Moc11g08220 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc11g08220
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	1-aminocyclopropane-1-carboxylate oxidase
Location	chr11: 5847769 .. 5849069 (+)
RNA-Seq Expression	Moc11g08220
Synteny	Moc11g08220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAATCCCCATTATTGACTTCTCAAAGCTTAATGGAGAAGAAAGAGCCAATACATTGGCTCAGATAGCCAATGGCTGTGAAGAATGGGGATTCTTTCAGGTTTCTTTTTTCTACTCGTTCGACTTATTTCAAGTTAGATTTGTGATTACTAGAAGCAGAGCGAAAAAAGAAGAAATAAAACTTTTCCCTCTCCCTAAGAAATAAAAGTAGGGGGTGGTGTTTTTAGACATCCTATTTCGTGCTAGAATTAGTTAAATGTTAATAATATTGTTGACTAGATATGGTACTATTTGACATTCAGCTGGTGAACCATGGGATTCCTGAGGAACTCTTGGAGAGGGTGAAGAGGGTGTGTTCAGAGTGCTACAAGCTGGAAAGAGAAGAGAGTTTCAAGAGTTCAAAACCTGTGAAGCTTCTGAATGATTTGCTGGAAAACAAGAGCGGTGAGAAGCTGGAGAATCTTGATTGGGAAGATGTCTTCCTTCTCCATGACGAAAACGACTGGCCCTCCAACATTCCTGGATTCAAGTAAGCAAGCATCTTATCTTACACTCCAAACTTTATGCTTTTTCATAAAAAGGAATTCAGTGAAAATATATACATTCTACAGTCACATTTTCTTAAAAGCAGAAAGTTGGTCTCAAAGCAACTAACGAGTGGTTCTTAATGATGTTGTTTATGAATCCAAATATGGCAGGGAAACCATGGCAGAGTACAGATCGGAGCTGAAGAAGCTAGCAAAAAAGGTGATGGCAGTGATGGATGAGAACTTAGGTTTACCTGAGGGATACATAAACGCTGCCTTCAATGGTGGCGAAGGGCTGGAGAAAGCCTTCTTTGGCACCAAAGTCAGCCACTACCCACCATGCCCACACCCAGAGCTGGTGAACGGCCTCCGTGCACACACTGATGCTGGCGGTGTCATCTTGCTCTTCCAAGACGACCAGGTGGGAGGCCTTCAGATCCTGAAAGATGGGCAGTGGATAGATGTGCAGCCATTACCCAACTCCATAGTCATCAACACAGGTGACCAGATTGAGGTCCTGAGCAATGGAAGATACAAAAGTGTCTGGCACCGGGTTTTGGCCATCCCAAATGGGAACAGGAGATCAATTGCTTCATTTTACAACCCATCAATGGAAGCCACCATAGCTCCTGCAGCACAGCTGGTGGATAAAGCAAACCAGGAGGTGGAACAAAGATATCCCAAGTTTGTGTTCGGAGACTACATGTCGGTTTATGCTGAACAGAAGTTCCTCCCAAAGGAACCAAGGTTCCAATCTGTGAGGGCAATGTGA

mRNA sequence

ATGGCAATCCCCATTATTGACTTCTCAAAGCTTAATGGAGAAGAAAGAGCCAATACATTGGCTCAGATAGCCAATGGCTGTGAAGAATGGGGATTCTTTCAGCTGGTGAACCATGGGATTCCTGAGGAACTCTTGGAGAGGGTGAAGAGGGTGTGTTCAGAGTGCTACAAGCTGGAAAGAGAAGAGAGTTTCAAGAGTTCAAAACCTGTGAAGCTTCTGAATGATTTGCTGGAAAACAAGAGCGGTGAGAAGCTGGAGAATCTTGATTGGGAAGATGTCTTCCTTCTCCATGACGAAAACGACTGGCCCTCCAACATTCCTGGATTCAAGGAAACCATGGCAGAGTACAGATCGGAGCTGAAGAAGCTAGCAAAAAAGGTGATGGCAGTGATGGATGAGAACTTAGGTTTACCTGAGGGATACATAAACGCTGCCTTCAATGGTGGCGAAGGGCTGGAGAAAGCCTTCTTTGGCACCAAAGTCAGCCACTACCCACCATGCCCACACCCAGAGCTGGTGAACGGCCTCCGTGCACACACTGATGCTGGCGGTGTCATCTTGCTCTTCCAAGACGACCAGGTGGGAGGCCTTCAGATCCTGAAAGATGGGCAGTGGATAGATGTGCAGCCATTACCCAACTCCATAGTCATCAACACAGGTGACCAGATTGAGGTCCTGAGCAATGGAAGATACAAAAGTGTCTGGCACCGGGTTTTGGCCATCCCAAATGGGAACAGGAGATCAATTGCTTCATTTTACAACCCATCAATGGAAGCCACCATAGCTCCTGCAGCACAGCTGGTGGATAAAGCAAACCAGGAGGTGGAACAAAGATATCCCAAGTTTGTGTTCGGAGACTACATGTCGGTTTATGCTGAACAGAAGTTCCTCCCAAAGGAACCAAGGTTCCAATCTGTGAGGGCAATGTGA

Coding sequence (CDS)

ATGGCAATCCCCATTATTGACTTCTCAAAGCTTAATGGAGAAGAAAGAGCCAATACATTGGCTCAGATAGCCAATGGCTGTGAAGAATGGGGATTCTTTCAGCTGGTGAACCATGGGATTCCTGAGGAACTCTTGGAGAGGGTGAAGAGGGTGTGTTCAGAGTGCTACAAGCTGGAAAGAGAAGAGAGTTTCAAGAGTTCAAAACCTGTGAAGCTTCTGAATGATTTGCTGGAAAACAAGAGCGGTGAGAAGCTGGAGAATCTTGATTGGGAAGATGTCTTCCTTCTCCATGACGAAAACGACTGGCCCTCCAACATTCCTGGATTCAAGGAAACCATGGCAGAGTACAGATCGGAGCTGAAGAAGCTAGCAAAAAAGGTGATGGCAGTGATGGATGAGAACTTAGGTTTACCTGAGGGATACATAAACGCTGCCTTCAATGGTGGCGAAGGGCTGGAGAAAGCCTTCTTTGGCACCAAAGTCAGCCACTACCCACCATGCCCACACCCAGAGCTGGTGAACGGCCTCCGTGCACACACTGATGCTGGCGGTGTCATCTTGCTCTTCCAAGACGACCAGGTGGGAGGCCTTCAGATCCTGAAAGATGGGCAGTGGATAGATGTGCAGCCATTACCCAACTCCATAGTCATCAACACAGGTGACCAGATTGAGGTCCTGAGCAATGGAAGATACAAAAGTGTCTGGCACCGGGTTTTGGCCATCCCAAATGGGAACAGGAGATCAATTGCTTCATTTTACAACCCATCAATGGAAGCCACCATAGCTCCTGCAGCACAGCTGGTGGATAAAGCAAACCAGGAGGTGGAACAAAGATATCCCAAGTTTGTGTTCGGAGACTACATGTCGGTTTATGCTGAACAGAAGTTCCTCCCAAAGGAACCAAGGTTCCAATCTGTGAGGGCAATGTGA

Protein sequence

MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLEREESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSELKKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHTDAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLAIPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLPKEPRFQSVRAM

Homology

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BLAST of Moc11g08220 vs. NCBI nr
Match: XP_022134061.1 (1-aminocyclopropane-1-carboxylate oxidase 5 [Momordica charantia])

HSP 1 Score: 637.9 bits (1644), Expect = 4.6e-179
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60
           MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER
Sbjct: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSEL 120

Query: 121 KKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 IPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLPKE 300
           IPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 IPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQSVRAM 310
           PRFQSVRAM
Sbjct: 301 PRFQSVRAM 309

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BLAST of Moc11g08220 vs. NCBI nr
Match: XP_004142637.1 (1-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis sativus] >XP_031745860.1 1-aminocyclopropane-1-carboxylate oxidase 5-like [Cucumis sativus] >KAE8637421.1 hypothetical protein CSA_021607 [Cucumis sativus] >KGN54542.1 hypothetical protein Csa_012615 [Cucumis sativus])

HSP 1 Score: 608.2 bits (1567), Expect = 3.9e-170
Identity = 290/309 (93.85%), Postives = 302/309 (97.73%), Query Frame = 0


Query: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60
           MAIP+IDFSKLNG+ERA TLAQIANGCEEWGFFQLVNHGIPEELLERVK+V SECYK+ER
Sbjct: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHD+N+WPSN+PGFKETM EYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120

Query: 121 KKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           +KLA+KVMAVMDENLGLPEGYI AAFNGGEGLEKAFFGTK+SHYPPCPHPELVNGLRAHT
Sbjct: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQP PNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 IPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLPKE 300
            PNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVE+ YPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQSVRAM 310
           PRFQ+VRAM
Sbjct: 301 PRFQAVRAM 309

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BLAST of Moc11g08220 vs. NCBI nr
Match: KAG7024737.1 (1-aminocyclopropane-1-carboxylate oxidase 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 607.8 bits (1566), Expect = 5.1e-170
Identity = 291/309 (94.17%), Postives = 302/309 (97.73%), Query Frame = 0


Query: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60
           MAIP+IDFSKL+GEERA TL QIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER
Sbjct: 31  MAIPVIDFSKLDGEERAKTLNQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 90

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHD N+WPSNIPGFKETMAEYRSEL
Sbjct: 91  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 150

Query: 121 KKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLA+KVMA+MDENLGLPEGYI AAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 151 KKLAEKVMALMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 210

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQPL NSIVINTGDQIEVLSNG+YKSVWHR+LA
Sbjct: 211 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLHNSIVINTGDQIEVLSNGKYKSVWHRILA 270

Query: 241 IPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLPKE 300
            PNGNRRSIASFYNPS+EATIAPAAQLVDKANQEVE+ YPKFVFGDYMSVYAEQKFLPKE
Sbjct: 271 TPNGNRRSIASFYNPSLEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 330

Query: 301 PRFQSVRAM 310
           PRFQ+VRA+
Sbjct: 331 PRFQAVRAV 339

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BLAST of Moc11g08220 vs. NCBI nr
Match: XP_022936989.1 (1-aminocyclopropane-1-carboxylate oxidase 5-like isoform X1 [Cucurbita moschata] >XP_022936990.1 1-aminocyclopropane-1-carboxylate oxidase 5-like isoform X2 [Cucurbita moschata] >KAG6591869.1 1-aminocyclopropane-1-carboxylate oxidase 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 607.8 bits (1566), Expect = 5.1e-170
Identity = 291/309 (94.17%), Postives = 302/309 (97.73%), Query Frame = 0


Query: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60
           MAIP+IDFSKL+GEERA TL QIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER
Sbjct: 1   MAIPVIDFSKLDGEERAKTLNQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHD N+WPSNIPGFKETMAEYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120

Query: 121 KKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLA+KVMA+MDENLGLPEGYI AAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMALMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQPL NSIVINTGDQIEVLSNG+YKSVWHR+LA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLHNSIVINTGDQIEVLSNGKYKSVWHRILA 240

Query: 241 IPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLPKE 300
            PNGNRRSIASFYNPS+EATIAPAAQLVDKANQEVE+ YPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 TPNGNRRSIASFYNPSLEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQSVRAM 310
           PRFQ+VRA+
Sbjct: 301 PRFQAVRAV 309

[Close]

BLAST of Moc11g08220 vs. NCBI nr
Match: XP_008444197.1 (PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis melo] >KAA0052209.1 1-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis melo var. makuwa] >TYK01776.1 1-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 607.4 bits (1565), Expect = 6.6e-170
Identity = 291/309 (94.17%), Postives = 301/309 (97.41%), Query Frame = 0


Query: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60
           MAIP+IDFSKLNGEERA TLAQIANGCEEWGFFQLVNHGIPEELLERVK+V SECYK+ER
Sbjct: 1   MAIPVIDFSKLNGEERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSEL 120
           EESFKSSKPVKLLND LENKSGEKLENLDWEDVFLLHD+N+WPSN+PGFKETM EYRSEL
Sbjct: 61  EESFKSSKPVKLLNDWLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120

Query: 121 KKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLA+KVMAVMDENLGLPEGYI AAFN GEGLEKAFFGTK+SHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNSGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 IPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLPKE 300
            PNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVE+ YPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQSVRAM 310
           PRFQ+VRAM
Sbjct: 301 PRFQAVRAM 309

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BLAST of Moc11g08220 vs. ExPASy Swiss-Prot
Match: Q0WPW4 (1-aminocyclopropane-1-carboxylate oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g77330 PE=2 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 8.0e-134
Identity = 229/313 (73.16%), Postives = 266/313 (84.98%), Query Frame = 0


Query: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60
           MAIP+IDFSKLNGEER  TL++IA  CEEWGFFQLVNHGIP ELL +VK++ S+CYK ER
Sbjct: 1   MAIPVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLL--HDENDWPSNIPGFKETMAEYRS 120
           EE+FK+S PVKLLN+L++  SGEKLEN+DWEDVF L  H++N+WPSNI   KETM EYR 
Sbjct: 61  EEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLLDHNQNEWPSNI---KETMGEYRE 120

Query: 121 ELKKLAKKVMAVMDENLGLPEGYINAAFNGG--EGLEKAFFGTKVSHYPPCPHPELVNGL 180
           E++KLA K+M VMDENLGLP+GYI  AFN G  +G E AFFGTKVSHYPPCPHPELVNGL
Sbjct: 121 EVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPELVNGL 180

Query: 181 RAHTDAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWH 240
           RAHTDAGGV+LLFQDD+  GLQ+LKDG+WIDVQPLPN+IVINTGDQIEVLSNGRYKS WH
Sbjct: 181 RAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNGRYKSAWH 240

Query: 241 RVLAIPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKF 300
           RVLA   GNRRSIASFYNPS +A I PAA   ++ +   E++YPKFVFGDYM VYA QKF
Sbjct: 241 RVLAREEGNRRSIASFYNPSYKAAIGPAAVAEEEGS---EKKYPKFVFGDYMDVYANQKF 300

Query: 301 LPKEPRFQSVRAM 310
           +PKEPRF +V+++
Sbjct: 301 MPKEPRFLAVKSL 307

[Close]

BLAST of Moc11g08220 vs. ExPASy Swiss-Prot
Match: Q9FR99 (1-aminocyclopropane-1-carboxylate oxidase OS=Musa acuminata OX=4641 GN=MAO1B PE=3 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 5.0e-128
Identity = 223/311 (71.70%), Postives = 264/311 (84.89%), Query Frame = 0


Query: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60
           MAIP+IDFSKL+G+ERA T+A+IANGCEEWGFFQLVNHGIP ELLERVK+V SECYKL R
Sbjct: 1   MAIPVIDFSKLDGKERAETMARIANGCEEWGFFQLVNHGIPVELLERVKKVSSECYKL-R 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSEL 120
           EE F+ SKPV+LL+ L++   G++L+N+DWEDVF+L D+N+WPSN P F+ETM EYR E+
Sbjct: 61  EERFEGSKPVQLLDTLVKEGDGQRLDNVDWEDVFVLQDDNEWPSNPPDFEETMKEYREEI 120

Query: 121 KKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           +KLA+K+M VMDENLG  +G I  AF+ G+G    FFGTKVSHYPPCP  +LV GLRAHT
Sbjct: 121 RKLAEKMMEVMDENLGFEKGCIKKAFS-GDGQHPPFFGTKVSHYPPCPRLDLVKGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQ+LKDG+WIDVQPL ++IVINTGDQIEVLSNGRYKS WHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQMLKDGRWIDVQPLADAIVINTGDQIEVLSNGRYKSAWHRVLA 240

Query: 241 IPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEV--EQRYPKFVFGDYMSVYAEQKFLP 300
             +GNRRSIASFYNPS++ATIAPAA     A +E      YPKF+FGDYM VYA+QK+ P
Sbjct: 241 TSHGNRRSIASFYNPSLKATIAPAA---GAATEEAAPPALYPKFLFGDYMDVYAKQKYEP 300

Query: 301 KEPRFQSVRAM 310
           KEPRF++VRA+
Sbjct: 301 KEPRFEAVRAI 306

[Close]

BLAST of Moc11g08220 vs. ExPASy Swiss-Prot
Match: P31237 (1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO PE=2 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 9.2e-90
Identity = 163/313 (52.08%), Postives = 219/313 (69.97%), Query Frame = 0


Query: 2   AIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLERE 61
           A P+ID  KLNGEERA T+ +I + CE WGFF+LVNHGI  EL++ V+R+  E Y    E
Sbjct: 3   AFPVIDMEKLNGEERAPTMEKIKDACENWGFFELVNHGISHELMDTVERLTKEHYNKCME 62

Query: 62  ESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLH-----DENDWPSNIPGFKETMAEY 121
           + FK     K L  +       ++ +LDWE  F L      + ++ P      ++ M E+
Sbjct: 63  QRFKEMVATKGLEAV-----QSEINDLDWESTFFLRHLPVSNISEIPDLEQDHRKAMKEF 122

Query: 122 RSELKKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGL 181
             +L+KLA++++ ++ EN+GL +GY+  AF G +G     FGTKVS+YPPCP PEL+ GL
Sbjct: 123 AEKLEKLAEQLLDLLCENVGLEKGYLKKAFYGSKG---PTFGTKVSNYPPCPRPELIKGL 182

Query: 182 RAHTDAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWH 241
           RAHTDAGG+ILLFQD++V GLQ+LKDG+WIDV P+ +SIVIN GDQ+EV++NG+YKSV H
Sbjct: 183 RAHTDAGGIILLFQDNKVSGLQLLKDGEWIDVPPMKHSIVINIGDQLEVITNGKYKSVMH 242

Query: 242 RVLAIPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKF 301
           RV+A P+GNR SIASFYNP  +A + PA  LVDK   + +Q YPKFVF DYM +YA  KF
Sbjct: 243 RVIAQPDGNRMSIASFYNPGSDAVMYPAPALVDKEEDQQKQVYPKFVFEDYMKLYAGLKF 302

Query: 302 LPKEPRFQSVRAM 310
             KEPRF++++AM
Sbjct: 303 QAKEPRFEAMKAM 307

[Close]

BLAST of Moc11g08220 vs. ExPASy Swiss-Prot
Match: P05116 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Solanum lycopersicum OX=4081 GN=ACO1 PE=2 SV=2)

HSP 1 Score: 322.0 bits (824), Expect = 7.3e-87
Identity = 162/315 (51.43%), Postives = 219/315 (69.52%), Query Frame = 0


Query: 4   PIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLEREES 63
           PII+  KLNG+ERANT+  I + CE WGFF+LVNHGIP E+++ V+++    YK   E+ 
Sbjct: 5   PIINLEKLNGDERANTMEMIKDACENWGFFELVNHGIPHEVMDTVEKMTKGHYKKCMEQR 64

Query: 64  FKSSKPVKLLNDLLENKSGEKLE----NLDWEDVFLL-HDENDWPSNIPG----FKETMA 123
           FK         +L+ +K  E ++    +LDWE  F L H      S +P     ++E M 
Sbjct: 65  FK---------ELVASKGLEAVQAEVTDLDWESTFFLRHLPTSNISQVPDLDEEYREVMR 124

Query: 124 EYRSELKKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVN 183
           ++   L+KLA++++ ++ ENLGL +GY+  AF G +G     FGTKVS+YPPCP P+L+ 
Sbjct: 125 DFAKRLEKLAEELLDLLCENLGLEKGYLKNAFYGSKGPN---FGTKVSNYPPCPKPDLIK 184

Query: 184 GLRAHTDAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSV 243
           GLRAHTDAGG+ILLFQDD+V GLQ+LKD QWIDV P+ +SIV+N GDQ+EV++NG+YKSV
Sbjct: 185 GLRAHTDAGGIILLFQDDKVSGLQLLKDEQWIDVPPMRHSIVVNLGDQLEVITNGKYKSV 244

Query: 244 WHRVLAIPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQ 303
            HRV+A  +G R S+ASFYNP  +A I PA  LV+K  +E  Q YPKFVF DYM +YA  
Sbjct: 245 LHRVIAQTDGTRMSLASFYNPGSDAVIYPAKTLVEKEAEESTQVYPKFVFDDYMKLYAGL 304

Query: 304 KFLPKEPRFQSVRAM 310
           KF  KEPRF++++AM
Sbjct: 305 KFQAKEPRFEAMKAM 307

[Close]

BLAST of Moc11g08220 vs. ExPASy Swiss-Prot
Match: Q00985 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 2.8e-86
Identity = 158/310 (50.97%), Postives = 220/310 (70.97%), Query Frame = 0


Query: 4   PIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLEREES 63
           P++D S +NGEERA TL +I + CE WGFF+LVNHG+  ELL+ V+++  + YK   E+ 
Sbjct: 5   PVVDLSLVNGEERAATLEKINDACENWGFFELVNHGMSTELLDTVEKMTKDHYKKTMEQR 64

Query: 64  FKSSKPVKLLNDLLENKSGEKLENLDWEDVFLL-HDENDWPSNIPG----FKETMAEYRS 123
           FK     K L+D+       ++ +LDWE  F L H  +   S IP     +++TM E+  
Sbjct: 65  FKEMVAAKGLDDV-----QSEIHDLDWESTFFLRHLPSSNISEIPDLEEEYRKTMKEFAV 124

Query: 124 ELKKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRA 183
           EL+KLA+K++ ++ ENLGL +GY+   F G +G     FGTKVS+YPPCP P+L+ GLRA
Sbjct: 125 ELEKLAEKLLDLLCENLGLEKGYLKKVFYGSKGPN---FGTKVSNYPPCPKPDLIKGLRA 184

Query: 184 HTDAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRV 243
           H+DAGG+ILLFQDD+V GLQ+LKDG+W+DV P+ +SIVIN GDQIEV++NG+YKSV HRV
Sbjct: 185 HSDAGGIILLFQDDKVSGLQLLKDGEWVDVPPMHHSIVINLGDQIEVITNGKYKSVMHRV 244

Query: 244 LAIPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLP 303
           +A  +G R SIASFYNP  ++ I+PA  +++K  ++    YPKFVF DYM +Y+  KF  
Sbjct: 245 IAQSDGTRMSIASFYNPGNDSFISPAPAVLEKKTEDA-PTYPKFVFDDYMKLYSGLKFQA 304

Query: 304 KEPRFQSVRA 309
           KEPRF++++A
Sbjct: 305 KEPRFEAMKA 305

[Close]

BLAST of Moc11g08220 vs. ExPASy TrEMBL
Match: A0A6J1BWX3 (1-aminocyclopropane-1-carboxylate oxidase 5 OS=Momordica charantia OX=3673 GN=LOC111006422 PE=3 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.2e-179
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60
           MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER
Sbjct: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSEL 120

Query: 121 KKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 IPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLPKE 300
           IPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 IPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQSVRAM 310
           PRFQSVRAM
Sbjct: 301 PRFQSVRAM 309

[Close]

BLAST of Moc11g08220 vs. ExPASy TrEMBL
Match: A0A0A0L1U8 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G361270 PE=3 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 1.9e-170
Identity = 290/309 (93.85%), Postives = 302/309 (97.73%), Query Frame = 0


Query: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60
           MAIP+IDFSKLNG+ERA TLAQIANGCEEWGFFQLVNHGIPEELLERVK+V SECYK+ER
Sbjct: 1   MAIPVIDFSKLNGQERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHD+N+WPSN+PGFKETM EYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120

Query: 121 KKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           +KLA+KVMAVMDENLGLPEGYI AAFNGGEGLEKAFFGTK+SHYPPCPHPELVNGLRAHT
Sbjct: 121 RKLAEKVMAVMDENLGLPEGYIKAAFNGGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQP PNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPFPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 IPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLPKE 300
            PNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVE+ YPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQSVRAM 310
           PRFQ+VRAM
Sbjct: 301 PRFQAVRAM 309

[Close]

BLAST of Moc11g08220 vs. ExPASy TrEMBL
Match: A0A6J1FFA0 (1-aminocyclopropane-1-carboxylate oxidase 5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443415 PE=3 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 2.5e-170
Identity = 291/309 (94.17%), Postives = 302/309 (97.73%), Query Frame = 0


Query: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60
           MAIP+IDFSKL+GEERA TL QIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER
Sbjct: 1   MAIPVIDFSKLDGEERAKTLNQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSEL 120
           EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHD N+WPSNIPGFKETMAEYRSEL
Sbjct: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDNNEWPSNIPGFKETMAEYRSEL 120

Query: 121 KKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLA+KVMA+MDENLGLPEGYI AAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMALMDENLGLPEGYIKAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQPL NSIVINTGDQIEVLSNG+YKSVWHR+LA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLHNSIVINTGDQIEVLSNGKYKSVWHRILA 240

Query: 241 IPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLPKE 300
            PNGNRRSIASFYNPS+EATIAPAAQLVDKANQEVE+ YPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 TPNGNRRSIASFYNPSLEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQSVRAM 310
           PRFQ+VRA+
Sbjct: 301 PRFQAVRAV 309

[Close]

BLAST of Moc11g08220 vs. ExPASy TrEMBL
Match: A0A5A7UFF3 (1-aminocyclopropane-1-carboxylate oxidase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold113G00040 PE=3 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.2e-170
Identity = 291/309 (94.17%), Postives = 301/309 (97.41%), Query Frame = 0


Query: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60
           MAIP+IDFSKLNGEERA TLAQIANGCEEWGFFQLVNHGIPEELLERVK+V SECYK+ER
Sbjct: 1   MAIPVIDFSKLNGEERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSEL 120
           EESFKSSKPVKLLND LENKSGEKLENLDWEDVFLLHD+N+WPSN+PGFKETM EYRSEL
Sbjct: 61  EESFKSSKPVKLLNDWLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120

Query: 121 KKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLA+KVMAVMDENLGLPEGYI AAFN GEGLEKAFFGTK+SHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNSGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 IPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLPKE 300
            PNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVE+ YPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQSVRAM 310
           PRFQ+VRAM
Sbjct: 301 PRFQAVRAM 309

[Close]

BLAST of Moc11g08220 vs. ExPASy TrEMBL
Match: A0A1S3B9W8 (1-aminocyclopropane-1-carboxylate oxidase 5 OS=Cucumis melo OX=3656 GN=LOC103487610 PE=3 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.2e-170
Identity = 291/309 (94.17%), Postives = 301/309 (97.41%), Query Frame = 0


Query: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60
           MAIP+IDFSKLNGEERA TLAQIANGCEEWGFFQLVNHGIPEELLERVK+V SECYK+ER
Sbjct: 1   MAIPVIDFSKLNGEERAKTLAQIANGCEEWGFFQLVNHGIPEELLERVKKVSSECYKVER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLHDENDWPSNIPGFKETMAEYRSEL 120
           EESFKSSKPVKLLND LENKSGEKLENLDWEDVFLLHD+N+WPSN+PGFKETM EYRSEL
Sbjct: 61  EESFKSSKPVKLLNDWLENKSGEKLENLDWEDVFLLHDDNEWPSNLPGFKETMREYRSEL 120

Query: 121 KKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRAHT 180
           KKLA+KVMAVMDENLGLPEGYI AAFN GEGLEKAFFGTK+SHYPPCPHPELVNGLRAHT
Sbjct: 121 KKLAEKVMAVMDENLGLPEGYIKAAFNSGEGLEKAFFGTKISHYPPCPHPELVNGLRAHT 180

Query: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240
           DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA
Sbjct: 181 DAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRVLA 240

Query: 241 IPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLPKE 300
            PNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVE+ YPKFVFGDYMSVYAEQKFLPKE
Sbjct: 241 SPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEEGYPKFVFGDYMSVYAEQKFLPKE 300

Query: 301 PRFQSVRAM 310
           PRFQ+VRAM
Sbjct: 301 PRFQAVRAM 309

[Close]

BLAST of Moc11g08220 vs. TAIR 10
Match: AT1G77330.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 478.0 bits (1229), Expect = 5.7e-135
Identity = 229/313 (73.16%), Postives = 266/313 (84.98%), Query Frame = 0


Query: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60
           MAIP+IDFSKLNGEER  TL++IA  CEEWGFFQLVNHGIP ELL +VK++ S+CYK ER
Sbjct: 1   MAIPVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTER 60

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLL--HDENDWPSNIPGFKETMAEYRS 120
           EE+FK+S PVKLLN+L++  SGEKLEN+DWEDVF L  H++N+WPSNI   KETM EYR 
Sbjct: 61  EEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLLDHNQNEWPSNI---KETMGEYRE 120

Query: 121 ELKKLAKKVMAVMDENLGLPEGYINAAFNGG--EGLEKAFFGTKVSHYPPCPHPELVNGL 180
           E++KLA K+M VMDENLGLP+GYI  AFN G  +G E AFFGTKVSHYPPCPHPELVNGL
Sbjct: 121 EVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPELVNGL 180

Query: 181 RAHTDAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWH 240
           RAHTDAGGV+LLFQDD+  GLQ+LKDG+WIDVQPLPN+IVINTGDQIEVLSNGRYKS WH
Sbjct: 181 RAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNGRYKSAWH 240

Query: 241 RVLAIPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKF 300
           RVLA   GNRRSIASFYNPS +A I PAA   ++ +   E++YPKFVFGDYM VYA QKF
Sbjct: 241 RVLAREEGNRRSIASFYNPSYKAAIGPAAVAEEEGS---EKKYPKFVFGDYMDVYANQKF 300

Query: 301 LPKEPRFQSVRAM 310
           +PKEPRF +V+++
Sbjct: 301 MPKEPRFLAVKSL 307

[Close]

BLAST of Moc11g08220 vs. TAIR 10
Match: AT1G62380.1 (ACC oxidase 2 )

HSP 1 Score: 315.8 bits (808), Expect = 3.7e-86
Identity = 154/316 (48.73%), Postives = 214/316 (67.72%), Query Frame = 0


Query: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60
           M  P++D SKLNGEER  T+A I   CE WGFF++VNHG+P +L+++++++  + YK  +
Sbjct: 5   MKFPVVDLSKLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKIEKMTKDHYKTCQ 64

Query: 61  EESFKSSKPVKLLNDLLENKSGEKL----ENLDWEDVFLLH-----DENDWPSNIPGFKE 120
           E+ F         ND+L++K  + L    E++DWE  F +      + ND       ++ 
Sbjct: 65  EQKF---------NDMLKSKGLDNLETEVEDVDWESTFYVRHLPQSNLNDISDVSDEYRT 124

Query: 121 TMAEYRSELKKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPE 180
            M ++   L+ LA+ ++ ++ ENLGL +GY+   F+G +G     FGTKVS+YPPCP PE
Sbjct: 125 AMKDFGKRLENLAEDLLDLLCENLGLEKGYLKKVFHGTKG---PTFGTKVSNYPPCPKPE 184

Query: 181 LVNGLRAHTDAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRY 240
           ++ GLRAHTDAGG+ILLFQDD+V GLQ+LKDG WIDV PL +SIVIN GDQ+EV++NG+Y
Sbjct: 185 MIKGLRAHTDAGGIILLFQDDKVSGLQLLKDGDWIDVPPLNHSIVINLGDQLEVITNGKY 244

Query: 241 KSVWHRVLAIPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVY 300
           KSV HRV+    GNR S+ASFYNP  +A I+PA  LV+K     +  YP FVF DYM +Y
Sbjct: 245 KSVLHRVVTQQEGNRMSVASFYNPGSDAEISPATSLVEK-----DSEYPSFVFDDYMKLY 303

Query: 301 AEQKFLPKEPRFQSVR 308
           A  KF PKEPRF +++
Sbjct: 305 AGVKFQPKEPRFAAMK 303

[Close]

BLAST of Moc11g08220 vs. TAIR 10
Match: AT1G12010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 315.5 bits (807), Expect = 4.9e-86
Identity = 157/309 (50.81%), Postives = 212/309 (68.61%), Query Frame = 0


Query: 4   PIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLEREES 63
           P+ID SKLNGEER  T+A I + C+ WGFF+LVNHG+P +L++ ++R+  E YK   E+ 
Sbjct: 8   PVIDLSKLNGEERDQTMALIDDACQNWGFFELVNHGLPYDLMDNIERMTKEHYKKHMEQK 67

Query: 64  FKSSKPVKLLNDLLENKSGEKLENLDWEDVFLLH-----DENDWPSNIPGFKETMAEYRS 123
           FK     K L D LE     ++E++DWE  F LH     +  D P     ++  M ++  
Sbjct: 68  FKEMLRSKGL-DTLET----EVEDVDWESTFYLHHLPQSNLYDIPDMSNEYRLAMKDFGK 127

Query: 124 ELKKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGLRA 183
            L+ LA++++ ++ ENLGL +GY+   F+G  G     F TK+S+YPPCP PE++ GLRA
Sbjct: 128 RLEILAEELLDLLCENLGLEKGYLKKVFHGTTG---PTFATKLSNYPPCPKPEMIKGLRA 187

Query: 184 HTDAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWHRV 243
           HTDAGG+ILLFQDD+V GLQ+LKDG W+DV PL +SIVIN GDQ+EV++NG+YKSV HRV
Sbjct: 188 HTDAGGLILLFQDDKVSGLQLLKDGDWVDVPPLKHSIVINLGDQLEVITNGKYKSVMHRV 247

Query: 244 LAIPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPKFVFGDYMSVYAEQKFLP 303
           +    GNR SIASFYNP  +A I+PA  LVDK     + +YP FVF DYM +YA  KF  
Sbjct: 248 MTQKEGNRMSIASFYNPGSDAEISPATSLVDK-----DSKYPSFVFDDYMKLYAGLKFQA 303

Query: 304 KEPRFQSVR 308
           KEPRF++++
Sbjct: 308 KEPRFEAMK 303

[Close]

BLAST of Moc11g08220 vs. TAIR 10
Match: AT1G05010.1 (ethylene-forming enzyme )

HSP 1 Score: 309.7 bits (792), Expect = 2.7e-84
Identity = 154/315 (48.89%), Postives = 222/315 (70.48%), Query Frame = 0


Query: 2   AIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLERE 61
           + PII+  KLNGEERA T+ +I + CE WGFF+ VNHGI  ELL++V+++  E YK   E
Sbjct: 3   SFPIINLEKLNGEERAITMEKIKDACENWGFFECVNHGISLELLDKVEKMTKEHYKKCME 62

Query: 62  ESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLL-----HDENDWPSNIPGFKETMAEY 121
           E FK S   + L+ L       ++ ++DWE  F L      + +D P     ++  M ++
Sbjct: 63  ERFKESIKNRGLDSL-----RSEVNDVDWESTFYLKHLPVSNISDVPDLDDDYRTLMKDF 122

Query: 122 RSELKKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNGL 181
             +++KL+++++ ++ ENLGL +GY+   F G    ++  FGTKVS+YPPCP+P+LV GL
Sbjct: 123 AGKIEKLSEELLDLLCENLGLEKGYLKKVFYGS---KRPTFGTKVSNYPPCPNPDLVKGL 182

Query: 182 RAHTDAGGVILLFQDDQVGGLQILKDGQWIDVQPLPNSIVINTGDQIEVLSNGRYKSVWH 241
           RAHTDAGG+ILLFQDD+V GLQ+LKDG+W+DV P+ +SIV+N GDQ+EV++NG+YKSV H
Sbjct: 183 RAHTDAGGIILLFQDDKVSGLQLLKDGEWVDVPPVKHSIVVNLGDQLEVITNGKYKSVEH 242

Query: 242 RVLAIPNG-NRRSIASFYNPSMEATIAPAAQLVDK-ANQEVEQRYPKFVFGDYMSVYAEQ 301
           RVL+  +G  R SIASFYNP  ++ I PA +L+ K A +E ++ YP+FVF DYM +Y+  
Sbjct: 243 RVLSQTDGEGRMSIASFYNPGSDSVIFPAPELIGKEAEKEKKENYPRFVFEDYMKLYSAV 302

Query: 302 KFLPKEPRFQSVRAM 310
           KF  KEPRF++++AM
Sbjct: 303 KFQAKEPRFEAMKAM 309

[Close]

BLAST of Moc11g08220 vs. TAIR 10
Match: AT2G19590.1 (ACC oxidase 1 )

HSP 1 Score: 268.1 bits (684), Expect = 8.9e-72
Identity = 141/316 (44.62%), Postives = 204/316 (64.56%), Query Frame = 0


Query: 1   MAIPIIDFSKLNGEERANTLAQIANGCEEWGFFQLVNHGIPEELLERVKRVCSECYKLER 60
           M IP+IDF++L+GE+R+ T++ + + C++WGFF + NHGI +EL+E+VK++ +  Y+   
Sbjct: 9   MEIPVIDFAELDGEKRSKTMSLLDHACDKWGFFMVDNHGIDKELMEKVKKMINSHYEEHL 68

Query: 61  EESFKSSKPVKLLNDLLENKSGEKLENLDWEDVFLL-HDENDWPSNIPGFKE----TMAE 120
           +E F  S+ VK L++        K  + DWE  F + H        IP   E    TM E
Sbjct: 69  KEKFYQSEMVKALSE-------GKTSDADWESSFFISHKPTSNICQIPNISEELSKTMDE 128

Query: 121 YRSELKKLAKKVMAVMDENLGLPEGYINAAFNGGEGLEKAFFGTKVSHYPPCPHPELVNG 180
           Y  +L K A+++  +M ENLGL +  I  AF+G +G     FGTKV+ YP CP PEL+ G
Sbjct: 129 YVCQLHKFAERLSKLMCENLGLDQEDIMNAFSGPKG---PAFGTKVAKYPECPRPELMRG 188

Query: 181 LRAHTDAGGVILLFQDDQVGGLQILKDGQWIDVQPLP-NSIVINTGDQIEVLSNGRYKSV 240
           LR HTDAGG+ILL QDDQV GL+  KDG+W+ + P   N+I +NTGDQ+E+LSNGRYKSV
Sbjct: 189 LREHTDAGGIILLLQDDQVPGLEFFKDGKWVPIPPSKNNTIFVNTGDQLEILSNGRYKSV 248

Query: 241 WHRVLAIPNGNRRSIASFYNPSMEATIAPAAQLVDKANQEVEQRYPK-FVFGDYMSVYAE 300
            HRV+ + +G+R SIA+FYNP+ +A I+PA +L+          YP  + F DY+ +Y+ 
Sbjct: 249 VHRVMTVKHGSRLSIATFYNPAGDAIISPAPKLL----------YPSGYRFQDYLKLYST 304

Query: 301 QKFLPKEPRFQSVRAM 310
            KF  K PR ++++ M
Sbjct: 309 TKFGDKGPRLETMKKM 304

The following BLAST results are available for this feature:

BLAST of Moc11g08220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134061.1	4.6e-179	100.00	1-aminocyclopropane-1-carboxylate oxidase 5 [Momordica charantia]	[more]
XP_004142637.1	3.9e-170	93.85	1-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis sativus] >XP_031745860.1 1-...	[more]
KAG7024737.1	5.1e-170	94.17	1-aminocyclopropane-1-carboxylate oxidase 5, partial [Cucurbita argyrosperma sub...	[more]
XP_022936989.1	5.1e-170	94.17	1-aminocyclopropane-1-carboxylate oxidase 5-like isoform X1 [Cucurbita moschata]...	[more]
XP_008444197.1	6.6e-170	94.17	PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase 5 [Cucumis melo] >KAA005220...	[more]

BLAST of Moc11g08220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WPW4	8.0e-134	73.16	1-aminocyclopropane-1-carboxylate oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
Q9FR99	5.0e-128	71.70	1-aminocyclopropane-1-carboxylate oxidase OS=Musa acuminata OX=4641 GN=MAO1B PE=...	[more]
P31237	9.2e-90	52.08	1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO ...	[more]
P05116	7.3e-87	51.43	1-aminocyclopropane-1-carboxylate oxidase 1 OS=Solanum lycopersicum OX=4081 GN=A...	[more]
Q00985	2.8e-86	50.97	1-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1	[more]

BLAST of Moc11g08220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BWX3	2.2e-179	100.00	1-aminocyclopropane-1-carboxylate oxidase 5 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A0A0L1U8	1.9e-170	93.85	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G...	[more]
A0A6J1FFA0	2.5e-170	94.17	1-aminocyclopropane-1-carboxylate oxidase 5-like isoform X1 OS=Cucurbita moschat...	[more]
A0A5A7UFF3	3.2e-170	94.17	1-aminocyclopropane-1-carboxylate oxidase 5 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3B9W8	3.2e-170	94.17	1-aminocyclopropane-1-carboxylate oxidase 5 OS=Cucumis melo OX=3656 GN=LOC103487...	[more]

BLAST of Moc11g08220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G77330.1	5.7e-135	73.16	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G62380.1	3.7e-86	48.73	ACC oxidase 2	[more]
AT1G12010.1	4.9e-86	50.81	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G05010.1	2.7e-84	48.89	ethylene-forming enzyme	[more]
AT2G19590.1	8.9e-72	44.62	ACC oxidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 113..133
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 2..306
None	No IPR available	PANTHER	PTHR47991:SF120	1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE 5-LIKE	coord: 2..306
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 1..301
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 2..309 e-value: 2.7E-97 score: 327.9
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 162..255 e-value: 9.1E-27 score: 93.5
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 3..100 e-value: 5.0E-19 score: 69.2
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 151..255 score: 13.410498

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc11g08220.1	Moc11g08220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1901576	organic substance biosynthetic process
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen