Moc10g15330 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc10g15330
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ribonuclease H
Location	chr10: 11621651 .. 11622238 (-)
RNA-Seq Expression	Moc10g15330
Synteny	Moc10g15330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTATAATGCTGCAGCGAATGGGTTGGCAAAAGCATTCAACAAGACATTATGCAAATTGTTGAAGAAAGTAGTCTCAAAGACAAAAAGAGACTGGCAAGAGAAGATCGGTGAAGTGTTATGGCCTTATCGAACTACTCATCGTACTCCTACAAGAGCTACGCCTTACTCTTTGGTTTATGGTGTAGAAGCTATCTTTCTATTGAAGAGAGAAATTTCATCACTACGAATGGTCGTGCTAGAAGGTTTAACCACTGAAGACAATGCAAAATTACGCCTTCAAGAGTTAGAAGCACTTGATGAAAAGAGATTTGATGCGCAACAAACATTGTAATGCTATCATGCTCAAATCACCAAAGCCTTCAATAAAAATGTTTAGCCTCAATCATTTCAGGCTGATGATATTGTGCTTGCTGTTCGAAGACCTATCATCACGATGCGTCACACAGGGAATAAGTTCACACCTAAATGGGACGAACCTTACATCTTCAAAGAAGTCTACACAAATGGAGCATATAAGATCGTTGATAAAGAAGGATTGAGAATTGGTCCGATTAACGACAAATTTCTTAAAAGATTTTATGCGTGA

mRNA sequence

ATGTATAATGCTGCAGCGAATGGGTTGGCAAAAGCATTCAACAAGACATTATGCAAATTGTTGAAGAAAGTAGTCTCAAAGACAAAAAGAGACTGGCAAGAGAAGATCGGTGAAGTGTTATGGCCTTATCGAACTACTCATCGTACTCCTACAAGAGCTACGCCTTACTCTTTGGTTTATGGTGTAGAAGCTATCTTTCTATTGAAGAGAGAAATTTCATCACTACGAATGGTCGTGCTAGAAGGTTTAACCACTGAAGACAATGCAAAATTACGCCTTCAAGAGTTAGAAGCACTTGATGAAAAGAGATTTGATGCGCAACAAACATTACCTATCATCACGATGCGTCACACAGGGAATAAGTTCACACCTAAATGGGACGAACCTTACATCTTCAAAGAAGTCTACACAAATGGAGCATATAAGATCGTTGATAAAGAAGGATTGAGAATTGGTCCGATTAACGACAAATTTCTTAAAAGATTTTATGCGTGA

Coding sequence (CDS)

ATGTATAATGCTGCAGCGAATGGGTTGGCAAAAGCATTCAACAAGACATTATGCAAATTGTTGAAGAAAGTAGTCTCAAAGACAAAAAGAGACTGGCAAGAGAAGATCGGTGAAGTGTTATGGCCTTATCGAACTACTCATCGTACTCCTACAAGAGCTACGCCTTACTCTTTGGTTTATGGTGTAGAAGCTATCTTTCTATTGAAGAGAGAAATTTCATCACTACGAATGGTCGTGCTAGAAGGTTTAACCACTGAAGACAATGCAAAATTACGCCTTCAAGAGTTAGAAGCACTTGATGAAAAGAGATTTGATGCGCAACAAACATTACCTATCATCACGATGCGTCACACAGGGAATAAGTTCACACCTAAATGGGACGAACCTTACATCTTCAAAGAAGTCTACACAAATGGAGCATATAAGATCGTTGATAAAGAAGGATTGAGAATTGGTCCGATTAACGACAAATTTCTTAAAAGATTTTATGCGTGA

Protein sequence

MYNAAANGLAKAFNKTLCKLLKKVVSKTKRDWQEKIGEVLWPYRTTHRTPTRATPYSLVYGVEAIFLLKREISSLRMVVLEGLTTEDNAKLRLQELEALDEKRFDAQQTLPIITMRHTGNKFTPKWDEPYIFKEVYTNGAYKIVDKEGLRIGPINDKFLKRFYA

Homology

[Close]

BLAST of Moc10g15330 vs. NCBI nr
Match: XP_022151473.1 (uncharacterized protein LOC111019405 [Momordica charantia])

HSP 1 Score: 273.5 bits (698), Expect = 1.2e-69
Identity = 145/195 (74.36%), Postives = 148/195 (75.90%), Query Frame = 0


Query: 1   MYNAAANGLAKAFNKTLCKLLKKVVSKTKRDWQEKIGEVLWPYRTTHRTPTRATPYSLVY 60
           MYNAAANGLA+AFNKTLC LLKKVVSKTKRDWQ+KIGEVLW YRTT RTPTR TPYSLVY
Sbjct: 755 MYNAAANGLAEAFNKTLCNLLKKVVSKTKRDWQKKIGEVLWAYRTTDRTPTRVTPYSLVY 814

Query: 61  GVEAIFLLKREISSLRMVVLEGLTTEDNAKLRLQELEALDEKRFDAQQTL---------- 120
           GVEAI  L+REI SLRM VLEGLTTEDNAKLRLQELEALDEKR DAQQTL          
Sbjct: 815 GVEAILPLEREIPSLRMAVLEGLTTEDNAKLRLQELEALDEKRLDAQQTLECYQARITKA 874

Query: 121 ---------------------PIITMRHTGNKFTPKWDEPYIFKEVYTNGAYKIVDKEGL 165
                                PIIT RHT NKFTPKWD PYI KEVYTNGAY IVDK GL
Sbjct: 875 FNKNVRPRSFQVGDMVLVVRRPIITTRHTRNKFTPKWDGPYIIKEVYTNGAYMIVDKYGL 934

[Close]

BLAST of Moc10g15330 vs. NCBI nr
Match: XP_031739134.1 (uncharacterized protein LOC116402863 [Cucumis sativus])

HSP 1 Score: 268.5 bits (685), Expect = 3.9e-68
Identity = 138/195 (70.77%), Postives = 147/195 (75.38%), Query Frame = 0


Query: 1    MYNAAANGLAKAFNKTLCKLLKKVVSKTKRDWQEKIGEVLWPYRTTHRTPTRATPYSLVY 60
            MYNAAANGLA+AFNKTLC LLKK+VSK+KRDWQEKIGE LW YRTTHRTPT  TPYSLVY
Sbjct: 2237 MYNAAANGLAEAFNKTLCNLLKKIVSKSKRDWQEKIGEALWAYRTTHRTPTGVTPYSLVY 2296

Query: 61   GVEAIFLLKREISSLRMVVLEGLTTEDNAKLRLQELEALDEKRFDAQQTL---------- 120
            GVEA+  L+REI SLRM V EGLTTEDN KLRLQELEALDEKR +AQQ L          
Sbjct: 2297 GVEAVLPLEREIPSLRMAVQEGLTTEDNVKLRLQELEALDEKRLEAQQALECYQARMSKA 2356

Query: 121  ---------------------PIITMRHTGNKFTPKWDEPYIFKEVYTNGAYKIVDKEGL 165
                                 PIIT RHTGNKFTPKWD PYI KEVYTNGAYKIVD++GL
Sbjct: 2357 FDKHVKPRSFQVGDLVLAVRRPIITTRHTGNKFTPKWDGPYIVKEVYTNGAYKIVDQDGL 2416

[Close]

BLAST of Moc10g15330 vs. NCBI nr
Match: XP_031742032.1 (uncharacterized protein LOC116404025 [Cucumis sativus])

HSP 1 Score: 268.5 bits (685), Expect = 3.9e-68
Identity = 138/195 (70.77%), Postives = 147/195 (75.38%), Query Frame = 0


Query: 1    MYNAAANGLAKAFNKTLCKLLKKVVSKTKRDWQEKIGEVLWPYRTTHRTPTRATPYSLVY 60
            MYNAAANGLA+AFNKTLC LLKK+VSK+KRDWQEKIGE LW YRTTHRTPT  TPYSLVY
Sbjct: 2237 MYNAAANGLAEAFNKTLCNLLKKIVSKSKRDWQEKIGEALWAYRTTHRTPTGVTPYSLVY 2296

Query: 61   GVEAIFLLKREISSLRMVVLEGLTTEDNAKLRLQELEALDEKRFDAQQTL---------- 120
            GVEA+  L+REI SLRM V EGLTTEDN KLRLQELEALDEKR +AQQ L          
Sbjct: 2297 GVEAVLPLEREIPSLRMAVQEGLTTEDNVKLRLQELEALDEKRLEAQQALECYQARMSKA 2356

