PI0000519 (gene) Melon (PI 482460) v1

BLAST of PI0000519 vs. ExPASy Swiss-Prot
Match: F8S296 (Autophagy-related protein 2 OS=Arabidopsis thaliana OX=3702 GN=ATG2 PE=2 SV=1)

HSP 1 Score: 1369.0 bits (3542), Expect = 0.0e+00
Identity = 845/1956 (43.20%), Postives = 1131/1956 (57.82%), Query Frame = 0

Query: 1    MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL 60
            +FPWNIAK+AE  FS++AVKR+ KFLLKKKLG+ +LG+ID+DQLD+QL DGTIQLSDLA+
Sbjct: 2    VFPWNIAKSAEEAFSRWAVKRVVKFLLKKKLGKLILGDIDLDQLDIQLRDGTIQLSDLAI 61

Query: 61   NVDFLNEKVGTAATVIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLK-NVHMDCC 120
            NVD+LN+K    A ++ KEGSIGSLL++MPW + GC+VE++ LELVL+P L+ N      
Sbjct: 62   NVDYLNDKFD--APLVIKEGSIGSLLVKMPWKTNGCQVEVDELELVLAPRLESNKSSSNE 121

Query: 121  GAFSGGHNKNHHESR----KSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKII 180
             + S    ++ H  R    K E++++ NAAKS   D+HEGVKTVAK+VK  L SFH+KI 
Sbjct: 122  ASTSASTREDLHNIRLEIGKHENEMLMNAAKSASIDVHEGVKTVAKIVKWFLTSFHVKIK 181

Query: 181  NLIVAFDSFYDENKNRTEFDTTLVLRIADVECGTCVTEDGKLGMDAVESFLGISQLNNFV 240
            NLI+AFD  + + ++      TLVLR+ ++ECG    E       + ++FLGI++L N V
Sbjct: 182  NLIIAFDPDFGKKQSEAGPRPTLVLRMTEIECGIS-EEQVSANEVSPDNFLGINRLANCV 241

Query: 241  KFQGAMVEFLHMDDCDKAKTFSCMSAATSQMVLDHVPPNVATPFLTGGVGGFSGNLKLCI 300
            KFQGA+VE L+MDD D                 D    N  T  +TG  GGFSG+L   I
Sbjct: 242  KFQGAVVELLNMDDDD-----------DGDKTCDKKTSNDVTLIMTGVGGGFSGSLNFSI 301

Query: 301  PLRDGSLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNGDRYINNKVNESDY 360
            P ++GSLDI +VD D+S DPV+++ QP TI+  L L    W +  +        V+ SD+
Sbjct: 302  PWKNGSLDIRKVDADISIDPVEVRFQPSTIRWFLQL----WKTFTSFGSDCFPSVSHSDF 361

Query: 361  FEQASHRHSSALASAEMTPDETSPHCGGM------LPGSHLISNWVPLSVKSREKEKVEE 420
               +    ++ +    +TP  T    GG        P    I +W P S   +E++   E
Sbjct: 362  LTDSPTIPTNVM----VTPPATLSLSGGQELEHDTTPNLQFIPDWFPSSFSKKEED--GE 421

Query: 421  FDFGASVDQFFECLDEIRSTQSALGSSGMWN---SVFSAITAASSLASGSLHVPSELQPV 480
             D GASVDQFFEC D +RS QSA GS GMWN   SVF+AI AASSLASGSL +PSE Q V
Sbjct: 422  VDIGASVDQFFECFDAMRSYQSASGSQGMWNWTSSVFTAINAASSLASGSLLLPSEQQHV 481

Query: 481  ETNLRATISGISIVISFHDD-------NKFHFTDAEKVQIKADSEVHFVAAKF-SDIHLL 540
            ET+ + + +G+S+V+ F D+        + H+  AE   I    +V     +   +++ +
Sbjct: 482  ETSCKVSFAGVSVVLFFQDEVNWKGVSTRIHYLGAELRDISVSFQVCLHDLRLEGEVNSM 541

Query: 541  MQTDF-------RNSNGDFQTILMKRLQVDVLGALPPFEFSAEDPDLVESNGSFNMDLPC 600
               D+         +N + QT L+K LQ  V  +LPPF  S    D    +   +     
Sbjct: 542  EIADYCQGGNVVDTANAESQTCLIKDLQAKVQTSLPPFASSDMHSDSERLSEIVSDGFLF 601

Query: 601  ENKDNVTKITLLETYGITSSQLNTT-SSSNDNSTMTKSFSLNLPPFVFWVNYTLVNMLLD 660
             NK    K  L+   G +  Q      SS  +   + SFSL+LPP  FW+N   V ML++
Sbjct: 602  RNKGFAVKTLLVIAAGGSGFQFTVNFQSSKASHRGSNSFSLSLPPTTFWLNLHSVEMLVN 661

Query: 661  LLKDVANCMPGDNNHMCFKENYTSDHEDAKSSSNQVTA-LTLWAILPGI----------D 720
            L  DV+  +P   +H   +   +S  E  + S +   A + LW     I           
Sbjct: 662  LFNDVSESIP-ITSHERNQVASSSKSESLRGSVSICNARVILWFPFESISERFCNSLGQQ 721

Query: 721  IIALDF-YSPPITKEETTHRGNLALQKSYLSQKNALHFRFGSLGVFLVTFEEDIKQSSTC 780
             I +D   SPP  KE    R     +  + S   ++ F  G   ++LVT   D+K S T 
Sbjct: 722  FIVVDLSSSPPSDKERAKERS--PGEMHFPSATRSICFSVGDASIYLVT--SDLKDSETN 781

Query: 781  NLQRK-KFSAHNILSASNRTNG--SPLTLFWQEGHVTGPWIAKKAKSLASLEESKSRCKF 840
            +  R+ +FSA+NIL  +N+T    S + +FWQ+     PW+ ++AK LA+ EES    K 
Sbjct: 782  SYHRQVEFSAYNILHTNNKTRHQLSTIGMFWQDRPTVSPWLVERAKMLATQEESIQTDKS 841

Query: 841  IGKDYEFASVANMKDMED------------STSVLHVSFSLVRINVGTVQYEAFHCLLDQ 900
             G+  EFA+VA  KD +D            S+  L+V    + I++ + QY     L+++
Sbjct: 842  GGRGLEFAAVATPKDQDDIYSRSRKEIILASSFCLYVHLLPLAIHLDSWQYSKLCNLIEE 901

Query: 901  LVKGLSSETCDVVDVT-KGVACQTSILVDCNSLEIVIRPDSNESTKCSLQRELPGSWYHL 960
                LS    +  + T + V CQTS++VDC+S++I++RP+     K  LQ ELPGSW   
Sbjct: 902  AKNWLSRMAANTAEQTEESVVCQTSLVVDCDSIDILVRPEPRMGIKKQLQTELPGSWIQF 961

Query: 961  RLEIQNFELISVTDLGGIKGGNFFWLAHGEGKLLGFISEDPDQEFLLISCSNSNMKRGDG 1020
             L +Q   L+SV +LG + G +FFWLAHGEG LLG ++  PDQE LL+SC+NS +KRG+G
Sbjct: 962  NLRVQKLNLMSVPNLGSVSGADFFWLAHGEGTLLGSVTGLPDQELLLLSCNNSAIKRGNG 1021

Query: 1021 EGSNALSSRLAGCDIVHSWDPESLQGFSSVTIRCATIFAIGGRLDWLDVIYSFFFLSSPP 1080
             GSNALSSR AG D +H  +P     + +V+ R  TI A+GGRLDW++V  SFF      
Sbjct: 1022 GGSNALSSRFAGLDFLHLQEPGICNDYLAVSARGCTISAVGGRLDWIEVATSFFSF---- 1081

Query: 1081 VEPEDDKIMTRENPKKSSGSYFFLNFVDVGLSYHPYLKNLLVQSGLSQTESSSSTFKQEL 1140
               ED+K     N   SSGS F LNFVDVGLSY P+ +N              +   ++ 
Sbjct: 1082 ---EDEKKTQEINSSSSSGSSFILNFVDVGLSYEPHHEN--------------TDHLRQA 1141

Query: 1141 DDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHVFDAYSVEDLRK 1200
             D +VACL+AASS +LS  S+ D + ++YRI +QD GLLL    D   +   YS E L +
Sbjct: 1142 SDPWVACLVAASSFSLSKKSLVDSIRNDYRIRIQDLGLLLSVDFDLSKLGGTYSSEHLHE 1201

Query: 1201 VGYVKVARETCIEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLAAQLQQLFAP 1260
             GYVKVA ++ IEAILRTN  NGL WELEC K+H+ +ETC DT SGL RLA QLQQL AP
Sbjct: 1202 SGYVKVANDSLIEAILRTNSENGLLWELECSKSHLVIETCSDTTSGLIRLATQLQQLLAP 1261

Query: 1261 DLEESIVHLQTRWNNVQQGQERKEIDAG---SSSPPCHNLSVKQSE-------VGLMDEI 1320
            DLEES VHLQTRW+++QQ   R ++D     SSS     +   + E       +GLMDEI
Sbjct: 1262 DLEESAVHLQTRWDSIQQANARNDLDISDRLSSSDSSGEMKYLRLESENETGVIGLMDEI 1321

Query: 1321 CEDAFLLNKNDSKNVIIASQNVVCSSN-------------------SEVRETSSPAHSFM 1380
             EDAF  + N +      S +V C +N                    ++    S   S  
Sbjct: 1322 NEDAFQFDVNPT----YQSDSVECQNNYMSPHGISHGQAYNWVPATEKLPSNQSICGSSS 1381

Query: 1381 GSDPDGQTSFIQYRQFPEIIEGYCLSNLRSLPDL-----TIGRELHPEICNGRNSGSIDT 1440
              + +    F++    PEI E YCLS  R   ++     + GREL PE          D 
Sbjct: 1382 RINSESSQVFLERESLPEIFENYCLSEFRPSSEVPQEGDSSGRELFPE---------TDL 1441

Query: 1441 GGRRSGWYGDLPIKILENHVSDVSKVEHS--VTNDLCSSESKRLDEVEEASGRVLLNNID 1500
                SGWY D  ++I+E+HVS+ ++ +H   + +  CSS  +       A+GR+LL NID
Sbjct: 1442 RRGNSGWYDDASLRIVEDHVSEATEEDHEEHILDGECSSFGQTSYSAVAANGRILLKNID 1501

Query: 1501 VKWRMYAGSDWQVSRENGDPPMGMVKRDQHTCLELALSSMQ------------------- 1560
            +KWR+Y+GSDW  SR+ G+       RD  +CLEL LS +Q                   
Sbjct: 1502 LKWRIYSGSDWHDSRKKGENFKHTKGRDTTSCLELELSGVQFLYETFPIGEICTSKLSLM 1561

Query: 1561 -----------------VLGYYNSKNHPRKSSSKAFKLDLEAIRPDPLSLLR-------- 1620
                             VLGYYNSK+HPR SSS AFKL+L+A+RPDP + L         
Sbjct: 1562 VQDFYLYDRSDNAPWTLVLGYYNSKDHPRDSSSYAFKLELKAVRPDPETPLEENRLRVAL 1621

Query: 1621 ---------------------NTGQPPDSDASKTISSTK--SQDGLTLAEEALLPYFQKF 1680
                                 N+ + P      +  ST   S  G  + EEALLPYFQKF
Sbjct: 1622 LPILLHLHQSQLDFLISFFGANSLEKPVVSMGDSGGSTMSVSVQGHNIIEEALLPYFQKF 1681

Query: 1681 DIQPIVVRVDYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETV 1740
            DI P+ VRVDYSP  VD+AAL GGKY ELVNLVPWKG+EL LKHV A G+YGWG+VCET+
Sbjct: 1682 DIWPVNVRVDYSPHHVDIAALTGGKYAELVNLVPWKGIELQLKHVHAAGIYGWGNVCETI 1741

Query: 1741 VGEWLEDISHNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQRGTIA 1796
            +GEWLED+S NQI ++L+G+P VRSL A+ + A KLVSSPVESY+KDRR++KG+QRGT+A
Sbjct: 1742 LGEWLEDVSQNQIHQLLKGIPTVRSLSALYAAALKLVSSPVESYRKDRRLVKGVQRGTVA 1801

BLAST of PI0000519 vs. ExPASy Swiss-Prot
Match: P0CM31 (Autophagy-related protein 2 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=ATG2 PE=3 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 3.0e-39
Identity = 101/296 (34.12%), Postives = 157/296 (53.04%), Query Frame = 0

Query: 1515 YFQKFDIQPIVVRVDYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGS 1574
            YFQ  +I PI +++DY P RVD  ALR GK +EL+N   ++G E+ L+H+   G+ G   
Sbjct: 1645 YFQHVEIFPIQLKLDYKPKRVDFRALREGKTIELMNFFHFEGAEMTLRHITLSGITGLER 1704

Query: 1575 VCETVVGEWLEDISHNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQ 1634
            +  T+   W  D+  NQ+  ++ G+  +RS+V VGSG + L+  P+E Y+KD RI KG+Q
Sbjct: 1705 LGTTLQDLWTPDVKANQLADVISGVSPIRSMVNVGSGVADLILLPIEQYRKDGRIAKGVQ 1764

Query: 1635 RGTIAFLRSISLEAVGLGVHLAAGAHDILLQAEYIL-----TSIPPSVKVRHKTRPNVRS 1694
            RGT +F++S +LE + LG  LA G   IL +AE +L       +   V+        V S
Sbjct: 1765 RGTNSFVKSTALEVMKLGARLATGTQVILERAEGVLGGKSGEDVVGQVQGLSTNAFGVDS 1824

Query: 1695 --------------------NQPKDAQEGLKKAYESLSDGLGKSASALVRTPLKKYQRGA 1754
                                +QP+  +EG++ AY+SLS  +  +A  ++  P++ Y+R  
Sbjct: 1825 GMLEGGSSSEDEQEAISRYADQPESMKEGVQAAYKSLSKNVNAAAQTILAVPMEVYERSG 1884

Query: 1755 STVSAFATAVQAIPAAAIAPASACASAIHYTFLGLRNRSFLFSLDPERKRESMEKY 1786
                     ++A+P A + P      A+  T LG+RN     SLDP  +RE  +KY
Sbjct: 1885 DD-GPLKAVIRAVPIAVLKPMIGTTEAVSKTLLGMRN-----SLDPSARRELDDKY 1934

BLAST of PI0000519 vs. ExPASy Swiss-Prot
Match: P0CM30 (Autophagy-related protein 2 OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=ATG2 PE=3 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 3.0e-39
Identity = 101/296 (34.12%), Postives = 157/296 (53.04%), Query Frame = 0

Query: 1515 YFQKFDIQPIVVRVDYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGS 1574
            YFQ  +I PI +++DY P RVD  ALR GK +EL+N   ++G E+ L+H+   G+ G   
Sbjct: 1645 YFQHVEIFPIQLKLDYKPKRVDFRALREGKTIELMNFFHFEGAEMTLRHITLSGITGLER 1704

Query: 1575 VCETVVGEWLEDISHNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQ 1634
            +  T+   W  D+  NQ+  ++ G+  +RS+V VGSG + L+  P+E Y+KD RI KG+Q
Sbjct: 1705 LGTTLQDLWTPDVKANQLADVISGVSPIRSMVNVGSGVADLILLPIEQYRKDGRIAKGVQ 1764

Query: 1635 RGTIAFLRSISLEAVGLGVHLAAGAHDILLQAEYIL-----TSIPPSVKVRHKTRPNVRS 1694
            RGT +F++S +LE + LG  LA G   IL +AE +L       +   V+        V S
Sbjct: 1765 RGTNSFVKSTALEVMKLGARLATGTQVILERAEGVLGGKSGEDVVGQVQGLSTNAFGVDS 1824

Query: 1695 --------------------NQPKDAQEGLKKAYESLSDGLGKSASALVRTPLKKYQRGA 1754
                                +QP+  +EG++ AY+SLS  +  +A  ++  P++ Y+R  
Sbjct: 1825 GMLEGGSSSEDEQEAISRYADQPESMKEGVQAAYKSLSKNVNAAAQTILAVPMEVYERSG 1884

Query: 1755 STVSAFATAVQAIPAAAIAPASACASAIHYTFLGLRNRSFLFSLDPERKRESMEKY 1786
                     ++A+P A + P      A+  T LG+RN     SLDP  +RE  +KY
Sbjct: 1885 DD-GPLKAVIRAVPIAVLKPMIGTTEAVSKTLLGMRN-----SLDPSARRELDDKY 1934

BLAST of PI0000519 vs. ExPASy Swiss-Prot
Match: Q4PFE7 (Autophagy-related protein 2 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=ATG2 PE=3 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 4.5e-35
Identity = 100/287 (34.84%), Postives = 147/287 (51.22%), Query Frame = 0

Query: 1513 LPYFQKFDIQPIVVRVDYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGW 1572
            LP+ Q  ++ PI +++DY P RVD   LR GK +EL+N   ++G E+ L+HV   G+ GW
Sbjct: 1775 LPFVQFAEVLPIKIKLDYKPKRVDYNLLRQGKTIELMNFFHFEGSEMVLRHVTLRGINGW 1834

Query: 1573 GSVCETVVGEWLEDISHNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKG 1632
              + +T+   W  D+  NQ+   L GL  +RSLV VG+G + LV  P+E Y KD R+L+G
Sbjct: 1835 ARLFDTLNDIWTPDVKANQLADFLSGLGPIRSLVNVGAGLADLVLLPIEQYHKDGRVLRG 1894

Query: 1633 MQRGTIAFLRSISLEAVGLGVHLAAGAHDILLQAEYIL---------------------- 1692
            +QRG   F ++ +LEAV LG  LA G   IL QAE+IL                      
Sbjct: 1895 VQRGAAGFAKTTALEAVKLGARLATGTQVILEQAEHILGGERMEESITASAIGPESGQSF 1954

Query: 1693 TSIPPSVKVRHKTRPNVRS---NQPKDAQEGLKKAYESLSDGLGKSASALVRTPLKKYQ- 1752
             S+  SV V   +R    S    QP D ++ L +AY  L+D L  +A  ++  P+  +  
Sbjct: 1955 QSLSESVMVERMSRSGSVSRYAQQPLDMRDALAQAYSGLTDHLTSAAQTILAIPMDVFDA 2014

Query: 1753 ---RGASTV-----SAFATAVQAIPAAAIAPASACASAIHYTFLGLR 1766
                GA T      +     V+A+P A +  A   + AI  T  G++
Sbjct: 2015 SDLAGAPTTRSSEHTRSRPVVKAVPIAILRGAQGASHAIAKTMQGVQ 2061

BLAST of PI0000519 vs. ExPASy Swiss-Prot
Match: Q0CSI0 (Autophagy-related protein 2 OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=atg2 PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 1.9e-33
Identity = 91/281 (32.38%), Postives = 150/281 (53.38%), Query Frame = 0

Query: 1513 LPYFQKFDIQPIVVRVDYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGW 1572
            +P+ Q+ ++  I V++D+ P RVD A L+ G+  E +N     G ++ ++HV   G+ G+
Sbjct: 1805 IPFLQRVEVNSIPVKLDFKPKRVDYAGLQSGRTTEFMNFFVLDGADMVMRHVIIYGIAGF 1864

Query: 1573 GSVCETVVGEWLEDISHNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKG 1632
              + +T+   W+ DI  NQ+  +L GL  +RSLV VG G   LV  P+  Y+KD RI++ 
Sbjct: 1865 DKLGQTLNDIWMPDIKRNQLPSVLAGLAPIRSLVNVGGGVKDLVVVPMREYRKDGRIVRS 1924

Query: 1633 MQRGTIAFLRSISLEAVGLGVHLAAGAHDILLQAEYILTSIPPSVKVRHK--------TR 1692
            +Q+G  +F ++ S E V LG  LA G   +L  AE +LTS    V V  +         +
Sbjct: 1925 IQKGAWSFAKTTSNELVKLGAKLAIGTQTVLQGAEEMLTSPNAPVPVSEEDSTDEEEAKK 1984

Query: 1693 PNVRSNQPKDAQEGLKKAYESLSDGLGKSASALVRTPLKKYQRGASTVSAFATAVQAIPA 1752
             ++ ++QP    +GL+ A+  L   L  +  A+V  P +  + G++T +A A   +A P 
Sbjct: 1985 ISLYADQPIGVVQGLRGAFRGLERDLLLARDAIVAVPGEIVESGSATAAAKAVWKRA-PT 2044

Query: 1753 AAIAPASACASAIHYTFLGLRNRSFLFSLDPERKRESMEKY 1786
              + PA   + A+  T LG  N     +LDP  +R+  +KY
Sbjct: 2045 VVLRPAIGVSKAVGQTLLGAGN-----TLDPSNRRKMEDKY 2079

BLAST of PI0000519 vs. ExPASy TrEMBL
Match: A0A0A0LAA0 (Autophagy-related protein 2 OS=Cucumis sativus OX=3659 GN=Csa_3G207330 PE=3 SV=1)

HSP 1 Score: 3278.0 bits (8498), Expect = 0.0e+00
Identity = 1691/1947 (86.85%), Postives = 1731/1947 (88.91%), Query Frame = 0

Query: 1    MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL 60
            MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQL+DLAL
Sbjct: 1    MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLNDLAL 60

Query: 61   NVDFLNEKVGTAATVIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHMDCCG 120
            NVDFLNEKVGTAA+VIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHM+CCG
Sbjct: 61   NVDFLNEKVGTAASVIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHMNCCG 120

Query: 121  AFSGGHNKNHHESRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA 180
            AFSG H+ NHHESRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA
Sbjct: 121  AFSGSHSNNHHESRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA 180

Query: 181  FDSFYDENKNRTEFDTTLVLRIADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA 240
            FDSFYDENKNRTEFDTTLVLRIADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA
Sbjct: 181  FDSFYDENKNRTEFDTTLVLRIADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA 240

Query: 241  MVEFLHMDDCDKAKTFSCMSAATSQMVLDHVPPNVATPFLTGGVGGFSGNLKLCIPLRDG 300
            MVEFLHMDDCDKAKTF CMSAATSQMVLDHVP NVATPFLTGGVGGFSGNLKLCIPLRDG
Sbjct: 241  MVEFLHMDDCDKAKTFPCMSAATSQMVLDHVPSNVATPFLTGGVGGFSGNLKLCIPLRDG 300

Query: 301  SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNGDRYINNKVNESDYFEQAS 360
            SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKN D  INNKVNESDYFE+A 
Sbjct: 301  SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNSDGCINNKVNESDYFERAF 360

Query: 361  HRHSSALASAEMTPDETSPHCGGMLPGSHLISNWVPLSVKSREKEKVEEFDFGASVDQFF 420
            H HSSALASAE TPDETSPHCGGMLPGSHLISNWVPLSVKSREKEKVEEFDFGASVDQFF
Sbjct: 361  HSHSSALASAETTPDETSPHCGGMLPGSHLISNWVPLSVKSREKEKVEEFDFGASVDQFF 420

Query: 421  ECLDEIRSTQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV 480
            ECLDEIRSTQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV
Sbjct: 421  ECLDEIRSTQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV 480

Query: 481  ISFHDDNKFHFTDAEKVQIKADSEVHFVAAKFSDIHLLMQ-------------------- 540
            ISFHDDNK+HFTD EKVQIKADSEVHFVAAKFSD+HLLMQ                    
Sbjct: 481  ISFHDDNKYHFTDTEKVQIKADSEVHFVAAKFSDVHLLMQVSTQRTRFHGTIKHVEIADY 540

Query: 541  ---------TDFRNSNGDFQTILMKRLQVDVLGALPPFEFSAEDPDLVESNGSFNMDLPC 600
                     TDF NSNGDFQTILMKRLQVDVLGALPPF+FSAEDPDLVESN SFNMDLPC
Sbjct: 541  LNCNSYASKTDFCNSNGDFQTILMKRLQVDVLGALPPFDFSAEDPDLVESNSSFNMDLPC 600

Query: 601  ENKDNVTKITLLETYGITSSQLNTTSSSNDNSTMTKSFSLNLPPFVFWVNYTLVNMLLDL 660
            ENKDNV KITLLETYGITSSQLN TSSSNDNSTM+KSFSLNLPPFVFWVNYTLVNMLLDL
Sbjct: 601  ENKDNVAKITLLETYGITSSQLNMTSSSNDNSTMSKSFSLNLPPFVFWVNYTLVNMLLDL 660

Query: 661  LKDVANCMPGDNNHMCFKENYTSDHEDAKSSSNQVTALTLWAILPGIDI----------- 720
            LKDVANCMPGDNNHMCFKENYTSDHEDAKSS NQVTAL+  ++   + I           
Sbjct: 661  LKDVANCMPGDNNHMCFKENYTSDHEDAKSSPNQVTALSFSSMQGNVIISNARVIFCFPL 720

Query: 721  ---------------IALDFYSPPITKEETTHRGNLALQKSYLSQKNALHFRFGSLGVFL 780
                           IALDFY+ PITKEETTHRGNLA+QKSY  QKNALHFRFGS+GVFL
Sbjct: 721  ESDKDFMGYSSWDRFIALDFYASPITKEETTHRGNLAVQKSYQLQKNALHFRFGSVGVFL 780

Query: 781  VTFEEDIKQSSTCNLQRKKFSAHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLAS 840
            VTFEEDIKQSSTCNLQ KKFS HNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLA 
Sbjct: 781  VTFEEDIKQSSTCNLQGKKFSVHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLAC 840

Query: 841  LEESKSRCKFIGKDYEFASVANMKDMED------------STSVLHVSFSLVRINVGTVQ 900
            LEESKS CKFIGKDYEFASVANMKDME+            STSVLHVSF LVRINVGTVQ
Sbjct: 841  LEESKSSCKFIGKDYEFASVANMKDMEESNLQTRQEMILSSTSVLHVSFPLVRINVGTVQ 900

Query: 901  YEAFHCLLDQLVKGLSSETCDVVDVTKGVACQTSILVDCNSLEIVIRPDSNESTKCSLQR 960
            Y+AFHCLLDQL+KGLS ETCDVVDVTKGVACQTSI+VDCNSLEIVIRPD NESTKCSLQR
Sbjct: 901  YKAFHCLLDQLIKGLSRETCDVVDVTKGVACQTSIVVDCNSLEIVIRPDLNESTKCSLQR 960

Query: 961  ELPGSWYHLRLEIQNFELISVTDLGGIKGGNFFWLAHGEGKLLGFISEDPDQEFLLISCS 1020
            ELPGSWYHLRLEIQNFELISV+DLGGIKG NFFWLAHGEGKLLGFISEDPDQEFLLISCS
Sbjct: 961  ELPGSWYHLRLEIQNFELISVSDLGGIKGANFFWLAHGEGKLLGFISEDPDQEFLLISCS 1020

Query: 1021 NSNMKRGDGEGSNALSSRLAGCDIVHSWDPESLQGFSSVTIRCATIFAIGGRLDWLDVIY 1080
            NSNMKRGDGEGSNALSSRLAGCDIVH WDPESLQGFSSVTIRCATI AIGGRLDWLDVIY
Sbjct: 1021 NSNMKRGDGEGSNALSSRLAGCDIVHLWDPESLQGFSSVTIRCATILAIGGRLDWLDVIY 1080

Query: 1081 SFFFLSSPPVEPEDDKIMTRENPKKSSGSYFFLNFVDVGLSYHPYLKNLLVQSGLSQTES 1140
            SFFFLSSPPVEPE DKIMTRENPK SSGS FFLNFVDVGL+YHPYLKNLL++SGLSQ+ES
Sbjct: 1081 SFFFLSSPPVEPEGDKIMTRENPKNSSGSCFFLNFVDVGLNYHPYLKNLLIKSGLSQSES 1140

Query: 1141 SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHVFD 1200
            SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHVFD
Sbjct: 1141 SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHVFD 1200

Query: 1201 AYSVEDLRKVGYVKVARETCIEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA 1260
            AYSVEDLRKVGYVKVARET IEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA
Sbjct: 1201 AYSVEDLRKVGYVKVARETFIEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA 1260

Query: 1261 AQLQQLFAPDLEESIVHLQTRWNNVQQGQERKEIDAGSSSPPCHNLSVKQSEVGLMDEIC 1320
            AQLQQLFAPDLEESIVHLQTRWNN QQGQERKEIDA SSSPPCHNLSV QSEVGLMDEIC
Sbjct: 1261 AQLQQLFAPDLEESIVHLQTRWNNAQQGQERKEIDAESSSPPCHNLSVNQSEVGLMDEIC 1320

Query: 1321 EDAFLLNKNDSK-------------NVIIASQNVVCSSNSEVRETSSPAHSFMGSDPDGQ 1380
            EDAFLLNKN S+             N ++ ++  VCSSNSEV ETSSPAHSFMGSDPDGQ
Sbjct: 1321 EDAFLLNKNHSRECDYSKTKCSFSPNEVLHAE--VCSSNSEVCETSSPAHSFMGSDPDGQ 1380

Query: 1381 TSFIQYRQFPEIIEGYCLSNLRSLPDLTIGRELHPEICNGRNSGSIDTGGRRSGWYGDLP 1440
            TSFIQYRQFPEIIEGYCLSNL SLPDLTIGRELHP+ICNGRNSGSIDTGGRRSGWYGDLP
Sbjct: 1381 TSFIQYRQFPEIIEGYCLSNLCSLPDLTIGRELHPDICNGRNSGSIDTGGRRSGWYGDLP 1440

Query: 1441 IKILENHVSDVSKVEHSVTNDLCSSESKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVS 1500
            IKILENHVSDVSKVE+SVTNDLCS+ESK+LDEVEE SGRV+LNNIDVKWRMYAGSDWQVS
Sbjct: 1441 IKILENHVSDVSKVEYSVTNDLCSTESKKLDEVEEVSGRVILNNIDVKWRMYAGSDWQVS 1500

Query: 1501 RENGDPPMGMVKRDQHTCLELALSSMQ--------------------------------- 1560
             ENGDPPMGMVKRDQHTCLELAL+SMQ                                 
Sbjct: 1501 SENGDPPMGMVKRDQHTCLELALTSMQVQYDIFPVGGMCISRLSLSIQDFHLYDSSVDAP 1560

Query: 1561 ---VLGYYNSKNHPRKSSSKAFKLDLEAIRPDP-------------LSLL---------- 1620
               VLGYYNSKNHPRKSSSKAFKLDLEAIRPDP             L +L          
Sbjct: 1561 WKLVLGYYNSKNHPRKSSSKAFKLDLEAIRPDPSIPLEEYRLCIGILPMLLHLHQCQLDF 1620

Query: 1621 -------------RNTGQPPDSDASKTISSTKSQDGLTLAEEALLPYFQKFDIQPIVVRV 1680
                         R++GQP D D SKTIS+TKS DGLTLAEEALLPYFQKFDIQPIVVRV
Sbjct: 1621 LVNFFGERSSSRNRSSGQPLDLDGSKTISTTKSHDGLTLAEEALLPYFQKFDIQPIVVRV 1680

Query: 1681 DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS 1740
            DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS
Sbjct: 1681 DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS 1740

Query: 1741 HNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQRGTIAFLRSISLEA 1796
            HNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQRGTIAFLRSISLEA
Sbjct: 1741 HNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQRGTIAFLRSISLEA 1800

BLAST of PI0000519 vs. ExPASy TrEMBL
Match: A0A1S3C2I1 (Autophagy-related protein 2 OS=Cucumis melo OX=3656 GN=LOC103496161 PE=3 SV=1)

HSP 1 Score: 3255.7 bits (8440), Expect = 0.0e+00
Identity = 1681/1947 (86.34%), Postives = 1725/1947 (88.60%), Query Frame = 0

Query: 1    MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL 60
            MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL
Sbjct: 1    MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL 60

Query: 61   NVDFLNEKVGTAATVIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHMDCCG 120
            NVDFLNEKVGTAA+V FKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHM+ CG
Sbjct: 61   NVDFLNEKVGTAASVTFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHMNGCG 120

Query: 121  AFSGGHNKNHHESRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA 180
            AFSGGH+KNHH SRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA
Sbjct: 121  AFSGGHSKNHHGSRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA 180

Query: 181  FDSFYDENKNRTEFDTTLVLRIADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA 240
            FDSFYDENKNRTEFDTTLVLR+ADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA
Sbjct: 181  FDSFYDENKNRTEFDTTLVLRVADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA 240

Query: 241  MVEFLHMDDCDKAKTFSCMSAATSQMVLDHVPPNVATPFLTGGVGGFSGNLKLCIPLRDG 300
            MVEFLHMDDCDK KTFSCMSAATSQMVLDHVP NVATPFLTGGVGGFSGNLKLCIPLRDG
Sbjct: 241  MVEFLHMDDCDKEKTFSCMSAATSQMVLDHVPSNVATPFLTGGVGGFSGNLKLCIPLRDG 300

Query: 301  SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNGDRYINNKVNESDYFEQAS 360
            SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKN D  INNKVNESD FE+AS
Sbjct: 301  SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNSDGCINNKVNESDDFERAS 360

Query: 361  HRHSSALASAEMTPDETSPHCGGMLPGSHLISNWVPLSVKSREKEKVEEFDFGASVDQFF 420
            H HSS LASAEMTPDETSPHCGGMLPGSHLISNWVPLSVKS EKEKVEEFDFGASVDQFF
Sbjct: 361  HSHSSTLASAEMTPDETSPHCGGMLPGSHLISNWVPLSVKSGEKEKVEEFDFGASVDQFF 420

Query: 421  ECLDEIRSTQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV 480
            ECLDEIRS+QSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV
Sbjct: 421  ECLDEIRSSQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV 480

Query: 481  ISFHDDNKFHFTDAEKVQIKADSEVHFVAAKFSDIHLLMQ-------------------- 540
            ISF DDNK+HF D EKV+IKADSEVHFVAAKFSD+HLLMQ                    
Sbjct: 481  ISFRDDNKYHFADTEKVEIKADSEVHFVAAKFSDVHLLMQVSTQRTRFHGTIKHVEIADY 540

Query: 541  ---------TDFRNSNGDFQTILMKRLQVDVLGALPPFEFSAEDPDLVESNGSFNMDLPC 600
                     TDF NSNGDFQTILMKRLQVDVLGALPPF+FSA DPDL+ESN SFNM+LPC
Sbjct: 541  SNCNSYASKTDFCNSNGDFQTILMKRLQVDVLGALPPFDFSAGDPDLMESNSSFNMELPC 600

Query: 601  ENKDNVTKITLLETYGITSSQLNTTSSSNDNSTMTKSFSLNLPPFVFWVNYTLVNMLLDL 660
            ENKDNV KITLLETY ITSSQLN TS SNDNSTM+KSFSLNLPPFVFWVNYTLVNMLLDL
Sbjct: 601  ENKDNVAKITLLETYDITSSQLNMTSGSNDNSTMSKSFSLNLPPFVFWVNYTLVNMLLDL 660

Query: 661  LKDVANCMPGDNNHMCFKENYTSDHEDAKSSSNQVTALTLWAILPGIDI----------- 720
            L+DVANCMPGDNNHMCFKE YTSDHEDAKSSSNQVTAL+  ++   + I           
Sbjct: 661  LEDVANCMPGDNNHMCFKEKYTSDHEDAKSSSNQVTALSFSSMQGNVIISNARVIFCFPL 720

Query: 721  ---------------IALDFYSPPITKEETTHRGNLALQKSYLSQKNALHFRFGSLGVFL 780
                           IALDFYSPPITKEETTHRGNLALQKSYLSQKNALHF FGS+GVFL
Sbjct: 721  ESDKDFMGYSTWDRFIALDFYSPPITKEETTHRGNLALQKSYLSQKNALHFGFGSVGVFL 780

Query: 781  VTFEEDIKQSSTCNLQRKKFSAHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLAS 840
            VT EEDIKQSSTCNLQRKKFSAHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSL  
Sbjct: 781  VTSEEDIKQSSTCNLQRKKFSAHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLVC 840

Query: 841  LEESKSRCKFIGKDYEFASVANMKDMED------------STSVLHVSFSLVRINVGTVQ 900
            LEESKS CKFIGKDYEFASVANMKDME+            STSVLHVSF LVRINVGT Q
Sbjct: 841  LEESKSSCKFIGKDYEFASVANMKDMEESNLQTRQEMILSSTSVLHVSFPLVRINVGTAQ 900

Query: 901  YEAFHCLLDQLVKGLSSETCDVVDVTKGVACQTSILVDCNSLEIVIRPDSNESTKCSLQR 960
            Y+AFHCLLDQL+KGLS ETCDVVDVTKGVACQTSI+VDCNSLEIVIRPD NESTKCSLQR
Sbjct: 901  YKAFHCLLDQLIKGLSRETCDVVDVTKGVACQTSIVVDCNSLEIVIRPDLNESTKCSLQR 960

Query: 961  ELPGSWYHLRLEIQNFELISVTDLGGIKGGNFFWLAHGEGKLLGFISEDPDQEFLLISCS 1020
            ELPGSWYHLRLEIQNFELISV+DLGGIKG NFFWLAHGEGKLLGFISEDPDQEFLLISCS
Sbjct: 961  ELPGSWYHLRLEIQNFELISVSDLGGIKGANFFWLAHGEGKLLGFISEDPDQEFLLISCS 1020

Query: 1021 NSNMKRGDGEGSNALSSRLAGCDIVHSWDPESLQGFSSVTIRCATIFAIGGRLDWLDVIY 1080
            NSNMKRGDGEGSNALSSRLAGCDIVH WDPESLQGFSSVTIRCATI AIGGRLDWLDVIY
Sbjct: 1021 NSNMKRGDGEGSNALSSRLAGCDIVHLWDPESLQGFSSVTIRCATILAIGGRLDWLDVIY 1080

Query: 1081 SFFFLSSPPVEPEDDKIMTRENPKKSSGSYFFLNFVDVGLSYHPYLKNLLVQSGLSQTES 1140
            SFFFLSSPPVEP  DKIMTRENPK SSGS+FFLNF+DVGL+YHPYLKNLL+ SGLSQTES
Sbjct: 1081 SFFFLSSPPVEP-GDKIMTRENPKNSSGSHFFLNFIDVGLNYHPYLKNLLINSGLSQTES 1140

Query: 1141 SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHVFD 1200
            SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEH+ D
Sbjct: 1141 SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHLVD 1200

Query: 1201 AYSVEDLRKVGYVKVARETCIEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA 1260
            AYSVEDLRKVGYVKVARET IEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA
Sbjct: 1201 AYSVEDLRKVGYVKVARETFIEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA 1260

Query: 1261 AQLQQLFAPDLEESIVHLQTRWNNVQQGQERKEIDAGSSSPPCHNLSVKQSEVGLMDEIC 1320
            AQLQQLFAPDLEESIVHLQTRWNNVQQGQERKE+DA SSSPPCHNLSV QSEVGLMDEIC
Sbjct: 1261 AQLQQLFAPDLEESIVHLQTRWNNVQQGQERKEVDAESSSPPCHNLSVNQSEVGLMDEIC 1320

Query: 1321 EDAFLLNKNDSK-------------NVIIASQNVVCSSNSEVRETSSPAHSFMGSDPDGQ 1380
            EDAFLLNKN S+             N ++ ++  VCSSNSEV ETSSPA+SFMGSDPDGQ
Sbjct: 1321 EDAFLLNKNHSRECDYSKSKCSFSPNEVLHAE--VCSSNSEVCETSSPANSFMGSDPDGQ 1380

Query: 1381 TSFIQYRQFPEIIEGYCLSNLRSLPDLTIGRELHPEICNGRNSGSIDTGGRRSGWYGDLP 1440
            TSFIQYRQFPEIIEGYCLSNLRSLPDLT GRELHP+ICN RNSGSIDTGGRRSGWYGDLP
Sbjct: 1381 TSFIQYRQFPEIIEGYCLSNLRSLPDLTTGRELHPDICNARNSGSIDTGGRRSGWYGDLP 1440

Query: 1441 IKILENHVSDVSKVEHSVTNDLCSSESKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVS 1500
            IKILENHVSDVSKVEH VTNDLCS+ESKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVS
Sbjct: 1441 IKILENHVSDVSKVEHLVTNDLCSTESKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVS 1500

Query: 1501 RENGDPPMGMVKRDQHTCLELALSSMQ--------------------------------- 1560
            RENGDPPMGMVKRDQHTCLEL LSSMQ                                 
Sbjct: 1501 RENGDPPMGMVKRDQHTCLELVLSSMQVQYDIFPVGGMCISRLSLSVQDFHLYDSSVDAP 1560

Query: 1561 ---VLGYYNSKNHPRKSSSKAFKLDLEAIRPDPL-------------------------- 1620
               VLGYYNSKNHPRKSSSKAFKLDLEAIRPDPL                          
Sbjct: 1561 WKLVLGYYNSKNHPRKSSSKAFKLDLEAIRPDPLIPLEEYRLCIGILPILLHLHQCQLDF 1620

Query: 1621 ----------SLLRNTGQPPDSDASKTISSTKSQDGLTLAEEALLPYFQKFDIQPIVVRV 1680
                      S  R++GQP DSD SKTIS+TKS DGLTLAEEALLPYFQKFD+ P+VVRV
Sbjct: 1621 FVNFFGERSSSRNRSSGQPLDSDGSKTISTTKSHDGLTLAEEALLPYFQKFDMLPVVVRV 1680

Query: 1681 DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS 1740
            DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS
Sbjct: 1681 DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS 1740

Query: 1741 HNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQRGTIAFLRSISLEA 1796
            HNQIRKILEGLPAVRSLVAVGSGASKLV+SPVESYKKDRRILKGMQRGTIAFLRSISLEA
Sbjct: 1741 HNQIRKILEGLPAVRSLVAVGSGASKLVTSPVESYKKDRRILKGMQRGTIAFLRSISLEA 1800

BLAST of PI0000519 vs. ExPASy TrEMBL
Match: A0A5A7T6C1 (Autophagy-related protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold379G00080 PE=3 SV=1)

HSP 1 Score: 3234.5 bits (8385), Expect = 0.0e+00
Identity = 1674/1947 (85.98%), Postives = 1718/1947 (88.24%), Query Frame = 0

Query: 1    MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL 60
            MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL
Sbjct: 1    MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL 60

Query: 61   NVDFLNEKVGTAATVIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHMDCCG 120
            N       VGTAA+V FKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHM+ CG
Sbjct: 61   N-------VGTAASVTFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHMNGCG 120

Query: 121  AFSGGHNKNHHESRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA 180
            AFSGGH+KNHH SRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA
Sbjct: 121  AFSGGHSKNHHGSRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA 180

Query: 181  FDSFYDENKNRTEFDTTLVLRIADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA 240
            FDSFYDENKNRTEFDTTLVLR+ADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA
Sbjct: 181  FDSFYDENKNRTEFDTTLVLRVADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA 240

Query: 241  MVEFLHMDDCDKAKTFSCMSAATSQMVLDHVPPNVATPFLTGGVGGFSGNLKLCIPLRDG 300
            MVEFLHMDDCDK KTFSCMSAATSQMVLDHVP NVATPFLTGGVGGFSGNLKLCIPLRDG
Sbjct: 241  MVEFLHMDDCDKEKTFSCMSAATSQMVLDHVPSNVATPFLTGGVGGFSGNLKLCIPLRDG 300

Query: 301  SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNGDRYINNKVNESDYFEQAS 360
            SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKN D  INNKVNESD FE+AS
Sbjct: 301  SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNSDGCINNKVNESDDFERAS 360

Query: 361  HRHSSALASAEMTPDETSPHCGGMLPGSHLISNWVPLSVKSREKEKVEEFDFGASVDQFF 420
            H HSS LASAEMTPDETSPHCGGMLPGSHLISNWVPLSVKS EKEKVEEFDFGASVDQFF
Sbjct: 361  HSHSSTLASAEMTPDETSPHCGGMLPGSHLISNWVPLSVKSGEKEKVEEFDFGASVDQFF 420

Query: 421  ECLDEIRSTQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV 480
            ECLDEIRS+QSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV
Sbjct: 421  ECLDEIRSSQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV 480

Query: 481  ISFHDDNKFHFTDAEKVQIKADSEVHFVAAKFSDIHLLMQ-------------------- 540
            ISF DDNK+HF D EKV+IKADSEVHFVAAKFSD+HLLMQ                    
Sbjct: 481  ISFRDDNKYHFADTEKVEIKADSEVHFVAAKFSDVHLLMQVSTQRTRFHGTIKHVEIADY 540

Query: 541  ---------TDFRNSNGDFQTILMKRLQVDVLGALPPFEFSAEDPDLVESNGSFNMDLPC 600
                     TDF NSNGDFQTILMKRLQVDVLGALPPF+FSA DPDL+ESN SFNM+LPC
Sbjct: 541  SNCNSYASKTDFCNSNGDFQTILMKRLQVDVLGALPPFDFSAGDPDLMESNSSFNMELPC 600

Query: 601  ENKDNVTKITLLETYGITSSQLNTTSSSNDNSTMTKSFSLNLPPFVFWVNYTLVNMLLDL 660
            ENKDNV KITLLETY ITSSQLN TS SNDNSTM+KSFSLNLPPFVFWVNYTLVNMLLDL
Sbjct: 601  ENKDNVAKITLLETYDITSSQLNMTSGSNDNSTMSKSFSLNLPPFVFWVNYTLVNMLLDL 660

Query: 661  LKDVANCMPGDNNHMCFKENYTSDHEDAKSSSNQVTALTLWAILPGIDI----------- 720
            L+DVANCMPGDNNHMCFKE YTSDHEDAKSSSNQVTAL+  ++   + I           
Sbjct: 661  LEDVANCMPGDNNHMCFKEKYTSDHEDAKSSSNQVTALSFSSMQGNVIISNARVIFCFPL 720

Query: 721  ---------------IALDFYSPPITKEETTHRGNLALQKSYLSQKNALHFRFGSLGVFL 780
                           IALDFYSPPITKEETTHRGNLALQKSYLSQKNALHF FGS+GVFL
Sbjct: 721  ESDKDFMGYSTWDRFIALDFYSPPITKEETTHRGNLALQKSYLSQKNALHFGFGSVGVFL 780

Query: 781  VTFEEDIKQSSTCNLQRKKFSAHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLAS 840
            VT EEDIKQSSTCNLQRKKFSAHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSL  
Sbjct: 781  VTSEEDIKQSSTCNLQRKKFSAHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLVC 840

Query: 841  LEESKSRCKFIGKDYEFASVANMKDMED------------STSVLHVSFSLVRINVGTVQ 900
            LEESKS CKFIGKDYEFASVANMKDME+            STSVLHVSF LVRINVGT Q
Sbjct: 841  LEESKSSCKFIGKDYEFASVANMKDMEESNLQTRQEMILSSTSVLHVSFPLVRINVGTAQ 900

Query: 901  YEAFHCLLDQLVKGLSSETCDVVDVTKGVACQTSILVDCNSLEIVIRPDSNESTKCSLQR 960
            Y+AFHCLLDQL+KGLS ETCDVVDVTKGVACQTSI+VDCNSLEIVIRPD NESTKCSLQR
Sbjct: 901  YKAFHCLLDQLIKGLSRETCDVVDVTKGVACQTSIVVDCNSLEIVIRPDLNESTKCSLQR 960

Query: 961  ELPGSWYHLRLEIQNFELISVTDLGGIKGGNFFWLAHGEGKLLGFISEDPDQEFLLISCS 1020
            ELPGSWYHLRLEIQNFELISV+DLGGIKG NFFWLAHGEGKLLGFISEDPDQEFLLISCS
Sbjct: 961  ELPGSWYHLRLEIQNFELISVSDLGGIKGANFFWLAHGEGKLLGFISEDPDQEFLLISCS 1020

Query: 1021 NSNMKRGDGEGSNALSSRLAGCDIVHSWDPESLQGFSSVTIRCATIFAIGGRLDWLDVIY 1080
            NSNMKRGDGEGSNALSSRLAGCDIVH WDPESLQGFSSVTIRCATI AIGGRLDWLDVIY
Sbjct: 1021 NSNMKRGDGEGSNALSSRLAGCDIVHLWDPESLQGFSSVTIRCATILAIGGRLDWLDVIY 1080

Query: 1081 SFFFLSSPPVEPEDDKIMTRENPKKSSGSYFFLNFVDVGLSYHPYLKNLLVQSGLSQTES 1140
            SFFFLSSPPVEP  DKIMTRENPK SSGS+FFLNF+DVGL+YHPYLKNLL+ SGLSQTES
Sbjct: 1081 SFFFLSSPPVEP-GDKIMTRENPKNSSGSHFFLNFIDVGLNYHPYLKNLLINSGLSQTES 1140

Query: 1141 SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHVFD 1200
            SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEH+ D
Sbjct: 1141 SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHLVD 1200

Query: 1201 AYSVEDLRKVGYVKVARETCIEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA 1260
            AYSVEDLRKVGYVKVARET IEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA
Sbjct: 1201 AYSVEDLRKVGYVKVARETFIEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA 1260

Query: 1261 AQLQQLFAPDLEESIVHLQTRWNNVQQGQERKEIDAGSSSPPCHNLSVKQSEVGLMDEIC 1320
            AQLQQLFAPDLEESIVHLQTRWNNVQQGQERKE+DA SSSPPCHNLSV QSEVGLMDEIC
Sbjct: 1261 AQLQQLFAPDLEESIVHLQTRWNNVQQGQERKEVDAESSSPPCHNLSVNQSEVGLMDEIC 1320

Query: 1321 EDAFLLNKNDSK-------------NVIIASQNVVCSSNSEVRETSSPAHSFMGSDPDGQ 1380
            EDAFLLNKN S+             N ++ ++  VCSSNSEV ETSSPA+SFMGSDPDGQ
Sbjct: 1321 EDAFLLNKNHSRECDYSKSKCSFSPNEVLHAE--VCSSNSEVCETSSPANSFMGSDPDGQ 1380

Query: 1381 TSFIQYRQFPEIIEGYCLSNLRSLPDLTIGRELHPEICNGRNSGSIDTGGRRSGWYGDLP 1440
            TSFIQYRQFPEIIEGYCLSNLRSLPDLT GRELHP+ICN RNSGSIDTGGRRSGWYGDLP
Sbjct: 1381 TSFIQYRQFPEIIEGYCLSNLRSLPDLTTGRELHPDICNARNSGSIDTGGRRSGWYGDLP 1440

Query: 1441 IKILENHVSDVSKVEHSVTNDLCSSESKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVS 1500
            IKILENHVSDVSKVEH VTNDLCS+ESKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVS
Sbjct: 1441 IKILENHVSDVSKVEHLVTNDLCSTESKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVS 1500

Query: 1501 RENGDPPMGMVKRDQHTCLELALSSMQ--------------------------------- 1560
            RENGDPPMGMVKRDQHTCLEL LSSMQ                                 
Sbjct: 1501 RENGDPPMGMVKRDQHTCLELVLSSMQVQYDIFPVGGMCISRLSLSVQDFHLYDSSVDAP 1560

Query: 1561 ---VLGYYNSKNHPRKSSSKAFKLDLEAIRPDPL-------------------------- 1620
               VLGYYNSKNHPRKSSSKAFKLDLEAIRPDPL                          
Sbjct: 1561 WKLVLGYYNSKNHPRKSSSKAFKLDLEAIRPDPLIPLEEYRLCIGILPILLHLHQCQLDF 1620

Query: 1621 ----------SLLRNTGQPPDSDASKTISSTKSQDGLTLAEEALLPYFQKFDIQPIVVRV 1680
                      S  R++GQP DSD SKTIS+TKS DGLTLAEEALLPYFQKFD+ P+VVRV
Sbjct: 1621 FVNFFGERSSSRNRSSGQPLDSDGSKTISTTKSHDGLTLAEEALLPYFQKFDMLPVVVRV 1680

Query: 1681 DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS 1740
            DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS
Sbjct: 1681 DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS 1740

Query: 1741 HNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQRGTIAFLRSISLEA 1796
            HNQIRKILEGLPAVRSLVAVGSGASKLV+SPVESYKKDRRILKGMQRGTIAFLRSISLEA
Sbjct: 1741 HNQIRKILEGLPAVRSLVAVGSGASKLVTSPVESYKKDRRILKGMQRGTIAFLRSISLEA 1800

BLAST of PI0000519 vs. ExPASy TrEMBL
Match: A0A5D3CQA0 (Autophagy-related protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold832G001750 PE=3 SV=1)

HSP 1 Score: 3087.0 bits (8002), Expect = 0.0e+00
Identity = 1595/1861 (85.71%), Postives = 1638/1861 (88.02%), Query Frame = 0

Query: 89   MPWTSRGCEVEINGLELVLSPCLKNVHMDCCGAFSGGHNKNHHESRKSEHDVVKNAAKST 148
            MPWTSRGCEVEINGLELVLSPCLKNVHM+ CGAFSGGH+KNHH SRKSEHDVVKNAAKST
Sbjct: 1    MPWTSRGCEVEINGLELVLSPCLKNVHMNGCGAFSGGHSKNHHGSRKSEHDVVKNAAKST 60

Query: 149  YGDIHEGVKTVAKMVKGLLASFHLKIINLIVAFDSFYDENKNRTEFDTTLVLRIADVECG 208
            YGDIHEGVKTVAKMVKGLLASFHLKIINLIVAFDSFYDENKNRTEFDTTLVLR+ADVECG
Sbjct: 61   YGDIHEGVKTVAKMVKGLLASFHLKIINLIVAFDSFYDENKNRTEFDTTLVLRVADVECG 120

Query: 209  TCVTEDGKLGMDAVESFLGISQLNNFVKFQGAMVEFLHMDDCDKAKTFSCMSAATSQMVL 268
            TCVTEDGKLGMDAVESFLGISQLNNFVKFQGAMVEFLHMDDCDK KTFSCMSAATSQMVL
Sbjct: 121  TCVTEDGKLGMDAVESFLGISQLNNFVKFQGAMVEFLHMDDCDKEKTFSCMSAATSQMVL 180

Query: 269  DHVPPNVATPFLTGGVGGFSGNLKLCIPLRDGSLDIYRVDGDLSFDPVQLKLQPRTIKCL 328
            DHVP NVATPFLTGGVGGFSGNLKLCIPLRDGSLDIYRVDGDLSFDPVQLKLQPRTIKCL
Sbjct: 181  DHVPSNVATPFLTGGVGGFSGNLKLCIPLRDGSLDIYRVDGDLSFDPVQLKLQPRTIKCL 240

Query: 329  LTLSEAYWNSDKNGDRYINNKVNESDYFEQASHRHSSALASAEMTPDETSPHCGGMLPGS 388
            LTLSEAYWNSDKN D  INNKVNESD FE+ASH HSS LASAEMTPDETSPHCGGMLPGS
Sbjct: 241  LTLSEAYWNSDKNSDGCINNKVNESDDFERASHSHSSTLASAEMTPDETSPHCGGMLPGS 300

Query: 389  HLISNWVPLSVKSREKEKVEEFDFGASVDQFFECLDEIRSTQSALGSSGMWNSVFSAITA 448
            HLISNWVPLSVKS EKEKVEEFDFGASVDQFFECLDEIRS+QSALGSSGMWNSVFSAITA
Sbjct: 301  HLISNWVPLSVKSGEKEKVEEFDFGASVDQFFECLDEIRSSQSALGSSGMWNSVFSAITA 360

Query: 449  ASSLASGSLHVPS--ELQPVETNLRATISGISIVISFHDDNKFHFTDAEKVQIKADSEVH 508
            ASSLASGSLHVPS  ELQPVETNLRATISGISIVISF DDNK+HF D EKV+IKADSEVH
Sbjct: 361  ASSLASGSLHVPSDTELQPVETNLRATISGISIVISFRDDNKYHFADTEKVEIKADSEVH 420

Query: 509  FVAAKFSDIHLLMQ-----------------------------TDFRNSNGDFQTILMKR 568
            FVAAKFSD+HLLMQ                             TDF NSNGDFQTILMKR
Sbjct: 421  FVAAKFSDVHLLMQVSTQRTRFHGTIKHVEIADYSNCNSYASKTDFCNSNGDFQTILMKR 480

Query: 569  LQVDVLGALPPFEFSAEDPDLVESNGSFNMDLPCENKDNVTKITLLETYGITSSQLNTTS 628
            LQVDVLGALPPF+FSA DPDL+ESN SFNM+LPCENKDNV KITLLETY ITSSQLN TS
Sbjct: 481  LQVDVLGALPPFDFSAGDPDLMESNSSFNMELPCENKDNVAKITLLETYDITSSQLNMTS 540

Query: 629  SSNDNSTMTKSFSLNLPPFVFWVNYTLVNMLLDLLKDVANCMPGDNNHMCFKENYTSDHE 688
             SNDNSTM+KSFSLNLPPFVFWVNYTLVNMLLDLL+DVANCMPGDNNHMCFKE YTSDHE
Sbjct: 541  GSNDNSTMSKSFSLNLPPFVFWVNYTLVNMLLDLLEDVANCMPGDNNHMCFKEKYTSDHE 600

Query: 689  DAKSSSNQVTALTLWAILPGIDI--------------------------IALDFYSPPIT 748
            DAKSSSNQVTAL+  ++   + I                          IALDFYSPPIT
Sbjct: 601  DAKSSSNQVTALSFSSMQGNVIISNARVIFCFPLESDKDFMGYSTWDRFIALDFYSPPIT 660

Query: 749  KEETTHRGNLALQKSYLSQKNALHFRFGSLGVFLVTFEEDIKQSSTCNLQRKKFSAHNIL 808
            KEETTHRGNLALQKSYLSQKNALHF FGS+GVFLVT EEDIKQSSTCNLQRKKFSAHNIL
Sbjct: 661  KEETTHRGNLALQKSYLSQKNALHFGFGSVGVFLVTSEEDIKQSSTCNLQRKKFSAHNIL 720

Query: 809  SASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLASLEESKSRCKFIGKDYEFASVANMKDM 868
            SASNRTNGSPLTLFWQEGHVTGPWIAKKAKSL  LEESKS CKFIGKDYEFASVANMKDM
Sbjct: 721  SASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLVCLEESKSSCKFIGKDYEFASVANMKDM 780

Query: 869  ED------------STSVLHVSFSLVRINVGTVQYEAFHCLLDQLVKGLSSETCDVVDVT 928
            E+            STSVLHVSF LVRINVGT QY+AFHCLLDQL+KGLS ETCDVVDVT
Sbjct: 781  EESNLQTRQEMILSSTSVLHVSFPLVRINVGTAQYKAFHCLLDQLIKGLSRETCDVVDVT 840

Query: 929  KGVACQTSILVDCNSLEIVIRPDSNESTKCSLQRELPGSWYHLRLEIQNFELISVTDLGG 988
            KGVACQTSI+VDCNSLEIVIRPD NESTKCSLQRELPGSWYHLRLEIQNFELISV+DLGG
Sbjct: 841  KGVACQTSIVVDCNSLEIVIRPDLNESTKCSLQRELPGSWYHLRLEIQNFELISVSDLGG 900

Query: 989  IKGGNFFWLAHGEGKLLGFISEDPDQEFLLISCSNSNMKRGDGEGSNALSSRLAGCDIVH 1048
            IKG NFFWLAHGEGKLLGFISEDPDQEFLLISCSNSNMKRGDGEGSNALSSRLAGCDIVH
Sbjct: 901  IKGANFFWLAHGEGKLLGFISEDPDQEFLLISCSNSNMKRGDGEGSNALSSRLAGCDIVH 960

Query: 1049 SWDPESLQGFSSVTIRCATIFAIGGRLDWLDVIYSFFFLSSPPVEPEDDKIMTRENPKKS 1108
             WDPESLQGFSSVTIRCATI AIGGRLDWLDVIYSFFFLSSPPVEP  DKIMTRENPK S
Sbjct: 961  LWDPESLQGFSSVTIRCATILAIGGRLDWLDVIYSFFFLSSPPVEP-GDKIMTRENPKNS 1020

Query: 1109 SGSYFFLNFVDVGLSYHPYLKNLLVQSGLSQTESSSSTFKQELDDDYVACLLAASSVTLS 1168
            SGS+FFLNF+DVGL+YHPYLKNLL+ SGLSQTESSSSTFKQELDDDYVACLLAASSVTLS
Sbjct: 1021 SGSHFFLNFIDVGLNYHPYLKNLLINSGLSQTESSSSTFKQELDDDYVACLLAASSVTLS 1080

Query: 1169 SSSVADVVEDNYRITVQDFGLLLCSVSDYEHVFDAYSVEDLRKVGYVKVARETCIEAILR 1228
            SSSVADVVEDNYRITVQDFGLLLCSVSDYEH+ DAYSVEDLRKVGYVKVARET IEAILR
Sbjct: 1081 SSSVADVVEDNYRITVQDFGLLLCSVSDYEHLVDAYSVEDLRKVGYVKVARETFIEAILR 1140

Query: 1229 TNCNNGLKWELECGKAHISVETCHDTASGLARLAAQLQQLFAPDLEESIVHLQTRWNNVQ 1288
            TNCNNGLKWELECGKAHISVETCHDTASGLARLAAQLQQLFAPDLEESIVHLQTRWNNVQ
Sbjct: 1141 TNCNNGLKWELECGKAHISVETCHDTASGLARLAAQLQQLFAPDLEESIVHLQTRWNNVQ 1200

Query: 1289 QGQERKEIDAGSSSPPCHNLSVKQSEVGLMDEICEDAFLLNKNDSK-------------N 1348
            QGQERKE+DA SSSPPCHNLSV QSEVGLMDEICEDAFLLNKN S+             N
Sbjct: 1201 QGQERKEVDAESSSPPCHNLSVNQSEVGLMDEICEDAFLLNKNHSRECDYSKSKCSFSPN 1260

Query: 1349 VIIASQNVVCSSNSEVRETSSPAHSFMGSDPDGQTSFIQYRQFPEIIEGYCLSNLRSLPD 1408
             ++ ++  VCSSNSEV ETSSPA+SFMGSDPDGQTSFIQYRQFPEIIEGYCLSNLRSLPD
Sbjct: 1261 EVLHAE--VCSSNSEVCETSSPANSFMGSDPDGQTSFIQYRQFPEIIEGYCLSNLRSLPD 1320

Query: 1409 LTIGRELHPEICNGRNSGSIDTGGRRSGWYGDLPIKILENHVSDVSKVEHSVTNDLCSSE 1468
            LT GRELHP+ICN RNSGSIDTGGRRSGWYGDLPIKILENHVSDVSKVEH VTNDLCS+E
Sbjct: 1321 LTTGRELHPDICNARNSGSIDTGGRRSGWYGDLPIKILENHVSDVSKVEHLVTNDLCSTE 1380

Query: 1469 SKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVSRENGDPPMGMVKRDQHTCLELALSSM 1528
            SKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVSRENGDPPMGMVKRDQHTCLEL LSSM
Sbjct: 1381 SKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVSRENGDPPMGMVKRDQHTCLELVLSSM 1440

Query: 1529 Q------------------------------------VLGYYNSKNHPRKSSSKAFKLDL 1588
            Q                                    VLGYYNSKNHPRKSSSKAFKLDL
Sbjct: 1441 QVQYDIFPVGGMCISRLSLSVQDFHLYDSSVDAPWKLVLGYYNSKNHPRKSSSKAFKLDL 1500

Query: 1589 EAIRPDPL------------------------------------SLLRNTGQPPDSDASK 1648
            EAIRPDPL                                    S  R++GQP DSD SK
Sbjct: 1501 EAIRPDPLIPLEEYRLCIGILPILLHLHQCQLDFFVNFFGERSSSRNRSSGQPLDSDGSK 1560

Query: 1649 TISSTKSQDGLTLAEEALLPYFQKFDIQPIVVRVDYSPSRVDLAALRGGKYVELVNLVPW 1708
            TIS+TKS DGLTLAEEALLPYFQKFD+ P+VVRVDYSPSRVDLAALRGGKYVELVNLVPW
Sbjct: 1561 TISTTKSHDGLTLAEEALLPYFQKFDMLPVVVRVDYSPSRVDLAALRGGKYVELVNLVPW 1620

Query: 1709 KGVELHLKHVQAVGVYGWGSVCETVVGEWLEDISHNQIRKILEGLPAVRSLVAVGSGASK 1768
            KGVELHLKHVQAVGVYGWGSVCETVVGEWLEDISHNQIRKILEGLPAVRSLVAVGSGASK
Sbjct: 1621 KGVELHLKHVQAVGVYGWGSVCETVVGEWLEDISHNQIRKILEGLPAVRSLVAVGSGASK 1680

Query: 1769 LVSSPVESYKKDRRILKGMQRGTIAFLRSISLEAVGLGVHLAAGAHDILLQAEYILTSIP 1796
            LV+SPVESYKKDRRILKGMQRGTIAFLRSISLEAVGLGVHLAAGAHDILLQAEYILTSIP
Sbjct: 1681 LVTSPVESYKKDRRILKGMQRGTIAFLRSISLEAVGLGVHLAAGAHDILLQAEYILTSIP 1740

BLAST of PI0000519 vs. ExPASy TrEMBL
Match: A0A6J1FQW6 (Autophagy-related protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111447387 PE=3 SV=1)

HSP 1 Score: 2941.4 bits (7624), Expect = 0.0e+00
Identity = 1536/1947 (78.89%), Postives = 1625/1947 (83.46%), Query Frame = 0

Query: 1    MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL 60
            MF WNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL
Sbjct: 1    MFTWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL 60

Query: 61   NVDFLNEKVGTAATVIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHMDCCG 120
            NVDFLNEKVGTAA V FKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCL N  MDCCG
Sbjct: 61   NVDFLNEKVGTAALVTFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLSNDRMDCCG 120

Query: 121  AFSGGHNKNHHESRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA 180
              SGGH KNHHESRKSEH V K+A KSTYGDIHEGVKTVAKMVKGLLASFH+KIINLIVA
Sbjct: 121  TSSGGH-KNHHESRKSEHFVAKSAVKSTYGDIHEGVKTVAKMVKGLLASFHVKIINLIVA 180

Query: 181  FDSFYDENKNRTEFDTTLVLRIADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA 240
            FDSFYDE+KN+T  DTTLVLRI+DVECGTCVTEDGKL MDA ESFLGISQL+NFVKFQGA
Sbjct: 181  FDSFYDESKNKTGLDTTLVLRISDVECGTCVTEDGKLDMDAAESFLGISQLSNFVKFQGA 240

Query: 241  MVEFLHMDDCDKAKTFSCMSAATS-QMVLDHVPPNVATPFLTGGVGGFSGNLKLCIPLRD 300
            MVEFLHMDDCD  K+ SCMSA TS +M LDHVP NV  P LTGGVGGFSGNLKL IPLRD
Sbjct: 241  MVEFLHMDDCDNPKSISCMSAGTSAEMALDHVPSNVTIPVLTGGVGGFSGNLKLRIPLRD 300

Query: 301  GSLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNGDRY--INNKVNESDYFE 360
            GSLDIYRVDGDL  DPVQ+KLQPRTIKC L LSEAYWNSDKNGD +   +NK+NES YF+
Sbjct: 301  GSLDIYRVDGDLCIDPVQVKLQPRTIKCFLVLSEAYWNSDKNGDGHGCKHNKLNESGYFD 360

Query: 361  QASHRHSSALASAEMTPDET-SPHCGGMLPGSHLISNWVPLSVKSREKEKV-EEFDFGAS 420
            +ASH HS  LASAE TPDET SPHCGG+LPGSHLISNWVP SVK REKEKV EEFDFGAS
Sbjct: 361  RASHDHSFTLASAERTPDETSSPHCGGILPGSHLISNWVPSSVKHREKEKVEEEFDFGAS 420

Query: 421  VDQFFECLDEIRSTQSALGSSGMWNSVFSAITAASSLASGSLHVP--SELQPVETNLRAT 480
            VDQFFECLDEIRS+QSALGSSG+WNSVFSAITAASSLASGSL +P  +ELQPVETNLRAT
Sbjct: 421  VDQFFECLDEIRSSQSALGSSGVWNSVFSAITAASSLASGSLPIPCDTELQPVETNLRAT 480

Query: 481  ISGISIVISFHDDNKFHFTDAEKVQIKADSEVHFVAAKFSDIHLLMQ------------- 540
            ISGIS+VISFHDDN++HFTD EKVQIK DSEVHFVAAKFSDIHLLMQ             
Sbjct: 481  ISGISVVISFHDDNQYHFTDPEKVQIKTDSEVHFVAAKFSDIHLLMQVSQKTSFQGTIKH 540

Query: 541  ---------------TDFRNSNGDFQTILMKRLQVDVLGALPPFEFSAEDPDLVESNGSF 600
                           +DF NSN DFQT+L K LQVDV+GALPPF FSAEDP+ VESN SF
Sbjct: 541  IEVADYLNCKNYASKSDFHNSNSDFQTLLTKHLQVDVIGALPPFNFSAEDPNNVESNSSF 600

Query: 601  NMDLPCENKDNVTKITLLETYGITSSQLNT-TSSSNDNSTMTKSFSLNLPPFVFWVNYTL 660
            NMD PCENKDN TKITLL+TYGITSSQLN  +SSS DNSTM+KSFSLNLPPF+FWVNYTL
Sbjct: 601  NMDFPCENKDNFTKITLLKTYGITSSQLNVISSSSGDNSTMSKSFSLNLPPFIFWVNYTL 660

Query: 661  VNMLLDLLKDVANCMPGDNNHMCFKENYTSDHEDAKSSSNQVTALTLWAILPGIDI---- 720
            VNMLLDLL+ VANC+PGDNNH CFK+NY SDH++ KS  NQVTAL+  A + G  I    
Sbjct: 661  VNMLLDLLRGVANCIPGDNNHKCFKDNYVSDHKNVKSGCNQVTALSSSASVQGNIIIAKA 720

Query: 721  -----------------------IALDFYSPPITKEETTHRGNLALQKSYLSQKNALHFR 780
                                   IALDFYSPPITKEET H GNLALQK+Y S+KNALHFR
Sbjct: 721  RVIFCFPFESDKDFMGYSSWDQFIALDFYSPPITKEETIHSGNLALQKNYFSEKNALHFR 780

Query: 781  FGSLGVFLVTFEEDIKQSSTCNLQRKKFSAHNILSASNRTNGSPLTLFWQEGHVTGPWIA 840
            FGSLGVFLVT +E+IKQ STCN+QRKKFSAHNILSASNRTN S LT FWQEGHVTGPWIA
Sbjct: 781  FGSLGVFLVTSDEEIKQGSTCNVQRKKFSAHNILSASNRTNASSLTFFWQEGHVTGPWIA 840

Query: 841  KKAKSLASLEESKSRCKFIGKDYEFASVANMKDMED------------STSVLHVSFSLV 900
            KKAKSLASLEES+SRCKFIGKDYEFASVANMKDME+            ST+VLHV   LV
Sbjct: 841  KKAKSLASLEESESRCKFIGKDYEFASVANMKDMEESNLQTRQEMILSSTTVLHVFLPLV 900

Query: 901  RINVGTVQYEAFHCLLDQLVKGLSSETCDVVDVTKG-VACQTSILVDCNSLEIVIRPDSN 960
            RIN G VQY+ F+ LLDQL+KGLSS+TCD VDVTKG V  Q SI+VDC+ +EI IRPD N
Sbjct: 901  RINAGAVQYKVFNYLLDQLIKGLSSDTCDAVDVTKGSVTSQKSIVVDCSCVEISIRPDPN 960

Query: 961  ESTKCSLQRELPGSWYHLRLEIQNFELISVTDLGGIKGGNFFWLAHGEGKLLGFISEDPD 1020
            ES KCSLQRELPGSWYHLRLEIQNFELISV+DLG I+G NFFWL+HGEG+LLGFISEDPD
Sbjct: 961  ESAKCSLQRELPGSWYHLRLEIQNFELISVSDLGSIEGANFFWLSHGEGQLLGFISEDPD 1020

Query: 1021 QEFLLISCSNSNMKRGDGEGSNALSSRLAGCDIVHSWDPESLQGFSSVTIRCATIFAIGG 1080
            QEF LISCSNSNMKRGDGEGSNALSSRLAGCDIVH WDPE LQ FSS+TIRCATI AIGG
Sbjct: 1021 QEFRLISCSNSNMKRGDGEGSNALSSRLAGCDIVHMWDPECLQDFSSITIRCATILAIGG 1080

Query: 1081 RLDWLDVIYSFFFLSSPPVEPEDDKIMTRENPKKSSGSYFFLNFVDVGLSYHPYLKNLLV 1140
            RLDWLDVI SFF L S PVEPE DK+M REN KKSSGS FFLNFVDVGLSY PYLKNLL 
Sbjct: 1081 RLDWLDVICSFFLLPSSPVEPEGDKVMPRENTKKSSGSNFFLNFVDVGLSYQPYLKNLLG 1140

Query: 1141 QSGLSQTESSSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCS 1200
             S LSQTESSSST KQELDDDYVACLLAASSVTLSSSSVAD VEDN+RIT+QD GLLLCS
Sbjct: 1141 NSRLSQTESSSSTIKQELDDDYVACLLAASSVTLSSSSVADTVEDNFRITIQDIGLLLCS 1200

Query: 1201 VSDYEHVFDAYSVEDLRKVGYVKVARETCIEAILRTNCNNGLKWELECGKAHISVETCHD 1260
            VSDYEHVF+AY+VEDLRK G+VKVARET IEAILRTNC+NGLKWELEC KAH+ VETC+D
Sbjct: 1201 VSDYEHVFNAYNVEDLRKGGWVKVARETFIEAILRTNCDNGLKWELECHKAHVFVETCND 1260

Query: 1261 TASGLARLAAQLQQLFAPDLEESIVHLQTRWNNVQQGQERKEIDAGSSSPPCHNLSVKQS 1320
            T +GL RLAAQLQQLFAPDLEESIVHLQTRWNNVQQ QERKEID  SSSPPCHN SVKQS
Sbjct: 1261 TTTGLVRLAAQLQQLFAPDLEESIVHLQTRWNNVQQAQERKEIDNESSSPPCHNPSVKQS 1320

Query: 1321 EVGLMDEICEDAFLLNKNDSKNVIIAS-----QNVVCSSNSEVRETSSPAHSFMGSDPDG 1380
            E GLMDEICEDAFLLNKN ++     S        VC  NSEV ETSSP H F+GS+PDG
Sbjct: 1321 ETGLMDEICEDAFLLNKNQTRECDCFSTTEGLHTEVCRLNSEVHETSSPIHPFIGSEPDG 1380

Query: 1381 QTSFIQYRQFPEIIEGYCLSNLRSLPDLTIGRELHPEICNGRNSGSIDTGGRRSGWYGDL 1440
            QTSFIQYR+FPEIIEGYCLS  R + DLT+G+EL P+ICN RNS SID  GRRSGWYG+L
Sbjct: 1381 QTSFIQYRKFPEIIEGYCLSEFRPVSDLTMGQELPPDICNSRNSSSIDLEGRRSGWYGNL 1440

Query: 1441 PIKILENHVSDVSKVEHSVTNDLCSSESKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQV 1500
            PIKILENH+ D SKVEHSVT+DLCS ESKRLDEVEEA GRVLLNNID+KWRMYAGSDWQV
Sbjct: 1441 PIKILENHILDASKVEHSVTDDLCSIESKRLDEVEEACGRVLLNNIDLKWRMYAGSDWQV 1500

Query: 1501 SRENGDPPMGMVKRDQHTCLELALSSMQ-------------------------------- 1560
            SRENGD PM MVKRDQHTCLELALS+M                                 
Sbjct: 1501 SRENGDRPMSMVKRDQHTCLELALSAMHVQYDVFPMGGMCVSRLSLSVQDFHLYDSSVDA 1560

Query: 1561 ----VLGYYNSKNHPRKSSSKAFKLDLEAIRPDPLSLL---------------------- 1620
                VLGYYNSKNHPRKSSSKAFKLDLEAIRPDPL  L                      
Sbjct: 1561 PWKLVLGYYNSKNHPRKSSSKAFKLDLEAIRPDPLIPLEEYRLRVGFLPMLLHLHQCQLD 1620

Query: 1621 -------------RNTGQPPDSDASKTISSTKSQDGLTLAEEALLPYFQKFDIQPIVVRV 1680
                           +G   DSD SKTIS+TKS  GLTLAEEALLPYFQKFDIQPIVVRV
Sbjct: 1621 FLVNFFGEKSSSGNQSGHSLDSDGSKTISTTKSDAGLTLAEEALLPYFQKFDIQPIVVRV 1680

Query: 1681 DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS 1740
            DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS
Sbjct: 1681 DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS 1740

Query: 1741 HNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQRGTIAFLRSISLEA 1796
            HNQIRKILEGLP VRSLVAVGSGASKL+SSPVESYKKDRRILKGMQRGTIAFLRSISLEA
Sbjct: 1741 HNQIRKILEGLPTVRSLVAVGSGASKLLSSPVESYKKDRRILKGMQRGTIAFLRSISLEA 1800

BLAST of PI0000519 vs. NCBI nr
Match: XP_011651243.1 (autophagy-related protein 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 3278.0 bits (8498), Expect = 0.0e+00
Identity = 1691/1947 (86.85%), Postives = 1731/1947 (88.91%), Query Frame = 0

Query: 1    MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL 60
            MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQL+DLAL
Sbjct: 1    MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLNDLAL 60

Query: 61   NVDFLNEKVGTAATVIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHMDCCG 120
            NVDFLNEKVGTAA+VIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHM+CCG
Sbjct: 61   NVDFLNEKVGTAASVIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHMNCCG 120

Query: 121  AFSGGHNKNHHESRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA 180
            AFSG H+ NHHESRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA
Sbjct: 121  AFSGSHSNNHHESRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA 180

Query: 181  FDSFYDENKNRTEFDTTLVLRIADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA 240
            FDSFYDENKNRTEFDTTLVLRIADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA
Sbjct: 181  FDSFYDENKNRTEFDTTLVLRIADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA 240

Query: 241  MVEFLHMDDCDKAKTFSCMSAATSQMVLDHVPPNVATPFLTGGVGGFSGNLKLCIPLRDG 300
            MVEFLHMDDCDKAKTF CMSAATSQMVLDHVP NVATPFLTGGVGGFSGNLKLCIPLRDG
Sbjct: 241  MVEFLHMDDCDKAKTFPCMSAATSQMVLDHVPSNVATPFLTGGVGGFSGNLKLCIPLRDG 300

Query: 301  SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNGDRYINNKVNESDYFEQAS 360
            SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKN D  INNKVNESDYFE+A 
Sbjct: 301  SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNSDGCINNKVNESDYFERAF 360

Query: 361  HRHSSALASAEMTPDETSPHCGGMLPGSHLISNWVPLSVKSREKEKVEEFDFGASVDQFF 420
            H HSSALASAE TPDETSPHCGGMLPGSHLISNWVPLSVKSREKEKVEEFDFGASVDQFF
Sbjct: 361  HSHSSALASAETTPDETSPHCGGMLPGSHLISNWVPLSVKSREKEKVEEFDFGASVDQFF 420

Query: 421  ECLDEIRSTQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV 480
            ECLDEIRSTQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV
Sbjct: 421  ECLDEIRSTQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV 480

Query: 481  ISFHDDNKFHFTDAEKVQIKADSEVHFVAAKFSDIHLLMQ-------------------- 540
            ISFHDDNK+HFTD EKVQIKADSEVHFVAAKFSD+HLLMQ                    
Sbjct: 481  ISFHDDNKYHFTDTEKVQIKADSEVHFVAAKFSDVHLLMQVSTQRTRFHGTIKHVEIADY 540

Query: 541  ---------TDFRNSNGDFQTILMKRLQVDVLGALPPFEFSAEDPDLVESNGSFNMDLPC 600
                     TDF NSNGDFQTILMKRLQVDVLGALPPF+FSAEDPDLVESN SFNMDLPC
Sbjct: 541  LNCNSYASKTDFCNSNGDFQTILMKRLQVDVLGALPPFDFSAEDPDLVESNSSFNMDLPC 600

Query: 601  ENKDNVTKITLLETYGITSSQLNTTSSSNDNSTMTKSFSLNLPPFVFWVNYTLVNMLLDL 660
            ENKDNV KITLLETYGITSSQLN TSSSNDNSTM+KSFSLNLPPFVFWVNYTLVNMLLDL
Sbjct: 601  ENKDNVAKITLLETYGITSSQLNMTSSSNDNSTMSKSFSLNLPPFVFWVNYTLVNMLLDL 660

Query: 661  LKDVANCMPGDNNHMCFKENYTSDHEDAKSSSNQVTALTLWAILPGIDI----------- 720
            LKDVANCMPGDNNHMCFKENYTSDHEDAKSS NQVTAL+  ++   + I           
Sbjct: 661  LKDVANCMPGDNNHMCFKENYTSDHEDAKSSPNQVTALSFSSMQGNVIISNARVIFCFPL 720

Query: 721  ---------------IALDFYSPPITKEETTHRGNLALQKSYLSQKNALHFRFGSLGVFL 780
                           IALDFY+ PITKEETTHRGNLA+QKSY  QKNALHFRFGS+GVFL
Sbjct: 721  ESDKDFMGYSSWDRFIALDFYASPITKEETTHRGNLAVQKSYQLQKNALHFRFGSVGVFL 780

Query: 781  VTFEEDIKQSSTCNLQRKKFSAHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLAS 840
            VTFEEDIKQSSTCNLQ KKFS HNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLA 
Sbjct: 781  VTFEEDIKQSSTCNLQGKKFSVHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLAC 840

Query: 841  LEESKSRCKFIGKDYEFASVANMKDMED------------STSVLHVSFSLVRINVGTVQ 900
            LEESKS CKFIGKDYEFASVANMKDME+            STSVLHVSF LVRINVGTVQ
Sbjct: 841  LEESKSSCKFIGKDYEFASVANMKDMEESNLQTRQEMILSSTSVLHVSFPLVRINVGTVQ 900

Query: 901  YEAFHCLLDQLVKGLSSETCDVVDVTKGVACQTSILVDCNSLEIVIRPDSNESTKCSLQR 960
            Y+AFHCLLDQL+KGLS ETCDVVDVTKGVACQTSI+VDCNSLEIVIRPD NESTKCSLQR
Sbjct: 901  YKAFHCLLDQLIKGLSRETCDVVDVTKGVACQTSIVVDCNSLEIVIRPDLNESTKCSLQR 960

Query: 961  ELPGSWYHLRLEIQNFELISVTDLGGIKGGNFFWLAHGEGKLLGFISEDPDQEFLLISCS 1020
            ELPGSWYHLRLEIQNFELISV+DLGGIKG NFFWLAHGEGKLLGFISEDPDQEFLLISCS
Sbjct: 961  ELPGSWYHLRLEIQNFELISVSDLGGIKGANFFWLAHGEGKLLGFISEDPDQEFLLISCS 1020

Query: 1021 NSNMKRGDGEGSNALSSRLAGCDIVHSWDPESLQGFSSVTIRCATIFAIGGRLDWLDVIY 1080
            NSNMKRGDGEGSNALSSRLAGCDIVH WDPESLQGFSSVTIRCATI AIGGRLDWLDVIY
Sbjct: 1021 NSNMKRGDGEGSNALSSRLAGCDIVHLWDPESLQGFSSVTIRCATILAIGGRLDWLDVIY 1080

Query: 1081 SFFFLSSPPVEPEDDKIMTRENPKKSSGSYFFLNFVDVGLSYHPYLKNLLVQSGLSQTES 1140
            SFFFLSSPPVEPE DKIMTRENPK SSGS FFLNFVDVGL+YHPYLKNLL++SGLSQ+ES
Sbjct: 1081 SFFFLSSPPVEPEGDKIMTRENPKNSSGSCFFLNFVDVGLNYHPYLKNLLIKSGLSQSES 1140

Query: 1141 SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHVFD 1200
            SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHVFD
Sbjct: 1141 SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHVFD 1200

Query: 1201 AYSVEDLRKVGYVKVARETCIEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA 1260
            AYSVEDLRKVGYVKVARET IEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA
Sbjct: 1201 AYSVEDLRKVGYVKVARETFIEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA 1260

Query: 1261 AQLQQLFAPDLEESIVHLQTRWNNVQQGQERKEIDAGSSSPPCHNLSVKQSEVGLMDEIC 1320
            AQLQQLFAPDLEESIVHLQTRWNN QQGQERKEIDA SSSPPCHNLSV QSEVGLMDEIC
Sbjct: 1261 AQLQQLFAPDLEESIVHLQTRWNNAQQGQERKEIDAESSSPPCHNLSVNQSEVGLMDEIC 1320

Query: 1321 EDAFLLNKNDSK-------------NVIIASQNVVCSSNSEVRETSSPAHSFMGSDPDGQ 1380
            EDAFLLNKN S+             N ++ ++  VCSSNSEV ETSSPAHSFMGSDPDGQ
Sbjct: 1321 EDAFLLNKNHSRECDYSKTKCSFSPNEVLHAE--VCSSNSEVCETSSPAHSFMGSDPDGQ 1380

Query: 1381 TSFIQYRQFPEIIEGYCLSNLRSLPDLTIGRELHPEICNGRNSGSIDTGGRRSGWYGDLP 1440
            TSFIQYRQFPEIIEGYCLSNL SLPDLTIGRELHP+ICNGRNSGSIDTGGRRSGWYGDLP
Sbjct: 1381 TSFIQYRQFPEIIEGYCLSNLCSLPDLTIGRELHPDICNGRNSGSIDTGGRRSGWYGDLP 1440

Query: 1441 IKILENHVSDVSKVEHSVTNDLCSSESKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVS 1500
            IKILENHVSDVSKVE+SVTNDLCS+ESK+LDEVEE SGRV+LNNIDVKWRMYAGSDWQVS
Sbjct: 1441 IKILENHVSDVSKVEYSVTNDLCSTESKKLDEVEEVSGRVILNNIDVKWRMYAGSDWQVS 1500

Query: 1501 RENGDPPMGMVKRDQHTCLELALSSMQ--------------------------------- 1560
             ENGDPPMGMVKRDQHTCLELAL+SMQ                                 
Sbjct: 1501 SENGDPPMGMVKRDQHTCLELALTSMQVQYDIFPVGGMCISRLSLSIQDFHLYDSSVDAP 1560

Query: 1561 ---VLGYYNSKNHPRKSSSKAFKLDLEAIRPDP-------------LSLL---------- 1620
               VLGYYNSKNHPRKSSSKAFKLDLEAIRPDP             L +L          
Sbjct: 1561 WKLVLGYYNSKNHPRKSSSKAFKLDLEAIRPDPSIPLEEYRLCIGILPMLLHLHQCQLDF 1620

Query: 1621 -------------RNTGQPPDSDASKTISSTKSQDGLTLAEEALLPYFQKFDIQPIVVRV 1680
                         R++GQP D D SKTIS+TKS DGLTLAEEALLPYFQKFDIQPIVVRV
Sbjct: 1621 LVNFFGERSSSRNRSSGQPLDLDGSKTISTTKSHDGLTLAEEALLPYFQKFDIQPIVVRV 1680

Query: 1681 DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS 1740
            DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS
Sbjct: 1681 DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS 1740

Query: 1741 HNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQRGTIAFLRSISLEA 1796
            HNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQRGTIAFLRSISLEA
Sbjct: 1741 HNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQRGTIAFLRSISLEA 1800

BLAST of PI0000519 vs. NCBI nr
Match: XP_008456130.1 (PREDICTED: autophagy-related protein 2 isoform X1 [Cucumis melo])

HSP 1 Score: 3255.7 bits (8440), Expect = 0.0e+00
Identity = 1681/1947 (86.34%), Postives = 1725/1947 (88.60%), Query Frame = 0

Query: 1    MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL 60
            MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL
Sbjct: 1    MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL 60

Query: 61   NVDFLNEKVGTAATVIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHMDCCG 120
            NVDFLNEKVGTAA+V FKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHM+ CG
Sbjct: 61   NVDFLNEKVGTAASVTFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHMNGCG 120

Query: 121  AFSGGHNKNHHESRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA 180
            AFSGGH+KNHH SRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA
Sbjct: 121  AFSGGHSKNHHGSRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA 180

Query: 181  FDSFYDENKNRTEFDTTLVLRIADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA 240
            FDSFYDENKNRTEFDTTLVLR+ADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA
Sbjct: 181  FDSFYDENKNRTEFDTTLVLRVADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA 240

Query: 241  MVEFLHMDDCDKAKTFSCMSAATSQMVLDHVPPNVATPFLTGGVGGFSGNLKLCIPLRDG 300
            MVEFLHMDDCDK KTFSCMSAATSQMVLDHVP NVATPFLTGGVGGFSGNLKLCIPLRDG
Sbjct: 241  MVEFLHMDDCDKEKTFSCMSAATSQMVLDHVPSNVATPFLTGGVGGFSGNLKLCIPLRDG 300

Query: 301  SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNGDRYINNKVNESDYFEQAS 360
            SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKN D  INNKVNESD FE+AS
Sbjct: 301  SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNSDGCINNKVNESDDFERAS 360

Query: 361  HRHSSALASAEMTPDETSPHCGGMLPGSHLISNWVPLSVKSREKEKVEEFDFGASVDQFF 420
            H HSS LASAEMTPDETSPHCGGMLPGSHLISNWVPLSVKS EKEKVEEFDFGASVDQFF
Sbjct: 361  HSHSSTLASAEMTPDETSPHCGGMLPGSHLISNWVPLSVKSGEKEKVEEFDFGASVDQFF 420

Query: 421  ECLDEIRSTQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV 480
            ECLDEIRS+QSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV
Sbjct: 421  ECLDEIRSSQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV 480

Query: 481  ISFHDDNKFHFTDAEKVQIKADSEVHFVAAKFSDIHLLMQ-------------------- 540
            ISF DDNK+HF D EKV+IKADSEVHFVAAKFSD+HLLMQ                    
Sbjct: 481  ISFRDDNKYHFADTEKVEIKADSEVHFVAAKFSDVHLLMQVSTQRTRFHGTIKHVEIADY 540

Query: 541  ---------TDFRNSNGDFQTILMKRLQVDVLGALPPFEFSAEDPDLVESNGSFNMDLPC 600
                     TDF NSNGDFQTILMKRLQVDVLGALPPF+FSA DPDL+ESN SFNM+LPC
Sbjct: 541  SNCNSYASKTDFCNSNGDFQTILMKRLQVDVLGALPPFDFSAGDPDLMESNSSFNMELPC 600

Query: 601  ENKDNVTKITLLETYGITSSQLNTTSSSNDNSTMTKSFSLNLPPFVFWVNYTLVNMLLDL 660
            ENKDNV KITLLETY ITSSQLN TS SNDNSTM+KSFSLNLPPFVFWVNYTLVNMLLDL
Sbjct: 601  ENKDNVAKITLLETYDITSSQLNMTSGSNDNSTMSKSFSLNLPPFVFWVNYTLVNMLLDL 660

Query: 661  LKDVANCMPGDNNHMCFKENYTSDHEDAKSSSNQVTALTLWAILPGIDI----------- 720
            L+DVANCMPGDNNHMCFKE YTSDHEDAKSSSNQVTAL+  ++   + I           
Sbjct: 661  LEDVANCMPGDNNHMCFKEKYTSDHEDAKSSSNQVTALSFSSMQGNVIISNARVIFCFPL 720

Query: 721  ---------------IALDFYSPPITKEETTHRGNLALQKSYLSQKNALHFRFGSLGVFL 780
                           IALDFYSPPITKEETTHRGNLALQKSYLSQKNALHF FGS+GVFL
Sbjct: 721  ESDKDFMGYSTWDRFIALDFYSPPITKEETTHRGNLALQKSYLSQKNALHFGFGSVGVFL 780

Query: 781  VTFEEDIKQSSTCNLQRKKFSAHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLAS 840
            VT EEDIKQSSTCNLQRKKFSAHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSL  
Sbjct: 781  VTSEEDIKQSSTCNLQRKKFSAHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLVC 840

Query: 841  LEESKSRCKFIGKDYEFASVANMKDMED------------STSVLHVSFSLVRINVGTVQ 900
            LEESKS CKFIGKDYEFASVANMKDME+            STSVLHVSF LVRINVGT Q
Sbjct: 841  LEESKSSCKFIGKDYEFASVANMKDMEESNLQTRQEMILSSTSVLHVSFPLVRINVGTAQ 900

Query: 901  YEAFHCLLDQLVKGLSSETCDVVDVTKGVACQTSILVDCNSLEIVIRPDSNESTKCSLQR 960
            Y+AFHCLLDQL+KGLS ETCDVVDVTKGVACQTSI+VDCNSLEIVIRPD NESTKCSLQR
Sbjct: 901  YKAFHCLLDQLIKGLSRETCDVVDVTKGVACQTSIVVDCNSLEIVIRPDLNESTKCSLQR 960

Query: 961  ELPGSWYHLRLEIQNFELISVTDLGGIKGGNFFWLAHGEGKLLGFISEDPDQEFLLISCS 1020
            ELPGSWYHLRLEIQNFELISV+DLGGIKG NFFWLAHGEGKLLGFISEDPDQEFLLISCS
Sbjct: 961  ELPGSWYHLRLEIQNFELISVSDLGGIKGANFFWLAHGEGKLLGFISEDPDQEFLLISCS 1020

Query: 1021 NSNMKRGDGEGSNALSSRLAGCDIVHSWDPESLQGFSSVTIRCATIFAIGGRLDWLDVIY 1080
            NSNMKRGDGEGSNALSSRLAGCDIVH WDPESLQGFSSVTIRCATI AIGGRLDWLDVIY
Sbjct: 1021 NSNMKRGDGEGSNALSSRLAGCDIVHLWDPESLQGFSSVTIRCATILAIGGRLDWLDVIY 1080

Query: 1081 SFFFLSSPPVEPEDDKIMTRENPKKSSGSYFFLNFVDVGLSYHPYLKNLLVQSGLSQTES 1140
            SFFFLSSPPVEP  DKIMTRENPK SSGS+FFLNF+DVGL+YHPYLKNLL+ SGLSQTES
Sbjct: 1081 SFFFLSSPPVEP-GDKIMTRENPKNSSGSHFFLNFIDVGLNYHPYLKNLLINSGLSQTES 1140

Query: 1141 SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHVFD 1200
            SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEH+ D
Sbjct: 1141 SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHLVD 1200

Query: 1201 AYSVEDLRKVGYVKVARETCIEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA 1260
            AYSVEDLRKVGYVKVARET IEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA
Sbjct: 1201 AYSVEDLRKVGYVKVARETFIEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA 1260

Query: 1261 AQLQQLFAPDLEESIVHLQTRWNNVQQGQERKEIDAGSSSPPCHNLSVKQSEVGLMDEIC 1320
            AQLQQLFAPDLEESIVHLQTRWNNVQQGQERKE+DA SSSPPCHNLSV QSEVGLMDEIC
Sbjct: 1261 AQLQQLFAPDLEESIVHLQTRWNNVQQGQERKEVDAESSSPPCHNLSVNQSEVGLMDEIC 1320

Query: 1321 EDAFLLNKNDSK-------------NVIIASQNVVCSSNSEVRETSSPAHSFMGSDPDGQ 1380
            EDAFLLNKN S+             N ++ ++  VCSSNSEV ETSSPA+SFMGSDPDGQ
Sbjct: 1321 EDAFLLNKNHSRECDYSKSKCSFSPNEVLHAE--VCSSNSEVCETSSPANSFMGSDPDGQ 1380

Query: 1381 TSFIQYRQFPEIIEGYCLSNLRSLPDLTIGRELHPEICNGRNSGSIDTGGRRSGWYGDLP 1440
            TSFIQYRQFPEIIEGYCLSNLRSLPDLT GRELHP+ICN RNSGSIDTGGRRSGWYGDLP
Sbjct: 1381 TSFIQYRQFPEIIEGYCLSNLRSLPDLTTGRELHPDICNARNSGSIDTGGRRSGWYGDLP 1440

Query: 1441 IKILENHVSDVSKVEHSVTNDLCSSESKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVS 1500
            IKILENHVSDVSKVEH VTNDLCS+ESKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVS
Sbjct: 1441 IKILENHVSDVSKVEHLVTNDLCSTESKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVS 1500

Query: 1501 RENGDPPMGMVKRDQHTCLELALSSMQ--------------------------------- 1560
            RENGDPPMGMVKRDQHTCLEL LSSMQ                                 
Sbjct: 1501 RENGDPPMGMVKRDQHTCLELVLSSMQVQYDIFPVGGMCISRLSLSVQDFHLYDSSVDAP 1560

Query: 1561 ---VLGYYNSKNHPRKSSSKAFKLDLEAIRPDPL-------------------------- 1620
               VLGYYNSKNHPRKSSSKAFKLDLEAIRPDPL                          
Sbjct: 1561 WKLVLGYYNSKNHPRKSSSKAFKLDLEAIRPDPLIPLEEYRLCIGILPILLHLHQCQLDF 1620

Query: 1621 ----------SLLRNTGQPPDSDASKTISSTKSQDGLTLAEEALLPYFQKFDIQPIVVRV 1680
                      S  R++GQP DSD SKTIS+TKS DGLTLAEEALLPYFQKFD+ P+VVRV
Sbjct: 1621 FVNFFGERSSSRNRSSGQPLDSDGSKTISTTKSHDGLTLAEEALLPYFQKFDMLPVVVRV 1680

Query: 1681 DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS 1740
            DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS
Sbjct: 1681 DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS 1740

Query: 1741 HNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQRGTIAFLRSISLEA 1796
            HNQIRKILEGLPAVRSLVAVGSGASKLV+SPVESYKKDRRILKGMQRGTIAFLRSISLEA
Sbjct: 1741 HNQIRKILEGLPAVRSLVAVGSGASKLVTSPVESYKKDRRILKGMQRGTIAFLRSISLEA 1800

BLAST of PI0000519 vs. NCBI nr
Match: KAA0037107.1 (autophagy-related protein 2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 3234.5 bits (8385), Expect = 0.0e+00
Identity = 1674/1947 (85.98%), Postives = 1718/1947 (88.24%), Query Frame = 0

Query: 1    MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL 60
            MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL
Sbjct: 1    MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL 60

Query: 61   NVDFLNEKVGTAATVIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHMDCCG 120
            N       VGTAA+V FKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHM+ CG
Sbjct: 61   N-------VGTAASVTFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHMNGCG 120

Query: 121  AFSGGHNKNHHESRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA 180
            AFSGGH+KNHH SRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA
Sbjct: 121  AFSGGHSKNHHGSRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVA 180

Query: 181  FDSFYDENKNRTEFDTTLVLRIADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA 240
            FDSFYDENKNRTEFDTTLVLR+ADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA
Sbjct: 181  FDSFYDENKNRTEFDTTLVLRVADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGA 240

Query: 241  MVEFLHMDDCDKAKTFSCMSAATSQMVLDHVPPNVATPFLTGGVGGFSGNLKLCIPLRDG 300
            MVEFLHMDDCDK KTFSCMSAATSQMVLDHVP NVATPFLTGGVGGFSGNLKLCIPLRDG
Sbjct: 241  MVEFLHMDDCDKEKTFSCMSAATSQMVLDHVPSNVATPFLTGGVGGFSGNLKLCIPLRDG 300

Query: 301  SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNGDRYINNKVNESDYFEQAS 360
            SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKN D  INNKVNESD FE+AS
Sbjct: 301  SLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNSDGCINNKVNESDDFERAS 360

Query: 361  HRHSSALASAEMTPDETSPHCGGMLPGSHLISNWVPLSVKSREKEKVEEFDFGASVDQFF 420
            H HSS LASAEMTPDETSPHCGGMLPGSHLISNWVPLSVKS EKEKVEEFDFGASVDQFF
Sbjct: 361  HSHSSTLASAEMTPDETSPHCGGMLPGSHLISNWVPLSVKSGEKEKVEEFDFGASVDQFF 420

Query: 421  ECLDEIRSTQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV 480
            ECLDEIRS+QSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV
Sbjct: 421  ECLDEIRSSQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIV 480

Query: 481  ISFHDDNKFHFTDAEKVQIKADSEVHFVAAKFSDIHLLMQ-------------------- 540
            ISF DDNK+HF D EKV+IKADSEVHFVAAKFSD+HLLMQ                    
Sbjct: 481  ISFRDDNKYHFADTEKVEIKADSEVHFVAAKFSDVHLLMQVSTQRTRFHGTIKHVEIADY 540

Query: 541  ---------TDFRNSNGDFQTILMKRLQVDVLGALPPFEFSAEDPDLVESNGSFNMDLPC 600
                     TDF NSNGDFQTILMKRLQVDVLGALPPF+FSA DPDL+ESN SFNM+LPC
Sbjct: 541  SNCNSYASKTDFCNSNGDFQTILMKRLQVDVLGALPPFDFSAGDPDLMESNSSFNMELPC 600

Query: 601  ENKDNVTKITLLETYGITSSQLNTTSSSNDNSTMTKSFSLNLPPFVFWVNYTLVNMLLDL 660
            ENKDNV KITLLETY ITSSQLN TS SNDNSTM+KSFSLNLPPFVFWVNYTLVNMLLDL
Sbjct: 601  ENKDNVAKITLLETYDITSSQLNMTSGSNDNSTMSKSFSLNLPPFVFWVNYTLVNMLLDL 660

Query: 661  LKDVANCMPGDNNHMCFKENYTSDHEDAKSSSNQVTALTLWAILPGIDI----------- 720
            L+DVANCMPGDNNHMCFKE YTSDHEDAKSSSNQVTAL+  ++   + I           
Sbjct: 661  LEDVANCMPGDNNHMCFKEKYTSDHEDAKSSSNQVTALSFSSMQGNVIISNARVIFCFPL 720

Query: 721  ---------------IALDFYSPPITKEETTHRGNLALQKSYLSQKNALHFRFGSLGVFL 780
                           IALDFYSPPITKEETTHRGNLALQKSYLSQKNALHF FGS+GVFL
Sbjct: 721  ESDKDFMGYSTWDRFIALDFYSPPITKEETTHRGNLALQKSYLSQKNALHFGFGSVGVFL 780

Query: 781  VTFEEDIKQSSTCNLQRKKFSAHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLAS 840
            VT EEDIKQSSTCNLQRKKFSAHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSL  
Sbjct: 781  VTSEEDIKQSSTCNLQRKKFSAHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLVC 840

Query: 841  LEESKSRCKFIGKDYEFASVANMKDMED------------STSVLHVSFSLVRINVGTVQ 900
            LEESKS CKFIGKDYEFASVANMKDME+            STSVLHVSF LVRINVGT Q
Sbjct: 841  LEESKSSCKFIGKDYEFASVANMKDMEESNLQTRQEMILSSTSVLHVSFPLVRINVGTAQ 900

Query: 901  YEAFHCLLDQLVKGLSSETCDVVDVTKGVACQTSILVDCNSLEIVIRPDSNESTKCSLQR 960
            Y+AFHCLLDQL+KGLS ETCDVVDVTKGVACQTSI+VDCNSLEIVIRPD NESTKCSLQR
Sbjct: 901  YKAFHCLLDQLIKGLSRETCDVVDVTKGVACQTSIVVDCNSLEIVIRPDLNESTKCSLQR 960

Query: 961  ELPGSWYHLRLEIQNFELISVTDLGGIKGGNFFWLAHGEGKLLGFISEDPDQEFLLISCS 1020
            ELPGSWYHLRLEIQNFELISV+DLGGIKG NFFWLAHGEGKLLGFISEDPDQEFLLISCS
Sbjct: 961  ELPGSWYHLRLEIQNFELISVSDLGGIKGANFFWLAHGEGKLLGFISEDPDQEFLLISCS 1020

Query: 1021 NSNMKRGDGEGSNALSSRLAGCDIVHSWDPESLQGFSSVTIRCATIFAIGGRLDWLDVIY 1080
            NSNMKRGDGEGSNALSSRLAGCDIVH WDPESLQGFSSVTIRCATI AIGGRLDWLDVIY
Sbjct: 1021 NSNMKRGDGEGSNALSSRLAGCDIVHLWDPESLQGFSSVTIRCATILAIGGRLDWLDVIY 1080

Query: 1081 SFFFLSSPPVEPEDDKIMTRENPKKSSGSYFFLNFVDVGLSYHPYLKNLLVQSGLSQTES 1140
            SFFFLSSPPVEP  DKIMTRENPK SSGS+FFLNF+DVGL+YHPYLKNLL+ SGLSQTES
Sbjct: 1081 SFFFLSSPPVEP-GDKIMTRENPKNSSGSHFFLNFIDVGLNYHPYLKNLLINSGLSQTES 1140

Query: 1141 SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHVFD 1200
            SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEH+ D
Sbjct: 1141 SSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHLVD 1200

Query: 1201 AYSVEDLRKVGYVKVARETCIEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA 1260
            AYSVEDLRKVGYVKVARET IEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA
Sbjct: 1201 AYSVEDLRKVGYVKVARETFIEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLA 1260

Query: 1261 AQLQQLFAPDLEESIVHLQTRWNNVQQGQERKEIDAGSSSPPCHNLSVKQSEVGLMDEIC 1320
            AQLQQLFAPDLEESIVHLQTRWNNVQQGQERKE+DA SSSPPCHNLSV QSEVGLMDEIC
Sbjct: 1261 AQLQQLFAPDLEESIVHLQTRWNNVQQGQERKEVDAESSSPPCHNLSVNQSEVGLMDEIC 1320

Query: 1321 EDAFLLNKNDSK-------------NVIIASQNVVCSSNSEVRETSSPAHSFMGSDPDGQ 1380
            EDAFLLNKN S+             N ++ ++  VCSSNSEV ETSSPA+SFMGSDPDGQ
Sbjct: 1321 EDAFLLNKNHSRECDYSKSKCSFSPNEVLHAE--VCSSNSEVCETSSPANSFMGSDPDGQ 1380

Query: 1381 TSFIQYRQFPEIIEGYCLSNLRSLPDLTIGRELHPEICNGRNSGSIDTGGRRSGWYGDLP 1440
            TSFIQYRQFPEIIEGYCLSNLRSLPDLT GRELHP+ICN RNSGSIDTGGRRSGWYGDLP
Sbjct: 1381 TSFIQYRQFPEIIEGYCLSNLRSLPDLTTGRELHPDICNARNSGSIDTGGRRSGWYGDLP 1440

Query: 1441 IKILENHVSDVSKVEHSVTNDLCSSESKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVS 1500
            IKILENHVSDVSKVEH VTNDLCS+ESKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVS
Sbjct: 1441 IKILENHVSDVSKVEHLVTNDLCSTESKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVS 1500

Query: 1501 RENGDPPMGMVKRDQHTCLELALSSMQ--------------------------------- 1560
            RENGDPPMGMVKRDQHTCLEL LSSMQ                                 
Sbjct: 1501 RENGDPPMGMVKRDQHTCLELVLSSMQVQYDIFPVGGMCISRLSLSVQDFHLYDSSVDAP 1560

Query: 1561 ---VLGYYNSKNHPRKSSSKAFKLDLEAIRPDPL-------------------------- 1620
               VLGYYNSKNHPRKSSSKAFKLDLEAIRPDPL                          
Sbjct: 1561 WKLVLGYYNSKNHPRKSSSKAFKLDLEAIRPDPLIPLEEYRLCIGILPILLHLHQCQLDF 1620

Query: 1621 ----------SLLRNTGQPPDSDASKTISSTKSQDGLTLAEEALLPYFQKFDIQPIVVRV 1680
                      S  R++GQP DSD SKTIS+TKS DGLTLAEEALLPYFQKFD+ P+VVRV
Sbjct: 1621 FVNFFGERSSSRNRSSGQPLDSDGSKTISTTKSHDGLTLAEEALLPYFQKFDMLPVVVRV 1680

Query: 1681 DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS 1740
            DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS
Sbjct: 1681 DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS 1740

Query: 1741 HNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQRGTIAFLRSISLEA 1796
            HNQIRKILEGLPAVRSLVAVGSGASKLV+SPVESYKKDRRILKGMQRGTIAFLRSISLEA
Sbjct: 1741 HNQIRKILEGLPAVRSLVAVGSGASKLVTSPVESYKKDRRILKGMQRGTIAFLRSISLEA 1800

BLAST of PI0000519 vs. NCBI nr
Match: KGN57557.2 (hypothetical protein Csa_010694 [Cucumis sativus])

HSP 1 Score: 3148.6 bits (8162), Expect = 0.0e+00
Identity = 1624/1880 (86.38%), Postives = 1664/1880 (88.51%), Query Frame = 0

Query: 68   KVGTAATVIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHMDCCGAFSGGHN 127
            +VGTAA+VIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHM+CCGAFSG H+
Sbjct: 52   QVGTAASVIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHMNCCGAFSGSHS 111

Query: 128  KNHHESRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVAFDSFYDE 187
             NHHESRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVAFDSFYDE
Sbjct: 112  NNHHESRKSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKIINLIVAFDSFYDE 171

Query: 188  NKNRTEFDTTLVLRIADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGAMVEFLHM 247
            NKNRTEFDTTLVLRIADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGAMVEFLHM
Sbjct: 172  NKNRTEFDTTLVLRIADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQGAMVEFLHM 231

Query: 248  DDCDKAKTFSCMSAATSQMVLDHVPPNVATPFLTGGVGGFSGNLKLCIPLRDGSLDIYRV 307
            DDCDKAKTF CMSAATSQMVLDHVP NVATPFLTGGVGGFSGNLKLCIPLRDGSLDIYRV
Sbjct: 232  DDCDKAKTFPCMSAATSQMVLDHVPSNVATPFLTGGVGGFSGNLKLCIPLRDGSLDIYRV 291

Query: 308  DGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNGDRYINNKVNESDYFEQASHRHSSAL 367
            DGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKN D  INNKVNESDYFE+A H HSSAL
Sbjct: 292  DGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNSDGCINNKVNESDYFERAFHSHSSAL 351

Query: 368  ASAEMTPDETSPHCGGMLPGSHLISNWVPLSVKSREKEKVEEFDFGASVDQFFECLDEIR 427
            ASAE TPDETSPHCGGMLPGSHLISNWVPLSVKSREKEKVEEFDFGASVDQFFECLDEIR
Sbjct: 352  ASAETTPDETSPHCGGMLPGSHLISNWVPLSVKSREKEKVEEFDFGASVDQFFECLDEIR 411

Query: 428  STQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIVISFHDDN 487
            STQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIVISFHDDN
Sbjct: 412  STQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISIVISFHDDN 471

Query: 488  KFHFTDAEKVQIKADSEVHFVAAKFSDIHLLMQ--------------------------- 547
            K+HFTD EKVQIKADSEVHFVAAKFSD+HLLMQ                           
Sbjct: 472  KYHFTDTEKVQIKADSEVHFVAAKFSDVHLLMQVSTQRTRFHGTIKHVEIADYLNCNSYA 531

Query: 548  --TDFRNSNGDFQTILMKRLQVDVLGALPPFEFSAEDPDLVESNGSFNMDLPCENKDNVT 607
              TDF NSNGDFQTILMKRLQVDVLGALPPF+FSAEDPDLVESN SFNMDLPCENKDNV 
Sbjct: 532  SKTDFCNSNGDFQTILMKRLQVDVLGALPPFDFSAEDPDLVESNSSFNMDLPCENKDNVA 591

Query: 608  KITLLETYGITSSQLNTTSSSNDNSTMTKSFSLNLPPFVFWVNYTLVNMLLDLLKDVANC 667
            KITLLETYGITSSQLN TSSSNDNSTM+KSFSLNLPPFVFWVNYTLVNMLLDLLKDVANC
Sbjct: 592  KITLLETYGITSSQLNMTSSSNDNSTMSKSFSLNLPPFVFWVNYTLVNMLLDLLKDVANC 651

Query: 668  MPGDNNHMCFKENYTSDHEDAKSSSNQVTALTLWAILPGIDI------------------ 727
            MPGDNNHMCFKENYTSDHEDAKSS NQVTAL+  ++   + I                  
Sbjct: 652  MPGDNNHMCFKENYTSDHEDAKSSPNQVTALSFSSMQGNVIISNARVIFCFPLESDKDFM 711

Query: 728  --------IALDFYSPPITKEETTHRGNLALQKSYLSQKNALHFRFGSLGVFLVTFEEDI 787
                    IALDFY+ PITKEETTHRGNLA+QKSY  QKNALHFRFGS+GVFLVTFEEDI
Sbjct: 712  GYSSWDRFIALDFYASPITKEETTHRGNLAVQKSYQLQKNALHFRFGSVGVFLVTFEEDI 771

Query: 788  KQSSTCNLQRKKFSAHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLASLEESKSR 847
            KQSSTCNLQ KKFS HNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLA LEESKS 
Sbjct: 772  KQSSTCNLQGKKFSVHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLACLEESKSS 831

Query: 848  CKFIGKDYEFASVANMKDMED------------STSVLHVSFSLVRINVGTVQYEAFHCL 907
            CKFIGKDYEFASVANMKDME+            STSVLHVSF LVRINVGTVQY+AFHCL
Sbjct: 832  CKFIGKDYEFASVANMKDMEESNLQTRQEMILSSTSVLHVSFPLVRINVGTVQYKAFHCL 891

Query: 908  LDQLVKGLSSETCDVVDVTKGVACQTSILVDCNSLEIVIRPDSNESTKCSLQRELPGSWY 967
            LDQL+KGLS ETCDVVDVTKGVACQTSI+VDCNSLEIVIRPD NESTKCSLQRELPGSWY
Sbjct: 892  LDQLIKGLSRETCDVVDVTKGVACQTSIVVDCNSLEIVIRPDLNESTKCSLQRELPGSWY 951

Query: 968  HLRLEIQNFELISVTDLGGIKGGNFFWLAHGEGKLLGFISEDPDQEFLLISCSNSNMKRG 1027
            HLRLEIQNFELISV+DLGGIKG NFFWLAHGEGKLLGFISEDPDQEFLLISCSNSNMKRG
Sbjct: 952  HLRLEIQNFELISVSDLGGIKGANFFWLAHGEGKLLGFISEDPDQEFLLISCSNSNMKRG 1011

Query: 1028 DGEGSNALSSRLAGCDIVHSWDPESLQGFSSVTIRCATIFAIGGRLDWLDVIYSFFFLSS 1087
            DGEGSNALSSRLAGCDIVH WDPESLQGFSSVTIRCATI AIGGRLDWLDVIYSFFFLSS
Sbjct: 1012 DGEGSNALSSRLAGCDIVHLWDPESLQGFSSVTIRCATILAIGGRLDWLDVIYSFFFLSS 1071

Query: 1088 PPVEPEDDKIMTRENPKKSSGSYFFLNFVDVGLSYHPYLKNLLVQSGLSQTESSSSTFKQ 1147
            PPVEPE DKIMTRENPK SSGS FFLNFVDVGL+YHPYLKNLL++SGLSQ+ESSSSTFKQ
Sbjct: 1072 PPVEPEGDKIMTRENPKNSSGSCFFLNFVDVGLNYHPYLKNLLIKSGLSQSESSSSTFKQ 1131

Query: 1148 ELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHVFDAYSVEDL 1207
            ELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHVFDAYSVEDL
Sbjct: 1132 ELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHVFDAYSVEDL 1191

Query: 1208 RKVGYVKVARETCIEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLAAQLQQLF 1267
            RKVGYVKVARET IEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLAAQLQQLF
Sbjct: 1192 RKVGYVKVARETFIEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLAAQLQQLF 1251

Query: 1268 APDLEESIVHLQTRWNNVQQGQERKEIDAGSSSPPCHNLSVKQSEVGLMDEICEDAFLLN 1327
            APDLEESIVHLQTRWNN QQGQERKEIDA SSSPPCHNLSV QSEVGLMDEICEDAFLLN
Sbjct: 1252 APDLEESIVHLQTRWNNAQQGQERKEIDAESSSPPCHNLSVNQSEVGLMDEICEDAFLLN 1311

Query: 1328 KNDSK-------------NVIIASQNVVCSSNSEVRETSSPAHSFMGSDPDGQTSFIQYR 1387
            KN S+             N ++ ++  VCSSNSEV ETSSPAHSFMGSDPDGQTSFIQYR
Sbjct: 1312 KNHSRECDYSKTKCSFSPNEVLHAE--VCSSNSEVCETSSPAHSFMGSDPDGQTSFIQYR 1371

Query: 1388 QFPEIIEGYCLSNLRSLPDLTIGRELHPEICNGRNSGSIDTGGRRSGWYGDLPIKILENH 1447
            QFPEIIEGYCLSNL SLPDLTIGRELHP+ICNGRNSGSIDTGGRRSGWYGDLPIKILENH
Sbjct: 1372 QFPEIIEGYCLSNLCSLPDLTIGRELHPDICNGRNSGSIDTGGRRSGWYGDLPIKILENH 1431

Query: 1448 VSDVSKVEHSVTNDLCSSESKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVSRENGDPP 1507
            VSDVSKVE+SVTNDLCS+ESK+LDEVEE SGRV+LNNIDVKWRMYAGSDWQVS ENGDPP
Sbjct: 1432 VSDVSKVEYSVTNDLCSTESKKLDEVEEVSGRVILNNIDVKWRMYAGSDWQVSSENGDPP 1491

Query: 1508 MGMVKRDQHTCLELALSSMQ------------------------------------VLGY 1567
            MGMVKRDQHTCLELAL+SMQ                                    VLGY
Sbjct: 1492 MGMVKRDQHTCLELALTSMQVQYDIFPVGGMCISRLSLSIQDFHLYDSSVDAPWKLVLGY 1551

Query: 1568 YNSKNHPRKSSSKAFKLDLEAIRPDP-------------LSLL----------------- 1627
            YNSKNHPRKSSSKAFKLDLEAIRPDP             L +L                 
Sbjct: 1552 YNSKNHPRKSSSKAFKLDLEAIRPDPSIPLEEYRLCIGILPMLLHLHQCQLDFLVNFFGE 1611

Query: 1628 ------RNTGQPPDSDASKTISSTKSQDGLTLAEEALLPYFQKFDIQPIVVRVDYSPSRV 1687
                  R++GQP D D SKTIS+TKS DGLTLAEEALLPYFQKFDIQPIVVRVDYSPSRV
Sbjct: 1612 RSSSRNRSSGQPLDLDGSKTISTTKSHDGLTLAEEALLPYFQKFDIQPIVVRVDYSPSRV 1671

Query: 1688 DLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDISHNQIRKI 1747
            DLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDISHNQIRKI
Sbjct: 1672 DLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDISHNQIRKI 1731

Query: 1748 LEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQRGTIAFLRSISLEAVGLGVHL 1796
            LEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQRGTIAFLRSISLEAVGLGVHL
Sbjct: 1732 LEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQRGTIAFLRSISLEAVGLGVHL 1791

BLAST of PI0000519 vs. NCBI nr
Match: XP_038897510.1 (autophagy-related protein 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 3129.0 bits (8111), Expect = 0.0e+00
Identity = 1633/1947 (83.87%), Postives = 1695/1947 (87.06%), Query Frame = 0

Query: 1    MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL 60
            MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL
Sbjct: 1    MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL 60

Query: 61   NVDFLNEKVGTAATVIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNVHMDCCG 120
            NVDFLNEKVGTAA VIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKN HMDCCG
Sbjct: 61   NVDFLNEKVGTAALVIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLKNDHMDCCG 120

Query: 121  AFSGGHNKNHHESRKSEHDVVKNAAKSTY-GDIHEGVKTVAKMVKGLLASFHLKIINLIV 180
             FSGGH KNHHESRKSEHDVVKNAAKSTY GDIHEGVKTVAKMVKGLLASFHLKIINLIV
Sbjct: 121  TFSGGH-KNHHESRKSEHDVVKNAAKSTYGGDIHEGVKTVAKMVKGLLASFHLKIINLIV 180

Query: 181  AFDSFYDENKNRTEFDTTLVLRIADVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQG 240
            AFDSFYDENK RT FDTTLVLRI+DVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQG
Sbjct: 181  AFDSFYDENKTRTGFDTTLVLRISDVECGTCVTEDGKLGMDAVESFLGISQLNNFVKFQG 240

Query: 241  AMVEFLHMDDCDKAKTFSCMSAATSQMVLDHVPPNVATPFLTGGVGGFSGNLKLCIPLRD 300
            AMVEFLHMDDCDK KTFSC +AA+SQMVLDHVP NVATPFLTGGVGGFSGNLKLCIPLRD
Sbjct: 241  AMVEFLHMDDCDKVKTFSCTTAASSQMVLDHVPSNVATPFLTGGVGGFSGNLKLCIPLRD 300

Query: 301  GSLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNGDRYINNKVNESDYFEQA 360
            GSLDI+RVDGDLSFDPVQ+KLQPRT+KC L LSEAYWNSDKN D  I+NKVNES YFEQA
Sbjct: 301  GSLDIHRVDGDLSFDPVQVKLQPRTVKCFLILSEAYWNSDKNSDGCIHNKVNESGYFEQA 360

Query: 361  SHRHSSALASAEMTPDETSPHCGGMLPGSHLISNWVPLSVKSREKEKVEEFDFGASVDQF 420
            S   SS LASAEMTPDETSPH GG+LPGSHLISNWVPLSVK REKEKVEEFDFGASVDQF
Sbjct: 361  S---SSGLASAEMTPDETSPHGGGILPGSHLISNWVPLSVKCREKEKVEEFDFGASVDQF 420

Query: 421  FECLDEIRSTQSALGSSGMWNSVFSAITAASSLASGSLHVPSELQPVETNLRATISGISI 480
            FECLDEIRSTQSALGSSGMWNSVFSAITAASSLA+GSLHVPSELQPVETNLRA ISGIS+
Sbjct: 421  FECLDEIRSTQSALGSSGMWNSVFSAITAASSLATGSLHVPSELQPVETNLRAAISGISV 480

Query: 481  VISFHDDNKFHFTDAEKVQIKADSEVHFVAAKFSDIHLLMQ------------------- 540
            VISFHDDNK+HFTD +K QIKADSEVHFVAAKFSDIHLLMQ                   
Sbjct: 481  VISFHDDNKYHFTDTKKAQIKADSEVHFVAAKFSDIHLLMQVSTQRTRFHGTIKHIEVAD 540

Query: 541  ----------TDFRNSNGDFQTILMKRLQVDVLGALPPFEFSAEDPDLVESNGSFNMDLP 600
                      TDF NSNGDFQT+LMKRLQVDVL ALPPF+FSAEDPDLVESN SFNMD P
Sbjct: 541  YLNCRSYASKTDFHNSNGDFQTVLMKRLQVDVLDALPPFDFSAEDPDLVESNSSFNMDFP 600

Query: 601  CENKDNVTKITLLETYGITSSQLNTTSSSND-NSTMTKSFSLNLPPFVFWVNYTLVNMLL 660
            CENKDNVTKITLL+TYGITSSQLNTTSSS+D NSTM+KSFSLNLPPFVFWVNYTLV MLL
Sbjct: 601  CENKDNVTKITLLKTYGITSSQLNTTSSSSDENSTMSKSFSLNLPPFVFWVNYTLVKMLL 660

Query: 661  DLLKDVANCMPGDNNHMCFKENYTSDHEDAKSSSNQVTALTLWAILPGIDI--------- 720
            DLLK V NCMPGDNNH CFKENYTSDHEDAKSSSNQVTAL+  ++   I I         
Sbjct: 661  DLLKGVENCMPGDNNHKCFKENYTSDHEDAKSSSNQVTALSSSSMQGNIIISNARVIFCF 720

Query: 721  ----------------IALDFYSPPITKEETTHRGNLALQKSYLSQKNALHFRFGSLGVF 780
                            IALDFYSPPITKEETTHRGNLALQKSYLS+KNALHFRFGSLGVF
Sbjct: 721  PLESDKDLGYTSWDRFIALDFYSPPITKEETTHRGNLALQKSYLSEKNALHFRFGSLGVF 780

Query: 781  LVTFEEDIKQSSTCNLQRKKFSAHNILSASNRTNGSPLTLFWQEGHVTGPWIAKKAKSLA 840
            LVTFEEDI QSSTCN+QRKKF AHNILSASNRTNGS LTLFWQE +VTGPWIAKKAKSLA
Sbjct: 781  LVTFEEDINQSSTCNVQRKKFLAHNILSASNRTNGSSLTLFWQEVNVTGPWIAKKAKSLA 840

Query: 841  SLEESKSRCKFIGKDYEFASVANMKDMED------------STSVLHVSFSLVRINVGTV 900
            +LEESKSRCKFIGKDYEFASVANMKD+E+            ST VLHV FSLVRIN GTV
Sbjct: 841  NLEESKSRCKFIGKDYEFASVANMKDVEESNLQTRQEMILSSTLVLHVFFSLVRINFGTV 900

Query: 901  QYEAFHCLLDQLVKGLSSETCDVVDVTKG-VACQTSILVDCNSLEIVIRPDSNESTKCSL 960
            QY+AFH LLDQL+KGLSSETCD VDVTKG VACQTSI+VDCN LEI IRPDSNESTKC L
Sbjct: 901  QYKAFHFLLDQLIKGLSSETCDAVDVTKGLVACQTSIVVDCNCLEIAIRPDSNESTKCYL 960

Query: 961  QRELPGSWYHLRLEIQNFELISVTDLGGIKGGNFFWLAHGEGKLLGFISEDPDQEFLLIS 1020
            QRELPGSWYHLRL+IQN ELISV+DLGGIKG NFFWLAHGEGKLLGFISEDPDQEFLLIS
Sbjct: 961  QRELPGSWYHLRLDIQNLELISVSDLGGIKGANFFWLAHGEGKLLGFISEDPDQEFLLIS 1020

Query: 1021 CSNSNMKRGDGEGSNALSSRLAGCDIVHSWDPESLQGFSSVTIRCATIFAIGGRLDWLDV 1080
            CSNSNMKRGDGEGSNALSSRLAG DI+H WDP+SLQGFSS+TIRCATI AIGGRLDWLDV
Sbjct: 1021 CSNSNMKRGDGEGSNALSSRLAGSDIMHLWDPDSLQGFSSITIRCATILAIGGRLDWLDV 1080

Query: 1081 IYSFFFLSSPPVEPEDDKIMTRENPKKSSGSYFFLNFVDVGLSYHPYLKNLLVQSGLSQT 1140
            IYSFFFLSSPPVEPE ++IM RE PKKSSGS FFLNFVDVGL+YHPYLKNLLV+SGLSQT
Sbjct: 1081 IYSFFFLSSPPVEPEGNEIMPREKPKKSSGSCFFLNFVDVGLNYHPYLKNLLVKSGLSQT 1140

Query: 1141 ESSSSTFKQELDDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHV 1200
            ESSSSTFKQEL+DDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHV
Sbjct: 1141 ESSSSTFKQELEDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHV 1200

Query: 1201 FDAYSVEDLRKVGYVKVARETCIEAILRTNCNNGLKWELECGKAHISVETCHDTASGLAR 1260
            FDAY+V DLRKVGYVKVARET IEAILRTNCNNGLKWELEC KAHISVETCHDTASGLAR
Sbjct: 1201 FDAYNVGDLRKVGYVKVARETFIEAILRTNCNNGLKWELECYKAHISVETCHDTASGLAR 1260

Query: 1261 LAAQLQQLFAPDLEESIVHLQTRWNNVQQGQERKEIDAGSSSPPCHNLSVKQSEVGLMDE 1320
            LAAQLQQLFAPDLEESIVHLQTRWNNVQQGQERKEID  SSSPPCHNLSVKQSEVGLMDE
Sbjct: 1261 LAAQLQQLFAPDLEESIVHLQTRWNNVQQGQERKEIDTESSSPPCHNLSVKQSEVGLMDE 1320

Query: 1321 ICEDAFLLNKNDSKNV--------IIASQNV---VCSSNSEVRETSSPAHSFMGSDPDGQ 1380
            ICEDAFLLNKN S+          ++ ++ +   VCSSNS+VRETSSPAHSFMGSDPDGQ
Sbjct: 1321 ICEDAFLLNKNHSRECDYSKSKCSVLPNEGLHAEVCSSNSDVRETSSPAHSFMGSDPDGQ 1380

Query: 1381 TSFIQYRQFPEIIEGYCLSNLRSLPDLTIGRELHPEICNGRNSGSIDTGGRRSGWYGDLP 1440
            TSFIQYRQFPEIIEGYCLS LR L DL IGR+L  +I N RNSGSIDTG RRSGWYGDLP
Sbjct: 1381 TSFIQYRQFPEIIEGYCLSELRPLSDLAIGRQLPSDISNSRNSGSIDTGERRSGWYGDLP 1440

Query: 1441 IKILENHVSDVSKVEHSVTNDLCSSESKRLDEVEEASGRVLLNNIDVKWRMYAGSDWQVS 1500
            +KILENH+SDVSKVEH VT+DLCS+ES  LDEVEE SGRVLLNNIDVKWRMYAGSDWQVS
Sbjct: 1441 MKILENHISDVSKVEHLVTDDLCSNESISLDEVEEPSGRVLLNNIDVKWRMYAGSDWQVS 1500

Query: 1501 RENGDPPMGMVKRDQHTCLELALSSMQ--------------------------------- 1560
            + N D PMG VKRDQHTCLELALSSMQ                                 
Sbjct: 1501 KVNSDLPMGTVKRDQHTCLELALSSMQVQYDIFPVGGMCVSRLSLSVQDFHLYDSSVDAP 1560

Query: 1561 ---VLGYYNSKNHPRKSSSKAFKLDLEAIRPDPLSLL----------------------- 1620
               VLGYYNSKNHPRKSSSKAFKLDLEAIRPDPL  L                       
Sbjct: 1561 WKLVLGYYNSKNHPRKSSSKAFKLDLEAIRPDPLIPLEEYRLCIGFLPMILHLHQCQLDF 1620

Query: 1621 -------------RNTGQPPDSDASKTISSTKSQDGLTLAEEALLPYFQKFDIQPIVVRV 1680
                         R++G P DSD  KTI +T+S DGLTLAEEALLPYFQKFDIQPIVVRV
Sbjct: 1621 LVNFFGERSSSGNRSSG-PLDSDGLKTIPTTRSHDGLTLAEEALLPYFQKFDIQPIVVRV 1680

Query: 1681 DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS 1740
            DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS
Sbjct: 1681 DYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCETVVGEWLEDIS 1740

Query: 1741 HNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQRGTIAFLRSISLEA 1796
            HNQIRKILEGLPAVRSLVAVGSGASKL+SSPVESYKKDRRILKGMQRGTIAFLRSISLEA
Sbjct: 1741 HNQIRKILEGLPAVRSLVAVGSGASKLLSSPVESYKKDRRILKGMQRGTIAFLRSISLEA 1800

BLAST of PI0000519 vs. TAIR 10
Match: AT3G19190.1 (autophagy 2 )

HSP 1 Score: 1395.9 bits (3612), Expect = 0.0e+00
Identity = 847/1900 (44.58%), Postives = 1133/1900 (59.63%), Query Frame = 0

Query: 1    MFPWNIAKTAEAMFSKFAVKRLCKFLLKKKLGQFLLGEIDIDQLDVQLADGTIQLSDLAL 60
            +FPWNIAK+AE  FS++AVKR+ KFLLKKKLG+ +LG+ID+DQLD+QL DGTIQLSDLA+
Sbjct: 2    VFPWNIAKSAEEAFSRWAVKRVVKFLLKKKLGKLILGDIDLDQLDIQLRDGTIQLSDLAI 61

Query: 61   NVDFLNEKVGTAATVIFKEGSIGSLLIRMPWTSRGCEVEINGLELVLSPCLK-NVHMDCC 120
            NVD+LN+K    A ++ KEGSIGSLL++MPW + GC+VE++ LELVL+P L+ N      
Sbjct: 62   NVDYLNDKFD--APLVIKEGSIGSLLVKMPWKTNGCQVEVDELELVLAPRLESNKSSSNE 121

Query: 121  GAFSGGHNKNHHESR----KSEHDVVKNAAKSTYGDIHEGVKTVAKMVKGLLASFHLKII 180
             + S    ++ H  R    K E++++ NAAKS   D+HEGVKTVAK+VK  L SFH+KI 
Sbjct: 122  ASTSASTREDLHNIRLEIGKHENEMLMNAAKSASIDVHEGVKTVAKIVKWFLTSFHVKIK 181

Query: 181  NLIVAFDSFYDENKNRTEFDTTLVLRIADVECGTCVTEDGKLGMDAVESFLGISQLNNFV 240
            NLI+AFD  + + ++      TLVLR+ ++ECG    E       + ++FLGI++L N V
Sbjct: 182  NLIIAFDPDFGKKQSEAGPRPTLVLRMTEIECGIS-EEQVSANEVSPDNFLGINRLANCV 241

Query: 241  KFQGAMVEFLHMDDCDKAKTFSCMSAATSQMVLDHVPPNVATPFLTGGVGGFSGNLKLCI 300
            KFQGA+VE L+MDD D                 D    N  T  +TG  GGFSG+L   I
Sbjct: 242  KFQGAVVELLNMDDDD-----------DGDKTCDKKTSNDVTLIMTGVGGGFSGSLNFSI 301

Query: 301  PLRDGSLDIYRVDGDLSFDPVQLKLQPRTIKCLLTLSEAYWNSDKNGDRYINNKVNESDY 360
            P ++GSLDI +VD D+S DPV+++ QP TI+  L L    W +  +        V+ SD+
Sbjct: 302  PWKNGSLDIRKVDADISIDPVEVRFQPSTIRWFLQL----WKTFTSFGSDCFPSVSHSDF 361

Query: 361  FEQASHRHSSALASAEMTPDETSPHCGGM------LPGSHLISNWVPLSVKSREKEKVEE 420
               +    ++ +    +TP  T    GG        P    I +W P S   +E++   E
Sbjct: 362  LTDSPTIPTNVM----VTPPATLSLSGGQELEHDTTPNLQFIPDWFPSSFSKKEED--GE 421

Query: 421  FDFGASVDQFFECLDEIRSTQSALGSSGMWN---SVFSAITAASSLASGSLHVPSELQPV 480
             D GASVDQFFEC D +RS QSA GS GMWN   SVF+AI AASSLASGSL +PSE Q V
Sbjct: 422  VDIGASVDQFFECFDAMRSYQSASGSQGMWNWTSSVFTAINAASSLASGSLLLPSEQQHV 481

Query: 481  ETNLRATISGISIVISFHDD-------NKFHFTDAEKVQIKADSEVHFVAAKF-SDIHLL 540
            ET+ + + +G+S+V+ F D+        + H+  AE   I    +V     +   +++ +
Sbjct: 482  ETSCKVSFAGVSVVLFFQDEVNWKGVSTRIHYLGAELRDISVSFQVCLHDLRLEGEVNSM 541

Query: 541  MQTDF-------RNSNGDFQTILMKRLQVDVLGALPPFEFSAEDPDLVESNGSFNMDLPC 600
               D+         +N + QT L+K LQ  V  +LPPF  S    D    +   +     
Sbjct: 542  EIADYCQGGNVVDTANAESQTCLIKDLQAKVQTSLPPFASSDMHSDSERLSEIVSDGFLF 601

Query: 601  ENKDNVTKITLLETYGITSSQLNTT-SSSNDNSTMTKSFSLNLPPFVFWVNYTLVNMLLD 660
             NK    K  L+   G +  Q      SS  +   + SFSL+LPP  FW+N   V ML++
Sbjct: 602  RNKGFAVKTLLVIAAGGSGFQFTVNFQSSKASHRGSNSFSLSLPPTTFWLNLHSVEMLVN 661

Query: 661  LLKDVANCMPGDNNHMCFKENYTSDHEDAKSSSNQVTA-LTLWAILPGI----------D 720
            L  DV+  +P   +H   +   +S  E  + S +   A + LW     I           
Sbjct: 662  LFNDVSESIP-ITSHERNQVASSSKSESLRGSVSICNARVILWFPFESISERFCNSLGQQ 721

Query: 721  IIALDF-YSPPITKEETTHRGNLALQKSYLSQKNALHFRFGSLGVFLVTFEEDIKQSSTC 780
             I +D   SPP  KE    R     +  + S   ++ F  G   ++LVT   D+K S T 
Sbjct: 722  FIVVDLSSSPPSDKERAKERS--PGEMHFPSATRSICFSVGDASIYLVT--SDLKDSETN 781

Query: 781  NLQRK-KFSAHNILSASNRTNG--SPLTLFWQEGHVTGPWIAKKAKSLASLEESKSRCKF 840
            +  R+ +FSA+NIL  +N+T    S + +FWQ+     PW+ ++AK LA+ EES    K 
Sbjct: 782  SYHRQVEFSAYNILHTNNKTRHQLSTIGMFWQDRPTVSPWLVERAKMLATQEESIQTDKS 841

Query: 841  IGKDYEFASVANMKDMED------------STSVLHVSFSLVRINVGTVQYEAFHCLLDQ 900
             G+  EFA+VA  KD +D            S+  L+V    + I++ + QY     L+++
Sbjct: 842  GGRGLEFAAVATPKDQDDIYSRSRKEIILASSFCLYVHLLPLAIHLDSWQYSKLCNLIEE 901

Query: 901  LVKGLSSETCDVVDVT-KGVACQTSILVDCNSLEIVIRPDSNESTKCSLQRELPGSWYHL 960
                LS    +  + T + V CQTS++VDC+S++I++RP+     K  LQ ELPGSW   
Sbjct: 902  AKNWLSRMAANTAEQTEESVVCQTSLVVDCDSIDILVRPEPRMGIKKQLQTELPGSWIQF 961

Query: 961  RLEIQNFELISVTDLGGIKGGNFFWLAHGEGKLLGFISEDPDQEFLLISCSNSNMKRGDG 1020
             L +Q   L+SV +LG + G +FFWLAHGEG LLG ++  PDQE LL+SC+NS +KRG+G
Sbjct: 962  NLRVQKLNLMSVPNLGSVSGADFFWLAHGEGTLLGSVTGLPDQELLLLSCNNSAIKRGNG 1021

Query: 1021 EGSNALSSRLAGCDIVHSWDPESLQGFSSVTIRCATIFAIGGRLDWLDVIYSFFFLSSPP 1080
             GSNALSSR AG D +H  +P     + +V+ R  TI A+GGRLDW++V  SFF      
Sbjct: 1022 GGSNALSSRFAGLDFLHLQEPGICNDYLAVSARGCTISAVGGRLDWIEVATSFFSF---- 1081

Query: 1081 VEPEDDKIMTRENPKKSSGSYFFLNFVDVGLSYHPYLKNLLVQSGLSQTESSSSTFKQEL 1140
               ED+K     N   SSGS F LNFVDVGLSY P+ +N              +   ++ 
Sbjct: 1082 ---EDEKKTQEINSSSSSGSSFILNFVDVGLSYEPHHEN--------------TDHLRQA 1141

Query: 1141 DDDYVACLLAASSVTLSSSSVADVVEDNYRITVQDFGLLLCSVSDYEHVFDAYSVEDLRK 1200
             D +VACL+AASS +LS  S+ D + ++YRI +QD GLLL    D   +   YS E L +
Sbjct: 1142 SDPWVACLVAASSFSLSKKSLVDSIRNDYRIRIQDLGLLLSVDFDLSKLGGTYSSEHLHE 1201

Query: 1201 VGYVKVARETCIEAILRTNCNNGLKWELECGKAHISVETCHDTASGLARLAAQLQQLFAP 1260
             GYVKVA ++ IEAILRTN  NGL WELEC K+H+ +ETC DT SGL RLA QLQQL AP
Sbjct: 1202 SGYVKVANDSLIEAILRTNSENGLLWELECSKSHLVIETCSDTTSGLIRLATQLQQLLAP 1261

Query: 1261 DLEESIVHLQTRWNNVQQGQERKEIDAG---SSSPPCHNLSVKQSE-------VGLMDEI 1320
            DLEES VHLQTRW+++QQ   R ++D     SSS     +   + E       +GLMDEI
Sbjct: 1262 DLEESAVHLQTRWDSIQQANARNDLDISDRLSSSDSSGEMKYLRLESENETGVIGLMDEI 1321

Query: 1321 CEDAFLLNKNDSKNVIIASQNVVCSSN-------------------SEVRETSSPAHSFM 1380
             EDAF  + N +      S +V C +N                    ++    S   S  
Sbjct: 1322 NEDAFQFDVNPT----YQSDSVECQNNYMSPHGISHGQAYNWVPATEKLPSNQSICGSSS 1381

Query: 1381 GSDPDGQTSFIQYRQFPEIIEGYCLSNLRSLPDL-----TIGRELHPEICNGRNSGSIDT 1440
              + +    F++    PEI E YCLS  R   ++     + GREL PE          D 
Sbjct: 1382 RINSESSQVFLERESLPEIFENYCLSEFRPSSEVPQEGDSSGRELFPE---------TDL 1441

Query: 1441 GGRRSGWYGDLPIKILENHVSDVSKVEHS--VTNDLCSSESKRLDEVEEASGRVLLNNID 1500
                SGWY D  ++I+E+HVS+ ++ +H   + +  CSS  +       A+GR+LL NID
Sbjct: 1442 RRGNSGWYDDASLRIVEDHVSEATEEDHEEHILDGECSSFGQTSYSAVAANGRILLKNID 1501

Query: 1501 VKWRMYAGSDWQVSRENGDPPMGMVKRDQHTCLELALSSMQVLGYYNSKNHPRKSSSKAF 1560
            +KWR+Y+GSDW  SR+ G+       RD  +CLEL LS   VLGYYNSK+HPR SSS AF
Sbjct: 1502 LKWRIYSGSDWHDSRKKGENFKHTKGRDTTSCLELELSG--VLGYYNSKDHPRDSSSYAF 1561

Query: 1561 KLDLEAIRPDPLSLLR-------NTGQPPDSDASKTISSTK--SQDGLTLAEEALLPYFQ 1620
            KL+L+A+RPDP + L        N+ + P      +  ST   S  G  + EEALLPYFQ
Sbjct: 1562 KLELKAVRPDPETPLEENRFFGANSLEKPVVSMGDSGGSTMSVSVQGHNIIEEALLPYFQ 1621

Query: 1621 KFDIQPIVVRVDYSPSRVDLAALRGGKYVELVNLVPWKGVELHLKHVQAVGVYGWGSVCE 1680
            KFDI P+ VRVDYSP  VD+AAL GGKY ELVNLVPWKG+EL LKHV A G+YGWG+VCE
Sbjct: 1622 KFDIWPVNVRVDYSPHHVDIAALTGGKYAELVNLVPWKGIELQLKHVHAAGIYGWGNVCE 1681

Query: 1681 TVVGEWLEDISHNQIRKILEGLPAVRSLVAVGSGASKLVSSPVESYKKDRRILKGMQRGT 1740
            T++GEWLED+S NQI ++L+G+P VRSL A+ + A KLVSSPVESY+KDRR++KG+QRGT
Sbjct: 1682 TILGEWLEDVSQNQIHQLLKGIPTVRSLSALYAAALKLVSSPVESYRKDRRLVKGVQRGT 1741

Query: 1741 IAFLRSISLEAVGLGVHLAAGAHDILLQAEYILTSIPPSVKVRHKTRPNVRSNQPKDAQE 1796
            +AFLRSISLEAVGLGVHLAAGAHDILL+AEYI  S P   + + +T+ NVR NQP++A++
Sbjct: 1742 VAFLRSISLEAVGLGVHLAAGAHDILLRAEYIFASSPSLPQPQGRTKTNVRHNQPRNAKQ 1801