Moc11g00830 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc11g00830
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Alpha-amylase
Location	chr11: 542645 .. 548398 (-)
RNA-Seq Expression	Moc11g00830
Synteny	Moc11g00830

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTACCATTGCATTTCCTGAAACCGACAGTTGCTTAAGGCTTAAGCCACTGCCGAAAAATGGTTAGCGACGGAACATGAACACACTGGCCAAGGTCCGCATCCCACTCTCATTGGATCGGTTTTTGCGTACGTTCTCGAGCCCACGCGTCTGCAGATCAAAACCTTGAAACTTTTGAGACCAATCATTTGCTCGCGCTAATGGTGGACCAGAGAACATCTAATACTTCTTGGTAGCGTTTCTCACTTGTTTATTAAGTAATTTCTTTCATCTTCTAATGGCGTCGAAATGCGGATTTATAATCGCGGATATATATAGCTGTTTGTCCATTGAATACGTGATCGATCACTCTTAAAGCATCTGTCGTAGCCTTTGCGTTTCTCTTCGTAGCTTTTTTTCTTCCGTGCCAAATGTATTGAAGGTGGTTGTTTGTTTATCTTGTTATATTGTCTCTTTCTTGATTTTGTCACTGGATTATATTTCCTCCTAAGTGCAGACTTCGGATTCGCGCCAATTTGAAACTCGTGATTTTTAGTTGATTGATTCGTATTATCGAGGTTGATCGCATTTTTCTTGTTATTAGTTTATACTTATGAAGAAATTGATTTTGGACAGCTTTAAGAAGTGTTAATGTTTAATACAATTTCAGGTTGAGGATTAGTTTCTAATGATGAAGGAGGTTGTTGAATGTTTAGTACCGCTTGCAGAACTAAGCCAGAGACATGAATGGTCCACACTGCATTACTTCCGTAATAGAATTCTAGCTTGTTCTCTTATTTTCATTTTCCTTGTTCGAGTGAGCATACTGAAGTTGAGACTGGAAACTATGAATGAGATTACAGTGTTTGACGCTCTTTTTATCTTTGATTGTGGTGCAGGACATCCCTATTTTCTTCAACTAAGGCGTCAAGGTTTTCACTAGTTTCTACCGAGTTATTATGGGCGATTCTGATAATGTATGCTTTGTATTTAATCCAGATTTACCATAGGATTTTTAATGGATTATTTTTGCACGTGGACAAACAAAAATAAGCTCAATTTGGCCAATTTGTTCCATGATGAATTGTCTGCATCATTATTATATGATTATAGTCATAAATTTAGTAGAACTTACACTGTAATATACAATTTACTAATTTATATTGGACTTCAGGGTTTTGATGACAGCTATGAGCAGACTGATATTGGTACGTTGTTTTCTATTGTTTGTTTTAACATTAAATTCCCCATCATTATATGATCCCGTTATTGTTAAGTGTTTGTCAAAAGATGATAGCTCCGTTGCAAGTGCCTTCAAATTCTAATGAATGAACTGGTGCATATGCATACCCGTAGATGGGCCTAGCTAACCAAGAGCAGTTTGATGATGCTTAAAATTTGACTCATGGACGATAAGTTATTTAAAAAGAAGACATTATATGCTTTTTAAGTATGAGTGCGTTGAGCAATATTCAATTTTATAATAATACTTGCGGTTTGTGGCAGATATATACACAAGTGCTTGAAATTTGAGTTGGTTTTCTGATATGTAGAAAAATTATTTTTTGTGATTAATATCATCTTATCAATTTAAATTATTCTAGTGTTTCTTTTTGGTCACTTATAGGGGCAGCGATACGCAATGGAAGAGAAATCCTTTTCCAGGTAGAGGTTACCATTTTAGCTTTCTGATCTTTCTGTAGAGCAGAACTGCAGGAAGATATTTTGTAGCTTTTAATTGGGGCGAAGTCCTCCCTCCTCCTCCCAAAAGAAAGAAGAAAAGAAAAGAAAAGAAAAGATGTCTGGCTTTTGAAATTCATCCTGGATATTGTATCTTTTTGTGTATCAAATAAATTTAAAGTCGATGTGGGAGGACTGCACCAATGTCAATGTTTTAGATCACATCTCCCTTGAGTCATTTTAAACTCTACAGAGAAATTCAGCACCCATGGTTTCAATTGTAATTTATAGGCATTCAACTGGGAATCTCAAAAATGTGATTGGTGGAGGAATCTAGAGTGTAAAGTTCGCGATATTGCAGAATCTGGATTTACCTCTGCCTGGTTGCCACCTCCAACTCATTCCATCTCTCCTGAAGGTTAAAGGAACTAATTTTCTTGAATCTTGGTTTTAATTTTCCATGTATGAGTGCCATTATAACTTTTGGTGTATTCTTTATTATTTCAGGTTATCTCCCTCAGAACTTATACTCCCTAAACTCTTCATATGGTTCTGAGGACATGCTTAAAGCTTTACTTTAGAAAATGAAGCAGTGCAAGGTTAGAGCAACTGCTGATATAGTCATCAATCACCATGGAATACCATTACCATGGAATGAATTTGCTGTTACCTCATGCACTGGCGGCCTGGTAATCTGGTATTTCTTTGTTATAAATTCCAAGTGAGCCCATAATTATCAGAAAATGTACGATCCTTAGCCTGTGGTATCACGTTATAGGGCAATCGTAGCACTGGGGACAACTTCCATGGGGTTCCAAACATCGATCATAGCCAACGTTTTGTCCGTAGAGATATCATAGAATGGCTGAAGTGGCTACGCGGTAATGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGAGGGTAATCTTTTCCTTCTCTGTTTGTTTCTTTTTTTGCGAAGGGTAGTACTTTTCTTCTATGCAAGAATTTTTTTTTTTTTTTAGTTTTACTCATTGTTTGCATCATCACGGGATTTGTGAGATTGAGGACTGAACTGGCGCTTGGTGTCGGTTTAAAAAAAAAAAAAAACCTTCTGTTTGTTTTCCACAATAATGATAACGTGAGATACATTGAAACGTGTTTGTGGTTTCCTCTCTTTCCCCCATCCTCTAAAAGGAAGCTTTCTTTTTTGAGTAACTCAAGTTTCTGAATATGATAACATCGCTCTTCTTTGTTTCAAATCTTCACTCTCTATCATCTTCTTCACTAATTTCATCTTGCCTTTTCTTTTAAGGAGTTATTCAGTTGGTTCTAATATACATTCGTTTTTTGCTTTTATCTTTGATGTTCAAGCTCAGTTATTCTGCAAAATTTGTGAAAGAATATATTGAAGCAGCAAAGCCGATATTTTCTATTGGAGAGTATTGGGATTCTTGTAATTATAATGGCAATGACTTGGACCATAATCAAGGTAAAACGATGCATTTTGTTCTTTGTATGTATTATGTGATCAATTGGAATCATGTAATATGCAATATACCCAATTGCTGACTTGGACTATGAAAAGGATTTTCTTTTTGATTTCGAATTGTCGATGTTTGTGAATAATGTCCTCTGTTTCTTTCTTTCTTTTTTTTTAATCAATTTTTGTATTCTATAATTATGATCACAAACTCCAAATGCAGATGGACATAGACAGCGGATAATAAATTGGATTGATAGTACAGGACAACTTTCATCTGCTTTTGATTTTACTACCAAGGGAATTCTTCAGTTATTGCTGTTGTATGTGAGGCCATTTCTAACCACCCCCCTCCCTTCTCCAAATAGTAATACCAAGAATACCAGATCTTAATTATTTAAAATAGAAGGGTCAGGCTACAATAATGTCATGGATGTTTCATTTCCCTTTAGACCTGTAACCCTGCAATTTGCGGCTTTTTGTTCATCTTCCACGAACTTCTTTGCTTCTTCTTTCGTATTAAGTGAAGTTTGGTTTTTTTAATATTTCTTCTCGCTAGTACAAGTTAATAATTTACATGCATTTGTTTCTTTGTTTACTACTTAATAAACCTAACTTAATTTGGCATGTAGGAGGCTGTTAAAGGACAATTATGGCGTCTACGTTACCGTCAAGGAAAGCCACCAGGTGTGATGGGATGGTGGCCTTCAAGGGCCGTAACATTCTTAGATAACCATGATACTGGTTCAACACAGGTACCGTCTTTGATAATTATTTATAAAATTATTGCTTGTAGCTGTCAGGCAATTGATAGACACTTCTACATTTGTGTTTTATTGCTCATTATGCGTGCTAATTGGTCTCAATTTGACCATATTTCTTGCTAGCCATTATCCTTTTATTTAAATTAATATTTTAAGACACGTGTTTGGAAGTCATGCCTGTAGTGCGGGCTGAGTGCGACCACAACCATACATGAAAATGTCTTAGACAGCAATGGAGAAGAGCGTTATTGATGCATATATGACCGAAATTATAATGACCGGTTGTCCTACGGCTTTTATATGGCCTATTATGCGTCCAACAGAACAGCTGCTGGGTTCTGTTCCATTTGCTAGTGTTGCTTTCATATTATTACGAGAGGGTGTCATCTTTCATAAGACGTGTATTCATTTTTGCAACAATATATAGCTGAGATGTATGAGGTCAAACTCGAAGTATATTTTAAGTTTTTCTATATTTGTTCCAGATGCCTGTTTGAGTTTTGAACTAATTATATATAAATAGTCCCAGCAATCCAACTCAGCACCTTAATGAGTAGATAGCTTTGGATTATTATTTGGAAACTCATAATTGACCCTGTTCTAGTTTGGTGGTTCTATAATGTATTATATTTAGTTACATTTTTTTGTGACGTTTTGAATCTTACTAGTGAAACATCAACTATTTCTCGTCCCCCCTGCTATGAGAAGAGTTAAGGTTGAAAGCAGCATCAAACAGTGCTTTCACTTAGAGCAATGGGCTAAGCAGATCATGATTAATAATTAATAAATGCAAGTTTACTTGCTTGACGACTAAATCTATATAATTCTTCATGTGTATTTTCTTGAAATATATTTAGAAGCTGGACTTTTACCCCTGGAATGTCAGTAATTCCTCTAACCTCATCATGCTATTATTCTTGCAGGCCCATTGGCCTTTTCCTGCCAACCATGTCATGGATGTACGCAAACTGCGTCTTCATTGCATATGCAATCTCCTTTCTTGCTTGCAGTCTAATTTTTAGCTGTAGCTCTCTTTCATGCTATGCCTGTCCTGGTTTTATAGTACAGTCTAAATACCCAGTCACATGTTCATCATCATTCATGGTGCATTTTCGAAACTCTGAATTGTTTGGTCCAACTAATCCAATGTCTGAATCCAGCCCCATCTTCCCGCATTAGACAATTTAAGTAAGTTATTCTCAGATATTTGACTTCATTTTAAGTCGGTTTCCAACTTTTAACTGCAAATTTTTGGAGTAAACAGTTCAGTGTGCCGAGTGATCAAGCTCTGATGGTATACAAAAGTTTTCTGGGTTAACTGCGTTAGAAAAGCTTTATATTTATCTTCTCTTCTTACAGTTGCTACTGCAACTACTTTTATGCTCGATCTATAGCCTTGTCCTATCTTCCAATAATAAAACATCGTCATGCATCTAATTTTGCTTTCTCGAGCTTTGGTTTAGTAGTCATCCATGATTCCATGCAGGGCTATGCATATATTCTCACGCACCCAGGGATACCAACGGTATTTTATGACCACTTCTACGATTGGGGTGACTCGGTGCACAGGCAGATTGTAAATTTGGTATGTTGCATGCTATTTATTTTATTTTGTTTGCTATCCTTTCTTCAACCAAATTATTCACGTTAATTATTTATATTTTTCTTGCAGATTGACATTCGAAAATGTCAAGACATCCACAGTCGGTCATCTGTGAAGATCCTTGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGGGAAACTGTGCATGAAGATCGGAGATGGTTCTTGGTGCCCTTCCGGTACGGAATGGACGCTTGCAACCTCTGGCCGCAGATATGCAGTATGGCAGAAATAA

mRNA sequence

ATGGTTACCATTGCATTTCCTGAAACCGACAGTTGCTTAAGGCTTAAGCCACTGCCGAAAAATGGACATCCCTATTTTCTTCAACTAAGGCGTCAAGGGGCAGCGATACGCAATGGAAGAGAAATCCTTTTCCAGAAAATGAAGCAGTGCAAGGTTAGAGCAACTGCTGATATAGTCATCAATCACCATGGAATACCATTACCATGGAATGAATTTGCTGTTACCTCATGCACTGGCGGCCTGGGCAATCGTAGCACTGGGGACAACTTCCATGGGGTTCCAAACATCGATCATAGCCAACGTTTTGTCCGTAGAGATATCATAGAATGGCTGAAGTGGCTACGCGGTAATGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGAGGTTATTCTGCAAAATTTGTGAAAGAATATATTGAAGCAGCAAAGCCGATATTTTCTATTGGAGAGTATTGGGATTCTTGTAATTATAATGGCAATGACTTGGACCATAATCAAGATGGACATAGACAGCGGATAATAAATTGGATTGATAGTACAGGACAACTTTCATCTGCTTTTGATTTTACTACCAAGGGAATTCTTCAGTTATTGCTGTTGTATGAGGCTGTTAAAGGACAATTATGGCGTCTACGTTACCGTCAAGGAAAGCCACCAGGTGTGATGGGATGGTGGCCTTCAAGGGCCGTAACATTCTTAGATAACCATGATACTGGTTCAACACAGGCCCATTGGCCTTTTCCTGCCAACCATGTCATGGATGGCTATGCATATATTCTCACGCACCCAGGGATACCAACGGTATTTTATGACCACTTCTACGATTGGGGTGACTCGGTGCACAGGCAGATTGTAAATTTGATTGACATTCGAAAATGTCAAGACATCCACAGTCGGTCATCTGTGAAGATCCTTGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGGGAAACTGTGCATGAAGATCGGAGATGGTTCTTGGTGCCCTTCCGGTACGGAATGGACGCTTGCAACCTCTGGCCGCAGATATGCAGTATGGCAGAAATAA

Coding sequence (CDS)

ATGGTTACCATTGCATTTCCTGAAACCGACAGTTGCTTAAGGCTTAAGCCACTGCCGAAAAATGGACATCCCTATTTTCTTCAACTAAGGCGTCAAGGGGCAGCGATACGCAATGGAAGAGAAATCCTTTTCCAGAAAATGAAGCAGTGCAAGGTTAGAGCAACTGCTGATATAGTCATCAATCACCATGGAATACCATTACCATGGAATGAATTTGCTGTTACCTCATGCACTGGCGGCCTGGGCAATCGTAGCACTGGGGACAACTTCCATGGGGTTCCAAACATCGATCATAGCCAACGTTTTGTCCGTAGAGATATCATAGAATGGCTGAAGTGGCTACGCGGTAATGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGAGGTTATTCTGCAAAATTTGTGAAAGAATATATTGAAGCAGCAAAGCCGATATTTTCTATTGGAGAGTATTGGGATTCTTGTAATTATAATGGCAATGACTTGGACCATAATCAAGATGGACATAGACAGCGGATAATAAATTGGATTGATAGTACAGGACAACTTTCATCTGCTTTTGATTTTACTACCAAGGGAATTCTTCAGTTATTGCTGTTGTATGAGGCTGTTAAAGGACAATTATGGCGTCTACGTTACCGTCAAGGAAAGCCACCAGGTGTGATGGGATGGTGGCCTTCAAGGGCCGTAACATTCTTAGATAACCATGATACTGGTTCAACACAGGCCCATTGGCCTTTTCCTGCCAACCATGTCATGGATGGCTATGCATATATTCTCACGCACCCAGGGATACCAACGGTATTTTATGACCACTTCTACGATTGGGGTGACTCGGTGCACAGGCAGATTGTAAATTTGATTGACATTCGAAAATGTCAAGACATCCACAGTCGGTCATCTGTGAAGATCCTTGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGGGAAACTGTGCATGAAGATCGGAGATGGTTCTTGGTGCCCTTCCGGTACGGAATGGACGCTTGCAACCTCTGGCCGCAGATATGCAGTATGGCAGAAATAA

Protein sequence

MVTIAFPETDSCLRLKPLPKNGHPYFLQLRRQGAAIRNGREILFQKMKQCKVRATADIVINHHGIPLPWNEFAVTSCTGGLGNRSTGDNFHGVPNIDHSQRFVRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIFSIGEYWDSCNYNGNDLDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKGQLWRLRYRQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIPTVFYDHFYDWGDSVHRQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGDGSWCPSGTEWTLATSGRRYAVWQK

Homology

[Close]

BLAST of Moc11g00830 vs. NCBI nr
Match: XP_022132938.1 (LOW QUALITY PROTEIN: probable alpha-amylase 2 [Momordica charantia])

HSP 1 Score: 667.2 bits (1720), Expect = 8.0e-188
Identity = 307/309 (99.35%), Postives = 307/309 (99.35%), Query Frame = 0


Query: 43  LFQKMKQCKVRATADIVINHHGIPLPWNEFAVTSCTGGLGNRSTGDNFHGVPNIDHSQRF 102
           L  KMKQCKVRATADIVINHHGIPLPWNEFAVTSCTGGLGNRSTGDNFHGVPNIDHSQRF
Sbjct: 95  LLXKMKQCKVRATADIVINHHGIPLPWNEFAVTSCTGGLGNRSTGDNFHGVPNIDHSQRF 154

Query: 103 VRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIFSIGEYWDSCNYNGND 162
           VRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIFSIGEYWDSCNYNGND
Sbjct: 155 VRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIFSIGEYWDSCNYNGND 214

Query: 163 LDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKGQLWRLRYRQGKPPGV 222
           LDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKGQLWRLRYRQGKPPGV
Sbjct: 215 LDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKGQLWRLRYRQGKPPGV 274

Query: 223 MGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIPTVFYDHFYDWGDSVH 282
           MGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIPTVFYDHFYDWGDSVH
Sbjct: 275 MGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIPTVFYDHFYDWGDSVH 334

Query: 283 RQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGDGSWCPSGTEWTLATS 342
           RQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGDGSWCPSGTEWTLATS
Sbjct: 335 RQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGDGSWCPSGTEWTLATS 394

Query: 343 GRRYAVWQK 352
           GRRYAVWQK
Sbjct: 395 GRRYAVWQK 403

[Close]

BLAST of Moc11g00830 vs. NCBI nr
Match: XP_011654252.1 (probable alpha-amylase 2 [Cucumis sativus] >XP_031739646.1 probable alpha-amylase 2 [Cucumis sativus])

HSP 1 Score: 569.3 bits (1466), Expect = 2.3e-158
Identity = 268/324 (82.72%), Postives = 284/324 (87.65%), Query Frame = 0


Query: 43  LFQKMKQCKVRATADIVINHH---------------GIPLPWNEFAVTSCTGGLGNRSTG 102
           L  KMKQCKVRA ADIVINH                GI LPWNE+AVTSC+GGLGNRSTG
Sbjct: 95  LLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTG 154

Query: 103 DNFHGVPNIDHSQRFVRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIF 162
           DNFHGVPNIDH+Q FVRRDII WLKWLR +VGFQDFRFDFARG+SAKFVKEYIEA KP+ 
Sbjct: 155 DNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPML 214

Query: 163 SIGEYWDSCNYNGNDLDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKG 222
           SIGEYWDSCNYNG+DLD+NQDGHRQRIINWID TGQLS+AFDFTTKG+LQ     EAV+ 
Sbjct: 215 SIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFTTKGVLQ-----EAVRR 274

Query: 223 QLWRLRYRQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIP 282
           QLWRLR  QGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH+M+GYAYILTHPGIP
Sbjct: 275 QLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIP 334

Query: 283 TVFYDHFYDWGDSVHRQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGD 342
           TVFYDH Y+WGDSVH +IV LIDIRK QDI+SRSSVKILEARSDLYSAIIG KLCMKIGD
Sbjct: 335 TVFYDHLYEWGDSVHDEIVRLIDIRKHQDINSRSSVKILEARSDLYSAIIGEKLCMKIGD 394

Query: 343 GSWCPSGTEWTLATSGRRYAVWQK 352
           GSWCPSGTEWTLATSG RYAVWQK
Sbjct: 395 GSWCPSGTEWTLATSGWRYAVWQK 413

[Close]

BLAST of Moc11g00830 vs. NCBI nr
Match: KAA0064621.1 (putative alpha-amylase 2 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 562.8 bits (1449), Expect = 2.1e-156
Identity = 268/324 (82.72%), Postives = 283/324 (87.35%), Query Frame = 0


Query: 43  LFQKMKQCKVRATADIVINHH---------------GIPLPWNEFAVTSCTGGLGNRSTG 102
           L  KMKQCKVRA ADIVINH                GI LPW+E AVTSC+GGLGNRSTG
Sbjct: 150 LLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTG 209

Query: 103 DNFHGVPNIDHSQRFVRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIF 162
           DNFHGVPNIDH+Q FVRRDII WLKWLR +VGFQDFRFDFARG+SAKFVKEYIEAAKP+F
Sbjct: 210 DNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMF 269

Query: 163 SIGEYWDSCNYNGNDLDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKG 222
           SIGEYWDSCNYNG+DLD+NQDGHRQRIINWID TGQLS+AFDFTTKG+LQ     EAVKG
Sbjct: 270 SIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFTTKGVLQ-----EAVKG 329

Query: 223 QLWRLRYRQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIP 282
           QLWRL   +GKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH+M+GYAYILTHPGIP
Sbjct: 330 QLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIP 389

Query: 283 TVFYDHFYDWGDSVHRQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGD 342
           TVFYDHFYDWGDSVH +IV LIDIRK QDI+SRSSVKILEARSDLYSAIIG  LCMKIGD
Sbjct: 390 TVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSAIIGENLCMKIGD 449

Query: 343 GSWCPSGTEWTLATSGRRYAVWQK 352
           GSWCP GTEWTLAT G RYAVWQK
Sbjct: 450 GSWCP-GTEWTLATYGCRYAVWQK 467

[Close]

BLAST of Moc11g00830 vs. NCBI nr
Match: XP_008452915.1 (PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 562.8 bits (1449), Expect = 2.1e-156
Identity = 268/324 (82.72%), Postives = 283/324 (87.35%), Query Frame = 0


Query: 43  LFQKMKQCKVRATADIVINHH---------------GIPLPWNEFAVTSCTGGLGNRSTG 102
           L  KMKQCKVRA ADIVINH                GI LPW+E AVTSC+GGLGNRSTG
Sbjct: 120 LLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTG 179

Query: 103 DNFHGVPNIDHSQRFVRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIF 162
           DNFHGVPNIDH+Q FVRRDII WLKWLR +VGFQDFRFDFARG+SAKFVKEYIEAAKP+F
Sbjct: 180 DNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMF 239

Query: 163 SIGEYWDSCNYNGNDLDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKG 222
           SIGEYWDSCNYNG+DLD+NQDGHRQRIINWID TGQLS+AFDFTTKG+LQ     EAVKG
Sbjct: 240 SIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFTTKGVLQ-----EAVKG 299

Query: 223 QLWRLRYRQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIP 282
           QLWRL   +GKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH+M+GYAYILTHPGIP
Sbjct: 300 QLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIP 359

Query: 283 TVFYDHFYDWGDSVHRQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGD 342
           TVFYDHFYDWGDSVH +IV LIDIRK QDI+SRSSVKILEARSDLYSAIIG  LCMKIGD
Sbjct: 360 TVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSAIIGENLCMKIGD 419

Query: 343 GSWCPSGTEWTLATSGRRYAVWQK 352
           GSWCP GTEWTLAT G RYAVWQK
Sbjct: 420 GSWCP-GTEWTLATYGCRYAVWQK 437

[Close]

BLAST of Moc11g00830 vs. NCBI nr
Match: KGN55476.1 (hypothetical protein Csa_012097 [Cucumis sativus])

HSP 1 Score: 560.8 bits (1444), Expect = 8.1e-156
Identity = 266/324 (82.10%), Postives = 282/324 (87.04%), Query Frame = 0


Query: 43  LFQKMKQCKVRATADIVINHH---------------GIPLPWNEFAVTSCTGGLGNRSTG 102
           L  KMKQCKVRA ADIVINH                GI LPWNE+AVTSC+GGLGNRSTG
Sbjct: 95  LLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTG 154

Query: 103 DNFHGVPNIDHSQRFVRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIF 162
           DNFHGVPNIDH+Q FVRRDII WLKWLR +VGFQDFRFDFARG+SAKFVKEYIEA KP+ 
Sbjct: 155 DNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPML 214

Query: 163 SIGEYWDSCNYNGNDLDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKG 222
           SIGEYWDSCNYNG+DLD+NQDGHRQRIINWID TGQLS+AFDFTTKG+LQ     EAV+ 
Sbjct: 215 SIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFTTKGVLQ-----EAVRR 274

Query: 223 QLWRLRYRQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIP 282
           QLWRLR  QGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH+M+GYAYILTHPGIP
Sbjct: 275 QLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIP 334

Query: 283 TVFYDHFYDWGDSVHRQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGD 342
           TVFYDH Y+WGDSVH +I   IDIRK QDI+SRSSVKILEARSDLYSAIIG KLCMKIGD
Sbjct: 335 TVFYDHLYEWGDSVHDEI---IDIRKHQDINSRSSVKILEARSDLYSAIIGEKLCMKIGD 394

Query: 343 GSWCPSGTEWTLATSGRRYAVWQK 352
           GSWCPSGTEWTLATSG RYAVWQK
Sbjct: 395 GSWCPSGTEWTLATSGWRYAVWQK 410

[Close]

BLAST of Moc11g00830 vs. ExPASy Swiss-Prot
Match: Q8LFG1 (Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 4.0e-150
Identity = 244/324 (75.31%), Postives = 275/324 (84.88%), Query Frame = 0


Query: 43  LFQKMKQCKVRATADIVINHH---------------GIPLPWNEFAVTSCTGGLGNRSTG 102
           L +KMKQ KVRA ADIVINH                GI LPW+E AVTSCTGGLGNRSTG
Sbjct: 95  LLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTG 154

Query: 103 DNFHGVPNIDHSQRFVRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIF 162
           DNF+GVPN+DH+Q FVR+DII WL+WLR  VGFQDFRFDFARGYSA +VKEYI AAKP+F
Sbjct: 155 DNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLF 214

Query: 163 SIGEYWDSCNYNGNDLDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKG 222
           S+GE WDSCNYNG+ LD+NQD HRQRII+WID+TGQ+S+AFDFTTKGILQ     EAVKG
Sbjct: 215 SVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTTKGILQ-----EAVKG 274

Query: 223 QLWRLRYRQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIP 282
           Q WRL   QGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFP++HVM+GYAYILTHPGIP
Sbjct: 275 QYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEGYAYILTHPGIP 334

Query: 283 TVFYDHFYDWGDSVHRQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGD 342
            VFYDHFYDWG S+H QIV LIDIR+ QDIHSRS+V++L+A S+LY+AI+G K+CMK+GD
Sbjct: 335 CVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAIVGEKICMKLGD 394

Query: 343 GSWCPSGTEWTLATSGRRYAVWQK 352
           GSWCPSG +WTLATSG RYAVW K
Sbjct: 395 GSWCPSGRDWTLATSGHRYAVWHK 413

[Close]

BLAST of Moc11g00830 vs. ExPASy Swiss-Prot
Match: Q94A41 (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 4.9e-87
Identity = 156/325 (48.00%), Postives = 210/325 (64.62%), Query Frame = 0


Query: 45  QKMKQCKVRATADIVINHH--------------GIPLPWNEFAVTSCT---GGLGNRSTG 104
           +K  +  ++   D V+NH               G  L W++ AV +      G GN+S+G
Sbjct: 568 KKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGGRLNWDDRAVVADDPHFQGRGNKSSG 627

Query: 105 DNFHGVPNIDHSQRFVRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIF 164
           DNFH  PNIDHSQ FVR+DI EWL W+   VG+  +R DF RG+   +VK+Y++A+KP F
Sbjct: 628 DNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYVKDYMDASKPYF 687

Query: 165 SIGEYWDSCNYNGNDLDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKG 224
           ++GEYWDS +Y   ++D+NQD HRQRI++WI++T   + AFD TTKGIL   L     K 
Sbjct: 688 AVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGILHTAL----QKC 747

Query: 225 QLWRLRYRQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIP 284
           + WRL   +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M GYAYILTHPG P
Sbjct: 748 EYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYILTHPGTP 807

Query: 285 TVFYDHFYDWGDSVHRQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGD 344
            VF+DH +      H +I  L+ +R  Q +H RS V I ++  D+Y+AII  K+ MKIG 
Sbjct: 808 AVFFDHIF---SDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAMKIGP 867

Query: 345 GSW-CPSGTE-WTLATSGRRYAVWQ 351
           G +  P+G++ W++A  GR Y VW+
Sbjct: 868 GHYEPPNGSQNWSVAVEGRDYKVWE 885

[Close]

BLAST of Moc11g00830 vs. ExPASy Swiss-Prot
Match: P04063 (Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3)

HSP 1 Score: 299.3 bits (765), Expect = 5.8e-80
Identity = 152/330 (46.06%), Postives = 204/330 (61.82%), Query Frame = 0


Query: 52  VRATADIVINHH----------------GIP---LPWNEFAV----TSCTGGLGNRSTGD 111
           V+A ADIVINH                 G P   L W    +         G GN  TG 
Sbjct: 106 VKAIADIVINHRTAEHKDGRGIYCIFEGGTPDARLDWGPHMICRDDRPYADGTGNPDTGA 165

Query: 112 NFHGVPNIDHSQRFVRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIFS 171
           +F   P+IDH    V+++++EWL WL+ ++GF  +RFDFA+GYSA   K YI+ ++P F+
Sbjct: 166 DFGAAPDIDHLNLRVQKELVEWLNWLKADIGFDGWRFDFAKGYSADVAKIYIDRSEPSFA 225

Query: 172 IGEYWDSCNYNGNDLDH-NQDGHRQRIINWIDSTGQLSSA--FDFTTKGILQLLLLYEAV 231
           + E W S  Y G+   + NQD HRQ ++NW+D  G    A  FDFTTKGIL +     AV
Sbjct: 226 VAEIWTSLAYGGDGKPNLNQDQHRQELVNWVDKVGGKGPATTFDFTTKGILNV-----AV 285

Query: 232 KGQLWRLRYRQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPG 291
           +G+LWRLR   GK PG++GWWP++AVTF+DNHDTGSTQ  WPFP++ VM GYAYILTHPG
Sbjct: 286 EGELWRLRGTDGKAPGMIGWWPAKAVTFVDNHDTGSTQHMWPFPSDRVMQGYAYILTHPG 345

Query: 292 IPTVFYDHFYDWGDSVHRQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKI 351
            P +FYDHF+DWG  +  +I  L+ +R    IH+ S ++I+EA +DLY A I GK+ +K+
Sbjct: 346 TPCIFYDHFFDWG--LKEEIDRLVSVRTRHGIHNESKLQIIEADADLYLAEIDGKVIVKL 405

[Close]

BLAST of Moc11g00830 vs. ExPASy Swiss-Prot
Match: P04750 (Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.6 PE=2 SV=2)

HSP 1 Score: 289.3 bits (739), Expect = 6.0e-77
Identity = 153/332 (46.08%), Postives = 202/332 (60.84%), Query Frame = 0


Query: 52  VRATADIVINHH----------------GIP---LPWNEFAV----TSCTGGLGNRSTGD 111
           V+A ADIVINH                 G P   L W    +         G GNR T  
Sbjct: 106 VKAIADIVINHRTAERKDGRGIYCIFEGGTPDARLDWGPHMICRDDRPYPDGTGNRPTRT 165

Query: 112 --NFHGVPNIDHSQRFVRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPI 171
             +F   P+IDH    V+++++EWL WLR + GF  +RFDFA+GYSA   K Y++ ++P 
Sbjct: 166 RADFGAAPDIDHLNPRVQKELVEWLNWLRTDDGFDGWRFDFAKGYSADVAKIYVDRSEPS 225

Query: 172 FSIGEYWDSCNYNGNDLDH-NQDGHRQRIINWIDSTGQLSSA--FDFTTKGILQLLLLYE 231
           F++ E W S  Y G+   + NQD HRQ ++NW++  G    A  FDFTTKGIL +     
Sbjct: 226 FAVAEIWTSLAYGGDGKPNLNQDPHRQELVNWVNKVGGSGPATTFDFTTKGILNV----- 285

Query: 232 AVKGQLWRLRYRQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTH 291
           AV+G+LWRLR   GK PG++GWWP++AVTF+DNHDTGSTQ  WPFP++ VM GYAYILTH
Sbjct: 286 AVEGELWRLRGTDGKAPGMIGWWPAKAVTFVDNHDTGSTQHMWPFPSDRVMQGYAYILTH 345

Query: 292 PGIPTVFYDHFYDWGDSVHRQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCM 351
           PG P +FYDHF+DWG  +  +I  L+ IR  Q IHS S ++I+EA +DLY A I GK+ +
Sbjct: 346 PGNPCIFYDHFFDWG--LKEEIDRLVSIRTRQGIHSESKLQIMEADADLYLAEIEGKVIV 405

[Close]

BLAST of Moc11g00830 vs. ExPASy Swiss-Prot
Match: P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.3e-76
Identity = 155/358 (43.30%), Postives = 211/358 (58.94%), Query Frame = 0


Query: 22  GHPYFLQLRRQGAAIRNGREILFQKMKQCKVRATADIVINHH----------------GI 81
           G  Y L   + G+  +N  + L     +  ++  ADIVINH                 G 
Sbjct: 76  GRLYDLDASKYGS--KNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGT 135

Query: 82  P---LPWNEFAV----TSCTGGLGNRSTGDNFHGVPNIDHSQRFVRRDIIEWLKWLRGNV 141
           P     W    +    T+ + G GN  +G+ +   P+IDH    V+R++ EW+ WL+  +
Sbjct: 136 PDSRQDWGPSFICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELSEWMNWLKTEI 195

Query: 142 GFQDFRFDFARGYSAKFVKEYIEAAKPIFSIGEYWDSCNYNGND--LDHNQDGHRQRIIN 201
           GF  +RFDF +GY+    K Y+E  KP F++GE WDS +Y G D   ++NQD HR  ++N
Sbjct: 196 GFDGWRFDFVKGYAPSISKIYMEQTKPDFAVGEKWDSISY-GQDGKPNYNQDSHRGALVN 255

Query: 202 WIDSTGQLSSAFDFTTKGILQLLLLYEAVKGQLWRLRYRQGKPPGVMGWWPSRAVTFLDN 261
           W++S G   +AFDFTTKGILQ      AV+G+LWRL    GKPPG++G  P  AVTF+DN
Sbjct: 256 WVESAGGAITAFDFTTKGILQ-----AAVQGELWRLIDPNGKPPGMIGVKPENAVTFIDN 315

Query: 262 HDTGSTQAHWPFPANHVMDGYAYILTHPGIPTVFYDHFYDWGDSVHRQIVNLIDIRKCQD 321
           HDTGSTQ  WPFP++ VM GYAYILTHPG P++FYDHF+DWG  +  QI  L  IR    
Sbjct: 316 HDTGSTQRLWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWG--LKEQIAKLSSIRLRNG 375

Query: 322 IHSRSSVKILEARSDLYSAIIGGKLCMKIGD----GSWCPSGTEWTLATSGRRYAVWQ 351
           I+ +S+VKI+ +  DLY A I  K+ +KIG     G+  PS     +ATSG+ YAVW+
Sbjct: 376 INEKSTVKIMASEGDLYVAKIDNKIMVKIGPKMDLGNLIPSNLH--VATSGQDYAVWE 421

[Close]

BLAST of Moc11g00830 vs. ExPASy TrEMBL
Match: A0A6J1BXN7 (1,4-alpha-D-glucan glucanohydrolase OS=Momordica charantia OX=3673 GN=LOC111005656 PE=4 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 3.9e-188
Identity = 307/309 (99.35%), Postives = 307/309 (99.35%), Query Frame = 0


Query: 43  LFQKMKQCKVRATADIVINHHGIPLPWNEFAVTSCTGGLGNRSTGDNFHGVPNIDHSQRF 102
           L  KMKQCKVRATADIVINHHGIPLPWNEFAVTSCTGGLGNRSTGDNFHGVPNIDHSQRF
Sbjct: 95  LLXKMKQCKVRATADIVINHHGIPLPWNEFAVTSCTGGLGNRSTGDNFHGVPNIDHSQRF 154

Query: 103 VRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIFSIGEYWDSCNYNGND 162
           VRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIFSIGEYWDSCNYNGND
Sbjct: 155 VRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIFSIGEYWDSCNYNGND 214

Query: 163 LDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKGQLWRLRYRQGKPPGV 222
           LDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKGQLWRLRYRQGKPPGV
Sbjct: 215 LDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKGQLWRLRYRQGKPPGV 274

Query: 223 MGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIPTVFYDHFYDWGDSVH 282
           MGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIPTVFYDHFYDWGDSVH
Sbjct: 275 MGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIPTVFYDHFYDWGDSVH 334

Query: 283 RQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGDGSWCPSGTEWTLATS 342
           RQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGDGSWCPSGTEWTLATS
Sbjct: 335 RQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGDGSWCPSGTEWTLATS 394

Query: 343 GRRYAVWQK 352
           GRRYAVWQK
Sbjct: 395 GRRYAVWQK 403

[Close]

BLAST of Moc11g00830 vs. ExPASy TrEMBL
Match: A0A5A7VG02 (Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003440 PE=3 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 1.0e-156
Identity = 268/324 (82.72%), Postives = 283/324 (87.35%), Query Frame = 0


Query: 43  LFQKMKQCKVRATADIVINHH---------------GIPLPWNEFAVTSCTGGLGNRSTG 102
           L  KMKQCKVRA ADIVINH                GI LPW+E AVTSC+GGLGNRSTG
Sbjct: 150 LLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTG 209

Query: 103 DNFHGVPNIDHSQRFVRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIF 162
           DNFHGVPNIDH+Q FVRRDII WLKWLR +VGFQDFRFDFARG+SAKFVKEYIEAAKP+F
Sbjct: 210 DNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMF 269

Query: 163 SIGEYWDSCNYNGNDLDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKG 222
           SIGEYWDSCNYNG+DLD+NQDGHRQRIINWID TGQLS+AFDFTTKG+LQ     EAVKG
Sbjct: 270 SIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFTTKGVLQ-----EAVKG 329

Query: 223 QLWRLRYRQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIP 282
           QLWRL   +GKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH+M+GYAYILTHPGIP
Sbjct: 330 QLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIP 389

Query: 283 TVFYDHFYDWGDSVHRQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGD 342
           TVFYDHFYDWGDSVH +IV LIDIRK QDI+SRSSVKILEARSDLYSAIIG  LCMKIGD
Sbjct: 390 TVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSAIIGENLCMKIGD 449

Query: 343 GSWCPSGTEWTLATSGRRYAVWQK 352
           GSWCP GTEWTLAT G RYAVWQK
Sbjct: 450 GSWCP-GTEWTLATYGCRYAVWQK 467

[Close]

BLAST of Moc11g00830 vs. ExPASy TrEMBL
Match: A0A1S3BW59 (Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 1.0e-156
Identity = 268/324 (82.72%), Postives = 283/324 (87.35%), Query Frame = 0


Query: 43  LFQKMKQCKVRATADIVINHH---------------GIPLPWNEFAVTSCTGGLGNRSTG 102
           L  KMKQCKVRA ADIVINH                GI LPW+E AVTSC+GGLGNRSTG
Sbjct: 120 LLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTG 179

Query: 103 DNFHGVPNIDHSQRFVRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIF 162
           DNFHGVPNIDH+Q FVRRDII WLKWLR +VGFQDFRFDFARG+SAKFVKEYIEAAKP+F
Sbjct: 180 DNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMF 239

Query: 163 SIGEYWDSCNYNGNDLDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKG 222
           SIGEYWDSCNYNG+DLD+NQDGHRQRIINWID TGQLS+AFDFTTKG+LQ     EAVKG
Sbjct: 240 SIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFTTKGVLQ-----EAVKG 299

Query: 223 QLWRLRYRQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIP 282
           QLWRL   +GKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH+M+GYAYILTHPGIP
Sbjct: 300 QLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIP 359

Query: 283 TVFYDHFYDWGDSVHRQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGD 342
           TVFYDHFYDWGDSVH +IV LIDIRK QDI+SRSSVKILEARSDLYSAIIG  LCMKIGD
Sbjct: 360 TVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSAIIGENLCMKIGD 419

Query: 343 GSWCPSGTEWTLATSGRRYAVWQK 352
           GSWCP GTEWTLAT G RYAVWQK
Sbjct: 420 GSWCP-GTEWTLATYGCRYAVWQK 437

[Close]

BLAST of Moc11g00830 vs. ExPASy TrEMBL
Match: A0A0A0L5R6 (Alpha-amylase OS=Cucumis sativus OX=3659 GN=Csa_4G652880 PE=3 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 3.9e-156
Identity = 266/324 (82.10%), Postives = 282/324 (87.04%), Query Frame = 0


Query: 43  LFQKMKQCKVRATADIVINHH---------------GIPLPWNEFAVTSCTGGLGNRSTG 102
           L  KMKQCKVRA ADIVINH                GI LPWNE+AVTSC+GGLGNRSTG
Sbjct: 95  LLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTG 154

Query: 103 DNFHGVPNIDHSQRFVRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIF 162
           DNFHGVPNIDH+Q FVRRDII WLKWLR +VGFQDFRFDFARG+SAKFVKEYIEA KP+ 
Sbjct: 155 DNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPML 214

Query: 163 SIGEYWDSCNYNGNDLDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKG 222
           SIGEYWDSCNYNG+DLD+NQDGHRQRIINWID TGQLS+AFDFTTKG+LQ     EAV+ 
Sbjct: 215 SIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFTTKGVLQ-----EAVRR 274

Query: 223 QLWRLRYRQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIP 282
           QLWRLR  QGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH+M+GYAYILTHPGIP
Sbjct: 275 QLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIP 334

Query: 283 TVFYDHFYDWGDSVHRQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGD 342
           TVFYDH Y+WGDSVH +I   IDIRK QDI+SRSSVKILEARSDLYSAIIG KLCMKIGD
Sbjct: 335 TVFYDHLYEWGDSVHDEI---IDIRKHQDINSRSSVKILEARSDLYSAIIGEKLCMKIGD 394

Query: 343 GSWCPSGTEWTLATSGRRYAVWQK 352
           GSWCPSGTEWTLATSG RYAVWQK
Sbjct: 395 GSWCPSGTEWTLATSGWRYAVWQK 410

[Close]

BLAST of Moc11g00830 vs. ExPASy TrEMBL
Match: A0A5D3D8R2 (Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00850 PE=3 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 5.7e-155
Identity = 268/328 (81.71%), Postives = 283/328 (86.28%), Query Frame = 0


Query: 43  LFQKMKQCKVRATADIVINHH---------------GIPLPWNEFAVTSCTGGLGNRSTG 102
           L  KMKQCKVRA ADIVINH                GI LPW+E AVTSC+GGLGNRSTG
Sbjct: 150 LLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTG 209

Query: 103 DNFHGVPNIDHSQRFVRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIF 162
           DNFHGVPNIDH+Q FVRRDII WLKWLR +VGFQDFRFDFARG+SAKFVKEYIEAAKP+F
Sbjct: 210 DNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMF 269

Query: 163 SIGEYWDSCNYNGNDLDHNQ----DGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYE 222
           SIGEYWDSCNYNG+DLD+NQ    DGHRQRIINWID TGQLS+AFDFTTKG+LQ     E
Sbjct: 270 SIGEYWDSCNYNGHDLDYNQALNADGHRQRIINWIDGTGQLSAAFDFTTKGVLQ-----E 329

Query: 223 AVKGQLWRLRYRQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTH 282
           AVKGQLWRL   +GKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH+M+GYAYILTH
Sbjct: 330 AVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYILTH 389

Query: 283 PGIPTVFYDHFYDWGDSVHRQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCM 342
           PGIPTVFYDHFYDWGDSVH +IV LIDIRK QDI+SRSSVKILEARSDLYSAIIG  LCM
Sbjct: 390 PGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSAIIGENLCM 449

Query: 343 KIGDGSWCPSGTEWTLATSGRRYAVWQK 352
           KIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 450 KIGDGSWCP-GTEWTLATYGCRYAVWQK 471

[Close]

BLAST of Moc11g00830 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 532.3 bits (1370), Expect = 2.9e-151
Identity = 244/324 (75.31%), Postives = 275/324 (84.88%), Query Frame = 0


Query: 43  LFQKMKQCKVRATADIVINHH---------------GIPLPWNEFAVTSCTGGLGNRSTG 102
           L +KMKQ KVRA ADIVINH                GI LPW+E AVTSCTGGLGNRSTG
Sbjct: 95  LLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTG 154

Query: 103 DNFHGVPNIDHSQRFVRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIF 162
           DNF+GVPN+DH+Q FVR+DII WL+WLR  VGFQDFRFDFARGYSA +VKEYI AAKP+F
Sbjct: 155 DNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLF 214

Query: 163 SIGEYWDSCNYNGNDLDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKG 222
           S+GE WDSCNYNG+ LD+NQD HRQRII+WID+TGQ+S+AFDFTTKGILQ     EAVKG
Sbjct: 215 SVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTTKGILQ-----EAVKG 274

Query: 223 QLWRLRYRQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIP 282
           Q WRL   QGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFP++HVM+GYAYILTHPGIP
Sbjct: 275 QYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEGYAYILTHPGIP 334

Query: 283 TVFYDHFYDWGDSVHRQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGD 342
            VFYDHFYDWG S+H QIV LIDIR+ QDIHSRS+V++L+A S+LY+AI+G K+CMK+GD
Sbjct: 335 CVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAIVGEKICMKLGD 394

Query: 343 GSWCPSGTEWTLATSGRRYAVWQK 352
           GSWCPSG +WTLATSG RYAVW K
Sbjct: 395 GSWCPSGRDWTLATSGHRYAVWHK 413

[Close]

BLAST of Moc11g00830 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 322.8 bits (826), Expect = 3.5e-88
Identity = 156/325 (48.00%), Postives = 210/325 (64.62%), Query Frame = 0


Query: 45  QKMKQCKVRATADIVINHH--------------GIPLPWNEFAVTSCT---GGLGNRSTG 104
           +K  +  ++   D V+NH               G  L W++ AV +      G GN+S+G
Sbjct: 568 KKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGGRLNWDDRAVVADDPHFQGRGNKSSG 627

Query: 105 DNFHGVPNIDHSQRFVRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKEYIEAAKPIF 164
           DNFH  PNIDHSQ FVR+DI EWL W+   VG+  +R DF RG+   +VK+Y++A+KP F
Sbjct: 628 DNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYVKDYMDASKPYF 687

Query: 165 SIGEYWDSCNYNGNDLDHNQDGHRQRIINWIDSTGQLSSAFDFTTKGILQLLLLYEAVKG 224
           ++GEYWDS +Y   ++D+NQD HRQRI++WI++T   + AFD TTKGIL   L     K 
Sbjct: 688 AVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGILHTAL----QKC 747

Query: 225 QLWRLRYRQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDGYAYILTHPGIP 284
           + WRL   +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M GYAYILTHPG P
Sbjct: 748 EYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYILTHPGTP 807

Query: 285 TVFYDHFYDWGDSVHRQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAIIGGKLCMKIGD 344
            VF+DH +      H +I  L+ +R  Q +H RS V I ++  D+Y+AII  K+ MKIG 
Sbjct: 808 AVFFDHIF---SDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAMKIGP 867

Query: 345 GSW-CPSGTE-WTLATSGRRYAVWQ 351
           G +  P+G++ W++A  GR Y VW+
Sbjct: 868 GHYEPPNGSQNWSVAVEGRDYKVWE 885

[Close]

BLAST of Moc11g00830 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 258.8 bits (660), Expect = 6.1e-69
Identity = 144/339 (42.48%), Postives = 195/339 (57.52%), Query Frame = 0


Query: 43  LFQKMKQCKVRATADIVINHHGIP-------------------LPWNEFAVTSCTG---- 102
           L + + Q  ++A ADIVINH                       L W+   V  C      
Sbjct: 98  LIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPSFV--CRNDPKF 157

Query: 103 -GLGNRSTGDNFHGVPNIDHSQRFVRRDIIEWLKWLRGNVGFQDFRFDFARGYSAKFVKE 162
            G GN  TG +F G P+IDH    V++++ EW+ WL+  +GF  +RFD+ RGY++   K 
Sbjct: 158 PGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDYVRGYASSITKL 217

Query: 163 YIEAAKPIFSIGEYWDSCNYNGN-DLDHNQDGHRQRIINWIDST-GQLSSAFDFTTKGIL 222
           Y++   P F++GE WD   Y G+  LD++Q+ HR  +  WI+   G + +AFDFTTKGIL
Sbjct: 218 YVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVLTAFDFTTKGIL 277

Query: 223 QLLLLYEAVKGQLWRLRYRQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHVMDG 282
           Q      AVKG+LWRL+  QGKPPG++G  P  AVTF+DNHDT  T   W FP++ V+ G
Sbjct: 278 Q-----SAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRT---WVFPSDKVLLG 337

Query: 283 YAYILTHPGIPTVFYDHFYDWGDSVHRQIVNLIDIRKCQDIHSRSSVKILEARSDLYSAI 342
           Y YILTHPG P +FY+H+ +WG  +   I  L+ IR    I S SSV I  A +DLY A+
Sbjct: 338 YVYILTHPGTPCIFYNHYIEWG--LKESISKLVAIRNKNGIGSTSSVTIKAAEADLYLAM 397

Query: 343 IGGKLCMKIGD----GSWCPSGTEWTLATSGRRYAVWQK 352
           I  K+ MKIG     G+  PS   + LA SG  +AVW+K
Sbjct: 398 IDDKVIMKIGPKQDVGTLVPS--NFALAYSGLDFAVWEK 422

The following BLAST results are available for this feature:

BLAST of Moc11g00830 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132938.1	8.0e-188	99.35	LOW QUALITY PROTEIN: probable alpha-amylase 2 [Momordica charantia]	[more]
XP_011654252.1	2.3e-158	82.72	probable alpha-amylase 2 [Cucumis sativus] >XP_031739646.1 probable alpha-amylas...	[more]
KAA0064621.1	2.1e-156	82.72	putative alpha-amylase 2 isoform X2 [Cucumis melo var. makuwa]	[more]
XP_008452915.1	2.1e-156	82.72	PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo]	[more]
KGN55476.1	8.1e-156	82.10	hypothetical protein Csa_012097 [Cucumis sativus]	[more]

BLAST of Moc11g00830 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LFG1	4.0e-150	75.31	Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1	[more]
Q94A41	4.9e-87	48.00	Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1	[more]
P04063	5.8e-80	46.06	Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3	[more]
P04750	6.0e-77	46.08	Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.6 PE=2 SV=2	[more]
P17859	1.3e-76	43.30	Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1	[more]

BLAST of Moc11g00830 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BXN7	3.9e-188	99.35	1,4-alpha-D-glucan glucanohydrolase OS=Momordica charantia OX=3673 GN=LOC1110056...	[more]
A0A5A7VG02	1.0e-156	82.72	Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003440...	[more]
A0A1S3BW59	1.0e-156	82.72	Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1	[more]
A0A0A0L5R6	3.9e-156	82.10	Alpha-amylase OS=Cucumis sativus OX=3659 GN=Csa_4G652880 PE=3 SV=1	[more]
A0A5D3D8R2	5.7e-155	81.71	Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00850 ...	[more]

BLAST of Moc11g00830 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G76130.1	2.9e-151	75.31	alpha-amylase-like 2	[more]
AT1G69830.1	3.5e-88	48.00	alpha-amylase-like 3	[more]
AT4G25000.1	6.1e-69	42.48	alpha-amylase-like	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	SMART	SM00642	aamy	coord: 1..304 e-value: 1.9E-5 score: -41.1
IPR012850	Alpha-amylase, C-terminal beta-sheet	SMART	SM00810	alpha-amyl_c2	coord: 293..351 e-value: 2.4E-29 score: 113.5
IPR012850	Alpha-amylase, C-terminal beta-sheet	PFAM	PF07821	Alpha-amyl_C2	coord: 295..350 e-value: 1.6E-22 score: 79.5
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 41..296 e-value: 1.2E-81 score: 276.8
None	No IPR available	PANTHER	PTHR43447:SF18	ALPHA-AMYLASE 2-RELATED	coord: 41..351
None	No IPR available	PANTHER	PTHR43447	ALPHA-AMYLASE	coord: 41..351
None	No IPR available	CDD	cd11314	AmyAc_arch_bac_plant_AmyA	coord: 43..303 e-value: 7.17602E-124 score: 356.531
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 297..351
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	2.60.40.1180		coord: 297..351 e-value: 9.8E-20 score: 72.4
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 46..283

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc11g00830.1	Moc11g00830.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004556	alpha-amylase activity
molecular_function	GO:0103025	alpha-amylase activity (releasing maltohexaose)
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0003824	catalytic activity