Moc09g41680 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g41680
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description(+)-neomenthol dehydrogenase-like
Locationchr9: 31713743 .. 31716111 (-)
RNA-Seq ExpressionMoc09g41680
SyntenyMoc09g41680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAAATTCTCGCTCTCAAATCTCATCGTCCATGGATTTGGATTCCAAGGAATTCGACCTCTCTTCCCCTTCTTCTTCGCCCTTCTCCCACAGGTAATTCACTCTCAAATCACAACTAATTTCATTTCATTGTGATCTAATTTAATTAATTCACTTTCCCGGCCGGCTGCCGTTGCAGATGGTGGTCCAAGAACACGGTGGCCGTCGTCACCGGAGCCAATAAGGGGATCGGATTTGCCCTAGTCAGGAGATTAGCGCAATTGGAACTCACCGTCATTTTAACAGCCAGGGATCAAGAAAGAGGATTCAACGCCGTCCAGACCCTGCGAAATGAAGGACTCCCCGTTCTCTTCCGCCGCCTCGATGTTTCCGATCCCCTCTCCATCGCCTCCTTCGCCGCCTGGTTCACCGCCAATTTTCAAGCCCTCGACATTCTCGTGAGTACTTTTTTCCCTTCATTCTCTCCCGTTTCTTTTTCTCATTTTGCAATTTCAACCCTCCACTTACTTCAAATTACAATTTGTTTTTTTCCTTCACGTTGATTTATTTTAGTTTACACGTGTTCTGAAATCTTGATCAATTTTTGGAAGCTGACAAAATTATTTTCTTTTAAATGCTATTTTGGTGTCTTAATTCGTACACTTAGTTTTGTTTAATACTTTATTCCTTCAGTTTTTTTTTTCCTTCTAATTTTACAACTTTGCATGCGGGGAATGTGACATGTTACAATACAATTTGAAATATGAAAGATCTATATATATTTTTTAATCTTTGAAATATGTAGTTTAAGCGACATAGAAACTTGTGATTTAAGATCCATAGAAGATCCCTGAGTGGAGAACATGATTTAATTGTGTTTTTGAGTTCCGTTGCCAAAAAAATTAAAATTAAAATTAACATGTTAGTACCTTCTTCTTTAAAAGGTTGAATCTCCTATGAGAAATTTTACTACATATCGTAAATTTAATACTCATTATAAAAAGTATCACAAATTTTTTTCTTTTTCATTTTTTAAAAACGTGCTATTTTGGTCAATTTTTACACTTTCTACCACATTTATAAATAGTATTACTCTCATTTAATTGTAATGCTATATTCTCAAAACATAAATCAGAATGCTAATTTGAAATTAGAAAAGAAAGTAAAAGAATTATTAGGAAACGATCTCATGTTTGTGAAGTAAAAATTGAAAGAAAAAAAAAGGCCAGGAGGGACTTTGATAATTAGTGAAATTAATTTGGGTTTAGTAAGGGGTTTGTCGTTGAGTTAGTTTCATTGATGAAGACCTATTGGTAGGTATGCTACGTGTATTTATGAAAGGTATATCTAATCATGATCTTATTTTTATTTTTTTAATATTTTAAAAGAAGACCATATAGTCTTCGGAAAGCAGAATATCGTGTACTAAAAAAAAAAAAAAAAAAAGCAAGTCAATGTGAAAGAAGAAAAGTAAGATTTACGTTTTTGCAAGAAAAAGGCGAACCAGTGATTGGTTTGGATGTTCCCTCCGGGTCCCTCCCACTGAATTTGGCTTGGATGTTCCTATTTTGTAGTGGAGCTGAGTTCGAGTGTGGAGAAACACCGATTTCATACCCCTGTTTCTTCTTCATATTATATTGTCTAATTTTTTAATTATACAAATTACAAAATTAGTTGAATTAATTTGCAGGTGAACAACGCGGCCGTATCGTTCAACGACATAAACGAGAACTCGGTGGCAAACGCAGAAACGGTAATGAAAACAAACTATTACGGCGCGAAATTGCTGACCCAGGCTCTATTGCCTGCCTTCCGCTCCTCTGCGCCTTCCAGGGCCCGAATTCTCAATATCACCTCAAGGCTTGGGACCATCGATGTAGGAGCTTGCTTAATTAATTGGCTACTTTATTAATTTGAAATTAAAAAAAAAAAAAAAGAGATGAATTAATTGATGATAATTTTGAATATTGGTTGGTGCAGAAGGTGAAGAACGTGAAAGTGAGAGAGATTCTGGGGAGTGAAGATGTGGGGGAGGAGGATATAGAAGGCGTGGTGAATGCGTTTCTGGGGGACGTGAAGAGAGGGACCTGGAAGAAGGAGGGTTGGCCGGCGGTGTGGACGGAGTATGCGGTCTCCAAGCTGGCCCTCAACGCCTACACCGCTGTTCTGGCCAAGCGCTTCGCCCAGTTTGGGACTAGTGTGAGCGTCAACAGCTTCTGCCCTGGCTTCACCCAGACCTCCATGACTCGTGGGAAGGGCACCCACACCGCCGAAGCCGCCGCCGCCGTCGGAGCTCGCCTCGCCTTGCTGCCTCCCCACCGCCTCCCCACCGGCCACTTCTTCTCCTGGGGCCCCAACTATGATGTACCCAAAAACTCCAGATTATAA

mRNA sequence

ATGCCAAATTCTCGCTCTCAAATCTCATCGTCCATGGATTTGGATTCCAAGGAATTCGACCTCTCTTCCCCTTCTTCTTCGCCCTTCTCCCACAGATGGTGGTCCAAGAACACGGTGGCCGTCGTCACCGGAGCCAATAAGGGGATCGGATTTGCCCTAGTCAGGAGATTAGCGCAATTGGAACTCACCGTCATTTTAACAGCCAGGGATCAAGAAAGAGGATTCAACGCCGTCCAGACCCTGCGAAATGAAGGACTCCCCGTTCTCTTCCGCCGCCTCGATGTTTCCGATCCCCTCTCCATCGCCTCCTTCGCCGCCTGGTTCACCGCCAATTTTCAAGCCCTCGACATTCTCGTGAACAACGCGGCCGTATCGTTCAACGACATAAACGAGAACTCGGTGGCAAACGCAGAAACGGTAATGAAAACAAACTATTACGGCGCGAAATTGCTGACCCAGGCTCTATTGCCTGCCTTCCGCTCCTCTGCGCCTTCCAGGGCCCGAATTCTCAATATCACCTCAAGGCTTGGGACCATCGATAAGGTGAAGAACGTGAAAGTGAGAGAGATTCTGGGGAGTGAAGATGTGGGGGAGGAGGATATAGAAGGCGTGGTGAATGCGTTTCTGGGGGACGTGAAGAGAGGGACCTGGAAGAAGGAGGGTTGGCCGGCGGTGTGGACGGAGTATGCGGTCTCCAAGCTGGCCCTCAACGCCTACACCGCTGTTCTGGCCAAGCGCTTCGCCCAGTTTGGGACTAGTGTGAGCGTCAACAGCTTCTGCCCTGGCTTCACCCAGACCTCCATGACTCGTGGGAAGGGCACCCACACCGCCGAAGCCGCCGCCGCCGTCGGAGCTCGCCTCGCCTTGCTGCCTCCCCACCGCCTCCCCACCGGCCACTTCTTCTCCTGGGGCCCCAACTATGATGTACCCAAAAACTCCAGATTATAA

Coding sequence (CDS)

ATGCCAAATTCTCGCTCTCAAATCTCATCGTCCATGGATTTGGATTCCAAGGAATTCGACCTCTCTTCCCCTTCTTCTTCGCCCTTCTCCCACAGATGGTGGTCCAAGAACACGGTGGCCGTCGTCACCGGAGCCAATAAGGGGATCGGATTTGCCCTAGTCAGGAGATTAGCGCAATTGGAACTCACCGTCATTTTAACAGCCAGGGATCAAGAAAGAGGATTCAACGCCGTCCAGACCCTGCGAAATGAAGGACTCCCCGTTCTCTTCCGCCGCCTCGATGTTTCCGATCCCCTCTCCATCGCCTCCTTCGCCGCCTGGTTCACCGCCAATTTTCAAGCCCTCGACATTCTCGTGAACAACGCGGCCGTATCGTTCAACGACATAAACGAGAACTCGGTGGCAAACGCAGAAACGGTAATGAAAACAAACTATTACGGCGCGAAATTGCTGACCCAGGCTCTATTGCCTGCCTTCCGCTCCTCTGCGCCTTCCAGGGCCCGAATTCTCAATATCACCTCAAGGCTTGGGACCATCGATAAGGTGAAGAACGTGAAAGTGAGAGAGATTCTGGGGAGTGAAGATGTGGGGGAGGAGGATATAGAAGGCGTGGTGAATGCGTTTCTGGGGGACGTGAAGAGAGGGACCTGGAAGAAGGAGGGTTGGCCGGCGGTGTGGACGGAGTATGCGGTCTCCAAGCTGGCCCTCAACGCCTACACCGCTGTTCTGGCCAAGCGCTTCGCCCAGTTTGGGACTAGTGTGAGCGTCAACAGCTTCTGCCCTGGCTTCACCCAGACCTCCATGACTCGTGGGAAGGGCACCCACACCGCCGAAGCCGCCGCCGCCGTCGGAGCTCGCCTCGCCTTGCTGCCTCCCCACCGCCTCCCCACCGGCCACTTCTTCTCCTGGGGCCCCAACTATGATGTACCCAAAAACTCCAGATTATAA

Protein sequence

MPNSRSQISSSMDLDSKEFDLSSPSSSPFSHRWWSKNTVAVVTGANKGIGFALVRRLAQLELTVILTARDQERGFNAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVNNAAVSFNDINENSVANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTIDKVKNVKVREILGSEDVGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRFAQFGTSVSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHFFSWGPNYDVPKNSRL
Homology
BLAST of Moc09g41680 vs. NCBI nr
Match: XP_022151760.1 ((+)-neomenthol dehydrogenase-like [Momordica charantia])

HSP 1 Score: 616.3 bits (1588), Expect = 1.5e-172
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0

Query: 1   MPNSRSQISSSMDLDSKEFDLSSPSSSPFSHRWWSKNTVAVVTGANKGIGFALVRRLAQL 60
           MPNSRSQISSSMDLDSKEFDLSSPSSSPFSHRWWSKNTVAVVTGANKGIGFALVRRLAQL
Sbjct: 1   MPNSRSQISSSMDLDSKEFDLSSPSSSPFSHRWWSKNTVAVVTGANKGIGFALVRRLAQL 60

Query: 61  ELTVILTARDQERGFNAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVN 120
           ELTVILTARDQERGFNAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVN
Sbjct: 61  ELTVILTARDQERGFNAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVN 120

Query: 121 NAAVSFNDINENSVANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTID 180
           NAAVSFNDINENSVANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTID
Sbjct: 121 NAAVSFNDINENSVANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTID 180

Query: 181 KVKNVKVREILGSEDVGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYT 240
           KVKNVKVREILGSEDVGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYT
Sbjct: 181 KVKNVKVREILGSEDVGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYT 240

Query: 241 AVLAKRFAQFGTSVSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHF 300
           AVLAKRFAQFGTSVSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHF
Sbjct: 241 AVLAKRFAQFGTSVSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHF 300

Query: 301 FSWGPNYDVPKNSRL 316
           FSWGPNYDVPKNSRL
Sbjct: 301 FSWGPNYDVPKNSRL 315

BLAST of Moc09g41680 vs. NCBI nr
Match: XP_038891404.1 ((+)-neomenthol dehydrogenase-like [Benincasa hispida])

HSP 1 Score: 488.8 bits (1257), Expect = 3.5e-134
Identity = 248/300 (82.67%), Postives = 275/300 (91.67%), Query Frame = 0

Query: 16  SKEFDLSSPSSSPFSHRWWSKNTVAVVTGANKGIGFALVRRLAQLELTVILTARDQERGF 75
           SKEFDL   SSSPFS RWWSKN+VA+VTGANKGIGFALVR+LAQ +LTVILTARD+ RG 
Sbjct: 3   SKEFDL---SSSPFSERWWSKNSVAIVTGANKGIGFALVRKLAQSDLTVILTARDEGRGL 62

Query: 76  NAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVNNAAVSFNDINENSVA 135
            AV+TLRNEGL VLFRRLDVSDP+SI +FAAWF +NFQALDILVNNAAVSFNDI+ENSV 
Sbjct: 63  KAVETLRNEGLHVLFRRLDVSDPVSINAFAAWFASNFQALDILVNNAAVSFNDIHENSVE 122

Query: 136 NAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTIDKVKNVKVREILGSED 195
           NAETVMKTN+YGAKLLT+ALLP FRSSA S++RILNITSRLGT+DKV+N+KV+EIL SED
Sbjct: 123 NAETVMKTNFYGAKLLTEALLPYFRSSA-SKSRILNITSRLGTVDKVRNMKVKEILESED 182

Query: 196 VGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRFAQFGTSVS 255
           VGEEDIEGVVNAFL DVKRGTWKK GWP +WT+YAVSKLALN YT VLAKR+ ++G SVS
Sbjct: 183 VGEEDIEGVVNAFLKDVKRGTWKKAGWPTLWTDYAVSKLALNTYTRVLAKRYGEYG-SVS 242

Query: 256 VNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHFFSWGPNYDVPKNSRL 315
           VNSFCPGFTQTSMTRGKGTHTA+AAA VGARLALLPPHRLPTG FF WGPNYD+P+NS+L
Sbjct: 243 VNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPHRLPTGQFFFWGPNYDIPRNSKL 297

BLAST of Moc09g41680 vs. NCBI nr
Match: XP_023542237.1 ((+)-neomenthol dehydrogenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 486.9 bits (1252), Expect = 1.3e-133
Identity = 249/302 (82.45%), Postives = 271/302 (89.74%), Query Frame = 0

Query: 14  LDSKEFDLSSPSSSPFSHRWWSKNTVAVVTGANKGIGFALVRRLAQLELTVILTARDQER 73
           +DSKEFDLSS SSSPFS RWWS NTVAVVTGANKGIGFALVRRLAQ+ELTVILT R++ R
Sbjct: 1   MDSKEFDLSSASSSPFSDRWWSNNTVAVVTGANKGIGFALVRRLAQMELTVILTTRNEGR 60

Query: 74  GFNAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVNNAAVSFNDINENS 133
           G  AV++LRNEGL VLFRRLDVSDP SI +FAAWF ANF ALDILVNNAAVSFNDI+ NS
Sbjct: 61  GLKAVESLRNEGLNVLFRRLDVSDPASINAFAAWFVANFPALDILVNNAAVSFNDIDHNS 120

Query: 134 VANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTIDKVKNVKVREILGS 193
           V NAETV+KTN+YGAKLLTQALLP FRSS+ SR+RILNITSRLGTIDKVKN KV+EIL S
Sbjct: 121 VENAETVIKTNFYGAKLLTQALLPLFRSSSSSRSRILNITSRLGTIDKVKNEKVKEILQS 180

Query: 194 EDVGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRFAQFGTS 253
           ED+ EEDI+GVV+AFL DVKRGTWKK+GWPA+WT+YAVSKLALNAYT VLAKR      S
Sbjct: 181 EDLAEEDIDGVVDAFLQDVKRGTWKKKGWPALWTDYAVSKLALNAYTRVLAKR----SRS 240

Query: 254 VSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHFFSWGPNYDVPKNS 313
           VSVNSFCPGFTQTSMT GKGTHTA+AAA VGARLALLPPHRLPTG FFSWGPNYDVP+ S
Sbjct: 241 VSVNSFCPGFTQTSMTGGKGTHTADAAAVVGARLALLPPHRLPTGQFFSWGPNYDVPRKS 298

Query: 314 RL 316
           +L
Sbjct: 301 KL 298

BLAST of Moc09g41680 vs. NCBI nr
Match: XP_022966808.1 ((+)-neomenthol dehydrogenase-like [Cucurbita maxima])

HSP 1 Score: 485.3 bits (1248), Expect = 3.9e-133
Identity = 248/302 (82.12%), Postives = 270/302 (89.40%), Query Frame = 0

Query: 14  LDSKEFDLSSPSSSPFSHRWWSKNTVAVVTGANKGIGFALVRRLAQLELTVILTARDQER 73
           +DSKEFDLSS SSSPFS RWWS NTVAVVTGANKGIGFALVRRLAQ+ELTVILT R++ R
Sbjct: 1   MDSKEFDLSSASSSPFSDRWWSNNTVAVVTGANKGIGFALVRRLAQMELTVILTTRNEGR 60

Query: 74  GFNAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVNNAAVSFNDINENS 133
           G  AV++LRNEGL V FRRLDVSDP SI +FAAWF ANF ALDILVNNAAVSFNDI+ NS
Sbjct: 61  GLKAVESLRNEGLNVRFRRLDVSDPASINAFAAWFVANFPALDILVNNAAVSFNDIDHNS 120

Query: 134 VANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTIDKVKNVKVREILGS 193
           V NAETV+KTN+YGAKLLTQALLP FRSS+ SR+RILNITSRLGTIDKVKN KV+EIL S
Sbjct: 121 VENAETVIKTNFYGAKLLTQALLPLFRSSSSSRSRILNITSRLGTIDKVKNEKVKEILQS 180

Query: 194 EDVGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRFAQFGTS 253
           ED+ EEDI+GVV+AFL DVKRGTWKK+GWPA+WT+YAVSKLALNAYT VLAKR      S
Sbjct: 181 EDLAEEDIDGVVDAFLQDVKRGTWKKKGWPALWTDYAVSKLALNAYTRVLAKR----SRS 240

Query: 254 VSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHFFSWGPNYDVPKNS 313
           VSVNSFCPGFTQTSMT GKGTHTA+AAA VGARLALLPPHRLPTG FFSWGPNYD P+NS
Sbjct: 241 VSVNSFCPGFTQTSMTGGKGTHTADAAAVVGARLALLPPHRLPTGQFFSWGPNYDTPRNS 298

Query: 314 RL 316
           +L
Sbjct: 301 KL 298

BLAST of Moc09g41680 vs. NCBI nr
Match: KAG6573598.1 ((+)-neomenthol dehydrogenase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012680.1 (+)-neomenthol dehydrogenase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 481.9 bits (1239), Expect = 4.3e-132
Identity = 248/302 (82.12%), Postives = 271/302 (89.74%), Query Frame = 0

Query: 14  LDSKEFDLSSPSSSPFSHRWWSKNTVAVVTGANKGIGFALVRRLAQLELTVILTARDQER 73
           +DSKEFDLSS SSSPFS+RWWS NTVAVVTGANKGIGFALVRRLAQ+ELTVILT R++ R
Sbjct: 1   MDSKEFDLSSASSSPFSNRWWSNNTVAVVTGANKGIGFALVRRLAQMELTVILTTRNEGR 60

Query: 74  GFNAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVNNAAVSFNDINENS 133
           G  AV++LRNEGL VLFRRLDVSDP SI +FAAWF ANF ALDILVNNAAVSFNDI+ NS
Sbjct: 61  GLKAVESLRNEGLNVLFRRLDVSDPASINAFAAWFVANFPALDILVNNAAVSFNDIDHNS 120

Query: 134 VANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTIDKVKNVKVREILGS 193
           V NAETV+KTN+YGAKLLTQALLP FRSS+ SR+RILNITSRLGTIDKVKN KV+EIL S
Sbjct: 121 VENAETVIKTNFYGAKLLTQALLPLFRSSS-SRSRILNITSRLGTIDKVKNEKVKEILQS 180

Query: 194 EDVGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRFAQFGTS 253
           ED+ EEDI+GVV+AFL DVKRGTWKK+GWPA+WT+YAVSKLALNAYT VLAKR      S
Sbjct: 181 EDLAEEDIDGVVDAFLQDVKRGTWKKKGWPALWTDYAVSKLALNAYTRVLAKR----SRS 240

Query: 254 VSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHFFSWGPNYDVPKNS 313
           VSVNSFCPGFTQTSMT GKGTHTA+AAA VGARLALLPPHRLPTG FF WGPNYD P+NS
Sbjct: 241 VSVNSFCPGFTQTSMTGGKGTHTADAAAVVGARLALLPPHRLPTGQFFYWGPNYDTPRNS 297

Query: 314 RL 316
           +L
Sbjct: 301 KL 297

BLAST of Moc09g41680 vs. ExPASy Swiss-Prot
Match: Q9M2E2 ((+)-neomenthol dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=SDR1 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.2e-44
Identity = 114/287 (39.72%), Postives = 159/287 (55.40%), Query Frame = 0

Query: 40  AVVTGANKGIGFALVRRLAQLELTVILTARDQERGFNAVQTLRNE----GLPVLFRRLDV 99
           AVVTGAN+GIGF + R+LA   + V+LT+RD+ RG  AV+TL+ E       +LF +LDV
Sbjct: 9   AVVTGANRGIGFEICRQLASEGIRVVLTSRDENRGLEAVETLKKELEISDQSLLFHQLDV 68

Query: 100 SDPLSIASFAAWFTANFQALDILVNNAAV-------------------SFNDINENSVAN 159
           +DP SI S A +    F  LDILVNNA +                    +++I   +   
Sbjct: 69  ADPASITSLAEFVKTQFGKLDILVNNAGIGGIITDAEALRAGAGKEGFKWDEIITETYEL 128

Query: 160 AETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTIDKVKNVKVREILG-SED 219
            E  +K NYYG K + +A +P  + S     RI+N++S +G +  V N   + IL  +E+
Sbjct: 129 TEECIKINYYGPKRMCEAFIPLLKLS--DSPRIVNVSSSMGQLKNVLNEWAKGILSDAEN 188

Query: 220 VGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRFAQFGTSVS 279
           + EE I+ V+N  L D K GT K++ W    + Y VSK +LN YT VLAK+  +F     
Sbjct: 189 LTEERIDQVINQLLNDFKEGTVKEKNWAKFMSAYVVSKASLNGYTRVLAKKHPEF----R 248

Query: 280 VNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHFFS 303
           VN+ CPGF +T M    G  + E  A+   RLALLP    P+G FFS
Sbjct: 249 VNAVCPGFVKTDMNFKTGVLSVEEGASSPVRLALLPHQETPSGCFFS 289

BLAST of Moc09g41680 vs. ExPASy Swiss-Prot
Match: Q9ZUH5 (Short-chain dehydrogenase/reductase 2b OS=Arabidopsis thaliana OX=3702 GN=SDR2b PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 4.3e-42
Identity = 110/286 (38.46%), Postives = 161/286 (56.29%), Query Frame = 0

Query: 40  AVVTGANKGIGFALVRRLAQLELTVILTARDQERGFNAVQTLRNE----GLPVLFRRLDV 99
           A+VTG N+GIGF + R+LA   + VILT+RD+++G  AV+TL+ E       ++F +LDV
Sbjct: 9   AIVTGGNRGIGFEICRQLANKGIRVILTSRDEKQGLEAVETLKKELEISDQSIVFHQLDV 68

Query: 100 SDPLSIASFAAWFTANFQALDILVNNAAVS--FNDIN-------------ENSVAN---- 159
           SDP+S+ S A +   +F  LDIL+NNA V     D++             E ++      
Sbjct: 69  SDPVSVTSLAEFVKTHFGKLDILINNAGVGGVITDVDALRAGTGKEGFKWEETITETYEL 128

Query: 160 AETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTIDKVKNVKVREILG-SED 219
           AE  +K NYYG K + +A +P  + S     RI+N++S +G +  + N   + IL  +E+
Sbjct: 129 AEECIKINYYGPKRMCEAFIPLLQLS--DSPRIINVSSFMGQVKNLVNEWAKGILSDAEN 188

Query: 220 VGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRFAQFGTSVS 279
           + E  I+ V+N  L D+K  T K + W  V + Y VSK  LNAYT +LAK+  +    + 
Sbjct: 189 LTEVRIDQVINQLLNDLKEDTAKTKYWAKVMSAYVVSKAGLNAYTRILAKKHPE----IR 248

Query: 280 VNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHFF 302
           VNS CPGF +T M    G  + E  A+   RLALLP    P+G FF
Sbjct: 249 VNSVCPGFVKTDMNFKTGILSVEEGASSPVRLALLPHQESPSGCFF 288

BLAST of Moc09g41680 vs. ExPASy Swiss-Prot
Match: A4UHT7 (Salutaridine reductase OS=Papaver bracteatum OX=215227 GN=SALR PE=1 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.4e-40
Identity = 117/301 (38.87%), Postives = 158/301 (52.49%), Query Frame = 0

Query: 35  SKNTVAVVTGANKGIGFALVRRLAQLELTVILTARDQERGFNAVQTLRNEGLP-VLFRRL 94
           +K   AVVTG NKGIGF + ++L+   + V+LT RD  RG  AV+ L+N     V+F +L
Sbjct: 10  TKMRCAVVTGGNKGIGFEICKQLSSSGIMVVLTCRDVTRGLEAVEKLKNSNHENVVFHQL 69

Query: 95  DVSDPL-SIASFAAWFTANFQALDILVNNAAVS------------FNDINENS------- 154
           DV+DP+ +++S A +  A F  LDILVNNA V+             +DI E+S       
Sbjct: 70  DVTDPITTMSSLADFIKARFGKLDILVNNAGVAGFSVDADRFKAMISDIGEDSEEVVKIY 129

Query: 155 ------------VANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTIDK 214
                          AE  +K NYYG K +T+ LLP  + S     RI+N++S  G++  
Sbjct: 130 EKPEAQELMSETYELAEECLKINYYGVKSVTEVLLPLLQLS--DSPRIVNVSSSTGSLKY 189

Query: 215 VKNVKVREILGSED-VGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYT 274
           V N    EILG  D + EE I+ VVN  L D K    +  GWP+    Y  SK  LNAYT
Sbjct: 190 VSNETALEILGDGDALTEERIDMVVNMLLKDFKENLIETNGWPSFGAAYTTSKACLNAYT 249

Query: 275 AVLAKRFAQFGTSVSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHF 302
            VLAK+  +F     VN  CPG  +T M  G G +TA+  A    R+AL P    P+G F
Sbjct: 250 RVLAKKIPKF----QVNCVCPGLVKTEMNYGIGNYTADEGAKHVVRIALFPDDG-PSGFF 303

BLAST of Moc09g41680 vs. ExPASy Swiss-Prot
Match: Q6WAU1 ((-)-isopiperitenone reductase OS=Mentha piperita OX=34256 PE=1 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.3e-38
Identity = 118/305 (38.69%), Postives = 165/305 (54.10%), Query Frame = 0

Query: 40  AVVTGANKGIGFALVRRLAQLELTVILTARDQERGFNAVQTLRNE----GLPVLFRRLDV 99
           A+VTGANKGIGF + R+LA+  + VILT+R+++RG  A Q L  E       ++F +LDV
Sbjct: 8   ALVTGANKGIGFEICRQLAEKGIIVILTSRNEKRGLEARQKLLKELNVSENRLVFHQLDV 67

Query: 100 SDPLSIASFAAWFTANFQALDILVNNAAVS----------FNDINE-------------- 159
           +D  S+A+ A +  + F  LDILVNNA VS          FN+  E              
Sbjct: 68  TDLASVAAVAVFIKSKFGKLDILVNNAGVSGVEMVGDVSVFNEYIEADFKALQALEAGAK 127

Query: 160 -----NSVANAETVMK---------TNYYGAKLLTQALLPAFRSSAPSRARILNITSRLG 219
                   AN E + K         TNYYG K LTQAL+P  + S PS  RI+N++S  G
Sbjct: 128 EEPPFKPKANGEMIEKFEGAKDCVVTNYYGPKRLTQALIPLLQLS-PS-PRIVNVSSSFG 187

Query: 220 TIDKVKNVKVREILGSED-VGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLAL 279
           ++  + N   + +LG ED + EE ++ VV  FL D+K G  ++  WP  +    VSK AL
Sbjct: 188 SLLLLWNEWAKGVLGDEDRLTEERVDEVVEVFLKDIKEGKLEESQWPPHFAAERVSKAAL 247

Query: 280 NAYTAVLAKRFAQFGTSVSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLP 302
           NAYT + AK++  F     +N+ CPG+ +T +T   G  +   AA V  +LALLP    P
Sbjct: 248 NAYTKIAAKKYPSF----RINAICPGYAKTDITFHAGPLSVAEAAQVPVKLALLPDGG-P 305

BLAST of Moc09g41680 vs. ExPASy Swiss-Prot
Match: P48758 (Carbonyl reductase [NADPH] 1 OS=Mus musculus OX=10090 GN=Cbr1 PE=1 SV=3)

HSP 1 Score: 159.1 bits (401), Expect = 8.4e-38
Identity = 97/265 (36.60%), Postives = 159/265 (60.00%), Query Frame = 0

Query: 35  SKNTVAVVTGANKGIGFALVRRLA-QLELTVILTARDQERGFNAVQTLRNEGLPVLFRRL 94
           S   VA+VTGANKGIGFA+ R L  +    V+L ARD+ERG  AVQ L+ EGL   F +L
Sbjct: 3   SSRPVALVTGANKGIGFAITRDLCRKFSGDVVLAARDEERGQTAVQKLQAEGLSPRFHQL 62

Query: 95  DVSDPLSIASFAAWFTANFQALDILVNNAAVSFNDINENSV--ANAETVMKTNYYGAKLL 154
           D+ +P SI +   +    +  LD+LVNNA ++F  +N+++     AE  MKTN++G + +
Sbjct: 63  DIDNPQSIRALRDFLLKEYGGLDVLVNNAGIAFK-VNDDTPFHIQAEVTMKTNFFGTRDV 122

Query: 155 TQALLPAFRSSAPSRARILNITSRLGTIDKVKN--VKVREILGSEDVGEEDIEGVVNAFL 214
            + LLP  +     + R++N++S + ++  +KN  +++++   SE + EE++ G++N F+
Sbjct: 123 CKELLPLIK----PQGRVVNVSSMV-SLRALKNCRLELQQKFRSETITEEELVGLMNKFV 182

Query: 215 GDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRF--AQFGTSVSVNSFCPGFTQTS 274
            D K+G   +EGWP   + Y V+K+ +   + +LA++    + G  + +N+ CPG+ +T 
Sbjct: 183 EDTKKGVHAEEGWPN--SAYGVTKIGVTVLSRILARKLNEQRRGDKILLNACCPGWVRTD 242

Query: 275 MTRGKGTHTAEAAAAVGARLALLPP 293
           M   K T + E  A     LALLPP
Sbjct: 243 MAGPKATKSPEEGAETPVYLALLPP 259

BLAST of Moc09g41680 vs. ExPASy TrEMBL
Match: A0A6J1DC35 ((+)-neomenthol dehydrogenase-like OS=Momordica charantia OX=3673 GN=LOC111019666 PE=3 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 7.1e-173
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0

Query: 1   MPNSRSQISSSMDLDSKEFDLSSPSSSPFSHRWWSKNTVAVVTGANKGIGFALVRRLAQL 60
           MPNSRSQISSSMDLDSKEFDLSSPSSSPFSHRWWSKNTVAVVTGANKGIGFALVRRLAQL
Sbjct: 1   MPNSRSQISSSMDLDSKEFDLSSPSSSPFSHRWWSKNTVAVVTGANKGIGFALVRRLAQL 60

Query: 61  ELTVILTARDQERGFNAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVN 120
           ELTVILTARDQERGFNAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVN
Sbjct: 61  ELTVILTARDQERGFNAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVN 120

Query: 121 NAAVSFNDINENSVANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTID 180
           NAAVSFNDINENSVANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTID
Sbjct: 121 NAAVSFNDINENSVANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTID 180

Query: 181 KVKNVKVREILGSEDVGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYT 240
           KVKNVKVREILGSEDVGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYT
Sbjct: 181 KVKNVKVREILGSEDVGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYT 240

Query: 241 AVLAKRFAQFGTSVSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHF 300
           AVLAKRFAQFGTSVSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHF
Sbjct: 241 AVLAKRFAQFGTSVSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHF 300

Query: 301 FSWGPNYDVPKNSRL 316
           FSWGPNYDVPKNSRL
Sbjct: 301 FSWGPNYDVPKNSRL 315

BLAST of Moc09g41680 vs. ExPASy TrEMBL
Match: A0A6J1HTC2 ((+)-neomenthol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111466403 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.9e-133
Identity = 248/302 (82.12%), Postives = 270/302 (89.40%), Query Frame = 0

Query: 14  LDSKEFDLSSPSSSPFSHRWWSKNTVAVVTGANKGIGFALVRRLAQLELTVILTARDQER 73
           +DSKEFDLSS SSSPFS RWWS NTVAVVTGANKGIGFALVRRLAQ+ELTVILT R++ R
Sbjct: 1   MDSKEFDLSSASSSPFSDRWWSNNTVAVVTGANKGIGFALVRRLAQMELTVILTTRNEGR 60

Query: 74  GFNAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVNNAAVSFNDINENS 133
           G  AV++LRNEGL V FRRLDVSDP SI +FAAWF ANF ALDILVNNAAVSFNDI+ NS
Sbjct: 61  GLKAVESLRNEGLNVRFRRLDVSDPASINAFAAWFVANFPALDILVNNAAVSFNDIDHNS 120

Query: 134 VANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTIDKVKNVKVREILGS 193
           V NAETV+KTN+YGAKLLTQALLP FRSS+ SR+RILNITSRLGTIDKVKN KV+EIL S
Sbjct: 121 VENAETVIKTNFYGAKLLTQALLPLFRSSSSSRSRILNITSRLGTIDKVKNEKVKEILQS 180

Query: 194 EDVGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRFAQFGTS 253
           ED+ EEDI+GVV+AFL DVKRGTWKK+GWPA+WT+YAVSKLALNAYT VLAKR      S
Sbjct: 181 EDLAEEDIDGVVDAFLQDVKRGTWKKKGWPALWTDYAVSKLALNAYTRVLAKR----SRS 240

Query: 254 VSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHFFSWGPNYDVPKNS 313
           VSVNSFCPGFTQTSMT GKGTHTA+AAA VGARLALLPPHRLPTG FFSWGPNYD P+NS
Sbjct: 241 VSVNSFCPGFTQTSMTGGKGTHTADAAAVVGARLALLPPHRLPTGQFFSWGPNYDTPRNS 298

Query: 314 RL 316
           +L
Sbjct: 301 KL 298

BLAST of Moc09g41680 vs. ExPASy TrEMBL
Match: A0A6J1G196 ((+)-neomenthol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111449752 PE=3 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 2.1e-132
Identity = 249/302 (82.45%), Postives = 270/302 (89.40%), Query Frame = 0

Query: 14  LDSKEFDLSSPSSSPFSHRWWSKNTVAVVTGANKGIGFALVRRLAQLELTVILTARDQER 73
           +DSKEFDLSS SSSPFS RWWS NTVAVVTGANKGIGFALVRRLAQ+ELTVILT R++ R
Sbjct: 1   MDSKEFDLSSASSSPFSDRWWSNNTVAVVTGANKGIGFALVRRLAQMELTVILTTRNEGR 60

Query: 74  GFNAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVNNAAVSFNDINENS 133
           G  AV++LRNEGL VLFRRLDVSDP SI +FAAW  ANF ALDILVNNAAVSFNDI+ NS
Sbjct: 61  GLKAVESLRNEGLNVLFRRLDVSDPASINAFAAWVVANFPALDILVNNAAVSFNDIDHNS 120

Query: 134 VANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTIDKVKNVKVREILGS 193
           V NAETV+KTN+YGAKLLTQALLP FRSS+ SR+RILNITSRLGTIDKVKN KV+EIL S
Sbjct: 121 VENAETVIKTNFYGAKLLTQALLPLFRSSS-SRSRILNITSRLGTIDKVKNEKVKEILQS 180

Query: 194 EDVGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRFAQFGTS 253
           ED+ EEDIEGVV+AFL DVKRGTWKK+GWPA+WT+YAVSKLALNAYT VLAKR      S
Sbjct: 181 EDLAEEDIEGVVDAFLQDVKRGTWKKKGWPALWTDYAVSKLALNAYTRVLAKR----SRS 240

Query: 254 VSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHFFSWGPNYDVPKNS 313
           VSVNSFCPGFTQTSMT GKGTHTA+AAA VGARLALLPPHRLPTG FFSWGPNYD P+NS
Sbjct: 241 VSVNSFCPGFTQTSMTGGKGTHTADAAAVVGARLALLPPHRLPTGQFFSWGPNYDTPRNS 297

Query: 314 RL 316
           +L
Sbjct: 301 KL 297

BLAST of Moc09g41680 vs. ExPASy TrEMBL
Match: A0A6J1KA32 ((+)-neomenthol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111493032 PE=3 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 1.8e-131
Identity = 246/302 (81.46%), Postives = 273/302 (90.40%), Query Frame = 0

Query: 14  LDSKEFDLSSPSSSPFSHRWWSKNTVAVVTGANKGIGFALVRRLAQLELTVILTARDQER 73
           ++SKEFDLSS SSSP S RWWSKNTVAVVTGANKGIGFALVRRLAQ ELTVILTARD+ R
Sbjct: 1   MESKEFDLSS-SSSPVSDRWWSKNTVAVVTGANKGIGFALVRRLAQSELTVILTARDEAR 60

Query: 74  GFNAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVNNAAVSFNDINENS 133
           G  AV+TLRNEGL VLFRRLDVSDP+SI++FAAWFTANFQALDILVNNAAVSFNDIN+NS
Sbjct: 61  GLQAVETLRNEGLHVLFRRLDVSDPVSISAFAAWFTANFQALDILVNNAAVSFNDINQNS 120

Query: 134 VANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTIDKVKNVKVREILGS 193
           V +AETV+KTN+YGAKLLT+ALLP F SS+PSR+RILNITSRLGTIDKV+N+KV+EIL S
Sbjct: 121 VEHAETVLKTNFYGAKLLTEALLPYF-SSSPSRSRILNITSRLGTIDKVRNIKVKEILQS 180

Query: 194 EDVGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRFAQFGTS 253
           +D+ EEDI G+V AFL DV+RGTW  +GWPAVWT+YAVSKLALN+YT VLA R+  FG S
Sbjct: 181 KDLAEEDIHGIVCAFLEDVQRGTWTNQGWPAVWTDYAVSKLALNSYTRVLANRYGAFG-S 240

Query: 254 VSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHFFSWGPNYDVPKNS 313
           VSVN FCPGFTQTSMT GKGTHTA+AAA VGARLALLPPHRLPTG FFSWGPNY  PKNS
Sbjct: 241 VSVNCFCPGFTQTSMTGGKGTHTADAAALVGARLALLPPHRLPTGQFFSWGPNYVTPKNS 299

Query: 314 RL 316
           +L
Sbjct: 301 KL 299

BLAST of Moc09g41680 vs. ExPASy TrEMBL
Match: A0A6J1GZE8 ((+)-neomenthol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111458888 PE=3 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 2.3e-131
Identity = 245/302 (81.13%), Postives = 271/302 (89.74%), Query Frame = 0

Query: 14  LDSKEFDLSSPSSSPFSHRWWSKNTVAVVTGANKGIGFALVRRLAQLELTVILTARDQER 73
           ++SKEFDLSS  SSP S RWWSKNTVAVVTGANKGIGFALVRRLAQ ELTVILTARD+ R
Sbjct: 1   MESKEFDLSSSLSSPVSDRWWSKNTVAVVTGANKGIGFALVRRLAQSELTVILTARDEAR 60

Query: 74  GFNAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVNNAAVSFNDINENS 133
           G  AV+TLRNEGL VLFRRLDVSDP+SI++FAAWFTANFQALDILVNNAAVSFN IN+NS
Sbjct: 61  GLQAVETLRNEGLHVLFRRLDVSDPVSISAFAAWFTANFQALDILVNNAAVSFNQINQNS 120

Query: 134 VANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTIDKVKNVKVREILGS 193
           V +AETV+KTN+YGAKLLT+ALLP FRSS PSR+RILNITSRLGTID+V+N+KV+EIL S
Sbjct: 121 VEHAETVLKTNFYGAKLLTEALLPYFRSS-PSRSRILNITSRLGTIDRVRNMKVKEILQS 180

Query: 194 EDVGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRFAQFGTS 253
           +D+ EEDI GVV AFL DV+RGTW  +GWPAVWT+YAVSKLALN+YT VLA R+  FG S
Sbjct: 181 KDLAEEDIHGVVCAFLEDVQRGTWTNQGWPAVWTDYAVSKLALNSYTKVLANRYGAFG-S 240

Query: 254 VSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHFFSWGPNYDVPKNS 313
           VSVN FCPGFTQTSMT GKGTHTA+AAA VGARLALLPPHRLPTG FFSWGPNY  PKNS
Sbjct: 241 VSVNCFCPGFTQTSMTGGKGTHTADAAALVGARLALLPPHRLPTGQFFSWGPNYVTPKNS 300

Query: 314 RL 316
           +L
Sbjct: 301 KL 300

BLAST of Moc09g41680 vs. TAIR 10
Match: AT3G59710.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 314.3 bits (804), Expect = 1.1e-85
Identity = 162/272 (59.56%), Postives = 206/272 (75.74%), Query Frame = 0

Query: 31  HRWWSKNTVAVVTGANKGIGFALVRRLAQLELTVILTARDQERGFNAVQTLRNEGL-PVL 90
           H WWS+ T AVVTGANKGIGFA+V+RL +L LTV+LTAR+ E G  A ++LR  G   V 
Sbjct: 22  HEWWSEETTAVVTGANKGIGFAVVKRLLELGLTVVLTARNAENGSQAAESLRRIGFGNVH 81

Query: 91  FRRLDVSDPLSIASFAAWFTANFQALDILVNNAAVSFNDINENSVANAETVMKTNYYGAK 150
           F  LD+SDP SIA+FA+WF  N   LDILVNNAAVSFN + EN +   ET++KTN+YGAK
Sbjct: 82  FCCLDISDPSSIAAFASWFGRNLGILDILVNNAAVSFNAVGENLIKEPETIIKTNFYGAK 141

Query: 151 LLTQALLPAFRSSAPSRARILNITSRLGTIDKVKNVKVREILGSEDVGEEDIEGVVNAFL 210
           LLT+ALLP FR S  S +RILN++SRLGT++K+++  +R IL SED+  E I+  +  FL
Sbjct: 142 LLTEALLPLFRRSV-SVSRILNMSSRLGTLNKLRSPSIRRILESEDLTNEQIDATLTQFL 201

Query: 211 GDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRFAQFGTSVSVNSFCPGFTQTSMT 270
            DVK GTW+K+GWP  W +YA+SKLALNAY+ VLA+R+   G  +SVN  CPGFT+TSMT
Sbjct: 202 QDVKSGTWEKQGWPENWPDYAISKLALNAYSRVLARRYD--GKKLSVNCLCPGFTRTSMT 261

Query: 271 RGKGTHTAEAAAAVGARLALLPPHRLPTGHFF 302
            G+GTHTA+ AAA+ A+L LLPP +L TG F+
Sbjct: 262 GGQGTHTADEAAAIVAKLVLLPPEKLATGKFY 290

BLAST of Moc09g41680 vs. TAIR 10
Match: AT5G61830.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 233.0 bits (593), Expect = 3.2e-61
Identity = 135/294 (45.92%), Postives = 191/294 (64.97%), Query Frame = 0

Query: 20  DLSSPSSSPFS--HRWWSKNTVAVVTGANKGIGFALVRRLAQLELTVILTARDQERGFNA 79
           ++S   + P+S  HRWWS   VAVVTG+N+GIGF + R+LA   LTV+LTAR+   G  A
Sbjct: 17  EISLLRTIPYSDHHRWWSCENVAVVTGSNRGIGFEIARQLAVHGLTVVLTARNVNAGLEA 76

Query: 80  VQTLRN--EGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVNNAAVSFNDINENSVA 139
           V++LR+  EGL V F +LDV+D  SI  F  W    F  LDILVNNA V++N  ++N+V 
Sbjct: 77  VKSLRHQEEGLKVYFHQLDVTDSSSIREFGCWLKQTFGGLDILVNNAGVNYNLGSDNTVE 136

Query: 140 NAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTI----DKVKNVKVREIL 199
            AETV+ TNY G K +T+A++P  R S P  AR++N++SRLG +    +++ NV++R+ L
Sbjct: 137 FAETVISTNYQGTKNMTKAMIPLMRPS-PHGARVVNVSSRLGRVNGRRNRLANVELRDQL 196

Query: 200 GSED-VGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRFAQF 259
            S D + EE I+  V+ F+  VK GTW+  GWP  +T+Y++SKLA+NAYT ++AK   + 
Sbjct: 197 SSPDLLTEELIDRTVSKFINQVKDGTWESGGWPQTFTDYSMSKLAVNAYTRLMAKELERR 256

Query: 260 G--TSVSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHFFS 303
           G    + VNSFCPG+ +T+MT   G    E AA  G  L+L+      TG FF+
Sbjct: 257 GEEEKIYVNSFCPGWVKTAMTGYAGNMPPEDAADTGVWLSLVLSEEAVTGKFFA 309

BLAST of Moc09g41680 vs. TAIR 10
Match: AT5G51030.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 219.9 bits (559), Expect = 2.8e-57
Identity = 130/306 (42.48%), Postives = 188/306 (61.44%), Query Frame = 0

Query: 6   SQISSSMDLDSKEFDLSSPSSSPFS--HRWWSKNTVAVVTGANKGIGFALVRRLAQLELT 65
           S+  S    D +  ++S   + P+S   RWW+  TVAVVTGAN+GIGF +VR+LA   LT
Sbjct: 4   SKEKSKEKRDKRLQEISLLRTIPYSDHQRWWTSETVAVVTGANRGIGFEMVRQLAGHGLT 63

Query: 66  VILTARDQERGFNAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVNNAA 125
           VILT+RD+  G  A + L+  G  V F RLD+ D  SI  F  W    +  +D+L+NNA 
Sbjct: 64  VILTSRDENVGVEAAKILQEGGFNVDFHRLDILDSSSIQEFCEWIKEKYGFIDVLINNAG 123

Query: 126 VSFNDINENSVANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTI---- 185
           V++N  ++NSV  +  V+ TNYYG K +  A++P  R +    ARI+N+TSRLG +    
Sbjct: 124 VNYNVGSDNSVEFSHMVISTNYYGTKNIINAMIPLMRHACQG-ARIVNVTSRLGRLKGRH 183

Query: 186 DKVKNVKVR-EILGSEDVGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNA 245
            K++N  VR +++  + + EE ++  V+ FL  V+ GTW+  GWP  +T+Y+VSK+A+NA
Sbjct: 184 SKLENEDVRAKLMDVDSLTEEIVDKTVSEFLKQVEEGTWESGGWPHSFTDYSVSKMAVNA 243

Query: 246 YTAVLAKRFAQ--FGTSVSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLP 303
           YT VLAK  ++   G  +  N FCPG+ +T+MT   G  +AE  A  G  LALLP   + 
Sbjct: 244 YTRVLAKELSERPEGEKIYANCFCPGWVKTAMTGYAGNVSAEDGADTGVWLALLPDQAI- 303

BLAST of Moc09g41680 vs. TAIR 10
Match: AT3G61220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 181.8 bits (460), Expect = 8.5e-46
Identity = 114/287 (39.72%), Postives = 159/287 (55.40%), Query Frame = 0

Query: 40  AVVTGANKGIGFALVRRLAQLELTVILTARDQERGFNAVQTLRNE----GLPVLFRRLDV 99
           AVVTGAN+GIGF + R+LA   + V+LT+RD+ RG  AV+TL+ E       +LF +LDV
Sbjct: 9   AVVTGANRGIGFEICRQLASEGIRVVLTSRDENRGLEAVETLKKELEISDQSLLFHQLDV 68

Query: 100 SDPLSIASFAAWFTANFQALDILVNNAAV-------------------SFNDINENSVAN 159
           +DP SI S A +    F  LDILVNNA +                    +++I   +   
Sbjct: 69  ADPASITSLAEFVKTQFGKLDILVNNAGIGGIITDAEALRAGAGKEGFKWDEIITETYEL 128

Query: 160 AETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTIDKVKNVKVREILG-SED 219
            E  +K NYYG K + +A +P  + S     RI+N++S +G +  V N   + IL  +E+
Sbjct: 129 TEECIKINYYGPKRMCEAFIPLLKLS--DSPRIVNVSSSMGQLKNVLNEWAKGILSDAEN 188

Query: 220 VGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRFAQFGTSVS 279
           + EE I+ V+N  L D K GT K++ W    + Y VSK +LN YT VLAK+  +F     
Sbjct: 189 LTEERIDQVINQLLNDFKEGTVKEKNWAKFMSAYVVSKASLNGYTRVLAKKHPEF----R 248

Query: 280 VNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHFFS 303
           VN+ CPGF +T M    G  + E  A+   RLALLP    P+G FFS
Sbjct: 249 VNAVCPGFVKTDMNFKTGVLSVEEGASSPVRLALLPHQETPSGCFFS 289

BLAST of Moc09g41680 vs. TAIR 10
Match: AT3G61220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 181.8 bits (460), Expect = 8.5e-46
Identity = 114/287 (39.72%), Postives = 159/287 (55.40%), Query Frame = 0

Query: 40  AVVTGANKGIGFALVRRLAQLELTVILTARDQERGFNAVQTLRNE----GLPVLFRRLDV 99
           AVVTGAN+GIGF + R+LA   + V+LT+RD+ RG  AV+TL+ E       +LF +LDV
Sbjct: 16  AVVTGANRGIGFEICRQLASEGIRVVLTSRDENRGLEAVETLKKELEISDQSLLFHQLDV 75

Query: 100 SDPLSIASFAAWFTANFQALDILVNNAAV-------------------SFNDINENSVAN 159
           +DP SI S A +    F  LDILVNNA +                    +++I   +   
Sbjct: 76  ADPASITSLAEFVKTQFGKLDILVNNAGIGGIITDAEALRAGAGKEGFKWDEIITETYEL 135

Query: 160 AETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTIDKVKNVKVREILG-SED 219
            E  +K NYYG K + +A +P  + S     RI+N++S +G +  V N   + IL  +E+
Sbjct: 136 TEECIKINYYGPKRMCEAFIPLLKLS--DSPRIVNVSSSMGQLKNVLNEWAKGILSDAEN 195

Query: 220 VGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRFAQFGTSVS 279
           + EE I+ V+N  L D K GT K++ W    + Y VSK +LN YT VLAK+  +F     
Sbjct: 196 LTEERIDQVINQLLNDFKEGTVKEKNWAKFMSAYVVSKASLNGYTRVLAKKHPEF----R 255

Query: 280 VNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHFFS 303
           VN+ CPGF +T M    G  + E  A+   RLALLP    P+G FFS
Sbjct: 256 VNAVCPGFVKTDMNFKTGVLSVEEGASSPVRLALLPHQETPSGCFFS 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151760.11.5e-172100.00(+)-neomenthol dehydrogenase-like [Momordica charantia][more]
XP_038891404.13.5e-13482.67(+)-neomenthol dehydrogenase-like [Benincasa hispida][more]
XP_023542237.11.3e-13382.45(+)-neomenthol dehydrogenase-like [Cucurbita pepo subsp. pepo][more]
XP_022966808.13.9e-13382.12(+)-neomenthol dehydrogenase-like [Cucurbita maxima][more]
KAG6573598.14.3e-13282.12(+)-neomenthol dehydrogenase, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
Match NameE-valueIdentityDescription
Q9M2E21.2e-4439.72(+)-neomenthol dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=SDR1 PE=1 SV=1[more]
Q9ZUH54.3e-4238.46Short-chain dehydrogenase/reductase 2b OS=Arabidopsis thaliana OX=3702 GN=SDR2b ... [more]
A4UHT71.4e-4038.87Salutaridine reductase OS=Papaver bracteatum OX=215227 GN=SALR PE=1 SV=1[more]
Q6WAU11.3e-3838.69(-)-isopiperitenone reductase OS=Mentha piperita OX=34256 PE=1 SV=1[more]
P487588.4e-3836.60Carbonyl reductase [NADPH] 1 OS=Mus musculus OX=10090 GN=Cbr1 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1DC357.1e-173100.00(+)-neomenthol dehydrogenase-like OS=Momordica charantia OX=3673 GN=LOC111019666... [more]
A0A6J1HTC21.9e-13382.12(+)-neomenthol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111466403 PE... [more]
A0A6J1G1962.1e-13282.45(+)-neomenthol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111449752 ... [more]
A0A6J1KA321.8e-13181.46(+)-neomenthol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111493032 PE... [more]
A0A6J1GZE82.3e-13181.13(+)-neomenthol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111458888 ... [more]
Match NameE-valueIdentityDescription
AT3G59710.11.1e-8559.56NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G61830.13.2e-6145.92NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G51030.12.8e-5742.48NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G61220.18.5e-4639.72NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G61220.28.5e-4639.72NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 229..248
score: 31.8
coord: 113..124
score: 49.24
coord: 252..269
score: 33.59
coord: 39..56
score: 49.75
coord: 161..177
score: 24.43
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 229..248
score: 32.68
coord: 113..124
score: 45.96
coord: 167..175
score: 41.14
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 224..272
e-value: 1.1E-6
score: 28.3
coord: 39..178
e-value: 5.9E-28
score: 97.7
NoneNo IPR availableGENE3D3.40.50.720coord: 32..307
e-value: 6.3E-73
score: 247.3
NoneNo IPR availablePANTHERPTHR43490:SF60NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEINcoord: 33..306
NoneNo IPR availablePANTHERPTHR43490(+)-NEOMENTHOL DEHYDROGENASEcoord: 33..306
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 39..303

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g41680.1Moc09g41680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity