Moc11g14050 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc11g14050
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ribonuclease H
Location	chr11: 10439589 .. 10441356 (+)
RNA-Seq Expression	Moc11g14050
Synteny	Moc11g14050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGCCTCGCCGGGATGACCGACCTGCGGTCATCAACACTATTTTCGGAGGTCCGAGCGGGGACCAGTCTGGAAACAAGAGGAAAGAGCTAGCTCGCGAGGCCAGGCACGAGGTATGCATCATCAGGGAGCAGAAACCTACTTGCTCCATCACTTTCGGTGATGCCGATCTAGAGGGGATCCATTTGCCCTATAATGACACGCTCGTGATCGCCCCTCTCATTGACCACGTCCTGGTCCGAAGAGTATTGGTTGATGGAGGCGCATCTGCCAACATCTTGTCCCTCTCAACATATCTAGCATTGGGATGAACCAGGTCACAATTGAAGAAGAGTCCAACACCCTTGGTTGGATTCTCTGGAGAATCGGTCTCCCCTGAAGGGTGCATCGACCTGCTGGTAACTATCGGGCAAGATGCTACCCAAGTAACGCAGATGGCCGAGTTCGTAGTGATCGACGGCAGATCGGCCTATAATGTCATTTTCGGGAGACCCATCATCCACTCATTTCGGGCCGTCCCCTCCACACTGCATCAAGTCTTGAAATACTCAACCCCGAATGGGGTGGGCACGGTCCGAGGTGAGCAAAAAACCTCACGGGAGTGTTATGCATCCGCGCTTAAAGGGTCGGCAGTATGCGCCCTGGAAGAGCAAACCAGTCGTGGCAAGCTGCAGGAGTTAGAGGCCGACCTGCCAAAGGAAGGCAAAAGGCAGTTCTCCCCGCCAACAGAAGAGCTCGAGCTTGTTCCTCTACTTAGCCCTGAAAAACAAGTAAGCATAGGAACCAAACTGGGGGCCACTGACAGGGAAAAACTGATCAACTTCCTCAGGTCTAACTCAGACGTCTTCGCATGGTCTCACGAGGACATGTCTGGTATCGACCCAAAGATCATGGTGCATCGTCTCAACGTAGACCCATCATTCCGACCTGTAAAGAAAAAAAGAAGACCTGTAAATAAAGAGAGGAATGATGTAATTGTTGAGGAAGTAAACAAACTCTTGAAAGCTGAATATATACGAGAAATTTTGTATCCTGAGTGGCTCTCTAACGTTGTATTAGTTAAAAAATCCAACGGGAGGTGGAGGATGTGCGTGGACTTAACAAATTTAAATAAGGCGTGCCCAAAAGATTGCTTCCCCCTTCCAAGGATTGACCAGCTCGTTGACGCAACAGCCGGGCACGAGCTACTCACCTTCATGGATGCCTACTCCGGATACAACCAAATTAAAATGCATTTGTCAAACCAAGATCATAAAGCGATGACTCAAGCTAAGGTTGGACTCCAGAAAGGGGAGATGGGCCGAGGAGCTACCTAAGGTTCTGTGGTCATATCGAACCACCCCGCGGGAGTCGACTGGCGAAACTCCGTTCTCGCTAGCCTTTGGTTCCGAAGCTGTTGTACCAGTCGAGATCGGCATACCAACAGACAGGGTAGAACAGTACGAGTCAACAAAGAACGAGGAAGAGCTACTCTTTAACCTGGACCTATTGGAAGGGAAAAGGGAAATGGCTCAGCTACGCTTAGCAGAGTATCAGAACAGAATGGCAAGACATTACAATGCCCGAGTTCGACCTCGAAGCTTCCAAGTTGGACATTTGGTCTTGAGAAAAATTCAGAGTCATGTTGGCACCCTTGACCCAAGTTGGAAGGGACCGTTCGAAGTCAAAGGCATAGTCCGACCTGGAACTTATATGCTGGCAGACCTAGAGGGAAGAGTGCTTGCGCATCCATGGAACGCGGAGCACTTGAAGCGCTATTACCCCTGA

mRNA sequence

ATGCCGCCTCGCCGGGATGACCGACCTGCGGTCATCAACACTATTTTCGGAGGTCCGAGCGGGGACCAGTCTGGAAACAAGAGGAAAGAGCTAGCTCGCGAGGCCAGGCACGAGGTATGCATCATCAGGGAGCAGAAACCTACTTGCTCCATCACTTTCGGTGATGCCGATCTAGAGGGGATCCATTTGCCCTATAATGACACGCTCGTGATCGCCCCTCTCATTGACCACGTCCTGGTCACAATTGAAGAAGAGTCCAACACCCTTGGTTGGATTCTCTGGAGAATCGGGTCGGCAGTATGCGCCCTGGAAGAGCAAACCAGTCGTGGCAAGCTGCAGGAGTTAGAGGCCGACCTGCCAAAGGAAGGCAAAAGGCAGTTCTCCCCGCCAACAGAAGAGCTCGAGCTTGTTCCTCTACTTAGCCCTGAAAAACAATACGAGTCAACAAAGAACGAGGAAGAGCTACTCTTTAACCTGGACCTATTGGAAGGGAAAAGGGAAATGGCTCAGCTACGCTTAGCAGAGTATCAGAACAGAATGGCAAGACATTACAATGCCCGAGTTCGACCTCGAAGCTTCCAAGTTGGACATTTGGTCTTGAGAAAAATTCAGAGTCATGTTGGCACCCTTGACCCAAGTTGGAAGGGACCGTTCGAAGTCAAAGGCATAGTCCGACCTGGAACTTATATGCTGGCAGACCTAGAGGGAAGAGTGCTTGCGCATCCATGGAACGCGGAGCACTTGAAGCGCTATTACCCCTGA

Coding sequence (CDS)

ATGCCGCCTCGCCGGGATGACCGACCTGCGGTCATCAACACTATTTTCGGAGGTCCGAGCGGGGACCAGTCTGGAAACAAGAGGAAAGAGCTAGCTCGCGAGGCCAGGCACGAGGTATGCATCATCAGGGAGCAGAAACCTACTTGCTCCATCACTTTCGGTGATGCCGATCTAGAGGGGATCCATTTGCCCTATAATGACACGCTCGTGATCGCCCCTCTCATTGACCACGTCCTGGTCACAATTGAAGAAGAGTCCAACACCCTTGGTTGGATTCTCTGGAGAATCGGGTCGGCAGTATGCGCCCTGGAAGAGCAAACCAGTCGTGGCAAGCTGCAGGAGTTAGAGGCCGACCTGCCAAAGGAAGGCAAAAGGCAGTTCTCCCCGCCAACAGAAGAGCTCGAGCTTGTTCCTCTACTTAGCCCTGAAAAACAATACGAGTCAACAAAGAACGAGGAAGAGCTACTCTTTAACCTGGACCTATTGGAAGGGAAAAGGGAAATGGCTCAGCTACGCTTAGCAGAGTATCAGAACAGAATGGCAAGACATTACAATGCCCGAGTTCGACCTCGAAGCTTCCAAGTTGGACATTTGGTCTTGAGAAAAATTCAGAGTCATGTTGGCACCCTTGACCCAAGTTGGAAGGGACCGTTCGAAGTCAAAGGCATAGTCCGACCTGGAACTTATATGCTGGCAGACCTAGAGGGAAGAGTGCTTGCGCATCCATGGAACGCGGAGCACTTGAAGCGCTATTACCCCTGA

