Moc11g02870 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc11g02870
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	protein DCL homolog, chloroplastic
Location	chr11: 1841411 .. 1843195 (+)
RNA-Seq Expression	Moc11g02870
Synteny	Moc11g02870

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATGGCGTCTGTTTCGAAGCCACCGCCATTTCCGCCCTTTCACTCCCTAAATCCAAGCTTTCTCAACTCTTCACCAGTGATCTTATGTTTTCCTATGCACCCAATGACGTCGCTTCACCCCTCCATACGTGCCCTAAAGACTGGGCCGGAAGGCAGTAGAATTCGAAGCCACGAAGAATATAGCTCGGATTTGCTGCGGAAGCCGGTTGTTCCACCGGCGAAGGACTTCGCCAGAATATCGGAGGATGACGAGGGCAGCGAGGAGAGTGGTACTGACGGCGAAGAGGAAGAGGATTGGGTTGATTGGGAGGACAAGATTTTGGAGGATACCGTTCCTCTAGTTGGCTTTGTTAGGATGGTTCTTCACTCTGGAAAGTAAGCATTTATTTGTGTTTCGGCTTTGTAAGTGAATTTGAGCGTGTTCCAGGTCAAATTGAACTTATGCGACTTTGTTATCATGTGATTTTTAAGCTTGTTTTAGCTCAAATAGAAATCGTGTTACTCGAACATGAACGCATTTGAGTTCTATGAGATTGAAATGTGGAATTTTAAAGGAACTTTCTTCGATTTCCATGACAACCAAGAACAATAATTAATGGTCTTATCAATTAATCTCTTTATAAGATGTGTAATTTTAGGATAATGAAGAAAGAGTCCTTGATTTGAAAATCAAATCAATTTTTTCTAATTTGACTATAACGTCGATGCAATGGAAGATTTTAAATCTTTAATAATTGAAGGAAAATGGGGTTTTGTTTGCGATATGATCTGAATGAAAGGGGGTTTTGTTTGATGAGTTCCAGTTGTAATCATCTCAGCATATGCCCTTAAAGACAATTTTAAACATGATTTGCGAGGTATTTTGACTGTATGCTTTGATCATGTAAATGTATTACCCAAATGCTCATTGTTTATAATTCATATAATCAAAGACAGTCAGACTTTTATCTTATCAATGAATAAGATTTCAAGTCCAACTATATGGAACACCTATTATCCGAGAGAAGATGTGGTCACTATCTTGAATTTGTTAGCATGATTAGATATAGTTCAATCTCCATGTGCTCATCATCTCTGATCAAACACGTGCAGATATGGAAGTGGAGATAGATTAAGCCTGGAGCACGAGAAAACGATTCTTGACAGGTTGCTTCCATACCATCCTGAATGTGAGAAGAAGATTGGATGTGGAATTGATTATATCACGGTGTTTTTCTCTCCCCATTTGTTTGCACACTACATACCATGTGTTTTTAGCTTATTCGAAGAATTTAAGTTTACATTAAATTCAAGGTTAAATCATGAACTTTTAATTTTGTATTTAGAATTGAAAGTTTAGGAACCAAGCTTGTAATGTAACCTAAATTAAATAAGTATTTAGATACTCTAGCCTCTTTTCTAATTCCTCAGATGAAAAATGATTCTACCTCTCTTATCCTGAGAAATTAAGGGAAAAGAAGACCTGCTTATGGACAGTGTCATTTTCTTTGTTCAACCATTTTCTTTGTAATTATTTTGAGTCATTTGATGCATACCCATCTCTTTGTCAACTGAAATTATCATAACATTTATGCATTGGTGTCATTGTGGTCGGCAGATTGGATATCATCCTGATTTTGAAAGCTCAAGATGTTTATTCATAGTCAGAAAAGATGGAGAGTTAGTCGATTTCTCATATTGGAAGTGCATTAAGGGTCTGATCAGAAAGAACTATCCCCTCCATGCTGAAAGCTTCATTCTTAGGCATTTTCGGCGACGTAGACGCAATTACCGTGGGTAG

mRNA sequence

ATGGCCATGGCGTCTGTTTCGAAGCCACCGCCATTTCCGCCCTTTCACTCCCTAAATCCAAGCTTTCTCAACTCTTCACCAGTGATCTTATGTTTTCCTATGCACCCAATGACGTCGCTTCACCCCTCCATACGTGCCCTAAAGACTGGGCCGGAAGGCAGTAGAATTCGAAGCCACGAAGAATATAGCTCGGATTTGCTGCGGAAGCCGGTTGTTCCACCGGCGAAGGACTTCGCCAGAATATCGGAGGATGACGAGGGCAGCGAGGAGAGTGGTACTGACGGCGAAGAGGAAGAGGATTGGGTTGATTGGGAGGACAAGATTTTGGAGGATACCGTTCCTCTAGTTGGCTTTGTTAGGATGGTTCTTCACTCTGGAAAATATGGAAGTGGAGATAGATTAAGCCTGGAGCACGAGAAAACGATTCTTGACAGGTTGCTTCCATACCATCCTGAATGTGAGAAGAAGATTGGATGTGGAATTGATTATATCACGATTGGATATCATCCTGATTTTGAAAGCTCAAGATGTTTATTCATAGTCAGAAAAGATGGAGAGTTAGTCGATTTCTCATATTGGAAGTGCATTAAGGGTCTGATCAGAAAGAACTATCCCCTCCATGCTGAAAGCTTCATTCTTAGGCATTTTCGGCGACGTAGACGCAATTACCGTGGGTAG

Coding sequence (CDS)

ATGGCCATGGCGTCTGTTTCGAAGCCACCGCCATTTCCGCCCTTTCACTCCCTAAATCCAAGCTTTCTCAACTCTTCACCAGTGATCTTATGTTTTCCTATGCACCCAATGACGTCGCTTCACCCCTCCATACGTGCCCTAAAGACTGGGCCGGAAGGCAGTAGAATTCGAAGCCACGAAGAATATAGCTCGGATTTGCTGCGGAAGCCGGTTGTTCCACCGGCGAAGGACTTCGCCAGAATATCGGAGGATGACGAGGGCAGCGAGGAGAGTGGTACTGACGGCGAAGAGGAAGAGGATTGGGTTGATTGGGAGGACAAGATTTTGGAGGATACCGTTCCTCTAGTTGGCTTTGTTAGGATGGTTCTTCACTCTGGAAAATATGGAAGTGGAGATAGATTAAGCCTGGAGCACGAGAAAACGATTCTTGACAGGTTGCTTCCATACCATCCTGAATGTGAGAAGAAGATTGGATGTGGAATTGATTATATCACGATTGGATATCATCCTGATTTTGAAAGCTCAAGATGTTTATTCATAGTCAGAAAAGATGGAGAGTTAGTCGATTTCTCATATTGGAAGTGCATTAAGGGTCTGATCAGAAAGAACTATCCCCTCCATGCTGAAAGCTTCATTCTTAGGCATTTTCGGCGACGTAGACGCAATTACCGTGGGTAG

Protein sequence

MAMASVSKPPPFPPFHSLNPSFLNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIRSHEEYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVGFVRMVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFIVRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNYRG

Homology

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BLAST of Moc11g02870 vs. NCBI nr
Match: XP_022133006.1 (protein DCL homolog, chloroplastic [Momordica charantia])

HSP 1 Score: 468.8 bits (1205), Expect = 2.7e-128
Identity = 225/225 (100.00%), Postives = 225/225 (100.00%), Query Frame = 0


Query: 1   MAMASVSKPPPFPPFHSLNPSFLNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIRSHE 60
           MAMASVSKPPPFPPFHSLNPSFLNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIRSHE
Sbjct: 1   MAMASVSKPPPFPPFHSLNPSFLNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIRSHE 60

Query: 61  EYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVGFVR 120
           EYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVGFVR 120

Query: 121 MVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFI 180
           MVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFI
Sbjct: 121 MVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFI 180

Query: 181 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNYRG 226
           VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNYRG
Sbjct: 181 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNYRG 225

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BLAST of Moc11g02870 vs. NCBI nr
Match: XP_038897137.1 (protein DCL homolog, chloroplastic [Benincasa hispida])

HSP 1 Score: 417.5 bits (1072), Expect = 7.1e-113
Identity = 201/224 (89.73%), Postives = 208/224 (92.86%), Query Frame = 0


Query: 1   MAMASVSKPPPFPPFHSLNPSFLNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIRSHE 60
           MAMAS+ KPPPF P HSLNP+F NSSPVILCFP  PMTS H S RALKTGPEGSRIRSHE
Sbjct: 3   MAMASILKPPPFVPSHSLNPNFFNSSPVILCFPTQPMTSFHLSTRALKTGPEGSRIRSHE 62

