Moc11g13120 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc11g13120
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Retrovirus-related Pol polyprotein from transposon TNT 1-94
Location	chr11: 9724331 .. 9724708 (-)
RNA-Seq Expression	Moc11g13120
Synteny	Moc11g13120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGTTAACAAAACTTGGTCCATTGTTCATCTTCCACCTGGACAATATTCGATTGGGTGTAAGTGGGTCTATAAGGTGAAGCACAATTCCGATGGTTCGGTAGAGCACTACAAGGCTCGATTGGTTGCCAAGGGATATACACAACAAGAAGATGTTGATTTTATTGAAACCTTATCTCCAATTGCTAAATTGGTTGTTGTCAAGGTCCTTCTCACTATTGTTAAAATGCAAATGATTTGTATTAGTAATTTAATTATTATATTTTTTAAGAAAAATATGGGGATAACTAATATTGATTTAAATTACACTTTTTTTTCGGTCTCCCAATTTCGTGGTTCCATGGTAAGAAGTTCCCTTTTGCAAATTTTTAGGTGA

mRNA sequence

ATGGAAGTTAACAAAACTTGGTCCATTGTTCATCTTCCACCTGGACAATATTCGATTGGGTGTAAGTGGGTCTATAAGGTGAAGCACAATTCCGATGGTTCGGTAGAGCACTACAAGGCTCGATTGGTTGCCAAGGGATATACACAACAAGAAGATGTTGATTTTATTGAAACCTTATCTCCAATTGCTAAATTGGTTGTTGTCAAGGTCCTTCTCACTATTGTTAAAATGCAAATGATTTGTATTAGTAATTTAATTATTATATTTTTTAAGAAAAATATGGGGATAACTAATATTGATTTAAATTACACTTTTTTTTCGGTCTCCCAATTTCGTGGTTCCATGGTAAGAAGTTCCCTTTTGCAAATTTTTAGGTGA

Coding sequence (CDS)

ATGGAAGTTAACAAAACTTGGTCCATTGTTCATCTTCCACCTGGACAATATTCGATTGGGTGTAAGTGGGTCTATAAGGTGAAGCACAATTCCGATGGTTCGGTAGAGCACTACAAGGCTCGATTGGTTGCCAAGGGATATACACAACAAGAAGATGTTGATTTTATTGAAACCTTATCTCCAATTGCTAAATTGGTTGTTGTCAAGGTCCTTCTCACTATTGTTAAAATGCAAATGATTTGTATTAGTAATTTAATTATTATATTTTTTAAGAAAAATATGGGGATAACTAATATTGATTTAAATTACACTTTTTTTTCGGTCTCCCAATTTCGTGGTTCCATGGTAAGAAGTTCCCTTTTGCAAATTTTTAGGTGA

Protein sequence

MEVNKTWSIVHLPPGQYSIGCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLSPIAKLVVVKVLLTIVKMQMICISNLIIIFFKKNMGITNIDLNYTFFSVSQFRGSMVRSSLLQIFR

Homology

[Close]

BLAST of Moc11g13120 vs. NCBI nr
Match: XP_022154919.1 (uncharacterized protein LOC111022065 [Momordica charantia])

HSP 1 Score: 118.6 bits (296), Expect = 3.8e-23
Identity = 55/74 (74.32%), Postives = 63/74 (85.14%), Query Frame = 0


Query: 1   MEVNKTWSIVHLPPGQYSIGCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLS 60
           ME N TWS+V LP   +SIGCKW+YKVKH SDGS+E YKARLVAKGYTQQE +D+IET S
Sbjct: 749 MEANHTWSVVPLPYEHHSIGCKWIYKVKHKSDGSIERYKARLVAKGYTQQEGLDYIETFS 808

Query: 61  PIAKLVVVKVLLTI 75
           P+AKLV VKVLLT+
Sbjct: 809 PVAKLVTVKVLLTL 822

[Close]

BLAST of Moc11g13120 vs. NCBI nr
Match: XP_022156273.1 (uncharacterized protein LOC111023206 [Momordica charantia])

HSP 1 Score: 118.2 bits (295), Expect = 4.9e-23
Identity = 55/74 (74.32%), Postives = 63/74 (85.14%), Query Frame = 0


Query: 1  MEVNKTWSIVHLPPGQYSIGCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLS 60
          ME+NKTWS+V L P Q+SI CKWVYK+KHN DGS+EHYKA LVAKGYTQQE +DFIET S
Sbjct: 8  MEINKTWSVVPLSPRQHSIECKWVYKMKHNFDGSIEHYKAPLVAKGYTQQEGIDFIETFS 67

Query: 61 PIAKLVVVKVLLTI 75
           +AKLV VK+ LTI
Sbjct: 68 LVAKLVTVKIFLTI 81

[Close]

BLAST of Moc11g13120 vs. NCBI nr
Match: XP_022147774.1 (uncharacterized protein LOC111016631 isoform X1 [Momordica charantia] >XP_022147776.1 uncharacterized protein LOC111016631 isoform X1 [Momordica charantia])

HSP 1 Score: 117.9 bits (294), Expect = 6.5e-23
Identity = 57/75 (76.00%), Postives = 65/75 (86.67%), Query Frame = 0


Query: 1   MEVNKTWSIVHLPPGQYSIGCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLS 60
           ME N TWS+V LPPG +SIGCKWVYKVKHN DGS+E YKARLVAKGYTQQE +D+IET S
Sbjct: 219 MESNNTWSVV-LPPGHHSIGCKWVYKVKHNPDGSIERYKARLVAKGYTQQEGLDYIETFS 278

Query: 61  PIAKLVVVKVLLTIV 76
            +AKLV VK+LLT+V
Sbjct: 279 LVAKLVNVKILLTLV 292

[Close]

BLAST of Moc11g13120 vs. NCBI nr
Match: KAG7554761.1 (Retrotransposon gag domain [Arabidopsis suecica])

HSP 1 Score: 117.1 bits (292), Expect = 1.1e-22
Identity = 55/105 (52.38%), Postives = 72/105 (68.57%), Query Frame = 0


Query: 1   MEVNKTWSIVHLPPGQYSIGCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLS 60
           ME+NKTWS+V LPPG+  +GCKWVY +K+N+DGS+E YKARLVAKGYTQQE +D+ ET S
Sbjct: 681 MELNKTWSVVSLPPGKNVVGCKWVYTIKYNADGSIERYKARLVAKGYTQQEGIDYFETFS 740

Query: 61  PIAKLVVVKVLLTIVKMQMICISNLIIIFFKKNMGITNIDLNYTF 106
           P+AKL  VK++L +                KKN  +T +D+   F
Sbjct: 741 PVAKLATVKLILGLAA--------------KKNWSLTQMDVTNAF 771

[Close]

BLAST of Moc11g13120 vs. NCBI nr
Match: RVW99848.1 (Retrovirus-related Pol polyprotein from transposon RE1 [Vitis vinifera])

HSP 1 Score: 116.3 bits (290), Expect = 1.9e-22
Identity = 53/78 (67.95%), Postives = 65/78 (83.33%), Query Frame = 0


Query: 1   MEVNKTWSIVHLPPGQYSIGCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLS 60
           ME+NKTWS+V LP G++SIGCKW+YKVK  SDGS+E +KA +VAKGYTQQE +DF ET S
Sbjct: 329 MELNKTWSVVSLPKGKHSIGCKWIYKVKFKSDGSIERHKAPIVAKGYTQQEGLDFFETFS 388

Query: 61  PIAKLVVVKVLLTIVKMQ 79
           P+AKLV VKVLL +  +Q
Sbjct: 389 PVAKLVTVKVLLALATIQ 406

[Close]

BLAST of Moc11g13120 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 9.7e-14
Identity = 34/72 (47.22%), Postives = 50/72 (69.44%), Query Frame = 0


Query: 4    NKTWSIVHLPPGQYSI-GCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLSPI 63
            N TW +V  PP   +I GC+W++  K+NSDGS+  YKARLVAKGY Q+  +D+ ET SP+
Sbjct: 982  NHTWDLVPPPPSHVTIVGCRWIFTKKYNSDGSLNRYKARLVAKGYNQRPGLDYAETFSPV 1041

Query: 64   AKLVVVKVLLTI 75
             K   ++++L +
Sbjct: 1042 IKSTSIRIVLGV 1053

[Close]

BLAST of Moc11g13120 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 3.7e-13
Identity = 34/72 (47.22%), Postives = 49/72 (68.06%), Query Frame = 0


Query: 4    NKTWSIVHLPPGQYSI-GCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLSPI 63
            N TW +V  PP   +I GC+W++  K NSDGS+  YKARLVAKGY Q+  +D+ ET SP+
Sbjct: 965  NHTWDLVPPPPPSVTIVGCRWIFTKKFNSDGSLNRYKARLVAKGYNQRPGLDYAETFSPV 1024

Query: 64   AKLVVVKVLLTI 75
             K   ++++L +
Sbjct: 1025 IKSTSIRIVLGV 1036

[Close]

BLAST of Moc11g13120 vs. ExPASy Swiss-Prot
Match: P92520 (Uncharacterized mitochondrial protein AtMg00820 OS=Arabidopsis thaliana OX=3702 GN=AtMg00820 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 8.2e-13
Identity = 40/95 (42.11%), Postives = 60/95 (63.16%), Query Frame = 0


Query: 4   NKTWSIVHLPPGQYSIGCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLSPIA 63
           NKTW +V  P  Q  +GCKWV+K K +SDG+++  KARLVAKG+ Q+E + F+ET SP+ 
Sbjct: 54  NKTWILVPPPVNQNILGCKWVFKTKLHSDGTLDRLKARLVAKGFHQEEGIYFVETYSPVV 113

Query: 64  KLVVVKVLLTIVKMQMICISNLIIIFFKK--NMGI 97
           +   ++ +L +   Q + +   I   FK   +MGI
Sbjct: 114 RTATIRTILNVA--QQLEVGQSINWMFKMHFSMGI 146

[Close]

BLAST of Moc11g13120 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.6e-11
Identity = 29/74 (39.19%), Postives = 50/74 (67.57%), Query Frame = 0


Query: 1   MEVNKTWSIVHLPPGQYSIGCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLS 60
           ++ N T+ +V LP G+  + CKWV+K+K + D  +  YKARLV KG+ Q++ +DF E  S
Sbjct: 837 LQKNGTYKLVELPKGKRPLKCKWVFKLKKDGDCKLVRYKARLVVKGFEQKKGIDFDEIFS 896

Query: 61  PIAKLVVVKVLLTI 75
           P+ K+  ++ +L++
Sbjct: 897 PVVKMTSIRTILSL 910

[Close]

BLAST of Moc11g13120 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 67.0 bits (162), Expect = 1.7e-10
Identity = 28/74 (37.84%), Postives = 50/74 (67.57%), Query Frame = 0


Query: 2   EVNKTWSIVHLPPGQYSIGCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLSP 61
           ++N TW+I   P  +  +  +WV+ VK+N  G+   YKARLVA+G+TQ+  +D+ ET +P
Sbjct: 918 KINNTWTITKRPENKNIVDSRWVFSVKYNELGNPIRYKARLVARGFTQKYQIDYEETFAP 977

Query: 62  IAKLVVVKVLLTIV 76
           +A++   + +L++V
Sbjct: 978 VARISSFRFILSLV 991

[Close]

BLAST of Moc11g13120 vs. ExPASy TrEMBL
Match: A0A6J1DNP7 (uncharacterized protein LOC111022065 OS=Momordica charantia OX=3673 GN=LOC111022065 PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.8e-23
Identity = 55/74 (74.32%), Postives = 63/74 (85.14%), Query Frame = 0


Query: 1   MEVNKTWSIVHLPPGQYSIGCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLS 60
           ME N TWS+V LP   +SIGCKW+YKVKH SDGS+E YKARLVAKGYTQQE +D+IET S
Sbjct: 749 MEANHTWSVVPLPYEHHSIGCKWIYKVKHKSDGSIERYKARLVAKGYTQQEGLDYIETFS 808

Query: 61  PIAKLVVVKVLLTI 75
           P+AKLV VKVLLT+
Sbjct: 809 PVAKLVTVKVLLTL 822

[Close]

BLAST of Moc11g13120 vs. ExPASy TrEMBL
Match: A0A6J1DQ66 (uncharacterized protein LOC111023206 OS=Momordica charantia OX=3673 GN=LOC111023206 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 2.4e-23
Identity = 55/74 (74.32%), Postives = 63/74 (85.14%), Query Frame = 0


Query: 1  MEVNKTWSIVHLPPGQYSIGCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLS 60
          ME+NKTWS+V L P Q+SI CKWVYK+KHN DGS+EHYKA LVAKGYTQQE +DFIET S
Sbjct: 8  MEINKTWSVVPLSPRQHSIECKWVYKMKHNFDGSIEHYKAPLVAKGYTQQEGIDFIETFS 67

Query: 61 PIAKLVVVKVLLTI 75
           +AKLV VK+ LTI
Sbjct: 68 LVAKLVTVKIFLTI 81

[Close]

BLAST of Moc11g13120 vs. ExPASy TrEMBL
Match: A0A6J1D203 (uncharacterized protein LOC111016631 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016631 PE=4 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 3.1e-23
Identity = 57/75 (76.00%), Postives = 65/75 (86.67%), Query Frame = 0


Query: 1   MEVNKTWSIVHLPPGQYSIGCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLS 60
           ME N TWS+V LPPG +SIGCKWVYKVKHN DGS+E YKARLVAKGYTQQE +D+IET S
Sbjct: 219 MESNNTWSVV-LPPGHHSIGCKWVYKVKHNPDGSIERYKARLVAKGYTQQEGLDYIETFS 278

Query: 61  PIAKLVVVKVLLTIV 76
            +AKLV VK+LLT+V
Sbjct: 279 LVAKLVNVKILLTLV 292

[Close]

BLAST of Moc11g13120 vs. ExPASy TrEMBL
Match: A5C102 (Integrase catalytic domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_001913 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 9.1e-23
Identity = 53/78 (67.95%), Postives = 65/78 (83.33%), Query Frame = 0


Query: 1   MEVNKTWSIVHLPPGQYSIGCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLS 60
           ME+NKTWS+V LP G++SIGCKW+YKVK  SDGS+E +KA +VAKGYTQQE +DF ET S
Sbjct: 455 MELNKTWSVVSLPKGKHSIGCKWIYKVKFKSDGSIERHKAPIVAKGYTQQEGLDFFETFS 514

Query: 61  PIAKLVVVKVLLTIVKMQ 79
           P+AKLV VKVLL +  +Q
Sbjct: 515 PVAKLVTVKVLLALATIQ 532

[Close]

BLAST of Moc11g13120 vs. ExPASy TrEMBL
Match: A0A438CKS1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_3919 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 9.1e-23
Identity = 53/78 (67.95%), Postives = 65/78 (83.33%), Query Frame = 0


Query: 1   MEVNKTWSIVHLPPGQYSIGCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLS 60
           ME+NKTWS+V LP G++SIGCKW+YKVK  SDGS+E +KA +VAKGYTQQE +DF ET S
Sbjct: 437 MELNKTWSVVSLPKGKHSIGCKWIYKVKFKSDGSIERHKAPIVAKGYTQQEGLDFFETFS 496

Query: 61  PIAKLVVVKVLLTIVKMQ 79
           P+AKLV VKVLL +  +Q
Sbjct: 497 PVAKLVTVKVLLALATIQ 514

[Close]

BLAST of Moc11g13120 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 108.6 bits (270), Expect = 3.6e-24
Identity = 49/74 (66.22%), Postives = 58/74 (78.38%), Query Frame = 0


Query: 1   MEVNKTWSIVHLPPGQYSIGCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLS 60
           ME   TW I  LPP +  IGCKWVYK+K+NSDG++E YKARLVAKGYTQQE +DFIET S
Sbjct: 109 METTHTWEICTLPPNKKPIGCKWVYKIKYNSDGTIERYKARLVAKGYTQQEGIDFIETFS 168

Query: 61  PIAKLVVVKVLLTI 75
           P+ KL  VK++L I
Sbjct: 169 PVCKLTSVKLILAI 182

[Close]

BLAST of Moc11g13120 vs. TAIR 10
Match: ATMG00820.1 (Reverse transcriptase (RNA-dependent DNA polymerase) )

HSP 1 Score: 74.7 bits (182), Expect = 5.8e-14
Identity = 40/95 (42.11%), Postives = 60/95 (63.16%), Query Frame = 0


Query: 4   NKTWSIVHLPPGQYSIGCKWVYKVKHNSDGSVEHYKARLVAKGYTQQEDVDFIETLSPIA 63
           NKTW +V  P  Q  +GCKWV+K K +SDG+++  KARLVAKG+ Q+E + F+ET SP+ 
Sbjct: 54  NKTWILVPPPVNQNILGCKWVFKTKLHSDGTLDRLKARLVAKGFHQEEGIYFVETYSPVV 113

Query: 64  KLVVVKVLLTIVKMQMICISNLIIIFFKK--NMGI 97
           +   ++ +L +   Q + +   I   FK   +MGI
Sbjct: 114 RTATIRTILNVA--QQLEVGQSINWMFKMHFSMGI 146

The following BLAST results are available for this feature:

BLAST of Moc11g13120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154919.1	3.8e-23	74.32	uncharacterized protein LOC111022065 [Momordica charantia]	[more]
XP_022156273.1	4.9e-23	74.32	uncharacterized protein LOC111023206 [Momordica charantia]	[more]
XP_022147774.1	6.5e-23	76.00	uncharacterized protein LOC111016631 isoform X1 [Momordica charantia] >XP_022147...	[more]
KAG7554761.1	1.1e-22	52.38	Retrotransposon gag domain [Arabidopsis suecica]	[more]
RVW99848.1	1.9e-22	67.95	Retrovirus-related Pol polyprotein from transposon RE1 [Vitis vinifera]	[more]

BLAST of Moc11g13120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94HW2	9.7e-14	47.22	Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O...	[more]
Q9ZT94	3.7e-13	47.22	Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O...	[more]
P92520	8.2e-13	42.11	Uncharacterized mitochondrial protein AtMg00820 OS=Arabidopsis thaliana OX=3702 ...	[more]
P10978	1.6e-11	39.19	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum...	[more]
P04146	1.7e-10	37.84	Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3	[more]

BLAST of Moc11g13120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DNP7	1.8e-23	74.32	uncharacterized protein LOC111022065 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1DQ66	2.4e-23	74.32	uncharacterized protein LOC111023206 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1D203	3.1e-23	76.00	uncharacterized protein LOC111016631 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A5C102	9.1e-23	67.95	Integrase catalytic domain-containing protein OS=Vitis vinifera OX=29760 GN=VITI...	[more]
A0A438CKS1	9.1e-23	67.95	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX...	[more]

BLAST of Moc11g13120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23160.1	3.6e-24	66.22	cysteine-rich RLK (RECEPTOR-like protein kinase) 8	[more]
ATMG00820.1	5.8e-14	42.11	Reverse transcriptase (RNA-dependent DNA polymerase)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013103	Reverse transcriptase, RNA-dependent DNA polymerase	PFAM	PF07727	RVT_2	coord: 4..83 e-value: 2.9E-19 score: 69.6
None	No IPR available	PANTHER	PTHR11439:SF302	REVERSE TRANSCRIPTASE, RNA-DEPENDENT DNA POLYMERASE-RELATED	coord: 13..75
None	No IPR available	PANTHER	PTHR11439	GAG-POL-RELATED RETROTRANSPOSON	coord: 13..75

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc11g13120.1	Moc11g13120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003676	nucleic acid binding