Moc11g00840 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc11g00840
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	tRNA pseudouridine synthase family protein
Location	chr11: 549524 .. 551082 (+)
RNA-Seq Expression	Moc11g00840
Synteny	Moc11g00840

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGTCCACAACCACCACCTCTGACGACGAAGATGTCAATCCCAATTCCGCGGCACCGAAGAAACAGAGGTACAAGCGCCGCAAGATCGCTATTTTCTTCGCCTACTGTGGCGTCGGTTACCAAGGGATGCAGAAGAATCCGGGCGCCAAAACCATCGAAGCCGACCTCGAAGAAGCCTTATATCTTGCCGGAGCAGTCCCGGAGCACGACCGCGGGCTCTCAAAGCGCTACGACTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTAAGTGCCGTGGGTCAGGTTGTTTCCGGCCGCTTCTACATCGATCCGCCTGGTTTTGTTGATCGTCTCAATTCGAATCTCTCGCCTCAAATACGGATTTTTGGGTACAAGCGCGTCACAGCGTCATTCAATGCAAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATTCCCGTCTTTGCTCTCAACCCTAATTCCCATCGTGATAGAGAAAGTGTGAAGGCTAGTTTAGGGTCTGATAACGAGCTTGTAAAGTGCTTAGAATGCTCTGAACGAGGGCGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTTGAAGCAAAAACTGTGATAACCGAGTCAGAAGTTTCGTCGAACACTGGAATCGCCGTTGAAGTTTCTGAAGGGGCCTTGGGGGACAATACCCCAGCCTCTGATTCTAACAAACAAACTAGCGTTTTAGTTGAAAATGGCGACACTGAACTTAATCTAGGAAGCGAAGGTACTGTAAAATGTGAATGCAATGAAACGTCTGGATTAGGGAATGACTCAAAGACTGTGGTAAGCAAAGAGAGCGTCGATGGGAATGAGAAACCTTCAGGGAGCTTCAAGTATGGCGAGGAGGAGAAGGCTAGGTTCAATAGGATTCTGAAGCATTATGTAGGAACTCATAACTTCCATAACTTCACTACCAGAACGAAAGCAGAGGACCCTGCTGCTCGACGCTTTATTGTTTCATTTGATGCGGCTACCACCGTTGTGGTTGAAGGTATTGAATTTGTAAAATGTGAAGTTGTGGGGCAGAGCTTCATGCTTCATCAGATCCGGAAGATGATGGGTCTTGCTGTGGCAATTATGAGGAACTGTGCTCCAGAGTCATTGATCGAAAAAGCTTTGCAAAAGTAAGTAAATCACCACTAAATTTTGACTTGATTCTGCCTTTGTTAATAGAATTGGATTTAATCAACTCTTAGTAATCAAACTTCAAACACAGGGATGTTAACATAAATGTACCCACTGCACCTGAGGTTGGGCTGTACTTGGAGGAGTGCCTATTCACATCATATAACCAGAAGTGGAAGGAGAGTCATGAAGAGGTATCTATGAAAGCCTATGAAGAGGTAGCAGAAGACTTCAAAATGAAGCAAATATATTCTCATATTGCTTCCACAGAGCACAAAGAGGGAGCTGTTGCTTTATGGCTGCATTCTCTAAACCATCGCAATTATCCAGATTTACGTGTTGCTGATGAGGTTGCAGATAAAGCTAATGACACTTGCGTTGGCATGGAGACACCAGCTGAAATTTAA

mRNA sequence

ATGTCGTCCACAACCACCACCTCTGACGACGAAGATGTCAATCCCAATTCCGCGGCACCGAAGAAACAGAGGTACAAGCGCCGCAAGATCGCTATTTTCTTCGCCTACTGTGGCGTCGGTTACCAAGGGATGCAGAAGAATCCGGGCGCCAAAACCATCGAAGCCGACCTCGAAGAAGCCTTATATCTTGCCGGAGCAGTCCCGGAGCACGACCGCGGGCTCTCAAAGCGCTACGACTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTAAGTGCCGTGGGTCAGGTTGTTTCCGGCCGCTTCTACATCGATCCGCCTGGTTTTGTTGATCGTCTCAATTCGAATCTCTCGCCTCAAATACGGATTTTTGGGTACAAGCGCGTCACAGCGTCATTCAATGCAAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATTCCCGTCTTTGCTCTCAACCCTAATTCCCATCGTGATAGAGAAAGTGTGAAGGCTAGTTTAGGGTCTGATAACGAGCTTGTAAAGTGCTTAGAATGCTCTGAACGAGGGCGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTTGAAGCAAAAACTGTGATAACCGAGTCAGAAGTTTCGTCGAACACTGGAATCGCCGTTGAAGTTTCTGAAGGGGCCTTGGGGGACAATACCCCAGCCTCTGATTCTAACAAACAAACTAGCGTTTTAGTTGAAAATGGCGACACTGAACTTAATCTAGGAAGCGAAGGTACTGTAAAATGTGAATGCAATGAAACGTCTGGATTAGGGAATGACTCAAAGACTGTGGTAAGCAAAGAGAGCGTCGATGGGAATGAGAAACCTTCAGGGAGCTTCAAGTATGGCGAGGAGGAGAAGGCTAGGTTCAATAGGATTCTGAAGCATTATGTAGGAACTCATAACTTCCATAACTTCACTACCAGAACGAAAGCAGAGGACCCTGCTGCTCGACGCTTTATTGTTTCATTTGATGCGGCTACCACCGTTGTGGTTGAAGGTATTGAATTTGTAAAATGTGAAGTTGTGGGGCAGAGCTTCATGCTTCATCAGATCCGGAAGATGATGGGTCTTGCTGTGGCAATTATGAGGAACTGTGCTCCAGAGTCATTGATCGAAAAAGCTTTGCAAAAGGATGTTAACATAAATGTACCCACTGCACCTGAGGTTGGGCTGTACTTGGAGGAGTGCCTATTCACATCATATAACCAGAAGTGGAAGGAGAGTCATGAAGAGGTATCTATGAAAGCCTATGAAGAGGTAGCAGAAGACTTCAAAATGAAGCAAATATATTCTCATATTGCTTCCACAGAGCACAAAGAGGGAGCTGTTGCTTTATGGCTGCATTCTCTAAACCATCGCAATTATCCAGATTTACGTGTTGCTGATGAGGTTGCAGATAAAGCTAATGACACTTGCGTTGGCATGGAGACACCAGCTGAAATTTAA

Coding sequence (CDS)

