 NLGQIGI----------TFVTLVSVWNYLGRVACGFFSEMVLIKYKFPLMAGFLSEHLLI 420
           NLGQIG+          TFV+LVS+WNYLGR+A GF SE+V                 + 
Sbjct: 361 NLGQIGMSLGYPKRSISTFVSLVSIWNYLGRIASGFVSEIV-----------------VT 420

Query: 421 KYKFPRPLMLTIVLLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEIFGL 480
           KYKFPRPL+L++ LLLSC+ HL+IAF+   GLY+ASV+ GFC+GAQWP++FAI+SE+FGL
Sbjct: 421 KYKFPRPLILSLTLLLSCVGHLMIAFDVPNGLYVASVVIGFCFGAQWPVIFAIISELFGL 480

Query: 481 KYYATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMTGRIRKTGEELICNGTVCFK 540
           KYY+TLYNFGSVASP+GLY+LNV VAG  YD+EA+KQL   G  RK G++L C G  CFK
Sbjct: 481 KYYSTLYNFGSVASPIGLYILNVKVAGNFYDREAEKQLRAKGITRKAGKKLKCFGGECFK 540

Query: 541 LSFVIITAVSLFGALVSLVLVLRTKKFYKSDIYKKFREAEEAAAVEEEEDDGRVVRNGGG 565
           LSF++ITAV+L G  +SL+LV+RTK FYKSDIYKKFR+  E     E   +G V   G G
Sbjct: 541 LSFIVITAVTLLGTFISLILVIRTKSFYKSDIYKKFRDEAET----EVAGNGAVAPAGAG 584

BLAST of PI0000875 vs. NCBI nr
Match: XP_011655862.2 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KAE8648847.1 hypothetical protein Csa_008229 [Cucumis sativus])

HSP 1 Score: 706.8 bits (1823), Expect = 1.5e-199
Identity = 389/604 (64.40%), Postives = 451/604 (74.67%), Query Frame = 0

Query: 1   MLAGGRILGCSHIRTIVVHIITGRWLVLFFSLLLMAVAGATYMFSLYSNDIKLIW----- 60
           MLA GRI+GC  + +IVVHIITGRW +LF SLL+MAV+GATYMFSLYSND+K I      
Sbjct: 1   MLASGRIVGCFDMGSIVVHIITGRWFILFASLLIMAVSGATYMFSLYSNDLKSILGYDQT 60

Query: 61  ---------DLGANVGILSGLINEVTPAWVVLSIGAVMNFFGYFMIWLTITRRTSAPKVW 120
                    DLGANVGILSGL+N+VTP WVVLSIGA+MNFFGYFMIWL IT R S PKVW
Sbjct: 61  TLNLLSFSKDLGANVGILSGLVNQVTPPWVVLSIGAIMNFFGYFMIWLAITNRISTPKVW 120

Query: 121 QMCLYICIGANSQSFANTGSMVTCVKNFPESRGAVLGILKGYIGLSGAIITQLFHAFYGA 180
           QMCLYICIGANSQSF+NTGS+VTCVKNFP+SRGAVLGILKGY+GLSGAIITQLFHAFYGA
Sbjct: 121 QMCLYICIGANSQSFSNTGSIVTCVKNFPQSRGAVLGILKGYVGLSGAIITQLFHAFYGA 180

Query: 181 DTKSLILFIGWLPTAISFASLPIIRIMKVIRQPNELKVFYNFLYISLSLAGFLMLLIILE 240
           D KSLILFI WLPTAIS ASLPIIRIMK IRQPNELKVFYNFLYISL+LAGFLML+II+E
Sbjct: 181 DEKSLILFIAWLPTAISCASLPIIRIMKFIRQPNELKVFYNFLYISLALAGFLMLVIIVE 240

Query: 241 SKTEFTQTN------TVAAPPSSSSFSSSHSSSSSSKNTISGNSKPLSL------NLQLP 300
           SKT+FTQ         V   P        ++        I  ++  + +        + P
Sbjct: 241 SKTKFTQNQYGGIVAVVLLLPLVIVVMEEYNLWKLKTTLIKSSNPSVQIVTEQLPKTEHP 300

Query: 301 LQEQKEPSCWTTIFSPPERGEDFTILQAFFSVDMLILFTSAACGIGGTLTAIDNLGQIGI 360
            QEQKEPSCW TIFSPPERGEDFTILQ  FSVDMLIL TS ACG+GGTLTAIDNLGQIG+
Sbjct: 301 KQEQKEPSCWRTIFSPPERGEDFTILQGLFSVDMLILLTSVACGLGGTLTAIDNLGQIGM 360

Query: 361 T----------FVTLVSVWNYLGRVACGFFSEMVLIKYKFPLMAGFLSEHLLIKYKFPRP 420
           +          FVTLVS+W+YLGRV CGF SE+V                 LIKYKFPR 
Sbjct: 361 SLGYPKKSISIFVTLVSIWSYLGRVGCGFISEIV-----------------LIKYKFPRT 420

Query: 421 LMLTIVLLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEIFGLKYYATLY 480
           L+L+++LLLSC+ HL+IAF+   GLY+AS++ GFC+GAQWPL+FAI+SE+FGLKYYATL+
Sbjct: 421 LILSLILLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLVFAIISELFGLKYYATLH 480

Query: 481 NFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMTGRIRKTGEELICNGTVCFKLSFVIIT 540
            FG+VASPVGLYVLNV VAG  YD+EA+KQL   G +RK G+EL C G  CFKLSF +IT
Sbjct: 481 VFGAVASPVGLYVLNVKVAGNFYDREARKQLNTKGILRKAGKELNCYGGECFKLSFTVIT 540

Query: 541 AVSLFGALVSLVLVLRTKKFYKSDIYKKFREAEEAAAVEEEEDDGRVVRNGGGLMEETKQ 569
           AV+L G  +SL+LV+RT+ FYKSDIYKKFR+  E       E  G       G +EET++
Sbjct: 541 AVTLMGMFISLILVIRTRSFYKSDIYKKFRDEAET------EVAGNEAVATAGAVEETEK 581

BLAST of PI0000875 vs. NCBI nr
Match: XP_022956604.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 704.9 bits (1818), Expect = 5.6e-199
Identity = 392/612 (64.05%), Postives = 455/612 (74.35%), Query Frame = 0

Query: 1   MLAGGRILGCSHIRTIVVHIITGRWLVLFFSLLLMAVAGATYMFSLYSNDIKLIW----- 60
           M+AGG I GCS I +I VH+ITGRW V+F SLL+MA AGATYMFSLYS+DIK +      
Sbjct: 1   MVAGGGISGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQT 60

Query: 61  ---------DLGANVGILSGLINEVTPAWVVLSIGAVMNFFGYFMIWLTITRRTSAPKVW 120
                    DLGANVG+LSGLINEVTP WVVLSIGAVMNFFGYFMIWL +TR+ +APKVW
Sbjct: 61  TLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTRKIAAPKVW 120

Query: 121 QMCLYICIGANSQSFANTGSMVTCVKNFPESRGAVLGILKGYIGLSGAIITQLFHAFYGA 180
           QMCLYICIGANSQSFANTGS+VTCVKNFPESRG VLGILKGY+GLSGAIITQLFHAFYG 
Sbjct: 121 QMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGG 180

Query: 181 DTKSLILFIGWLPTAISFASLPIIRIMKVIRQPNELKVFYNFLYISLSLAGFLMLLIILE 240
           DTKSLIL IGWLP AISFA L  IRIMKVIRQPNELKVFYNFLYISL+LAGFLML+II+E
Sbjct: 181 DTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVE 240

Query: 241 SKTEFTQTN-------------TVAAPPSSSSFSSSHSSSSSSKNTISGNSKPLSL-NLQ 300
           SKTEF+Q+                 A      ++  +  ++++   I   + P+ +   +
Sbjct: 241 SKTEFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAIRSPNHPVQIVTEK 300

Query: 301 LPLQE----------QKEPSCWTTIFSPPERGEDFTILQAFFSVDMLILFTSAACGIGGT 360
           LP  E          QKEPSCWTTIF+PPERGEDFTILQA FSVDMLILF +A CG+GGT
Sbjct: 301 LPKTEHQKHIPKTEHQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLAAICGVGGT 360

Query: 361 LTAIDNLGQIGI----------TFVTLVSVWNYLGRVACGFFSEMVLIKYKFPLMAGFLS 420
           LTAIDNLGQIG+          TFVTLVS+WNYLGRV  GF SE+V              
Sbjct: 361 LTAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIV-------------- 420

Query: 421 EHLLIKYKFPRPLMLTIVLLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVS 480
              L KYKFPRPLML++ LL+SC+ HL+IAF+   GLY+AS++ GFC+GAQWPL+FAI+S
Sbjct: 421 ---LTKYKFPRPLMLSMTLLVSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIIS 480

Query: 481 EIFGLKYYATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMTGRIRKTGEELICNG 540
           E+FGLKYY+TLYNFGSVASP+GLYVLNV VAG  YD+EA+KQL   G +RKTGEEL C G
Sbjct: 481 ELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFG 540

Query: 541 TVCFKLSFVIITAVSLFGALVSLVLVLRTKKFYKSDIYKKFREAEEAAAVEEEEDDGRVV 565
             CFKLSF++IT V+L G  VSL+LV+RT+ FYKSDIYKKFR+  E     E   +G VV
Sbjct: 541 GECFKLSFLVITGVTLLGMFVSLILVIRTRSFYKSDIYKKFRDEAET----EMAGNGAVV 589

BLAST of PI0000875 vs. NCBI nr
Match: KAG6573851.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 704.5 bits (1817), Expect = 7.3e-199
Identity = 390/601 (64.89%), Postives = 444/601 (73.88%), Query Frame = 0

Query: 5   GRILGCSHIRTIVVHIITGRWLVLFFSLLLMAVAGATYMFSLYSNDIKLIW--------- 64
           G I G S+IRTI VHIITGRW V+F SLL+MA AGATYMFSLYSNDIK +          
Sbjct: 10  GAISGSSNIRTIAVHIITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVLGYDQTTLNL 69

Query: 65  -----DLGANVGILSGLINEVTPAWVVLSIGAVMNFFGYFMIWLTITRRTSAPKVWQMCL 124
                DLGANVGILSGLINEVTP WVVLSIGAV+NFFGYFMIWL +T+R S PKVWQMCL
Sbjct: 70  LSFFKDLGANVGILSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRISTPKVWQMCL 129

Query: 125 YICIGANSQSFANTGSMVTCVKNFPESRGAVLGILKGYIGLSGAIITQLFHAFYGADTKS 184
           YIC+GANSQSFANTGS+VTCVKNFP+SRG VLGILKGY+GLSGAIITQLFHAFYG+DTKS
Sbjct: 130 YICVGANSQSFANTGSLVTCVKNFPQSRGVVLGILKGYVGLSGAIITQLFHAFYGSDTKS 189

Query: 185 LILFIGWLPTAISFASLPIIRIMKVIRQPNELKVFYNFLYISLSLAGFLMLLIILESKTE 244
           LIL IGWLP AISFA L  IRIMKVIRQPNELKVFYNFLYISL+LAGFLML+II+ESK  
Sbjct: 190 LILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESKLI 249

Query: 245 FTQTN------------------TVAAPPSSSSFSSSHSSSSSSKNTISGNSKPLSLNLQ 304
           F+Q                     +    + S   S+  +  +    ++    P     Q
Sbjct: 250 FSQAEFGGSAAVVLLLLFLPLAVVIVEEYNLSKLKSAAINPPNPLQILTEKPNPPKTEPQ 309

Query: 305 LPLQEQKEPSCWTTIFSPPERGEDFTILQAFFSVDMLILFTSAACGIGGTLTAIDNLGQI 364
              ++QK+PSCWTTIFSPPERGEDFTILQA FS+DM ILF SA CG+GGTLTAIDNLGQI
Sbjct: 310 KQTRQQKQPSCWTTIFSPPERGEDFTILQALFSLDMAILFFSAICGVGGTLTAIDNLGQI 369

Query: 365 GI----------TFVTLVSVWNYLGRVACGFFSEMVLIKYKFPLMAGFLSEHLLIKYKFP 424
           G+          TFVTLVS+WNYLGRVA GF SE+V                 L KYKFP
Sbjct: 370 GMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIV-----------------LTKYKFP 429

Query: 425 RPLMLTIVLLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEIFGLKYYAT 484
           RPLML++ LLLSC+ HL+IAF    GLY+AS++ GFC+GAQWPLLFAI+SE+FGLKYY+T
Sbjct: 430 RPLMLSLTLLLSCVGHLIIAFAVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYST 489

Query: 485 LYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMTGRIRKTGEELICNGTVCFKLSFVI 544
           LYNFGSVASP+GLYVLNV VAG LYDKEA KQLA  G +RKTGEEL C G  CFK SF++
Sbjct: 490 LYNFGSVASPIGLYVLNVKVAGNLYDKEAAKQLAAKGLVRKTGEELKCYGGECFKRSFLV 549

Query: 545 ITAVSLFGALVSLVLVLRTKKFYKSDIYKKFREAEEAAAVEEEEDDGRVVRNGGGLMEET 564
           IT V+L G  VSL+LV+RT+ FYKSDIYKKFR+  EA    E   +G V     G+ EET
Sbjct: 550 ITGVTLLGMFVSLILVIRTRGFYKSDIYKKFRDEAEA----EVAGNGAVA--AAGVPEET 587

BLAST of PI0000875 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 633.3 bits (1632), Expect = 1.9e-181
Identity = 354/609 (58.13%), Postives = 421/609 (69.13%), Query Frame = 0

Query: 13  IRTIVVHIITGRWLVLFFSLLLMAVAGATYMFSLYSNDIK--------------LIWDLG 72
           ++++ + I+TGRW + F SLL+M+ AGATYMF +YS DIK                 DLG
Sbjct: 10  MKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLG 69

Query: 73  ANVGILSGLINEVTPAWVVLSIGAVMNFFGYFMIWLTITRRTSAPKVWQMCLYICIGANS 132
           ANVG+L+GL+NEVTP W +L IGA++NFFGYFMIWL +T R S P+VW MCLYIC+GANS
Sbjct: 70  ANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANS 129

Query: 133 QSFANTGSMVTCVKNFPESRGAVLGILKGYIGLSGAIITQLFHAFYGADTKSLILFIGWL 192
           QSFANTGS+VTCVKNFPESRG VLGILKGY+GLSGAIITQL+ AFYG DTK LIL IGWL
Sbjct: 130 QSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWL 189

Query: 193 PTAISFASLPIIRIMKVIRQPNELKVFYNFLYISLSLAGFLMLLIILESKTEFTQTN--- 252
           P  +SFA L  IRIMKV RQ NELKVFYNFLYISL LA FLM++II+   + FTQ+    
Sbjct: 190 PAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGG 249

Query: 253 -----TVAAPPSSSSFSSSHSSSSSSKNTISGNSKPLSLNLQLP---------------- 312
                 V                   K     +  P+++  + P                
Sbjct: 250 SAAVVIVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESK 309

Query: 313 --LQEQKEPSCWTTIFSPPERGEDFTILQAFFSVDMLILFTSAACGIGGTLTAIDNLGQI 372
             +++ K PSCWTT+F+PPERG+D+TILQA FSVDMLILF +  CG+GGTLTAIDNLGQI
Sbjct: 310 EVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQI 369

Query: 373 G----------ITFVTLVSVWNYLGRVACGFFSEMVLIKYKFPLMAGFLSEHLLIKYKFP 432
           G           TFV+LVS+WNY GRV                 ++G +SE  LIKYKFP
Sbjct: 370 GNSLGYPKRSVSTFVSLVSIWNYYGRV-----------------VSGVVSEIFLIKYKFP 429

Query: 433 RPLMLTIVLLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEIFGLKYYAT 492
           RPLMLT+VLLLSC  HLLIAFN  GGLY+ASV+ GFC+GAQWPLLFAI+SEIFGLKYY+T
Sbjct: 430 RPLMLTMVLLLSCAGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYST 489

Query: 493 LYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMTGRIRKTGEELICNGTVCFKLSFVI 552
           LYNFGSVASP+G Y+LNV VAGYLYD EA KQ    G+ R  G++L C GT CFKLSF+I
Sbjct: 490 LYNFGSVASPIGSYLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFII 549

Query: 553 ITAVSLFGALVSLVLVLRTKKFYKSDIYKKFREAEEAAAVE-------------EEEDD- 557
           I AV+LFG LVS+VLV+RTKKFYKSDIYKKFRE   AA +E             E++DD 
Sbjct: 550 IAAVTLFGVLVSMVLVIRTKKFYKSDIYKKFREKALAAEMEMAAPAAARSTVAKEDKDDV 601

BLAST of PI0000875 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 548.9 bits (1413), Expect = 4.8e-156
Identity = 308/568 (54.23%), Postives = 385/568 (67.78%), Query Frame = 0

Query: 17  VVHIITGRWLVLFFSLLLMAVAGATYMFSLYSNDIK--------------LIWDLGANVG 76
           ++H   GRW ++F S L+MA AGATY+F  YS DIK                 DLGANVG
Sbjct: 10  LIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVG 69

Query: 77  ILSGLINEVTPAWVVLSIGAVMNFFGYFMIWLTITRRTSAPKVWQMCLYICIGANSQSFA 136
           +LSGLI EVTP W VL+IG+ MNF GYFMIWLT+T + + PKVWQMCLYICIGANSQ+FA
Sbjct: 70  VLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFA 129

Query: 137 NTGSMVTCVKNFPESRGAVLGILKGYIGLSGAIITQLFHAFYGADTKSLILFIGWLPTAI 196
           NTG++VTCVKNFPESRG +LG+LKGY+GLSGAI TQL+ A YG D+KSLIL I WLP A+
Sbjct: 130 NTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAAV 189

Query: 197 SFASLPIIRIMKVIRQPNELKVFYNFLYISLSLAGFLMLLIILESKTEFTQTNTVAAP-- 256
           S   + +IR  KV+RQ NEL VFY FLYIS+ LA FLM + I E +  F++    A+   
Sbjct: 190 SLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATI 249

Query: 257 -------PSSSSFSSSHSSSSSSKNTISGNS--------KPLSLN----LQLPLQEQKEP 316
                  P + S        +  K  I   S        K L L+     ++  +E++  
Sbjct: 250 CCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEKETK 309

Query: 317 SCWTTIFSPPERGEDFTILQAFFSVDMLILFTSAACGIGGTLTAIDNLGQIG-------- 376
           SC++T+FSPP RGED+TILQA  S DM+ILF +  CG+G +LTA+DNLGQIG        
Sbjct: 310 SCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNH 369

Query: 377 --ITFVTLVSVWNYLGRVACGFFSEMVLIKYKFPLMAGFLSEHLLIKYKFPRPLMLTIVL 436
              +FV+LVS+WNY GRV                  +GF+SE+LL KYK PRPLM+T+VL
Sbjct: 370 TVSSFVSLVSIWNYFGRV-----------------FSGFVSEYLLAKYKLPRPLMMTLVL 429

Query: 437 LLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEIFGLKYYATLYNFGSVAS 496
           LLSC  HLLIAF   G +YIAS+L GF +GAQ PLLFAI+SE+FGLKYY+TL+N G +AS
Sbjct: 430 LLSCAGHLLIAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLAS 489

Query: 497 PVGLYVLNVNVAGYLYDKEAKKQLAMTGRIRKTGEELICNGTVCFKLSFVIITAVSLFGA 540
           P+G Y+LNV V G LYDKEA KQL   G  RK  ++L C G+ C+KL F+I+ AV+ FGA
Sbjct: 490 PLGSYILNVRVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGA 549

BLAST of PI0000875 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 258.8 bits (660), Expect = 9.9e-69
Identity = 185/585 (31.62%), Postives = 289/585 (49.40%), Query Frame = 0

Query: 18  VHIITGRWLVLFFSLLLMAVAGATYMFSLYS--------------NDIKLIWDLGANVGI 77
           + I+  +W+ +  S+ +   +GA+Y F +YS              + + +  D+GAN G+
Sbjct: 1   MEILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGV 60

Query: 78  LSGLINEVTPA----------------WVVLSIGAVMNFFGYFMIWLTITRRTSAPKVWQ 137
            SGL+     +                WVVL++GA+  F GYF+IW ++T     P V  
Sbjct: 61  FSGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPL 120

Query: 138 MCLYICIGANSQSFANTGSMVTCVKNFPESRGAVLGILKGYIGLSGAIITQLFHAFYGAD 197
           MCL++ + A SQ+F NT ++V+ V+NF +  G  +GI+KG++GLSGAI+ QL+      D
Sbjct: 121 MCLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD 180

Query: 198 TKSLILFIGWLPTAISFASLPIIRIMKVIRQPNELKVFYNFLYISLSLAGFLMLLIILES 257
             S IL +   PT +S   +P++RI +     ++ K       +SL +A +LM++IIL++
Sbjct: 181 PASFILLLAVTPTVLSLLVMPLVRIYET-SVADDKKHLNGLSAVSLIIAAYLMIIIILKN 240

Query: 258 KTEFTQTNTV---------------------------AAPPSSSSFSSSHSSSSSSKNTI 317
               +    +                             P   S   SS  +++S   + 
Sbjct: 241 TFGLSSWANIVTLVCLLVMLALPLLIARRAQRDGMEKTVPHDYSPLISSPKATTSGNQSS 300

Query: 318 SGNSK---PLSLNLQLPLQEQKEPSCWTTIFSPPERGEDFTILQAFFSVDMLILFTSAAC 377
            G+SK    LS NL L LQ  K+ S W                         +LF +  C
Sbjct: 301 EGDSKVEAGLSENLNL-LQAMKKLSFW-------------------------LLFLAMIC 360

Query: 378 GIGGTLTAIDNLGQIG----------ITFVTLVSVWNYLGRVACGFFSEMVLIKYKFPLM 437
           G+G  L+ I+N+ QIG           + V+L S+WN+LGR                   
Sbjct: 361 GMGSGLSTINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFG----------------- 420

Query: 438 AGFLSEHLLIKYKFPRPLMLTIVLLLSCIAHLLIAFNPSGGLYIASVLTGFCYGAQWPLL 497
           AG+ S+ LL K  +PRPL++   L    I HL+IA    G LY+ SV+ G CYG+QW L+
Sbjct: 421 AGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLM 480

Query: 498 FAIVSEIFGLKYYATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLAMTGRIRKTGEE 533
             I SE+FG+++  T++N  SVASP+G Y+ +V + GY+YDK A            +GE 
Sbjct: 481 PTITSELFGIRHMGTIFNTISVASPIGSYIFSVRLIGYIYDKTA------------SGEG 529

BLAST of PI0000875 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 2.9e-68
Identity = 183/598 (30.60%), Postives = 295/598 (49.33%), Query Frame = 0

Query: 11  SHIRTIVVHIITGRWLVLFFSLLLMAVAGATYMFSLYSNDIK--------------LIWD 70
           S  R   V  I  RWLV   ++ + + AG  Y+F   S  IK              +  D
Sbjct: 3   STTREKFVSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKD 62

Query: 71  LGANVGILSGLINEVTPAWVVLSIGAVMNFFGYFMIWLTITRRTSAPKVWQMCLYICIGA 130
           LG +VG ++G ++E+ P W  L +GAV N  GY  +WL +T R     +W MC+ I +G 
Sbjct: 63  LGDSVGFIAGTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGN 122

Query: 131 NSQSFANTGSMVTCVKNFPESRGAVLGILKGYIGLSGAIITQLFHAFYGADTKSLILFIG 190
           N +++ NTG++V+ V+NFP+SRG V+GILKG+ GL GAII+Q++   + ++  SLIL + 
Sbjct: 123 NGETYFNTGALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVA 182

Query: 191 WLPTAISFASLPIIRIM---KVIRQPNELKVFYNFLY-ISLSLAGFLMLLIILESK---- 250
             P  +    +  IR +   K IR  +     + F+Y + L LA +LM +++++      
Sbjct: 183 VTPAVVVVCLMFFIRPVGGHKQIRPTDGAS--FTFIYGVCLLLAAYLMSVMLIQDLVVVS 242

Query: 251 ----TEFT------QTNTVAAPPSSSSFSSSHSSSSSSKNTISGNSKPLSLNLQLP---- 310
               T FT          +  P  +S F+ ++    + +  +    +     LQ P    
Sbjct: 243 HNVITVFTIVLFVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLIL 302

Query: 311 ------------------------------LQEQKEPSCWTTIFSPPERGEDFTILQAFF 370
                                         +Q   E +        P RGEDFT+ QA  
Sbjct: 303 SEVEDEKPKDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALV 362

Query: 371 SVDMLILFTSAACGIGGTLTAIDNLGQIGIT--------FVTLVSVWNYLGRVACGFFSE 430
             D  ++F S   G G  LT IDNLGQ+  +         V+++S+WN+LGR+  G+FSE
Sbjct: 363 KADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVLVSMISIWNFLGRIGGGYFSE 422

Query: 431 MVLIKYKFPLMAGFLSEHLLIKYKFPRPLMLTIVLLLSCIAHLLIAFNPSGGLYIASVLT 490
           +V                 +  Y +PRP+ + +  L+  + H+  A+   G +YI ++L 
Sbjct: 423 LV-----------------VRDYAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLI 482

Query: 491 GFCYGAQWPLLFAIVSEIFGLKYYATLYNFGSVASPVGLYVLNVNVAGYLYDKEAKKQLA 535
           G  YGA W ++ A  SE+FGLK +  LYNF ++A+P G  V +  +A  +YD+EA++Q  
Sbjct: 483 GLGYGAHWAIVPATASELFGLKKFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQ-- 542

BLAST of PI0000875 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 255.8 bits (652), Expect = 8.4e-68
Identity = 181/550 (32.91%), Postives = 292/550 (53.09%), Query Frame = 0

Query: 20  IITGRWLVLFFSLLLMAVAGATYMF----------SLYSND-----IKLIWDLGANVGIL 79
           ++  RWLV   ++ + +VAG  Y+F          SL  N      + +  +LG  +G +
Sbjct: 12  LVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFV 71

Query: 80  SGLINEVTPAWVVLSIGAVMNFFGYFMIWLTITRRTSAPKVWQMCLYICIGANSQSFANT 139
           SG ++EV+P+WVVL +GA  N FGY ++WL +T +     +W + + I +G N +++ NT
Sbjct: 72  SGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNT 131

Query: 140 GSMVTCVKNFPESRGAVLGILKGYIGLSGAIITQLFHAFYGADTKSLILFIGWLPTAISF 199
            S+V+C+ NFPESRG V+GILKG+ GLSGAI+TQ++  F  +   S+IL +   P  +  
Sbjct: 132 ASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYLMFNPSHDSSVILMVALGPPVVVL 191

Query: 200 ASLPIIRIMKVIRQPNELKVFYNFLYI---SLSLAGFLMLLIILESKTEFTQTNTVAA-- 259
           A L I+R ++   + N       FL I    + LA +L+ L++L+S  + TQT    +  
Sbjct: 192 ALLFIVRPVERSCRTNLRSDDLRFLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGA 251

Query: 260 -----------PPSSSSFSSSHSSSS-----SSKNTISGNSKPLSLNLQLPLQEQKEPSC 319
                       P SS F S ++ +S      + N     ++ L      P ++++ P  
Sbjct: 252 ILVIFMVVPVLVPFSSVFISGNNVTSVKPEEGTSNVDQHEARTLIERSDRPPEKKRAPCI 311

Query: 320 WTTIFSPPERGEDFTILQAFFSVDMLILFTSAACGIGGTLTAIDNLGQIGIT-------- 379
                     GEDFT+LQA    D  ++F S   G+G  +T IDNLGQI  +        
Sbjct: 312 ----------GEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKI 371

Query: 380 FVTLVSVWNYLGRVACGFFSEMVLIKYKFPLMAGFLSEHLLIKYKFPRPLMLTIVLLLSC 439
           FV+L+S+ N+LGRVA G+FSE++                 + K   PR L +++V  +  
Sbjct: 372 FVSLISISNFLGRVAGGYFSELI-----------------IRKLSLPRTLAMSVVQAIMS 431

Query: 440 IAHLLIAFNPSGGLYIASVLTGFCYGAQWPLLFAIVSEIFGLKYYATLYNFGSVASPVGL 499
           +  +  A +  G +Y+ +++ G  YGA W +  A VS+IFGLK + +LYNF   A P+G 
Sbjct: 432 LGLIYYAIDWPGKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGS 491

Query: 500 YVLNVNVAGYLYDKEAKKQLAMTGRIRKTGEELICNGTVCFKLSFVIITAVSLFGALVSL 526
           +V +  +A  +YD  A+KQ   T       E L+C G+VC+ ++  +++ + L   ++SL
Sbjct: 492 FVFSGVIASNIYDYYARKQAGPTTET----ESLVCTGSVCYSVTCSLMSMLCLMAMVLSL 530