Moc11g28830 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc11g28830
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRibonuclease H
Locationchr11: 21013188 .. 21015362 (-)
RNA-Seq ExpressionMoc11g28830
SyntenyMoc11g28830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGATCCAACCCTGAGGAGGCAAGTGACTTATCCCTAGCTAACTATTTTCTCAACTCTCCCTCAATAACTTAAGCATCAGAGCCCGTGTGACTAAGGTCACACCGGTTTAGATCGGTTTGATTTTGCAGGTATCGGTTCCTTTATCATCGAGTTAGGTGTATGCGAGAAGAAGAGCCGGATGGTCAAAATCCATGCATCAACAATTGGCGCCGTCTATGGGGACGATACAGTAAAGCAAGCTTCGTTGAAGCGAAATCCCGATCCGAGTGAAATTCGGATCCGAGGCGTGTAGAGTGAAACGGCCGACCATTCGGGAGAGGTAGGACTGAGCCCTGATCATCGACTTGATCTGAAAGCGACATGAATCCTGATGAGAGCCATCAATGCTTCGAAAAGAAGAGCCTGAAAGAAGCGGCCGATCACTCGGATGAGGCAAGACCGAGCCCTGATCATCGCCTTAATCCGACCACCCTTGACAGGGGAGAATCAGCGTAGGGCTTGAATGAAGCGACAGACCTCTCGGGTGAGGCAAGGCCGAGCCCTGGTCATTGACTTAATCTGACCACCCTTAACAAGGGAGAACCAGTGACGAGCCTGAATAAAGTGACAGACCTCTCGGGTGAGGCAAGGTCGAGCCCTGGTCATCGACTTAATCCAACCACCCTAGATAGGGGAGAACCAGTGACGAGCCTGAATGAAGCGAACGACCTCTCGGGTGAGGTAACGCCGAACCCTGGTCATCGACTTAATTCGACCACCCTCGACAGGGGAGAACCGGCTAAGGAGGTAGAATCTGTCCCTCTGACAGCCGAAGATCGACGGGTCAACATTGGAACCAGGTTGGGGGATGAGGAAAGAGAAAGCCTTATAAACTTCCTCAGAAGCAACACCGACGGTGTTCGCATGGTCGCCGGAAGACGTGTCGGGAATCGAGCGAGGAGTCATGGTACGTCGACTCAATGTCGACGAGAGCTGTAAGCTAGTGAAACATAAGCGTCACGCGTTCAACCAAGAAAGGAACGAGATTGTAGCTGAACAGGTGGAGGAGCTACTAAAAGCAGACTTCATTCGGGAAGTACATTATCCCCAGTGGATATCCAACGTAGTGCTGGTAAAGAAGCCAAACGACAAATGGAGAATGTGCATCGACTTCACGGACCTAAATAAGGCATGTCCAAAGGATTCTTTTCCTTTGCCTCGAATAGATCAACTGGTTGATGCCACTATAGGACATGAAAGATTGAGTTTCATGGATGCCCAACTCCCTTCAATATCTATTAGCTTGGTTCCCTACCAACTCTGTTTTCTAAATCCAAAACTGGATGCCAGGCGACTTTGATAAAACACTTTGCCTCGTCTACAAACATACTCTCAAAGGATATCGACAATACAATACCTTGAGAGTAGGGGCAATAGGTCATGACCGACTCAGCGCGCAAGCCTGGTAAAACCAGCCCGAGCAGAGCAGGTCGAGCAGGGCTCGGGTCCCACCCAGCCGAGCACATCTCTGCTCGGATCCGACCACTGCTCGGCACCGACCAGGTGCAATGAGGCATAAATCTACATTAAAGACATATTTTGAAAGCAGAATCTACATTAATGGACAAATTTGGAAAGAACAATTAAACAATCTCAACACGGCCAAACCAGAAACGGGTCGATTGACCTAGCACAGTTAAAACATGAGATGGAGTAAAAACGACAGTGGGTATAAAACCAGTGGCTATAGTCAAAGGATTAGAAACCTATGAAAATGAAAGCAACAAAGTCAACTCCATCAACGAAGCCGAATATGAAGCTCTACTAGCAGGACTAAAAGTGGCCAGGTACGTTGGGGTCAGGAACATATTGATACTAAGTGATTCACAACTGGTTGTAGGTCAGGTGAAACAGGAGTTCCAGGCTAGAGAACCGCGAATGGCGAAATACCTTTCACAGGTAAAAAACCAGCTCGACCAGTTCTCAAAATATGAGATTCGACAAATTCTACGTGCTCAAAACGTCAACGCCGATGCGTTAGCTCGACTAGCTGCAACTTATGAAACCGACCTAGGCAGAACTGTACCGATCGAGATTCTACCCGAGCCAAGCATAGAAGCTCAGGAAGCAATGAATATCGACGAGCAAGGGCAACCAGAAGAAAACTGGAAGAGCCCTTTGATCAATATTTGA

mRNA sequence

ATGAGATCCAACCCTGAGGAGGCAAGTATCGGTTCCTTTATCATCGAGTTAGGTGTATGCGAGAAGAAGAGCCGGATGGTCAAAATCCATGCATCAACAATTGGCGCCGTCTATGGGGACGATACAGTAAAGCAAGCTTCGTTGAAGCGAAATCCCGATCCGAAACCAGTGACGAGCCTGAATAAAGTGACAGACCTCTCGGGTGAGGCAAGGTCGAGCCCTGGTCATCGACTTAATCCAACCACCCTAGATAGGGGAGAACCAGTGACGAGCCTGAATGAAGCGAACGACCTCTCGGGTGAGGTAACGCCGAACCCTGGTCATCGACTTAATTCGACCACCCTCGACAGGGGAGAACCGGCTAAGGAGGTCATGACCGACTCAGCGCGCAAGCCTGGTAAAACCAGCCCGAGCAGAGCAGGTCGAGCAGGGCTCGGGTCCCACCCAGCCGAGCACATCTCTGCTCGGATCCGACCACTGCTCGGCACCGACCAGGTGAAACAGGAGTTCCAGGCTAGAGAACCGCGAATGGCGAAATACCTTTCACAGGTAAAAAACCAGCTCGACCAGTTCTCAAAATATGAGATTCGACAAATTCTACGTGCTCAAAACGTCAACGCCGATGCGTTAGCTCGACTAGCTGCAACTTATGAAACCGACCTAGGCAGAACTGTACCGATCGAGATTCTACCCGAGCCAAGCATAGAAGCTCAGGAAGCAATGAATATCGACGAGCAAGGGCAACCAGAAGAAAACTGGAAGAGCCCTTTGATCAATATTTGA

Coding sequence (CDS)

ATGAGATCCAACCCTGAGGAGGCAAGTATCGGTTCCTTTATCATCGAGTTAGGTGTATGCGAGAAGAAGAGCCGGATGGTCAAAATCCATGCATCAACAATTGGCGCCGTCTATGGGGACGATACAGTAAAGCAAGCTTCGTTGAAGCGAAATCCCGATCCGAAACCAGTGACGAGCCTGAATAAAGTGACAGACCTCTCGGGTGAGGCAAGGTCGAGCCCTGGTCATCGACTTAATCCAACCACCCTAGATAGGGGAGAACCAGTGACGAGCCTGAATGAAGCGAACGACCTCTCGGGTGAGGTAACGCCGAACCCTGGTCATCGACTTAATTCGACCACCCTCGACAGGGGAGAACCGGCTAAGGAGGTCATGACCGACTCAGCGCGCAAGCCTGGTAAAACCAGCCCGAGCAGAGCAGGTCGAGCAGGGCTCGGGTCCCACCCAGCCGAGCACATCTCTGCTCGGATCCGACCACTGCTCGGCACCGACCAGGTGAAACAGGAGTTCCAGGCTAGAGAACCGCGAATGGCGAAATACCTTTCACAGGTAAAAAACCAGCTCGACCAGTTCTCAAAATATGAGATTCGACAAATTCTACGTGCTCAAAACGTCAACGCCGATGCGTTAGCTCGACTAGCTGCAACTTATGAAACCGACCTAGGCAGAACTGTACCGATCGAGATTCTACCCGAGCCAAGCATAGAAGCTCAGGAAGCAATGAATATCGACGAGCAAGGGCAACCAGAAGAAAACTGGAAGAGCCCTTTGATCAATATTTGA

Protein sequence

MRSNPEEASIGSFIIELGVCEKKSRMVKIHASTIGAVYGDDTVKQASLKRNPDPKPVTSLNKVTDLSGEARSSPGHRLNPTTLDRGEPVTSLNEANDLSGEVTPNPGHRLNSTTLDRGEPAKEVMTDSARKPGKTSPSRAGRAGLGSHPAEHISARIRPLLGTDQVKQEFQAREPRMAKYLSQVKNQLDQFSKYEIRQILRAQNVNADALARLAATYETDLGRTVPIEILPEPSIEAQEAMNIDEQGQPEENWKSPLINI
Homology
[Close]
BLAST of Moc11g28830 vs. NCBI nr
Match: XP_022156571.1 (uncharacterized protein LOC111023448 [Momordica charantia])

HSP 1 Score: 161.0 bits (406), Expect = 1.4e-35
Identity = 83/94 (88.30%), Postives = 88/94 (93.62%), Query Frame = 0 

Query: 165 QVKQEFQAREPRMAKYLSQVKNQLDQFSKYEIRQILRAQNVNADALARLAATYETDLGRT 224
           QVK+EFQAREPRMAKYLSQV+NQLDQFSKYEI QI RAQNV+ADALARLAA YETDLGRT
Sbjct: 726 QVKEEFQAREPRMAKYLSQVRNQLDQFSKYEIXQIPRAQNVBADALARLAAXYETDLGRT 785

Query: 225 VPIEILPEPSIEAQEAMNIDEQGQPEENWKSPLI 259
           VP+EILPEPSIEAQEAM+ID QGQ EEN KSPLI
Sbjct: 786 VPVEILPEPSIEAQEAMDIDNQGQXEENXKSPLI 819

[Close]
BLAST of Moc11g28830 vs. NCBI nr
Match: XP_022156571.1 (uncharacterized protein LOC111023448 [Momordica charantia])

HSP 1 Score: 106.7 bits (265), Expect = 3.1e-19
Identity = 51/70 (72.86%), Postives = 60/70 (85.71%), Query Frame = 0 

Query: 55  KPVTSLNKVTDLSGEARSSPGHRLNPTTLDRGEPVTSLNEANDLSGEVTPNPGHRLNSTT 114
           +PV+ LNK TDLSG+AR SPG+RLNPTTLD+G PV  L +A DLSG+  P+PGHRLNSTT
Sbjct: 42  EPVSGLNKATDLSGKARPSPGYRLNPTTLDKGGPVAGLIKATDLSGDARPSPGHRLNSTT 101

Query: 115 LDRGEPAKEV 125
           LDRGEPA+EV
Sbjct: 102 LDRGEPAEEV 111


HSP 2 Score: 159.1 bits (401), Expect = 5.3e-35
Identity = 81/94 (86.17%), Postives = 86/94 (91.49%), Query Frame = 0 

Query: 165 QVKQEFQAREPRMAKYLSQVKNQLDQFSKYEIRQILRAQNVNADALARLAATYETDLGRT 224
           QVK++FQARE RMAKYLSQV+NQLDQFSKYEIRQI  AQNVNAD LARLA  YETDLGRT
Sbjct: 389 QVKEKFQARELRMAKYLSQVRNQLDQFSKYEIRQIPHAQNVNADVLARLAVAYETDLGRT 448

Query: 225 VPIEILPEPSIEAQEAMNIDEQGQPEENWKSPLI 259
           VP+EILP PSIEAQEAM+ID QGQPEENWKSPLI
Sbjct: 449 VPVEILPGPSIEAQEAMDIDNQGQPEENWKSPLI 482

[Close]
BLAST of Moc11g28830 vs. NCBI nr
Match: XP_022154103.1 (uncharacterized protein LOC111021439 [Momordica charantia])

HSP 1 Score: 158.3 bits (399), Expect = 9.0e-35
Identity = 82/94 (87.23%), Postives = 87/94 (92.55%), Query Frame = 0 

Query: 165 QVKQEFQAREPRMAKYLSQVKNQLDQFSKYEIRQILRAQNVNADALARLAATYETDLGRT 224
           QVK+EFQARE RM KYLSQV+NQLDQFSKYEIRQI RAQNVNADALARLA TYETDLGRT
Sbjct: 182 QVKEEFQARESRMTKYLSQVRNQLDQFSKYEIRQIPRAQNVNADALARLATTYETDLGRT 241

Query: 225 VPIEILPEPSIEAQEAMNIDEQGQPEENWKSPLI 259
           VP+EIL  PSIEAQEAM+ID+QGQPEENWKS LI
Sbjct: 242 VPVEILLGPSIEAQEAMDIDDQGQPEENWKSLLI 275

[Close]
BLAST of Moc11g28830 vs. NCBI nr
Match: XP_022159204.1 (uncharacterized protein LOC111025623 [Momordica charantia])

HSP 1 Score: 137.5 bits (345), Expect = 1.6e-28
Identity = 73/94 (77.66%), Postives = 81/94 (86.17%), Query Frame = 0 

Query: 165 QVKQEFQAREPRMAKYLSQVKNQLDQFSKYEIRQILRAQNVNADALARLAATYETDLGRT 224
           QVK+EFQAREPRMAKYLS+VK+QL QFSK+EIRQI   QNVNADALARLAA Y TDLGRT
Sbjct: 329 QVKEEFQAREPRMAKYLSRVKDQLGQFSKFEIRQIPCTQNVNADALARLAAAYGTDLGRT 388

Query: 225 VPIEILPEPSIEAQEAMNIDEQGQPEENWKSPLI 259
           + +EIL EPSI+AQE M+IDEQ Q EENW S LI
Sbjct: 389 ISVEILLEPSIKAQEKMDIDEQRQSEENWMSHLI 422

[Close]
BLAST of Moc11g28830 vs. NCBI nr
Match: XP_022153142.1 (uncharacterized protein LOC111020710 [Momordica charantia])

HSP 1 Score: 103.6 bits (257), Expect = 2.6e-18
Identity = 52/109 (47.71%), Postives = 73/109 (66.97%), Query Frame = 0 

Query: 150 AEHISARIRPLLGTDQVKQEFQAREPRMAKYLSQVKNQLDQFSKYEIRQILRAQNVNADA 209
           A HI       L  +Q+K+E+QA++PRM KYLS+V++ L QF  YE+ Q+ R++N NADA
Sbjct: 104 ASHIKVFXDSXLIVNQIKEEYQAKDPRMEKYLSKVRSHLAQFGTYEVSQVPRSENSNADA 163

Query: 210 LARLAATYETDLGRTVPIEILPEPSIEAQEAMNIDEQGQPEENWKSPLI 259
           LA+LA+ YETDL R+VP+EIL  PSI   + M +D    P   W  P++
Sbjct: 164 LAKLASAYETDLARSVPVEILDTPSILEPDVMEVD---TPSLTWMDPIV 209

[Close]
BLAST of Moc11g28830 vs. ExPASy TrEMBL
Match: A0A6J1DVC6 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023448 PE=4 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 1.5e-35
Identity = 83/94 (88.30%), Postives = 88/94 (93.62%), Query Frame = 0 

Query: 165 QVKQEFQAREPRMAKYLSQVKNQLDQFSKYEIRQILRAQNVNADALARLAATYETDLGRT 224
           QVK+EFQAREPRMAKYLSQV+NQLDQFSKYEI QI RAQNV+ADALARLAA YETDLGRT
Sbjct: 726 QVKEEFQAREPRMAKYLSQVRNQLDQFSKYEIXQIPRAQNVDADALARLAAXYETDLGRT 785

Query: 225 VPIEILPEPSIEAQEAMNIDEQGQPEENWKSPLI 259
           VP+EILPEPSIEAQEAM+ID QGQ EEN KSPLI
Sbjct: 786 VPVEILPEPSIEAQEAMDIDNQGQXEENXKSPLI 819

[Close]
BLAST of Moc11g28830 vs. ExPASy TrEMBL
Match: A0A6J1DVC6 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023448 PE=4 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 1.5e-19
Identity = 51/70 (72.86%), Postives = 60/70 (85.71%), Query Frame = 0 

Query: 55  KPVTSLNKVTDLSGEARSSPGHRLNPTTLDRGEPVTSLNEANDLSGEVTPNPGHRLNSTT 114
           +PV+ LNK TDLSG+AR SPG+RLNPTTLD+G PV  L +A DLSG+  P+PGHRLNSTT
Sbjct: 42  EPVSGLNKATDLSGKARPSPGYRLNPTTLDKGGPVAGLIKATDLSGDARPSPGHRLNSTT 101

Query: 115 LDRGEPAKEV 125
           LDRGEPA+EV
Sbjct: 102 LDRGEPAEEV 111


HSP 2 Score: 159.1 bits (401), Expect = 2.5e-35
Identity = 81/94 (86.17%), Postives = 86/94 (91.49%), Query Frame = 0 

Query: 165 QVKQEFQAREPRMAKYLSQVKNQLDQFSKYEIRQILRAQNVNADALARLAATYETDLGRT 224
           QVK++FQARE RMAKYLSQV+NQLDQFSKYEIRQI  AQNVNAD LARLA  YETDLGRT
Sbjct: 389 QVKEKFQARELRMAKYLSQVRNQLDQFSKYEIRQIPHAQNVNADVLARLAVAYETDLGRT 448

Query: 225 VPIEILPEPSIEAQEAMNIDEQGQPEENWKSPLI 259
           VP+EILP PSIEAQEAM+ID QGQPEENWKSPLI
Sbjct: 449 VPVEILPGPSIEAQEAMDIDNQGQPEENWKSPLI 482

[Close]
BLAST of Moc11g28830 vs. ExPASy TrEMBL
Match: A0A6J1DJC7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111021439 PE=4 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 4.3e-35
Identity = 82/94 (87.23%), Postives = 87/94 (92.55%), Query Frame = 0 

Query: 165 QVKQEFQAREPRMAKYLSQVKNQLDQFSKYEIRQILRAQNVNADALARLAATYETDLGRT 224
           QVK+EFQARE RM KYLSQV+NQLDQFSKYEIRQI RAQNVNADALARLA TYETDLGRT
Sbjct: 182 QVKEEFQARESRMTKYLSQVRNQLDQFSKYEIRQIPRAQNVNADALARLATTYETDLGRT 241

Query: 225 VPIEILPEPSIEAQEAMNIDEQGQPEENWKSPLI 259
           VP+EIL  PSIEAQEAM+ID+QGQPEENWKS LI
Sbjct: 242 VPVEILLGPSIEAQEAMDIDDQGQPEENWKSLLI 275

[Close]
BLAST of Moc11g28830 vs. ExPASy TrEMBL
Match: A0A6J1E1Q5 (uncharacterized protein LOC111025623 OS=Momordica charantia OX=3673 GN=LOC111025623 PE=4 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 7.9e-29
Identity = 73/94 (77.66%), Postives = 81/94 (86.17%), Query Frame = 0 

Query: 165 QVKQEFQAREPRMAKYLSQVKNQLDQFSKYEIRQILRAQNVNADALARLAATYETDLGRT 224
           QVK+EFQAREPRMAKYLS+VK+QL QFSK+EIRQI   QNVNADALARLAA Y TDLGRT
Sbjct: 329 QVKEEFQAREPRMAKYLSRVKDQLGQFSKFEIRQIPCTQNVNADALARLAAAYGTDLGRT 388

Query: 225 VPIEILPEPSIEAQEAMNIDEQGQPEENWKSPLI 259
           + +EIL EPSI+AQE M+IDEQ Q EENW S LI
Sbjct: 389 ISVEILLEPSIKAQEKMDIDEQRQSEENWMSHLI 422

[Close]
BLAST of Moc11g28830 vs. ExPASy TrEMBL
Match: A0A6J1E3S9 (uncharacterized protein LOC111025810 OS=Momordica charantia OX=3673 GN=LOC111025810 PE=4 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 9.7e-19
Identity = 55/115 (47.83%), Postives = 76/115 (66.09%), Query Frame = 0 

Query: 144 GLGSHPAEHISARIRPLLGTDQVKQEFQAREPRMAKYLSQVKNQLDQFSKYEIRQILRAQ 203
           GLG   A HI       L  +Q+K+E+Q ++PRM KYLS+V++ L QF  YE+ Q+ R++
Sbjct: 283 GLG---ANHIKVFSDSQLIVNQIKEEYQTKDPRMEKYLSKVRSHLAQFGTYEVXQVPRSE 342

Query: 204 NVNADALARLAATYETDLGRTVPIEILPEPSIEAQEAMNIDEQGQPEENWKSPLI 259
           N NADALA+LA+ YETDL R+VPIEIL  PSI   + M +D    P  +W  P++
Sbjct: 343 NSNADALAKLASAYETDLARSVPIEILDNPSILEPDVMEVD---TPSPSWMDPIV 391

The following BLAST results are available for this feature:
BLAST of Moc11g28830 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022156571.11.4e-3588.30uncharacterized protein LOC111023448 [Momordica charantia][more]
XP_022156571.13.1e-1972.86uncharacterized protein LOC111023448 [Momordica charantia][more]
XP_022154103.19.0e-3587.23uncharacterized protein LOC111021439 [Momordica charantia][more]
XP_022159204.11.6e-2877.66uncharacterized protein LOC111025623 [Momordica charantia][more]
XP_022153142.12.6e-1847.71uncharacterized protein LOC111020710 [Momordica charantia][more]
BLAST of Moc11g28830 vs. NCBI nr
Match NameE-valueIdentityDescription
BLAST of Moc11g28830 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1DVC61.5e-3588.30Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023448 PE=4 SV=1[more]
A0A6J1DVC61.5e-1972.86Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023448 PE=4 SV=1[more]
A0A6J1DJC74.3e-3587.23Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111021439 PE=4 SV=1[more]
A0A6J1E1Q57.9e-2977.66uncharacterized protein LOC111025623 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A6J1E3S99.7e-1947.83uncharacterized protein LOC111025810 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
BLAST of Moc11g28830 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 160..225
e-value: 3.4E-7
score: 32.1
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 164..214
e-value: 3.1E-5
score: 23.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 47..156
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 52..112

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc11g28830.1Moc11g28830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0030430 host cell cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity