Moc11g10090 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc11g10090
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	E3 SUMO-protein ligase MMS21
Location	chr11: 7255493 .. 7258467 (-)
RNA-Seq Expression	Moc11g10090
Synteny	Moc11g10090

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCGACTTCTGATACCCGTTCAAGTGCCGTTTCGGGCAGAATCAAATCCGCTGCCACTATGATGCACTCCGAGAATCAGTCCCTGCTTGCTGTAAATTGCTTAATACTACTCGCTATTTATATGATTACTTCTAATTTGTTGCCGTTTCGGTTCTAGTTTTTTTTTTTTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTCTGATTGATAGAGAAATTTGGTTTGGGAAATGCGGCGCTGAGAAGAAAAGGAAGTTGTTGGTGCTAAATTAATGTTGTTTTCAGCTTTAAGATCTCGATTTTTTTGTATAGAGAGTCGGTTATAATGCTGTATTCCTTTAATTCTGGCGCTGTTTCTCTGGATTTCTGAAACGTCGGGGTCTTGGGCGCGAGCTGAGAAGCTACAGGAAGATATCGTATGTATTTTCTCATAGAAAATCCCACCCAATAACCTGTTTTGTTCAGTTCGTGGTCTGATAAGACAATTAAGAGATTCGTCAATTAAATTTAAAGGGAAGATAAGAGAACGTTCTTCTGATGTACTCCTCAGTCATCTCGCACTTGAGTGGGTTAATCTACTACAATCCTGTCTTGTTCAAAAGAACGATTTATTTGGATATTAGGCTAATGATGATGGGTTATCTGTTGATTTTTGTGATGTGATTCAACTTTTCTATTTTAATTTCAGGAAATACGCAAGTTGTTGATTATGATGAAGGAAATTGGGATGGATTTGGAGAGGGACAATCAGTCGACGATGGTATTATGGATAAAGAACCCTTGTTTCAATTTACTTTGGAAGAGTGTTTTCAGGATAAGATCTTGTAATAAAATGTTAGAAGCAAGAACACAAATTTCAAAGATTTTTTTTCTAGGACTCGCATCATAAGGCTTTATTTTAACATCTTTGTTGCCTTTAAAAAAAAAATTAATTGAAGAAACGACGTGGGTAAAGGAAAACAAGAAGCATGTGCAGTGTACTGTGTACAATCTGCTTTAGAACTTGCAATATAAACCAGCCACTTTGTCAAGCCGTCAAAATCAAATATTTGAAATGTTTATTTCTGTTCGGTCATTTTGCTTGTTTAGTAACCGACATATTGCTTTTTGATGGACTTTCTGTGGATGCCTCGATTTGAAGAAGATTTATTTCAACGGATACCTTGCTTAAAGATTATGGTCTTGGTTTCCCTAGGTGTACGGTTAATTAATTAGAATGCAGCACATACTTTTTGGTGGATTTTCTTCTTCTGGCTATTAAATCCGGTCTAAAGATTATTGAGAATCTTTGTTGTTTGGAAGTCTTAACCAATTATTTACTTGGTATTTGAAGGTCAAGAAGCTTGAAAATGCTGTTGTTGAGCTGTTGAGTACTTACGAAAACTGTGACAACTTTTCATCTGCGATTCAGTCGGTTGGAAATATATATGAACCAAGAGAAGAGGTATTTAAATCTCTTTCCATGTTGAATTTCCCACGATATATTCGACATTCAGTTTGTTAATTTTCATAGTGCTGGTTCCTTCGTTTCCATACCTAATGATAGTTTATGTTGATCCTGTAGTTAACAGATTTTGAGAAACTACTTGACGATGAAGTTGCAAAAGTCAGTGAAAATTCATCTTCAAATTTGCAGAACCATTCAATAATTCGGAAGTTTAGAGAAGCTATTTGGGTATGCATCTTCATCTTATTTTCTATGCTCCGGGACAGGCAATTGGCACCTTGAAAGACAGTAGATTAGAAAGCCAGTGGAAAGTATTTCCATTTTATTAACTGATTATAGGGATTGCGATACCTCTTTGGCACTGATTACGCATGGTAGCAACAAAAAAGTTGTCAAATCTGTGCAAATAATCATCCTAAAACTGTGAAAACGCAACAGAAAGTACATGATAAGTAAACAAGAGAAACTACTTTGTTTGTTTGCAATTAAAGTACATGAGAAACAATTTTATTATTCTTCCAATTGTGTAGATATTTATCTTTCCAGTGCTTTGGCTCATCTCCCGATATTCCTGCAGAATGTACATCATGCAGGGCAACCAATGCCAGGTGAAGAGCAGGAGGACATTGTGATGACCAGTACTCAGTGTAATTTATTGAATGTCACTTGCCCATTAAGTGGAAAGCCTGTCACTGAATTGGCACAACCTGTTCGCAGGTATATTGCAACGAAAGATTTTTTCATGAAGAACGAAGAATTAGATATATTTCTTTTACTGTTGAAAAATAAGTCTGCTCATCTATTCACTCTGTACTAACAGCATGGATGGTTACATGCTTGTGTTATTCTCTCTGGTTACATTTATCAAGTGGGTGCCTGAAAATGGAATGAGCTAGACTTTCACTTGTGATTTTTTTCCCTCGTAATAAATTGACAACCACCCTGAGTTAGACTTAGACTGGTGACATTGGGGATGTGAGAAAAAGTAAAGAGAATTTAGAGGAATAGTTTAAATCACATGCTGGCAAGATGACAAATTTCTTAACAACTAAATAATGTAGGATCAAACAGTTATCTTGCGAGCTTTATTAAGGTGCATGATAAATGGGTCTGGTACTCGCTATTTGAGTTGATCTAATAGATCTTGAACTTTAAAAGTGTCTAATAGAATCTGAACTTTTGATTCTATCTCTACCTTTTACAATTTGTAGTATGGAATGCAAGCATGTGTATGAAAAGGAGGCTGTGATGCAGTACCTAAGATCTAAGAATTCTCGAGCTCAATGCCCTGTAGCAGGTATCCAGGTCTTCTACATGTAATGTAGTACATACAAAATGTATAGATTGCTTTTCTAGTTTTCTCACTTGAACTCTTTGTCCTTGTAGCCTGTCCTAAGATGTTGCAGCCAGAAAAGGTCACGCCTGATCCGTTCTTACTGATTGAAATTGATGAACTGAGAAAAACGTCTAGGCACTCTGTGAGAATACAAGATTTTACAGAGCTTGATGCAGAATAG

mRNA sequence

ATGGCGTCGACTTCTGATACCCGTTCAAGTGCCGTTTCGGGCAGAATCAAATCCGCTGCCACTATGATGCACTCCGAGAATCAGTCCCTGCTTGCTGAAATACGCAAGTTGTTGATTATGATGAAGGAAATTGGGATGGATTTGGAGAGGGACAATCAGTCGACGATGGTCAAGAAGCTTGAAAATGCTGTTGTTGAGCTGTTGAGTACTTACGAAAACTGTGACAACTTTTCATCTGCGATTCAGTCGGTTGGAAATATATATGAACCAAGAGAAGAGTTAACAGATTTTGAGAAACTACTTGACGATGAAGTTGCAAAAGTCAGTGAAAATTCATCTTCAAATTTGCAGAACCATTCAATAATTCGGAAGTTTAGAGAAGCTATTTGGAATGTACATCATGCAGGGCAACCAATGCCAGGTGAAGAGCAGGAGGACATTGTGATGACCAGTACTCAGTGTAATTTATTGAATGTCACTTGCCCATTAAGTGGAAAGCCTGTCACTGAATTGGCACAACCTGTTCGCAGTATGGAATGCAAGCATGTGTATGAAAAGGAGGCTGTGATGCAGTACCTAAGATCTAAGAATTCTCGAGCTCAATGCCCTGTAGCAGCCTGTCCTAAGATGTTGCAGCCAGAAAAGGTCACGCCTGATCCGTTCTTACTGATTGAAATTGATGAACTGAGAAAAACGTCTAGGCACTCTGTGAGAATACAAGATTTTACAGAGCTTGATGCAGAATAG

Coding sequence (CDS)

ATGGCGTCGACTTCTGATACCCGTTCAAGTGCCGTTTCGGGCAGAATCAAATCCGCTGCCACTATGATGCACTCCGAGAATCAGTCCCTGCTTGCTGAAATACGCAAGTTGTTGATTATGATGAAGGAAATTGGGATGGATTTGGAGAGGGACAATCAGTCGACGATGGTCAAGAAGCTTGAAAATGCTGTTGTTGAGCTGTTGAGTACTTACGAAAACTGTGACAACTTTTCATCTGCGATTCAGTCGGTTGGAAATATATATGAACCAAGAGAAGAGTTAACAGATTTTGAGAAACTACTTGACGATGAAGTTGCAAAAGTCAGTGAAAATTCATCTTCAAATTTGCAGAACCATTCAATAATTCGGAAGTTTAGAGAAGCTATTTGGAATGTACATCATGCAGGGCAACCAATGCCAGGTGAAGAGCAGGAGGACATTGTGATGACCAGTACTCAGTGTAATTTATTGAATGTCACTTGCCCATTAAGTGGAAAGCCTGTCACTGAATTGGCACAACCTGTTCGCAGTATGGAATGCAAGCATGTGTATGAAAAGGAGGCTGTGATGCAGTACCTAAGATCTAAGAATTCTCGAGCTCAATGCCCTGTAGCAGCCTGTCCTAAGATGTTGCAGCCAGAAAAGGTCACGCCTGATCCGTTCTTACTGATTGAAATTGATGAACTGAGAAAAACGTCTAGGCACTCTGTGAGAATACAAGATTTTACAGAGCTTGATGCAGAATAG

Protein sequence

MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKLENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHSIIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMECKHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQDFTELDAE

Homology

[Close]

BLAST of Moc11g10090 vs. NCBI nr
Match: XP_022139610.1 (E3 SUMO-protein ligase MMS21 isoform X1 [Momordica charantia])

HSP 1 Score: 481.1 bits (1237), Expect = 5.8e-132
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60
           MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL
Sbjct: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60

Query: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120
           ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS
Sbjct: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180
           IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC
Sbjct: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180

Query: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240
           KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ
Sbjct: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240

Query: 241 DFTELDAE 249
           DFTELDAE
Sbjct: 241 DFTELDAE 248

[Close]

BLAST of Moc11g10090 vs. NCBI nr
Match: XP_022941288.1 (E3 SUMO-protein ligase MMS21 [Cucurbita moschata])

HSP 1 Score: 423.3 bits (1087), Expect = 1.4e-114
Identity = 215/248 (86.69%), Postives = 234/248 (94.35%), Query Frame = 0


Query: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60
           MASTS++ S+ VS RIKSAAT+M+SENQSLLAE+RK LIMMKEIG++LERDNQS MVK+L
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVELERDNQSRMVKEL 60

Query: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120
           EN+VVELL TYE C NFSSAIQSVGNIYEP+EELTDFEKLLDDEVAKVSENSSSNLQNHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180
           IIR+FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELA+PVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240
           KHVYEK A+MQYL+SK SRAQCPVAACPKMLQPEKV  DPFL IEIDELRKTS+HSVRIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTELDAE 249
           DFTE+DA+
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of Moc11g10090 vs. NCBI nr
Match: XP_023525588.1 (E3 SUMO-protein ligase MMS21 [Cucurbita pepo subsp. pepo] >KAG7037426.1 E3 SUMO-protein ligase MMS21 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 422.9 bits (1086), Expect = 1.9e-114
Identity = 215/248 (86.69%), Postives = 234/248 (94.35%), Query Frame = 0


Query: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60
           MASTS++ S+ VS RIKSAAT+M+SENQSLLAE+RK LIMMKEIG+DLERDNQS MVK+L
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60

Query: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120
           EN+VVELL TYE C NFSSAIQSVGNIYEP+EELTDFEKLLD+EVAKVSENSSSNLQNHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHS 120

Query: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180
           IIR+FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELA+PVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240
           KHVYEK A+MQYL+SK SRAQCPVAACPKMLQPEKV  DPFL IEIDELRKTS+HSVRIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTELDAE 249
           DFTE+DA+
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of Moc11g10090 vs. NCBI nr
Match: KAG6607898.1 (E3 SUMO-protein ligase MMS21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 421.0 bits (1081), Expect = 7.1e-114
Identity = 214/248 (86.29%), Postives = 233/248 (93.95%), Query Frame = 0


Query: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60
           MASTS++ S+ VS RIKSAAT+M+SENQSLLAE+RK LIMMKEIG+DLERDNQS MVK+L
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60

Query: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120
           EN+VVELL TYE C NFSSAIQSVGNIYEP+EELTDFEKLLD+EVAKVSENSSSNL NHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLHNHS 120

Query: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180
           IIR+FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELA+PVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240
           KHVYEK A+MQYL+SK SRAQCPVAACPKMLQPEKV  DPFL IEIDELRKTS+HSVRIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTELDAE 249
           DFTE+DA+
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of Moc11g10090 vs. NCBI nr
Match: XP_022981951.1 (E3 SUMO-protein ligase MMS21 [Cucurbita maxima])

HSP 1 Score: 419.9 bits (1078), Expect = 1.6e-113
Identity = 216/250 (86.40%), Postives = 236/250 (94.40%), Query Frame = 0


Query: 1   MASTSDTRSSA--VSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVK 60
           MASTS++ S++  VS RIKSAAT+M+SENQSLLAE+RK LIMMKEIG+DLERDNQS MVK
Sbjct: 1   MASTSNSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVK 60

Query: 61  KLENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQN 120
           +LEN+VVELLSTYE C NFSSAIQSVGNIYEP+EELTDFEKLLD+EVAKVSENSSSNLQN
Sbjct: 61  ELENSVVELLSTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQN 120

Query: 121 HSIIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSM 180
           HSIIR+FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELA+PVRS 
Sbjct: 121 HSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRST 180

Query: 181 ECKHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVR 240
           ECKHVYEK A+MQYL+SK SRAQCPVAACPKMLQPEKV  DPFL IEIDELRKTS+HSVR
Sbjct: 181 ECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVR 240

Query: 241 IQDFTELDAE 249
           IQDFTE+DA+
Sbjct: 241 IQDFTEVDAD 250

[Close]

BLAST of Moc11g10090 vs. ExPASy Swiss-Prot
Match: Q8GYH7 (E3 SUMO-protein ligase MMS21 OS=Arabidopsis thaliana OX=3702 GN=MMS21 PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 4.2e-69
Identity = 139/247 (56.28%), Postives = 180/247 (72.87%), Query Frame = 0


Query: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60
           MAS S   S  V+GRI++A+ ++ S+N S LA+IRK + MMK I + LE++NQ+  VK L
Sbjct: 1   MASASS--SDGVAGRIQNASLVLVSDNSSTLADIRKAVAMMKNIAVQLEKENQTDKVKDL 60

Query: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120
           EN+V ELL  + +C++ S+AIQSV N Y+P E+LTDF+KLLDDE  K+    SS  QN  
Sbjct: 61  ENSVAELLDLHSDCNHRSTAIQSVANRYQPVEQLTDFKKLLDDEFTKLKATPSSVPQNDH 120

Query: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180
           ++R+FREA+WNVHHAG+PMPG++ EDIVMTSTQC LLN+TCPLSGKPVTELA PVRSM+C
Sbjct: 121 LMRQFREAVWNVHHAGEPMPGDDDEDIVMTSTQCPLLNMTCPLSGKPVTELADPVRSMDC 180

Query: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVR-- 240
           +HVYEK  ++ Y+   N  A CPVA C   LQ  KV  D  L  EI+E+R  ++ S R  
Sbjct: 181 RHVYEKSVILHYI-VNNPNANCPVAGCRGKLQNSKVICDAMLKFEIEEMRSLNKQSNRAE 240

Query: 241 -IQDFTE 245
            I+DFTE
Sbjct: 241 VIEDFTE 244

[Close]

BLAST of Moc11g10090 vs. ExPASy TrEMBL
Match: A0A6J1CCS6 (E3 SUMO-protein ligase MMS21 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010470 PE=3 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 2.8e-132
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60
           MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL
Sbjct: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60

Query: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120
           ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS
Sbjct: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180
           IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC
Sbjct: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180

Query: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240
           KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ
Sbjct: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240

Query: 241 DFTELDAE 249
           DFTELDAE
Sbjct: 241 DFTELDAE 248

[Close]

BLAST of Moc11g10090 vs. ExPASy TrEMBL
Match: A0A6J1FRP6 (E3 SUMO-protein ligase MMS21 OS=Cucurbita moschata OX=3662 GN=LOC111446631 PE=3 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 6.9e-115
Identity = 215/248 (86.69%), Postives = 234/248 (94.35%), Query Frame = 0


Query: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60
           MASTS++ S+ VS RIKSAAT+M+SENQSLLAE+RK LIMMKEIG++LERDNQS MVK+L
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVELERDNQSRMVKEL 60

Query: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120
           EN+VVELL TYE C NFSSAIQSVGNIYEP+EELTDFEKLLDDEVAKVSENSSSNLQNHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180
           IIR+FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELA+PVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240
           KHVYEK A+MQYL+SK SRAQCPVAACPKMLQPEKV  DPFL IEIDELRKTS+HSVRIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTELDAE 249
           DFTE+DA+
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of Moc11g10090 vs. ExPASy TrEMBL
Match: A0A6J1IXZ2 (E3 SUMO-protein ligase MMS21 OS=Cucurbita maxima OX=3661 GN=LOC111480943 PE=3 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 7.6e-114
Identity = 216/250 (86.40%), Postives = 236/250 (94.40%), Query Frame = 0


Query: 1   MASTSDTRSSA--VSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVK 60
           MASTS++ S++  VS RIKSAAT+M+SENQSLLAE+RK LIMMKEIG+DLERDNQS MVK
Sbjct: 1   MASTSNSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVK 60

Query: 61  KLENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQN 120
           +LEN+VVELLSTYE C NFSSAIQSVGNIYEP+EELTDFEKLLD+EVAKVSENSSSNLQN
Sbjct: 61  ELENSVVELLSTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQN 120

Query: 121 HSIIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSM 180
           HSIIR+FREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTELA+PVRS 
Sbjct: 121 HSIIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRST 180

Query: 181 ECKHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVR 240
           ECKHVYEK A+MQYL+SK SRAQCPVAACPKMLQPEKV  DPFL IEIDELRKTS+HSVR
Sbjct: 181 ECKHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVR 240

Query: 241 IQDFTELDAE 249
           IQDFTE+DA+
Sbjct: 241 IQDFTEVDAD 250

[Close]

BLAST of Moc11g10090 vs. ExPASy TrEMBL
Match: A0A6J1CD70 (E3 SUMO-protein ligase MMS21 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010470 PE=3 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 4.9e-113
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 40  MMKEIGMDLERDNQSTMVKKLENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEK 99
           MMKEIGMDLERDNQSTMVKKLENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEK
Sbjct: 1   MMKEIGMDLERDNQSTMVKKLENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEK 60

Query: 100 LLDDEVAKVSENSSSNLQNHSIIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNV 159
           LLDDEVAKVSENSSSNLQNHSIIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNV
Sbjct: 61  LLDDEVAKVSENSSSNLQNHSIIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNV 120

Query: 160 TCPLSGKPVTELAQPVRSMECKHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPD 219
           TCPLSGKPVTELAQPVRSMECKHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPD
Sbjct: 121 TCPLSGKPVTELAQPVRSMECKHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPD 180

Query: 220 PFLLIEIDELRKTSRHSVRIQDFTELDAE 249
           PFLLIEIDELRKTSRHSVRIQDFTELDAE
Sbjct: 181 PFLLIEIDELRKTSRHSVRIQDFTELDAE 209

[Close]

BLAST of Moc11g10090 vs. ExPASy TrEMBL
Match: A0A5D3DWX9 (E3 SUMO-protein ligase MMS21 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001530 PE=3 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 1.3e-110
Identity = 206/248 (83.06%), Postives = 227/248 (91.53%), Query Frame = 0


Query: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60
           MAS SD+RS+ V+GRIKSAAT+MHSENQSLLAE+RK LIMMKEIG+DLE++NQ+ MVK+L
Sbjct: 1   MASASDSRSTGVTGRIKSAATIMHSENQSLLAELRKTLIMMKEIGVDLEKENQNKMVKEL 60

Query: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120
           E ++VELLS YENC+NFSSAIQSVGN YEP+EELTDFEKLLDDEVAKVS NSSSN  NH 
Sbjct: 61  EKSMVELLSAYENCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKVSVNSSSNFANHP 120

Query: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180
           IIR+FREAIWNVHHAGQPM GEEQED+VMTSTQCNLLNVTCPLSGKPVTELA+PVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMAGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSAEC 180

Query: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240
           KH+YEK A+MQYL SK SRAQCPVAACPKMLQP+KV  DPFL IEIDELRK SRHS RIQ
Sbjct: 181 KHIYEKAAIMQYLNSKKSRAQCPVAACPKMLQPDKVVLDPFLEIEIDELRKMSRHSERIQ 240

Query: 241 DFTELDAE 249
           DFTELDA+
Sbjct: 241 DFTELDAD 248

[Close]

BLAST of Moc11g10090 vs. TAIR 10
Match: AT3G15150.1 (RING/U-box superfamily protein )

HSP 1 Score: 262.7 bits (670), Expect = 3.0e-70
Identity = 139/247 (56.28%), Postives = 180/247 (72.87%), Query Frame = 0


Query: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60
           MAS S   S  V+GRI++A+ ++ S+N S LA+IRK + MMK I + LE++NQ+  VK L
Sbjct: 1   MASASS--SDGVAGRIQNASLVLVSDNSSTLADIRKAVAMMKNIAVQLEKENQTDKVKDL 60

Query: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120
           EN+V ELL  + +C++ S+AIQSV N Y+P E+LTDF+KLLDDE  K+    SS  QN  
Sbjct: 61  ENSVAELLDLHSDCNHRSTAIQSVANRYQPVEQLTDFKKLLDDEFTKLKATPSSVPQNDH 120

Query: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180
           ++R+FREA+WNVHHAG+PMPG++ EDIVMTSTQC LLN+TCPLSGKPVTELA PVRSM+C
Sbjct: 121 LMRQFREAVWNVHHAGEPMPGDDDEDIVMTSTQCPLLNMTCPLSGKPVTELADPVRSMDC 180

Query: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVR-- 240
           +HVYEK  ++ Y+   N  A CPVA C   LQ  KV  D  L  EI+E+R  ++ S R  
Sbjct: 181 RHVYEKSVILHYI-VNNPNANCPVAGCRGKLQNSKVICDAMLKFEIEEMRSLNKQSNRAE 240

Query: 241 -IQDFTE 245
            I+DFTE
Sbjct: 241 VIEDFTE 244

The following BLAST results are available for this feature:

BLAST of Moc11g10090 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139610.1	5.8e-132	100.00	E3 SUMO-protein ligase MMS21 isoform X1 [Momordica charantia]	[more]
XP_022941288.1	1.4e-114	86.69	E3 SUMO-protein ligase MMS21 [Cucurbita moschata]	[more]
XP_023525588.1	1.9e-114	86.69	E3 SUMO-protein ligase MMS21 [Cucurbita pepo subsp. pepo] >KAG7037426.1 E3 SUMO-...	[more]
KAG6607898.1	7.1e-114	86.29	E3 SUMO-protein ligase MMS21, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022981951.1	1.6e-113	86.40	E3 SUMO-protein ligase MMS21 [Cucurbita maxima]	[more]

BLAST of Moc11g10090 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GYH7	4.2e-69	56.28	E3 SUMO-protein ligase MMS21 OS=Arabidopsis thaliana OX=3702 GN=MMS21 PE=1 SV=1	[more]

BLAST of Moc11g10090 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CCS6	2.8e-132	100.00	E3 SUMO-protein ligase MMS21 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1FRP6	6.9e-115	86.69	E3 SUMO-protein ligase MMS21 OS=Cucurbita moschata OX=3662 GN=LOC111446631 PE=3 ...	[more]
A0A6J1IXZ2	7.6e-114	86.40	E3 SUMO-protein ligase MMS21 OS=Cucurbita maxima OX=3661 GN=LOC111480943 PE=3 SV...	[more]
A0A6J1CD70	4.9e-113	100.00	E3 SUMO-protein ligase MMS21 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A5D3DWX9	1.3e-110	83.06	E3 SUMO-protein ligase MMS21 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]

BLAST of Moc11g10090 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15150.1	3.0e-70	56.28	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004181	Zinc finger, MIZ-type	PFAM	PF11789	zf-Nse	coord: 145..207 e-value: 5.9E-15 score: 54.9
IPR004181	Zinc finger, MIZ-type	CDD	cd16651	SPL-RING_NSE2	coord: 160..228 e-value: 7.04793E-23 score: 86.5296
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 153..239 e-value: 1.3E-18 score: 69.0
IPR026846	E3 SUMO-protein ligase Nse2 (Mms21)	PANTHER	PTHR21330	UNCHARACTERIZED	coord: 8..235
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 159..231

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc11g10090.1	Moc11g10090.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000724	double-strand break repair via homologous recombination
biological_process	GO:0060250	germ-line stem-cell niche homeostasis
biological_process	GO:0032876	negative regulation of DNA endoreduplication
biological_process	GO:0008284	positive regulation of cell population proliferation
biological_process	GO:0080038	positive regulation of cytokinin-activated signaling pathway
biological_process	GO:0045931	positive regulation of mitotic cell cycle
biological_process	GO:0016925	protein sumoylation
biological_process	GO:0048509	regulation of meristem development
biological_process	GO:0010082	regulation of root meristem growth
cellular_component	GO:0005634	nucleus
cellular_component	GO:0030915	Smc5-Smc6 complex
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0061665	SUMO ligase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0019789	SUMO transferase activity