ATGTCGTCCACAACCACCACCTCTGACGACGAAGATGTCAATCCCAATTCCGCGGCACCGAAGAAACAGAGGTACAAGCGCCGCAAGATCGCTATTTTCTTCGCCTACTGTGGCGTCGGTTACCAAGGGATGCAGAAGAATCCGGGCGCCAAAACCATCGAAGCCGACCTCGAAGAAGCCTTATATCTTGCCGGAGCAGTCCCGGAGCACGACCGCGGGCTCTCAAAGCGCTACGACTGGGCCCGCTCGGCTCGCACTGATAAGGGCGTAAGTGCCGTGGGTCAGGTTGTTTCCGGCCGCTTCTACATCGATCCGCCTGGTTTTGTTGATCGTCTCAATTCGAATCTCTCGCCTCAAATACGGATTTTTGGGTACAAGCGCGTCACAGCGTCATTCAATGCAAAAAAGTTCTGCGATCGGAGAAGGTATGTATACCTAATTCCCGTCTTTGCTCTCAACCCTAATTCCCATCGTGATAGAGAAAGTGTGAAGGCTAGTTTAGGGTCTGATAACGAGCTTGTAAAGTGCTTAGAATGCTCTGAACGAGGGCGCAAGGTCGAAGGCTTGATGGGCAAGCGCAATTTTGAAGCAAAAACTGTGATAACCGAGTCAGAAGTTTCGTCGAACACTGGAATCGCCGTTGAAGTTTCTGAAGGGGCCTTGGGGGACAATACCCCAGCCTCTGATTCTAACAAACAAACTAGCGTTTTAGTTGAAAATGGCGACACTGAACTTAATCTAGGAAGCGAAGGTACTGTAAAATGTGAATGCAATGAAACGTCTGGATTAGGGAATGACTCAAAGACTGTGGTAAGCAAAGAGAGCGTCGATGGGAATGAGAAACCTTCAGGGAGCTTCAAGTATGGCGAGGAGGAGAAGGCTAGGTTCAATAGGATTCTGAAGCATTATGTAGGAACTCATAACTTCCATAACTTCACTACCAGAACGAAAGCAGAGGACCCTGCTGCTCGACGCTTTATTGTTTCATTTGATGCGGCTACCACCGTTGTGGTTGAAGGTATTGAATTTGTAAAATGTGAAGTTGTGGGGCAGAGCTTCATGCTTCATCAGATCCGGAAGATGATGGGTCTTGCTGTGGCAATTATGAGGAACTGTGCTCCAGAGTCATTGATCGAAAAAGCTTTGCAAAAGGATGTTAACATAAATGTACCCACTGCACCTGAGGTTGGGCTGTACTTGGAGGAGTGCCTATTCACATCATATAACCAGAAGTGGAAGGAGAGTCATGAAGAGGTATCTATGAAAGCCTATGAAGAGGTAGCAGAAGACTTCAAAATGAAGCAAATATATTCTCATATTGCTTCCACAGAGCACAAAGAGGGAGCTGTTGCTTTATGGCTGCATTCTCTAAACCATCGCAATTATCCAGATTTACGTGTTGCTGATGAGGTTGCAGATAAAGCTAATGACACTTGCGTTGGCATGGAGACACCAGCTGAAATTTAA

Protein sequence

MSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTVITESEVSSNTGIAVEVSEGALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLRVADEVADKANDTCVGMETPAEI

Homology

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BLAST of Moc11g00840 vs. NCBI nr
Match: XP_022132914.1 (tRNA pseudouridine synthase A, mitochondrial-like [Momordica charantia])

HSP 1 Score: 969.5 bits (2505), Expect = 1.1e-278
Identity = 488/488 (100.00%), Postives = 488/488 (100.00%), Query Frame = 0


Query: 1   MSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 60
           MSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEA
Sbjct: 29  MSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 88

Query: 61  LYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 120
           LYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI
Sbjct: 89  LYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 148

Query: 121 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECS 180
           RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECS
Sbjct: 149 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECS 208

Query: 181 ERGRKVEGLMGKRNFEAKTVITESEVSSNTGIAVEVSEGALGDNTPASDSNKQTSVLVEN 240
           ERGRKVEGLMGKRNFEAKTVITESEVSSNTGIAVEVSEGALGDNTPASDSNKQTSVLVEN
Sbjct: 209 ERGRKVEGLMGKRNFEAKTVITESEVSSNTGIAVEVSEGALGDNTPASDSNKQTSVLVEN 268

Query: 241 GDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARFNRIL 300
           GDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARFNRIL
Sbjct: 269 GDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARFNRIL 328

Query: 301 KHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRK 360
           KHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRK
Sbjct: 329 KHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRK 388

Query: 361 MMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESHEEVS 420
           MMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESHEEVS
Sbjct: 389 MMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESHEEVS 448

Query: 421 MKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLRVADEVADKANDTCV 480
           MKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLRVADEVADKANDTCV
Sbjct: 449 MKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLRVADEVADKANDTCV 508

Query: 481 GMETPAEI 489
           GMETPAEI
Sbjct: 509 GMETPAEI 516

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BLAST of Moc11g00840 vs. NCBI nr
Match: XP_008452911.1 (PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >XP_008452912.1 PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >ADN34131.1 tRNA pseudouridine synthase family protein [Cucumis melo subsp. melo] >KAA0064620.1 tRNA pseudouridine synthase A [Cucumis melo var. makuwa] >TYK19972.1 tRNA pseudouridine synthase A [Cucumis melo var. makuwa])

HSP 1 Score: 860.9 bits (2223), Expect = 5.3e-246
Identity = 439/491 (89.41%), Postives = 456/491 (92.87%), Query Frame = 0


Query: 1   MSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 60
           MSSTTTTSDDEDVN NSAAPKKQRYKRRK+AIFFAYCGVGYQGMQKNPGAKTIEADLEEA
Sbjct: 28  MSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 87

Query: 61  LYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 120
           LYLAGAVPE DRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI
Sbjct: 88  LYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 147

Query: 121 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECS 180
           RIFGYKRVTASFNAKKFCDRRRYVYLIPVFAL+PN HRDRESVKASLGSDNELVKCLECS
Sbjct: 148 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECS 207

Query: 181 ERGRKVEGLMGKRNFEAKTVITESEVSSNTGIAVEVSE----GALGDNTPASDSNKQTSV 240
           ERGRKVEGLMGKRNFE KT ITES++SSN   A+EVS+    G L D+   SDSNKQTSV
Sbjct: 208 ERGRKVEGLMGKRNFEVKTAITESDISSNIVNAIEVSDVQANGVLEDSATTSDSNKQTSV 267

Query: 241 LVENGDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARF 300
           LVEN  TEL + SEG  K E +E SGLGNDS+ VVSKESV+G EKPSGSFKYGEEEKARF
Sbjct: 268 LVENTATELTVASEGIAKSESSEMSGLGNDSEIVVSKESVNGKEKPSGSFKYGEEEKARF 327

Query: 301 NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLH 360
           NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDA+TTVVVEGIEFVKCEVVGQSFMLH
Sbjct: 328 NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLH 387

Query: 361 QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESH 420
           QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYL+ECLFTSYNQKWK++H
Sbjct: 388 QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTH 447

Query: 421 EEVSMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLRVADEVADKAN 480
           EEVSMKAYEEVAEDFKMKQIYSHIASTEHK+GAVALWLHSLNHRNYPDLRV DEVA  A 
Sbjct: 448 EEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAK 507

Query: 481 DTCVGMETPAE 488
           +T V MET AE
Sbjct: 508 NTSVDMETQAE 518

[Close]

BLAST of Moc11g00840 vs. NCBI nr
Match: XP_004145528.1 (tRNA pseudouridine synthase A [Cucumis sativus] >KGN55475.1 hypothetical protein Csa_012098 [Cucumis sativus])

HSP 1 Score: 854.7 bits (2207), Expect = 3.8e-244
Identity = 435/491 (88.59%), Postives = 455/491 (92.67%), Query Frame = 0


Query: 1   MSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 60
           MSSTTTTSDDEDVN NSAAPKKQRYKRRK+AIFFAYCGVGYQGMQKNPGAKTIEADLEEA
Sbjct: 28  MSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 87

Query: 61  LYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 120
           LYLAGAVPE DRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI
Sbjct: 88  LYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 147

Query: 121 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECS 180
           RIFGYKRVTASFNAKKFCDRRRYVYLIPVFAL+PN HRDRESVKASLGSDNELVKCLECS
Sbjct: 148 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECS 207

Query: 181 ERGRKVEGLMGKRNFEAKTVITESEVSSNTGIAVEVSE----GALGDNTPASDSNKQTSV 240
           ERGRKVEGL+GKRNFE KT ITES++SSNT  A+EVS+    G L D+   SDSNKQTSV
Sbjct: 208 ERGRKVEGLIGKRNFEVKTAITESDISSNTVNAIEVSDVQANGVLEDSATTSDSNKQTSV 267

Query: 241 LVENGDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARF 300
           LVEN  TELN+GSEGT K E +E SGLG+DS+ VVSKESV+G EK SGSFKYGEEEKARF
Sbjct: 268 LVENTATELNVGSEGTAKSESSEMSGLGSDSEIVVSKESVNGKEKSSGSFKYGEEEKARF 327

Query: 301 NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLH 360
           NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDA+  VVVEGIEFVKCEVVGQSFMLH
Sbjct: 328 NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASAIVVVEGIEFVKCEVVGQSFMLH 387

Query: 361 QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESH 420
           QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYL+ECLFTSYNQKWK++H
Sbjct: 388 QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTH 447

Query: 421 EEVSMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLRVADEVADKAN 480
           EEVSMKAYEE AEDFKMKQIYSHIASTEHK+GAVALWLHSLNHRNYPDLRV DEVA  A 
Sbjct: 448 EEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAK 507

Query: 481 DTCVGMETPAE 488
           +  + MET AE
Sbjct: 508 NASLDMETQAE 518

[Close]

BLAST of Moc11g00840 vs. NCBI nr
Match: XP_022975443.1 (tRNA pseudouridine synthase A [Cucurbita maxima])

HSP 1 Score: 854.4 bits (2206), Expect = 4.9e-244
Identity = 435/491 (88.59%), Postives = 454/491 (92.46%), Query Frame = 0


Query: 1   MSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 60
           MSSTTTTSDDEDVN NSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEA
Sbjct: 28  MSSTTTTSDDEDVNSNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 87

Query: 61  LYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 120
           LYLAGAVPE DRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI
Sbjct: 88  LYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 147

Query: 121 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECS 180
           RIFGYKRVTASFNAKKFCDRRRYVYLIPVFAL+PN HRDRESVKASLGS NELVKCLECS
Sbjct: 148 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSGNELVKCLECS 207

Query: 181 ERGRKVEGLMGKRNFEAKTVITESEVSSNTGIAVEVSE----GALGDNTPASDSNKQTSV 240
           ERGRKVEGLMGKRNFE KT I+ES++S N G A+EVS+    GAL DN  ASDS K+TSV
Sbjct: 208 ERGRKVEGLMGKRNFEVKTAISESDISPNFGNAIEVSDVQANGALEDNATASDSIKETSV 267

Query: 241 LVENGDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARF 300
           LVEN  TELN+GSE   K E +E SGLGNDS+ VVSK+SV+ NEKPSGSFKYGEEEKARF
Sbjct: 268 LVENSGTELNVGSEAIAKSEGSEMSGLGNDSENVVSKDSVNRNEKPSGSFKYGEEEKARF 327

Query: 301 NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLH 360
           NRILK YVGTHNFHNFTTRTKAEDPAARRFI+SFDAA TVVVEG+EFVKCEVVGQSFMLH
Sbjct: 328 NRILKKYVGTHNFHNFTTRTKAEDPAARRFIISFDAAITVVVEGVEFVKCEVVGQSFMLH 387

Query: 361 QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESH 420
           QIRKMMGLAVAIMRNCAPESLIEKALQ+DVNINVPTAPEVGLYL+ECLFTSYNQKWK+SH
Sbjct: 388 QIRKMMGLAVAIMRNCAPESLIEKALQQDVNINVPTAPEVGLYLDECLFTSYNQKWKDSH 447

Query: 421 EEVSMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLRVADEVADKAN 480
           EEVSMKAYEEVAEDFKMK IYSHI STEHKEGAVALWLHSLNHRNYPDLRVADE AD A 
Sbjct: 448 EEVSMKAYEEVAEDFKMKHIYSHIGSTEHKEGAVALWLHSLNHRNYPDLRVADEAADNAK 507

Query: 481 DTCVGMETPAE 488
           +T V +ET AE
Sbjct: 508 NTSVDIETQAE 518

[Close]

BLAST of Moc11g00840 vs. NCBI nr
Match: XP_038899088.1 (tRNA pseudouridine synthase A [Benincasa hispida])

HSP 1 Score: 854.0 bits (2205), Expect = 6.4e-244
Identity = 437/491 (89.00%), Postives = 455/491 (92.67%), Query Frame = 0


Query: 1   MSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 60
           MSSTTTTSDDEDVN +SAAPKKQRYKRRK+AIFFAYCGVGYQGMQKNPGAKTIEADLEEA
Sbjct: 28  MSSTTTTSDDEDVNSSSAAPKKQRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 87

Query: 61  LYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 120
           LYLAGAVPE DRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI
Sbjct: 88  LYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 147

Query: 121 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECS 180
           RIFGYKRVTASFNAKKFCDRRRYVYLIPVFAL+PN HRDRESVKASLGSDNELVKCLECS
Sbjct: 148 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECS 207

Query: 181 ERGRKVEGLMGKRNFEAKTVITESEVSSNTGIAVEVSE----GALGDNTPASDSNKQTSV 240
           ERGRKVEGLMGKRNFE KT I ES +SSNTG A+EVS+      L DN   SDSNKQ+SV
Sbjct: 208 ERGRKVEGLMGKRNFEVKTAIIESHISSNTGNAIEVSDVQENRVLEDNATISDSNKQSSV 267

Query: 241 LVENGDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARF 300
           LVEN  TELN+ SE T K   +E SGLGNDS+ VVSKESV+GNEKPSGSFKY EEEKARF
Sbjct: 268 LVENSCTELNVESESTAKSVGSEMSGLGNDSEIVVSKESVNGNEKPSGSFKYCEEEKARF 327

Query: 301 NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLH 360
           NRILK+YVGTHNFHNFTTRTKAEDPAARRFIVSFDA+TTVVVEGIEFVKCEVVGQSFMLH
Sbjct: 328 NRILKNYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLH 387

Query: 361 QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESH 420
           QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYL+ECLFTSYNQKWK+SH
Sbjct: 388 QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDSH 447

Query: 421 EEVSMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLRVADEVADKAN 480
           EEVSMKAYEEVAEDFKMKQIYSHIASTEHK+GAVALWLHSLNHRNYPDLR  DEVA+ A 
Sbjct: 448 EEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLRAVDEVAEIAM 507

Query: 481 DTCVGMETPAE 488
           +T V MET AE
Sbjct: 508 NTSVAMETQAE 518

[Close]

BLAST of Moc11g00840 vs. ExPASy Swiss-Prot
Match: Q59S63 (tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PUS1 PE=3 SV=2)

HSP 1 Score: 211.5 bits (537), Expect = 2.2e-53
Identity = 144/444 (32.43%), Postives = 225/444 (50.68%), Query Frame = 0


Query: 10  DEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGM--QKNPGAKTIEADLEEALYLAGAV 69
           DE+  P   AP+K    +RK+A+   YCG GY GM  Q +P  KTIE D+ +A+  AGA+
Sbjct: 134 DENGQPIPKAPRK---PKRKVAVMLGYCGTGYNGMQVQNDPNVKTIEKDIYDAMATAGAI 193

Query: 70  PEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKR 129
              +    K+  + R+ARTDKGV A G V+S +  I+ P  ++++N  L  QIRI+G +R
Sbjct: 194 SAENAVDLKKSGFQRAARTDKGVHAAGNVISLKMIIEDPEIINKINDLLPKQIRIWGIQR 253

Query: 130 VTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECSERGRKVE 189
            T  F+ +K C  R Y YL+P F+L P   +   SV + L  + +L +  +  E  +  E
Sbjct: 254 TTKGFDCRKCCSSRIYEYLLPTFSLLPPKPK---SVLSELVKEKKL-ENPDLFEDDQ--E 313

Query: 190 GLMGKRNFEAKTV---ITESEVSSNTGIAVEVSEGALGDNTPASDSNKQTSVLVENGDTE 249
           G+    N ++K +   IT+ ++ S T    E  +         +D         E   +E
Sbjct: 314 GIDWWENVKSKILASGITQEQIDSITSSYDEQQQQQQQQQQQQADEE-------ERDISE 373

Query: 250 LNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARFNRILKHYV 309
           L+                    +K +   ++++   +   S++        F  ++K Y 
Sbjct: 374 LSF-------------------TKLIKQIKTIENQSR--RSYRISSSRLQHFREVMKQYE 433

Query: 310 GTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGL 369
           GTHNFHNFT     +D +A RF++    +   V+EG E++  ++ GQSFMLHQIRKM+ +
Sbjct: 434 GTHNFHNFTVGKPFKDTSANRFMIKTIVSDPFVIEGTEWISIKIHGQSFMLHQIRKMIAM 493

Query: 370 AVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQK-WKESHEEVSMKA 429
           A  ++R   P  +I    Q    IN+P AP +GL LE  +F  YN K  K  +E +    
Sbjct: 494 AALVVRLSLPCGIINNFFQ-STKINIPKAPALGLLLENPVFDGYNIKLTKSDYEPIDFTK 539

Query: 430 YEEVAEDFKMKQIYSHIASTEHKE 448
           +++   +FKMK IY  I + E KE
Sbjct: 554 FDKEMNEFKMKYIYDKIYAEESKE 539

[Close]

BLAST of Moc11g00840 vs. ExPASy Swiss-Prot
Match: Q4KM92 (tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2)

HSP 1 Score: 207.2 bits (526), Expect = 4.1e-52
Identity = 153/479 (31.94%), Postives = 212/479 (44.26%), Query Frame = 0


Query: 11  EDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGA---KTIEADLEEALYLAGAV 70
           ED  P    PK      RKI +  AY G GY GMQ+N G+   +TIE DL  AL  AG +
Sbjct: 69  EDEEPPRKLPK------RKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCI 128

Query: 71  PEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKR 130
           PE+     ++  + R ARTDKGVSA GQVVS + ++     +D++NS+L   IRI G KR
Sbjct: 129 PENHGTDMRKMSFQRCARTDKGVSAAGQVVSLKVWL-IDDILDKINSHLPSHIRILGLKR 188

Query: 131 VTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECSERGRKVE 190
           VT  FN+K  CD R Y Y++P FA    +H+DR+                          
Sbjct: 189 VTGGFNSKNKCDARTYCYMLPTFAF---AHKDRD-------------------------- 248

Query: 191 GLMGKRNFEAKTVITESEVSSNTGIAVEVSEGALGDNTPASDSNKQTSVLVENGDTELNL 250
                                                                       
Sbjct: 249 ------------------------------------------------------------ 308

Query: 251 GSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARFNRILKHYVGTH 310
                                  V  E          S++   E   + NR+L  Y GTH
Sbjct: 309 -----------------------VQDE----------SYRLSAETLQQVNRLLGCYKGTH 368

Query: 311 NFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVA 370
           NFHNFT++    +P+ARR+I+        V EG+EF   +V GQSFM+HQIRKM+GL VA
Sbjct: 369 NFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVA 417

Query: 371 IMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKES--HEEVSMKAYE 430
           I++  APES++E++  ++  ++VP AP +GL LE   F  YNQ++     HE +     E
Sbjct: 429 IVKGYAPESVLERSWGEE-KVDVPKAPGLGLVLERVHFEKYNQRFGSDGLHEPLDWAQEE 417

Query: 431 EVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLRV-ADEVADKANDTCVGME 484
                FK + IY  I STE  E ++A WL++L   N+    + AD+   K   +  G E
Sbjct: 489 GKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNFSGTALGADDTGAKVPSSLEGSE 417

[Close]

BLAST of Moc11g00840 vs. ExPASy Swiss-Prot
Match: Q9WU56 (tRNA pseudouridine synthase A OS=Mus musculus OX=10090 GN=Pus1 PE=1 SV=2)

HSP 1 Score: 203.8 bits (517), Expect = 4.6e-51
Identity = 148/457 (32.39%), Postives = 204/457 (44.64%), Query Frame = 0


Query: 11  EDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGA---KTIEADLEEALYLAGAV 70
           ED  P    PK      RKI +  AY G GY GMQ+N G+   +TIE DL  AL  AG +
Sbjct: 69  EDEEPPRKLPK------RKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCI 128

Query: 71  PEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKR 130
           PE+     ++  + R ARTDKGVSA GQVVS + ++     +D++NS+L   IRI G KR
Sbjct: 129 PENHGTDMRKMSFQRCARTDKGVSAAGQVVSLKVWL-IDDILDKINSHLPSHIRILGLKR 188

Query: 131 VTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECSERGRKVE 190
           VT  FN+K  CD R Y Y++P FA    +H+DR+                          
Sbjct: 189 VTGGFNSKNKCDARTYCYMLPTFAF---AHKDRD-------------------------- 248

Query: 191 GLMGKRNFEAKTVITESEVSSNTGIAVEVSEGALGDNTPASDSNKQTSVLVENGDTELNL 250
                                                                       
Sbjct: 249 ------------------------------------------------------------ 308

Query: 251 GSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARFNRILKHYVGTH 310
                                  V  E          S++   E   + NR+L  Y GTH
Sbjct: 309 -----------------------VQDE----------SYRLSAETLQQVNRLLACYKGTH 368

Query: 311 NFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVA 370
           NFHNFT++    +P+ARR+I+        V EG+EF   +V GQSFM+HQIRKM+GL VA
Sbjct: 369 NFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVA 395

Query: 371 IMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKES--HEEVSMKAYE 430
           I++  APES++E++  ++  ++VP AP +GL LE   F  YNQ++     HE +     E
Sbjct: 429 IVKGYAPESVLERSWGEE-KVDVPKAPGLGLVLERVHFEKYNQRFGGDGLHEPLDWTQEE 395

Query: 431 EVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNY 463
                FK + IY  I STE  E ++A WL++L   N+
Sbjct: 489 GKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNF 395

[Close]

BLAST of Moc11g00840 vs. ExPASy Swiss-Prot
Match: Q6CC39 (tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.7e-50
Identity = 137/427 (32.08%), Postives = 212/427 (49.65%), Query Frame = 0


Query: 24  RYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYLAGAVPEHDRGLSKRYDWARS 83
           R  +RK+A    YCG GY GMQ NP  KTIE D+ +A   AGA+ +++    K+  + R+
Sbjct: 81  RQPKRKVACMIGYCGTGYHGMQLNPPQKTIEGDIFQAFVKAGAISQNNADDPKKSAFMRA 140

Query: 84  ARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRY 143
           ARTDKGV A G V+S +  I+    V+++NS+L  Q+R++G  R   +F  +K C  R Y
Sbjct: 141 ARTDKGVHAAGNVISLKMIIEDENIVEKINSHLPEQLRVWGVSRTNKAFECRKLCSSRVY 200

Query: 144 VYLIPVFAL---NPNSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEAKTV 203
            YL+P ++     P +    + +K     D E                  GK+ +E+   
Sbjct: 201 EYLMPTYSFLNPRPGTVMSEKLLKDGTSPDEE------------------GKKYWESVAA 260