Query: 121  ---------------------PIITMRHTGNKFTPKWDEPYIFKEVYTNGAYKIVDKEGL 165
                                 PIIT RHTGNKFTPKWD PYI KEVYTNGAYKIVD++GL
Sbjct: 2357 FDKHVKPRSFQVGDLVLAVRRPIITTRHTGNKFTPKWDGPYIVKEVYTNGAYKIVDQDGL 2416

[Close]

BLAST of Moc10g15330 vs. NCBI nr
Match: XP_031737372.1 (uncharacterized protein LOC116402244 [Cucumis sativus])

HSP 1 Score: 268.5 bits (685), Expect = 3.9e-68
Identity = 138/195 (70.77%), Postives = 147/195 (75.38%), Query Frame = 0


Query: 1    MYNAAANGLAKAFNKTLCKLLKKVVSKTKRDWQEKIGEVLWPYRTTHRTPTRATPYSLVY 60
            MYNAAANGLA+AFNKTLC LLKK+VSK+KRDWQEKIGE LW YRTTHRTPT  TPYSLVY
Sbjct: 1612 MYNAAANGLAEAFNKTLCNLLKKIVSKSKRDWQEKIGEALWAYRTTHRTPTGVTPYSLVY 1671

Query: 61   GVEAIFLLKREISSLRMVVLEGLTTEDNAKLRLQELEALDEKRFDAQQTL---------- 120
            GVEA+  L+REI SLRM V EGLTTEDN KLRLQELEALDEKR +AQQ L          
Sbjct: 1672 GVEAVLPLEREIPSLRMAVQEGLTTEDNVKLRLQELEALDEKRLEAQQALECYQARMSKA 1731

Query: 121  ---------------------PIITMRHTGNKFTPKWDEPYIFKEVYTNGAYKIVDKEGL 165
                                 PIIT RHTGNKFTPKWD PYI KEVYTNGAYKIVD++GL
Sbjct: 1732 FDKHVKPRSFQVGDLVLAVRRPIITTRHTGNKFTPKWDGPYIVKEVYTNGAYKIVDQDGL 1791

[Close]

BLAST of Moc10g15330 vs. NCBI nr
Match: XP_031742199.1 (uncharacterized protein LOC105435721 [Cucumis sativus])

HSP 1 Score: 268.5 bits (685), Expect = 3.9e-68
Identity = 138/195 (70.77%), Postives = 147/195 (75.38%), Query Frame = 0


Query: 1    MYNAAANGLAKAFNKTLCKLLKKVVSKTKRDWQEKIGEVLWPYRTTHRTPTRATPYSLVY 60
            MYNAAANGLA+AFNKTLC LLKK+VSK+KRDWQEKIGE LW YRTTHRTPT  TPYSLVY
Sbjct: 2096 MYNAAANGLAEAFNKTLCNLLKKIVSKSKRDWQEKIGEALWAYRTTHRTPTGVTPYSLVY 2155

Query: 61   GVEAIFLLKREISSLRMVVLEGLTTEDNAKLRLQELEALDEKRFDAQQTL---------- 120
            GVEA+  L+REI SLRM V EGLTTEDN KLRLQELEALDEKR +AQQ L          
Sbjct: 2156 GVEAVLPLEREIPSLRMAVQEGLTTEDNVKLRLQELEALDEKRLEAQQALECYQARMSKA 2215

Query: 121  ---------------------PIITMRHTGNKFTPKWDEPYIFKEVYTNGAYKIVDKEGL 165
                                 PIIT RHTGNKFTPKWD PYI KEVYTNGAYKIVD++GL
Sbjct: 2216 FDKHVKPRSFQVGDLVLAVRRPIITTRHTGNKFTPKWDGPYIVKEVYTNGAYKIVDQDGL 2275

[Close]

BLAST of Moc10g15330 vs. ExPASy TrEMBL
Match: A0A6J1DC95 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111019405 PE=4 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 5.8e-70
Identity = 145/195 (74.36%), Postives = 148/195 (75.90%), Query Frame = 0


Query: 1   MYNAAANGLAKAFNKTLCKLLKKVVSKTKRDWQEKIGEVLWPYRTTHRTPTRATPYSLVY 60
           MYNAAANGLA+AFNKTLC LLKKVVSKTKRDWQ+KIGEVLW YRTT RTPTR TPYSLVY
Sbjct: 755 MYNAAANGLAEAFNKTLCNLLKKVVSKTKRDWQKKIGEVLWAYRTTDRTPTRVTPYSLVY 814

Query: 61  GVEAIFLLKREISSLRMVVLEGLTTEDNAKLRLQELEALDEKRFDAQQTL---------- 120
           GVEAI  L+REI SLRM VLEGLTTEDNAKLRLQELEALDEKR DAQQTL          
Sbjct: 815 GVEAILPLEREIPSLRMAVLEGLTTEDNAKLRLQELEALDEKRLDAQQTLECYQARITKA 874

Query: 121 ---------------------PIITMRHTGNKFTPKWDEPYIFKEVYTNGAYKIVDKEGL 165
                                PIIT RHT NKFTPKWD PYI KEVYTNGAY IVDK GL
Sbjct: 875 FNKNVRPRSFQVGDMVLVVRRPIITTRHTRNKFTPKWDGPYIIKEVYTNGAYMIVDKYGL 934

[Close]

BLAST of Moc10g15330 vs. ExPASy TrEMBL
Match: A0A5A7U2B8 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G00950 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 5.5e-68
Identity = 136/195 (69.74%), Postives = 147/195 (75.38%), Query Frame = 0


Query: 1    MYNAAANGLAKAFNKTLCKLLKKVVSKTKRDWQEKIGEVLWPYRTTHRTPTRATPYSLVY 60
            MYNAAANGLA+AFNKTLC LLKKVVSKTKRDWQEKIGE LW YRTTHRTPT  TPYSLVY
Sbjct: 1413 MYNAAANGLAEAFNKTLCSLLKKVVSKTKRDWQEKIGEALWAYRTTHRTPTGVTPYSLVY 1472

Query: 61   GVEAIFLLKREISSLRMVVLEGLTTEDNAKLRLQELEALDEKRFDAQQTL---------- 120
            GVE +  L+REI SLRM + EGLTTEDNA+LRLQELEALDEKR +AQQ L          
Sbjct: 1473 GVEVVLPLEREIPSLRMAIQEGLTTEDNARLRLQELEALDEKRLEAQQALECYQARMSKA 1532

Query: 121  ---------------------PIITMRHTGNKFTPKWDEPYIFKEVYTNGAYKIVDKEGL 165
                                 PIIT RHTGNKFTPKWD PYI KEV+TNGAYKI+D++GL
Sbjct: 1533 FDKQVKPRSFQVGDLVLAVRRPIITTRHTGNKFTPKWDGPYIVKEVFTNGAYKIIDQDGL 1592

[Close]

BLAST of Moc10g15330 vs. ExPASy TrEMBL
Match: A0A5D3D1E5 (Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G004020 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 5.5e-68
Identity = 136/195 (69.74%), Postives = 148/195 (75.90%), Query Frame = 0


Query: 1    MYNAAANGLAKAFNKTLCKLLKKVVSKTKRDWQEKIGEVLWPYRTTHRTPTRATPYSLVY 60
            MYNAAANGLA+AFNKTLC LLKKVVSKTKRDWQEKIGE LW YRTTHRTPT  TPYSLVY
Sbjct: 2124 MYNAAANGLAEAFNKTLCSLLKKVVSKTKRDWQEKIGEALWAYRTTHRTPTGVTPYSLVY 2183

Query: 61   GVEAIFLLKREISSLRMVVLEGLTTEDNAKLRLQELEALDEKRFDAQQTL---------- 120
            GVEA+  L+REI SLRM + EGLTTEDNA+LRLQELEALDEKR +AQQ L          
Sbjct: 2184 GVEAVLPLEREIPSLRMAIQEGLTTEDNARLRLQELEALDEKRLEAQQALECYQARMSKA 2243

Query: 121  ---------------------PIITMRHTGNKFTPKWDEPYIFKEVYTNGAYKIVDKEGL 165
                                 PIIT RHTGNKFTPKWD PYI KEV+TNGAYKI+D++GL
Sbjct: 2244 FDKQVRPRSFQVGDLVLAVRRPIITTRHTGNKFTPKWDGPYIVKEVFTNGAYKIIDQDGL 2303

[Close]

BLAST of Moc10g15330 vs. ExPASy TrEMBL
Match: A0A5A7U804 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G001610 PE=4 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 7.1e-68
Identity = 136/195 (69.74%), Postives = 148/195 (75.90%), Query Frame = 0


Query: 1   MYNAAANGLAKAFNKTLCKLLKKVVSKTKRDWQEKIGEVLWPYRTTHRTPTRATPYSLVY 60
           MYNAAANGLA+AFNKTLC LLKKVVSKTKRDWQEKIGE LW YRTTHRTPT  TPYSLVY
Sbjct: 1   MYNAAANGLAEAFNKTLCSLLKKVVSKTKRDWQEKIGEALWAYRTTHRTPTGVTPYSLVY 60

Query: 61  GVEAIFLLKREISSLRMVVLEGLTTEDNAKLRLQELEALDEKRFDAQQTL---------- 120
           GVEA+  L++EI SLRM + EGLTTEDNA+LRLQELEALDEKR +AQQ L          
Sbjct: 61  GVEAVLPLEKEIPSLRMAIQEGLTTEDNARLRLQELEALDEKRLEAQQALECYQARMSKA 120

Query: 121 ---------------------PIITMRHTGNKFTPKWDEPYIFKEVYTNGAYKIVDKEGL 165
                                PIIT RHTGNKFTPKWD PYI KEV+TNGAYKIVD++GL
Sbjct: 121 FNKQVRPRSFQVADLVLAVRRPIITTRHTGNKFTPKWDRPYIIKEVFTNGAYKIVDQDGL 180

[Close]

BLAST of Moc10g15330 vs. ExPASy TrEMBL
Match: A0A5A7TZU9 (Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold498G00940 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 9.3e-68
Identity = 135/195 (69.23%), Postives = 148/195 (75.90%), Query Frame = 0


Query: 1    MYNAAANGLAKAFNKTLCKLLKKVVSKTKRDWQEKIGEVLWPYRTTHRTPTRATPYSLVY 60
            MYNAAANGLA+AFNKTLC LLKKVVSKTKRDWQEKIGE LW YRTTHRTPT  TPYSLVY
Sbjct: 2194 MYNAAANGLAEAFNKTLCSLLKKVVSKTKRDWQEKIGEALWAYRTTHRTPTGVTPYSLVY 2253

Query: 61   GVEAIFLLKREISSLRMVVLEGLTTEDNAKLRLQELEALDEKRFDAQQTL---------- 120
            GVEA+  L+REI SLRM + EGLTTEDNA+LRL+ELEALDEKR +AQQ L          
Sbjct: 2254 GVEAVLPLEREIPSLRMAIQEGLTTEDNARLRLEELEALDEKRLEAQQALECYQARMSKA 2313

Query: 121  ---------------------PIITMRHTGNKFTPKWDEPYIFKEVYTNGAYKIVDKEGL 165
                                 PIIT RHTGNKFTPKWD PYI KEV+TNGAYKI+D++GL
Sbjct: 2314 FDKQVRPRSFQVGDLVLAVRRPIITTRHTGNKFTPKWDGPYIVKEVFTNGAYKIIDQDGL 2373

The following BLAST results are available for this feature:

BLAST of Moc10g15330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151473.1	1.2e-69	74.36	uncharacterized protein LOC111019405 [Momordica charantia]	[more]
XP_031739134.1	3.9e-68	70.77	uncharacterized protein LOC116402863 [Cucumis sativus]	[more]
XP_031742032.1	3.9e-68	70.77	uncharacterized protein LOC116404025 [Cucumis sativus]	[more]
XP_031737372.1	3.9e-68	70.77	uncharacterized protein LOC116402244 [Cucumis sativus]	[more]
XP_031742199.1	3.9e-68	70.77	uncharacterized protein LOC105435721 [Cucumis sativus]	[more]

BLAST of Moc10g15330 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc10g15330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DC95	5.8e-70	74.36	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111019405 PE=4 SV=1	[more]
A0A5A7U2B8	5.5e-68	69.74	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold26...	[more]
A0A5D3D1E5	5.5e-68	69.74	Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G00402...	[more]
A0A5A7U804	7.1e-68	69.74	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A5A7TZU9	9.3e-68	69.23	Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold498G00940...	[more]

BLAST of Moc10g15330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 1..109 e-value: 1.8E-13 score: 52.2
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 5..110
None	No IPR available	PANTHER	PTHR24559:SF342	SUBFAMILY NOT NAMED	coord: 5..110
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 2..66

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc10g15330.1	Moc10g15330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006259	DNA metabolic process
molecular_function	GO:0140640	catalytic activity, acting on a nucleic acid
molecular_function	GO:0003676	nucleic acid binding