Moc10g27930 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc10g27930
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRetrovirus-related Pol polyprotein from transposon RE1
Locationchr10: 20804052 .. 20804570 (-)
RNA-Seq ExpressionMoc10g27930
SyntenyMoc10g27930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAAAACAGAAAATAGTCTTACTATTCAATTATTTCATCAATGCTCGAGCCTCATTTCTATTAAGCTCAATTCCAACAACTATCTTCTATGGAAATCTCAGATCCTGCCCCTCATTAGAACTATGGGAATGGAGCAACATCTTACTGATGATCAGCCACCAGAAGAACATGTTGGAAGCACATGTTGGAAGCAGCAAGGAAGACAATATACCATGTGGATAAACAATGATGGTCTCCGGACTTCGTGGCTTTTAGGAACTATCAATGAAGATGTGTTGAGCATGATTGAAAACACAGAAACTGCCTATCAAGTGTGGAGATCATTAGAGGAGCAACTTCTCACAATGACCAAAGAAAACGAGATTCATCTCAACGAGGCGTTACTCAACTTGAAGAAAGGGAATCTCAACCTTGAAGAATATTTGAAGAAATTCAAGGCCCTCACTGACAAATTATCGGCAATGAAGAAGCCCTTAGATGAAACACAAACAATATTTCACTTGGCAAGGGCTTAG

mRNA sequence

ATGGCCAAAACAGAAAATAGTCTTACTATTCAATTATTTCATCAATGCTCGAGCCTCATTTCTATTAAGCTCAATTCCAACAACTATCTTCTATGGAAATCTCAGATCCTGCCCCTCATTAGAACTATGGGAATGGAGCAACATCTTACTGATGATCAGCCACCAGAAGAACATGTTGGAAGCACATGTTGGAAGCAGCAAGGAAGACAATATACCATGTGGATAAACAATGATGGTCTCCGGACTTCGTGGCTTTTAGGAACTATCAATGAAGATGTGTTGAGCATGATTGAAAACACAGAAACTGCCTATCAAGTGTGGAGATCATTAGAGGAGCAACTTCTCACAATGACCAAAGAAAACGAGATTCATCTCAACGAGGCGTTACTCAACTTGAAGAAAGGGAATCTCAACCTTGAAGAATATTTGAAGAAATTCAAGGCCCTCACTGACAAATTATCGGCAATGAAGAAGCCCTTAGATGAAACACAAACAATATTTCACTTGGCAAGGGCTTAG

Coding sequence (CDS)

ATGGCCAAAACAGAAAATAGTCTTACTATTCAATTATTTCATCAATGCTCGAGCCTCATTTCTATTAAGCTCAATTCCAACAACTATCTTCTATGGAAATCTCAGATCCTGCCCCTCATTAGAACTATGGGAATGGAGCAACATCTTACTGATGATCAGCCACCAGAAGAACATGTTGGAAGCACATGTTGGAAGCAGCAAGGAAGACAATATACCATGTGGATAAACAATGATGGTCTCCGGACTTCGTGGCTTTTAGGAACTATCAATGAAGATGTGTTGAGCATGATTGAAAACACAGAAACTGCCTATCAAGTGTGGAGATCATTAGAGGAGCAACTTCTCACAATGACCAAAGAAAACGAGATTCATCTCAACGAGGCGTTACTCAACTTGAAGAAAGGGAATCTCAACCTTGAAGAATATTTGAAGAAATTCAAGGCCCTCACTGACAAATTATCGGCAATGAAGAAGCCCTTAGATGAAACACAAACAATATTTCACTTGGCAAGGGCTTAG

Protein sequence

MAKTENSLTIQLFHQCSSLISIKLNSNNYLLWKSQILPLIRTMGMEQHLTDDQPPEEHVGSTCWKQQGRQYTMWINNDGLRTSWLLGTINEDVLSMIENTETAYQVWRSLEEQLLTMTKENEIHLNEALLNLKKGNLNLEEYLKKFKALTDKLSAMKKPLDETQTIFHLARA
Homology
BLAST of Moc10g27930 vs. NCBI nr
Match: XP_022154021.1 (uncharacterized protein LOC111021379 [Momordica charantia] >XP_022154022.1 uncharacterized protein LOC111021379 [Momordica charantia])

HSP 1 Score: 220.7 bits (561), Expect = 9.7e-54
Identity = 113/172 (65.70%), Postives = 135/172 (78.49%), Query Frame = 0

Query: 1   MAKTENSLTIQLFHQCSSLISIKLNSNNYLLWKSQILPLIRTMGMEQHLTDDQP-PEEHV 60
           MA  EN LT+Q FHQCSSLIS+KLNS+NYLLWKSQ+LPLIRT+G+E HL ++ P  +E  
Sbjct: 1   MALPENLLTVQSFHQCSSLISLKLNSSNYLLWKSQVLPLIRTLGLEHHLXEEAPVVDECK 60

Query: 61  GSTCWKQQGRQYTMWINNDGLRTSWLLGTINEDVLSMIENTETAYQVWRSLEEQLLTMTK 120
           G         Q   WINNDGL TSWLLG I EDVL+++E TETA +VW SLEE LLTMTK
Sbjct: 61  GKEGESAXXTQVRTWINNDGLLTSWLLGIIAEDVLTLLEGTETAKEVWHSLEELLLTMTK 120

Query: 121 ENEIHLNEALLNLKKGNLNLEEYLKKFKALTDKLSAMKKPLDETQTIFHLAR 172
           ENEIHLNEALL LKKG+L+++EY++KFK L D+L AMKKPLD+   +FHLAR
Sbjct: 121 ENEIHLNEALLTLKKGSLSMDEYIRKFKNLCDRLXAMKKPLDDLTKVFHLAR 172

BLAST of Moc10g27930 vs. NCBI nr
Match: RVW19921.1 (Retrovirus-related Pol polyprotein from transposon RE1 [Vitis vinifera])

HSP 1 Score: 172.6 bits (436), Expect = 3.0e-39
Identity = 84/172 (48.84%), Postives = 123/172 (71.51%), Query Frame = 0

Query: 1   MAKTENSLTIQLFHQCSSLISIKLNSNNYLLWKSQILPLIRTMGMEQHLTDDQ-PPEEHV 60
           MA  EN L+IQ FHQCSSL+SIKLN +N LLW+SQ+LPL+R++G+  HL++++   EE +
Sbjct: 15  MANPENVLSIQAFHQCSSLVSIKLNMSNLLLWRSQVLPLVRSLGLIHHLSENRHASEETM 74

Query: 61  GSTCWKQQGRQYTMWINNDGLRTSWLLGTINEDVLSMIENTETAYQVWRSLEEQLLTMTK 120
           G+   +   +    W +NDGL TSWLLG + E+V+ +++ TETAY VW SL E+LL MTK
Sbjct: 75  GTETKETHDQSIETWSHNDGLLTSWLLGLMTEEVMLLLDGTETAYDVWNSLGEKLLPMTK 134

Query: 121 ENEIHLNEALLNLKKGNLNLEEYLKKFKALTDKLSAMKKPLDETQTIFHLAR 172
           E E+ L   L  +KKG  +L+EYL++FK + D L+A++KP+ +   +F LA+
Sbjct: 135 EKEVQLTNRLRGVKKGTRSLDEYLREFKGICDALAAVRKPVSDLDKVFQLAQ 186

BLAST of Moc10g27930 vs. NCBI nr
Match: RVW43526.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 171.0 bits (432), Expect = 8.8e-39
Identity = 83/172 (48.26%), Postives = 123/172 (71.51%), Query Frame = 0

Query: 1   MAKTENSLTIQLFHQCSSLISIKLNSNNYLLWKSQILPLIRTMGMEQHLTDDQ-PPEEHV 60
           MA  EN L+IQ FHQCSSL+SIKLN +N LLW+SQ+LPL+R++G+  HL++++   +E +
Sbjct: 46  MANPENVLSIQAFHQCSSLVSIKLNMSNLLLWRSQVLPLVRSLGLIHHLSENRHASKETM 105

Query: 61  GSTCWKQQGRQYTMWINNDGLRTSWLLGTINEDVLSMIENTETAYQVWRSLEEQLLTMTK 120
           G+   +   +    W +NDGL TSWLLG + E+V+ +++ TETAY VW SL E+LL MTK
Sbjct: 106 GTETKETHDQSIETWSHNDGLLTSWLLGLMTEEVMLLLDGTETAYDVWNSLGEKLLPMTK 165

Query: 121 ENEIHLNEALLNLKKGNLNLEEYLKKFKALTDKLSAMKKPLDETQTIFHLAR 172
           E E+ L   L  +KKG  +L+EYL++FK + D L+A++KP+ +   +F LA+
Sbjct: 166 EKEVQLTNRLRGVKKGTRSLDEYLREFKGICDALAAVRKPVSDLDKVFQLAQ 217

BLAST of Moc10g27930 vs. NCBI nr
Match: RVW93768.1 (Retrovirus-related Pol polyprotein from transposon RE1 [Vitis vinifera])

HSP 1 Score: 171.0 bits (432), Expect = 8.8e-39
Identity = 83/172 (48.26%), Postives = 123/172 (71.51%), Query Frame = 0

Query: 1   MAKTENSLTIQLFHQCSSLISIKLNSNNYLLWKSQILPLIRTMGMEQHLTDDQ-PPEEHV 60
           MA  EN L+IQ FHQCSSL+SIKLN +N LLW+SQ+LPL+R++G+  HL++++   EE +
Sbjct: 1   MANPENVLSIQAFHQCSSLVSIKLNMSNLLLWRSQVLPLVRSLGLIHHLSENRHASEETM 60

Query: 61  GSTCWKQQGRQYTMWINNDGLRTSWLLGTINEDVLSMIENTETAYQVWRSLEEQLLTMTK 120
           G+   +   +    W +NDGL TSWLLG + ++V+ +++ TETAY VW SL E+LL MTK
Sbjct: 61  GTETKETHDQSIETWSHNDGLLTSWLLGLMTKEVMLLLDGTETAYDVWNSLGEKLLPMTK 120

Query: 121 ENEIHLNEALLNLKKGNLNLEEYLKKFKALTDKLSAMKKPLDETQTIFHLAR 172
           E E+ L   L  +KKG  +L+EYL++FK + D L+A++KP+ +   +F LA+
Sbjct: 121 EKEVQLTNRLRGVKKGTRSLDEYLREFKGICDALAAVRKPVSDLDKVFQLAQ 172

BLAST of Moc10g27930 vs. NCBI nr
Match: GAU44375.1 (hypothetical protein TSUD_243070 [Trifolium subterraneum])

HSP 1 Score: 170.6 bits (431), Expect = 1.2e-38
Identity = 87/169 (51.48%), Postives = 120/169 (71.01%), Query Frame = 0

Query: 5   ENSLTIQLFHQCSSLISIKLNSNNYLLWKSQILPLIRTMGMEQHLTDD--QPPEEHVGST 64
           E  LTIQ FHQCSSLISIKL+++N+LLWKSQILPLIR++G+E H+T D  +P +E   S+
Sbjct: 13  EPKLTIQSFHQCSSLISIKLSTSNFLLWKSQILPLIRSLGLEHHITADTSKPDDEITDSS 72

Query: 65  CWKQQGRQYTMWINNDGLRTSWLLGTINEDVLSMIENTETAYQVWRSLEEQLLTMTKENE 124
             K +      WI NDGL TSWLLG + E+ +SMI   +TA+ +W SL EQLL  T++ E
Sbjct: 73  GTKIKNPDAVQWILNDGLLTSWLLGNMKEETVSMILGGDTAHYIWSSLHEQLLPNTEDGE 132

Query: 125 IHLNEALLNLKKGNLNLEEYLKKFKALTDKLSAMKKPLDETQTIFHLAR 172
             L  +L  L KGNL+L+EY++KFK L DKL+A+ KP+ +   +F +++
Sbjct: 133 AQLKNSLYALSKGNLSLDEYIRKFKELCDKLTAIGKPVSDVDKVFQISK 181

BLAST of Moc10g27930 vs. ExPASy TrEMBL
Match: A0A6J1DMG5 (uncharacterized protein LOC111021379 OS=Momordica charantia OX=3673 GN=LOC111021379 PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 4.7e-54
Identity = 113/172 (65.70%), Postives = 135/172 (78.49%), Query Frame = 0

Query: 1   MAKTENSLTIQLFHQCSSLISIKLNSNNYLLWKSQILPLIRTMGMEQHLTDDQP-PEEHV 60
           MA  EN LT+Q FHQCSSLIS+KLNS+NYLLWKSQ+LPLIRT+G+E HL ++ P  +E  
Sbjct: 1   MALPENLLTVQSFHQCSSLISLKLNSSNYLLWKSQVLPLIRTLGLEHHLXEEAPVVDECK 60

Query: 61  GSTCWKQQGRQYTMWINNDGLRTSWLLGTINEDVLSMIENTETAYQVWRSLEEQLLTMTK 120
           G         Q   WINNDGL TSWLLG I EDVL+++E TETA +VW SLEE LLTMTK
Sbjct: 61  GKEGESAXXTQVRTWINNDGLLTSWLLGIIAEDVLTLLEGTETAKEVWHSLEELLLTMTK 120

Query: 121 ENEIHLNEALLNLKKGNLNLEEYLKKFKALTDKLSAMKKPLDETQTIFHLAR 172
           ENEIHLNEALL LKKG+L+++EY++KFK L D+L AMKKPLD+   +FHLAR
Sbjct: 121 ENEIHLNEALLTLKKGSLSMDEYIRKFKNLCDRLXAMKKPLDDLTKVFHLAR 172

BLAST of Moc10g27930 vs. ExPASy TrEMBL
Match: A0A438C9J9 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Vitis vinifera OX=29760 GN=RE1_1040 PE=4 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 1.5e-39
Identity = 84/172 (48.84%), Postives = 123/172 (71.51%), Query Frame = 0

Query: 1   MAKTENSLTIQLFHQCSSLISIKLNSNNYLLWKSQILPLIRTMGMEQHLTDDQ-PPEEHV 60
           MA  EN L+IQ FHQCSSL+SIKLN +N LLW+SQ+LPL+R++G+  HL++++   EE +
Sbjct: 15  MANPENVLSIQAFHQCSSLVSIKLNMSNLLLWRSQVLPLVRSLGLIHHLSENRHASEETM 74

Query: 61  GSTCWKQQGRQYTMWINNDGLRTSWLLGTINEDVLSMIENTETAYQVWRSLEEQLLTMTK 120
           G+   +   +    W +NDGL TSWLLG + E+V+ +++ TETAY VW SL E+LL MTK
Sbjct: 75  GTETKETHDQSIETWSHNDGLLTSWLLGLMTEEVMLLLDGTETAYDVWNSLGEKLLPMTK 134

Query: 121 ENEIHLNEALLNLKKGNLNLEEYLKKFKALTDKLSAMKKPLDETQTIFHLAR 172
           E E+ L   L  +KKG  +L+EYL++FK + D L+A++KP+ +   +F LA+
Sbjct: 135 EKEVQLTNRLRGVKKGTRSLDEYLREFKGICDALAAVRKPVSDLDKVFQLAQ 186

BLAST of Moc10g27930 vs. ExPASy TrEMBL
Match: A0A438IAM2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Vitis vinifera OX=29760 GN=RE1_3180 PE=4 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 4.3e-39
Identity = 83/172 (48.26%), Postives = 123/172 (71.51%), Query Frame = 0

Query: 1   MAKTENSLTIQLFHQCSSLISIKLNSNNYLLWKSQILPLIRTMGMEQHLTDDQ-PPEEHV 60
           MA  EN L+IQ FHQCSSL+SIKLN +N LLW+SQ+LPL+R++G+  HL++++   EE +
Sbjct: 1   MANPENVLSIQAFHQCSSLVSIKLNMSNLLLWRSQVLPLVRSLGLIHHLSENRHASEETM 60

Query: 61  GSTCWKQQGRQYTMWINNDGLRTSWLLGTINEDVLSMIENTETAYQVWRSLEEQLLTMTK 120
           G+   +   +    W +NDGL TSWLLG + ++V+ +++ TETAY VW SL E+LL MTK
Sbjct: 61  GTETKETHDQSIETWSHNDGLLTSWLLGLMTKEVMLLLDGTETAYDVWNSLGEKLLPMTK 120

Query: 121 ENEIHLNEALLNLKKGNLNLEEYLKKFKALTDKLSAMKKPLDETQTIFHLAR 172
           E E+ L   L  +KKG  +L+EYL++FK + D L+A++KP+ +   +F LA+
Sbjct: 121 EKEVQLTNRLRGVKKGTRSLDEYLREFKGICDALAAVRKPVSDLDKVFQLAQ 172

BLAST of Moc10g27930 vs. ExPASy TrEMBL
Match: A0A438E6Z5 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_1575 PE=4 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 4.3e-39
Identity = 83/172 (48.26%), Postives = 123/172 (71.51%), Query Frame = 0

Query: 1   MAKTENSLTIQLFHQCSSLISIKLNSNNYLLWKSQILPLIRTMGMEQHLTDDQ-PPEEHV 60
           MA  EN L+IQ FHQCSSL+SIKLN +N LLW+SQ+LPL+R++G+  HL++++   +E +
Sbjct: 46  MANPENVLSIQAFHQCSSLVSIKLNMSNLLLWRSQVLPLVRSLGLIHHLSENRHASKETM 105

Query: 61  GSTCWKQQGRQYTMWINNDGLRTSWLLGTINEDVLSMIENTETAYQVWRSLEEQLLTMTK 120
           G+   +   +    W +NDGL TSWLLG + E+V+ +++ TETAY VW SL E+LL MTK
Sbjct: 106 GTETKETHDQSIETWSHNDGLLTSWLLGLMTEEVMLLLDGTETAYDVWNSLGEKLLPMTK 165

Query: 121 ENEIHLNEALLNLKKGNLNLEEYLKKFKALTDKLSAMKKPLDETQTIFHLAR 172
           E E+ L   L  +KKG  +L+EYL++FK + D L+A++KP+ +   +F LA+
Sbjct: 166 EKEVQLTNRLRGVKKGTRSLDEYLREFKGICDALAAVRKPVSDLDKVFQLAQ 217

BLAST of Moc10g27930 vs. ExPASy TrEMBL
Match: A0A2Z6P7T0 (Reverse transcriptase Ty1/copia-type domain-containing protein OS=Trifolium subterraneum OX=3900 GN=TSUD_243070 PE=4 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 5.6e-39
Identity = 87/169 (51.48%), Postives = 120/169 (71.01%), Query Frame = 0

Query: 5   ENSLTIQLFHQCSSLISIKLNSNNYLLWKSQILPLIRTMGMEQHLTDD--QPPEEHVGST 64
           E  LTIQ FHQCSSLISIKL+++N+LLWKSQILPLIR++G+E H+T D  +P +E   S+
Sbjct: 13  EPKLTIQSFHQCSSLISIKLSTSNFLLWKSQILPLIRSLGLEHHITADTSKPDDEITDSS 72

Query: 65  CWKQQGRQYTMWINNDGLRTSWLLGTINEDVLSMIENTETAYQVWRSLEEQLLTMTKENE 124
             K +      WI NDGL TSWLLG + E+ +SMI   +TA+ +W SL EQLL  T++ E
Sbjct: 73  GTKIKNPDAVQWILNDGLLTSWLLGNMKEETVSMILGGDTAHYIWSSLHEQLLPNTEDGE 132

Query: 125 IHLNEALLNLKKGNLNLEEYLKKFKALTDKLSAMKKPLDETQTIFHLAR 172
             L  +L  L KGNL+L+EY++KFK L DKL+A+ KP+ +   +F +++
Sbjct: 133 AQLKNSLYALSKGNLSLDEYIRKFKELCDKLTAIGKPVSDVDKVFQISK 181

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154021.19.7e-5465.70uncharacterized protein LOC111021379 [Momordica charantia] >XP_022154022.1 uncha... [more]
RVW19921.13.0e-3948.84Retrovirus-related Pol polyprotein from transposon RE1 [Vitis vinifera][more]
RVW43526.18.8e-3948.26Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera][more]
RVW93768.18.8e-3948.26Retrovirus-related Pol polyprotein from transposon RE1 [Vitis vinifera][more]
GAU44375.11.2e-3851.48hypothetical protein TSUD_243070 [Trifolium subterraneum][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DMG54.7e-5465.70uncharacterized protein LOC111021379 OS=Momordica charantia OX=3673 GN=LOC111021... [more]
A0A438C9J91.5e-3948.84Retrovirus-related Pol polyprotein from transposon RE1 OS=Vitis vinifera OX=2976... [more]
A0A438IAM24.3e-3948.26Retrovirus-related Pol polyprotein from transposon RE1 OS=Vitis vinifera OX=2976... [more]
A0A438E6Z54.3e-3948.26Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX... [more]
A0A2Z6P7T05.6e-3951.48Reverse transcriptase Ty1/copia-type domain-containing protein OS=Trifolium subt... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 125..145
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 75..169
e-value: 7.3E-13
score: 48.5
NoneNo IPR availablePANTHERPTHR47481FAMILY NOT NAMEDcoord: 14..171

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc10g27930.1Moc10g27930.1mRNA