Query: 204 ITESEVSSNTGIAVEVSEGALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKCECNET 263
             ES+     G++               D   + + + E    E    +E  VK +    
Sbjct: 261 DLESQ-----GVSY--------------DEWMKRACIDEIKGEETKEVAESEVKTDSK-- 320

Query: 264 SGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTKAED 323
                D+ T+   ++V+   +    F+   E  A+   ILK Y GTHNFHNFT     +D
Sbjct: 321 ----TDAATLEKIKAVERRHRE--EFRISGERLAKIREILKIYEGTHNFHNFTLGKAFKD 380

Query: 324 PAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGL-AVAIMRNCAPESLIE 383
           P+A R + S   +   +++G E+V  ++ GQSFMLHQIRKM+ + A+++  N  P+ LI 
Sbjct: 381 PSAMRTMKSLTCSDPFLIDGTEWVSIKIHGQSFMLHQIRKMISMVALSVRCNADPQKLIP 440

Query: 384 KALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKE--SHEEVSMKAYEEVAEDFKMKQIY 443
           +  +K   IN+P AP +GL LE  ++ SYN+K +     E V    + +  E FK K IY
Sbjct: 441 QTFEK-ARINIPKAPALGLLLERPVYDSYNKKLQGEFGREGVHFDNWNDQIEAFKHKFIY 461

Query: 444 SHIASTE 445
             I + E
Sbjct: 501 DKIYAEE 461

[Close]

BLAST of Moc11g00840 vs. ExPASy Swiss-Prot
Match: Q9Y606 (tRNA pseudouridine synthase A OS=Homo sapiens OX=9606 GN=PUS1 PE=1 SV=3)

HSP 1 Score: 199.1 bits (505), Expect = 1.1e-49
Identity = 141/447 (31.54%), Postives = 200/447 (44.74%), Query Frame = 0


Query: 21  KKQRYKRRKIAIFFAYCGVGYQGMQKNPGA---KTIEADLEEALYLAGAVPEHDRGLSKR 80
           ++++  +RKI +  AY G GY GMQ+N G+   KTIE DL  AL  +G +PE+     ++
Sbjct: 77  RREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRK 136

Query: 81  YDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIFGYKRVTASFNAKKF 140
             + R ARTDKGVSA GQVVS + ++     ++++NS+L   IRI G KRVT  FN+K  
Sbjct: 137 MSFQRCARTDKGVSAAGQVVSLKVWL-IDDILEKINSHLPSHIRILGLKRVTGGFNSKNR 196

Query: 141 CDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECSERGRKVEGLMGKRNFEA 200
           CD R Y YL+P FA    +H+DR+                                    
Sbjct: 197 CDARTYCYLLPTFAF---AHKDRD------------------------------------ 256

Query: 201 KTVITESEVSSNTGIAVEVSEGALGDNTPASDSNKQTSVLVENGDTELNLGSEGTVKCEC 260
                                  + D T                                
Sbjct: 257 -----------------------VQDET-------------------------------- 316

Query: 261 NETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARFNRILKHYVGTHNFHNFTTRTK 320
                                       ++   E   + NR+L  Y GTHNFHNFT++  
Sbjct: 317 ----------------------------YRLSAETLQQVNRLLACYKGTHNFHNFTSQKG 376

Query: 321 AEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRKMMGLAVAIMRNCAPESL 380
            +DP+A R+I+        V EG+EF    V GQSFM+HQIRKM+GL VAI++  APES+
Sbjct: 377 PQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESV 399

Query: 381 IEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKES--HEEVSMKAYEEVAEDFKMKQ 440
           +E++   +  ++VP AP +GL LE   F  YNQ++     HE +     E     FK + 
Sbjct: 437 LERSWGTE-KVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEH 399

Query: 441 IYSHIASTEHKEGAVALWLHSLNHRNY 463
           IY  I  TE  E ++A WL +L   N+
Sbjct: 497 IYPTIIGTERDERSMAQWLSTLPIHNF 399

[Close]

BLAST of Moc11g00840 vs. ExPASy TrEMBL
Match: A0A6J1BUF3 (tRNA pseudouridine synthase A, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111005637 PE=3 SV=1)

HSP 1 Score: 969.5 bits (2505), Expect = 5.1e-279
Identity = 488/488 (100.00%), Postives = 488/488 (100.00%), Query Frame = 0


Query: 1   MSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 60
           MSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEA
Sbjct: 29  MSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 88

Query: 61  LYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 120
           LYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI
Sbjct: 89  LYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 148

Query: 121 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECS 180
           RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECS
Sbjct: 149 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECS 208

Query: 181 ERGRKVEGLMGKRNFEAKTVITESEVSSNTGIAVEVSEGALGDNTPASDSNKQTSVLVEN 240
           ERGRKVEGLMGKRNFEAKTVITESEVSSNTGIAVEVSEGALGDNTPASDSNKQTSVLVEN
Sbjct: 209 ERGRKVEGLMGKRNFEAKTVITESEVSSNTGIAVEVSEGALGDNTPASDSNKQTSVLVEN 268

Query: 241 GDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARFNRIL 300
           GDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARFNRIL
Sbjct: 269 GDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARFNRIL 328

Query: 301 KHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRK 360
           KHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRK
Sbjct: 329 KHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQIRK 388

Query: 361 MMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESHEEVS 420
           MMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESHEEVS
Sbjct: 389 MMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESHEEVS 448

Query: 421 MKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLRVADEVADKANDTCV 480
           MKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLRVADEVADKANDTCV
Sbjct: 449 MKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLRVADEVADKANDTCV 508

Query: 481 GMETPAEI 489
           GMETPAEI
Sbjct: 509 GMETPAEI 516

[Close]

BLAST of Moc11g00840 vs. ExPASy TrEMBL
Match: A0A1S3BV07 (tRNA pseudouridine synthase A, mitochondrial-like OS=Cucumis melo OX=3656 GN=LOC103493789 PE=3 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 2.5e-246
Identity = 439/491 (89.41%), Postives = 456/491 (92.87%), Query Frame = 0


Query: 1   MSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 60
           MSSTTTTSDDEDVN NSAAPKKQRYKRRK+AIFFAYCGVGYQGMQKNPGAKTIEADLEEA
Sbjct: 28  MSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 87

Query: 61  LYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 120
           LYLAGAVPE DRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI
Sbjct: 88  LYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 147

Query: 121 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECS 180
           RIFGYKRVTASFNAKKFCDRRRYVYLIPVFAL+PN HRDRESVKASLGSDNELVKCLECS
Sbjct: 148 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECS 207

Query: 181 ERGRKVEGLMGKRNFEAKTVITESEVSSNTGIAVEVSE----GALGDNTPASDSNKQTSV 240
           ERGRKVEGLMGKRNFE KT ITES++SSN   A+EVS+    G L D+   SDSNKQTSV
Sbjct: 208 ERGRKVEGLMGKRNFEVKTAITESDISSNIVNAIEVSDVQANGVLEDSATTSDSNKQTSV 267

Query: 241 LVENGDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARF 300
           LVEN  TEL + SEG  K E +E SGLGNDS+ VVSKESV+G EKPSGSFKYGEEEKARF
Sbjct: 268 LVENTATELTVASEGIAKSESSEMSGLGNDSEIVVSKESVNGKEKPSGSFKYGEEEKARF 327

Query: 301 NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLH 360
           NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDA+TTVVVEGIEFVKCEVVGQSFMLH
Sbjct: 328 NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLH 387

Query: 361 QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESH 420
           QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYL+ECLFTSYNQKWK++H
Sbjct: 388 QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTH 447

Query: 421 EEVSMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLRVADEVADKAN 480
           EEVSMKAYEEVAEDFKMKQIYSHIASTEHK+GAVALWLHSLNHRNYPDLRV DEVA  A 
Sbjct: 448 EEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAK 507

Query: 481 DTCVGMETPAE 488
           +T V MET AE
Sbjct: 508 NTSVDMETQAE 518

[Close]

BLAST of Moc11g00840 vs. ExPASy TrEMBL
Match: A0A5A7VBT0 (tRNA pseudouridine synthase A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00860 PE=3 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 2.5e-246
Identity = 439/491 (89.41%), Postives = 456/491 (92.87%), Query Frame = 0


Query: 1   MSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 60
           MSSTTTTSDDEDVN NSAAPKKQRYKRRK+AIFFAYCGVGYQGMQKNPGAKTIEADLEEA
Sbjct: 28  MSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 87

Query: 61  LYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 120
           LYLAGAVPE DRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI
Sbjct: 88  LYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 147

Query: 121 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECS 180
           RIFGYKRVTASFNAKKFCDRRRYVYLIPVFAL+PN HRDRESVKASLGSDNELVKCLECS
Sbjct: 148 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECS 207

Query: 181 ERGRKVEGLMGKRNFEAKTVITESEVSSNTGIAVEVSE----GALGDNTPASDSNKQTSV 240
           ERGRKVEGLMGKRNFE KT ITES++SSN   A+EVS+    G L D+   SDSNKQTSV
Sbjct: 208 ERGRKVEGLMGKRNFEVKTAITESDISSNIVNAIEVSDVQANGVLEDSATTSDSNKQTSV 267

Query: 241 LVENGDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARF 300
           LVEN  TEL + SEG  K E +E SGLGNDS+ VVSKESV+G EKPSGSFKYGEEEKARF
Sbjct: 268 LVENTATELTVASEGIAKSESSEMSGLGNDSEIVVSKESVNGKEKPSGSFKYGEEEKARF 327

Query: 301 NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLH 360
           NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDA+TTVVVEGIEFVKCEVVGQSFMLH
Sbjct: 328 NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLH 387

Query: 361 QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESH 420
           QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYL+ECLFTSYNQKWK++H
Sbjct: 388 QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTH 447

Query: 421 EEVSMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLRVADEVADKAN 480
           EEVSMKAYEEVAEDFKMKQIYSHIASTEHK+GAVALWLHSLNHRNYPDLRV DEVA  A 
Sbjct: 448 EEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAK 507

Query: 481 DTCVGMETPAE 488
           +T V MET AE
Sbjct: 508 NTSVDMETQAE 518

[Close]

BLAST of Moc11g00840 vs. ExPASy TrEMBL
Match: E5GCD2 (tRNA pseudouridine synthase family protein OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 2.5e-246
Identity = 439/491 (89.41%), Postives = 456/491 (92.87%), Query Frame = 0


Query: 1   MSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 60
           MSSTTTTSDDEDVN NSAAPKKQRYKRRK+AIFFAYCGVGYQGMQKNPGAKTIEADLEEA
Sbjct: 28  MSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 87

Query: 61  LYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 120
           LYLAGAVPE DRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI
Sbjct: 88  LYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 147

Query: 121 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECS 180
           RIFGYKRVTASFNAKKFCDRRRYVYLIPVFAL+PN HRDRESVKASLGSDNELVKCLECS
Sbjct: 148 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECS 207

Query: 181 ERGRKVEGLMGKRNFEAKTVITESEVSSNTGIAVEVSE----GALGDNTPASDSNKQTSV 240
           ERGRKVEGLMGKRNFE KT ITES++SSN   A+EVS+    G L D+   SDSNKQTSV
Sbjct: 208 ERGRKVEGLMGKRNFEVKTAITESDISSNIVNAIEVSDVQANGVLEDSATTSDSNKQTSV 267

Query: 241 LVENGDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARF 300
           LVEN  TEL + SEG  K E +E SGLGNDS+ VVSKESV+G EKPSGSFKYGEEEKARF
Sbjct: 268 LVENTATELTVASEGIAKSESSEMSGLGNDSEIVVSKESVNGKEKPSGSFKYGEEEKARF 327

Query: 301 NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLH 360
           NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDA+TTVVVEGIEFVKCEVVGQSFMLH
Sbjct: 328 NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASTTVVVEGIEFVKCEVVGQSFMLH 387

Query: 361 QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESH 420
           QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYL+ECLFTSYNQKWK++H
Sbjct: 388 QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTH 447

Query: 421 EEVSMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLRVADEVADKAN 480
           EEVSMKAYEEVAEDFKMKQIYSHIASTEHK+GAVALWLHSLNHRNYPDLRV DEVA  A 
Sbjct: 448 EEVSMKAYEEVAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAK 507

Query: 481 DTCVGMETPAE 488
           +T V MET AE
Sbjct: 508 NTSVDMETQAE 518

[Close]

BLAST of Moc11g00840 vs. ExPASy TrEMBL
Match: A0A0A0L0J9 (PseudoU_synth_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G652870 PE=3 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 1.8e-244
Identity = 435/491 (88.59%), Postives = 455/491 (92.67%), Query Frame = 0


Query: 1   MSSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 60
           MSSTTTTSDDEDVN NSAAPKKQRYKRRK+AIFFAYCGVGYQGMQKNPGAKTIEADLEEA
Sbjct: 28  MSSTTTTSDDEDVNSNSAAPKKQRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEADLEEA 87

Query: 61  LYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 120
           LYLAGAVPE DRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI
Sbjct: 88  LYLAGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQI 147

Query: 121 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECS 180
           RIFGYKRVTASFNAKKFCDRRRYVYLIPVFAL+PN HRDRESVKASLGSDNELVKCLECS
Sbjct: 148 RIFGYKRVTASFNAKKFCDRRRYVYLIPVFALDPNCHRDRESVKASLGSDNELVKCLECS 207

Query: 181 ERGRKVEGLMGKRNFEAKTVITESEVSSNTGIAVEVSE----GALGDNTPASDSNKQTSV 240
           ERGRKVEGL+GKRNFE KT ITES++SSNT  A+EVS+    G L D+   SDSNKQTSV
Sbjct: 208 ERGRKVEGLIGKRNFEVKTAITESDISSNTVNAIEVSDVQANGVLEDSATTSDSNKQTSV 267

Query: 241 LVENGDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARF 300
           LVEN  TELN+GSEGT K E +E SGLG+DS+ VVSKESV+G EK SGSFKYGEEEKARF
Sbjct: 268 LVENTATELNVGSEGTAKSESSEMSGLGSDSEIVVSKESVNGKEKSSGSFKYGEEEKARF 327

Query: 301 NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLH 360
           NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDA+  VVVEGIEFVKCEVVGQSFMLH
Sbjct: 328 NRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDASAIVVVEGIEFVKCEVVGQSFMLH 387

Query: 361 QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESH 420
           QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYL+ECLFTSYNQKWK++H
Sbjct: 388 QIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLDECLFTSYNQKWKDTH 447

Query: 421 EEVSMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLRVADEVADKAN 480
           EEVSMKAYEE AEDFKMKQIYSHIASTEHK+GAVALWLHSLNHRNYPDLRV DEVA  A 
Sbjct: 448 EEVSMKAYEEAAEDFKMKQIYSHIASTEHKDGAVALWLHSLNHRNYPDLRVIDEVAKIAK 507

Query: 481 DTCVGMETPAE 488
           +  + MET AE
Sbjct: 508 NASLDMETQAE 518

[Close]

BLAST of Moc11g00840 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 530.0 bits (1364), Expect = 2.0e-150
Identity = 283/478 (59.21%), Postives = 351/478 (73.43%), Query Frame = 0


Query: 1   MSSTTTTSDDEDV---NPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADL 60
           +S+T   +D   V     N A  +  +Y+RRK+AI FA+CGVGYQGMQKNPGAKTIE +L
Sbjct: 28  ISTTGQETDSSAVTTGGSNVAERRAPKYRRRKVAIVFAFCGVGYQGMQKNPGAKTIEGEL 87

Query: 61  EEALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLS 120
           EEAL+ AGAVP+ DR   + Y+WARSARTDKGVSAVGQVVSGRFY+DPPGFV+RLNS L 
Sbjct: 88  EEALFHAGAVPDADRNKPRNYEWARSARTDKGVSAVGQVVSGRFYVDPPGFVERLNSKLP 147

Query: 121 PQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCL 180
            QIR+FGYKRV  SF++KKFCDRRRYVYLIPVFAL+P  H + E V+  LG   E VKC+
Sbjct: 148 DQIRVFGYKRVAPSFSSKKFCDRRRYVYLIPVFALDPCVHGEAEMVRTDLG--YEYVKCV 207

Query: 181 ECSERGRKVE-GLMGKRN--FEAKTVITESEVSSNT--GIAVEVSEGALGDNTPASDSNK 240
           ECSE+G K+  G+MGK     + K++  +S++SS+T   I  +V    L  + P++++N 
Sbjct: 208 ECSEKGYKIPLGVMGKDTDCCDTKSLEIQSDISSSTCDAIGADVKCETLSSSVPSAENNL 267

Query: 241 QTSVLVENGDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGN----EKPSGSFKY 300
            + VL     + L+  +EG              +S T+   E  +G      K    F Y
Sbjct: 268 NSEVLDGADVSALSSKAEGM------------EESNTLAKVEMNNGEGEDMTKSESKFCY 327

Query: 301 GEEEKARFNRILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEV 360
           GE+EK RFNRIL +YVG+HNFHNFTTRTKA DPAA R+I+SF+A T + ++G +FVKCEV
Sbjct: 328 GEKEKERFNRILSYYVGSHNFHNFTTRTKAADPAANRYILSFNANTVINLDGKDFVKCEV 387

Query: 361 VGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSY 420
           VGQSFMLHQIRKM+GLAVAIMRN APESLIE + +KDV INVP APEVGLYL+EC FTSY
Sbjct: 388 VGQSFMLHQIRKMIGLAVAIMRNYAPESLIEASFKKDVRINVPMAPEVGLYLDECFFTSY 447

Query: 421 NQKWKESHEEVSMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLR 467
           N+++K SHEEVSM+ Y+EVAE+FK K +YSHI S E K+GAVA+WLHSLN RNYPDLR
Sbjct: 448 NKRFKGSHEEVSMEEYKEVAEEFKWKYVYSHIGSAEEKDGAVAIWLHSLNQRNYPDLR 491

[Close]

BLAST of Moc11g00840 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 519.6 bits (1337), Expect = 2.7e-147
Identity = 282/468 (60.26%), Postives = 340/468 (72.65%), Query Frame = 0


Query: 4   TTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYL 63
           TTTT ++ D++ +    + Q     K+AI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ 
Sbjct: 3   TTTTDEEIDLSCDGGVTEPQ-----KVAIIFAFCGVGYQGMQKNPGAKTIEGELEEALFH 62

Query: 64  AGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIF 123
           AGAVPE  RG  K YD+ARSARTDKGVSAVGQVVSGRF +DP GFV+RLNSNL  QIRIF
Sbjct: 63  AGAVPESIRGKPKLYDFARSARTDKGVSAVGQVVSGRFIVDPLGFVNRLNSNLPNQIRIF 122

Query: 124 GYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECSERG 183
           GYK VT SF++KKFCDRRRYVYL+PVFAL+P SHRDRE+V ASLG   E VKC ECSERG
Sbjct: 123 GYKHVTPSFSSKKFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERG 182

Query: 184 RKV-EGLMGK---RNFEAKTVITESEVSSNTGIAV--EVSEGALGDNTPASDSNKQTSVL 243
           RK+  GL+GK    NF  K++  +S++SSN   A+  ++   AL  N     S       
Sbjct: 183 RKIPPGLVGKWKGTNFGTKSLDFQSDISSNNSSALRSDIKIEALSSNLAGLCS------- 242

Query: 244 VENGDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARFN 303
                 ++ +G      C+ N      N S+T           K    F YGE+EK RF+
Sbjct: 243 -----VDVEVGRIQEDSCKLNT-----NSSET-----------KVKSKFCYGEKEKERFS 302

Query: 304 RILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQ 363
           RIL  YVG++NFHNFTTRTKA+DP A R I+SF A T + ++GI+F+KCEV+G+SFMLHQ
Sbjct: 303 RILSCYVGSYNFHNFTTRTKADDPTANRQIISFTANTVINLDGIDFIKCEVLGKSFMLHQ 362

Query: 364 IRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYLEECLFTSYNQKWKESHE 423
           IRKMMGLAVAIMRNCA ESLI+ A  KDVNI VP APEVGLYL+EC FTSYN+ +++SHE
Sbjct: 363 IRKMMGLAVAIMRNCASESLIQSAFSKDVNITVPMAPEVGLYLDECFFTSYNRNFEDSHE 422

Query: 424 EVSMKAYEEVAEDFKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDL 466
           EVSM+AY+E AE FK+K IYSHI +TE K G +ALWLHSLN+RNYPDL
Sbjct: 423 EVSMEAYKEEAEAFKLKHIYSHIGATERKYGNMALWLHSLNYRNYPDL 437

[Close]

BLAST of Moc11g00840 vs. TAIR 10
Match: AT1G76120.2 (Pseudouridine synthase family protein )

HSP 1 Score: 396.0 bits (1016), Expect = 4.5e-110
Identity = 223/387 (57.62%), Postives = 270/387 (69.77%), Query Frame = 0


Query: 4   TTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEADLEEALYL 63
           TTTT ++ D++ +    + Q     K+AI FA+CGVGYQGMQKNPGAKTIE +LEEAL+ 
Sbjct: 3   TTTTDEEIDLSCDGGVTEPQ-----KVAIIFAFCGVGYQGMQKNPGAKTIEGELEEALFH 62

Query: 64  AGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLSPQIRIF 123
           AGAVPE  RG  K YD+ARSARTDKGVSAVGQVVSGRF +DP GFV+RLNSNL  QIRIF
Sbjct: 63  AGAVPESIRGKPKLYDFARSARTDKGVSAVGQVVSGRFIVDPLGFVNRLNSNLPNQIRIF 122

Query: 124 GYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGSDNELVKCLECSERG 183
           GYK VT SF++KKFCDRRRYVYL+PVFAL+P SHRDRE+V ASLG   E VKC ECSERG
Sbjct: 123 GYKHVTPSFSSKKFCDRRRYVYLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERG 182

Query: 184 RKV-EGLMGK---RNFEAKTVITESEVSSNTGIAV--EVSEGALGDNTPASDSNKQTSVL 243
           RK+  GL+GK    NF  K++  +S++SSN   A+  ++   AL  N     S       
Sbjct: 183 RKIPPGLVGKWKGTNFGTKSLDFQSDISSNNSSALRSDIKIEALSSNLAGLCS------- 242

Query: 244 VENGDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSFKYGEEEKARFN 303
                 ++ +G      C+ N      N S+T           K    F YGE+EK RF+
Sbjct: 243 -----VDVEVGRIQEDSCKLNT-----NSSET-----------KVKSKFCYGEKEKERFS 302

Query: 304 RILKHYVGTHNFHNFTTRTKAEDPAARRFIVSFDAATTVVVEGIEFVKCEVVGQSFMLHQ 363
           RIL  YVG++NFHNFTTRTKA+DP A R I+SF A T + ++GI+F+KCEV+G+SFMLHQ
Sbjct: 303 RILSCYVGSYNFHNFTTRTKADDPTANRQIISFTANTVINLDGIDFIKCEVLGKSFMLHQ 356

Query: 364 IRKMMGLAVAIMRNCAPESLIEKALQK 385
           IRKMMGLAVAIMRNCA ESLI+ A  K
Sbjct: 363 IRKMMGLAVAIMRNCASESLIQSAFSK 356

[Close]

BLAST of Moc11g00840 vs. TAIR 10
Match: AT2G30320.1 (Pseudouridine synthase family protein )

HSP 1 Score: 115.5 bits (288), Expect = 1.2e-25
Identity = 112/415 (26.99%), Postives = 179/415 (43.13%), Query Frame = 0


Query: 2   SSTTTTSDDEDVNPNSAAPKKQRYKRRKIAIFFAYCGVGYQGMQ---KNPGAKTIEADLE 61
           SST+ +    D   N  A K + Y+++K+ I   Y G  Y+G+Q    +P  KTIE +LE
Sbjct: 40  SSTSLSPPSSD---NFLADKWESYRKKKVVIRIGYVGTDYRGLQIQRDDPSIKTIEGELE 99

Query: 62  EALYLAGAVPEHDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYI-------DPPGFV-- 121
            A+Y AG + + + G   +  WARS+RTDKGV ++   +S +  I       DP G V  
Sbjct: 100 VAIYKAGGIRDSNYGDLHKIGWARSSRTDKGVHSLATSISLKMEIPETAWKDDPQGTVLA 159

Query: 122 DRLNSNLSPQIRIFGYKRVTASFNAKKFCDRRRYVYLIPVFALNPNSHRDRESVKASLGS 181
             ++ +L   IR+F        F+ ++ C  R+Y YL+PV  L   +    + +   +  
Sbjct: 160 KCISKHLPENIRVFSVLPSNRRFDPRRECTLRKYSYLLPVDVLGIKNSFTSDEIDYHITD 219

Query: 182 DNELVKCLECSERGRKVEGLMGKRNFEAKTVI---TESEVSSNTGIAVEVSEGALGDNTP 241
            NE++K         + EG     N+  ++     +E ++    G      +     +  
Sbjct: 220 FNEILK---------EFEGEYPFHNYTQRSRYRRKSEQKIKQRNGRPPREPK-----SIK 279

Query: 242 ASDSNKQTSVLVENGDTELNLGSEGTVKCECNETSGLGNDSKTVVSKESVDGNEKPSGSF 301
           AS+S  +    +E G+ E              E    G   + VV+ +S +        +
Sbjct: 280 ASESEFREENHIEIGEEE-------------EEKEVDGESDEHVVTPDSDNSQVYSRAKW 339

Query: 302 KYGEEEKARFNRILKHYVGTHNFHNFTTRT-KAEDPAARRFIVSFDAATTVVVEGIEFVK 361
            Y  +E  + +       G H    F  R+ K E+                   G  FV+
Sbjct: 340 LYEPDETDKIS-------GAHFRKVFRCRSGKLENSL-----------------GFGFVE 399

Query: 362 CEVVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVNINVPTAPEVGLYL 401
             + G+SFMLHQIRKM+G AVA+ R   P  +I  +L K   I +P AP   L L
Sbjct: 400 ISIWGESFMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPLAPSEVLIL 400

[Close]

BLAST of Moc11g00840 vs. TAIR 10
Match: AT1G20290.1 (SWI-SNF-related chromatin binding protein )

HSP 1 Score: 65.9 bits (159), Expect = 1.1e-10
Identity = 33/53 (62.26%), Postives = 38/53 (71.70%), Query Frame = 0


Query: 415 SHEEVSMKAYEEVAED-FKMKQIYSHIASTEHKEGAVALWLHSLNHRNYPDLR 467
           S  EVS+   +E   D FK K +YSHI S E K+GAVA+WLHSLN RNYPDLR
Sbjct: 82  SWREVSVDDDQEQEHDQFKWKHVYSHIGSAEEKDGAVAIWLHSLNQRNYPDLR 134

The following BLAST results are available for this feature:

BLAST of Moc11g00840 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132914.1	1.1e-278	100.00	tRNA pseudouridine synthase A, mitochondrial-like [Momordica charantia]	[more]
XP_008452911.1	5.3e-246	89.41	PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Cucumis melo] >XP_...	[more]
XP_004145528.1	3.8e-244	88.59	tRNA pseudouridine synthase A [Cucumis sativus] >KGN55475.1 hypothetical protein...	[more]
XP_022975443.1	4.9e-244	88.59	tRNA pseudouridine synthase A [Cucurbita maxima]	[more]
XP_038899088.1	6.4e-244	89.00	tRNA pseudouridine synthase A [Benincasa hispida]	[more]

BLAST of Moc11g00840 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q59S63	2.2e-53	32.43	tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876...	[more]
Q4KM92	4.1e-52	31.94	tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2	[more]
Q9WU56	4.6e-51	32.39	tRNA pseudouridine synthase A OS=Mus musculus OX=10090 GN=Pus1 PE=1 SV=2	[more]
Q6CC39	1.7e-50	32.08	tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) O...	[more]
Q9Y606	1.1e-49	31.54	tRNA pseudouridine synthase A OS=Homo sapiens OX=9606 GN=PUS1 PE=1 SV=3	[more]

BLAST of Moc11g00840 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BUF3	5.1e-279	100.00	tRNA pseudouridine synthase A, mitochondrial-like OS=Momordica charantia OX=3673...	[more]
A0A1S3BV07	2.5e-246	89.41	tRNA pseudouridine synthase A, mitochondrial-like OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A5A7VBT0	2.5e-246	89.41	tRNA pseudouridine synthase A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
E5GCD2	2.5e-246	89.41	tRNA pseudouridine synthase family protein OS=Cucumis melo subsp. melo OX=412675...	[more]
A0A0A0L0J9	1.8e-244	88.59	PseudoU_synth_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G65...	[more]

BLAST of Moc11g00840 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20370.1	2.0e-150	59.21	Pseudouridine synthase family protein	[more]
AT1G76120.1	2.7e-147	60.26	Pseudouridine synthase family protein	[more]
AT1G76120.2	4.5e-110	57.62	Pseudouridine synthase family protein	[more]
AT2G30320.1	1.2e-25	26.99	Pseudouridine synthase family protein	[more]
AT1G20290.1	1.1e-10	62.26	SWI-SNF-related chromatin binding protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 300..405 e-value: 2.3E-15 score: 57.0
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 18..138 e-value: 1.5E-28 score: 101.2
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 267..469 e-value: 2.2E-55 score: 189.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 266..287
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR11142:SF24	TRNA PSEUDOURIDINE SYNTHASE	coord: 4..467
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 4..467
IPR041708	Pseudouridine synthase PUS1/ PUS2-like	CDD	cd02568	PseudoU_synth_PUS1_PUS2	coord: 32..405 e-value: 4.52366E-106 score: 314.166
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 26..415

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc11g00840.1	Moc11g00840.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1990481	mRNA pseudouridine synthesis
biological_process	GO:0031119	tRNA pseudouridine synthesis
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
cellular_component	GO:0005634	nucleus
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding