Moc11g30770 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc11g30770
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionUlp1-like peptidase
Locationchr11: 22462236 .. 22463390 (-)
RNA-Seq ExpressionMoc11g30770
SyntenyMoc11g30770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGAGGATCCGATGATTATGGATGAAGACCAAGGTACGATAACGGAGGGGGACAATGCATCGGATGCTTACCCGAACTCACGTGTGTATTGTGTTTTAGGTTGAACCTTACTTTGACCAGGACGAATATGACCTTCAGTAGGCCCCAACTGGGCGTGGGCTACGTAAGAGACATTACTCGTGGAAGCTGAAGGATATATACACACCAACCGGTCAGTGTGGGATCATCGTGGATAGCTACGACCCAGTATGTCCCATTACGCCGCAGTTGGATGATAAGTTCCAGAGATGGATGGATGACCCGAAGACGGACAAGAGATTGTTGTCCACTGCAACTGGTTTCCAAAAGAAGGAATGGTATCGCGATCTATTGGACCCTCGTGTTGAATTGAAGGACGAAGTAAGTACATGTTACTATATTCGTAGTACTATATTCGTCAATAGTTTTATTACATCAATGTGGATTGGTTAAATGTAATAGGTACTTGATGGTCTCATCCTGTTTACGGCGAAAAAGTTGGAGAAGTGTCTCCATCTATGTCACAAGAAGTTTGCGATAGGCGACGTACTTCTTTCAGTAATTACTTTTTACTTCTTTTGGTTCGAACTAATAAATGTTGCTTTGATCAATTGTTTAACTAATGCAATACTTTTTCAACTACTATACAGACTCTGCTGAATCGAACAGACAATCCATATGCGGCCATGAAGCCGGGTGTATTGTCCACTAGGATCAACTACCCCTGGAGCGAGGAGAATACCATCTTTCGATATGTTCACGGTAGGCAGTCAGACCACAACGTGCCCTAGAGTGATGTAGACATCGTGTATACCCCCATGAACGTAGGCAGGAACCACTCGGTGATGCTCGGCATCGACCTTGTACAGGGCGACATAACCGTATGGGATTCACTCCAAACAGTCACTCCACTAGATGAACTTGAGAAGGAGTTGAAGCTCATGTGTACGATCCTACCTACGTTGCTGCATCATGGCGGGATATTTTCAGTTCGACCCGACTTGCCAGTGGTGCCGTGGAGGGTGCGTCGGGTTCGCGTACCACAGCAGAGTAGCGCGACTGATTGCGAGATTTTCTGTGTCCGATTTTTCAAATACGATGCCACTAAGTCAAATATGGATACTTTGACCTAA

mRNA sequence

ATGGACGAGGATCCGATGATTATGGATGAAGACCAAGGTACGATAACGGAGGGGGACAATGCATCGGATGCTTACCCGAACTCACGTGTGTATTGTTGTGGGATCATCGTGGATAGCTACGACCCAGTATGTCCCATTACGCCGCAGTTGGATGATAAGTTCCAGAGATGGATGGATGACCCGAAGACGGACAAGAGATTGTTGTCCACTGCAACTGGTTTCCAAAAGAAGGAATGGTATCGCGATCTATTGGACCCTCGTGTTGAATTGAAGGACGAAGTACTTGATGGTCTCATCCTGTTTACGGCGAAAAAGTTGGAGAAGTGTCTCCATCTATGTCACAAGAAGTTTGCGATAGGCGACGTACTTCTTTCAACTCTGCTGAATCGAACAGACAATCCATATGCGGCCATGAAGCCGGGTGTATTGTCCACTAGGATCAACTACCCCTGGAGCGAGGAGAATACCATCTTTCGATATGTTCACGGCAGGAACCACTCGGTGATGCTCGGCATCGACCTTGTACAGGGCGACATAACCGTATGGGATTCACTCCAAACAGTCACTCCACTAGATGAACTTGAGAAGGAGTTGAAGCTCATGTGTACGATCCTACCTACGTTGCTGCATCATGGCGGGATATTTTCAGTTCGACCCGACTTGCCAGTGGTGCCGTGGAGGGTGCGTCGGGTTCGCGTACCACAGCAGAGTAGCGCGACTGATTGCGAGATTTTCTGTGTCCGATTTTTCAAATACGATGCCACTAAGTCAAATATGGATACTTTGACCTAA

Coding sequence (CDS)

ATGGACGAGGATCCGATGATTATGGATGAAGACCAAGGTACGATAACGGAGGGGGACAATGCATCGGATGCTTACCCGAACTCACGTGTGTATTGTTGTGGGATCATCGTGGATAGCTACGACCCAGTATGTCCCATTACGCCGCAGTTGGATGATAAGTTCCAGAGATGGATGGATGACCCGAAGACGGACAAGAGATTGTTGTCCACTGCAACTGGTTTCCAAAAGAAGGAATGGTATCGCGATCTATTGGACCCTCGTGTTGAATTGAAGGACGAAGTACTTGATGGTCTCATCCTGTTTACGGCGAAAAAGTTGGAGAAGTGTCTCCATCTATGTCACAAGAAGTTTGCGATAGGCGACGTACTTCTTTCAACTCTGCTGAATCGAACAGACAATCCATATGCGGCCATGAAGCCGGGTGTATTGTCCACTAGGATCAACTACCCCTGGAGCGAGGAGAATACCATCTTTCGATATGTTCACGGCAGGAACCACTCGGTGATGCTCGGCATCGACCTTGTACAGGGCGACATAACCGTATGGGATTCACTCCAAACAGTCACTCCACTAGATGAACTTGAGAAGGAGTTGAAGCTCATGTGTACGATCCTACCTACGTTGCTGCATCATGGCGGGATATTTTCAGTTCGACCCGACTTGCCAGTGGTGCCGTGGAGGGTGCGTCGGGTTCGCGTACCACAGCAGAGTAGCGCGACTGATTGCGAGATTTTCTGTGTCCGATTTTTCAAATACGATGCCACTAAGTCAAATATGGATACTTTGACCTAA

Protein sequence

MDEDPMIMDEDQGTITEGDNASDAYPNSRVYCCGIIVDSYDPVCPITPQLDDKFQRWMDDPKTDKRLLSTATGFQKKEWYRDLLDPRVELKDEVLDGLILFTAKKLEKCLHLCHKKFAIGDVLLSTLLNRTDNPYAAMKPGVLSTRINYPWSEENTIFRYVHGRNHSVMLGIDLVQGDITVWDSLQTVTPLDELEKELKLMCTILPTLLHHGGIFSVRPDLPVVPWRVRRVRVPQQSSATDCEIFCVRFFKYDATKSNMDTLT
Homology
BLAST of Moc11g30770 vs. NCBI nr
Match: XP_022158807.1 (uncharacterized protein LOC111025273 [Momordica charantia])

HSP 1 Score: 269.2 bits (687), Expect = 3.6e-68
Identity = 129/227 (56.83%), Postives = 158/227 (69.60%), Query Frame = 0

Query: 58  MDDPKTDKRLLSTATGFQKKEWYRDLLDPRVELKDEVLDGLILFTAKKLEKCLHLCHKKF 117
           MDDP TD    ST+ G + K W+  LLDP+ +L DE +D L++ TA+K+EKC HL   +F
Sbjct: 1   MDDPSTDDNSRSTSVGIKYKSWFGLLLDPQFQLDDEYIDSLMMLTARKVEKCKHLLRVQF 60

Query: 118 AIGDVLLSTLLNRTDNPYAAMKPGVLSTRINYPWSEENTIFRYVHGR------------- 177
           AIGDVLLS LL RTD PYAAMKPGVL ++  Y W +E TIFRYV GR             
Sbjct: 61  AIGDVLLSNLLRRTDGPYAAMKPGVLPSKCTYDWRQERTIFRYVLGRQSDYDTLWSEADI 120

Query: 178 ---------NHSVMLGIDLVQGDITVWDSLQTVTPLDELEKELKLMCTILPTLLHHGGIF 237
                    NH VM+GIDLV+GD+TVWDSLQ +TPL++LEK LK MCTI+P +LH  GI 
Sbjct: 121 VYTRMNIGGNHWVMIGIDLVEGDLTVWDSLQAITPLEDLEKALKPMCTIIPAILHWSGIL 180

Query: 238 SVRPDLPVVPWRVRRVRVPQQSSATDCEIFCVRFFKYDATKSNMDTL 263
           ++RP+LP+VPWRVRR  VPQQ+  TDC IFCVRFF+YD   S +DTL
Sbjct: 181 ALRPNLPMVPWRVRRCTVPQQAGFTDCSIFCVRFFEYDVIGSKIDTL 227

BLAST of Moc11g30770 vs. NCBI nr
Match: XP_022148137.1 (uncharacterized protein LOC111016890 [Momordica charantia])

HSP 1 Score: 226.9 bits (577), Expect = 2.1e-55
Identity = 114/148 (77.03%), Postives = 117/148 (79.05%), Query Frame = 0

Query: 138 MKPGVLSTRINYPWSEENTIFRYVHGR----------------------NHSVMLGIDLV 197
           MKP VLSTRINYPW EENTI+RYVHGR                      NH VMLGIDLV
Sbjct: 1   MKPXVLSTRINYPWXEENTIWRYVHGRQSDHNVPWSDADIVYTPMNVGGNHWVMLGIDLV 60

Query: 198 QGDITVWDSLQTVTPLDELEKELKLMCTILPTLLHHGGIFSVRPDLPVVPWRVRRVRVPQ 257
           QGDITVWDSLQT TPLDELEKELK MCTILPTLLHHGGIFSVRPDLPVVPWRVRRVRVPQ
Sbjct: 61  QGDITVWDSLQTATPLDELEKELKPMCTILPTLLHHGGIFSVRPDLPVVPWRVRRVRVPQ 120

Query: 258 QSSATDCEIFCVRFFKYDATKSNMDTLT 264
           QSS TDC IF VR+F+YDAT SNMDTLT
Sbjct: 121 QSSVTDCGIFYVRYFEYDATGSNMDTLT 148

BLAST of Moc11g30770 vs. NCBI nr
Match: XP_022155476.1 (uncharacterized protein LOC111022607 [Momordica charantia])

HSP 1 Score: 214.2 bits (544), Expect = 1.4e-51
Identity = 101/130 (77.69%), Postives = 109/130 (83.85%), Query Frame = 0

Query: 35  IIVDSYDPVCPITPQLDDKFQRWMDDPKTDKRLLSTATGFQKKEWYRDLLDPRVELKDEV 94
           I VD+YDP CPI PQLD +FQ WMDD   D R  STA G Q KEWYRDLLDP V+LKDEV
Sbjct: 144 ITVDAYDPACPIPPQLDGQFQTWMDDLDIDGRTRSTAAGLQGKEWYRDLLDPTVQLKDEV 203

Query: 95  LDGLILFTAKKLEKCLHLCHKKFAIGDVLLSTLLNRTDNPYAAMKPGVLSTRINYPWSEE 154
           +D L+LFTAKKLEKC++LC KKFAIGDVLLSTLLNRTD PYAAMKPGVLSTRI YP S+E
Sbjct: 204 VDALVLFTAKKLEKCIYLCRKKFAIGDVLLSTLLNRTDGPYAAMKPGVLSTRIEYPCSQE 263

Query: 155 NTIFRYVHGR 165
           NTIFRYV GR
Sbjct: 264 NTIFRYVFGR 273

BLAST of Moc11g30770 vs. NCBI nr
Match: XP_022153247.1 (uncharacterized protein LOC111020782 [Momordica charantia])

HSP 1 Score: 158.7 bits (400), Expect = 6.9e-35
Identity = 77/136 (56.62%), Postives = 91/136 (66.91%), Query Frame = 0

Query: 149 YPWSEENTIFRYVHGR----------------------NHSVMLGIDLVQGDITVWDSLQ 208
           Y W +E TIFRYV GR                      NH VM+GIDLV+GD+TVWDSLQ
Sbjct: 2   YDWRQERTIFRYVLGRQSDYDTPWSEADIVYTPMNIGGNHWVMIGIDLVEGDLTVWDSLQ 61

Query: 209 TVTPLDELEKELKLMCTILPTLLHHGGIFSVRPDLPVVPWRVRRVRVPQQSSATDCEIFC 263
            +TPL++LEK LK MCTI+P +LH  G+ ++RP L  VPWRVRR  VPQQ+  TDC IFC
Sbjct: 62  AITPLEDLEKALKPMCTIIPVILHWSGMLALRPILHTVPWRVRRCTVPQQAGFTDCGIFC 121

BLAST of Moc11g30770 vs. NCBI nr
Match: XP_022154364.1 (uncharacterized protein LOC111021646 [Momordica charantia])

HSP 1 Score: 137.5 bits (345), Expect = 1.7e-28
Identity = 72/191 (37.70%), Postives = 107/191 (56.02%), Query Frame = 0

Query: 100 LFTAKKLEKCLHLCHKKFAIGDVLLSTLLNRTDNPYAAMK-PGVLSTRI--NYPWSEEN- 159
           +F   KL+   +LC +KF  GDVL+S  L  TD  Y  M+ P V+++R+  +Y W     
Sbjct: 1   MFVCNKLKLRPNLCRRKFTTGDVLISNFLRSTDGVYVMMQSPNVIASRVASDYDWEGRAW 60

Query: 160 TIFRYVHGRN----------------------HSVMLGIDLVQGDITVWDSLQTVTPLDE 219
           ++  Y+ G +                      H +++ ID  +G++ VWDS   +TPL +
Sbjct: 61  SMLSYIDGTHSDNDTRWMDVDAVYLPYNIGGVHWIVICIDFDEGELIVWDSFMNMTPLPQ 120

Query: 220 LEKELKLMCTILPTLLHHGGIFSVRPDLPVVPWRVRRV-RVPQQSSATDCEIFCVRFFKY 264
           LE+ELK M TI+PTL+   G+   +P++P+ PWR+RRV   PQQ    DC IFC+ FF+Y
Sbjct: 121 LEQELKPMITIIPTLICRVGVHLYKPNIPLTPWRIRRVSSAPQQGMDGDCGIFCINFFEY 180

BLAST of Moc11g30770 vs. ExPASy TrEMBL
Match: A0A6J1DY60 (uncharacterized protein LOC111025273 OS=Momordica charantia OX=3673 GN=LOC111025273 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 1.8e-68
Identity = 129/227 (56.83%), Postives = 158/227 (69.60%), Query Frame = 0

Query: 58  MDDPKTDKRLLSTATGFQKKEWYRDLLDPRVELKDEVLDGLILFTAKKLEKCLHLCHKKF 117
           MDDP TD    ST+ G + K W+  LLDP+ +L DE +D L++ TA+K+EKC HL   +F
Sbjct: 1   MDDPSTDDNSRSTSVGIKYKSWFGLLLDPQFQLDDEYIDSLMMLTARKVEKCKHLLRVQF 60

Query: 118 AIGDVLLSTLLNRTDNPYAAMKPGVLSTRINYPWSEENTIFRYVHGR------------- 177
           AIGDVLLS LL RTD PYAAMKPGVL ++  Y W +E TIFRYV GR             
Sbjct: 61  AIGDVLLSNLLRRTDGPYAAMKPGVLPSKCTYDWRQERTIFRYVLGRQSDYDTLWSEADI 120

Query: 178 ---------NHSVMLGIDLVQGDITVWDSLQTVTPLDELEKELKLMCTILPTLLHHGGIF 237
                    NH VM+GIDLV+GD+TVWDSLQ +TPL++LEK LK MCTI+P +LH  GI 
Sbjct: 121 VYTRMNIGGNHWVMIGIDLVEGDLTVWDSLQAITPLEDLEKALKPMCTIIPAILHWSGIL 180

Query: 238 SVRPDLPVVPWRVRRVRVPQQSSATDCEIFCVRFFKYDATKSNMDTL 263
           ++RP+LP+VPWRVRR  VPQQ+  TDC IFCVRFF+YD   S +DTL
Sbjct: 181 ALRPNLPMVPWRVRRCTVPQQAGFTDCSIFCVRFFEYDVIGSKIDTL 227

BLAST of Moc11g30770 vs. ExPASy TrEMBL
Match: A0A6J1D492 (uncharacterized protein LOC111016890 OS=Momordica charantia OX=3673 GN=LOC111016890 PE=3 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 1.0e-55
Identity = 114/148 (77.03%), Postives = 117/148 (79.05%), Query Frame = 0

Query: 138 MKPGVLSTRINYPWSEENTIFRYVHGR----------------------NHSVMLGIDLV 197
           MKP VLSTRINYPW EENTI+RYVHGR                      NH VMLGIDLV
Sbjct: 1   MKPXVLSTRINYPWXEENTIWRYVHGRQSDHNVPWSDADIVYTPMNVGGNHWVMLGIDLV 60

Query: 198 QGDITVWDSLQTVTPLDELEKELKLMCTILPTLLHHGGIFSVRPDLPVVPWRVRRVRVPQ 257
           QGDITVWDSLQT TPLDELEKELK MCTILPTLLHHGGIFSVRPDLPVVPWRVRRVRVPQ
Sbjct: 61  QGDITVWDSLQTATPLDELEKELKPMCTILPTLLHHGGIFSVRPDLPVVPWRVRRVRVPQ 120

Query: 258 QSSATDCEIFCVRFFKYDATKSNMDTLT 264
           QSS TDC IF VR+F+YDAT SNMDTLT
Sbjct: 121 QSSVTDCGIFYVRYFEYDATGSNMDTLT 148

BLAST of Moc11g30770 vs. ExPASy TrEMBL
Match: A0A6J1DRS0 (uncharacterized protein LOC111022607 OS=Momordica charantia OX=3673 GN=LOC111022607 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 6.7e-52
Identity = 101/130 (77.69%), Postives = 109/130 (83.85%), Query Frame = 0

Query: 35  IIVDSYDPVCPITPQLDDKFQRWMDDPKTDKRLLSTATGFQKKEWYRDLLDPRVELKDEV 94
           I VD+YDP CPI PQLD +FQ WMDD   D R  STA G Q KEWYRDLLDP V+LKDEV
Sbjct: 144 ITVDAYDPACPIPPQLDGQFQTWMDDLDIDGRTRSTAAGLQGKEWYRDLLDPTVQLKDEV 203

Query: 95  LDGLILFTAKKLEKCLHLCHKKFAIGDVLLSTLLNRTDNPYAAMKPGVLSTRINYPWSEE 154
           +D L+LFTAKKLEKC++LC KKFAIGDVLLSTLLNRTD PYAAMKPGVLSTRI YP S+E
Sbjct: 204 VDALVLFTAKKLEKCIYLCRKKFAIGDVLLSTLLNRTDGPYAAMKPGVLSTRIEYPCSQE 263

Query: 155 NTIFRYVHGR 165
           NTIFRYV GR
Sbjct: 264 NTIFRYVFGR 273

BLAST of Moc11g30770 vs. ExPASy TrEMBL
Match: A0A6J1DID7 (uncharacterized protein LOC111020782 OS=Momordica charantia OX=3673 GN=LOC111020782 PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 3.4e-35
Identity = 77/136 (56.62%), Postives = 91/136 (66.91%), Query Frame = 0

Query: 149 YPWSEENTIFRYVHGR----------------------NHSVMLGIDLVQGDITVWDSLQ 208
           Y W +E TIFRYV GR                      NH VM+GIDLV+GD+TVWDSLQ
Sbjct: 2   YDWRQERTIFRYVLGRQSDYDTPWSEADIVYTPMNIGGNHWVMIGIDLVEGDLTVWDSLQ 61

Query: 209 TVTPLDELEKELKLMCTILPTLLHHGGIFSVRPDLPVVPWRVRRVRVPQQSSATDCEIFC 263
            +TPL++LEK LK MCTI+P +LH  G+ ++RP L  VPWRVRR  VPQQ+  TDC IFC
Sbjct: 62  AITPLEDLEKALKPMCTIIPVILHWSGMLALRPILHTVPWRVRRCTVPQQAGFTDCGIFC 121

BLAST of Moc11g30770 vs. ExPASy TrEMBL
Match: A0A6J1DLV0 (uncharacterized protein LOC111021646 OS=Momordica charantia OX=3673 GN=LOC111021646 PE=3 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 8.0e-29
Identity = 72/191 (37.70%), Postives = 107/191 (56.02%), Query Frame = 0

Query: 100 LFTAKKLEKCLHLCHKKFAIGDVLLSTLLNRTDNPYAAMK-PGVLSTRI--NYPWSEEN- 159
           +F   KL+   +LC +KF  GDVL+S  L  TD  Y  M+ P V+++R+  +Y W     
Sbjct: 1   MFVCNKLKLRPNLCRRKFTTGDVLISNFLRSTDGVYVMMQSPNVIASRVASDYDWEGRAW 60

Query: 160 TIFRYVHGRN----------------------HSVMLGIDLVQGDITVWDSLQTVTPLDE 219
           ++  Y+ G +                      H +++ ID  +G++ VWDS   +TPL +
Sbjct: 61  SMLSYIDGTHSDNDTRWMDVDAVYLPYNIGGVHWIVICIDFDEGELIVWDSFMNMTPLPQ 120

Query: 220 LEKELKLMCTILPTLLHHGGIFSVRPDLPVVPWRVRRV-RVPQQSSATDCEIFCVRFFKY 264
           LE+ELK M TI+PTL+   G+   +P++P+ PWR+RRV   PQQ    DC IFC+ FF+Y
Sbjct: 121 LEQELKPMITIIPTLICRVGVHLYKPNIPLTPWRIRRVSSAPQQGMDGDCGIFCINFFEY 180

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158807.13.6e-6856.83uncharacterized protein LOC111025273 [Momordica charantia][more]
XP_022148137.12.1e-5577.03uncharacterized protein LOC111016890 [Momordica charantia][more]
XP_022155476.11.4e-5177.69uncharacterized protein LOC111022607 [Momordica charantia][more]
XP_022153247.16.9e-3556.62uncharacterized protein LOC111020782 [Momordica charantia][more]
XP_022154364.11.7e-2837.70uncharacterized protein LOC111021646 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DY601.8e-6856.83uncharacterized protein LOC111025273 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A6J1D4921.0e-5577.03uncharacterized protein LOC111016890 OS=Momordica charantia OX=3673 GN=LOC111016... [more]
A0A6J1DRS06.7e-5277.69uncharacterized protein LOC111022607 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1DID73.4e-3556.62uncharacterized protein LOC111020782 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A6J1DLV08.0e-2937.70uncharacterized protein LOC111021646 OS=Momordica charantia OX=3673 GN=LOC111021... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.395.10Adenoviral Proteinase; Chain Acoord: 33..262
e-value: 5.0E-13
score: 50.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
IPR003653Ulp1 protease family, C-terminal catalytic domainPFAMPF02902Peptidase_C48coord: 155..252
e-value: 2.4E-9
score: 37.3
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 42..252

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc11g30770.1Moc11g30770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008234 cysteine-type peptidase activity