Moc10g04530 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc10g04530
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionsodium/proton antiporter 1
Locationchr10: 3141045 .. 3145615 (-)
RNA-Seq ExpressionMoc10g04530
SyntenyMoc10g04530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCCTCTCCATTGGGATTCACTTGCCCCCATCGCATCATTGGAAGAGATTTTCACTTCAGTCCCTTTCTCCTATTTCACTCCCATTTCACACTCGCTACGCTTCTTCTTCGAGCAGGCTCAGAACTCCAAGGTTGCTCCGTAAGAACCTTCTCGCGCGGGCTGAAGACGAGGCAAAAGGCTCTTCACCAGCTTCGTCTTCTTCATTTCAGCAACAGCCAGCTCCACCCGGCTTCAGTAATCAACTCGAGGTTTCCCTTTTTGAAACTCTTGCTGAATGTTTCGAACTGTTTGTTGTAGGATTCTGGTATTTCGTAAAAGGGTACATAGAAATTTGATCGATAATGCTTTCAATCTTTCATTTGTAATCTCATTTTGATTCGTGAAGTATGGTATATTCATTCTCTACTACTGTTATTGTCGTCTGAATGATGAAGATGTTATCACTTATCAGTATACCATGTCTAAAGCTTTGAGCTGGTAAGGAATTTATTTGTGTTCAACTCTGGCATAATATCAGGAATTCACATCAAAGTCAGGAACCTGTGATCCCCTATGCTCAGTTGACGAGATAAGTTCACAAGATTTCGAAGCTAGTTACCAGCCGAAGACCGATTTACTAAAAGCTTTTGCAATATTTGCTGCAGCCATTACTGGGACAGTGGCAATTAACCACTCCTGGGTTGCTGCTAATCAGGTTCATATGTTTGGTCTTGTCTGATTCTTTCTTTTAGTTTTTGTGTCTTGTTCTGAACTATATATATATCTAGTAACAAGTAACAAGTGCAGTATGCTTGATATGTGTCCTTAATCTTTTTATCCAATTTGTGTTACAGGATCTTGCTATGGTGTTGTTGTTTAGTATAGGATATGCTGGAATCATTTTTGAAGAATCTCTTGCCTTCAACAAGAGTGGAGTGGGATTATTGATGGCTGTCGGATTATGGGTTGTTCGAAGTATTGGGGTAAGATTCAGTTTCTATATAAAACGTTCCGATACATGCCCGTTTTACTTGGGATGTTTGTTTGAGTTTGATTATAGAACTTAAAATTTGTTTGTTGCTTTTCTACTACTTTCATTTTCTTTACTACCTGGAGAAAATCTATAGATTTCTGGTAGAGATGTTTTCATATTGTATAAGCTTCGGTTTTATCTCGTAACTTAATAGTTAATGAACATTCCAATGAAAGAAATCTCGCGTGACTCATTCTATGTAATGTGATAATTTATTTTAGTTCGTGTTTGACGAACTTATTCAGCTGATTTGATGGATCTATTTTATGCTTTCATCGGTGTTATTAATCAACAGAATAGCAATTTATGGTTAGTAATACACACTTCTTTGTTTCTCTCCTCTATCTGGATTCTCCTGCATTTTCTTCATGAGCATTAGAATTCCATGGTCAACAATCGACAATCTCGTAGACATTTTCTACATTCTCCATCCAAAACGCAAATGGAAGATCATAGCGGCTGTTACATTACTCATCTTTCAAAATGGCAAAAGCATACCAAAAATATTACTTTAAACTGCATTTCTCGAACGACAACTGATTCATTTTTAGCATCCAATTGCTTTAAGAATAATCACTCAAAAGTCGATTCACCGTATATGCTGTATGCCATAAATTACGATAGGGAGAGTGGAGTTTGCTTCTACCTTGGTTGCAATCCTCCATGGCGTTGCTGCCTTTTCTAGCATATTGAAGTTTTATTTGTTTGCTTTGTGCAGTCATTCAGTTACTTCTTATTATAAATTCATATGTAGGCTCCATCCACTGAAATTGCAGTTTCAGAGCTAACACATTCAACAGCAGAAGTCAGTGAAATTGTATTTTTCTTGCTTGGGGCAATGACCATAGTAGAAATCGTCGATGCTCATCAAGGATTTAAGTTGGTTACAGACAGTATAACCACTCGAAAACCCCGAACGCTCCTCTGGGTGGTCAGTGTTCTCCTTTTCCTTCCCAAAAAGTATATAGATCTTGATGTGTTTTATTTAAATTCTTTTCCAAACGTAAGGGTCTTGACTTTTGTCCATTACTTGATTGTTAAACTCACCTACATGTTTCAATTAAATTGGTAGACGTGAAAAAAAATCTCAGGCGATCGATGAATCGAAATGAAGGAAAATATTCCGAACATTTTGTTTGTTTTGTCTATACTGGTTGCCTTTTCATATGGGGAAGACAGATCTGTTTCCTTCTTATTTACTTGAGACCCTCGAGCTAGTTGTACTAATCTCTACATTCTTTGACTTACTATACTGAAAAAGCACAGCTAAAATCCTTTTATTGCAGATTGGAGGTGTAACCTTTTTTCTAAGTTCGATCCTCGACAACCTGACAGCCACAATTATCATGGTTTCTCTATTGAGAAAACTAGTTCCTCCATCAGAGTATCGCAAGTATGATACCGATAATGCACAACTTATACATTCGTGATTGCTCTATTATACAATTAAGTGGCTGTACATGTCTAATATATAGCACTCTGTGTAAAATACTGGTTCATGTTGTTGAGGCGCACATGATAGGGTCAACAAGCCTCATATAAGCATACGTATTCTAGGAATAAGAAGTGTGGGAATGAGCGTGACTAGTTCGTGTGTTAGAAAGGGGAACTGGGACCCTCCTGGTATGGTTAGAAAAGTGTAAATAGATGATGCAAGTAAGCAAATCAGAAAATCACTAAACTAGTTGAGGGAGAGACGAAGACCTCTTGAACAGTCTGAAATCTGGTATTTCTCCTCTTATTTTGCTTACCTACAGTTTAGTAACCCCAGCTGTCATATCAGCACAATAATATGTAGCTTTGCAGATGTTAGTTGTCTGTATACTCTATCTATTGATCATAAAACTAGATTTACATGGAGAGTGCAAAATGCAATTCTCGTCTAATGTCTAATTCGATGCATTCGATCGTCCAGTATTTAGAAAGTTCTGTTAGTTCCTTTTCTGGAATTGGCCAAACCATTGGTTTCCAACCAATTCTGTGATCTCAATTTAAGAAACGGATCATACGAAACAGGCTACTTGGAGCTGTGATTGTGATAGCAGCAAATGCCGGTGGAGCATGGACACCGATTGGAGATGTAACCACCACCATGCTGTGGATACATGGTCAGATATCAACTGTGCAGACAGTCAAAGTACTATTCTATCTCTGAAATACTTATTGTGGTTCTTTCTTATGAATGATGAATTTATTCTGATCTGAAATGCTTATTGGTGTTTGGGGTTGGGAGATTTTTGGTAATAGTTTGTGGAAGAGTCTGGACTTCTTGAAGTTCTTGGAAATCTTGTAATTTTACTTCTTTATAGCAGTTCTATTTTCTGTTCATCTATATATCAATCGTTGTCCATCTTGTTCGCAGGACTTGGTTGTACCATCAGCTGTTTCGCTTGCAGTTCCACTGGCTCTTCTGTCTCTAACAAGGTGTAGATTTTGCACCTAAGTCAAAGTTATGGTTTTGTGAAAGTCGAGATTTCTTTTGAGGAATTCTGTTAAGGTTGAACTTATTTGCAAGTTTTTCTCTTCCTGGATTCAGTGAAGTCAATGTGAAGGGGCAGGAATTTCCTAATGTCATGGCATCCGAGCAGATGGCGCCTCGAGGAAAGCTTGTTTTCTCTGTAGGTGTCGGAGCTTTGGTCTTTGTTCCAGTGTTCAAAGCCCTCACAGGTTTGCCCCCCTACATGGGTATGCTGCTTGGGCTTGGAATTCTTTGGATTCTCACTGATGCAATTCACTATGGCGAATCGGAAAGGCAAAAGTTAAAAGTTCCTCAGGCTTTATCTCGTATCGATACGCAGGGAGCGCTTTTCTTCCTTGGAATCCTTTTGTCTGTCAGCAGGTGAGTGCACCATCTACCATCCATTCGAGATTTTATACCATTTCTTGTACTTCCAGTGTGCAATATGCAACATAACAACTTTGCAACATAACAACTTAATCCTTGATCTGAAATGTTTATTCTGATCTGAACTTGAATTTTTATATCAAGAATGCAATGCCTTATTTACATTGCATCTATTGAAGCAAGCCTGAATTTTCATTTCCAGCTTAGAGGCTGCAGGGATCTTACGCGAGTTAGCAAACTACCTCGATGCTCATATTGCGAATGCTGAACTCATTGCAAGTGCAGTTGGTGTTGTATCAGCAATAATAGACAATGTACCGCTAGTTGCCGCAACAATGGGAATGTATGATCTATCCTCCTTCCCTCGAGATTCCGAGTTCTGGCAACTGATTGCATTTTGTGCTGGTACTGGTGGATCCATGCTCATAATTGGATCTGCAGCTGGAGTTGCATTCATGGGGATGGAGAAGGTGGATTTCTTTTGGTACCTAAGAAAGGTGATGCCCCTGTTTCCACATTCAGCAGATGAAACGATAATCCGATTTCTAAGAATTGAGTGTTTTATTCGTCTATTTCTTCTTCTGCAGGTCAGTGGTTTCGCATTTGCAGGGTATGCTGCTGGAATTGCTGCCTATTTAGCTCTTCATAACCTCAACATATCGTTACCAACAACTATTGCTGAGGTGCCCTTTATTTTGGGTTCATAA

mRNA sequence

ATGGCTTCCCTCTCCATTGGGATTCACTTGCCCCCATCGCATCATTGGAAGAGATTTTCACTTCAGTCCCTTTCTCCTATTTCACTCCCATTTCACACTCGCTACGCTTCTTCTTCGAGCAGGCTCAGAACTCCAAGGTTGCTCCGTAAGAACCTTCTCGCGCGGGCTGAAGACGAGGCAAAAGGCTCTTCACCAGCTTCGTCTTCTTCATTTCAGCAACAGCCAGCTCCACCCGGCTTCAGTAATCAACTCGAGGAATTCACATCAAAGTCAGGAACCTGTGATCCCCTATGCTCAGTTGACGAGATAAGTTCACAAGATTTCGAAGCTAGTTACCAGCCGAAGACCGATTTACTAAAAGCTTTTGCAATATTTGCTGCAGCCATTACTGGGACAGTGGCAATTAACCACTCCTGGGTTGCTGCTAATCAGGATCTTGCTATGGTGTTGTTGTTTAGTATAGGATATGCTGGAATCATTTTTGAAGAATCTCTTGCCTTCAACAAGAGTGGAGTGGGATTATTGATGGCTGTCGGATTATGGGTTGTTCGAAGTATTGGGGCTCCATCCACTGAAATTGCAGTTTCAGAGCTAACACATTCAACAGCAGAAGTCAGTGAAATTGTATTTTTCTTGCTTGGGGCAATGACCATAGTAGAAATCGTCGATGCTCATCAAGGATTTAAGTTGGTTACAGACAGTATAACCACTCGAAAACCCCGAACGCTCCTCTGGGTGATTGGAGGTGTAACCTTTTTTCTAAGTTCGATCCTCGACAACCTGACAGCCACAATTATCATGGTTTCTCTATTGAGAAAACTAGTTCCTCCATCAGAGTATCGCAAGCTACTTGGAGCTGTGATTGTGATAGCAGCAAATGCCGGTGGAGCATGGACACCGATTGGAGATGTAACCACCACCATGCTGTGGATACATGGTCAGATATCAACTGTGCAGACAGTCAAAGACTTGGTTGTACCATCAGCTGTTTCGCTTGCAGTTCCACTGGCTCTTCTGTCTCTAACAAGTGAAGTCAATGTGAAGGGGCAGGAATTTCCTAATGTCATGGCATCCGAGCAGATGGCGCCTCGAGGAAAGCTTGTTTTCTCTGTAGGTGTCGGAGCTTTGGTCTTTGTTCCAGTGTTCAAAGCCCTCACAGGTTTGCCCCCCTACATGGGTATGCTGCTTGGGCTTGGAATTCTTTGGATTCTCACTGATGCAATTCACTATGGCGAATCGGAAAGGCAAAAGTTAAAAGTTCCTCAGGCTTTATCTCGTATCGATACGCAGGGAGCGCTTTTCTTCCTTGGAATCCTTTTGTCTGTCAGCAGCTTAGAGGCTGCAGGGATCTTACGCGAGTTAGCAAACTACCTCGATGCTCATATTGCGAATGCTGAACTCATTGCAAGTGCAGTTGGTGTTGTATCAGCAATAATAGACAATGTACCGCTAGTTGCCGCAACAATGGGAATGTATGATCTATCCTCCTTCCCTCGAGATTCCGAGTTCTGGCAACTGATTGCATTTTGTGCTGGTACTGGTGGATCCATGCTCATAATTGGATCTGCAGCTGGAGTTGCATTCATGGGGATGGAGAAGGTGGATTTCTTTTGGTACCTAAGAAAGGTCAGTGGTTTCGCATTTGCAGGGTATGCTGCTGGAATTGCTGCCTATTTAGCTCTTCATAACCTCAACATATCGTTACCAACAACTATTGCTGAGGTGCCCTTTATTTTGGGTTCATAA

Coding sequence (CDS)

ATGGCTTCCCTCTCCATTGGGATTCACTTGCCCCCATCGCATCATTGGAAGAGATTTTCACTTCAGTCCCTTTCTCCTATTTCACTCCCATTTCACACTCGCTACGCTTCTTCTTCGAGCAGGCTCAGAACTCCAAGGTTGCTCCGTAAGAACCTTCTCGCGCGGGCTGAAGACGAGGCAAAAGGCTCTTCACCAGCTTCGTCTTCTTCATTTCAGCAACAGCCAGCTCCACCCGGCTTCAGTAATCAACTCGAGGAATTCACATCAAAGTCAGGAACCTGTGATCCCCTATGCTCAGTTGACGAGATAAGTTCACAAGATTTCGAAGCTAGTTACCAGCCGAAGACCGATTTACTAAAAGCTTTTGCAATATTTGCTGCAGCCATTACTGGGACAGTGGCAATTAACCACTCCTGGGTTGCTGCTAATCAGGATCTTGCTATGGTGTTGTTGTTTAGTATAGGATATGCTGGAATCATTTTTGAAGAATCTCTTGCCTTCAACAAGAGTGGAGTGGGATTATTGATGGCTGTCGGATTATGGGTTGTTCGAAGTATTGGGGCTCCATCCACTGAAATTGCAGTTTCAGAGCTAACACATTCAACAGCAGAAGTCAGTGAAATTGTATTTTTCTTGCTTGGGGCAATGACCATAGTAGAAATCGTCGATGCTCATCAAGGATTTAAGTTGGTTACAGACAGTATAACCACTCGAAAACCCCGAACGCTCCTCTGGGTGATTGGAGGTGTAACCTTTTTTCTAAGTTCGATCCTCGACAACCTGACAGCCACAATTATCATGGTTTCTCTATTGAGAAAACTAGTTCCTCCATCAGAGTATCGCAAGCTACTTGGAGCTGTGATTGTGATAGCAGCAAATGCCGGTGGAGCATGGACACCGATTGGAGATGTAACCACCACCATGCTGTGGATACATGGTCAGATATCAACTGTGCAGACAGTCAAAGACTTGGTTGTACCATCAGCTGTTTCGCTTGCAGTTCCACTGGCTCTTCTGTCTCTAACAAGTGAAGTCAATGTGAAGGGGCAGGAATTTCCTAATGTCATGGCATCCGAGCAGATGGCGCCTCGAGGAAAGCTTGTTTTCTCTGTAGGTGTCGGAGCTTTGGTCTTTGTTCCAGTGTTCAAAGCCCTCACAGGTTTGCCCCCCTACATGGGTATGCTGCTTGGGCTTGGAATTCTTTGGATTCTCACTGATGCAATTCACTATGGCGAATCGGAAAGGCAAAAGTTAAAAGTTCCTCAGGCTTTATCTCGTATCGATACGCAGGGAGCGCTTTTCTTCCTTGGAATCCTTTTGTCTGTCAGCAGCTTAGAGGCTGCAGGGATCTTACGCGAGTTAGCAAACTACCTCGATGCTCATATTGCGAATGCTGAACTCATTGCAAGTGCAGTTGGTGTTGTATCAGCAATAATAGACAATGTACCGCTAGTTGCCGCAACAATGGGAATGTATGATCTATCCTCCTTCCCTCGAGATTCCGAGTTCTGGCAACTGATTGCATTTTGTGCTGGTACTGGTGGATCCATGCTCATAATTGGATCTGCAGCTGGAGTTGCATTCATGGGGATGGAGAAGGTGGATTTCTTTTGGTACCTAAGAAAGGTCAGTGGTTTCGCATTTGCAGGGTATGCTGCTGGAATTGCTGCCTATTTAGCTCTTCATAACCTCAACATATCGTTACCAACAACTATTGCTGAGGTGCCCTTTATTTTGGGTTCATAA

Protein sequence

MASLSIGIHLPPSHHWKRFSLQSLSPISLPFHTRYASSSSRLRTPRLLRKNLLARAEDEAKGSSPASSSSFQQQPAPPGFSNQLEEFTSKSGTCDPLCSVDEISSQDFEASYQPKTDLLKAFAIFAAAITGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGLWVVRSIGAPSTEIAVSELTHSTAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKPRTLLWVIGGVTFFLSSILDNLTATIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTPIGDVTTTMLWIHGQISTVQTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVMASEQMAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKVPQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVVSAIIDNVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYLRKVSGFAFAGYAAGIAAYLALHNLNISLPTTIAEVPFILGS
Homology
BLAST of Moc10g04530 vs. NCBI nr
Match: XP_022149216.1 (sodium/proton antiporter 1 [Momordica charantia])

HSP 1 Score: 1097.8 bits (2838), Expect = 0.0e+00
Identity = 581/581 (100.00%), Postives = 581/581 (100.00%), Query Frame = 0

Query: 1   MASLSIGIHLPPSHHWKRFSLQSLSPISLPFHTRYASSSSRLRTPRLLRKNLLARAEDEA 60
           MASLSIGIHLPPSHHWKRFSLQSLSPISLPFHTRYASSSSRLRTPRLLRKNLLARAEDEA
Sbjct: 1   MASLSIGIHLPPSHHWKRFSLQSLSPISLPFHTRYASSSSRLRTPRLLRKNLLARAEDEA 60

Query: 61  KGSSPASSSSFQQQPAPPGFSNQLEEFTSKSGTCDPLCSVDEISSQDFEASYQPKTDLLK 120
           KGSSPASSSSFQQQPAPPGFSNQLEEFTSKSGTCDPLCSVDEISSQDFEASYQPKTDLLK
Sbjct: 61  KGSSPASSSSFQQQPAPPGFSNQLEEFTSKSGTCDPLCSVDEISSQDFEASYQPKTDLLK 120