Query: 61  EYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVGFVR 120
           EYSSDLLRKPVVPPA+D AR+SEDD+ SEESG D EEEEDWVDWEDKILEDTVPLVGFVR
Sbjct: 63  EYSSDLLRKPVVPPARDLARLSEDDDSSEESGNDDEEEEDWVDWEDKILEDTVPLVGFVR 122

Query: 121 MVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFI 180
           MVLHSGKYGSGDRL+ EHEKTILDRLLPYHPE EKKIGCGIDYITIGYHPDFESSRCLFI
Sbjct: 123 MVLHSGKYGSGDRLNPEHEKTILDRLLPYHPESEKKIGCGIDYITIGYHPDFESSRCLFI 182

Query: 181 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNYR 225
           VRKDGELVDFSYWKCIKGLIRK YPLHAESFILRHFRRRRRN+R
Sbjct: 183 VRKDGELVDFSYWKCIKGLIRKKYPLHAESFILRHFRRRRRNFR 226

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BLAST of Moc11g02870 vs. NCBI nr
Match: XP_008452556.1 (PREDICTED: protein DCL, chloroplastic [Cucumis melo])

HSP 1 Score: 399.8 bits (1026), Expect = 1.5e-107
Identity = 192/224 (85.71%), Postives = 203/224 (90.62%), Query Frame = 0


Query: 1   MAMASVSKPPPFPPFHSLNPSFLNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIRSHE 60
           MAMAS+    PFPPFHSLNP+F NSSP+ILCFP HP+ S HPS RALKTGPEGSRIRSH+
Sbjct: 1   MAMASILTLLPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGSRIRSHQ 60

Query: 61  EYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVGFVR 120
           EYSSDLLRKPVVPPAKD A  SEDD+ SEESG D EEE DWVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVVPPAKDLAGPSEDDDSSEESGNDEEEEVDWVDWEDKILEDTVPLVGFVR 120

Query: 121 MVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFI 180
           MVLH+GKY SGDRL  EHEKTIL+RLLPYHPE EKKIGCG+DYIT+GYHPDFESSRCLFI
Sbjct: 121 MVLHTGKYESGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 181 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNYR 225
           VRKDGELVDFSYWKCIKGLIRKNYPL+AESFILRHFRRRRRN R
Sbjct: 181 VRKDGELVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRNLR 224

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BLAST of Moc11g02870 vs. NCBI nr
Match: KAA0064386.1 (protein DCL [Cucumis melo var. makuwa] >TYK20201.1 protein DCL [Cucumis melo var. makuwa])

HSP 1 Score: 399.8 bits (1026), Expect = 1.5e-107
Identity = 192/224 (85.71%), Postives = 203/224 (90.62%), Query Frame = 0


Query: 1   MAMASVSKPPPFPPFHSLNPSFLNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIRSHE 60
           MAMAS+    PFPPFHSLNP+F NSSP+ILCFP HP+ S HPS RALKTGPEGSRIRSH+
Sbjct: 1   MAMASILTLLPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGSRIRSHQ 60

Query: 61  EYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVGFVR 120
           EYSSDLLRKPVVPPAKD A  SEDD+ SEESG D EEE DWVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVVPPAKDLAGPSEDDDSSEESGNDEEEEVDWVDWEDKILEDTVPLVGFVR 120

Query: 121 MVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFI 180
           MVLH+GKY SGDRL  EHEKTIL+RLLPYHPE EKKIGCG+DYIT+GYHPDFESSRCLFI
Sbjct: 121 MVLHTGKYESGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 181 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNYR 225
           VRKDGELVDFSYWKCIKGLIRKNYPL+AESFILRHFRRRRRN R
Sbjct: 181 VRKDGELVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRNLR 224

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BLAST of Moc11g02870 vs. NCBI nr
Match: XP_004141259.1 (protein DCL homolog, chloroplastic [Cucumis sativus] >KGN55194.1 hypothetical protein Csa_011993 [Cucumis sativus])

HSP 1 Score: 396.0 bits (1016), Expect = 2.2e-106
Identity = 187/224 (83.48%), Postives = 203/224 (90.62%), Query Frame = 0


Query: 1   MAMASVSKPPPFPPFHSLNPSFLNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIRSHE 60
           MAMAS+ KPPPFPPFHSLNP+F NSSP+ILCFP HP+ S HPS RALKTGPEG R+RSH+
Sbjct: 1   MAMASILKPPPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIRLRSHQ 60

Query: 61  EYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVGFVR 120
           EYSSDLLRKPV P AKD A  SEDD+ SEESG + EEE +WVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVR 120

Query: 121 MVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFI 180
           MVLH+GKY +GDRL  EHEKTIL+RLLPYHPE EKKIGCG+DYIT+GYHPDFESSRCLFI
Sbjct: 121 MVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 181 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNYR 225
           VRKDGE+VDFSYWKCIKGLIRKNYPL+AESFILRHFRRRRRN R
Sbjct: 181 VRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRNSR 224

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BLAST of Moc11g02870 vs. ExPASy Swiss-Prot
Match: Q9C642 (Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 2.8e-72
Identity = 138/225 (61.33%), Postives = 173/225 (76.89%), Query Frame = 0


Query: 1   MAMASV--SKPPPFPPFHSLNPSF-LNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIR 60
           M++AS+  S P   P F      F  +SSP+ L FP    TSL P +RAL+T  +G++I 
Sbjct: 1   MSLASIPSSSPVASPYFRCRTYIFSFSSSPLCLYFPRGDSTSLRPRVRALRTESDGAKIG 60

Query: 61  SHEEYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVG 120
           + E Y S+LLR+P +   +     SE++E  EE  ++G+E   +VDWEDKILE TVPLVG
Sbjct: 61  NSESYGSELLRRPRIASEES----SEEEEEEEEENSEGDE---FVDWEDKILEVTVPLVG 120

Query: 121 FVRMVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRC 180
           FVRM+LHSGKY + DRLS EHE+TI++ LLPYHPECEKKIGCGIDYI +G+HPDFESSRC
Sbjct: 121 FVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKIGCGIDYIMVGHHPDFESSRC 180

Query: 181 LFIVRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRN 223
           +FIVRKDGE+VDFSYWKCIKGLI+K YPL+A+SFILRHFR+RR+N
Sbjct: 181 MFIVRKDGEVVDFSYWKCIKGLIKKKYPLYADSFILRHFRKRRQN 218

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BLAST of Moc11g02870 vs. ExPASy Swiss-Prot
Match: Q42463 (Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 4.2e-68
Identity = 125/180 (69.44%), Postives = 147/180 (81.67%), Query Frame = 0


Query: 46  ALKTGPEGSRIRSHEEYSSDLLRKPVVPPAKDFARISED---DEGSEESGTDGEEEEDWV 105
           A+KTG EG  IRS    +++LLRKPV+    +    SE+   +E  +E G    + E WV
Sbjct: 47  AVKTGSEGGGIRSD---NAELLRKPVISTELETTSESEELVKEESDDEVGKKSGDGEGWV 106

Query: 106 DWEDKILEDTVPLVGFVRMVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGID 165
           DWED+ILEDTVPLVGFVRM+LHSGKY  GDRLS +H++TIL RLLPYHPEC+KKIG G+D
Sbjct: 107 DWEDQILEDTVPLVGFVRMILHSGKYAIGDRLSPDHQRTILQRLLPYHPECDKKIGPGVD 166

Query: 166 YITIGYHPDFESSRCLFIVRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRN 223
           YIT+GYHPDFE+SRCLFIVRKDGE VDFSYWKCIKGLIRKNYPL+A+SFILRHFR+RRRN
Sbjct: 167 YITVGYHPDFENSRCLFIVRKDGETVDFSYWKCIKGLIRKNYPLYADSFILRHFRKRRRN 223

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BLAST of Moc11g02870 vs. ExPASy Swiss-Prot
Match: Q5D869 (DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPE1 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 6.6e-21
Identity = 44/116 (37.93%), Postives = 70/116 (60.34%), Query Frame = 0


Query: 105  EDKILEDTVPLVGFVRMVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYI 164
            E ++L D  P++  +R ++H   Y  GD +S + +  +L+++L +HP+ E K+G G+D+I
Sbjct: 1736 EQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVDFI 1795

Query: 165  TIGYHPDFESSRCLFIVRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRR 221
            T+  H  F  SRC F+V  DG   DFSY K +   + K YP  AE FI ++F + R
Sbjct: 1796 TVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKYFTKPR 1851

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BLAST of Moc11g02870 vs. ExPASy Swiss-Prot
Match: Q8L557 (Protein EMBRYO DEFECTIVE 514 OS=Arabidopsis thaliana OX=3702 GN=EMB514 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 6.7e-05
Identity = 39/119 (32.77%), Postives = 57/119 (47.90%), Query Frame = 0


Query: 83  EDDEGSEESGTDGEEEEDW-VDWEDKILEDTVPLVGFVRMVLHSGKYGSGDRLSLEHEKT 142
           E++ G  +    GEEE+   V    K    +V +  +    LH       D    EH   
Sbjct: 47  EENGGESKKQKVGEEEKSGPVKLGPKEFVTSVAMFDYFVKFLHFWPTDL-DVNKYEH-MV 106

Query: 143 ILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFIVRKDGELVDFSYWKCIKGLI 201
           +LD +   H E EKKIG GI    +  HP ++ SRC F+VR+D    DFS+ KC+  ++
Sbjct: 107 LLDLIKKGHSEPEKKIGGGIKTFQVRTHPMWK-SRCFFLVREDDTADDFSFRKCVDQIL 162

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BLAST of Moc11g02870 vs. ExPASy Swiss-Prot
Match: P85936 (Protein DCL, chloroplastic (Fragment) OS=Pseudotsuga menziesii OX=3357 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 7.4e-04
Identity = 18/22 (81.82%), Postives = 21/22 (95.45%), Query Frame = 0


Query: 101 WVDWEDKILEDTVPLVGFVRMV 123
           WVDWED+IL+DTVPLV FVRM+
Sbjct: 2   WVDWEDQILQDTVPLVNFVRMI 23

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BLAST of Moc11g02870 vs. ExPASy TrEMBL
Match: A0A6J1BTU2 (protein DCL homolog, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111005680 PE=4 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 1.3e-128
Identity = 225/225 (100.00%), Postives = 225/225 (100.00%), Query Frame = 0


Query: 1   MAMASVSKPPPFPPFHSLNPSFLNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIRSHE 60
           MAMASVSKPPPFPPFHSLNPSFLNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIRSHE
Sbjct: 1   MAMASVSKPPPFPPFHSLNPSFLNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIRSHE 60

Query: 61  EYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVGFVR 120
           EYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVGFVR 120

Query: 121 MVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFI 180
           MVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFI
Sbjct: 121 MVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFI 180

Query: 181 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNYRG 226
           VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNYRG
Sbjct: 181 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNYRG 225

[Close]

BLAST of Moc11g02870 vs. ExPASy TrEMBL
Match: A0A5A7VDC9 (Protein DCL OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003370 PE=4 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 7.4e-108
Identity = 192/224 (85.71%), Postives = 203/224 (90.62%), Query Frame = 0


Query: 1   MAMASVSKPPPFPPFHSLNPSFLNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIRSHE 60
           MAMAS+    PFPPFHSLNP+F NSSP+ILCFP HP+ S HPS RALKTGPEGSRIRSH+
Sbjct: 1   MAMASILTLLPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGSRIRSHQ 60

Query: 61  EYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVGFVR 120
           EYSSDLLRKPVVPPAKD A  SEDD+ SEESG D EEE DWVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVVPPAKDLAGPSEDDDSSEESGNDEEEEVDWVDWEDKILEDTVPLVGFVR 120

Query: 121 MVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFI 180
           MVLH+GKY SGDRL  EHEKTIL+RLLPYHPE EKKIGCG+DYIT+GYHPDFESSRCLFI
Sbjct: 121 MVLHTGKYESGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 181 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNYR 225
           VRKDGELVDFSYWKCIKGLIRKNYPL+AESFILRHFRRRRRN R
Sbjct: 181 VRKDGELVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRNLR 224

[Close]

BLAST of Moc11g02870 vs. ExPASy TrEMBL
Match: A0A1S3BVA4 (protein DCL, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493543 PE=4 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 7.4e-108
Identity = 192/224 (85.71%), Postives = 203/224 (90.62%), Query Frame = 0


Query: 1   MAMASVSKPPPFPPFHSLNPSFLNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIRSHE 60
           MAMAS+    PFPPFHSLNP+F NSSP+ILCFP HP+ S HPS RALKTGPEGSRIRSH+
Sbjct: 1   MAMASILTLLPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGSRIRSHQ 60

Query: 61  EYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVGFVR 120
           EYSSDLLRKPVVPPAKD A  SEDD+ SEESG D EEE DWVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVVPPAKDLAGPSEDDDSSEESGNDEEEEVDWVDWEDKILEDTVPLVGFVR 120

Query: 121 MVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFI 180
           MVLH+GKY SGDRL  EHEKTIL+RLLPYHPE EKKIGCG+DYIT+GYHPDFESSRCLFI
Sbjct: 121 MVLHTGKYESGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 181 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNYR 225
           VRKDGELVDFSYWKCIKGLIRKNYPL+AESFILRHFRRRRRN R
Sbjct: 181 VRKDGELVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRNLR 224

[Close]

BLAST of Moc11g02870 vs. ExPASy TrEMBL
Match: A0A0A0L2D0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639820 PE=4 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 1.1e-106
Identity = 187/224 (83.48%), Postives = 203/224 (90.62%), Query Frame = 0


Query: 1   MAMASVSKPPPFPPFHSLNPSFLNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIRSHE 60
           MAMAS+ KPPPFPPFHSLNP+F NSSP+ILCFP HP+ S HPS RALKTGPEG R+RSH+
Sbjct: 1   MAMASILKPPPFPPFHSLNPNFFNSSPLILCFPTHPINSFHPSTRALKTGPEGIRLRSHQ 60

Query: 61  EYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVGFVR 120
           EYSSDLLRKPV P AKD A  SEDD+ SEESG + EEE +WVDWEDKILEDTVPLVGFVR
Sbjct: 61  EYSSDLLRKPVGPSAKDLAGPSEDDDSSEESGNEDEEEVEWVDWEDKILEDTVPLVGFVR 120

Query: 121 MVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFI 180
           MVLH+GKY +GDRL  EHEKTIL+RLLPYHPE EKKIGCG+DYIT+GYHPDFESSRCLFI
Sbjct: 121 MVLHTGKYENGDRLRPEHEKTILERLLPYHPESEKKIGCGVDYITVGYHPDFESSRCLFI 180

Query: 181 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNYR 225
           VRKDGE+VDFSYWKCIKGLIRKNYPL+AESFILRHFRRRRRN R
Sbjct: 181 VRKDGEMVDFSYWKCIKGLIRKNYPLYAESFILRHFRRRRRNSR 224

[Close]

BLAST of Moc11g02870 vs. ExPASy TrEMBL
Match: A0A6J1IM57 (protein DCL homolog, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476513 PE=4 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 9.1e-106
Identity = 188/223 (84.30%), Postives = 202/223 (90.58%), Query Frame = 0


Query: 1   MAMASVSKPPPFPPFHSLNPSFLNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIRSHE 60
           +AMAS+ KPPP  P HSLNP+ LN+SPVI+CF  HPMTS HPSIRALKTGPEGSRIRSHE
Sbjct: 3   LAMASILKPPPLVPLHSLNPNLLNASPVIVCFRTHPMTSFHPSIRALKTGPEGSRIRSHE 62

Query: 61  EYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVGFVR 120
           EYSSDLLRKPVVPPA D A  SED   SEESG   EEEE+WVDWED+ILE+TVPLVGFVR
Sbjct: 63  EYSSDLLRKPVVPPAMDLATPSEDYNSSEESGNGDEEEEEWVDWEDRILEETVPLVGFVR 122

Query: 121 MVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFI 180
           MVLHSGKYGSGDRLS EHEKTILDRLL YHPECEKKIGCGIDYITIGYHP+FE SRCLFI
Sbjct: 123 MVLHSGKYGSGDRLSPEHEKTILDRLLLYHPECEKKIGCGIDYITIGYHPNFERSRCLFI 182

Query: 181 VRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRNY 224
           VRKDGELVDFSYWKC++GLIRKNYPLHAESFILRHF+RRRR++
Sbjct: 183 VRKDGELVDFSYWKCMEGLIRKNYPLHAESFILRHFQRRRRHF 225

[Close]

BLAST of Moc11g02870 vs. TAIR 10
Match: AT1G45230.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 273.1 bits (697), Expect = 2.0e-73
Identity = 138/225 (61.33%), Postives = 173/225 (76.89%), Query Frame = 0


Query: 1   MAMASV--SKPPPFPPFHSLNPSF-LNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIR 60
           M++AS+  S P   P F      F  +SSP+ L FP    TSL P +RAL+T  +G++I 
Sbjct: 1   MSLASIPSSSPVASPYFRCRTYIFSFSSSPLCLYFPRGDSTSLRPRVRALRTESDGAKIG 60

Query: 61  SHEEYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVG 120
           + E Y S+LLR+P +   +     SE++E  EE  ++G+E   +VDWEDKILE TVPLVG
Sbjct: 61  NSESYGSELLRRPRIASEES----SEEEEEEEEENSEGDE---FVDWEDKILEVTVPLVG 120

Query: 121 FVRMVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRC 180
           FVRM+LHSGKY + DRLS EHE+TI++ LLPYHPECEKKIGCGIDYI +G+HPDFESSRC
Sbjct: 121 FVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKIGCGIDYIMVGHHPDFESSRC 180

Query: 181 LFIVRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRN 223
           +FIVRKDGE+VDFSYWKCIKGLI+K YPL+A+SFILRHFR+RR+N
Sbjct: 181 MFIVRKDGEVVDFSYWKCIKGLIKKKYPLYADSFILRHFRKRRQN 218

[Close]

BLAST of Moc11g02870 vs. TAIR 10
Match: AT1G45230.2 (Protein of unknown function (DUF3223) )

HSP 1 Score: 270.0 bits (689), Expect = 1.7e-72
Identity = 137/225 (60.89%), Postives = 172/225 (76.44%), Query Frame = 0


Query: 1   MAMASV--SKPPPFPPFHSLNPSF-LNSSPVILCFPMHPMTSLHPSIRALKTGPEGSRIR 60
           M++AS+  S P   P F      F  +SSP+ L FP    TSL P +RAL+T  +G++I 
Sbjct: 1   MSLASIPSSSPVASPYFRCRTYIFSFSSSPLCLYFPRGDSTSLRPRVRALRTESDGAKIG 60

Query: 61  SHEEYSSDLLRKPVVPPAKDFARISEDDEGSEESGTDGEEEEDWVDWEDKILEDTVPLVG 120
           + E Y S+LLR+P +   +     SE++E  EE  ++G+E   +VDWEDKILE TVPLVG
Sbjct: 61  NSESYGSELLRRPRIASEES----SEEEEEEEEENSEGDE---FVDWEDKILEVTVPLVG 120

Query: 121 FVRMVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRC 180
           FVRM+LHSGKY + DRLS EHE+TI++ LLPYHPECEKKIGCGIDYI + +HPDFESSRC
Sbjct: 121 FVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKIGCGIDYIMVWHHPDFESSRC 180

Query: 181 LFIVRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRRRN 223
           +FIVRKDGE+VDFSYWKCIKGLI+K YPL+A+SFILRHFR+RR+N
Sbjct: 181 MFIVRKDGEVVDFSYWKCIKGLIKKKYPLYADSFILRHFRKRRQN 218

[Close]

BLAST of Moc11g02870 vs. TAIR 10
Match: AT3G46630.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 139.4 bits (350), Expect = 3.5e-33
Identity = 58/118 (49.15%), Postives = 79/118 (66.95%), Query Frame = 0


Query: 101 WVDWEDKILEDTVPLVGFVRMVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCG 160
           W + E +IL D  P+    + +LHS +Y  G+RL  E EK ++++LLPYHP  + KIGCG
Sbjct: 87  WKNLEAEILRDIEPISLLAKEILHSDRYLDGERLDFEDEKIVMEKLLPYHPYSKDKIGCG 146

Query: 161 IDYITIGYHPDFESSRCLFIVRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRR 219
           +D+I +  HP F  SRCLF+VR DG  +DFSY KC++  +R  YP HAE FI  HF+R
Sbjct: 147 LDFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYVRDKYPSHAERFIREHFKR 204

[Close]

BLAST of Moc11g02870 vs. TAIR 10
Match: AT2G40030.1 (nuclear RNA polymerase D1B )

HSP 1 Score: 102.4 bits (254), Expect = 4.7e-22
Identity = 44/116 (37.93%), Postives = 70/116 (60.34%), Query Frame = 0


Query: 105  EDKILEDTVPLVGFVRMVLHSGKYGSGDRLSLEHEKTILDRLLPYHPECEKKIGCGIDYI 164
            E ++L D  P++  +R ++H   Y  GD +S + +  +L+++L +HP+ E K+G G+D+I
Sbjct: 1736 EQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVDFI 1795

Query: 165  TIGYHPDFESSRCLFIVRKDGELVDFSYWKCIKGLIRKNYPLHAESFILRHFRRRR 221
            T+  H  F  SRC F+V  DG   DFSY K +   + K YP  AE FI ++F + R
Sbjct: 1796 TVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKYFTKPR 1851

[Close]

BLAST of Moc11g02870 vs. TAIR 10
Match: AT5G62440.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 49.3 bits (116), Expect = 4.7e-06
Identity = 39/119 (32.77%), Postives = 57/119 (47.90%), Query Frame = 0


Query: 83  EDDEGSEESGTDGEEEEDW-VDWEDKILEDTVPLVGFVRMVLHSGKYGSGDRLSLEHEKT 142
           E++ G  +    GEEE+   V    K    +V +  +    LH       D    EH   
Sbjct: 47  EENGGESKKQKVGEEEKSGPVKLGPKEFVTSVAMFDYFVKFLHFWPTDL-DVNKYEH-MV 106

Query: 143 ILDRLLPYHPECEKKIGCGIDYITIGYHPDFESSRCLFIVRKDGELVDFSYWKCIKGLI 201
           +LD +   H E EKKIG GI    +  HP ++ SRC F+VR+D    DFS+ KC+  ++
Sbjct: 107 LLDLIKKGHSEPEKKIGGGIKTFQVRTHPMWK-SRCFFLVREDDTADDFSFRKCVDQIL 162

The following BLAST results are available for this feature:

BLAST of Moc11g02870 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133006.1	2.7e-128	100.00	protein DCL homolog, chloroplastic [Momordica charantia]	[more]
XP_038897137.1	7.1e-113	89.73	protein DCL homolog, chloroplastic [Benincasa hispida]	[more]
XP_008452556.1	1.5e-107	85.71	PREDICTED: protein DCL, chloroplastic [Cucumis melo]	[more]
KAA0064386.1	1.5e-107	85.71	protein DCL [Cucumis melo var. makuwa] >TYK20201.1 protein DCL [Cucumis melo var...	[more]
XP_004141259.1	2.2e-106	83.48	protein DCL homolog, chloroplastic [Cucumis sativus] >KGN55194.1 hypothetical pr...	[more]

BLAST of Moc11g02870 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C642	2.8e-72	61.33	Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 S...	[more]
Q42463	4.2e-68	69.44	Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1	[more]
Q5D869	6.6e-21	37.93	DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPE1...	[more]
Q8L557	6.7e-05	32.77	Protein EMBRYO DEFECTIVE 514 OS=Arabidopsis thaliana OX=3702 GN=EMB514 PE=1 SV=1	[more]
P85936	7.4e-04	81.82	Protein DCL, chloroplastic (Fragment) OS=Pseudotsuga menziesii OX=3357 PE=1 SV=1	[more]

BLAST of Moc11g02870 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BTU2	1.3e-128	100.00	protein DCL homolog, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11100568...	[more]
A0A5A7VDC9	7.4e-108	85.71	Protein DCL OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003370 P...	[more]
A0A1S3BVA4	7.4e-108	85.71	protein DCL, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493543 PE=4 SV=1	[more]
A0A0A0L2D0	1.1e-106	83.48	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639820 PE=4 SV=1	[more]
A0A6J1IM57	9.1e-106	84.30	protein DCL homolog, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476...	[more]

BLAST of Moc11g02870 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G45230.1	2.0e-73	61.33	Protein of unknown function (DUF3223)	[more]
AT1G45230.2	1.7e-72	60.89	Protein of unknown function (DUF3223)	[more]
AT3G46630.1	3.5e-33	49.15	Protein of unknown function (DUF3223)	[more]
AT2G40030.1	4.7e-22	37.93	nuclear RNA polymerase D1B	[more]
AT5G62440.1	4.7e-06	32.77	Protein of unknown function (DUF3223)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF11523	DUF3223	coord: 121..196 e-value: 2.3E-28 score: 98.7
None	No IPR available	GENE3D	3.10.450.40		coord: 110..199 e-value: 8.4E-25 score: 89.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 77..99
None	No IPR available	PANTHER	PTHR33415:SF15	PROTEIN DCL HOMOLOG, CHLOROPLASTIC	coord: 18..222
IPR044673	Protein DCL-like	PANTHER	PTHR33415	FAMILY NOT NAMED	coord: 18..222

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc11g02870.1	Moc11g02870.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009658	chloroplast organization
biological_process	GO:1901259	chloroplast rRNA processing
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0003729	mRNA binding