Protein sequence

MPPRRDDRPAVINTIFGGPSGDQSGNKRKELAREARHEVCIIREQKPTCSITFGDADLEGIHLPYNDTLVIAPLIDHVLVTIEEESNTLGWILWRIGSAVCALEEQTSRGKLQELEADLPKEGKRQFSPPTEELELVPLLSPEKQYESTKNEEELLFNLDLLEGKREMAQLRLAEYQNRMARHYNARVRPRSFQVGHLVLRKIQSHVGTLDPSWKGPFEVKGIVRPGTYMLADLEGRVLAHPWNAEHLKRYYP

Homology

[Close]

BLAST of Moc11g14050 vs. NCBI nr
Match: XP_022158414.1 (uncharacterized protein LOC111024904 [Momordica charantia])

HSP 1 Score: 198.4 bits (503), Expect = 7.6e-47
Identity = 124/243 (51.03%), Postives = 142/243 (58.44%), Query Frame = 0


Query: 2   PPRRDDRPAVINTIFGGPSGDQSGNKRKELAREARHEVCIIREQKPTCSITFGDADLEGI 61
           PPRR+DRPAVINTIFGGP+G QSGNKRKELAREAR EVCIIRE KPTCSITFGDADLEG+
Sbjct: 289 PPRREDRPAVINTIFGGPNGGQSGNKRKELAREARREVCIIREHKPTCSITFGDADLEGV 348

Query: 62  HLPYNDTLVIAPLIDHVL--------------VTIEEES--------------------- 121
           HLP+ND LVIA LIDH L              VTI +++                     
Sbjct: 349 HLPHNDALVIASLIDHDLVRRVLIDGGCIDLPVTIGQDATQVTQMAEFVVIDGRSAYNAI 408

Query: 122 -------------------------NTLGWILWR------------IGSAVCALEEQTSR 173
                                    N +G +                GSAVCALEEQT+R
Sbjct: 409 FGRPIIHSFRAVPSTLHQVLKYSTPNEVGMVRGEQKTSRECYASALKGSAVCALEEQTNR 468

[Close]

BLAST of Moc11g14050 vs. NCBI nr
Match: XP_022159003.1 (uncharacterized protein LOC111025448 [Momordica charantia])

HSP 1 Score: 187.6 bits (475), Expect = 1.3e-43
Identity = 98/138 (71.01%), Postives = 105/138 (76.09%), Query Frame = 0


Query: 116 EADLPKEGKRQFSPPTEELELVPLLSPEKQYESTKNEEELLFNLDLLEGKREMAQLRLAE 175
           EA +P E       PT+ +E         QYE  +NE ELL NLDLLEGKRE AQLRL E
Sbjct: 505 EAMVPAE----IGMPTDRIE---------QYEPLRNEAELLLNLDLLEGKRETAQLRLVE 564

Query: 176 YQNRMARHYNARVRPRSFQVGHLVLRKIQSHVGTLDPSWKGPFEVKGIVRPGTYMLADLE 235
            QNRMARHYN R++PRSFQVGHLV  KIQSHVGTLDPSW+  FEVKGIVRPGTYMLADLE
Sbjct: 565 CQNRMARHYNTRIQPRSFQVGHLVSSKIQSHVGTLDPSWEELFEVKGIVRPGTYMLADLE 624

Query: 236 GRVLAHPWNAEHLKRYYP 254
             VLAHPWNAEHLKRYYP
Sbjct: 625 RSVLAHPWNAEHLKRYYP 629

[Close]

BLAST of Moc11g14050 vs. NCBI nr
Match: XP_022154846.1 (uncharacterized protein LOC111022006 [Momordica charantia])

HSP 1 Score: 174.9 bits (442), Expect = 9.0e-40
Identity = 117/274 (42.70%), Postives = 131/274 (47.81%), Query Frame = 0


Query: 2   PPRRDDRPAVINTIFGGPSGDQSGNKRKELAREARHEVCIIREQKPTCSITFGDADLEGI 61
           PPRRDDRPAVINTIFGGPSG Q GNKR +LAR  R EVCIIREQKPTC ITFGDADLEG+
Sbjct: 180 PPRRDDRPAVINTIFGGPSGGQFGNKRNKLARGTRREVCIIREQKPTCPITFGDADLEGV 239

Query: 62  HLPYNDTLVIAPLIDHVL---VTIEEESN----------TLGWI---------------- 121
           HLP+ND LVIAPLIDH+L   V I+  ++           LGW                 
Sbjct: 240 HLPHNDALVIAPLIDHILVRRVLIDGGASANIWSLPTYLALGWTRSQLKKSPTPLVGFSG 299

Query: 122 ------------------------------------------------LWRI-------- 158
                                                            W +        
Sbjct: 300 ESVSPEGCIDLSVTIGQDATQVTQMAEFVVIDDKSAYNAIFGRPIIHSFWAVSSTLHQVL 359

[Close]

BLAST of Moc11g14050 vs. NCBI nr
Match: XP_022154405.1 (uncharacterized protein LOC111021682 [Momordica charantia])

HSP 1 Score: 164.1 bits (414), Expect = 1.6e-36
Identity = 103/214 (48.13%), Postives = 116/214 (54.21%), Query Frame = 0


Query: 3   PRRDDRPAVINTIFGGPSGDQSGNKRKELAREARHEVCIIREQKPTCSITFGDADLEGIH 62
           PRRDDRP +IN IFGGP+G QS NKRKELAREA+ EVCIIREQKPTCSITFGDADLEG+H
Sbjct: 195 PRRDDRPTIINIIFGGPNGGQSRNKRKELAREAKREVCIIREQKPTCSITFGDADLEGVH 254

Query: 63  LPYNDTLVIAPLIDHVLV------------------------------------------ 122
           LP+ND LVIAPLIDHVLV                                          
Sbjct: 255 LPHNDALVIAPLIDHVLVRRVLVYGGCIDLPVTIGQDDTQVTQMAEFVVIDGRSAYNAIF 314

Query: 123 ----------------------------TIEEESNTLG--WILWRIGSAVCALEEQTSRG 145
                                       T+  E  TL   +     GS+VCAL  Q +  
Sbjct: 315 GRPIIHSFRVVPSTVHQVLKYSTPNGVGTVRGEQKTLRECYASALKGSSVCAL-GQANGD 374

[Close]

BLAST of Moc11g14050 vs. NCBI nr
Match: XP_022156070.1 (uncharacterized protein K02A2.6-like [Momordica charantia])

HSP 1 Score: 159.1 bits (401), Expect = 5.1e-35
Identity = 78/113 (69.03%), Postives = 92/113 (81.42%), Query Frame = 0


Query: 130 PTEELELVPLLSPEKQYESTKNEEELLFNLDLLEGKREMAQLRLAEYQNRMARHYNARVR 189
           PTE +E          YE  +NEEELL  LDL++ KRE AQLRLAEYQN+MARHYN+RVR
Sbjct: 247 PTERVE---------YYERVRNEEELLLYLDLIDEKREAAQLRLAEYQNQMARHYNSRVR 306

Query: 190 PRSFQVGHLVLRKIQSHVGTLDPSWKGPFEVKGIVRPGTYMLADLEGRVLAHP 243
            RSFQ+G+LVLRK+Q HV  L+PSW+GPFEVKGIVRPGTYMLADLEG++L+HP
Sbjct: 307 SRSFQIGYLVLRKVQKHVEVLEPSWEGPFEVKGIVRPGTYMLADLEGKLLSHP 350

[Close]

BLAST of Moc11g14050 vs. ExPASy TrEMBL
Match: A0A6J1DZB9 (uncharacterized protein LOC111024904 OS=Momordica charantia OX=3673 GN=LOC111024904 PE=4 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 3.7e-47
Identity = 124/243 (51.03%), Postives = 142/243 (58.44%), Query Frame = 0


Query: 2   PPRRDDRPAVINTIFGGPSGDQSGNKRKELAREARHEVCIIREQKPTCSITFGDADLEGI 61
           PPRR+DRPAVINTIFGGP+G QSGNKRKELAREAR EVCIIRE KPTCSITFGDADLEG+
Sbjct: 289 PPRREDRPAVINTIFGGPNGGQSGNKRKELAREARREVCIIREHKPTCSITFGDADLEGV 348

Query: 62  HLPYNDTLVIAPLIDHVL--------------VTIEEES--------------------- 121
           HLP+ND LVIA LIDH L              VTI +++                     
Sbjct: 349 HLPHNDALVIASLIDHDLVRRVLIDGGCIDLPVTIGQDATQVTQMAEFVVIDGRSAYNAI 408

Query: 122 -------------------------NTLGWILWR------------IGSAVCALEEQTSR 173
                                    N +G +                GSAVCALEEQT+R
Sbjct: 409 FGRPIIHSFRAVPSTLHQVLKYSTPNEVGMVRGEQKTSRECYASALKGSAVCALEEQTNR 468

[Close]

BLAST of Moc11g14050 vs. ExPASy TrEMBL
Match: A0A6J1DXL9 (uncharacterized protein LOC111025448 OS=Momordica charantia OX=3673 GN=LOC111025448 PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 6.5e-44
Identity = 98/138 (71.01%), Postives = 105/138 (76.09%), Query Frame = 0


Query: 116 EADLPKEGKRQFSPPTEELELVPLLSPEKQYESTKNEEELLFNLDLLEGKREMAQLRLAE 175
           EA +P E       PT+ +E         QYE  +NE ELL NLDLLEGKRE AQLRL E
Sbjct: 505 EAMVPAE----IGMPTDRIE---------QYEPLRNEAELLLNLDLLEGKRETAQLRLVE 564

Query: 176 YQNRMARHYNARVRPRSFQVGHLVLRKIQSHVGTLDPSWKGPFEVKGIVRPGTYMLADLE 235
            QNRMARHYN R++PRSFQVGHLV  KIQSHVGTLDPSW+  FEVKGIVRPGTYMLADLE
Sbjct: 565 CQNRMARHYNTRIQPRSFQVGHLVSSKIQSHVGTLDPSWEELFEVKGIVRPGTYMLADLE 624

Query: 236 GRVLAHPWNAEHLKRYYP 254
             VLAHPWNAEHLKRYYP
Sbjct: 625 RSVLAHPWNAEHLKRYYP 629

[Close]

BLAST of Moc11g14050 vs. ExPASy TrEMBL
Match: A0A6J1DPX9 (uncharacterized protein LOC111022006 OS=Momordica charantia OX=3673 GN=LOC111022006 PE=4 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 4.4e-40
Identity = 117/274 (42.70%), Postives = 131/274 (47.81%), Query Frame = 0


Query: 2   PPRRDDRPAVINTIFGGPSGDQSGNKRKELAREARHEVCIIREQKPTCSITFGDADLEGI 61
           PPRRDDRPAVINTIFGGPSG Q GNKR +LAR  R EVCIIREQKPTC ITFGDADLEG+
Sbjct: 180 PPRRDDRPAVINTIFGGPSGGQFGNKRNKLARGTRREVCIIREQKPTCPITFGDADLEGV 239

Query: 62  HLPYNDTLVIAPLIDHVL---VTIEEESN----------TLGWI---------------- 121
           HLP+ND LVIAPLIDH+L   V I+  ++           LGW                 
Sbjct: 240 HLPHNDALVIAPLIDHILVRRVLIDGGASANIWSLPTYLALGWTRSQLKKSPTPLVGFSG 299

Query: 122 ------------------------------------------------LWRI-------- 158
                                                            W +        
Sbjct: 300 ESVSPEGCIDLSVTIGQDATQVTQMAEFVVIDDKSAYNAIFGRPIIHSFWAVSSTLHQVL 359

[Close]

BLAST of Moc11g14050 vs. ExPASy TrEMBL
Match: A0A6J1DJI4 (uncharacterized protein LOC111021682 OS=Momordica charantia OX=3673 GN=LOC111021682 PE=4 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 7.7e-37
Identity = 103/214 (48.13%), Postives = 116/214 (54.21%), Query Frame = 0


Query: 3   PRRDDRPAVINTIFGGPSGDQSGNKRKELAREARHEVCIIREQKPTCSITFGDADLEGIH 62
           PRRDDRP +IN IFGGP+G QS NKRKELAREA+ EVCIIREQKPTCSITFGDADLEG+H
Sbjct: 195 PRRDDRPTIINIIFGGPNGGQSRNKRKELAREAKREVCIIREQKPTCSITFGDADLEGVH 254

Query: 63  LPYNDTLVIAPLIDHVLV------------------------------------------ 122
           LP+ND LVIAPLIDHVLV                                          
Sbjct: 255 LPHNDALVIAPLIDHVLVRRVLVYGGCIDLPVTIGQDDTQVTQMAEFVVIDGRSAYNAIF 314

Query: 123 ----------------------------TIEEESNTLG--WILWRIGSAVCALEEQTSRG 145
                                       T+  E  TL   +     GS+VCAL  Q +  
Sbjct: 315 GRPIIHSFRVVPSTVHQVLKYSTPNGVGTVRGEQKTLRECYASALKGSSVCAL-GQANGD 374

[Close]

BLAST of Moc11g14050 vs. ExPASy TrEMBL
Match: A0A6J1DP99 (uncharacterized protein K02A2.6-like OS=Momordica charantia OX=3673 GN=LOC111023037 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 2.5e-35
Identity = 78/113 (69.03%), Postives = 92/113 (81.42%), Query Frame = 0


Query: 130 PTEELELVPLLSPEKQYESTKNEEELLFNLDLLEGKREMAQLRLAEYQNRMARHYNARVR 189
           PTE +E          YE  +NEEELL  LDL++ KRE AQLRLAEYQN+MARHYN+RVR
Sbjct: 247 PTERVE---------YYERVRNEEELLLYLDLIDEKREAAQLRLAEYQNQMARHYNSRVR 306

Query: 190 PRSFQVGHLVLRKIQSHVGTLDPSWKGPFEVKGIVRPGTYMLADLEGRVLAHP 243
            RSFQ+G+LVLRK+Q HV  L+PSW+GPFEVKGIVRPGTYMLADLEG++L+HP
Sbjct: 307 SRSFQIGYLVLRKVQKHVEVLEPSWEGPFEVKGIVRPGTYMLADLEGKLLSHP 350

The following BLAST results are available for this feature:

BLAST of Moc11g14050 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158414.1	7.6e-47	51.03	uncharacterized protein LOC111024904 [Momordica charantia]	[more]
XP_022159003.1	1.3e-43	71.01	uncharacterized protein LOC111025448 [Momordica charantia]	[more]
XP_022154846.1	9.0e-40	42.70	uncharacterized protein LOC111022006 [Momordica charantia]	[more]
XP_022154405.1	1.6e-36	48.13	uncharacterized protein LOC111021682 [Momordica charantia]	[more]
XP_022156070.1	5.1e-35	69.03	uncharacterized protein K02A2.6-like [Momordica charantia]	[more]

BLAST of Moc11g14050 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc11g14050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DZB9	3.7e-47	51.03	uncharacterized protein LOC111024904 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A6J1DXL9	6.5e-44	71.01	uncharacterized protein LOC111025448 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6J1DPX9	4.4e-40	42.70	uncharacterized protein LOC111022006 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1DJI4	7.7e-37	48.13	uncharacterized protein LOC111021682 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
A0A6J1DP99	2.5e-35	69.03	uncharacterized protein K02A2.6-like OS=Momordica charantia OX=3673 GN=LOC111023...	[more]

BLAST of Moc11g14050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 159..186

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc11g14050.1	Moc11g14050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity