Moc11g29460 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc11g29460
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionsuccinate dehydrogenase assembly factor 1, mitochondrial
Locationchr11: 21488532 .. 21488807 (-)
RNA-Seq ExpressionMoc11g29460
SyntenyMoc11g29460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGTGAGGCCGAAGCTTTCGGGGCTGCAAAAGCAGGTGCTCAGTCTCTACAGAGGATTTCTACGAGCGGCGCGCTCCAAATCCGCCGAAGATCGGCGGCAGATGGAATCGCTCGTCGCCGCCGAGTTTCGCCGGAACGCCGACCAAATCGATCCCAAAAACTTCATCTACATCGAGTACCTCCTCCGCCGAGGTAACAAACAGCTGGATCAGCTCAAAAGCCCGGCCACTGTTCGATTGTCGTCGATGAATGTCTCAAACGCCAGATCCTGA

mRNA sequence

ATGGGTGTGAGGCCGAAGCTTTCGGGGCTGCAAAAGCAGGTGCTCAGTCTCTACAGAGGATTTCTACGAGCGGCGCGCTCCAAATCCGCCGAAGATCGGCGGCAGATGGAATCGCTCGTCGCCGCCGAGTTTCGCCGGAACGCCGACCAAATCGATCCCAAAAACTTCATCTACATCGAGTACCTCCTCCGCCGAGGTAACAAACAGCTGGATCAGCTCAAAAGCCCGGCCACTGTTCGATTGTCGTCGATGAATGTCTCAAACGCCAGATCCTGA

Coding sequence (CDS)

ATGGGTGTGAGGCCGAAGCTTTCGGGGCTGCAAAAGCAGGTGCTCAGTCTCTACAGAGGATTTCTACGAGCGGCGCGCTCCAAATCCGCCGAAGATCGGCGGCAGATGGAATCGCTCGTCGCCGCCGAGTTTCGCCGGAACGCCGACCAAATCGATCCCAAAAACTTCATCTACATCGAGTACCTCCTCCGCCGAGGTAACAAACAGCTGGATCAGCTCAAAAGCCCGGCCACTGTTCGATTGTCGTCGATGAATGTCTCAAACGCCAGATCCTGA

Protein sequence

MGVRPKLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIEYLLRRGNKQLDQLKSPATVRLSSMNVSNARS
Homology
BLAST of Moc11g29460 vs. NCBI nr
Match: XP_022141880.1 (succinate dehydrogenase assembly factor 1, mitochondrial [Momordica charantia])

HSP 1 Score: 173.3 bits (438), Expect = 9.4e-40
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 0

Query: 1  MGVRPKLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIE 60
          MGVRPKLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIE
Sbjct: 1  MGVRPKLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIE 60

Query: 61 YLLRRGNKQLDQLKSPATVRLSSMNVSNARS 92
          YLLRRGNKQLDQLKSPATVRLSSMNVSNARS
Sbjct: 61 YLLRRGNKQLDQLKSPATVRLSSMNVSNARS 91

BLAST of Moc11g29460 vs. NCBI nr
Match: XP_038999431.1 (succinate dehydrogenase assembly factor 1, mitochondrial-like [Hibiscus syriacus] >XP_038999436.1 succinate dehydrogenase assembly factor 1, mitochondrial-like [Hibiscus syriacus])

HSP 1 Score: 135.2 bits (339), Expect = 2.8e-28
Identity = 68/86 (79.07%), Postives = 81/86 (94.19%), Query Frame = 0

Query: 6  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIEYLLRR 65
          KLSG+QKQVLSLYRGFLRAARSK AEDRRQ+ES+V+AEFRRN++Q+D KNF+YIEYLLRR
Sbjct: 8  KLSGMQKQVLSLYRGFLRAARSKPAEDRRQIESIVSAEFRRNSNQVDRKNFLYIEYLLRR 67

Query: 66 GNKQLDQLKSPATVRLSSMNVSNARS 92
          GNKQLDQLKSP T+ LSS+NVS+ ++
Sbjct: 68 GNKQLDQLKSPDTIGLSSVNVSSYKN 93

BLAST of Moc11g29460 vs. NCBI nr
Match: XP_008459591.1 (PREDICTED: succinate dehydrogenase assembly factor 1, mitochondrial [Cucumis melo])

HSP 1 Score: 135.2 bits (339), Expect = 2.8e-28
Identity = 70/80 (87.50%), Postives = 76/80 (95.00%), Query Frame = 0

Query: 6  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIEYLLRR 65
          KLSGLQKQVLSLYR FLRAAR+KS EDR+QMES+VAAEFRRNA QID KNFIYIEYLLRR
Sbjct: 3  KLSGLQKQVLSLYRAFLRAARTKSVEDRQQMESIVAAEFRRNAKQIDRKNFIYIEYLLRR 62

Query: 66 GNKQLDQLKSPATVRLSSMN 86
          GNKQLDQLKSPATVRLS+++
Sbjct: 63 GNKQLDQLKSPATVRLSTLD 82

BLAST of Moc11g29460 vs. NCBI nr
Match: XP_038890134.1 (succinate dehydrogenase assembly factor 1, mitochondrial [Benincasa hispida])

HSP 1 Score: 134.4 bits (337), Expect = 4.9e-28
Identity = 70/80 (87.50%), Postives = 75/80 (93.75%), Query Frame = 0

Query: 6  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIEYLLRR 65
          KLSGLQKQVLSLYR FLRAARSKSAEDRRQ+ES+VAAEFRRNA QID KNFI+IEYLLRR
Sbjct: 3  KLSGLQKQVLSLYRAFLRAARSKSAEDRRQIESIVAAEFRRNAKQIDRKNFIHIEYLLRR 62

Query: 66 GNKQLDQLKSPATVRLSSMN 86
          GNKQLDQLKSP TVR S++N
Sbjct: 63 GNKQLDQLKSPGTVRFSTIN 82

BLAST of Moc11g29460 vs. NCBI nr
Match: XP_011656085.1 (succinate dehydrogenase assembly factor 1, mitochondrial [Cucumis sativus] >KGN52665.1 hypothetical protein Csa_008140 [Cucumis sativus])

HSP 1 Score: 133.7 bits (335), Expect = 8.3e-28
Identity = 69/80 (86.25%), Postives = 75/80 (93.75%), Query Frame = 0

Query: 6  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIEYLLRR 65
          KLSGLQKQVLSLYR FLRAAR+KS EDR+QMES+VA EFRRNA QID KNFIYIEYLLRR
Sbjct: 3  KLSGLQKQVLSLYRAFLRAARTKSVEDRQQMESIVATEFRRNAKQIDRKNFIYIEYLLRR 62

Query: 66 GNKQLDQLKSPATVRLSSMN 86
          GNKQLDQLKSPATVRLS+++
Sbjct: 63 GNKQLDQLKSPATVRLSTLD 82

BLAST of Moc11g29460 vs. ExPASy Swiss-Prot
Match: B0K036 (Succinate dehydrogenase assembly factor 1, mitochondrial OS=Rattus norvegicus OX=10116 GN=Sdhaf1 PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.4e-06
Identity = 34/70 (48.57%), Postives = 44/70 (62.86%), Query Frame = 0

Query: 6  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIEYLLRR 65
          +LS LQ+QVLSLYR  LRA R K   + R     V AEFR++A  +   + + IEYL RR
Sbjct: 3  RLSRLQRQVLSLYRELLRAGRGKPGAEAR-----VRAEFRQHA-SLPRSDVLRIEYLYRR 62

Query: 66 GNKQLDQLKS 76
          G +QL  L+S
Sbjct: 63 GRRQLQLLRS 66

BLAST of Moc11g29460 vs. ExPASy Swiss-Prot
Match: Q9US02 (Succinate dehydrogenase assembly factor 1, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC664.12c PE=3 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 7.1e-06
Identity = 29/70 (41.43%), Postives = 41/70 (58.57%), Query Frame = 0

Query: 7  LSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIEYLLRRG 66
          LSGLQ+QV+  YR  L AA++K      +  + V  EFR+N   I  ++F YIE+LLR G
Sbjct: 3  LSGLQRQVIHFYRRCLHAAKAKEQPYNERWMAFVHQEFRKN-QTISKRDFFYIEHLLRVG 62

Query: 67 NKQLDQLKSP 77
           +Q +    P
Sbjct: 63 QRQYEAYSRP 71

BLAST of Moc11g29460 vs. ExPASy Swiss-Prot
Match: A6NFY7 (Succinate dehydrogenase assembly factor 1, mitochondrial OS=Homo sapiens OX=9606 GN=SDHAF1 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 1.2e-05
Identity = 33/68 (48.53%), Postives = 42/68 (61.76%), Query Frame = 0

Query: 8  SGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIEYLLRRGN 67
          S LQ+QVLSLYR  LRA R K   + R     V AEFR++A  +   + + IEYL RRG 
Sbjct: 5  SRLQRQVLSLYRDLLRAGRGKPGAEAR-----VRAEFRQHAG-LPRSDVLRIEYLYRRGR 64

Query: 68 KQLDQLKS 76
          +QL  L+S
Sbjct: 65 RQLQLLRS 66

BLAST of Moc11g29460 vs. ExPASy Swiss-Prot
Match: Q54HG5 (Succinate dehydrogenase assembly factor 1A, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=sdhaf1A PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 1.2e-05
Identity = 28/70 (40.00%), Postives = 42/70 (60.00%), Query Frame = 0

Query: 8  SGLQKQVLSLYRGFLRAARSKSAED-RRQMESLVAAEFRRNADQIDPKNFIYIEYLLRRG 67
          SGLQK VLSLYR FLR +  K  ++    + + +  +FR  A  I  ++   IE LL +G
Sbjct: 9  SGLQKAVLSLYRSFLRVSNKKEMDNPSSSISNYIKTQFRLKATTIQKRDINKIESLLVKG 68

Query: 68 NKQLDQLKSP 77
           +QL+Q++ P
Sbjct: 69 KRQLEQIQDP 78

BLAST of Moc11g29460 vs. ExPASy Swiss-Prot
Match: A8PU71 (Succinate dehydrogenase assembly factor 1, mitochondrial OS=Bos taurus OX=9913 GN=SDHAF1 PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.6e-05
Identity = 33/68 (48.53%), Postives = 42/68 (61.76%), Query Frame = 0

Query: 8  SGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIEYLLRRGN 67
          S LQ+QVLSLYR  LRA R K   + R     V AEFR++A  +   + + IEYL RRG 
Sbjct: 5  SRLQRQVLSLYRELLRAGRGKPGAEAR-----VRAEFRQHA-CLPRSDVLRIEYLYRRGR 64

Query: 68 KQLDQLKS 76
          +QL  L+S
Sbjct: 65 RQLQMLRS 66

BLAST of Moc11g29460 vs. ExPASy TrEMBL
Match: A0A6J1CJC1 (succinate dehydrogenase assembly factor 1, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111012137 PE=4 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 4.6e-40
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 0

Query: 1  MGVRPKLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIE 60
          MGVRPKLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIE
Sbjct: 1  MGVRPKLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIE 60

Query: 61 YLLRRGNKQLDQLKSPATVRLSSMNVSNARS 92
          YLLRRGNKQLDQLKSPATVRLSSMNVSNARS
Sbjct: 61 YLLRRGNKQLDQLKSPATVRLSSMNVSNARS 91

BLAST of Moc11g29460 vs. ExPASy TrEMBL
Match: A0A1S3CAJ2 (succinate dehydrogenase assembly factor 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103498675 PE=4 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.4e-28
Identity = 70/80 (87.50%), Postives = 76/80 (95.00%), Query Frame = 0

Query: 6  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIEYLLRR 65
          KLSGLQKQVLSLYR FLRAAR+KS EDR+QMES+VAAEFRRNA QID KNFIYIEYLLRR
Sbjct: 3  KLSGLQKQVLSLYRAFLRAARTKSVEDRQQMESIVAAEFRRNAKQIDRKNFIYIEYLLRR 62

Query: 66 GNKQLDQLKSPATVRLSSMN 86
          GNKQLDQLKSPATVRLS+++
Sbjct: 63 GNKQLDQLKSPATVRLSTLD 82

BLAST of Moc11g29460 vs. ExPASy TrEMBL
Match: A0A0A0KST3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G649345 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 4.0e-28
Identity = 69/80 (86.25%), Postives = 75/80 (93.75%), Query Frame = 0

Query: 6  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIEYLLRR 65
          KLSGLQKQVLSLYR FLRAAR+KS EDR+QMES+VA EFRRNA QID KNFIYIEYLLRR
Sbjct: 3  KLSGLQKQVLSLYRAFLRAARTKSVEDRQQMESIVATEFRRNAKQIDRKNFIYIEYLLRR 62

Query: 66 GNKQLDQLKSPATVRLSSMN 86
          GNKQLDQLKSPATVRLS+++
Sbjct: 63 GNKQLDQLKSPATVRLSTLD 82

BLAST of Moc11g29460 vs. ExPASy TrEMBL
Match: A0A7N2LDJ5 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 8.9e-28
Identity = 67/83 (80.72%), Postives = 76/83 (91.57%), Query Frame = 0

Query: 5  PKLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIEYLLR 64
          PKLSG+QKQVLSLYRGFLRAARSKSAEDR ++ES+V+ EFRRN+ Q+D KNF+YIEYLLR
Sbjct: 7  PKLSGMQKQVLSLYRGFLRAARSKSAEDRHKIESIVSGEFRRNSKQVDRKNFLYIEYLLR 66

Query: 65 RGNKQLDQLKSPATVRLSSMNVS 88
          RG KQLDQLKSP TV LSS+NVS
Sbjct: 67 RGQKQLDQLKSPDTVGLSSLNVS 89

BLAST of Moc11g29460 vs. ExPASy TrEMBL
Match: A0A6N2M6N5 (Uncharacterized protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS325582 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 9.9e-27
Identity = 67/86 (77.91%), Postives = 75/86 (87.21%), Query Frame = 0

Query: 2  GVRPKLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIEY 61
          G R +LSG+QKQVLSLYRGFLRAARSK  EDRRQ+ES V+AEFRRN+ Q+D KNFIYIEY
Sbjct: 6  GGRARLSGMQKQVLSLYRGFLRAARSKPPEDRRQIESFVSAEFRRNSKQVDRKNFIYIEY 65

Query: 62 LLRRGNKQLDQLKSPATVRLSSMNVS 88
          LLRRG KQL+QL SP TV LSSMNV+
Sbjct: 66 LLRRGKKQLEQLNSPDTVGLSSMNVT 91

BLAST of Moc11g29460 vs. TAIR 10
Match: AT2G39725.1 (LYR family of Fe/S cluster biogenesis protein )

HSP 1 Score: 112.1 bits (279), Expect = 2.4e-25
Identity = 57/86 (66.28%), Postives = 71/86 (82.56%), Query Frame = 0

Query: 6  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIEYLLRR 65
          KLSG+QKQVLSLYRGFLRAARS+  EDR+++E +V+ EFR N+ ++D KNF YIEYLLR 
Sbjct: 5  KLSGMQKQVLSLYRGFLRAARSRPIEDRKRIEMIVSTEFRHNSKEVDRKNFQYIEYLLRL 64

Query: 66 GNKQLDQLKSPATVRLSSMNVSNARS 92
          G KQLDQLKSPA V LSS+ V  +++
Sbjct: 65 GTKQLDQLKSPAIVSLSSIKVVTSKT 90

BLAST of Moc11g29460 vs. TAIR 10
Match: AT2G39725.2 (LYR family of Fe/S cluster biogenesis protein )

HSP 1 Score: 112.1 bits (279), Expect = 2.4e-25
Identity = 57/86 (66.28%), Postives = 71/86 (82.56%), Query Frame = 0

Query: 6  KLSGLQKQVLSLYRGFLRAARSKSAEDRRQMESLVAAEFRRNADQIDPKNFIYIEYLLRR 65
          KLSG+QKQVLSLYRGFLRAARS+  EDR+++E +V+ EFR N+ ++D KNF YIEYLLR 
Sbjct: 5  KLSGMQKQVLSLYRGFLRAARSRPIEDRKRIEMIVSTEFRHNSKEVDRKNFQYIEYLLRL 64

Query: 66 GNKQLDQLKSPATVRLSSMNVSNARS 92
          G KQLDQLKSPA V LSS+ V  +++
Sbjct: 65 GTKQLDQLKSPAIVSLSSIKVVTSKT 90

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141880.19.4e-40100.00succinate dehydrogenase assembly factor 1, mitochondrial [Momordica charantia][more]
XP_038999431.12.8e-2879.07succinate dehydrogenase assembly factor 1, mitochondrial-like [Hibiscus syriacus... [more]
XP_008459591.12.8e-2887.50PREDICTED: succinate dehydrogenase assembly factor 1, mitochondrial [Cucumis mel... [more]
XP_038890134.14.9e-2887.50succinate dehydrogenase assembly factor 1, mitochondrial [Benincasa hispida][more]
XP_011656085.18.3e-2886.25succinate dehydrogenase assembly factor 1, mitochondrial [Cucumis sativus] >KGN5... [more]
Match NameE-valueIdentityDescription
B0K0362.4e-0648.57Succinate dehydrogenase assembly factor 1, mitochondrial OS=Rattus norvegicus OX... [more]
Q9US027.1e-0641.43Succinate dehydrogenase assembly factor 1, mitochondrial OS=Schizosaccharomyces ... [more]
A6NFY71.2e-0548.53Succinate dehydrogenase assembly factor 1, mitochondrial OS=Homo sapiens OX=9606... [more]
Q54HG51.2e-0540.00Succinate dehydrogenase assembly factor 1A, mitochondrial OS=Dictyostelium disco... [more]
A8PU711.6e-0548.53Succinate dehydrogenase assembly factor 1, mitochondrial OS=Bos taurus OX=9913 G... [more]
Match NameE-valueIdentityDescription
A0A6J1CJC14.6e-40100.00succinate dehydrogenase assembly factor 1, mitochondrial OS=Momordica charantia ... [more]
A0A1S3CAJ21.4e-2887.50succinate dehydrogenase assembly factor 1, mitochondrial OS=Cucumis melo OX=3656... [more]
A0A0A0KST34.0e-2886.25Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G649345 PE=4 SV=1[more]
A0A7N2LDJ58.9e-2880.72Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A6N2M6N59.9e-2777.91Uncharacterized protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS325582 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT2G39725.12.4e-2566.28LYR family of Fe/S cluster biogenesis protein [more]
AT2G39725.22.4e-2566.28LYR family of Fe/S cluster biogenesis protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008011Complex 1 LYR proteinPFAMPF05347Complex1_LYRcoord: 12..72
e-value: 2.7E-13
score: 49.7
NoneNo IPR availablePANTHERPTHR13675:SF1SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 1, MITOCHONDRIALcoord: 6..85
NoneNo IPR availablePANTHERPTHR13675LYR MOTIF-CONTAINING PROTEIN 2coord: 6..85
NoneNo IPR availableCDDcd20268Complex1_LYR_SDHAF1_LYRM8coord: 8..72
e-value: 1.99507E-25
score: 87.6518

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc11g29460.1Moc11g29460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034553 mitochondrial respiratory chain complex II assembly
cellular_component GO:0005739 mitochondrion