Moc10g09750 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc10g09750
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	gibberellin 2-beta-dioxygenase 1-like
Location	chr10: 7352576 .. 7354320 (-)
RNA-Seq Expression	Moc10g09750
Synteny	Moc10g09750

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGGTTTTGTCCAAGCCAGCAATCAAACAGTCTTCTTCTTCTTTGATGATGAGAAACTCCATGGTTACTCCTTCTTCTTCTTCTTCTCCTTCTCCTGCATTTTTCTCCGGAGTTCCATTGATAGACCTCTCGGAACCCGACGCCAAGCAGCGCATCGTCACGGCGTGCGAAGAGCTTGGGTTCTTCAAGGTCGTCAACCATGGCGTCCCCATGGAATTCATCTCCACTCTCGAATCGGAATCCACCAAATTCTTCTCCCTCCCCCTCTCCGAGAAGCAGAAGGCCGGCCCTCCGTCGCCTTTCGGCTATGGTAACAAGCAAATCGGCAGCAATGGCGATGTCGGTCGGGTCGAATACCTCCTCCTAGCCACCCACTTCCAGTCCAACTCCGATGGGTTCCTCTCCCAATTTGGCCAAAACCCACAAAAACTCCGGTACCCCACCAAGCCCCCACCTCTCAAAATGCTCCGTTTTTTCTCTTTACTCTGTTTTCCCCTGTTTTTCCTGACTCTGTTTTTGCTCTGTTTTAGCTCTGCTGTGAACGATTACATCTCGGCCGTGAGGAACATGGCGTGTGAAATCCTGGAACTGATGGCGGAGGGGCTGAAGATTCAGCAGAGGAATGTGTTCAGTAAGCTGTTAATGGACGAAGAGAGCGACTCTGTTTTCAGGCTGAACCATTATCCGCCATGGCTGGAGCTTCACCAAGCTGCTGCTGTGGAAGGGAAGAACATGATTGGATTTGGAGAACACACAGACCCACAGATCATTTCGGTTTTGAGATCCAACAATACTTCTGGACTTCAGATTTCTTTGGCTGATGGAAATTGGATTTCGGTTCCTCCTGATCAGAACTCCTTCTTCATCAATGTTGGTGACTCTTTACAGGTATAGAAAAAAAAAAGAACACAAGACAGTTTCTACAAAAAAAAAAAAAAAAAAACTTCTTTTTTGTGTGTGTTTGTTTATGTCTCTCTCTCTTGGTTTGCCAATCTTTGTACTGTTTTTTATTTATTAAAAACTCCATAGACAAGACATGATCTTAATCATGATTTATGTCTGTCAGCTGGCTTCCTGTTTAGATAAATCTCCCACAAAGCCCACTCTTTCATTAGACTAATATCAAAATTTCTAAAATTTGGGTTTTTTCATTTTTTCTTTTAATGGCAAGAAAAATTTTCAGTTTCTTTCCATTGGGGTTTTACTTTTGCCCTATGTCAAAAGTGGGTTAAAAAGAACAAATTTATTTCTATGTACACTTGCATTTTCCACACTAAAACATTGACTGATATTCAAAGAAACAAAAGGGTTATTCATACAGCCAATTTTCGAGACATGTTTGAATCAGACTGAAAAGTACACACAATAACATGAACTTGGATGTCCCCTATTATTGTTTAATCTAAAAACACATACTTTATTGATTTTAAATATAGGGTTTTTTTTTTGTTCATATATACTAAAAAAATTGAACCTTTTTTGGTTTTTTGAAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGGGTATTGACAAACAGCTTGAAGTCAAGAATTTCAATGATATACTTTGGTGGGCCACCATTGAGTGAGAAGATAGCCCCTTTGCCTTCACTTATGAAAGGAGAAGAAAGTTTGTACAAAGAGTTCACTTGGTTTGAGTACAAAAGATCAGCTTACAATTCCAGGTTGGCAGATAACAGGCTTGTGCACTTTGAAAGAATTGCAGCCTCATAA

mRNA sequence

ATGGTGGTTTTGTCCAAGCCAGCAATCAAACAGTCTTCTTCTTCTTTGATGATGAGAAACTCCATGGTTACTCCTTCTTCTTCTTCTTCTCCTTCTCCTGCATTTTTCTCCGGAGTTCCATTGATAGACCTCTCGGAACCCGACGCCAAGCAGCGCATCGTCACGGCGTGCGAAGAGCTTGGGTTCTTCAAGGTCGTCAACCATGGCGTCCCCATGGAATTCATCTCCACTCTCGAATCGGAATCCACCAAATTCTTCTCCCTCCCCCTCTCCGAGAAGCAGAAGGCCGGCCCTCCGTCGCCTTTCGGCTATGGTAACAAGCAAATCGGCAGCAATGGCGATGTCGGTCGGGTCGAATACCTCCTCCTAGCCACCCACTTCCAGTCCAACTCCGATGGGTTCCTCTCCCAATTTGGCCAAAACCCACAAAAACTCCGCTCTGCTGTGAACGATTACATCTCGGCCGTGAGGAACATGGCGTGTGAAATCCTGGAACTGATGGCGGAGGGGCTGAAGATTCAGCAGAGGAATGTGTTCAGTAAGCTGTTAATGGACGAAGAGAGCGACTCTGTTTTCAGGCTGAACCATTATCCGCCATGGCTGGAGCTTCACCAAGCTGCTGCTGTGGAAGGGAAGAACATGATTGGATTTGGAGAACACACAGACCCACAGATCATTTCGGTTTTGAGATCCAACAATACTTCTGGACTTCAGATTTCTTTGGCTGATGGAAATTGGATTTCGGTTCCTCCTGATCAGAACTCCTTCTTCATCAATGTTGGTGACTCTTTACAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGGGTATTGACAAACAGCTTGAAGTCAAGAATTTCAATGATATACTTTGGTGGGCCACCATTGAGTGAGAAGATAGCCCCTTTGCCTTCACTTATGAAAGGAGAAGAAAGTTTGTACAAAGAGTTCACTTGGTTTGAGTACAAAAGATCAGCTTACAATTCCAGGTTGGCAGATAACAGGCTTGTGCACTTTGAAAGAATTGCAGCCTCATAA

Coding sequence (CDS)

ATGGTGGTTTTGTCCAAGCCAGCAATCAAACAGTCTTCTTCTTCTTTGATGATGAGAAACTCCATGGTTACTCCTTCTTCTTCTTCTTCTCCTTCTCCTGCATTTTTCTCCGGAGTTCCATTGATAGACCTCTCGGAACCCGACGCCAAGCAGCGCATCGTCACGGCGTGCGAAGAGCTTGGGTTCTTCAAGGTCGTCAACCATGGCGTCCCCATGGAATTCATCTCCACTCTCGAATCGGAATCCACCAAATTCTTCTCCCTCCCCCTCTCCGAGAAGCAGAAGGCCGGCCCTCCGTCGCCTTTCGGCTATGGTAACAAGCAAATCGGCAGCAATGGCGATGTCGGTCGGGTCGAATACCTCCTCCTAGCCACCCACTTCCAGTCCAACTCCGATGGGTTCCTCTCCCAATTTGGCCAAAACCCACAAAAACTCCGCTCTGCTGTGAACGATTACATCTCGGCCGTGAGGAACATGGCGTGTGAAATCCTGGAACTGATGGCGGAGGGGCTGAAGATTCAGCAGAGGAATGTGTTCAGTAAGCTGTTAATGGACGAAGAGAGCGACTCTGTTTTCAGGCTGAACCATTATCCGCCATGGCTGGAGCTTCACCAAGCTGCTGCTGTGGAAGGGAAGAACATGATTGGATTTGGAGAACACACAGACCCACAGATCATTTCGGTTTTGAGATCCAACAATACTTCTGGACTTCAGATTTCTTTGGCTGATGGAAATTGGATTTCGGTTCCTCCTGATCAGAACTCCTTCTTCATCAATGTTGGTGACTCTTTACAGGTGATGACTAATGGAAGATTCAAAAGTGTGAAGCATAGGGTATTGACAAACAGCTTGAAGTCAAGAATTTCAATGATATACTTTGGTGGGCCACCATTGAGTGAGAAGATAGCCCCTTTGCCTTCACTTATGAAAGGAGAAGAAAGTTTGTACAAAGAGTTCACTTGGTTTGAGTACAAAAGATCAGCTTACAATTCCAGGTTGGCAGATAACAGGCTTGTGCACTTTGAAAGAATTGCAGCCTCATAA

Protein sequence

MVVLSKPAIKQSSSSLMMRNSMVTPSSSSSPSPAFFSGVPLIDLSEPDAKQRIVTACEELGFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEYLLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNVFSKLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS

Homology

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BLAST of Moc10g09750 vs. NCBI nr
Match: XP_023531222.1 (gibberellin 2-beta-dioxygenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 594.0 bits (1530), Expect = 8.5e-166
Identity = 309/347 (89.05%), Postives = 319/347 (91.93%), Query Frame = 0


Query: 1   MVVLSKPAIKQSSSSLMMRNSMVTPSSSSSPSPAFFSGVPLIDLSEPDAKQRIVTACEEL 60
           MVVLSKPAIKQ +SS  MRNSM    SSS+   AFFSGVPLIDLS PDAK  IV ACEEL
Sbjct: 1   MVVLSKPAIKQMASS-YMRNSMAAAGSSSA---AFFSGVPLIDLSAPDAKHLIVKACEEL 60

Query: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEY 120
           GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQ+AGPPSPFGYGNKQIG NGDVG VEY
Sbjct: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEY 120

Query: 121 LLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNVFS 180
           LLL TH +SNSDGFLS FGQ+PQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRN FS
Sbjct: 121 LLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFS 180

Query: 181 KLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNMIGFGEHTDPQIISVLRSNNTSGLQIS 240
           KLLMDEESDSVFR+NHYPP  EL    A++G NMIGFGEHTDPQIISVLRSNNTSGLQIS
Sbjct: 181 KLLMDEESDSVFRVNHYPPCPELQ---ALKGTNMIGFGEHTDPQIISVLRSNNTSGLQIS 240

Query: 241 LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE 300
           LADGNWISVPPDQ+SFFINVGDSLQVMTNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSE
Sbjct: 241 LADGNWISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSE 300

Query: 301 KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 348
           KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 340

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BLAST of Moc10g09750 vs. NCBI nr
Match: XP_022981800.1 (gibberellin 2-beta-dioxygenase 1-like [Cucurbita maxima])

HSP 1 Score: 593.6 bits (1529), Expect = 1.1e-165
Identity = 308/347 (88.76%), Postives = 319/347 (91.93%), Query Frame = 0


Query: 1   MVVLSKPAIKQSSSSLMMRNSMVTPSSSSSPSPAFFSGVPLIDLSEPDAKQRIVTACEEL 60
           MVVLSKP+IKQ +SS  MRNSM    SSS+   AFFSGVPLIDLS PDAK  IV ACEEL
Sbjct: 1   MVVLSKPSIKQMASS-YMRNSMAAAGSSSA---AFFSGVPLIDLSAPDAKHLIVKACEEL 60

Query: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEY 120
           GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQ+AGPPSPFGYGNKQIG NGDVG VEY
Sbjct: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEY 120

Query: 121 LLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNVFS 180
           LLL TH +S SDGFLS FGQ+PQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRN FS
Sbjct: 121 LLLNTHLESKSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFS 180

Query: 181 KLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNMIGFGEHTDPQIISVLRSNNTSGLQIS 240
           KLLMDEESDSVFR+NHYPP  ELH   A++G NMIGFGEHTDPQIISVLRSNNTSGLQIS
Sbjct: 181 KLLMDEESDSVFRVNHYPPCPELH---ALKGTNMIGFGEHTDPQIISVLRSNNTSGLQIS 240

Query: 241 LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE 300
           LADGNWISVPPDQ+SFFINVGDSLQVMTNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSE
Sbjct: 241 LADGNWISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSE 300

Query: 301 KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 348
           KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 340

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BLAST of Moc10g09750 vs. NCBI nr
Match: KAG6600483.1 (Gibberellin 2-beta-dioxygenase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031125.1 Gibberellin 2-beta-dioxygenase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 592.4 bits (1526), Expect = 2.5e-165
Identity = 308/347 (88.76%), Postives = 318/347 (91.64%), Query Frame = 0


Query: 1   MVVLSKPAIKQSSSSLMMRNSMVTPSSSSSPSPAFFSGVPLIDLSEPDAKQRIVTACEEL 60
           MVVLSKPAIKQ +SS  MRNSM    SSS+   AFFSGVPLIDLS PDAK  IV ACEEL
Sbjct: 1   MVVLSKPAIKQMASS-YMRNSMAAAGSSSA---AFFSGVPLIDLSAPDAKHLIVKACEEL 60

Query: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEY 120
           GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQ+AGPPSPFGYGNKQIG NGDVG VEY
Sbjct: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEY 120

Query: 121 LLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNVFS 180
           LLL TH +SNSDGFLS FGQ+PQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRN FS
Sbjct: 121 LLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFS 180

Query: 181 KLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNMIGFGEHTDPQIISVLRSNNTSGLQIS 240
           KLLMDEESDSVFR+NHYPP  EL     ++G NMIGFGEHTDPQIISVLRSNNTSGLQIS
Sbjct: 181 KLLMDEESDSVFRVNHYPPCPELQ---TLKGTNMIGFGEHTDPQIISVLRSNNTSGLQIS 240

Query: 241 LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE 300
           LADGNWISVPPDQ+SFFINVGDSLQVMTNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSE
Sbjct: 241 LADGNWISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSE 300

Query: 301 KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 348
           KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 340

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BLAST of Moc10g09750 vs. NCBI nr
Match: XP_022942236.1 (gibberellin 2-beta-dioxygenase 1-like [Cucurbita moschata])

HSP 1 Score: 590.9 bits (1522), Expect = 7.2e-165
Identity = 307/347 (88.47%), Postives = 318/347 (91.64%), Query Frame = 0


Query: 1   MVVLSKPAIKQSSSSLMMRNSMVTPSSSSSPSPAFFSGVPLIDLSEPDAKQRIVTACEEL 60
           MVVLS+PAIKQ +SS  MRNSM    SSS+   AFFSGVPLIDLS PDAK  IV ACEEL
Sbjct: 1   MVVLSEPAIKQMASS-YMRNSMAAAGSSSA---AFFSGVPLIDLSAPDAKHLIVKACEEL 60

Query: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEY 120
           GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQ+AGPPSPFGYGNKQIG NGDVG VEY
Sbjct: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEY 120

Query: 121 LLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNVFS 180
           LLL TH +SNSDGFLS FGQ+PQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRN FS
Sbjct: 121 LLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFS 180

Query: 181 KLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNMIGFGEHTDPQIISVLRSNNTSGLQIS 240
           KLLMDEESDSVFR+NHYPP  EL     ++G NMIGFGEHTDPQIISVLRSNNTSGLQIS
Sbjct: 181 KLLMDEESDSVFRVNHYPPCPELQ---TLKGTNMIGFGEHTDPQIISVLRSNNTSGLQIS 240

Query: 241 LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE 300
           LADGNWISVPPDQ+SFFINVGDSLQVMTNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSE
Sbjct: 241 LADGNWISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSE 300

Query: 301 KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 348
           KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 340

[Close]

BLAST of Moc10g09750 vs. NCBI nr
Match: XP_038879745.1 (gibberellin 2-beta-dioxygenase 1-like [Benincasa hispida])

HSP 1 Score: 585.9 bits (1509), Expect = 2.3e-163
Identity = 303/348 (87.07%), Postives = 321/348 (92.24%), Query Frame = 0


Query: 1   MVVLSKPAIKQSSSSLMMRNSMVTPSSSSSPSPAFFSGVPLIDLSEPDAKQRIVTACEEL 60
           MVVLS P+I+Q SS+  MRNS+ + ++++S S AFF+ VPLIDLS PDAKQ IV ACEEL
Sbjct: 1   MVVLSNPSIQQLSSNSFMRNSISSMAAAASSSAAFFTEVPLIDLSAPDAKQLIVKACEEL 60

Query: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEY 120
           GFFKVVNHGVPMEFISTLESEST FFSLPLSEKQKA PPSPFGYGNKQIG NGDVG VEY
Sbjct: 61  GFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEY 120

Query: 121 LLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNVFS 180
           LLL TH +SNSDGFLS FGQ+PQKLRSAVN+YISAVRNMACEILELMAEGLKIQQRNVFS
Sbjct: 121 LLLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFS 180

Query: 181 KLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNMIGFGEHTDPQIISVLRSNNTSGLQIS 240
           KL+MDEESDSVFR+NHYPP  E     A++G+NMIGFGEHTDPQIISVLRSNNTSGLQIS
Sbjct: 181 KLVMDEESDSVFRVNHYPPCPE---PQALKGRNMIGFGEHTDPQIISVLRSNNTSGLQIS 240

Query: 241 LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE 300
           LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE
Sbjct: 241 LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE 300

Query: 301 KIAPLPSLMKGEE-SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 348
           KIAPLPSLMKGEE SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 KIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 345

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BLAST of Moc10g09750 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.2e-125
Identity = 231/347 (66.57%), Postives = 276/347 (79.54%), Query Frame = 0


Query: 1   MVVLSKPAIKQSSSSLMMRNSMVTPSSSSSPSPAFFSGVPLIDLSEPDAKQRIVTACEEL 60
           MV+LSKP  +Q +    +RN+M    SSS         +PL+DLS+PDAK  IV ACE+ 
Sbjct: 1   MVLLSKPTSEQYT---YVRNNMPITFSSS---------IPLVDLSKPDAKTLIVKACEDF 60

Query: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEY 120
           GFFKV+NHG+P++ IS LESE+ KFFSLP +EK+KAGP +PFGYGNK+IG NGD+G +EY
Sbjct: 61  GFFKVINHGIPLDAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIEY 120

Query: 121 LLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNVFS 180
           LLL T    N D   S +G++  K R  + DY  A+RNMACEIL+LMAEGLKIQ +NVFS
Sbjct: 121 LLLTT----NQDHNFSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFS 180

Query: 181 KLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNMIGFGEHTDPQIISVLRSNNTSGLQIS 240
           KL+MD++SD +FR+NHYP   EL    A+ G+N+IGFGEHTDPQIIS+LRSNNTSG QIS
Sbjct: 181 KLVMDKQSDCLFRVNHYPACPEL----AINGENLIGFGEHTDPQIISILRSNNTSGFQIS 240

Query: 241 LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE 300
           L DG+WISVPPD +SFFINVGDSLQVMTNGRFKSV+HRVL N +  R+SMIYF GPPLSE
Sbjct: 241 LRDGSWISVPPDHSSFFINVGDSLQVMTNGRFKSVRHRVLANGIDPRLSMIYFCGPPLSE 300

Query: 301 KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 348
           KIAPLPSLMKG+ESLYKEFTWFEYK S Y SRLADNRL ++ERIAA+
Sbjct: 301 KIAPLPSLMKGKESLYKEFTWFEYKSSTYGSRLADNRLGNYERIAAT 327

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BLAST of Moc10g09750 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 4.9e-116
Identity = 215/347 (61.96%), Postives = 261/347 (75.22%), Query Frame = 0


Query: 1   MVVLSKPAIKQSSSSLMMRNSMVTPSSSSSPSPAFFSGVPLIDLSEPDAKQRIVTACEEL 60
           MVVLS+PA+ Q     +++    TP          F+G+P++DL+ PDAK  IV AC + 
Sbjct: 1   MVVLSQPALNQ---FFLLKPFKSTP---------LFTGIPVVDLTHPDAKNLIVNACRDF 60

Query: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEY 120
           GFFK+VNHGVP+E ++ LE+E+ +FF    SEK +AGPP PFGYG+K+IG NGDVG VEY
Sbjct: 61  GFFKLVNHGVPLELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEY 120

Query: 121 LLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNVFS 180
           LLL T+    S   L  F +NP   R+ V +YI+AV+NM   +LELMAEGL I+QRN  S
Sbjct: 121 LLLNTNPDVISPKSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLS 180

Query: 181 KLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNMIGFGEHTDPQIISVLRSNNTSGLQIS 240
           +LL DE+SDS FRLNHYPP  E+        +N++GFGEHTDPQIISVLRSN+TSGLQI 
Sbjct: 181 RLLKDEKSDSCFRLNHYPPCPEVQAL----NRNLVGFGEHTDPQIISVLRSNSTSGLQIC 240

Query: 241 LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE 300
           L DG W+SVPPDQ SFFINVGD+LQVMTNGRFKSVKHRVL ++ KSR+SMIYFGGP LSE
Sbjct: 241 LTDGTWVSVPPDQTSFFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSE 300

Query: 301 KIAPLPSLM-KGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAA 347
            IAPLPS+M KGEE LYKEFTW EYK++AY SRLADNRL  F++ AA
Sbjct: 301 NIAPLPSVMLKGEECLYKEFTWCEYKKAAYTSRLADNRLAPFQKSAA 331

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BLAST of Moc10g09750 vs. ExPASy Swiss-Prot
Match: Q8LEA2 (Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 SV=2)

HSP 1 Score: 371.3 bits (952), Expect = 1.2e-101
Identity = 188/310 (60.65%), Postives = 229/310 (73.87%), Query Frame = 0


Query: 36  FSGVPLIDLSEPDAKQRIVTACEELGFFKVVNHGVPMEFISTLESESTKFFSLPLSEK-Q 95
           FS +P+ID+S+P++K  +V ACE+ GFFKV+NHGV  E +S LE E+  FFSLP SEK Q
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 96  KAGPPSPFGYGNKQIGSNGDVGRVEYLLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYIS 155
            AG   PFGYGN +IG NGDVG VEYLL+  +  S S        ++P   R+A+ +Y +
Sbjct: 75  VAG--YPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTT 134

Query: 156 AVRNMACEILELMAEGLKIQQRNVFSKLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNM 215
           +VR M  ++LE + +GL I+ RN  SKL+ D+ +DS+ RLNHYPP   L       GKN+
Sbjct: 135 SVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPP-CPLSNKKTNGGKNV 194

Query: 216 IGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKS 275
           IGFGEHTDPQIISVLRSNNTSGLQI+L DG+WISVPPD  SFF NVGDSLQVMTNGRFKS
Sbjct: 195 IGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKS 254

Query: 276 VKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGE-ESLYKEFTWFEYKRSAYNSRL 335
           V+HRVL N  KSR+SMIYF GP L+++IAPL  L+  E E LY+EFTW EYK S YNSRL
Sbjct: 255 VRHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRL 314

Query: 336 ADNRLVHFER 344
           +DNRL  FER
Sbjct: 315 SDNRLQQFER 321

[Close]

BLAST of Moc10g09750 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 6.5e-100
Identity = 193/346 (55.78%), Postives = 248/346 (71.68%), Query Frame = 0


Query: 1   MVVLSKPAIKQSSSSLMMRNSMVTPSSSSSPSPAFFSGVPLIDLSEPDAKQRIVTACEEL 60
           MVVL +P    +  SL+     V   +S S        +P+++L++P+AK RIV ACEE 
Sbjct: 1   MVVLPQPVTLDNHISLIPTYKPVPVLTSHS--------IPVVNLADPEAKTRIVKACEEF 60

Query: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEY 120
           GFFKVVNHGV  E ++ LE E+  FF LP S K +AGPP P+GYGNK+IG NGDVG +EY
Sbjct: 61  GFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEY 120

Query: 121 LLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNVFS 180
           LLL  + Q +S    + F Q PQ  R +V +Y+  ++ ++ ++LE++AE L I+ R+  S
Sbjct: 121 LLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLS 180

Query: 181 KLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNM--IGFGEHTDPQIISVLRSNNTSGLQ 240
           K+L DE+SDS  RLNHYP       AA  E + M  +GFGEHTDPQIISVLRSNNT+GLQ
Sbjct: 181 KMLRDEKSDSCLRLNHYP-------AAEEEAEKMVKVGFGEHTDPQIISVLRSNNTAGLQ 240

Query: 241 ISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPL 300
           I + DG+W++VPPD +SFFINVGD+LQVMTNGRFKSVKHRVL ++ +SRISMIYFGGPPL
Sbjct: 241 ICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPL 300

Query: 301 SEKIAPLPSLMKGEES-LYKEFTWFEYKRSAYNSRLADNRLVHFER 344
           S+KIAPLP L+  ++  LYKEFTW +YK SAY S+L D RL  FE+
Sbjct: 301 SQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEK 331

[Close]

BLAST of Moc10g09750 vs. ExPASy Swiss-Prot
Match: Q8S0S6 (Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX3 PE=1 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.2e-98
Identity = 183/316 (57.91%), Postives = 231/316 (73.10%), Query Frame = 0


Query: 30  SPSPA-FFSGVPLIDLSEPDAKQRIVTACEELGFFKVVNHGVPMEFISTLESESTKFFSL 89
           SP P   FSGVP++DL  P A + +V ACE  GFFKVVNHGV  + +   ESE+ +FFS 
Sbjct: 18  SPDPGDVFSGVPVVDLGSPGAARAVVDACERYGFFKVVNHGVATDTMDKAESEAVRFFSQ 77

Query: 90  PLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEYLLLATHFQSNSDGFLSQFGQNPQKLRSA 149
              +K ++GP  PFGYG+K+IG NGD+G +EYLLLA    S +D        +    R+A
Sbjct: 78  TQPDKDRSGPAYPFGYGSKRIGFNGDMGWLEYLLLALDDASLADACTV---PSCAVFRAA 137

Query: 150 VNDYISAVRNMACEILELMAEGLKIQQRNVFSKLLMDEESDSVFRLNHYPPWLELHQAAA 209
           +N+YIS VR +A  ++E M+EGL I Q +  S L+  E SD VFR+NHYPP   L     
Sbjct: 138 LNEYISGVRKVAVRVMEAMSEGLGIAQADALSALVTAEGSDQVFRVNHYPPCRALQGL-- 197

Query: 210 VEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMT 269
             G ++ GFGEHTDPQ++SVLRSN TSGLQI+L DG W+SVP D++SFF+NVGDSLQV+T
Sbjct: 198 --GCSVTGFGEHTDPQLVSVLRSNGTSGLQIALRDGQWVSVPSDRDSFFVNVGDSLQVLT 257

Query: 270 NGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLM-KGEESLYKEFTWFEYKRS 329
           NGRFKSVKHRV+ NSLKSR+S IYFGGPPL+++IAPLP L+ +GE+SLYKEFTW EYK++
Sbjct: 258 NGRFKSVKHRVVANSLKSRVSFIYFGGPPLAQRIAPLPQLLGEGEQSLYKEFTWDEYKKA 317

Query: 330 AYNSRLADNRLVHFER 344
           AY SRL DNRL  FE+
Sbjct: 318 AYKSRLGDNRLAQFEK 326

[Close]

BLAST of Moc10g09750 vs. ExPASy TrEMBL
Match: A0A6J1J0N2 (gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111480835 PE=3 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 5.4e-166
Identity = 308/347 (88.76%), Postives = 319/347 (91.93%), Query Frame = 0


Query: 1   MVVLSKPAIKQSSSSLMMRNSMVTPSSSSSPSPAFFSGVPLIDLSEPDAKQRIVTACEEL 60
           MVVLSKP+IKQ +SS  MRNSM    SSS+   AFFSGVPLIDLS PDAK  IV ACEEL
Sbjct: 1   MVVLSKPSIKQMASS-YMRNSMAAAGSSSA---AFFSGVPLIDLSAPDAKHLIVKACEEL 60

Query: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEY 120
           GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQ+AGPPSPFGYGNKQIG NGDVG VEY
Sbjct: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEY 120

Query: 121 LLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNVFS 180
           LLL TH +S SDGFLS FGQ+PQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRN FS
Sbjct: 121 LLLNTHLESKSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFS 180

Query: 181 KLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNMIGFGEHTDPQIISVLRSNNTSGLQIS 240
           KLLMDEESDSVFR+NHYPP  ELH   A++G NMIGFGEHTDPQIISVLRSNNTSGLQIS
Sbjct: 181 KLLMDEESDSVFRVNHYPPCPELH---ALKGTNMIGFGEHTDPQIISVLRSNNTSGLQIS 240

Query: 241 LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE 300
           LADGNWISVPPDQ+SFFINVGDSLQVMTNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSE
Sbjct: 241 LADGNWISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSE 300

Query: 301 KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 348
           KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 340

[Close]

BLAST of Moc10g09750 vs. ExPASy TrEMBL
Match: A0A6J1FNB4 (gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447347 PE=3 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 3.5e-165
Identity = 307/347 (88.47%), Postives = 318/347 (91.64%), Query Frame = 0


Query: 1   MVVLSKPAIKQSSSSLMMRNSMVTPSSSSSPSPAFFSGVPLIDLSEPDAKQRIVTACEEL 60
           MVVLS+PAIKQ +SS  MRNSM    SSS+   AFFSGVPLIDLS PDAK  IV ACEEL
Sbjct: 1   MVVLSEPAIKQMASS-YMRNSMAAAGSSSA---AFFSGVPLIDLSAPDAKHLIVKACEEL 60

Query: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEY 120
           GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQ+AGPPSPFGYGNKQIG NGDVG VEY
Sbjct: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQRAGPPSPFGYGNKQIGRNGDVGWVEY 120

Query: 121 LLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNVFS 180
           LLL TH +SNSDGFLS FGQ+PQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRN FS
Sbjct: 121 LLLNTHLESNSDGFLSIFGQDPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNAFS 180

Query: 181 KLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNMIGFGEHTDPQIISVLRSNNTSGLQIS 240
           KLLMDEESDSVFR+NHYPP  EL     ++G NMIGFGEHTDPQIISVLRSNNTSGLQIS
Sbjct: 181 KLLMDEESDSVFRVNHYPPCPELQ---TLKGTNMIGFGEHTDPQIISVLRSNNTSGLQIS 240

Query: 241 LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE 300
           LADGNWISVPPDQ+SFFINVGDSLQVMTNGRFKSVKHRVLTN LKSR+SMIYFGGPPLSE
Sbjct: 241 LADGNWISVPPDQSSFFINVGDSLQVMTNGRFKSVKHRVLTNRLKSRVSMIYFGGPPLSE 300

Query: 301 KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 348
           KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 KIAPLPSLMKGEESLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 340

[Close]

BLAST of Moc10g09750 vs. ExPASy TrEMBL
Match: A0A0A0KXI6 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G075200 PE=3 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 3.3e-163
Identity = 304/348 (87.36%), Postives = 319/348 (91.67%), Query Frame = 0


Query: 1   MVVLSKPAIKQSSSSLMMRNSMVTPSSSSSPSPAFFSGVPLIDLSEPDAKQRIVTACEEL 60
           MV+LSK +I+Q SS+  MRNSM   +S++S SPAF S VPLIDLS PDAKQ IV ACEEL
Sbjct: 1   MVILSKQSIQQLSSNSFMRNSM---ASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEEL 60

Query: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEY 120
           GFFKVVNHGVPMEFISTLESEST FFSLPLSEKQKA PPSPFGYGNKQIG NGDVG VEY
Sbjct: 61  GFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEY 120

Query: 121 LLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNVFS 180
           +LL TH +SNSDGFLS FGQ+PQKLRSAVN+YISAVRNMACEILELMAEGLKIQQRNVFS
Sbjct: 121 ILLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNVFS 180

Query: 181 KLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNMIGFGEHTDPQIISVLRSNNTSGLQIS 240
           KL+MDEESDSVFR+NHYPP     Q  A++G NMIGFGEHTDPQIISVLRSNNTSGLQIS
Sbjct: 181 KLVMDEESDSVFRVNHYPP---CPQIQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQIS 240

Query: 241 LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE 300
           LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE
Sbjct: 241 LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE 300

Query: 301 KIAPLPSLMKGEE-SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 348
           KIAPLPSLMKGEE SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 KIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 342

[Close]

BLAST of Moc10g09750 vs. ExPASy TrEMBL
Match: A0A1S3BRQ7 (gibberellin 2-beta-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103492969 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 7.3e-163
Identity = 304/348 (87.36%), Postives = 318/348 (91.38%), Query Frame = 0


Query: 1   MVVLSKPAIKQSSSSLMMRNSMVTPSSSSSPSPAFFSGVPLIDLSEPDAKQRIVTACEEL 60
           MVVLSK +I+Q SS+  MRNSM + +SSS+   AFFS VPLIDLS PDAKQ IV ACEEL
Sbjct: 1   MVVLSKQSIQQLSSNSFMRNSMASAASSSA---AFFSEVPLIDLSSPDAKQLIVKACEEL 60

Query: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEY 120
           GFFKVVNHGVPMEFISTLESEST FFSLPLSEKQKA PPSPFGYGNKQIG NGDVG VEY
Sbjct: 61  GFFKVVNHGVPMEFISTLESESTNFFSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEY 120

Query: 121 LLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNVFS 180
           +LL TH +SNSDGFLS FGQ+PQKLRSAVN+YISAVRNMACEILELMAEGLKIQQRN FS
Sbjct: 121 ILLNTHLESNSDGFLSIFGQDPQKLRSAVNNYISAVRNMACEILELMAEGLKIQQRNAFS 180

Query: 181 KLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNMIGFGEHTDPQIISVLRSNNTSGLQIS 240
           KL+MDEESDSVFR+NHYPP     Q  A++G NMIGFGEHTDPQIISVLRSNNTSGLQIS
Sbjct: 181 KLVMDEESDSVFRVNHYPP---CPQIQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQIS 240

Query: 241 LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE 300
           LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE
Sbjct: 241 LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE 300

Query: 301 KIAPLPSLMKGEE-SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 348
           KIAPLPSLMKGEE SLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS
Sbjct: 301 KIAPLPSLMKGEERSLYKEFTWFEYKRSAYNSRLADNRLVHFERIAAS 342

[Close]

BLAST of Moc10g09750 vs. ExPASy TrEMBL
Match: S0BDZ9 (GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox1 PE=2 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 2.4e-158
Identity = 290/323 (89.78%), Postives = 301/323 (93.19%), Query Frame = 0


Query: 26  SSSSSPSPAFFSGVPLIDLSEPDAKQRIVTACEELGFFKVVNHGVPMEFISTLESESTKF 85
           +S++S SPAF S VPLIDLS PDAKQ IV ACEELGFFKVVNHGVPMEFISTLESEST F
Sbjct: 2   ASAASSSPAFLSEVPLIDLSSPDAKQLIVKACEELGFFKVVNHGVPMEFISTLESESTNF 61

Query: 86  FSLPLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEYLLLATHFQSNSDGFLSQFGQNPQKL 145
           FSLPLSEKQKA PPSPFGYGNKQIG NGDVG VEY+LL TH +SNSDGFLS FGQ+PQKL
Sbjct: 62  FSLPLSEKQKAAPPSPFGYGNKQIGRNGDVGWVEYILLNTHLESNSDGFLSIFGQDPQKL 121

Query: 146 RSAVNDYISAVRNMACEILELMAEGLKIQQRNVFSKLLMDEESDSVFRLNHYPPWLELHQ 205
           RSAVN+YISAVRNMACEILELMAEGLKIQQRNVFSKL+MDEESDSVFR+NHYPP     Q
Sbjct: 122 RSAVNNYISAVRNMACEILELMAEGLKIQQRNVFSKLVMDEESDSVFRVNHYPP---CPQ 181

Query: 206 AAAVEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQ 265
             A++G NMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQ
Sbjct: 182 IQALKGMNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQ 241

Query: 266 VMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEE-SLYKEFTWFEY 325
           VMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEE SLYKEFTWFEY
Sbjct: 242 VMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGEERSLYKEFTWFEY 301

Query: 326 KRSAYNSRLADNRLVHFERIAAS 348
           KRSAYNSRLADNRLVHFERIAAS
Sbjct: 302 KRSAYNSRLADNRLVHFERIAAS 321

[Close]

BLAST of Moc10g09750 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 371.3 bits (952), Expect = 8.4e-103
Identity = 188/310 (60.65%), Postives = 229/310 (73.87%), Query Frame = 0


Query: 36  FSGVPLIDLSEPDAKQRIVTACEELGFFKVVNHGVPMEFISTLESESTKFFSLPLSEK-Q 95
           FS +P+ID+S+P++K  +V ACE+ GFFKV+NHGV  E +S LE E+  FFSLP SEK Q
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 96  KAGPPSPFGYGNKQIGSNGDVGRVEYLLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYIS 155
            AG   PFGYGN +IG NGDVG VEYLL+  +  S S        ++P   R+A+ +Y +
Sbjct: 75  VAG--YPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTT 134

Query: 156 AVRNMACEILELMAEGLKIQQRNVFSKLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNM 215
           +VR M  ++LE + +GL I+ RN  SKL+ D+ +DS+ RLNHYPP   L       GKN+
Sbjct: 135 SVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPP-CPLSNKKTNGGKNV 194

Query: 216 IGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKS 275
           IGFGEHTDPQIISVLRSNNTSGLQI+L DG+WISVPPD  SFF NVGDSLQVMTNGRFKS
Sbjct: 195 IGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKS 254

Query: 276 VKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLMKGE-ESLYKEFTWFEYKRSAYNSRL 335
           V+HRVL N  KSR+SMIYF GP L+++IAPL  L+  E E LY+EFTW EYK S YNSRL
Sbjct: 255 VRHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRL 314

Query: 336 ADNRLVHFER 344
           +DNRL  FER
Sbjct: 315 SDNRLQQFER 321

[Close]

BLAST of Moc10g09750 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 365.5 bits (937), Expect = 4.6e-101
Identity = 193/346 (55.78%), Postives = 248/346 (71.68%), Query Frame = 0


Query: 1   MVVLSKPAIKQSSSSLMMRNSMVTPSSSSSPSPAFFSGVPLIDLSEPDAKQRIVTACEEL 60
           MVVL +P    +  SL+     V   +S S        +P+++L++P+AK RIV ACEE 
Sbjct: 1   MVVLPQPVTLDNHISLIPTYKPVPVLTSHS--------IPVVNLADPEAKTRIVKACEEF 60

Query: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEY 120
           GFFKVVNHGV  E ++ LE E+  FF LP S K +AGPP P+GYGNK+IG NGDVG +EY
Sbjct: 61  GFFKVVNHGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEY 120

Query: 121 LLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNVFS 180
           LLL  + Q +S    + F Q PQ  R +V +Y+  ++ ++ ++LE++AE L I+ R+  S
Sbjct: 121 LLLNANPQLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLS 180

Query: 181 KLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNM--IGFGEHTDPQIISVLRSNNTSGLQ 240
           K+L DE+SDS  RLNHYP       AA  E + M  +GFGEHTDPQIISVLRSNNT+GLQ
Sbjct: 181 KMLRDEKSDSCLRLNHYP-------AAEEEAEKMVKVGFGEHTDPQIISVLRSNNTAGLQ 240

Query: 241 ISLADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPL 300
           I + DG+W++VPPD +SFFINVGD+LQVMTNGRFKSVKHRVL ++ +SRISMIYFGGPPL
Sbjct: 241 ICVKDGSWVAVPPDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPL 300

Query: 301 SEKIAPLPSLMKGEES-LYKEFTWFEYKRSAYNSRLADNRLVHFER 344
           S+KIAPLP L+  ++  LYKEFTW +YK SAY S+L D RL  FE+
Sbjct: 301 SQKIAPLPCLVPEQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEK 331

[Close]

BLAST of Moc10g09750 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 329.7 bits (844), Expect = 2.8e-90
Identity = 177/344 (51.45%), Postives = 229/344 (66.57%), Query Frame = 0


Query: 1   MVVLSKPAIKQSSSSLMMRNSMVTPSSSSSPSPAFFSGVPLIDLSEPDAKQRIVTACEEL 60
           MV++ +PA   S       N  V P     P P     +P+IDL++ DAK +IV ACEE 
Sbjct: 1   MVIVLQPASFDS-------NLYVNP--KCKPRPVL---IPVIDLTDSDAKTQIVKACEEF 60

Query: 61  GFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEY 120
           GFFKV+NHGV  + ++ LE E+  FF+L  S K KAGPP PFGYG K+IG NGD+G +EY
Sbjct: 61  GFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEY 120

Query: 121 LLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNVFS 180
           +LL  +    S    + F   P   R AV +YI  ++ M+ + LE++ E LKI+ +   S
Sbjct: 121 ILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLS 180

Query: 181 KLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNMIGFGEHTDPQIISVLRSNNTSGLQIS 240
           +L+  +ESDS  R+NHYP      +      K  IGFGEHTDPQ+IS+LRSN+T GLQI 
Sbjct: 181 RLVKVKESDSCLRMNHYP------EKEETPVKEEIGFGEHTDPQLISLLRSNDTEGLQIC 240

Query: 241 LADGNWISVPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKSRISMIYFGGPPLSE 300
           + DG W+ V PD +SFF+ VGD+LQVMTNGRFKSVKHRV+TN+ +SRISMIYF GPPLSE
Sbjct: 241 VKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSE 300

Query: 301 KIAPLPSLM-KGEESLYKEFTWFEYKRSAYNSRLADNRLVHFER 344
           KIAPL  L+ K ++ LY EFTW +YK SAY ++L D RL  FE+
Sbjct: 301 KIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGLFEK 326

[Close]

BLAST of Moc10g09750 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 285.0 bits (728), Expect = 7.9e-77
Identity = 161/338 (47.63%), Postives = 208/338 (61.54%), Query Frame = 0


Query: 22  MVTPSSS----------SSPSPAFFSGVPLIDLSEPD---AKQRIVTACEELGFFKVVNH 81
           MV PSS+          SSP   F    P+ID S  D     ++IV ACE  GFFKV+NH
Sbjct: 1   MVLPSSTPLQTTGKKTISSPEYNF----PVIDFSLNDRSKLSEKIVKACEVNGFFKVINH 60

Query: 82  GVPMEFISTLESESTKFFSLPLSEKQKAGPPSPFGYGNKQIGSNGDVGRVEYLLLATHFQ 141
           GV  E I   E E  +FF+ P S+K +AGP SPFGYG K IG NGD+G +EYLLL  +  
Sbjct: 61  GVKPEIIKRFEHEGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPT 120

Query: 142 SNSDGFLSQFGQNPQKLRSAVNDYISAVRNMACEILELMAEGLKIQQRNVFSKLLMDEES 201
           + +D   +    +P K  SA NDYI  VR++ACEI++L  E L  Q+ +  S+L+ D  S
Sbjct: 121 AVADKSETISHDDPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRS 180

Query: 202 DSVFRLNHYPPWLELHQAAAVEGKNMIGFGEHTDPQIISVLRSNNTSGLQISLADGNWIS 261
           DS+ RLNHYPP        A+ G   IGFGEH+DPQI++VLRSN+  GL+I   DG WI 
Sbjct: 181 DSILRLNHYPP-----APYALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIP 240

Query: 262 VPPDQNSFFINVGDSLQVMTNGRFKSVKHRVLTNSLKS-RISMIYFGGPPLSEKIAPLPS 321
           +P D   FF+ VGD LQ +TNGRF SV+HRVL N+ K  R+S +YF  PPL  KI+PLP 
Sbjct: 241 IPSDPTCFFVLVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPK 300

Query: 322 LMKGEE-SLYKEFTWFEYKRSAYNSRLADNRLVHFERI 345
           ++  E    Y  FTW +YK++ Y+ RL   RL  F+ +
Sbjct: 301 MVSPENPRRYNSFTWGDYKKATYSLRLDVPRLEFFKTL 329

[Close]

BLAST of Moc10g09750 vs. TAIR 10
Match: AT1G47990.1 (gibberellin 2-oxidase 4 )

HSP 1 Score: 284.3 bits (726), Expect = 1.3e-76
Identity = 156/312 (50.00%), Postives = 202/312 (64.74%), Query Frame = 0


Query: 39  VPLIDLSE--PDAKQRIVTACEELGFFKVVNHGVPMEFISTLESESTKFFSLPLSEKQKA 98
           +P+ID+S+       +IV ACE LGFFKV+NHGV    IS +E ES  FF+ P  EK+  
Sbjct: 15  IPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFFAKPAHEKKSV 74

Query: 99  GPPS-PFGYGNKQIGSNGDVGRVEYLLLATHFQSNSDGFLSQFGQNPQKLRSAVNDYISA 158
            P + PF YG + IG NGD G VEYLL    F +N   F SQ         SAVN YI A
Sbjct: 75  RPVNQPFRYGFRDIGLNGDSGEVEYLL----FHTNDPAFRSQL-----SFSSAVNCYIEA 134

Query: 159 VRNMACEILELMAEGLKIQQRNVFSKLLMDEESDSVFRLNHYPPWLELHQAAAVEGKNM- 218
           V+ +A EIL+L AEGL +   + FS+L+   +SDSV R+NHYPP  +    A +  +++ 
Sbjct: 135 VKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFGEANLSDQSVS 194

Query: 219 ---IGFGEHTDPQIISVLRSNNTSGLQISLADGNWISVPPDQNSFFINVGDSLQVMTNGR 278
              +GFGEHTDPQI++VLRSN   GLQ+S +DG W+SV PD ++F +NVGD LQVMTNGR
Sbjct: 195 LTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVNVGDLLQVMTNGR 254

Query: 279 FKSVKHRVLTNSLKSRISMIYFGGPPLSEKIAPLPSLM--KGEESLYKEFTWFEYKRSAY 338
           F SV+HR LT   +SR+S  YF GPPL  KI PL +++    +  LY+ FTW EYK+ AY
Sbjct: 255 FISVRHRALTYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLYQTFTWGEYKKRAY 314

Query: 339 NSRLADNRLVHF 342
           + RL D+RL  F
Sbjct: 315 SLRLEDSRLDMF 316

The following BLAST results are available for this feature:

BLAST of Moc10g09750 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023531222.1	8.5e-166	89.05	gibberellin 2-beta-dioxygenase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022981800.1	1.1e-165	88.76	gibberellin 2-beta-dioxygenase 1-like [Cucurbita maxima]	[more]
KAG6600483.1	2.5e-165	88.76	Gibberellin 2-beta-dioxygenase 1, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022942236.1	7.2e-165	88.47	gibberellin 2-beta-dioxygenase 1-like [Cucurbita moschata]	[more]
XP_038879745.1	2.3e-163	87.07	gibberellin 2-beta-dioxygenase 1-like [Benincasa hispida]	[more]

BLAST of Moc10g09750 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SQ80	1.2e-125	66.57	Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1	[more]
Q9XG83	4.9e-116	61.96	Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=...	[more]
Q8LEA2	1.2e-101	60.65	Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 ...	[more]
Q9XFR9	6.5e-100	55.78	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
Q8S0S6	1.2e-98	57.91	Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]

BLAST of Moc10g09750 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J0N2	5.4e-166	88.76	gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11148083...	[more]
A0A6J1FNB4	3.5e-165	88.47	gibberellin 2-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111447...	[more]
A0A0A0KXI6	3.3e-163	87.36	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G...	[more]
A0A1S3BRQ7	7.3e-163	87.36	gibberellin 2-beta-dioxygenase 1 OS=Cucumis melo OX=3656 GN=LOC103492969 PE=3 SV...	[more]
S0BDZ9	2.4e-158	89.78	GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox1 PE=2 SV=1	[more]

BLAST of Moc10g09750 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G78440.1	8.4e-103	60.65	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]
AT1G30040.1	4.6e-101	55.78	gibberellin 2-oxidase	[more]
AT2G34555.1	2.8e-90	51.45	gibberellin 2-oxidase 3	[more]
AT1G02400.1	7.9e-77	47.63	gibberellin 2-oxidase 6	[more]
AT1G47990.1	1.3e-76	50.00	gibberellin 2-oxidase 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 256..282 score: 33.0 coord: 53..70 score: 53.03 coord: 220..241 score: 32.23
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 31..344
None	No IPR available	PANTHER	PTHR47990:SF150	GIBBERELLIN 2-BETA-DIOXYGENASE 1	coord: 31..344
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 33..335
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 6..335 e-value: 7.5E-78 score: 264.1
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 189..294 e-value: 2.1E-25 score: 89.1
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 39..108 e-value: 1.1E-15 score: 58.4
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 187..296 score: 12.137236

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc10g09750.1	Moc10g09750.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045487	gibberellin catabolic process
biological_process	GO:0009416	response to light stimulus
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0052634	C-19 gibberellin 2-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding