Moc11g03330 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc11g03330
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Cytochrome P450
Location	chr11: 2129270 .. 2130497 (-)
RNA-Seq Expression	Moc11g03330
Synteny	Moc11g03330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTGCAAAATCTCTCTCAAAAATACGGTCCAATCGTATCGCTCTCGTTCGGTTTCCGCCCAGTGGTGGTAGTGTCCTCCCCCTCCGCCGCCCAAGAGTGCTTTACAAAAAATGACATTATTTTTGCTAGCCGCCCTCGAATGATTTCCGGCAAGATCCTAAACTACAACTACACCGCCGTAGGGGCTACCGCCTACGGCCAACACTGGCGAATTATGCGCCGCATTGCCACCACGGACCTACTATCCACTCACCGCCTCAACTTCCTTCATTAACCTTCGACAGGAGGAGGTCAAATTATGGCTCAAAAATTTGTACCGAACTACTGGCCAAATTAATGTGCGAGTAGAGATAAGGTCTAGACTGACGGAGCTGTCGTTCAACATTGCTTTGAGGATGATGACTGGGAAGCGATATTTCGGTGTGGACGTGGAAGATTCTGAGGAAGGGAGGGAGTTGAAGAAGGTGATGAAGCAAGTTCCTCTGCTAAGTGGGGCGTCGTATCCGACTGATTTTCTTTCAGTCTTAAAACTAATTGATTATCAAGGATTTAGAAAGAGGTTGTGGAAAGTGTGGGCGGGCGCTGATGGTTTCGCACAAAGTTTGATCAATGGCCGTCGCGAAAGGAAAGTCTCGTGTTCTTTTTCACCGGAGGAAGAGCGGAAGAATATGATTGATACTATGCTATCGTTACAGGACTCTGACCCTCATTATTATAAGGATCATATTATCAAGGGTCAGATTTTGGTAAGCATATTACAAAATTATAAATTTTAATTTTATAAACCCTTTGCTTTAATTTATTTAATAAGTAAAGAAAAAATATTTGTTTAATGGGTGCAGACCCTGTTGGCTGCTGGAACTGACACGACCGCGGGAACAATAGAGTGGGGGATGTCGCTACTCCTCAACCATCCAACAGCAATGAAGAAGGCATGGAGTGAGATAAATGATTACGTCGGAGAGCAGCAGTTGGTGGAGGAAGCCGATCTTGCCAACCTACCATATCTTCAGGCCATAATCAACGAAACCTATCGGATGTTTCCAGCACTCCCAATATTGGTGCCACGCGAATCGTCAGAAGACTGCACAATCGGAAGGTACGATGTTCCAAAAGGAACAATGTTGGTGGTGAATGCGTGGGCCATCCATCGGGACCCAAAGTGTGGGAGGACCCAACGAGTTTCCGGCCAGAGAGGTTTGAAAATGGGAAAATTGTGGAAGTGA

mRNA sequence

ATGCTGCAAAATCTCTCTCAAAAATACGGTCCAATCGTATCGCTCTCGTTCGGTTTCCGCCCAGTGGTGGTAGTGTCCTCCCCCTCCGCCGCCCAAGAGTGCTTTACAAAAAATGACATTATTTTTGCTAGCCGCCCTCGAATGATTTCCGGCAAGATCCTAAACTACAACTACACCGCCGAGGAGGTCAAATTATGGCTCAAAAATTTGTACCGAACTACTGGCCAAATTAATGTGCGAGTAGAGATAAGGTCTAGACTGACGGAGCTGTCGTTCAACATTGCTTTGAGGATGATGACTGGGAAGCGATATTTCGGTGTGGACGTGGAAGATTCTGAGGAAGGGAGGGAGTTGAAGAAGGTGATGAAGCAAGTTCCTCTGCTAAGTGGGGCGTCGTATCCGACTGATTTTCTTTCAGTCTTAAAACTAATTGATTATCAAGGATTTAGAAAGAGGTTGTGGAAAGTGTGGGCGGGCGCTGATGGTTTCGCACAAAGTTTGATCAATGGCCGTCGCGAAAGGAAAGTCTCGTGTTCTTTTTCACCGGAGGAAGAGCGGAAGAATATGATTGATACTATGCTATCGTTACAGGACTCTGACCCTCATTATTATAAGGATCATATTATCAAGGGTCAGATTTTGACCCTGTTGGCTGCTGGAACTGACACGACCGCGGGAACAATAGAGTGGGGGATGTCGCTACTCCTCAACCATCCAACAGCAATGAAGAAGGCATGGAGTGAGATAAATGATTACGTCGGAGAGCAGCAGTTGGTGGAGGAAGCCGATCTTGCCAACCTACCATATCTTCAGGCCATAATCAACGAAACCTATCGGATGTTTCCAGCACTCCCAATATTGGTGCCACGCGAATCGTCAGAAGACTGCACAATCGGAAGGTACGATGTTCCAAAAGGAACAATGTTGGTGGTGAATGCGTGGGCCATCCATCGGGACCCAAAGTGTGGGAGGACCCAACGAGTTTCCGGCCAGAGAGGTTTGAAAATGGGAAAATTGTGGAAGTGA

Coding sequence (CDS)

ATGCTGCAAAATCTCTCTCAAAAATACGGTCCAATCGTATCGCTCTCGTTCGGTTTCCGCCCAGTGGTGGTAGTGTCCTCCCCCTCCGCCGCCCAAGAGTGCTTTACAAAAAATGACATTATTTTTGCTAGCCGCCCTCGAATGATTTCCGGCAAGATCCTAAACTACAACTACACCGCCGAGGAGGTCAAATTATGGCTCAAAAATTTGTACCGAACTACTGGCCAAATTAATGTGCGAGTAGAGATAAGGTCTAGACTGACGGAGCTGTCGTTCAACATTGCTTTGAGGATGATGACTGGGAAGCGATATTTCGGTGTGGACGTGGAAGATTCTGAGGAAGGGAGGGAGTTGAAGAAGGTGATGAAGCAAGTTCCTCTGCTAAGTGGGGCGTCGTATCCGACTGATTTTCTTTCAGTCTTAAAACTAATTGATTATCAAGGATTTAGAAAGAGGTTGTGGAAAGTGTGGGCGGGCGCTGATGGTTTCGCACAAAGTTTGATCAATGGCCGTCGCGAAAGGAAAGTCTCGTGTTCTTTTTCACCGGAGGAAGAGCGGAAGAATATGATTGATACTATGCTATCGTTACAGGACTCTGACCCTCATTATTATAAGGATCATATTATCAAGGGTCAGATTTTGACCCTGTTGGCTGCTGGAACTGACACGACCGCGGGAACAATAGAGTGGGGGATGTCGCTACTCCTCAACCATCCAACAGCAATGAAGAAGGCATGGAGTGAGATAAATGATTACGTCGGAGAGCAGCAGTTGGTGGAGGAAGCCGATCTTGCCAACCTACCATATCTTCAGGCCATAATCAACGAAACCTATCGGATGTTTCCAGCACTCCCAATATTGGTGCCACGCGAATCGTCAGAAGACTGCACAATCGGAAGGTACGATGTTCCAAAAGGAACAATGTTGGTGGTGAATGCGTGGGCCATCCATCGGGACCCAAAGTGTGGGAGGACCCAACGAGTTTCCGGCCAGAGAGGTTTGAAAATGGGAAAATTGTGGAAGTGA

Protein sequence

MLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTAEEVKLWLKNLYRTTGQINVRVEIRSRLTELSFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLIDYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADLANLPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPKCGRTQRVSGQRGLKMGKLWK

Homology

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BLAST of Moc11g03330 vs. NCBI nr
Match: XP_022138249.1 (isoflavone 2'-hydroxylase-like [Momordica charantia])

HSP 1 Score: 573.9 bits (1478), Expect = 9.0e-160
Identity = 288/356 (80.90%), Postives = 306/356 (85.96%), Query Frame = 0


Query: 1   MLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTA 60
           MLQNLS+KYGPI+SLS GFR VVVVSSPSAAQECFTKNDI+FA+RPRMISGKILNYNYT+
Sbjct: 59  MLQNLSKKYGPILSLSLGFRSVVVVSSPSAAQECFTKNDIVFANRPRMISGKILNYNYTS 118

Query: 61  -----------------------------------EEVKLWLKNLYRTTGQINVRVEIRS 120
                                              +EVKLWLKNLY+TTGQ NV+VEIR+
Sbjct: 119 VGATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQQEVKLWLKNLYQTTGQNNVQVEIRT 178

Query: 121 RLTELSFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLID 180
           +LTELSFNIALRMMTGKRYFGVDVEDSEEGR+LKKVMK+V LLSGASYP DFLSVLKLID
Sbjct: 179 KLTELSFNIALRMMTGKRYFGVDVEDSEEGRKLKKVMKEVSLLSGASYPADFLSVLKLID 238

Query: 181 YQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYK 240
           YQGFRKRLWKVWAGADGFAQSLI+GRRERK SCSFSPEE RKNMIDTMLSLQDSDP YYK
Sbjct: 239 YQGFRKRLWKVWAGADGFAQSLIDGRRERKASCSFSPEESRKNMIDTMLSLQDSDPGYYK 298

Query: 241 DHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADLA 300
           DHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNH T MKK WSEIN+YVGEQ+LVEEADL 
Sbjct: 299 DHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHRTIMKKVWSEINEYVGEQRLVEEADLT 358

Query: 301 NLPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPK 322
           NLP+LQAIINET R+FPALPILVPRESSEDCTIG YD+PKGTMLVVN WAIHRDPK
Sbjct: 359 NLPFLQAIINETNRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNVWAIHRDPK 414

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BLAST of Moc11g03330 vs. NCBI nr
Match: XP_022136931.1 (isoflavone 2'-hydroxylase-like [Momordica charantia])

HSP 1 Score: 564.3 bits (1453), Expect = 7.1e-157
Identity = 286/356 (80.34%), Postives = 298/356 (83.71%), Query Frame = 0


Query: 1   MLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTA 60
           MLQNLSQ+YGPI+SLS GFR VVVVSSP AAQECFTKNDI+FA+RPRMISGKILNYNYT 
Sbjct: 59  MLQNLSQRYGPILSLSLGFRSVVVVSSPIAAQECFTKNDIVFANRPRMISGKILNYNYTT 118

Query: 61  -----------------------------------EEVKLWLKNLYRTTGQINVRVEIRS 120
                                              EEVKLWLKNLYR TGQ NVRVEIRS
Sbjct: 119 VGATAYGQHWRIMRRIATTELLSTHRLISFINLRQEEVKLWLKNLYRITGQNNVRVEIRS 178

Query: 121 RLTELSFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLID 180
           RLTELSFNI LRMMTGKRYFGV+V+DSEE RELKKVMK+V LLSG SYP DFLSVL+LID
Sbjct: 179 RLTELSFNIVLRMMTGKRYFGVEVKDSEEERELKKVMKEVSLLSGMSYPADFLSVLRLID 238

Query: 181 YQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYK 240
           YQGFRKRLWKVWAGADGF Q LI+GRRE K SCSFS EE  KNMIDTMLSLQDSDP YYK
Sbjct: 239 YQGFRKRLWKVWAGADGFVQXLIDGRREMKASCSFSLEENWKNMIDTMLSLQDSDPDYYK 298

Query: 241 DHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADLA 300
           DHIIKGQILTLL AGTDTTAGTIEWGMSLLLNHPT MKKAWSEINDYVGEQ+LVEEADLA
Sbjct: 299 DHIIKGQILTLLVAGTDTTAGTIEWGMSLLLNHPTIMKKAWSEINDYVGEQRLVEEADLA 358

Query: 301 NLPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPK 322
           NLPYLQAIINETYR+FPALPILVPRESSEDCTIG YDVPKGTMLVVN WAIHRDP+
Sbjct: 359 NLPYLQAIINETYRLFPALPILVPRESSEDCTIGGYDVPKGTMLVVNMWAIHRDPQ 414

[Close]

BLAST of Moc11g03330 vs. NCBI nr
Match: XP_022982228.1 (isoflavone 3'-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 486.9 bits (1252), Expect = 1.4e-133
Identity = 251/357 (70.31%), Postives = 282/357 (78.99%), Query Frame = 0


Query: 1   MLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTA 60
           MLQ+LSQKYGPI+SLS GFRPVVVVSSP+AAQECF+KNDIIFASRPRMISGKILNY+YTA
Sbjct: 63  MLQDLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTA 122

Query: 61  -----------------------------------EEVKLWLKNLYRTTGQINVRVEIRS 120
                                              +EVKLWLK+LYR TG+ NVRVEIRS
Sbjct: 123 VGATAYGQHWRIMRRIATTELLSTHRLNALINIRQQEVKLWLKSLYRITGKNNVRVEIRS 182

Query: 121 RLTELSFNIALRMMTGKRYFGVD-VEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLI 180
           +LTELSFNIAL +M GKRYFG + V+DSEE RELK  MKQVPLLSGASYP DFLSVLKL+
Sbjct: 183 KLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLV 242

Query: 181 DYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYY 240
           DYQGFRK+L KV    D FAQSLI+ RR +K     +   ERK MID++LS+Q  +P YY
Sbjct: 243 DYQGFRKKLAKVRVDGDAFAQSLIDERRNKK-----ADHNERKTMIDSLLSMQQMEPEYY 302

Query: 241 KDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADL 300
           KDHIIKGQ LTLLAAGTDTTAGT+EW +SLLLNHPTAMKKAW+EIN++VG+++LVEE DL
Sbjct: 303 KDHIIKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDL 362

Query: 301 ANLPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPK 322
             L YLQ IINETYR+FPALPILVPRESSEDCTIG +DVP GTMLVVNAWAIHRDPK
Sbjct: 363 NQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPIGTMLVVNAWAIHRDPK 414

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BLAST of Moc11g03330 vs. NCBI nr
Match: KAG7037779.1 (Cytochrome P450 81E8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 486.9 bits (1252), Expect = 1.4e-133
Identity = 251/357 (70.31%), Postives = 283/357 (79.27%), Query Frame = 0


Query: 1   MLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTA 60
           MLQ+LSQKYGPI+SLS GFRPVVVVSSP+AAQECF+KNDIIFASRPRMISGKILNY+YTA
Sbjct: 76  MLQDLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTA 135

Query: 61  -----------------------------------EEVKLWLKNLYRTTGQINVRVEIRS 120
                                              +EVKLWLK+LYR +G+ NVRVEIRS
Sbjct: 136 VGATAYGQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISGKNNVRVEIRS 195

Query: 121 RLTELSFNIALRMMTGKRYFGVD-VEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLI 180
           +LTELSFNIAL +M GKRYFG + V+DSEE RELK  MKQVPLLSGASYP DFLSVLKL+
Sbjct: 196 KLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLV 255

Query: 181 DYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYY 240
           DYQGFRKRL KV    D FAQSLI+ RR +K     + + ERK MID++LS+Q  +P YY
Sbjct: 256 DYQGFRKRLAKVRVDGDAFAQSLIDERRNKK-----ADDNERKTMIDSLLSMQQMEPEYY 315

Query: 241 KDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADL 300
           KD IIKGQ LTLLAAGTDTTAGT+EW +SLLLNHPTAMKKAW+EIN++VG+++LVEE DL
Sbjct: 316 KDDIIKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDL 375

Query: 301 ANLPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPK 322
             L YLQ IINETYR+FPALPILVPRESSEDCTIG +DVPKGTMLVVNAWAIHRDPK
Sbjct: 376 NQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPK 427

[Close]

BLAST of Moc11g03330 vs. NCBI nr
Match: XP_022940149.1 (cytochrome P450 81E8-like [Cucurbita moschata])

HSP 1 Score: 486.9 bits (1252), Expect = 1.4e-133
Identity = 251/357 (70.31%), Postives = 283/357 (79.27%), Query Frame = 0


Query: 1   MLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTA 60
           MLQ+LSQKYGPI+SLS GFRPVVVVSSP+AAQECF+KNDIIFASRPRMISGKILNY+YTA
Sbjct: 62  MLQDLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTA 121

Query: 61  -----------------------------------EEVKLWLKNLYRTTGQINVRVEIRS 120
                                              +EVKLWLK+LYR +G+ NVRVEIRS
Sbjct: 122 VGATAYGQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISGKNNVRVEIRS 181

Query: 121 RLTELSFNIALRMMTGKRYFGVD-VEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLI 180
           +LTELSFNIAL +M GKRYFG + V+DSEE RELK  MKQVPLLSGASYP DFLSVLKL+
Sbjct: 182 KLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLV 241

Query: 181 DYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYY 240
           DYQGFRKRL KV    D FAQSLI+ RR +K     + + ERK MID++LS+Q  +P YY
Sbjct: 242 DYQGFRKRLAKVRVDGDAFAQSLIDERRNKK-----ADDNERKTMIDSLLSMQQMEPEYY 301

Query: 241 KDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADL 300
           KD IIKGQ LTLLAAGTDTTAGT+EW +SLLLNHPTAMKKAW+EIN++VG+++LVEE DL
Sbjct: 302 KDDIIKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDL 361

Query: 301 ANLPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPK 322
             L YLQ IINETYR+FPALPILVPRESSEDCTIG +DVPKGTMLVVNAWAIHRDPK
Sbjct: 362 NQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPK 413

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BLAST of Moc11g03330 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 2.0e-85
Identity = 170/355 (47.89%), Postives = 227/355 (63.94%), Query Frame = 0


Query: 2   LQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTA- 61
           L +LS+KYG + SL  G R V+VVSSP+AA+ECFTKNDI+FA+RP  I GK + YNYT  
Sbjct: 57  LYDLSKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTM 116

Query: 62  ----------------------------------EEVKLWLKNLYRTTGQINVRVEIRSR 121
                                             +EVK  L +LY+++GQ   +VE++S+
Sbjct: 117 VGSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSK 176

Query: 122 LTELSFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLIDY 181
           L+ELSFN+ +RM+ GKRYFG DV DS+E +  + ++ +V   +GAS P DF+  L+ ID+
Sbjct: 177 LSELSFNVTMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDF 236

Query: 182 QGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKD 241
           + + K++ K+    D F Q LI+  R  K             MID +LSLQ+S P YY D
Sbjct: 237 KNYEKKVSKISQEMDAFLQRLIHESRINK---------NNVTMIDHLLSLQESQPEYYTD 296

Query: 242 HIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADLAN 301
            IIKG I+ LL AGTDT+A T+EW MSLLLNHP  ++KA +EI   VG  +L+EE DL  
Sbjct: 297 QIIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPK 356

Query: 302 LPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPK 322
           L YL  II+ET+R+ PA P+LVP ESS+DC +  YDVPKGT+L+VNAWAIHRDP+
Sbjct: 357 LTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPE 401

[Close]

BLAST of Moc11g03330 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.7e-76
Identity = 158/358 (44.13%), Postives = 215/358 (60.06%), Query Frame = 0


Query: 5   LSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYT----A 64
           LSQ YG I SL FG R VVVVSSPS A ECFTKNDII A+RPR ++GK + YNYT    A
Sbjct: 57  LSQTYGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSA 116

Query: 65  EEVKLWLKNLYRTT-------GQIN----------------------------VRVEIRS 124
                W +NL R T        ++N                             +VE+R 
Sbjct: 117 SYGDHW-RNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRP 176

Query: 125 RLTELSFNIALRMMTGKRYFG--VDVEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKL 184
           RLTE++FN  +RM++GKRY+G   DV D EE ++ ++++ ++  L GA+   DFL +L++
Sbjct: 177 RLTEMTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRV 236

Query: 185 IDYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHY 244
           +D     KR  ++   ++ F + LI   R   +        +   MID +L L +S P Y
Sbjct: 237 VDLDNLEKRCKRIAKRSNAFLEGLIEEHRRGNI------HSDGGTMIDHLLKLSESQPEY 296

Query: 245 YKDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEAD 304
           Y DH+IKG I  +L AGTDT+A TIEW MS LLNHP  +KKA  E++  +G+ +LV+E D
Sbjct: 297 YSDHLIKGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQD 356

Query: 305 LANLPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPK 322
           L+ LPYLQ II+ET R+ P  P+L+P  SSEDCTIG ++VPK T+++ N W IHRDPK
Sbjct: 357 LSKLPYLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPK 407

[Close]

BLAST of Moc11g03330 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 278.1 bits (710), Expect = 1.3e-73
Identity = 148/357 (41.46%), Postives = 215/357 (60.22%), Query Frame = 0


Query: 3   QNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYT--- 62
           + LS+KYG + SL FG R VVVVSS S  Q+CFTKND++ A+RPR +SGK + YNYT   
Sbjct: 57  KGLSEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLG 116

Query: 63  -AEEVKLWLKNLYRTTG-------QIN-------------------------VRVEIRSR 122
                + W +NL R T        +IN                           +E+ SR
Sbjct: 117 STSYGEHW-RNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSR 176

Query: 123 LTELSFNIALRMMTGKRYFG--VDVEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLI 182
           L +++FN  +RM++GKRY+G   D  D +E  + + ++ ++  LSGA+  TDF+ +L+ +
Sbjct: 177 LYDMTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFL 236

Query: 183 DYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYY 242
           D++   KRL  +    D F + LI   R +K        E    MID +L+LQDS P YY
Sbjct: 237 DFENLEKRLKDISGKTDAFLRGLIEEHRTKK--------ERANTMIDHLLNLQDSQPEYY 296

Query: 243 KDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADL 302
            D IIKG  L +L AGTD++A T+EW MS LLNHP  +KK   E++ +VG+ +LV+E+DL
Sbjct: 297 TDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDL 356

Query: 303 ANLPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPK 322
             L YL+ +INET R++   P+L+P  +S++C IG Y VP+ T++++NAWAIHRDP+
Sbjct: 357 PKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPE 404

[Close]

BLAST of Moc11g03330 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 3.9e-73
Identity = 151/353 (42.78%), Postives = 207/353 (58.64%), Query Frame = 0


Query: 5   LSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTA---- 64
           LSQKYG I SL FG R VVVVSS + AQECFTKNDI+ A+RP  ++GK + YN T     
Sbjct: 59  LSQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQS 118

Query: 65  -------------------------------EEVKLWLKNLYRTTGQINVRVEIRSRLTE 124
                                          +E+   ++ L + +      VE+R   +E
Sbjct: 119 PYGDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSE 178

Query: 125 LSFNIALRMMTGKRYFG--VDVEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLIDYQ 184
           ++FN  +RM++GKRY+G   DV D EE R  + ++K+V  L GA+   DFL  L+  D+ 
Sbjct: 179 MTFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFD 238

Query: 185 GFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKDH 244
           G  KRL K+    D F Q LI+  R  K             MID +L+ Q S P YY D 
Sbjct: 239 GLEKRLKKISKRTDAFLQGLIDEHRFGK--------RNSNTMIDHLLTQQQSQPEYYTDQ 298

Query: 245 IIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADLANL 304
           IIKG ++ +L AGTDT++ TIEW MS LLNHP  MKKA +E++ ++G  + V+E D++ L
Sbjct: 299 IIKGLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKL 358

Query: 305 PYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDP 321
           PYLQ+I+ ET R+  A P+LVP  SSED ++G Y++P+ T+L+VNAW IHRDP
Sbjct: 359 PYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDP 403

[Close]

BLAST of Moc11g03330 vs. ExPASy Swiss-Prot
Match: Q0WTF4 (Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.1e-70
Identity = 141/357 (39.50%), Postives = 213/357 (59.66%), Query Frame = 0


Query: 1   MLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFT-KNDIIFASRPRMISGKILNYNYT 60
           +   LS+ +GPI SL FG R  VV+SS S A +CFT +NDII ++RP  ++ K + YNYT
Sbjct: 54  LFHRLSKTHGPIFSLQFGSRRAVVISSSSLATQCFTGQNDIILSNRPCFLTAKYVAYNYT 113

Query: 61  A-----------------------------------EEVKLWLKNLYRTTGQINVRVEIR 120
                                               +E+   L  L R    +N  +E+ 
Sbjct: 114 TVGTAPYGDHWRNLRRICSLEILSSNRLTNFLHIRKDEIHRMLTRLSR---DVNKEIELE 173

Query: 121 SRLTELSFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLI 180
             L++L+FN  +RM+TGKRY+G +V + EE    KK++  +   SGA +P D+L  +K+ 
Sbjct: 174 PLLSDLTFNNIVRMVTGKRYYGDEVHNEEEANVFKKLVADINDCSGARHPGDYLPFMKMF 233

Query: 181 DYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYY 240
               F K++  +    D   Q L+   +          +++   M++ +LSLQ ++P YY
Sbjct: 234 G-GSFEKKVKALAEAMDEILQRLLEECKR---------DKDGNTMVNHLLSLQQNEPEYY 293

Query: 241 KDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADL 300
            D  IKG +L ++ AGTDT+A T+EW MS LLNHP A++KA  EI++ +G+++L++E D+
Sbjct: 294 TDVTIKGLMLGMMIAGTDTSAVTLEWAMSSLLNHPEALEKAKLEIDEKIGQERLIDEPDI 353

Query: 301 ANLPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPK 322
           ANLPYLQ I++ET+R++PA P+LVPR  +ED  +G YDVP+GTM++VNAWAIHRDP+
Sbjct: 354 ANLPYLQNIVSETFRLYPAAPLLVPRSPTEDIKVGGYDVPRGTMVMVNAWAIHRDPE 397

[Close]

BLAST of Moc11g03330 vs. ExPASy TrEMBL
Match: A0A6J1C9L7 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111009467 PE=3 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 4.3e-160
Identity = 288/356 (80.90%), Postives = 306/356 (85.96%), Query Frame = 0


Query: 1   MLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTA 60
           MLQNLS+KYGPI+SLS GFR VVVVSSPSAAQECFTKNDI+FA+RPRMISGKILNYNYT+
Sbjct: 59  MLQNLSKKYGPILSLSLGFRSVVVVSSPSAAQECFTKNDIVFANRPRMISGKILNYNYTS 118

Query: 61  -----------------------------------EEVKLWLKNLYRTTGQINVRVEIRS 120
                                              +EVKLWLKNLY+TTGQ NV+VEIR+
Sbjct: 119 VGATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQQEVKLWLKNLYQTTGQNNVQVEIRT 178

Query: 121 RLTELSFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLID 180
           +LTELSFNIALRMMTGKRYFGVDVEDSEEGR+LKKVMK+V LLSGASYP DFLSVLKLID
Sbjct: 179 KLTELSFNIALRMMTGKRYFGVDVEDSEEGRKLKKVMKEVSLLSGASYPADFLSVLKLID 238

Query: 181 YQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYK 240
           YQGFRKRLWKVWAGADGFAQSLI+GRRERK SCSFSPEE RKNMIDTMLSLQDSDP YYK
Sbjct: 239 YQGFRKRLWKVWAGADGFAQSLIDGRRERKASCSFSPEESRKNMIDTMLSLQDSDPGYYK 298

Query: 241 DHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADLA 300
           DHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNH T MKK WSEIN+YVGEQ+LVEEADL 
Sbjct: 299 DHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHRTIMKKVWSEINEYVGEQRLVEEADLT 358

Query: 301 NLPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPK 322
           NLP+LQAIINET R+FPALPILVPRESSEDCTIG YD+PKGTMLVVN WAIHRDPK
Sbjct: 359 NLPFLQAIINETNRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNVWAIHRDPK 414

[Close]

BLAST of Moc11g03330 vs. ExPASy TrEMBL
Match: A0A6J1C4W6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111008503 PE=3 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.4e-157
Identity = 286/356 (80.34%), Postives = 298/356 (83.71%), Query Frame = 0


Query: 1   MLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTA 60
           MLQNLSQ+YGPI+SLS GFR VVVVSSP AAQECFTKNDI+FA+RPRMISGKILNYNYT 
Sbjct: 59  MLQNLSQRYGPILSLSLGFRSVVVVSSPIAAQECFTKNDIVFANRPRMISGKILNYNYTT 118

Query: 61  -----------------------------------EEVKLWLKNLYRTTGQINVRVEIRS 120
                                              EEVKLWLKNLYR TGQ NVRVEIRS
Sbjct: 119 VGATAYGQHWRIMRRIATTELLSTHRLISFINLRQEEVKLWLKNLYRITGQNNVRVEIRS 178

Query: 121 RLTELSFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLID 180
           RLTELSFNI LRMMTGKRYFGV+V+DSEE RELKKVMK+V LLSG SYP DFLSVL+LID
Sbjct: 179 RLTELSFNIVLRMMTGKRYFGVEVKDSEEERELKKVMKEVSLLSGMSYPADFLSVLRLID 238

Query: 181 YQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYK 240
           YQGFRKRLWKVWAGADGF Q LI+GRRE K SCSFS EE  KNMIDTMLSLQDSDP YYK
Sbjct: 239 YQGFRKRLWKVWAGADGFVQXLIDGRREMKASCSFSLEENWKNMIDTMLSLQDSDPDYYK 298

Query: 241 DHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADLA 300
           DHIIKGQILTLL AGTDTTAGTIEWGMSLLLNHPT MKKAWSEINDYVGEQ+LVEEADLA
Sbjct: 299 DHIIKGQILTLLVAGTDTTAGTIEWGMSLLLNHPTIMKKAWSEINDYVGEQRLVEEADLA 358

Query: 301 NLPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPK 322
           NLPYLQAIINETYR+FPALPILVPRESSEDCTIG YDVPKGTMLVVN WAIHRDP+
Sbjct: 359 NLPYLQAIINETYRLFPALPILVPRESSEDCTIGGYDVPKGTMLVVNMWAIHRDPQ 414

[Close]

BLAST of Moc11g03330 vs. ExPASy TrEMBL
Match: A0A6J1J4B3 (isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111481120 PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 7.0e-134
Identity = 251/357 (70.31%), Postives = 282/357 (78.99%), Query Frame = 0


Query: 1   MLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTA 60
           MLQ+LSQKYGPI+SLS GFRPVVVVSSP+AAQECF+KNDIIFASRPRMISGKILNY+YTA
Sbjct: 63  MLQDLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTA 122

Query: 61  -----------------------------------EEVKLWLKNLYRTTGQINVRVEIRS 120
                                              +EVKLWLK+LYR TG+ NVRVEIRS
Sbjct: 123 VGATAYGQHWRIMRRIATTELLSTHRLNALINIRQQEVKLWLKSLYRITGKNNVRVEIRS 182

Query: 121 RLTELSFNIALRMMTGKRYFGVD-VEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLI 180
           +LTELSFNIAL +M GKRYFG + V+DSEE RELK  MKQVPLLSGASYP DFLSVLKL+
Sbjct: 183 KLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLV 242

Query: 181 DYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYY 240
           DYQGFRK+L KV    D FAQSLI+ RR +K     +   ERK MID++LS+Q  +P YY
Sbjct: 243 DYQGFRKKLAKVRVDGDAFAQSLIDERRNKK-----ADHNERKTMIDSLLSMQQMEPEYY 302

Query: 241 KDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADL 300
           KDHIIKGQ LTLLAAGTDTTAGT+EW +SLLLNHPTAMKKAW+EIN++VG+++LVEE DL
Sbjct: 303 KDHIIKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDL 362

Query: 301 ANLPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPK 322
             L YLQ IINETYR+FPALPILVPRESSEDCTIG +DVP GTMLVVNAWAIHRDPK
Sbjct: 363 NQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPIGTMLVVNAWAIHRDPK 414

[Close]

BLAST of Moc11g03330 vs. ExPASy TrEMBL
Match: A0A6J1FJ78 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111445861 PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 7.0e-134
Identity = 251/357 (70.31%), Postives = 283/357 (79.27%), Query Frame = 0


Query: 1   MLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTA 60
           MLQ+LSQKYGPI+SLS GFRPVVVVSSP+AAQECF+KNDIIFASRPRMISGKILNY+YTA
Sbjct: 62  MLQDLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTA 121

Query: 61  -----------------------------------EEVKLWLKNLYRTTGQINVRVEIRS 120
                                              +EVKLWLK+LYR +G+ NVRVEIRS
Sbjct: 122 VGATAYGQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISGKNNVRVEIRS 181

Query: 121 RLTELSFNIALRMMTGKRYFGVD-VEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLI 180
           +LTELSFNIAL +M GKRYFG + V+DSEE RELK  MKQVPLLSGASYP DFLSVLKL+
Sbjct: 182 KLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLV 241

Query: 181 DYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYY 240
           DYQGFRKRL KV    D FAQSLI+ RR +K     + + ERK MID++LS+Q  +P YY
Sbjct: 242 DYQGFRKRLAKVRVDGDAFAQSLIDERRNKK-----ADDNERKTMIDSLLSMQQMEPEYY 301

Query: 241 KDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADL 300
           KD IIKGQ LTLLAAGTDTTAGT+EW +SLLLNHPTAMKKAW+EIN++VG+++LVEE DL
Sbjct: 302 KDDIIKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDL 361

Query: 301 ANLPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPK 322
             L YLQ IINETYR+FPALPILVPRESSEDCTIG +DVPKGTMLVVNAWAIHRDPK
Sbjct: 362 NQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPK 413

[Close]

BLAST of Moc11g03330 vs. ExPASy TrEMBL
Match: A0A0A0L2I1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641760 PE=3 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 3.3e-123
Identity = 238/386 (61.66%), Postives = 280/386 (72.54%), Query Frame = 0


Query: 1   MLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTA 60
           MLQ+ S+KYGP+ SL+ GFRP+VVVSSPSA QECFTKNDI+FASRPRM+SGKILNY+YTA
Sbjct: 68  MLQDFSKKYGPVFSLTLGFRPLVVVSSPSAVQECFTKNDIVFASRPRMLSGKILNYDYTA 127

Query: 61  -----------------------------------EEVKLWLKNLYRTTGQINV----RV 120
                                              EEVKLW+K LY +  +  V    RV
Sbjct: 128 VGATAYGQHWRIMRRIATTELLSTKRLNDLSKIREEEVKLWVKRLYGSVTRAEVNKGGRV 187

Query: 121 EIRSRLTELSFNIALRMMTGKRYFGVDVE--DSEEGRELKKVMKQVPLLSGASYPTDFLS 180
            +RS+LTELSFNIAL +M G+RYFG ++E  +SEE +ELK+ MKQVPLLSGASYP DFLS
Sbjct: 188 GLRSKLTELSFNIALMLMAGRRYFGDEMEAGESEEAKELKETMKQVPLLSGASYPADFLS 247

Query: 181 VLKLIDYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDS 240
           VLKLID+QGFRKRL KV    D FAQSL++ RR+ K S       ERK MID+ML LQ+S
Sbjct: 248 VLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRKLKGSWC-----ERKTMIDSMLCLQES 307

Query: 241 DPHYYKDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLV 300
           DP YYKD IIKGQ LTLLAAGTDTTA T+EW MSLLLNHPT ++KAW+EIN  VG+Q+L+
Sbjct: 308 DPEYYKDDIIKGQFLTLLAAGTDTTASTLEWAMSLLLNHPTVLEKAWTEINQVVGDQRLI 367

Query: 301 EEADLANLPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHR- 340
            EAD   L YLQAIINETYR+FPALPILVPRESSEDCTIG +D+PKGTMLVVNAWA+HR 
Sbjct: 368 READTNKLHYLQAIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWAVHRD 427

[Close]

BLAST of Moc11g03330 vs. TAIR 10
Match: AT4G37400.1 (cytochrome P450, family 81, subfamily F, polypeptide 3 )

HSP 1 Score: 266.9 bits (681), Expect = 2.2e-71
Identity = 141/357 (39.50%), Postives = 213/357 (59.66%), Query Frame = 0


Query: 1   MLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFT-KNDIIFASRPRMISGKILNYNYT 60
           +   LS+ +GPI SL FG R  VV+SS S A +CFT +NDII ++RP  ++ K + YNYT
Sbjct: 54  LFHRLSKTHGPIFSLQFGSRRAVVISSSSLATQCFTGQNDIILSNRPCFLTAKYVAYNYT 113

Query: 61  A-----------------------------------EEVKLWLKNLYRTTGQINVRVEIR 120
                                               +E+   L  L R    +N  +E+ 
Sbjct: 114 TVGTAPYGDHWRNLRRICSLEILSSNRLTNFLHIRKDEIHRMLTRLSR---DVNKEIELE 173

Query: 121 SRLTELSFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLI 180
             L++L+FN  +RM+TGKRY+G +V + EE    KK++  +   SGA +P D+L  +K+ 
Sbjct: 174 PLLSDLTFNNIVRMVTGKRYYGDEVHNEEEANVFKKLVADINDCSGARHPGDYLPFMKMF 233

Query: 181 DYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYY 240
               F K++  +    D   Q L+   +          +++   M++ +LSLQ ++P YY
Sbjct: 234 G-GSFEKKVKALAEAMDEILQRLLEECKR---------DKDGNTMVNHLLSLQQNEPEYY 293

Query: 241 KDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADL 300
            D  IKG +L ++ AGTDT+A T+EW MS LLNHP A++KA  EI++ +G+++L++E D+
Sbjct: 294 TDVTIKGLMLGMMIAGTDTSAVTLEWAMSSLLNHPEALEKAKLEIDEKIGQERLIDEPDI 353

Query: 301 ANLPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPK 322
           ANLPYLQ I++ET+R++PA P+LVPR  +ED  +G YDVP+GTM++VNAWAIHRDP+
Sbjct: 354 ANLPYLQNIVSETFRLYPAAPLLVPRSPTEDIKVGGYDVPRGTMVMVNAWAIHRDPE 397

[Close]

BLAST of Moc11g03330 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 265.0 bits (676), Expect = 8.3e-71
Identity = 145/355 (40.85%), Postives = 212/355 (59.72%), Query Frame = 0


Query: 4   NLSQKYG--PIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYT-- 63
           +LS+  G  P+  L  G R V V+SS S A+ECFTKND++ A+RP+    K L YN T  
Sbjct: 58  SLSKSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYL 117

Query: 64  ---------------------------------AEEVKLWLKNLYRTTGQINVRVEIRSR 123
                                             +E++  + +L R +    V VE+++ 
Sbjct: 118 LSASYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTL 177

Query: 124 LTELSFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLIDY 183
           LT L+ N  +RM+ GKRYFG   ED+++ + +K ++ +    +GA  P D+LS+L+ +  
Sbjct: 178 LTNLASNTTIRMLAGKRYFG---EDNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV-- 237

Query: 184 QGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKD 243
             + KR+  +    D F Q L++ +R  K        E+ + MID +L+LQD  P YY D
Sbjct: 238 SSYEKRIKNLGNRFDTFLQKLVDEKRAEK--------EKGETMIDHLLALQDIQPDYYTD 297

Query: 244 HIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADLAN 303
            IIKG ILTL+ AGTDT++ T+EW MS LLNHP  +KKA  EI++ VG  +LV+E+D+ N
Sbjct: 298 VIIKGIILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVN 357

Query: 304 LPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPK 322
           L YLQ+I+ ET RM+PA+P+L+P  SSEDC +G YD+P GTM++ NAWA+HRDP+
Sbjct: 358 LSYLQSIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPE 399

[Close]

BLAST of Moc11g03330 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 263.8 bits (673), Expect = 1.9e-70
Identity = 139/346 (40.17%), Postives = 207/346 (59.83%), Query Frame = 0


Query: 11  PIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTA---------- 70
           PI SL  G R V V SS S A+ECFTKND++ A+RP  I  K + Y+YT           
Sbjct: 66  PIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHW 125

Query: 71  -------------------------EEVKLWLKNLYRTTGQINVRVEIRSRLTELSFNIA 130
                                    +E++  +  L R   Q  V+V+++S L++L+FN  
Sbjct: 126 RNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNI 185

Query: 131 LRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLIDYQGFRKRLWK 190
           LRM+ GKRY+G  VED  E + +++++  V   +GA    D+L VL+L+    +  R+ K
Sbjct: 186 LRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRVKK 245

Query: 191 VWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKDHIIKGQILT 250
           +    D F Q L++ +RE K        E+   MID +L+LQ+S P Y+ D IIKG +L 
Sbjct: 246 LAGRLDEFLQGLVDEKREAK--------EKGNTMIDHLLTLQESQPDYFTDRIIKGNMLA 305

Query: 251 LLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADLANLPYLQAIIN 310
           L+ AGTDT+A T+EW +S +LNHP  + KA  EI+  +G  +L++E+D++NLPYLQ I++
Sbjct: 306 LILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVS 365

Query: 311 ETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPK 322
           ET R++PA P+L+P  +SEDC +  YD+P+GT+L+ N WAIHRDP+
Sbjct: 366 ETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQ 401

[Close]

BLAST of Moc11g03330 vs. TAIR 10
Match: AT4G37430.1 (cytochrome P450, family 91, subfamily A, polypeptide 2 )

HSP 1 Score: 261.5 bits (667), Expect = 9.2e-70
Identity = 139/362 (38.40%), Postives = 214/362 (59.12%), Query Frame = 0


Query: 1   MLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFT-KNDIIFASRPRMISGKILNYNYT 60
           +LQ  S +YGPI SL FG R VVV++SPS AQE FT +NDI+ +SRP  ++ K + YN+T
Sbjct: 51  LLQRYSNQYGPIFSLRFGSRRVVVITSPSLAQESFTGQNDIVLSSRPLQLTAKYVAYNHT 110

Query: 61  A-----------------------------------EEVKLWLKNLYRTTGQIN-----V 120
                                               +E+   L  L R T   N      
Sbjct: 111 TVGTAPYGDHWRNLRRICSQEILSSHRLINFQHIRKDEILRMLTRLSRYTQTSNESNDFT 170

Query: 121 RVEIRSRLTELSFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPTDFLS 180
            +E+   L++L+FN  +RM+TGKRY+G DV + EE    KK++  + + SGA++  D+L 
Sbjct: 171 HIELEPLLSDLTFNNIVRMVTGKRYYGDDVNNKEEAELFKKLVYDIAMYSGANHSADYLP 230

Query: 181 VLKLIDYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDS 240
           +LKL   + F K +  +    D   Q L++  R          ++E   M++ ++SLQ  
Sbjct: 231 ILKLFGNK-FEKEVKAIGKSMDDILQRLLDECRR---------DKEGNTMVNHLISLQQQ 290

Query: 241 DPHYYKDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLV 300
            P YY D IIKG +++++ AGT+T+A T+EW M+ LL +P  ++KA SEI++ +G+ +L+
Sbjct: 291 QPEYYTDVIIKGLMMSMMLAGTETSAVTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLI 350

Query: 301 EEADLANLPYLQAIINETYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRD 322
           +E+D+A LPYLQ +++ET+R+FP  P L+PR  ++D  IG YDVP+ T+++VNAWAIHRD
Sbjct: 351 DESDIAVLPYLQNVVSETFRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRD 402

[Close]

BLAST of Moc11g03330 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 260.0 bits (663), Expect = 2.7e-69
Identity = 137/345 (39.71%), Postives = 203/345 (58.84%), Query Frame = 0


Query: 12  IVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTA----------- 71
           I SLS G R V VVSS + A+ECFTKND++ A+RP  + GK + YN T            
Sbjct: 76  IFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGDSWR 135

Query: 72  ------------------------EEVKLWLKNLYRTTGQINVRVEIRSRLTELSFNIAL 131
                                   +E++  +  L + +    V+VE++     L+ N  +
Sbjct: 136 NLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLTINNII 195

Query: 132 RMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPTDFLSVLKLIDYQGFRKRLWKV 191
           RM+ GKR++G   E+  E + +++++ +V +  GA    D+  +L+ +    + K + K+
Sbjct: 196 RMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TNYEKHVKKL 255

Query: 192 WAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKDHIIKGQILTL 251
               D F QSL+N +R  KV        +   MID +LSLQ++ P YY D IIKG IL +
Sbjct: 256 AGRVDEFLQSLVNEKRVEKV--------KGNTMIDHLLSLQETQPDYYTDVIIKGIILVM 315

Query: 252 LAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQQLVEEADLANLPYLQAIINE 311
           + AGTDT+AGT+EW MS LLNHP  ++KA +EI+D +G  +LVEE D+  LPYLQ I++E
Sbjct: 316 ILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSE 375

Query: 312 TYRMFPALPILVPRESSEDCTIGRYDVPKGTMLVVNAWAIHRDPK 322
           T R++P  P+L+P  +SEDC +  YDVP+GT+++VNAWAIHRDPK
Sbjct: 376 TLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPK 410

The following BLAST results are available for this feature:

BLAST of Moc11g03330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138249.1	9.0e-160	80.90	isoflavone 2'-hydroxylase-like [Momordica charantia]	[more]
XP_022136931.1	7.1e-157	80.34	isoflavone 2'-hydroxylase-like [Momordica charantia]	[more]
XP_022982228.1	1.4e-133	70.31	isoflavone 3'-hydroxylase-like [Cucurbita maxima]	[more]
KAG7037779.1	1.4e-133	70.31	Cytochrome P450 81E8 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022940149.1	1.4e-133	70.31	cytochrome P450 81E8-like [Cucurbita moschata]	[more]

BLAST of Moc11g03330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	2.0e-85	47.89	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q6WNQ9	1.7e-76	44.13	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
P93147	1.3e-73	41.46	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q6WNQ8	3.9e-73	42.78	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
Q0WTF4	3.1e-70	39.50	Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1	[more]

BLAST of Moc11g03330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C9L7	4.3e-160	80.90	isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111009467 PE...	[more]
A0A6J1C4W6	3.4e-157	80.34	isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111008503 PE...	[more]
A0A6J1J4B3	7.0e-134	70.31	isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111481120 PE=3 ...	[more]
A0A6J1FJ78	7.0e-134	70.31	cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111445861 PE=3 SV=...	[more]
A0A0A0L2I1	3.3e-123	61.66	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G641760 PE=3 SV=1	[more]

BLAST of Moc11g03330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37400.1	2.2e-71	39.50	cytochrome P450, family 81, subfamily F, polypeptide 3	[more]
AT4G37330.1	8.3e-71	40.85	cytochrome P450, family 81, subfamily D, polypeptide 4	[more]
AT4G37370.1	1.9e-70	40.17	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37430.1	9.2e-70	38.40	cytochrome P450, family 91, subfamily A, polypeptide 2	[more]
AT3G28740.1	2.7e-69	39.71	Cytochrome P450 superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 6..25 score: 41.8 coord: 228..254 score: 31.25 coord: 30..51 score: 24.21 coord: 271..289 score: 36.95 coord: 208..225 score: 33.26
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 219..236 score: 40.36 coord: 272..283 score: 38.26
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 53..321 e-value: 1.2E-46 score: 159.4 coord: 2..48 e-value: 5.7E-6 score: 25.3
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..323 e-value: 6.6E-73 score: 247.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 2..321
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 61..322
None	No IPR available	PANTHER	PTHR47947:SF27	SUBFAMILY NOT NAMED	coord: 2..60
None	No IPR available	PANTHER	PTHR47947:SF27	SUBFAMILY NOT NAMED	coord: 61..322
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 2..60

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc11g03330.1	Moc11g03330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen