Moc11g12660 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc11g12660
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	DUF2431 domain-containing protein
Location	chr11: 9364142 .. 9365072 (-)
RNA-Seq Expression	Moc11g12660
Synteny	Moc11g12660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGACCTAAACAACAGTAGAGATCAGAAATGGATCAAGCATTACAGCAGCTCCCACACCATACTCCTCGTCGGAGAAGGCGACTTCTCCTTCTCGACGTGCTTAGCCACAACCTTCGGGTCTGCCGCTAACATGGTTGCCACTTCTCTGGACTCGAAAGGTACGTCTGTTTTTATTTTCTCTCTCGTACATTCAACTGTATATAAAACCCCACTAGCCATATCGAATAATTTTTCTGAATTTGCAATTGCAAGAGTCCCTACTGAGCAAGTACACTCACGTCGCCACAAACTTGAAAGAGTTGGAGGAGCTCGGCTGCACGGTTGCGCACGACGTGGACGCGACATCGATGAGCCAACACCCACTTCTCCGACACAAGTCATTCGACCGCGTCGTCTTCAACTTCCCCCATGCTGGATTTCATTTCAAAGAAAGTGACATTAGACAAATAGAGTGAGTATATTTCATTTCAAAGAAAGTGGATTGGATTGAGTGGTGGTTTATTCATCGTTAACAATAGTCTAGTTTAATTTCCTCTCTACTAGACTTCCTCTCTACTAGACTCCATCGACATCTAGTTCGAGGGTTTTTGAGGAACGCGAAGGAATTTTTGGGCGAAGAGGGTGAAATTCACATCACCCACAAGACTGCACATCCTTTCAGCAGATGGGAGATTGTGGAACTGGCTCGAGAGGAGGGGCTGCTTCTGAAAGAGGAACCAAATTTTTTTCTATGGGAATATCCAAATTATGAGAATAAGAGAGGCGATGGGTGGAACAGCGACGGCACTTTCCCAGTAGGAGCTTGCAGAACTTTCAAGTTTGCAATTACGGATGATGATCAATCGGACTCCTCGTTTGAGGCACTGATTCAATCCCTTTGTCTTACGGAAGACATTATAGATGACCACTCTCTAAATTATTTATAA

mRNA sequence

ATGGAGGACCTAAACAACAGTAGAGATCAGAAATGGATCAAGCATTACAGCAGCTCCCACACCATACTCCTCGTCGGAGAAGGCGACTTCTCCTTCTCGACGTGCTTAGCCACAACCTTCGGGTCTGCCGCTAACATGGTTGCCACTTCTCTGGACTCGAAAGAGTCCCTACTGAGCAAGTACACTCACGTCGCCACAAACTTGAAAGAGTTGGAGGAGCTCGGCTGCACGGTTGCGCACGACGTGGACGCGACATCGATGAGCCAACACCCACTTCTCCGACACAAGTCATTCGACCGCGTCGTCTTCAACTTCCCCCATGCTGGATTTCATTTCAAAGAAAGTGACATTAGACAAATAGAACTCCATCGACATCTAGTTCGAGGGTTTTTGAGGAACGCGAAGGAATTTTTGGGCGAAGAGGGTGAAATTCACATCACCCACAAGACTGCACATCCTTTCAGCAGATGGGAGATTGTGGAACTGGCTCGAGAGGAGGGGCTGCTTCTGAAAGAGGAACCAAATTTTTTTCTATGGGAATATCCAAATTATGAGAATAAGAGAGGCGATGGGTGGAACAGCGACGGCACTTTCCCAGTAGGAGCTTGCAGAACTTTCAAGTTTGCAATTACGGATGATGATCAATCGGACTCCTCGTTTGAGGCACTGATTCAATCCCTTTGTCTTACGGAAGACATTATAGATGACCACTCTCTAAATTATTTATAA

Coding sequence (CDS)

ATGGAGGACCTAAACAACAGTAGAGATCAGAAATGGATCAAGCATTACAGCAGCTCCCACACCATACTCCTCGTCGGAGAAGGCGACTTCTCCTTCTCGACGTGCTTAGCCACAACCTTCGGGTCTGCCGCTAACATGGTTGCCACTTCTCTGGACTCGAAAGAGTCCCTACTGAGCAAGTACACTCACGTCGCCACAAACTTGAAAGAGTTGGAGGAGCTCGGCTGCACGGTTGCGCACGACGTGGACGCGACATCGATGAGCCAACACCCACTTCTCCGACACAAGTCATTCGACCGCGTCGTCTTCAACTTCCCCCATGCTGGATTTCATTTCAAAGAAAGTGACATTAGACAAATAGAACTCCATCGACATCTAGTTCGAGGGTTTTTGAGGAACGCGAAGGAATTTTTGGGCGAAGAGGGTGAAATTCACATCACCCACAAGACTGCACATCCTTTCAGCAGATGGGAGATTGTGGAACTGGCTCGAGAGGAGGGGCTGCTTCTGAAAGAGGAACCAAATTTTTTTCTATGGGAATATCCAAATTATGAGAATAAGAGAGGCGATGGGTGGAACAGCGACGGCACTTTCCCAGTAGGAGCTTGCAGAACTTTCAAGTTTGCAATTACGGATGATGATCAATCGGACTCCTCGTTTGAGGCACTGATTCAATCCCTTTGTCTTACGGAAGACATTATAGATGACCACTCTCTAAATTATTTATAA

Protein sequence

MEDLNNSRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSKYTHVATNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQIELHRHLVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEYPNYENKRGDGWNSDGTFPVGACRTFKFAITDDDQSDSSFEALIQSLCLTEDIIDDHSLNYL

Homology

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BLAST of Moc11g12660 vs. NCBI nr
Match: XP_022139691.1 (uncharacterized protein At4g26485-like [Momordica charantia])

HSP 1 Score: 499.2 bits (1284), Expect = 2.0e-137
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 0


Query: 1   MEDLNNSRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSK 60
           MEDLNNSRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSK
Sbjct: 1   MEDLNNSRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSK 60

Query: 61  YTHVATNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQI 120
           YTHVATNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQI
Sbjct: 61  YTHVATNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQI 120

Query: 121 ELHRHLVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWE 180
           ELHRHLVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWE
Sbjct: 121 ELHRHLVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWE 180

Query: 181 YPNYENKRGDGWNSDGTFPVGACRTFKFAITDDDQSDSSFEALIQSLCLTEDIIDDHSLN 240
           YPNYENKRGDGWNSDGTFPVGACRTFKFAITDDDQSDSSFEALIQSLCLTEDIIDDHSLN
Sbjct: 181 YPNYENKRGDGWNSDGTFPVGACRTFKFAITDDDQSDSSFEALIQSLCLTEDIIDDHSLN 240

Query: 241 YL 243
           YL
Sbjct: 241 YL 242

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BLAST of Moc11g12660 vs. NCBI nr
Match: TYK23208.1 (uncharacterized protein E5676_scaffold142G002400 [Cucumis melo var. makuwa])

HSP 1 Score: 302.8 bits (774), Expect = 2.7e-78
Identity = 145/203 (71.43%), Postives = 164/203 (80.79%), Query Frame = 0


Query: 6   NSRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSKYTHVA 65
           +S  +K IKHYSSSH ILLVGEGDFSFS CLAT F SA+N+VATSLDS + L+ KYTH  
Sbjct: 2   DSNTEKSIKHYSSSHKILLVGEGDFSFSACLATAFASASNIVATSLDSPDELVMKYTHAN 61

Query: 66  TNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQIELHRH 125
            NLK LEELG  V H VDAT+MSQHPLL    FDR+VFNFPHAGF + E +  QIELHR 
Sbjct: 62  RNLKILEELGGRVLHKVDATTMSQHPLLYDMLFDRIVFNFPHAGFLYGECNTAQIELHRD 121

Query: 126 LVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEYPNYE 185
           LVRGFLRNAK  + ++GEIHITHKT+HPFS+WEIV+LA  EGL LKE   F+ W+YPNYE
Sbjct: 122 LVRGFLRNAKRMISKDGEIHITHKTSHPFSKWEIVKLASGEGLALKESAEFYAWQYPNYE 181

Query: 186 NKRGDGWNSDGTFPVGACRTFKF 209
           NKRGDGWNS+GTFPVGAC TFKF
Sbjct: 182 NKRGDGWNSNGTFPVGACSTFKF 204

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BLAST of Moc11g12660 vs. NCBI nr
Match: XP_008448100.1 (PREDICTED: uncharacterized protein At4g26485-like [Cucumis melo])

HSP 1 Score: 302.0 bits (772), Expect = 4.7e-78
Identity = 145/203 (71.43%), Postives = 164/203 (80.79%), Query Frame = 0


Query: 6   NSRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSKYTHVA 65
           +S  +K IKHYSSSH ILLVGEGDFSFS CLAT F SA+N+VATSLDS + L+ KYTH  
Sbjct: 2   DSDTEKSIKHYSSSHKILLVGEGDFSFSACLATAFASASNIVATSLDSPDELVMKYTHAN 61

Query: 66  TNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQIELHRH 125
            NLK LEELG  V H VDAT+MSQHPLL    FDR+VFNFPHAGF + E +  QIELHR 
Sbjct: 62  RNLKILEELGGRVLHKVDATTMSQHPLLYDMLFDRIVFNFPHAGFLYGECNTAQIELHRD 121

Query: 126 LVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEYPNYE 185
           LVRGFLRNAK  + ++GEIHITHKT+HPFS+WEIV+LA  EGL LKE   F+ W+YPNYE
Sbjct: 122 LVRGFLRNAKRMISKDGEIHITHKTSHPFSKWEIVKLASGEGLALKESAEFYAWQYPNYE 181

Query: 186 NKRGDGWNSDGTFPVGACRTFKF 209
           NKRGDGWNS+GTFPVGAC TFKF
Sbjct: 182 NKRGDGWNSNGTFPVGACSTFKF 204

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BLAST of Moc11g12660 vs. NCBI nr
Match: XP_011659737.1 (uncharacterized protein At4g26485 [Cucumis sativus] >KGN44112.1 hypothetical protein Csa_016883 [Cucumis sativus])

HSP 1 Score: 293.5 bits (750), Expect = 1.7e-75
Identity = 144/208 (69.23%), Postives = 165/208 (79.33%), Query Frame = 0


Query: 3   DLNNSRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSKYT 62
           D+NN+  +K I HYSSSH ILLVGEGDFSFS CLAT F SA+N++ATSLDS++ L+ KY 
Sbjct: 2   DINNT--EKSIMHYSSSHNILLVGEGDFSFSACLATAFASASNIIATSLDSRDELVMKYA 61

Query: 63  HVATNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQIEL 122
             A N+K LEELG TV H+VDAT+MSQHPLL    FDR+VFNFPHAGF +KES+  QIEL
Sbjct: 62  RAARNVKILEELGGTVLHEVDATTMSQHPLLHDMLFDRIVFNFPHAGFVYKESNTAQIEL 121

Query: 123 HRHLVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEYP 182
           HR+LVR FLRNAK  +  EGEIHITHKT+HPFS+W IV LA +EGL LKE   F  W+YP
Sbjct: 122 HRNLVRVFLRNAKSMIDVEGEIHITHKTSHPFSKWGIVNLACDEGLCLKESEIFHAWQYP 181

Query: 183 NYENKRGDGWNSDGTFPVGACRTFKFAI 211
           NYENKRG G N DGTFPVGAC TFKF I
Sbjct: 182 NYENKRGHGQNPDGTFPVGACSTFKFFI 207

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BLAST of Moc11g12660 vs. NCBI nr
Match: XP_022139692.1 (uncharacterized protein At4g26485-like [Momordica charantia])

HSP 1 Score: 288.1 bits (736), Expect = 7.0e-74
Identity = 147/209 (70.33%), Postives = 161/209 (77.03%), Query Frame = 0


Query: 1   MEDLNNSRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSK 60
           M DL N++DQKWIKHYSSSHTILL GEGDFSFSTCLA TFGSA NMVATSLDS+ SL SK
Sbjct: 1   MGDL-NTKDQKWIKHYSSSHTILLFGEGDFSFSTCLAKTFGSAVNMVATSLDSEVSLWSK 60

Query: 61  YTHVATNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQI 120
           Y  V TNLK LE+LGCTV HDVDAT+MSQHPL   +         P     F      Q 
Sbjct: 61  YIRVHTNLKVLEDLGCTVVHDVDATTMSQHPLRPRR------LQLPPCRISF------QR 120

Query: 121 ELHRHLVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWE 180
           +LHR LVRGF RNAK+ LGE GEIHITHKT +PFS W+IVELA++EGLLLKE   F LW+
Sbjct: 121 KLHRALVRGFFRNAKKLLGENGEIHITHKTTYPFSEWKIVELAQQEGLLLKERAYFLLWD 180

Query: 181 YPNYENKRGDGWNSDGTFPVGACRTFKFA 210
           YPNY NKRGDGWNSDGTFPVGAC TFKF+
Sbjct: 181 YPNYGNKRGDGWNSDGTFPVGACATFKFS 196

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BLAST of Moc11g12660 vs. ExPASy Swiss-Prot
Match: P0C8L4 (Uncharacterized protein At4g26485 OS=Arabidopsis thaliana OX=3702 GN=At4g26485 PE=4 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 2.0e-55
Identity = 111/202 (54.95%), Postives = 136/202 (67.33%), Query Frame = 0


Query: 10  QKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSKYTHVATNLK 69
           +KWI+HYSS+H ILLVGEG+FSFS CLA+ FGSA N+ ATSLDS++ L  KY     N+ 
Sbjct: 5   EKWIRHYSSTHKILLVGEGNFSFSLCLASAFGSAMNITATSLDSEDELSIKYMDAVDNIN 64

Query: 70  ELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHF--KESDIRQIELHRHLV 129
            L+  GC + H+VD  +MS    L  + +DR+VFNFPHAG  F  +E   R IE H+ LV
Sbjct: 65  ILKRYGCDIQHEVDVHTMSFDNSLSLQRYDRIVFNFPHAGSRFFGRELSSRAIESHKELV 124

Query: 130 RGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEYPNYENK 189
           RGFL NAKE L E+GEIHITHKT +PFS W I +L + EGL L ++  F L  YP Y  K
Sbjct: 125 RGFLENAKEMLEEDGEIHITHKTTYPFSDWGIKKLGKGEGLKLLKKSKFELSHYPGYITK 184

Query: 190 RGDGW-NSDGTFPVGACRTFKF 209
           RG G   SD  FPVG C T+ F
Sbjct: 185 RGSGGRRSDDYFPVGECSTYMF 206

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BLAST of Moc11g12660 vs. ExPASy Swiss-Prot
Match: F4I1X0 (Heavy metal-associated isoprenylated plant protein 41 OS=Arabidopsis thaliana OX=3702 GN=HIPP41 PE=3 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 3.4e-55
Identity = 110/211 (52.13%), Postives = 136/211 (64.45%), Query Frame = 0


Query: 1   MEDLNNSRD---QKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESL 60
           +  LN   D   + W+KHYSS+H ILLVGEGDFSFS  LAT FGSA+N+ A+SLDS + +
Sbjct: 6   LSQLNRDSDGDEEVWVKHYSSNHQILLVGEGDFSFSHSLATLFGSASNICASSLDSYDVV 65

Query: 61  LSKYTHVATNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDI 120
           + KY    +NLK L+ LG  + H VDAT++  HP LR++ FDRV+FNFPHAGFH +ESD 
Sbjct: 66  VRKYKKARSNLKTLKRLGALLLHGVDATTLHFHPDLRYRRFDRVIFNFPHAGFHGRESDS 125

Query: 121 RQIELHRHLVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFF 180
             I  HR LV GF   A   L   GE+H++HK   PFS W + ELA    L+L +   F 
Sbjct: 126 SLIRKHRELVFGFFNGASRLLRANGEVHVSHKNKAPFSEWNLEELASRCFLVLIQRVAFE 185

Query: 181 LWEYPNYENKRGDGWNSDGTFPVGACRTFKF 209
              YP YENKRGDG   D  F +G C TFKF
Sbjct: 186 KNNYPGYENKRGDGRRCDQPFLLGECSTFKF 216

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BLAST of Moc11g12660 vs. ExPASy Swiss-Prot
Match: O94480 (25S rRNA (uridine-N(3))-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bmt5 PE=3 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.3e-22
Identity = 63/189 (33.33%), Postives = 100/189 (52.91%), Query Frame = 0


Query: 5   NNSRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMV-ATSLDSKESLLSKYTH 64
           N    ++++  +  ++  LL+GEG+FSF+  L     S+   V ATS DSKE L  KY  
Sbjct: 43  NKGPTERYVLPFEKNNRFLLLGEGNFSFAFSLLLHHVSSEGFVLATSYDSKEDLKQKYPD 102

Query: 65  VATNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQIELH 124
            A  + ++E  G  V H++DAT +  H  L+ + FD + +NFPH+G   K+ D R I  +
Sbjct: 103 AAEYISKIEINGGKVMHEIDATKLHLHKKLKTQKFDTIFWNFPHSGKGIKDQD-RNILDN 162

Query: 125 RHLVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEYPN 184
           + ++  F + +K  L E+G I IT     P++ W +  LA++ G        F    YP 
Sbjct: 163 QKMLLAFFKASKFLLSEKGVIVITLAETKPYTLWNLKGLAKDAGYTSLMTEKFDSSFYPE 222

Query: 185 YENKRGDGW 193
           Y ++R  GW
Sbjct: 223 YSHRRTIGW 230

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BLAST of Moc11g12660 vs. ExPASy Swiss-Prot
Match: Q3UY23 (Ferredoxin-fold anticodon-binding domain-containing protein 1 homolog OS=Mus musculus OX=10090 GN=Fdxacb1 PE=2 SV=2)

HSP 1 Score: 71.6 bits (174), Expect = 1.3e-11
Identity = 49/175 (28.00%), Postives = 80/175 (45.71%), Query Frame = 0


Query: 22  ILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSKYTHVATNLKELEELGCTVAHD 81
           +LLVGEG+FSF+  L      + ++ AT    + +L         NLK L E G  V   
Sbjct: 6   LLLVGEGNFSFAASLIDGLDPSVSVTATGFQHRAALEGDPV-ALENLKRLRERGVEVRFG 65

Query: 82  VDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQIELHRHLVRGFLRNAKEFLGEE 141
           VD T +S       + FDR+ FNFPH G   +++ + +   +R L+  F ++  + L + 
Sbjct: 66  VDCTQLSHALPADDRDFDRIYFNFPHCG---RKAGVAK---NRELLAKFFQSCADVLAKA 125

Query: 142 GEIHIT-----------HKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEYPNYE 186
           GE+H+T                  + W++V +A   G +L +   F     P Y+
Sbjct: 126 GEVHVTLCRGQGGTPADKPQREWHNSWQVVAMAALGGFILSDVCPFSCEAVPGYK 173

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BLAST of Moc11g12660 vs. ExPASy Swiss-Prot
Match: Q9BRP7 (Ferredoxin-fold anticodon-binding domain-containing protein 1 OS=Homo sapiens OX=9606 GN=FDXACB1 PE=1 SV=3)

HSP 1 Score: 70.5 bits (171), Expect = 3.0e-11
Identity = 47/162 (29.01%), Postives = 81/162 (50.00%), Query Frame = 0


Query: 22  ILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSKYTHVATNLKELEELGCTVAHD 81
           +LLVGEG+FSF+  L+ T   +  + AT L  + + L++      NL+ L E G  V   
Sbjct: 6   LLLVGEGNFSFAAALSETLDQSTQLTATCL-QRPAELARDPLAWENLQCLRERGIDVRFG 65

Query: 82  VDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQIELHRHLVRGFLRNAKEFLGEE 141
           VD T ++    L  + FD++ F FPH G   +++ + +   +R L+  F ++  + L EE
Sbjct: 66  VDCTQLADVFELHEREFDQIYFIFPHCG---RKAGVAK---NRELLAKFFQSCADVLAEE 125

Query: 142 GEIHIT-----------HKTAHPFSRWEIVELAREEGLLLKE 173
           GE+H+                   + W++V +A   GL+L +
Sbjct: 126 GEVHVALCRGQGGTPADKPQREWHNSWQVVAMAALGGLILSD 160

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BLAST of Moc11g12660 vs. ExPASy TrEMBL
Match: A0A6J1CDH1 (uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC111010537 PE=4 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 9.7e-138
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 0


Query: 1   MEDLNNSRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSK 60
           MEDLNNSRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSK
Sbjct: 1   MEDLNNSRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSK 60

Query: 61  YTHVATNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQI 120
           YTHVATNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQI
Sbjct: 61  YTHVATNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQI 120

Query: 121 ELHRHLVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWE 180
           ELHRHLVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWE
Sbjct: 121 ELHRHLVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWE 180

Query: 181 YPNYENKRGDGWNSDGTFPVGACRTFKFAITDDDQSDSSFEALIQSLCLTEDIIDDHSLN 240
           YPNYENKRGDGWNSDGTFPVGACRTFKFAITDDDQSDSSFEALIQSLCLTEDIIDDHSLN
Sbjct: 181 YPNYENKRGDGWNSDGTFPVGACRTFKFAITDDDQSDSSFEALIQSLCLTEDIIDDHSLN 240

Query: 241 YL 243
           YL
Sbjct: 241 YL 242

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BLAST of Moc11g12660 vs. ExPASy TrEMBL
Match: A0A5D3DIB6 (DUF2431 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G002400 PE=4 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 1.3e-78
Identity = 145/203 (71.43%), Postives = 164/203 (80.79%), Query Frame = 0


Query: 6   NSRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSKYTHVA 65
           +S  +K IKHYSSSH ILLVGEGDFSFS CLAT F SA+N+VATSLDS + L+ KYTH  
Sbjct: 2   DSNTEKSIKHYSSSHKILLVGEGDFSFSACLATAFASASNIVATSLDSPDELVMKYTHAN 61

Query: 66  TNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQIELHRH 125
            NLK LEELG  V H VDAT+MSQHPLL    FDR+VFNFPHAGF + E +  QIELHR 
Sbjct: 62  RNLKILEELGGRVLHKVDATTMSQHPLLYDMLFDRIVFNFPHAGFLYGECNTAQIELHRD 121

Query: 126 LVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEYPNYE 185
           LVRGFLRNAK  + ++GEIHITHKT+HPFS+WEIV+LA  EGL LKE   F+ W+YPNYE
Sbjct: 122 LVRGFLRNAKRMISKDGEIHITHKTSHPFSKWEIVKLASGEGLALKESAEFYAWQYPNYE 181

Query: 186 NKRGDGWNSDGTFPVGACRTFKF 209
           NKRGDGWNS+GTFPVGAC TFKF
Sbjct: 182 NKRGDGWNSNGTFPVGACSTFKF 204

[Close]

BLAST of Moc11g12660 vs. ExPASy TrEMBL
Match: A0A1S3BIC4 (uncharacterized protein At4g26485-like OS=Cucumis melo OX=3656 GN=LOC103490386 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 2.3e-78
Identity = 145/203 (71.43%), Postives = 164/203 (80.79%), Query Frame = 0


Query: 6   NSRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSKYTHVA 65
           +S  +K IKHYSSSH ILLVGEGDFSFS CLAT F SA+N+VATSLDS + L+ KYTH  
Sbjct: 2   DSDTEKSIKHYSSSHKILLVGEGDFSFSACLATAFASASNIVATSLDSPDELVMKYTHAN 61

Query: 66  TNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQIELHRH 125
            NLK LEELG  V H VDAT+MSQHPLL    FDR+VFNFPHAGF + E +  QIELHR 
Sbjct: 62  RNLKILEELGGRVLHKVDATTMSQHPLLYDMLFDRIVFNFPHAGFLYGECNTAQIELHRD 121

Query: 126 LVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEYPNYE 185
           LVRGFLRNAK  + ++GEIHITHKT+HPFS+WEIV+LA  EGL LKE   F+ W+YPNYE
Sbjct: 122 LVRGFLRNAKRMISKDGEIHITHKTSHPFSKWEIVKLASGEGLALKESAEFYAWQYPNYE 181

Query: 186 NKRGDGWNSDGTFPVGACRTFKF 209
           NKRGDGWNS+GTFPVGAC TFKF
Sbjct: 182 NKRGDGWNSNGTFPVGACSTFKF 204

[Close]

BLAST of Moc11g12660 vs. ExPASy TrEMBL
Match: A0A0A0K734 (DUF2431 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G193720 PE=4 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 8.0e-76
Identity = 144/208 (69.23%), Postives = 165/208 (79.33%), Query Frame = 0


Query: 3   DLNNSRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSKYT 62
           D+NN+  +K I HYSSSH ILLVGEGDFSFS CLAT F SA+N++ATSLDS++ L+ KY 
Sbjct: 2   DINNT--EKSIMHYSSSHNILLVGEGDFSFSACLATAFASASNIIATSLDSRDELVMKYA 61

Query: 63  HVATNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQIEL 122
             A N+K LEELG TV H+VDAT+MSQHPLL    FDR+VFNFPHAGF +KES+  QIEL
Sbjct: 62  RAARNVKILEELGGTVLHEVDATTMSQHPLLHDMLFDRIVFNFPHAGFVYKESNTAQIEL 121

Query: 123 HRHLVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEYP 182
           HR+LVR FLRNAK  +  EGEIHITHKT+HPFS+W IV LA +EGL LKE   F  W+YP
Sbjct: 122 HRNLVRVFLRNAKSMIDVEGEIHITHKTSHPFSKWGIVNLACDEGLCLKESEIFHAWQYP 181

Query: 183 NYENKRGDGWNSDGTFPVGACRTFKFAI 211
           NYENKRG G N DGTFPVGAC TFKF I
Sbjct: 182 NYENKRGHGQNPDGTFPVGACSTFKFFI 207

[Close]

BLAST of Moc11g12660 vs. ExPASy TrEMBL
Match: A0A6J1CEN8 (uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC111010538 PE=4 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 3.4e-74
Identity = 147/209 (70.33%), Postives = 161/209 (77.03%), Query Frame = 0


Query: 1   MEDLNNSRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSK 60
           M DL N++DQKWIKHYSSSHTILL GEGDFSFSTCLA TFGSA NMVATSLDS+ SL SK
Sbjct: 1   MGDL-NTKDQKWIKHYSSSHTILLFGEGDFSFSTCLAKTFGSAVNMVATSLDSEVSLWSK 60

Query: 61  YTHVATNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDIRQI 120
           Y  V TNLK LE+LGCTV HDVDAT+MSQHPL   +         P     F      Q 
Sbjct: 61  YIRVHTNLKVLEDLGCTVVHDVDATTMSQHPLRPRR------LQLPPCRISF------QR 120

Query: 121 ELHRHLVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWE 180
           +LHR LVRGF RNAK+ LGE GEIHITHKT +PFS W+IVELA++EGLLLKE   F LW+
Sbjct: 121 KLHRALVRGFFRNAKKLLGENGEIHITHKTTYPFSEWKIVELAQQEGLLLKERAYFLLWD 180

Query: 181 YPNYENKRGDGWNSDGTFPVGACRTFKFA 210
           YPNY NKRGDGWNSDGTFPVGAC TFKF+
Sbjct: 181 YPNYGNKRGDGWNSDGTFPVGACATFKFS 196

[Close]

BLAST of Moc11g12660 vs. TAIR 10
Match: AT1G55790.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 216.5 bits (550), Expect = 2.4e-56
Identity = 110/211 (52.13%), Postives = 136/211 (64.45%), Query Frame = 0


Query: 1   MEDLNNSRD---QKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESL 60
           +  LN   D   + W+KHYSS+H ILLVGEGDFSFS  LAT FGSA+N+ A+SLDS + +
Sbjct: 6   LSQLNRDSDGDEEVWVKHYSSNHQILLVGEGDFSFSHSLATLFGSASNICASSLDSYDVV 65

Query: 61  LSKYTHVATNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHFKESDI 120
           + KY    +NLK L+ LG  + H VDAT++  HP LR++ FDRV+FNFPHAGFH +ESD 
Sbjct: 66  VRKYKKARSNLKTLKRLGALLLHGVDATTLHFHPDLRYRRFDRVIFNFPHAGFHGRESDS 125

Query: 121 RQIELHRHLVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFF 180
             I  HR LV GF   A   L   GE+H++HK   PFS W + ELA    L+L +   F 
Sbjct: 126 SLIRKHRELVFGFFNGASRLLRANGEVHVSHKNKAPFSEWNLEELASRCFLVLIQRVAFE 185

Query: 181 LWEYPNYENKRGDGWNSDGTFPVGACRTFKF 209
              YP YENKRGDG   D  F +G C TFKF
Sbjct: 186 KNNYPGYENKRGDGRRCDQPFLLGECSTFKF 216

[Close]

BLAST of Moc11g12660 vs. TAIR 10
Match: AT5G56060.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 181.8 bits (460), Expect = 6.6e-46
Identity = 99/188 (52.66%), Postives = 123/188 (65.43%), Query Frame = 0


Query: 13  IKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSKYTHVATNLKELE 72
           ++ YS+   ILLVGEGDFSFS  LA  FGSA N+ ATSLD++E L  KYT    N++ LE
Sbjct: 9   LQQYSNKQKILLVGEGDFSFSLSLARVFGSATNITATSLDTREELGIKYTDGKANVEGLE 68

Query: 73  ELGCTVAHDVDATSMSQHPLLRHKSFDRVVFNFPHAGFHF-KESDIRQIELHRHLVRGFL 132
             GCTV H V+  SMS     R   +DR++FNFPH+G  F  E DI  I LH+ LVRGFL
Sbjct: 69  LFGCTVVHGVNVHSMSSD--YRLGRYDRIIFNFPHSGLGFGSEHDIFFIMLHQGLVRGFL 128

Query: 133 RNAKEFL-GEEGEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEYPNYENKRGD 192
            +A++ L  E+GEIH+THKT  PF+RW I  LA E+GL L  E  F  W +P Y NK+G 
Sbjct: 129 ESARKMLKDEDGEIHVTHKTTDPFNRWGIETLAGEKGLRLIGEIEFHKWAFPGYSNKKGG 188

Query: 193 GWNSDGTF 199
           G N + TF
Sbjct: 189 GSNCNSTF 194

[Close]

BLAST of Moc11g12660 vs. TAIR 10
Match: AT5G56075.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 167.5 bits (423), Expect = 1.3e-41
Identity = 92/207 (44.44%), Postives = 125/207 (60.39%), Query Frame = 0


Query: 6   NSRDQKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDSKESLLSKYTHVA 65
           N ++ K ++HY++   ILLVGEGDFSFS  LA  FGSA+N+ ATSLD++  L  K+ +  
Sbjct: 60  NVQETKRLRHYTNKQKILLVGEGDFSFSLSLARAFGSASNLTATSLDTQGELEQKFKNGK 119

Query: 66  TNLKELEELGCTVAHDVDATSMSQHPLLRHKS-FDRVVFNFPHAGFHFKESDIRQIELHR 125
            N++ELE LGC+V + V+  SM   P +   + +DRV+FNFP                  
Sbjct: 120 ANVEELERLGCSVVYGVNVHSMITKPSVGGSAIYDRVIFNFP----------------TH 179

Query: 126 HLVRGFLRNAKEFLGEE---GEIHITHKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEY 185
            LVRGF+++A+  + +E   GEIH+ HKT +PFS W++  L  +EGL L  E  F L  Y
Sbjct: 180 ELVRGFMKSARVLVKDEDKGGEIHVIHKTEYPFSEWKLKTLGEKEGLDLIREVEFCLSHY 239

Query: 186 PNYENKRGDGWNSDGTFPVGACRTFKF 209
           P Y NKRG G  SD +FPVG   TF F
Sbjct: 240 PGYFNKRGSGGYSDSSFPVGKSSTFMF 250

[Close]

BLAST of Moc11g12660 vs. TAIR 10
Match: AT4G26485.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 162.2 bits (409), Expect = 5.4e-40
Identity = 85/167 (50.90%), Postives = 105/167 (62.87%), Query Frame = 0


Query: 45  NMVATSLDSKESLLSKYTHVATNLKELEELGCTVAHDVDATSMSQHPLLRHKSFDRVVFN 104
           N+ ATSLDS++ L  KY     N+  L+  GC + H+VD  +MS    L  + +DR+VFN
Sbjct: 2   NITATSLDSEDELSIKYMDAVDNINILKRYGCDIQHEVDVHTMSFDNSLSLQRYDRIVFN 61

Query: 105 FPHAGFHF--KESDIRQIELHRHLVRGFLRNAKEFLGEEGEIHITHKTAHPFSRWEIVEL 164
           FPHAG  F  +E   R IE H+ LVRGFL NAKE L E+GEIHITHKT +PFS W I +L
Sbjct: 62  FPHAGSRFFGRELSSRAIESHKELVRGFLENAKEMLEEDGEIHITHKTTYPFSDWGIKKL 121

Query: 165 AREEGLLLKEEPNFFLWEYPNYENKRGDGW-NSDGTFPVGACRTFKF 209
            + EGL L ++  F L  YP Y  KRG G   SD  FPVG C T+ F
Sbjct: 122 GKGEGLKLLKKSKFELSHYPGYITKRGSGGRRSDDYFPVGECSTYMF 168

[Close]

BLAST of Moc11g12660 vs. TAIR 10
Match: AT1G55800.1 (Domain of unknown function (DUF2431) )

HSP 1 Score: 159.1 bits (401), Expect = 4.6e-39
Identity = 97/242 (40.08%), Postives = 123/242 (50.83%), Query Frame = 0


Query: 1   MEDLNNSRD---QKWIKHYSSSHTILLVGEGDFSFSTCLATTFGSAANMVATSLDS---- 60
           + +LN  RD   + W+KHYSS H ILLVGEGDFSFS  LAT FGSA+N+ A+SLDS    
Sbjct: 4   LSELNRDRDGDEEVWVKHYSSKHQILLVGEGDFSFSCSLATCFGSASNIYASSLDSYDYK 63

Query: 61  -----------------------KESLLSKYTHVATNLKELEELGCTVAHDVDATSMSQH 120
                                   + ++ KY +  +NL+ L+ LG  + H VDAT++  H
Sbjct: 64  PVNKGCSFKLDFLSCCMSFMVIKPDDVVRKYKNARSNLETLKRLGAFLLHGVDATTLHFH 123

Query: 121 PLLRHKSFDRVVFNFPHAGFHFKESDIRQIE----LHRHLVRGFLRNAKEFLGEEGEIHI 180
           P LR++ FDRV+FNFPH GFH KESD  QI+      R+L + FL  A   L  +GE+  
Sbjct: 124 PDLRYRRFDRVIFNFPHTGFHRKESDPCQIQPAAATLRNLFKDFLHGASHMLRADGELEA 183

Query: 181 THKTAHPFSRWEIVELAREEGLLLKEEPNFFLWEYPNYENKRGDGWNSDGTFPVGACRTF 209
             K                               YP YENKRGDG   D  F +G C TF
Sbjct: 184 FEKR-----------------------------NYPGYENKRGDGSRCDQPFLLGECSTF 216

The following BLAST results are available for this feature:

BLAST of Moc11g12660 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139691.1	2.0e-137	100.00	uncharacterized protein At4g26485-like [Momordica charantia]	[more]
TYK23208.1	2.7e-78	71.43	uncharacterized protein E5676_scaffold142G002400 [Cucumis melo var. makuwa]	[more]
XP_008448100.1	4.7e-78	71.43	PREDICTED: uncharacterized protein At4g26485-like [Cucumis melo]	[more]
XP_011659737.1	1.7e-75	69.23	uncharacterized protein At4g26485 [Cucumis sativus] >KGN44112.1 hypothetical pro...	[more]
XP_022139692.1	7.0e-74	70.33	uncharacterized protein At4g26485-like [Momordica charantia]	[more]

BLAST of Moc11g12660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0C8L4	2.0e-55	54.95	Uncharacterized protein At4g26485 OS=Arabidopsis thaliana OX=3702 GN=At4g26485 P...	[more]
F4I1X0	3.4e-55	52.13	Heavy metal-associated isoprenylated plant protein 41 OS=Arabidopsis thaliana OX...	[more]
O94480	1.3e-22	33.33	25S rRNA (uridine-N(3))-methyltransferase OS=Schizosaccharomyces pombe (strain 9...	[more]
Q3UY23	1.3e-11	28.00	Ferredoxin-fold anticodon-binding domain-containing protein 1 homolog OS=Mus mus...	[more]
Q9BRP7	3.0e-11	29.01	Ferredoxin-fold anticodon-binding domain-containing protein 1 OS=Homo sapiens OX...	[more]

BLAST of Moc11g12660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CDH1	9.7e-138	100.00	uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A5D3DIB6	1.3e-78	71.43	DUF2431 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3BIC4	2.3e-78	71.43	uncharacterized protein At4g26485-like OS=Cucumis melo OX=3656 GN=LOC103490386 P...	[more]
A0A0A0K734	8.0e-76	69.23	DUF2431 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G193720 PE=...	[more]
A0A6J1CEN8	3.4e-74	70.33	uncharacterized protein At4g26485-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of Moc11g12660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55790.1	2.4e-56	52.13	Domain of unknown function (DUF2431)	[more]
AT5G56060.1	6.6e-46	52.66	Domain of unknown function (DUF2431)	[more]
AT5G56075.1	1.3e-41	44.44	Domain of unknown function (DUF2431)	[more]
AT4G26485.1	5.4e-40	50.90	Domain of unknown function (DUF2431)	[more]
AT1G55800.1	4.6e-39	40.08	Domain of unknown function (DUF2431)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019446	Domain of unknown function DUF2431	PFAM	PF10354	DUF2431	coord: 23..188 e-value: 1.0E-50 score: 172.3
None	No IPR available	PANTHER	PTHR11538:SF66	DUF2431 DOMAIN PROTEIN	coord: 9..209
None	No IPR available	PANTHER	PTHR11538	PHENYLALANYL-TRNA SYNTHETASE	coord: 9..209
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 70..171

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc11g12660.1	Moc11g12660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070475	rRNA base methylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0070042	rRNA (uridine-N3-)-methyltransferase activity