Query: 121 AFAIFAAAITGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGL 180
           AFAIFAAAITGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGL
Sbjct: 121 AFAIFAAAITGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGL 180

Query: 181 WVVRSIGAPSTEIAVSELTHSTAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP 240
           WVVRSIGAPSTEIAVSELTHSTAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP
Sbjct: 181 WVVRSIGAPSTEIAVSELTHSTAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP 240

Query: 241 RTLLWVIGGVTFFLSSILDNLTATIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300
           RTLLWVIGGVTFFLSSILDNLTATIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP
Sbjct: 241 RTLLWVIGGVTFFLSSILDNLTATIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300

Query: 301 IGDVTTTMLWIHGQISTVQTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVMASEQ 360
           IGDVTTTMLWIHGQISTVQTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVMASEQ
Sbjct: 301 IGDVTTTMLWIHGQISTVQTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVMASEQ 360

Query: 361 MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV 420
           MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV
Sbjct: 361 MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV 420

Query: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVVSAIID 480
           PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVVSAIID
Sbjct: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVVSAIID 480

Query: 481 NVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540
           NVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL
Sbjct: 481 NVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540

Query: 541 RKVSGFAFAGYAAGIAAYLALHNLNISLPTTIAEVPFILGS 582
           RKVSGFAFAGYAAGIAAYLALHNLNISLPTTIAEVPFILGS
Sbjct: 541 RKVSGFAFAGYAAGIAAYLALHNLNISLPTTIAEVPFILGS 581

BLAST of Moc10g04530 vs. NCBI nr
Match: XP_038880556.1 (sodium/proton antiporter 1 [Benincasa hispida])

HSP 1 Score: 1016.9 bits (2628), Expect = 6.8e-293
Identity = 533/581 (91.74%), Postives = 553/581 (95.18%), Query Frame = 0

Query: 1   MASLSIGIHLPPSHHWKRFSLQSLSPISLPFHTRYASSSSRLRTPRLLRKNLLARAEDEA 60
           MASLSIGIHLPP HHW+RFSLQS SPISLPFHT Y SS SR+RTPRLL KNLL RAEDE 
Sbjct: 1   MASLSIGIHLPPPHHWRRFSLQSQSPISLPFHTSYGSSLSRVRTPRLLGKNLLVRAEDED 60

Query: 61  KGSSPASSSSFQQQPAPPGFSNQLEEFTSKSGTCDPLCSVDEISSQDFEASYQPKTDLLK 120
           K SSPA++SSF+QQ APP F+ QLEE +SKSGTCDPLCSVDEISSQDFE SYQPKTDL+K
Sbjct: 61  KSSSPATASSFEQQQAPPNFTKQLEELSSKSGTCDPLCSVDEISSQDFEDSYQPKTDLVK 120

Query: 121 AFAIFAAAITGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGL 180
           AFAIF AA+TGTVAINHSWVAANQDLAMVLLF IGYAGIIFEE+LAFNKSGVGLLMAVGL
Sbjct: 121 AFAIFVAALTGTVAINHSWVAANQDLAMVLLFGIGYAGIIFEEALAFNKSGVGLLMAVGL 180

Query: 181 WVVRSIGAPSTEIAVSELTHSTAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP 240
           WV+RSIGAPSTEIA SELTH+TAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP
Sbjct: 181 WVIRSIGAPSTEIAASELTHATAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP 240

Query: 241 RTLLWVIGGVTFFLSSILDNLTATIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300
           RTLLWVIG VTFFLSS+LDNLT TIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP
Sbjct: 241 RTLLWVIGFVTFFLSSVLDNLTTTIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300

Query: 301 IGDVTTTMLWIHGQISTVQTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVMASEQ 360
           IGDVTTTMLWIHGQISTV T+KDL++PSAVSLAVPLALLSLTSEVN KGQ+F NVMASEQ
Sbjct: 301 IGDVTTTMLWIHGQISTVPTLKDLIIPSAVSLAVPLALLSLTSEVNGKGQDFSNVMASEQ 360

Query: 361 MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV 420
           MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV
Sbjct: 361 MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV 420

Query: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVVSAIID 480
           PQALSRIDTQGALFFLGILLSVSSLEAAG+LRELANYLDAHI N ELIAS VGV+SAIID
Sbjct: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGVLRELANYLDAHIPNVELIASTVGVISAIID 480

Query: 481 NVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540
           NVPLVAATMGMYDLSSF +DSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL
Sbjct: 481 NVPLVAATMGMYDLSSFAQDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540

Query: 541 RKVSGFAFAGYAAGIAAYLALHNLNISLPTTIAEVPFILGS 582
           RKVSGFAFAGYAAGIAAYLALHN NISLPTTIAEVP ILGS
Sbjct: 541 RKVSGFAFAGYAAGIAAYLALHNFNISLPTTIAEVPSILGS 581

BLAST of Moc10g04530 vs. NCBI nr
Match: XP_004140712.1 (sodium/proton antiporter 1 [Cucumis sativus])

HSP 1 Score: 1008.4 bits (2606), Expect = 2.4e-290
Identity = 533/581 (91.74%), Postives = 550/581 (94.66%), Query Frame = 0

Query: 1   MASLSIGIHLPPSHHWKRFSLQSLSPISLPFHTRYASSSSRLRTPRLLRKNLLARAEDEA 60
           MASLSIGIHLPPSHHWKRFSLQS SPISLPFHT Y SS +R+RTPRLL KNLL RAEDE 
Sbjct: 1   MASLSIGIHLPPSHHWKRFSLQSQSPISLPFHTSYGSSLNRVRTPRLLGKNLLVRAEDED 60

Query: 61  KGSSPASSSSFQQQPAPPGFSNQLEEFTSKSGTCDPLCSVDEISSQDFEASYQPKTDLLK 120
           K     SSSSF+QQ APP FS QLEE +SK GTCDPLCSVDE+SSQDFE SYQPKTDL+K
Sbjct: 61  K-----SSSSFEQQQAPPNFSKQLEELSSKPGTCDPLCSVDEVSSQDFEDSYQPKTDLVK 120

Query: 121 AFAIFAAAITGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGL 180
           AFAIFAAA+TGTVAINHSWVAANQDLAMVLLF IGYAGIIFEESLAFNKSGVGLLMAVGL
Sbjct: 121 AFAIFAAALTGTVAINHSWVAANQDLAMVLLFGIGYAGIIFEESLAFNKSGVGLLMAVGL 180

Query: 181 WVVRSIGAPSTEIAVSELTHSTAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP 240
           WV+RSIGAPSTEIA SELTH+TAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTD ITTRKP
Sbjct: 181 WVIRSIGAPSTEIAASELTHATAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDGITTRKP 240

Query: 241 RTLLWVIGGVTFFLSSILDNLTATIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300
           RTLLWVIG VTFFLSSILDNLT TIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP
Sbjct: 241 RTLLWVIGFVTFFLSSILDNLTTTIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300

Query: 301 IGDVTTTMLWIHGQISTVQTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVMASEQ 360
           IGDVTTTMLWIHGQIST+ T+KDLV+PSAVSLAVPLALLSLTSEVN KGQ+F NVMASEQ
Sbjct: 301 IGDVTTTMLWIHGQISTMPTLKDLVIPSAVSLAVPLALLSLTSEVNGKGQDFSNVMASEQ 360

Query: 361 MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV 420
           MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQ+LKV
Sbjct: 361 MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQRLKV 420

Query: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVVSAIID 480
           PQALSRIDTQGALFFLGILLSVSSLEAAG+LRELANYLDAHI N ELIASAVGV+SAIID
Sbjct: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGVLRELANYLDAHIPNVELIASAVGVISAIID 480

Query: 481 NVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540
           NVPLVAATMGMYDLSSF +DSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL
Sbjct: 481 NVPLVAATMGMYDLSSFAQDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540

Query: 541 RKVSGFAFAGYAAGIAAYLALHNLNISLPTTIAEVPFILGS 582
           RKVSGFAFAGYAAGIAAYLALHN NISLPTTIAEVP ILGS
Sbjct: 541 RKVSGFAFAGYAAGIAAYLALHNFNISLPTTIAEVPSILGS 576

BLAST of Moc10g04530 vs. NCBI nr
Match: XP_008456161.1 (PREDICTED: Na(+)/H(+) antiporter NhaD [Cucumis melo])

HSP 1 Score: 1007.3 bits (2603), Expect = 5.4e-290
Identity = 532/581 (91.57%), Postives = 552/581 (95.01%), Query Frame = 0

Query: 1   MASLSIGIHLPPSHHWKRFSLQSLSPISLPFHTRYASSSSRLRTPRLLRKNLLARAEDEA 60
           MASLSIGIHLPPSHHWKRF+LQS SPISLPFHT Y SS SR+RTPRLL KNLL RAEDE 
Sbjct: 1   MASLSIGIHLPPSHHWKRFALQSQSPISLPFHTSYGSSLSRVRTPRLLGKNLLVRAEDEN 60

Query: 61  KGSSPASSSSFQQQPAPPGFSNQLEEFTSKSGTCDPLCSVDEISSQDFEASYQPKTDLLK 120
           K     SSSSF+Q  APP FS QLEE +SKSGTCDPLCSVDE+SSQDFE SYQPKTDL+K
Sbjct: 61  K-----SSSSFEQLQAPPSFSKQLEELSSKSGTCDPLCSVDEVSSQDFEDSYQPKTDLVK 120

Query: 121 AFAIFAAAITGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGL 180
           AFAIFAAA+TGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGL
Sbjct: 121 AFAIFAAALTGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGL 180

Query: 181 WVVRSIGAPSTEIAVSELTHSTAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP 240
           WV+RSIGAPSTEIA SELTH+TAEVSEIVFFLLGAMTIVEI+DAHQGFKLVTDSITTRKP
Sbjct: 181 WVIRSIGAPSTEIAASELTHATAEVSEIVFFLLGAMTIVEIIDAHQGFKLVTDSITTRKP 240

Query: 241 RTLLWVIGGVTFFLSSILDNLTATIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300
           RTLLWVIG VTFFLSSILDNLT TIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP
Sbjct: 241 RTLLWVIGFVTFFLSSILDNLTTTIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300

Query: 301 IGDVTTTMLWIHGQISTVQTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVMASEQ 360
           IGDVTTTMLWIHGQI+T+ T+KDLV+PSAVSLAVPLALLSLTSEVN KGQ+F +VMASEQ
Sbjct: 301 IGDVTTTMLWIHGQITTMPTLKDLVIPSAVSLAVPLALLSLTSEVNGKGQDFSDVMASEQ 360

Query: 361 MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV 420
           MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQ+LKV
Sbjct: 361 MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQRLKV 420

Query: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVVSAIID 480
           PQALSRIDTQGALFFLGILLSVSSLEAAG+LRELANYLDAHI N ELIASAVGV+SAIID
Sbjct: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGVLRELANYLDAHIPNVELIASAVGVISAIID 480

Query: 481 NVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540
           NVPLVAATMGMYDLSSF +DSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL
Sbjct: 481 NVPLVAATMGMYDLSSFAQDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540

Query: 541 RKVSGFAFAGYAAGIAAYLALHNLNISLPTTIAEVPFILGS 582
           RKVSGFAFAGYAAGIAAYLALHN NISLPTTIAEVP ILGS
Sbjct: 541 RKVSGFAFAGYAAGIAAYLALHNFNISLPTTIAEVPSILGS 576

BLAST of Moc10g04530 vs. NCBI nr
Match: KAG6576940.1 (Sodium/proton antiporter 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 988.8 bits (2555), Expect = 2.0e-284
Identity = 526/580 (90.69%), Postives = 543/580 (93.62%), Query Frame = 0

Query: 1   MASLSIGIHLPPSHHWKRFSLQSLSPISLPFHTRYASSSSRLRTPRLLRKNLLARAEDEA 60
           MASLSIGIHLPPS HWKRFS  S SP+SLPFHT Y SS SR+R PRLL KNLLARAEDE 
Sbjct: 1   MASLSIGIHLPPSRHWKRFSFHSHSPVSLPFHTSYGSSLSRIRAPRLLGKNLLARAEDEN 60

Query: 61  KGSSPASSSSFQQQPAPPGFSNQLEEFTSKSGTCDPLCSVDEISSQDFEASYQPKTDLLK 120
           K     SSSSFQQQ APP FS QLEE +SKSGTCDPLCSVDEISS+DFE SYQP+ DL+K
Sbjct: 61  K-----SSSSFQQQQAPPNFSKQLEELSSKSGTCDPLCSVDEISSRDFEDSYQPQADLVK 120

Query: 121 AFAIFAAAITGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGL 180
           AFAI AAA+TG VAINHSWVAANQDLAMVLLFSIGYAGIIFEE+LAFNKSGVGLLMAVGL
Sbjct: 121 AFAILAAALTGIVAINHSWVAANQDLAMVLLFSIGYAGIIFEEALAFNKSGVGLLMAVGL 180

Query: 181 WVVRSIGAPSTEIAVSELTHSTAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP 240
           WVVRSIGAPSTEIA SELTH+TAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP
Sbjct: 181 WVVRSIGAPSTEIAASELTHATAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP 240

Query: 241 RTLLWVIGGVTFFLSSILDNLTATIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300
           RTLLWVIG VTFFLSSILDNLT TIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP
Sbjct: 241 RTLLWVIGIVTFFLSSILDNLTTTIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300

Query: 301 IGDVTTTMLWIHGQISTVQTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVMASEQ 360
           IGDVTTTMLWIHGQIST+ T+KDL +PSAVSL VPL+LLSLTSEVN KGQ+F NVMASEQ
Sbjct: 301 IGDVTTTMLWIHGQISTMPTLKDLFIPSAVSLIVPLSLLSLTSEVNGKGQDFSNVMASEQ 360

Query: 361 MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV 420
           MAPRGKLVFSVGVGALV VPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV
Sbjct: 361 MAPRGKLVFSVGVGALVSVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV 420

Query: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVVSAIID 480
           PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHI N ELIASAVGV+SAIID
Sbjct: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIPNTELIASAVGVISAIID 480

Query: 481 NVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540
           NVPLVAATMGMYDLSSF +DSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL
Sbjct: 481 NVPLVAATMGMYDLSSFAQDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540

Query: 541 RKVSGFAFAGYAAGIAAYLALHNLNISLPTTIAEVPFILG 581
           RKVSGFAFAGYAAGIAAYLALHNLN SLPTTIAEVP I+G
Sbjct: 541 RKVSGFAFAGYAAGIAAYLALHNLNTSLPTTIAEVPSIMG 575

BLAST of Moc10g04530 vs. ExPASy Swiss-Prot
Match: Q9LT68 (Sodium/proton antiporter 1 OS=Arabidopsis thaliana OX=3702 GN=NHD1 PE=2 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 1.0e-243
Identity = 452/586 (77.13%), Postives = 503/586 (85.84%), Query Frame = 0

Query: 1   MASLSIGIHLPPSHH-WKRFSLQSLSPIS----LPFHTRYASSSSRLRTPRLLRKNLLAR 60
           MA   IG H  P H   KR  + + SPIS    LP +  ++  S    + RL +  +L R
Sbjct: 1   MAVFPIGSHFAPPHQLTKRHVIATSSPISISTRLPQNVSFSKVSGVTGSTRLSKHGVLVR 60

Query: 61  AEDEAKGSSPASSSSFQQQPAPPGFSNQLEEFTSKSGTCDPLCSVDEISSQDFEASYQPK 120
           AED+ + SS  SS     +P         E+    SG CDPLCSVDE SS  FEA+YQPK
Sbjct: 61  AEDKIRSSSSPSS---LDEPID-------EDLMDSSGLCDPLCSVDEPSSSYFEANYQPK 120

Query: 121 TDLLKAFAIFAAAITGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLL 180
           TD++KA AI AAA+TGT AINHSWVAANQD+AM LLF IGYAGIIFEESLAFNKSG+GLL
Sbjct: 121 TDIIKALAILAAALTGTAAINHSWVAANQDVAMALLFGIGYAGIIFEESLAFNKSGIGLL 180

Query: 181 MAVGLWVVRSIGAPSTEIAVSELTHSTAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSI 240
           MAV LWVVRSIGAPSTEIAV +L H+TAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTD+I
Sbjct: 181 MAVSLWVVRSIGAPSTEIAVLDLQHATAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDNI 240

Query: 241 TTRKPRTLLWVIGGVTFFLSSILDNLTATIIMVSLLRKLVPPSEYRKLLGAVIVIAANAG 300
           TTRKP+TLLWV+G VTFFLSSILDNLT+TI+MVSL+RKLVP SEYRKLLG V+VIAANAG
Sbjct: 241 TTRKPKTLLWVVGFVTFFLSSILDNLTSTIVMVSLIRKLVPQSEYRKLLGGVVVIAANAG 300

Query: 301 GAWTPIGDVTTTMLWIHGQISTVQTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNV 360
           GAWTPIGDVTTTMLWIHGQIST+ T+KDL +PS VSLAVPLAL+SLTSEVN K Q+  +V
Sbjct: 301 GAWTPIGDVTTTMLWIHGQISTLPTMKDLFLPSVVSLAVPLALMSLTSEVNGKEQDPKDV 360

Query: 361 MASEQMAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESER 420
           +ASE+MAPRGKLVF VG+GALVFVPVFKALTGLPPYMG+LLGLG+LWILTDAIHYGESER
Sbjct: 361 LASEKMAPRGKLVFGVGLGALVFVPVFKALTGLPPYMGILLGLGVLWILTDAIHYGESER 420

Query: 421 QKLKVPQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVV 480
           QKLKVPQALSRIDTQGALFFLGILLSVSSLEAAGILRE+ANYLDA+I N ELIASA+GVV
Sbjct: 421 QKLKVPQALSRIDTQGALFFLGILLSVSSLEAAGILREIANYLDANIPNVELIASAIGVV 480

Query: 481 SAIIDNVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVD 540
           SAIIDNVPLVAATMGMYDL+SFP+DSEFWQLIAFCAGTGGSML+IGSAAGVAFMGMEKVD
Sbjct: 481 SAIIDNVPLVAATMGMYDLTSFPQDSEFWQLIAFCAGTGGSMLVIGSAAGVAFMGMEKVD 540

Query: 541 FFWYLRKVSGFAFAGYAAGIAAYLALHNLNISLPTTIAEVPFILGS 582
           FFWY RKVSGFAFAGYAAGIAAYLA+HNL+  +PTT+A++PF+ GS
Sbjct: 541 FFWYFRKVSGFAFAGYAAGIAAYLAVHNLHFEIPTTVAQIPFLTGS 576

BLAST of Moc10g04530 vs. ExPASy Swiss-Prot
Match: Q9C6D3 (Sodium/proton antiporter 2 OS=Arabidopsis thaliana OX=3702 GN=NHD2 PE=3 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 2.2e-177
Identity = 322/418 (77.03%), Postives = 369/418 (88.28%), Query Frame = 0

Query: 148 MVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGLWVVRSIGAPSTEIAVSELTHSTAEVSE 207
           M LLF IGY  IIFEESL+F+KSG+ LLMAV LWVVRSI   S EI   EL H+T+EVS+
Sbjct: 1   MALLFGIGYVLIIFEESLSFSKSGIALLMAVSLWVVRSI-ETSVEIVTLELQHATSEVSQ 60

Query: 208 IVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKPRTLLWVIGGVTFFLSSILDNLTATIIM 267
           IVF++LGAMTIVEI+DAHQGFKLVTD IT+RKP+ LLWVIG  TFFLSS+LDNLT+TI+M
Sbjct: 61  IVFYMLGAMTIVEIIDAHQGFKLVTDCITSRKPKILLWVIGFATFFLSSVLDNLTSTIVM 120

Query: 268 VSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTPIGDVTTTMLWIHGQISTVQTVKDLVVP 327
           VSLLR+L+PPSEYRKLLGAV+VIAANAGGAWTPIGDVTTTMLWIHG IST  T+K+L +P
Sbjct: 121 VSLLRRLIPPSEYRKLLGAVVVIAANAGGAWTPIGDVTTTMLWIHGHISTFSTIKNLFLP 180

Query: 328 SAVSLAVPLALLSLTSEVNVKGQEFP--NVMASEQMAPRGKLVFSVGVGALVFVPVFKAL 387
           SA+SL VPLAL+SLTSEV+  G   P   ++A ++ APRGKLVF VG GAL+FVP+FK+L
Sbjct: 181 SAISLVVPLALMSLTSEVHGMGLNTPPTPLLAYDRSAPRGKLVFGVGFGALLFVPLFKSL 240

Query: 388 TGLPPYMGMLLGLGILWILTDAIHYGESERQKLKVPQALSRIDTQGALFFLGILLSVSSL 447
           TGLPPYMG+LLGLG++WILTD IHYG+ ERQ LK+P ALSRID+QGALFFLGILLS+SSL
Sbjct: 241 TGLPPYMGILLGLGVIWILTDVIHYGDLERQHLKLPHALSRIDSQGALFFLGILLSMSSL 300

Query: 448 EAAGILRELANYLDAHIANAELIASAVGVVSAIIDNVPLVAATMGMYDLSSFPRDSEFWQ 507
           +AAGIL+ +ANYLDAHIAN ELIAS +GVVSAIIDNVPLVAATMGMYDLS+FP+DSEFWQ
Sbjct: 301 DAAGILKVIANYLDAHIANVELIASIIGVVSAIIDNVPLVAATMGMYDLSTFPQDSEFWQ 360

Query: 508 LIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYLRKVSGFAFAGYAAGIAAYLALHN 564
           LI+FCAGTGGSMLI GSAAGV FM MEKV+FFWY RKVSGFAFAG+ AGI  YLA+HN
Sbjct: 361 LISFCAGTGGSMLITGSAAGVIFMSMEKVNFFWYFRKVSGFAFAGFTAGIMTYLAVHN 417

BLAST of Moc10g04530 vs. ExPASy Swiss-Prot
Match: A5F120 (Na(+)/H(+) antiporter NhaD OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) OX=345073 GN=nhaD PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 5.0e-36
Identity = 138/450 (30.67%), Postives = 220/450 (48.89%), Query Frame = 0

Query: 147 AMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGLWVVRSIG-----APSTEIAVSELTHS 206
           A +L+F+I Y  ++ EE L   KS   LL A  +W +  IG       STE+A   L H+
Sbjct: 37  AALLIFAIAYTLVMLEEYLQLRKSKPVLLAAGLIWAM--IGYVYQQTGSTEVARQALEHN 96

Query: 207 TAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTR--KPRTLLWVIGGVTFFLSSILD 266
             E +E++ FLL AMT +  ++  + F  +   +  R     TL W+ G + FF+S I D
Sbjct: 97  LLEYAELLLFLLVAMTYISAMEERRLFDALKAWMINRGFNFHTLFWITGWLAFFISPIAD 156

Query: 267 NLTATIIMVSLLRKLVPPS-EYRKLLGAVIVIAANAGGAWTPIGDVTTTMLWIHGQISTV 326
           NLT  ++M +++ K+   + ++  L    IVIAANAGGA++P GD+TT M+W  G +S +
Sbjct: 157 NLTTALLMCAVVMKVGGENPKFVSLACINIVIAANAGGAFSPFGDITTLMVWQAGHVSFL 216

Query: 327 QTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVMASEQMAPRGKLVFSVGVGALVF 386
           + + DL +PS  +  VP  ++SL     V  Q   ++    ++    K +  + +  ++ 
Sbjct: 217 EFM-DLFLPSLANYLVPALVMSLF----VPHQTPSSIQEVVELKRGAKRIVVLFLFTILS 276

Query: 387 VPVFKALTGLPPYMGMLLGLGILWIL--------------TDAIHYGESERQKLK----- 446
              F A    PP +GM++GL  L                   AI   +++   LK     
Sbjct: 277 AIGFHAFFHFPPVIGMMMGLAYLQFFGYFLRKTLARSLAKKTAIAMAKNDEAALKRIGSV 336

Query: 447 ----VPQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVV 506
               V +++S  +    LFF G+++ V  L   G L  ++  L     N       VG++
Sbjct: 337 VPFDVFRSISHAEWDTLLFFYGVVMCVGGLSLLGYLGLVSEILYTE-WNPIWANVLVGLL 396

Query: 507 SAIIDNVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGME--K 564
           S+++DN+P++ A + M    S       W L+   AG GGS+L IGSAAGVA MG    K
Sbjct: 397 SSVVDNIPVMFAVLSMQPEMSLGN----WLLVTLTAGVGGSLLSIGSAAGVALMGAAHGK 456

BLAST of Moc10g04530 vs. ExPASy Swiss-Prot
Match: Q56EB3 (Na(+)/H(+) antiporter NhaD OS=Alkalimonas amylolytica OX=152573 GN=nhaD PE=1 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 1.1e-35
Identity = 135/448 (30.13%), Postives = 223/448 (49.78%), Query Frame = 0

Query: 149 VLLFSIGYAGIIFEESLAFNKSGVGLLMAVGLWVVRS---IGAPSTEIAVSELTHSTAEV 208
           + +F + Y  ++ EE L   KS   L+ A  +W++     I     ++  +   H+  E 
Sbjct: 42  IAIFVVAYVLVMGEEKLHMRKSKPVLVAAGLIWILIGWVYISRDIPDVTEAAFRHNLLEF 101

Query: 209 SEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTR--KPRTLLWVIGGVTFFLSSILDNLTA 268
           +E++ FLL AMT +  ++  + F  +   +  +    + L W+ G ++FF+S I DNLT 
Sbjct: 102 AELMLFLLVAMTYINALEERRLFDALRAWMIRKGFSYQNLFWITGFLSFFISPIADNLTT 161

Query: 269 TIIMVSLLRKLVP-PSEYRKLLGAVIVIAANAGGAWTPIGDVTTTMLWIHGQISTVQTVK 328
            ++M +++ K+      +  L    IVIAANAGGA++P GD+TT M+W  G +  +    
Sbjct: 162 ALLMCAVVMKVAEGDKRFINLCCVNIVIAANAGGAFSPFGDITTLMVWQAGLV-RIDEFL 221

Query: 329 DLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVMASEQMAPRGKL-VFSVGVGALVFVPV 388
            L  P+ V+  +P A++S   E     +  P+ +  +    RG L + ++ +  +    +
Sbjct: 222 VLFFPALVNYLIPAAVMSFFVE-----KRQPSAVYEDVELKRGALRILTLFLLTVATAVL 281

Query: 389 FKALTGLPPYMGMLLGLGILWIL--------------TDAIHYGESERQKLK-------- 448
             +L  LPP +GM++GLG L                   A+   E +++KLK        
Sbjct: 282 CHSLLHLPPVLGMMMGLGYLQFFGYFLRMTLPGSLARKRAMAEREGDQEKLKRLGGVVPF 341

Query: 449 -VPQALSRIDTQGALFFLGILLSVSSLEAAGILRELAN--YLDAHIANAELIASAVGVVS 508
            V   +SR +    LFF GI++ V  L   G L  +++  Y   +  +A ++   +GV+S
Sbjct: 342 DVFSRVSRAEWDTLLFFYGIVMCVGGLGFLGYLGLMSDLLYEGWNPTSANIL---LGVIS 401

Query: 509 AIIDNVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEK--V 563
           A+IDN+P++ A + M    S       W LI   AG GGS+L IGSAAGVA MG  +   
Sbjct: 402 AVIDNIPVMFAVLAMQPEMSHGH----WLLITLTAGVGGSLLSIGSAAGVALMGQARGYY 461

BLAST of Moc10g04530 vs. ExPASy Swiss-Prot
Match: E1VBT7 (Na(+)/H(+) antiporter NhaD OS=Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9) OX=768066 GN=nhaD PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 4.8e-31
Identity = 135/452 (29.87%), Postives = 218/452 (48.23%), Query Frame = 0

Query: 149 VLLFSIGYAGIIFEESLAFNKS-----GVGLLMAVGLWVVRSIGAP-STEIAVSELTHST 208
           V LF   Y  ++ EE L   KS       GL+ A+  WV    G P ++E A SE   + 
Sbjct: 54  VTLFLAAYVLVMAEEKLHMRKSKPVLVAAGLIWAMIGWVYVHAGLPDASEEAFSE---TL 113

Query: 209 AEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTR--KPRTLLWVIGGVTFFLSSILDN 268
            E SE++ FLL AMT +  ++  + F  +   +  +    R+L W+ G + F++S+I +N
Sbjct: 114 LEYSELLLFLLVAMTYINAMEERRVFDKLRAWLVEKGFSYRSLFWITGILAFWISTIANN 173

Query: 269 LTATIIMVSLLRKLVP-PSEYRKLLGAVIVIAANAGGAWTPIGDVTTTMLWIHGQISTVQ 328
           LT  ++M +++ K+      +  L    +V+A+NAGGA++P GD+TT M+W   ++   Q
Sbjct: 174 LTTAMLMCAVVLKVAEGDKRFINLCCINVVVASNAGGAFSPFGDITTLMVW-QAKLVEFQ 233

Query: 329 TVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVMASEQMAPRGKLVFSVGVGALVFV 388
              +L+ P+ V+  VP  ++SL  + N K       +  ++ A R  L+F V    +V  
Sbjct: 234 EFFELLGPALVNYLVPAIVMSLFIK-NRKPAALEEHIWLKRGARRIVLLFLV---TIVIS 293

Query: 389 PVFKALTGLPPYMGMLLGLGILWILTDAIHYG---ESERQKLKVPQ-------------- 448
            +   +  LPP +GM+ GLG L      +        ER++ +  Q              
Sbjct: 294 VLCHTMLNLPPALGMMTGLGFLQFFGYYLRQSLPRSLERKRTRYSQRGDWRKLESLGSVV 353

Query: 449 ------ALSRIDTQGALFFLGILLSVSSLEAAGILRELANYL----DAHIANAELIASAV 508
                  ++R +    LFF G+++SV  L   G L  L+  L    D   AN  L     
Sbjct: 354 PFDVFTRIARSEWDTLLFFYGVVMSVGGLGFMGYLALLSETLYTGWDPVWANIVL----- 413

Query: 509 GVVSAIIDNVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGME 563
           G+VS+++DN+P++ A + M    S       W LI   AG GGS+L +GSAAGVA MG  
Sbjct: 414 GLVSSVVDNIPVMFAVISMEPDMSMGN----WLLITLTAGVGGSLLSVGSAAGVALMGQA 473

BLAST of Moc10g04530 vs. ExPASy TrEMBL
Match: A0A6J1D7Q2 (sodium/proton antiporter 1 OS=Momordica charantia OX=3673 GN=LOC111017691 PE=4 SV=1)

HSP 1 Score: 1097.8 bits (2838), Expect = 0.0e+00
Identity = 581/581 (100.00%), Postives = 581/581 (100.00%), Query Frame = 0

Query: 1   MASLSIGIHLPPSHHWKRFSLQSLSPISLPFHTRYASSSSRLRTPRLLRKNLLARAEDEA 60
           MASLSIGIHLPPSHHWKRFSLQSLSPISLPFHTRYASSSSRLRTPRLLRKNLLARAEDEA
Sbjct: 1   MASLSIGIHLPPSHHWKRFSLQSLSPISLPFHTRYASSSSRLRTPRLLRKNLLARAEDEA 60

Query: 61  KGSSPASSSSFQQQPAPPGFSNQLEEFTSKSGTCDPLCSVDEISSQDFEASYQPKTDLLK 120
           KGSSPASSSSFQQQPAPPGFSNQLEEFTSKSGTCDPLCSVDEISSQDFEASYQPKTDLLK
Sbjct: 61  KGSSPASSSSFQQQPAPPGFSNQLEEFTSKSGTCDPLCSVDEISSQDFEASYQPKTDLLK 120

Query: 121 AFAIFAAAITGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGL 180
           AFAIFAAAITGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGL
Sbjct: 121 AFAIFAAAITGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGL 180

Query: 181 WVVRSIGAPSTEIAVSELTHSTAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP 240
           WVVRSIGAPSTEIAVSELTHSTAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP
Sbjct: 181 WVVRSIGAPSTEIAVSELTHSTAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP 240

Query: 241 RTLLWVIGGVTFFLSSILDNLTATIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300
           RTLLWVIGGVTFFLSSILDNLTATIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP
Sbjct: 241 RTLLWVIGGVTFFLSSILDNLTATIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300

Query: 301 IGDVTTTMLWIHGQISTVQTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVMASEQ 360
           IGDVTTTMLWIHGQISTVQTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVMASEQ
Sbjct: 301 IGDVTTTMLWIHGQISTVQTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVMASEQ 360

Query: 361 MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV 420
           MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV
Sbjct: 361 MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV 420

Query: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVVSAIID 480
           PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVVSAIID
Sbjct: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVVSAIID 480

Query: 481 NVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540
           NVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL
Sbjct: 481 NVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540

Query: 541 RKVSGFAFAGYAAGIAAYLALHNLNISLPTTIAEVPFILGS 582
           RKVSGFAFAGYAAGIAAYLALHNLNISLPTTIAEVPFILGS
Sbjct: 541 RKVSGFAFAGYAAGIAAYLALHNLNISLPTTIAEVPFILGS 581

BLAST of Moc10g04530 vs. ExPASy TrEMBL
Match: A0A1S3C2P8 (Na(+)/H(+) antiporter NhaD OS=Cucumis melo OX=3656 GN=LOC103496182 PE=4 SV=1)

HSP 1 Score: 1007.3 bits (2603), Expect = 2.6e-290
Identity = 532/581 (91.57%), Postives = 552/581 (95.01%), Query Frame = 0

Query: 1   MASLSIGIHLPPSHHWKRFSLQSLSPISLPFHTRYASSSSRLRTPRLLRKNLLARAEDEA 60
           MASLSIGIHLPPSHHWKRF+LQS SPISLPFHT Y SS SR+RTPRLL KNLL RAEDE 
Sbjct: 1   MASLSIGIHLPPSHHWKRFALQSQSPISLPFHTSYGSSLSRVRTPRLLGKNLLVRAEDEN 60

Query: 61  KGSSPASSSSFQQQPAPPGFSNQLEEFTSKSGTCDPLCSVDEISSQDFEASYQPKTDLLK 120
           K     SSSSF+Q  APP FS QLEE +SKSGTCDPLCSVDE+SSQDFE SYQPKTDL+K
Sbjct: 61  K-----SSSSFEQLQAPPSFSKQLEELSSKSGTCDPLCSVDEVSSQDFEDSYQPKTDLVK 120

Query: 121 AFAIFAAAITGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGL 180
           AFAIFAAA+TGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGL
Sbjct: 121 AFAIFAAALTGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGL 180

Query: 181 WVVRSIGAPSTEIAVSELTHSTAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP 240
           WV+RSIGAPSTEIA SELTH+TAEVSEIVFFLLGAMTIVEI+DAHQGFKLVTDSITTRKP
Sbjct: 181 WVIRSIGAPSTEIAASELTHATAEVSEIVFFLLGAMTIVEIIDAHQGFKLVTDSITTRKP 240

Query: 241 RTLLWVIGGVTFFLSSILDNLTATIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300
           RTLLWVIG VTFFLSSILDNLT TIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP
Sbjct: 241 RTLLWVIGFVTFFLSSILDNLTTTIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300

Query: 301 IGDVTTTMLWIHGQISTVQTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVMASEQ 360
           IGDVTTTMLWIHGQI+T+ T+KDLV+PSAVSLAVPLALLSLTSEVN KGQ+F +VMASEQ
Sbjct: 301 IGDVTTTMLWIHGQITTMPTLKDLVIPSAVSLAVPLALLSLTSEVNGKGQDFSDVMASEQ 360

Query: 361 MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV 420
           MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQ+LKV
Sbjct: 361 MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQRLKV 420

Query: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVVSAIID 480
           PQALSRIDTQGALFFLGILLSVSSLEAAG+LRELANYLDAHI N ELIASAVGV+SAIID
Sbjct: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGVLRELANYLDAHIPNVELIASAVGVISAIID 480

Query: 481 NVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540
           NVPLVAATMGMYDLSSF +DSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL
Sbjct: 481 NVPLVAATMGMYDLSSFAQDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540

Query: 541 RKVSGFAFAGYAAGIAAYLALHNLNISLPTTIAEVPFILGS 582
           RKVSGFAFAGYAAGIAAYLALHN NISLPTTIAEVP ILGS
Sbjct: 541 RKVSGFAFAGYAAGIAAYLALHNFNISLPTTIAEVPSILGS 576

BLAST of Moc10g04530 vs. ExPASy TrEMBL
Match: A0A6J1JA42 (sodium/proton antiporter 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482646 PE=4 SV=1)

HSP 1 Score: 988.0 bits (2553), Expect = 1.6e-284
Identity = 526/580 (90.69%), Postives = 543/580 (93.62%), Query Frame = 0

Query: 1   MASLSIGIHLPPSHHWKRFSLQSLSPISLPFHTRYASSSSRLRTPRLLRKNLLARAEDEA 60
           MASLSIGIHLPPS HWKRFS  S SP+SLPFHT Y SS SR R PRLL KNLLARAEDE 
Sbjct: 1   MASLSIGIHLPPSQHWKRFSFHSHSPVSLPFHTSYGSSLSRTRAPRLLGKNLLARAEDED 60

Query: 61  KGSSPASSSSFQQQPAPPGFSNQLEEFTSKSGTCDPLCSVDEISSQDFEASYQPKTDLLK 120
           K     SSSSFQQQ APP FS QLEE +SKSGTCDPLCSVDEISS+DFE SYQP+ DL+K
Sbjct: 61  K-----SSSSFQQQQAPPNFSKQLEELSSKSGTCDPLCSVDEISSRDFEDSYQPQADLVK 120

Query: 121 AFAIFAAAITGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGL 180
           AFAI AAA+TG VAINHSWVAANQDLAMVLLFSIGYAGIIFEE+LAFNKSGVGLLMAVGL
Sbjct: 121 AFAILAAALTGIVAINHSWVAANQDLAMVLLFSIGYAGIIFEEALAFNKSGVGLLMAVGL 180

Query: 181 WVVRSIGAPSTEIAVSELTHSTAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP 240
           WVVRSIGAPSTEIA SELTH+TAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP
Sbjct: 181 WVVRSIGAPSTEIAASELTHATAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP 240

Query: 241 RTLLWVIGGVTFFLSSILDNLTATIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300
           RTLLWVIG VTFFLSSILDNLT TIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP
Sbjct: 241 RTLLWVIGIVTFFLSSILDNLTTTIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300

Query: 301 IGDVTTTMLWIHGQISTVQTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVMASEQ 360
           IGDVTTTMLWIHGQIST+ T+KDL +PSAVSL VPL+LLSLTSEVN KGQ+F NVMASEQ
Sbjct: 301 IGDVTTTMLWIHGQISTMPTLKDLFIPSAVSLIVPLSLLSLTSEVNGKGQDFSNVMASEQ 360

Query: 361 MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV 420
           MAPRGKLVFSVGVGALV VPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV
Sbjct: 361 MAPRGKLVFSVGVGALVSVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV 420

Query: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVVSAIID 480
           PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHI NAELIASAVGV+SAIID
Sbjct: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIPNAELIASAVGVISAIID 480

Query: 481 NVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540
           NVPLVAATMGMYDLSSF +DSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL
Sbjct: 481 NVPLVAATMGMYDLSSFAQDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540

Query: 541 RKVSGFAFAGYAAGIAAYLALHNLNISLPTTIAEVPFILG 581
           RKVSGFAFAGYAAGIAAYLALHNLN SLPTTIAE+P I+G
Sbjct: 541 RKVSGFAFAGYAAGIAAYLALHNLNTSLPTTIAEMPTIMG 575

BLAST of Moc10g04530 vs. ExPASy TrEMBL
Match: A0A6J1E3Q7 (sodium/proton antiporter 1-like OS=Cucurbita moschata OX=3662 GN=LOC111430554 PE=4 SV=1)

HSP 1 Score: 987.6 bits (2552), Expect = 2.1e-284
Identity = 525/580 (90.52%), Postives = 543/580 (93.62%), Query Frame = 0

Query: 1   MASLSIGIHLPPSHHWKRFSLQSLSPISLPFHTRYASSSSRLRTPRLLRKNLLARAEDEA 60
           MA+LSIGIHLPPS HWKRFS  S SP+SLPFHT Y SS SR+R PRLL KNLLARAEDE 
Sbjct: 1   MATLSIGIHLPPSRHWKRFSFHSHSPVSLPFHTSYGSSLSRIRAPRLLGKNLLARAEDED 60

Query: 61  KGSSPASSSSFQQQPAPPGFSNQLEEFTSKSGTCDPLCSVDEISSQDFEASYQPKTDLLK 120
           K     SSSSFQQQ APP FS QLEE +SKSGTCDPLCSVDEISS+DFE SYQP+ DL+K
Sbjct: 61  K-----SSSSFQQQQAPPNFSKQLEELSSKSGTCDPLCSVDEISSRDFEDSYQPQADLVK 120

Query: 121 AFAIFAAAITGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGL 180
           AFAI AAA+TG VAINHSWVAANQDLAMVLLFSIGYAGIIFEE+LAFNKSGVGLLMAVGL
Sbjct: 121 AFAILAAALTGIVAINHSWVAANQDLAMVLLFSIGYAGIIFEEALAFNKSGVGLLMAVGL 180

Query: 181 WVVRSIGAPSTEIAVSELTHSTAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP 240
           WVVRSIGAPSTEIA SELTH+TAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP
Sbjct: 181 WVVRSIGAPSTEIAASELTHATAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKP 240

Query: 241 RTLLWVIGGVTFFLSSILDNLTATIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300
           RTLLWVIG VTFFLSSILDNLT TIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP
Sbjct: 241 RTLLWVIGIVTFFLSSILDNLTTTIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTP 300

Query: 301 IGDVTTTMLWIHGQISTVQTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVMASEQ 360
           IGDVTTTMLWIHGQIST+ T+KDL +PSAVSL VPL+LLSLTSEVN KGQ+F NVMASEQ
Sbjct: 301 IGDVTTTMLWIHGQISTMPTLKDLFIPSAVSLIVPLSLLSLTSEVNGKGQDFSNVMASEQ 360

Query: 361 MAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV 420
           MAPRGKLVFSVGVGALV VPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV
Sbjct: 361 MAPRGKLVFSVGVGALVSVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQKLKV 420

Query: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVVSAIID 480
           PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHI N ELIASAVGV+SAIID
Sbjct: 421 PQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIPNTELIASAVGVISAIID 480

Query: 481 NVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540
           NVPLVAATMGMYDLSSF +DSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL
Sbjct: 481 NVPLVAATMGMYDLSSFAQDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYL 540

Query: 541 RKVSGFAFAGYAAGIAAYLALHNLNISLPTTIAEVPFILG 581
           RKVSGFAFAGYAAGIAAYLALHNLN SLPTTIAEVP I+G
Sbjct: 541 RKVSGFAFAGYAAGIAAYLALHNLNTSLPTTIAEVPSIMG 575

BLAST of Moc10g04530 vs. ExPASy TrEMBL
Match: B9RYA4 (Na+/H+ antiporter OS=Ricinus communis OX=3988 GN=RCOM_0811580 PE=2 SV=1)

HSP 1 Score: 905.2 bits (2338), Expect = 1.4e-259
Identity = 481/585 (82.22%), Postives = 527/585 (90.09%), Query Frame = 0

Query: 1   MASLSIGIH-LPPSHHWKRFSLQSLSPI---SLPFHTRYASSSSRLRTPRLLRKNLLARA 60
           MASLSIGIH   P+H +K+ S+   S     SLP  + ++SS  RLR  RLLR   LARA
Sbjct: 1   MASLSIGIHNFNPTHQFKKHSIDLPSSFYLSSLPSQSCFSSSLHRLRRSRLLRSGFLARA 60

Query: 61  EDEAKGSSPASSSSFQQQPAPPGFSNQLEEFTSKSGTCDPLCSVDEISSQDFEASYQPKT 120
           ED+AKG     +SS+Q Q  PP    QL+E T  +G+CDPLCS+D+ SSQDFEASYQPKT
Sbjct: 61  EDKAKG-----TSSYQGQAQPPNTVKQLQELTPDAGSCDPLCSLDDTSSQDFEASYQPKT 120

Query: 121 DLLKAFAIFAAAITGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLLM 180
           DL+KA A+FAAA TG  AINHSWVAANQDLAMVLLF IGYAGIIFEESLAFNKSGVGLLM
Sbjct: 121 DLVKALAVFAAAATGAAAINHSWVAANQDLAMVLLFGIGYAGIIFEESLAFNKSGVGLLM 180

Query: 181 AVGLWVVRSIGAPSTEIAVSELTHSTAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSIT 240
           AV LWVVRSIGAPST+IAVSELTH++AEVSEIVFFLLGAMTIVEIVDAHQGFKLVTD+IT
Sbjct: 181 AVSLWVVRSIGAPSTDIAVSELTHASAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDNIT 240

Query: 241 TRKPRTLLWVIGGVTFFLSSILDNLTATIIMVSLLRKLVPPSEYRKLLGAVIVIAANAGG 300
           TRKPRTLLWV+G VTFFLSSILDNLT+TI+MVSLLRKLVPPSEYRKLLGAV+VIAANAGG
Sbjct: 241 TRKPRTLLWVVGFVTFFLSSILDNLTSTIVMVSLLRKLVPPSEYRKLLGAVVVIAANAGG 300

Query: 301 AWTPIGDVTTTMLWIHGQISTVQTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNVM 360
           AWTPIGDVTTTMLWIHGQIST+ T+K L++PSAVSLAVPLAL+SLTSEVN K      V+
Sbjct: 301 AWTPIGDVTTTMLWIHGQISTLPTMKILLIPSAVSLAVPLALMSLTSEVNGKEHGSSKVL 360

Query: 361 ASEQMAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESERQ 420
           ASE+MAPRG+LVFSVG+GAL+FVPVFKALTGLPPYMGMLLGLG+LWILTDAIHYGESERQ
Sbjct: 361 ASERMAPRGQLVFSVGIGALIFVPVFKALTGLPPYMGMLLGLGVLWILTDAIHYGESERQ 420

Query: 421 KLKVPQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVVS 480
           KLKVPQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHI+N EL+ASA+GVVS
Sbjct: 421 KLKVPQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHISNVELVASAIGVVS 480

Query: 481 AIIDNVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVDF 540
           AIIDNVPLVAATMGMYDL+SFP+DSEFWQLIA+CAGTGGSML+IGSAAGVAFMGMEKVDF
Sbjct: 481 AIIDNVPLVAATMGMYDLTSFPQDSEFWQLIAYCAGTGGSMLVIGSAAGVAFMGMEKVDF 540

Query: 541 FWYLRKVSGFAFAGYAAGIAAYLALHNLNISLPTTIAEVPFILGS 582
           FWYLRKVSGFAFAGYAAGIAAYLA+ NLNISLPTT+AEVPF+ GS
Sbjct: 541 FWYLRKVSGFAFAGYAAGIAAYLAVRNLNISLPTTLAEVPFLSGS 580

BLAST of Moc10g04530 vs. TAIR 10
Match: AT3G19490.1 (sodium:hydrogen antiporter 1 )

HSP 1 Score: 844.0 bits (2179), Expect = 7.4e-245
Identity = 452/586 (77.13%), Postives = 503/586 (85.84%), Query Frame = 0

Query: 1   MASLSIGIHLPPSHH-WKRFSLQSLSPIS----LPFHTRYASSSSRLRTPRLLRKNLLAR 60
           MA   IG H  P H   KR  + + SPIS    LP +  ++  S    + RL +  +L R
Sbjct: 1   MAVFPIGSHFAPPHQLTKRHVIATSSPISISTRLPQNVSFSKVSGVTGSTRLSKHGVLVR 60

Query: 61  AEDEAKGSSPASSSSFQQQPAPPGFSNQLEEFTSKSGTCDPLCSVDEISSQDFEASYQPK 120
           AED+ + SS  SS     +P         E+    SG CDPLCSVDE SS  FEA+YQPK
Sbjct: 61  AEDKIRSSSSPSS---LDEPID-------EDLMDSSGLCDPLCSVDEPSSSYFEANYQPK 120

Query: 121 TDLLKAFAIFAAAITGTVAINHSWVAANQDLAMVLLFSIGYAGIIFEESLAFNKSGVGLL 180
           TD++KA AI AAA+TGT AINHSWVAANQD+AM LLF IGYAGIIFEESLAFNKSG+GLL
Sbjct: 121 TDIIKALAILAAALTGTAAINHSWVAANQDVAMALLFGIGYAGIIFEESLAFNKSGIGLL 180

Query: 181 MAVGLWVVRSIGAPSTEIAVSELTHSTAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDSI 240
           MAV LWVVRSIGAPSTEIAV +L H+TAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTD+I
Sbjct: 181 MAVSLWVVRSIGAPSTEIAVLDLQHATAEVSEIVFFLLGAMTIVEIVDAHQGFKLVTDNI 240

Query: 241 TTRKPRTLLWVIGGVTFFLSSILDNLTATIIMVSLLRKLVPPSEYRKLLGAVIVIAANAG 300
           TTRKP+TLLWV+G VTFFLSSILDNLT+TI+MVSL+RKLVP SEYRKLLG V+VIAANAG
Sbjct: 241 TTRKPKTLLWVVGFVTFFLSSILDNLTSTIVMVSLIRKLVPQSEYRKLLGGVVVIAANAG 300

Query: 301 GAWTPIGDVTTTMLWIHGQISTVQTVKDLVVPSAVSLAVPLALLSLTSEVNVKGQEFPNV 360
           GAWTPIGDVTTTMLWIHGQIST+ T+KDL +PS VSLAVPLAL+SLTSEVN K Q+  +V
Sbjct: 301 GAWTPIGDVTTTMLWIHGQISTLPTMKDLFLPSVVSLAVPLALMSLTSEVNGKEQDPKDV 360

Query: 361 MASEQMAPRGKLVFSVGVGALVFVPVFKALTGLPPYMGMLLGLGILWILTDAIHYGESER 420
           +ASE+MAPRGKLVF VG+GALVFVPVFKALTGLPPYMG+LLGLG+LWILTDAIHYGESER
Sbjct: 361 LASEKMAPRGKLVFGVGLGALVFVPVFKALTGLPPYMGILLGLGVLWILTDAIHYGESER 420

Query: 421 QKLKVPQALSRIDTQGALFFLGILLSVSSLEAAGILRELANYLDAHIANAELIASAVGVV 480
           QKLKVPQALSRIDTQGALFFLGILLSVSSLEAAGILRE+ANYLDA+I N ELIASA+GVV
Sbjct: 421 QKLKVPQALSRIDTQGALFFLGILLSVSSLEAAGILREIANYLDANIPNVELIASAIGVV 480

Query: 481 SAIIDNVPLVAATMGMYDLSSFPRDSEFWQLIAFCAGTGGSMLIIGSAAGVAFMGMEKVD 540
           SAIIDNVPLVAATMGMYDL+SFP+DSEFWQLIAFCAGTGGSML+IGSAAGVAFMGMEKVD
Sbjct: 481 SAIIDNVPLVAATMGMYDLTSFPQDSEFWQLIAFCAGTGGSMLVIGSAAGVAFMGMEKVD 540

Query: 541 FFWYLRKVSGFAFAGYAAGIAAYLALHNLNISLPTTIAEVPFILGS 582
           FFWY RKVSGFAFAGYAAGIAAYLA+HNL+  +PTT+A++PF+ GS
Sbjct: 541 FFWYFRKVSGFAFAGYAAGIAAYLAVHNLHFEIPTTVAQIPFLTGS 576

BLAST of Moc10g04530 vs. TAIR 10
Match: AT1G49810.1 (Na+/H+ antiporter 2 )

HSP 1 Score: 623.6 bits (1607), Expect = 1.6e-178
Identity = 322/418 (77.03%), Postives = 369/418 (88.28%), Query Frame = 0

Query: 148 MVLLFSIGYAGIIFEESLAFNKSGVGLLMAVGLWVVRSIGAPSTEIAVSELTHSTAEVSE 207
           M LLF IGY  IIFEESL+F+KSG+ LLMAV LWVVRSI   S EI   EL H+T+EVS+
Sbjct: 1   MALLFGIGYVLIIFEESLSFSKSGIALLMAVSLWVVRSI-ETSVEIVTLELQHATSEVSQ 60

Query: 208 IVFFLLGAMTIVEIVDAHQGFKLVTDSITTRKPRTLLWVIGGVTFFLSSILDNLTATIIM 267
           IVF++LGAMTIVEI+DAHQGFKLVTD IT+RKP+ LLWVIG  TFFLSS+LDNLT+TI+M
Sbjct: 61  IVFYMLGAMTIVEIIDAHQGFKLVTDCITSRKPKILLWVIGFATFFLSSVLDNLTSTIVM 120

Query: 268 VSLLRKLVPPSEYRKLLGAVIVIAANAGGAWTPIGDVTTTMLWIHGQISTVQTVKDLVVP 327
           VSLLR+L+PPSEYRKLLGAV+VIAANAGGAWTPIGDVTTTMLWIHG IST  T+K+L +P
Sbjct: 121 VSLLRRLIPPSEYRKLLGAVVVIAANAGGAWTPIGDVTTTMLWIHGHISTFSTIKNLFLP 180

Query: 328 SAVSLAVPLALLSLTSEVNVKGQEFP--NVMASEQMAPRGKLVFSVGVGALVFVPVFKAL 387
           SA+SL VPLAL+SLTSEV+  G   P   ++A ++ APRGKLVF VG GAL+FVP+FK+L
Sbjct: 181 SAISLVVPLALMSLTSEVHGMGLNTPPTPLLAYDRSAPRGKLVFGVGFGALLFVPLFKSL 240

Query: 388 TGLPPYMGMLLGLGILWILTDAIHYGESERQKLKVPQALSRIDTQGALFFLGILLSVSSL 447
           TGLPPYMG+LLGLG++WILTD IHYG+ ERQ LK+P ALSRID+QGALFFLGILLS+SSL
Sbjct: 241 TGLPPYMGILLGLGVIWILTDVIHYGDLERQHLKLPHALSRIDSQGALFFLGILLSMSSL 300

Query: 448 EAAGILRELANYLDAHIANAELIASAVGVVSAIIDNVPLVAATMGMYDLSSFPRDSEFWQ 507
           +AAGIL+ +ANYLDAHIAN ELIAS +GVVSAIIDNVPLVAATMGMYDLS+FP+DSEFWQ
Sbjct: 301 DAAGILKVIANYLDAHIANVELIASIIGVVSAIIDNVPLVAATMGMYDLSTFPQDSEFWQ 360

Query: 508 LIAFCAGTGGSMLIIGSAAGVAFMGMEKVDFFWYLRKVSGFAFAGYAAGIAAYLALHN 564
           LI+FCAGTGGSMLI GSAAGV FM MEKV+FFWY RKVSGFAFAG+ AGI  YLA+HN
Sbjct: 361 LISFCAGTGGSMLITGSAAGVIFMSMEKVNFFWYFRKVSGFAFAGFTAGIMTYLAVHN 417

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022149216.10.0e+00100.00sodium/proton antiporter 1 [Momordica charantia][more]
XP_038880556.16.8e-29391.74sodium/proton antiporter 1 [Benincasa hispida][more]
XP_004140712.12.4e-29091.74sodium/proton antiporter 1 [Cucumis sativus][more]
XP_008456161.15.4e-29091.57PREDICTED: Na(+)/H(+) antiporter NhaD [Cucumis melo][more]
KAG6576940.12.0e-28490.69Sodium/proton antiporter 1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9LT681.0e-24377.13Sodium/proton antiporter 1 OS=Arabidopsis thaliana OX=3702 GN=NHD1 PE=2 SV=1[more]
Q9C6D32.2e-17777.03Sodium/proton antiporter 2 OS=Arabidopsis thaliana OX=3702 GN=NHD2 PE=3 SV=1[more]
A5F1205.0e-3630.67Na(+)/H(+) antiporter NhaD OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / C... [more]
Q56EB31.1e-3530.13Na(+)/H(+) antiporter NhaD OS=Alkalimonas amylolytica OX=152573 GN=nhaD PE=1 SV=... [more]
E1VBT74.8e-3129.87Na(+)/H(+) antiporter NhaD OS=Halomonas elongata (strain ATCC 33173 / DSM 2581 /... [more]
Match NameE-valueIdentityDescription
A0A6J1D7Q20.0e+00100.00sodium/proton antiporter 1 OS=Momordica charantia OX=3673 GN=LOC111017691 PE=4 S... [more]
A0A1S3C2P82.6e-29091.57Na(+)/H(+) antiporter NhaD OS=Cucumis melo OX=3656 GN=LOC103496182 PE=4 SV=1[more]
A0A6J1JA421.6e-28490.69sodium/proton antiporter 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482646 PE=4... [more]
A0A6J1E3Q72.1e-28490.52sodium/proton antiporter 1-like OS=Cucurbita moschata OX=3662 GN=LOC111430554 PE... [more]
B9RYA41.4e-25982.22Na+/H+ antiporter OS=Ricinus communis OX=3988 GN=RCOM_0811580 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G19490.17.4e-24577.13sodium:hydrogen antiporter 1 [more]
AT1G49810.11.6e-17877.03Na+/H+ antiporter 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004680Citrate transporter-like domainPFAMPF03600CitMHScoord: 157..512
e-value: 4.7E-27
score: 94.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 62..85
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 58..85
NoneNo IPR availablePANTHERPTHR43269:SF3BNAC03G40960D PROTEINcoord: 1..581
IPR045016Na(+)/H(+) antiporter NhaD-likePANTHERPTHR43269SODIUM/PROTON ANTIPORTER 1-RELATEDcoord: 1..581

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc10g04530.1Moc10g04530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006814 sodium ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015297 antiporter activity