Moc09g41000 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc09g41000
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptioncytochrome P450 71B37-like
Locationchr9: 31265622 .. 31267354 (-)
RNA-Seq ExpressionMoc09g41000
SyntenyMoc09g41000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTAACTTAGATCAGATGATGCATAGCCTTTCCATCTGGGTCTCTCTGCTCTTGCTGTTGGCTTCCCTACTGTTGGGTCACTTGCACCTCATCGGCTCCATCCCCCATCGCTCTTTGTGGAAACTTTCAAGAAAATATGGCTCCGTAATGCTCCTCAAACTGGGCTCTATTCCAACCATCGTAATCTCCTCTGCTGCTGCTGCAAGAGAGGTGTTACAAGTGCATGATCTTGCTTGTTGCAGCCGGCCTCGCTTAGCCGCCAGTGCAAGATTTTCATACAACTTTTTAGACATAGGTTTATCGCCATATAGTGATCATTGGAGGGAAGTTCGAAAGATTAGTGTGCTTGAGCTTTTCAGTGCTAGGCGGGTACAGTCGTTTCAGCTTATTAGAGAAGAAGAAGTCGGTTTGCTGTTAAAGTCCCTCTTTGAATCGTCATCTTCTGCTGCTCCAGTTGATCTGAGCGAGAAATCTTATACTCTCACTGCAAACATAATGACCCGGATTGCATTTGGCAAGAGCTTTAGAGGGGGTGAGCTAGATAATGGAAATTTTCACGAGGTTATCCGTAGAGCAATAGCAGCATTGGCAAGCTTCTCAGTGACTGACTTCTTTCCCACAGTGGGTTGGATTATTGATCGGGTCAGTGGTGTTCATGGGAGGTTGGAGAAGAGCTTTGCTGAGCTGGATGCTTTTCTCCAACATGTAGTTGATGATCGCATCAACTTCATGGCGACTTCTGAAAATGAAAACATTATTGATGTTTTGTTGAGAATGGAGAGAGAGCGCTCTGAATCTGATGCTCTGCAACTCACTAGAGATTGCATCAAGGCAATTATCATGGTAAGCTCAAGATAATAAGGCAGACATAGCTTCAAACCGATTCTGATCATCATTATGGTTTTTTATTTTTCTATTTTCTGTTTTTGTTTTTTAGACCCTTGATCATCATTTTGGTTACCGAATCCCCTGTTCTTCAAAAAAGAAAGTCCAATAACTAAATATAAGAAAATTAAATGGTTATTAAAGAGCACCTCTTGTTTTAGTTTTCCAACTAATATTGAAGCTGAGGATGTGTGCGGTTTAATTAGGATATATTTATAGCTGGAGTGAACCCAGGAGCGGGCACCATAATTTGGGCAATGGCAGAGCTGATTAGGAATCCACGGGTAATGAAGAAGCTACAATATGAGATTAGAAGTTGCATAAAAGAAGATCAAGTGAAGGAGAGCGACCTGGAAAAGCTTCAATATCTAAGAATGGTAGTGAAGGAGGTTTTGAGGTTGCATGCGCCTGCCCCACTTCTCCTTCCAAGAGAAACCATGTCTCATTTCAAGCTCAATGGTTACGATATTGCTCCAATGGTTCATCTTCATGTCAACGTTTGGGCGATCGGACGAGACCCGGATTCTTGGAGTAACCCAGAAGAGTTCTTGCCAGAGAGATTTATGGAAAGCTGTGTGGATTTCAGAGGGCAGAATTTTGAGTTATTACCGTTTGGAGCTGGTCGGAGGAGCTGTCCTGGTGTGAACATGGGGGCCATTACGGTGGAGTTGATATTGGCTAATCTGTTACTTTGTTTTGATTGGAAATTGCCTGATGGAATGAAAGAAGATGTGGACATGGAAGAGGATGCTGGTCTAACAGTTGCAAAGAAATCACCTCTTAAACTTGTTCCAGTCTGCTACGGATTTGGCTCCACTCATGATCATGAATGCCAAGTACCATGA

mRNA sequence

ATGTTTAACTTAGATCAGATGATGCATAGCCTTTCCATCTGGGTCTCTCTGCTCTTGCTGTTGGCTTCCCTACTGTTGGGTCACTTGCACCTCATCGGCTCCATCCCCCATCGCTCTTTGTGGAAACTTTCAAGAAAATATGGCTCCGTAATGCTCCTCAAACTGGGCTCTATTCCAACCATCGTAATCTCCTCTGCTGCTGCTGCAAGAGAGGTGTTACAAGTGCATGATCTTGCTTGTTGCAGCCGGCCTCGCTTAGCCGCCAGTGCAAGATTTTCATACAACTTTTTAGACATAGGTTTATCGCCATATAGTGATCATTGGAGGGAAGTTCGAAAGATTAGTGTGCTTGAGCTTTTCAGTGCTAGGCGGGTACAGTCGTTTCAGCTTATTAGAGAAGAAGAAGTCGGTTTGCTGTTAAAGTCCCTCTTTGAATCGTCATCTTCTGCTGCTCCAGTTGATCTGAGCGAGAAATCTTATACTCTCACTGCAAACATAATGACCCGGATTGCATTTGGCAAGAGCTTTAGAGGGGGTGAGCTAGATAATGGAAATTTTCACGAGGTTATCCGTAGAGCAATAGCAGCATTGGCAAGCTTCTCAGTGACTGACTTCTTTCCCACAGTGGGTTGGATTATTGATCGGGTCAGTGGTGTTCATGGGAGGTTGGAGAAGAGCTTTGCTGAGCTGGATGCTTTTCTCCAACATGTAGTTGATGATCGCATCAACTTCATGGCGACTTCTGAAAATGAAAACATTATTGATGTTTTGTTGAGAATGGAGAGAGAGCGCTCTGAATCTGATGCTCTGCAACTCACTAGAGATTGCATCAAGGCAATTATCATGGATATATTTATAGCTGGAGTGAACCCAGGAGCGGGCACCATAATTTGGGCAATGGCAGAGCTGATTAGGAATCCACGGGTAATGAAGAAGCTACAATATGAGATTAGAAGTTGCATAAAAGAAGATCAAGTGAAGGAGAGCGACCTGGAAAAGCTTCAATATCTAAGAATGGTAGTGAAGGAGGTTTTGAGGTTGCATGCGCCTGCCCCACTTCTCCTTCCAAGAGAAACCATGTCTCATTTCAAGCTCAATGGTTACGATATTGCTCCAATGGTTCATCTTCATGTCAACGTTTGGGCGATCGGACGAGACCCGGATTCTTGGAGTAACCCAGAAGAGTTCTTGCCAGAGAGATTTATGGAAAGCTGTGTGGATTTCAGAGGGCAGAATTTTGAGTTATTACCGTTTGGAGCTGGTCGGAGGAGCTGTCCTGGTGTGAACATGGGGGCCATTACGGTGGAGTTGATATTGGCTAATCTGTTACTTTGTTTTGATTGGAAATTGCCTGATGGAATGAAAGAAGATGTGGACATGGAAGAGGATGCTGGTCTAACAGTTGCAAAGAAATCACCTCTTAAACTTGTTCCAGTCTGCTACGGATTTGGCTCCACTCATGATCATGAATGCCAAGTACCATGA

Coding sequence (CDS)

ATGTTTAACTTAGATCAGATGATGCATAGCCTTTCCATCTGGGTCTCTCTGCTCTTGCTGTTGGCTTCCCTACTGTTGGGTCACTTGCACCTCATCGGCTCCATCCCCCATCGCTCTTTGTGGAAACTTTCAAGAAAATATGGCTCCGTAATGCTCCTCAAACTGGGCTCTATTCCAACCATCGTAATCTCCTCTGCTGCTGCTGCAAGAGAGGTGTTACAAGTGCATGATCTTGCTTGTTGCAGCCGGCCTCGCTTAGCCGCCAGTGCAAGATTTTCATACAACTTTTTAGACATAGGTTTATCGCCATATAGTGATCATTGGAGGGAAGTTCGAAAGATTAGTGTGCTTGAGCTTTTCAGTGCTAGGCGGGTACAGTCGTTTCAGCTTATTAGAGAAGAAGAAGTCGGTTTGCTGTTAAAGTCCCTCTTTGAATCGTCATCTTCTGCTGCTCCAGTTGATCTGAGCGAGAAATCTTATACTCTCACTGCAAACATAATGACCCGGATTGCATTTGGCAAGAGCTTTAGAGGGGGTGAGCTAGATAATGGAAATTTTCACGAGGTTATCCGTAGAGCAATAGCAGCATTGGCAAGCTTCTCAGTGACTGACTTCTTTCCCACAGTGGGTTGGATTATTGATCGGGTCAGTGGTGTTCATGGGAGGTTGGAGAAGAGCTTTGCTGAGCTGGATGCTTTTCTCCAACATGTAGTTGATGATCGCATCAACTTCATGGCGACTTCTGAAAATGAAAACATTATTGATGTTTTGTTGAGAATGGAGAGAGAGCGCTCTGAATCTGATGCTCTGCAACTCACTAGAGATTGCATCAAGGCAATTATCATGGATATATTTATAGCTGGAGTGAACCCAGGAGCGGGCACCATAATTTGGGCAATGGCAGAGCTGATTAGGAATCCACGGGTAATGAAGAAGCTACAATATGAGATTAGAAGTTGCATAAAAGAAGATCAAGTGAAGGAGAGCGACCTGGAAAAGCTTCAATATCTAAGAATGGTAGTGAAGGAGGTTTTGAGGTTGCATGCGCCTGCCCCACTTCTCCTTCCAAGAGAAACCATGTCTCATTTCAAGCTCAATGGTTACGATATTGCTCCAATGGTTCATCTTCATGTCAACGTTTGGGCGATCGGACGAGACCCGGATTCTTGGAGTAACCCAGAAGAGTTCTTGCCAGAGAGATTTATGGAAAGCTGTGTGGATTTCAGAGGGCAGAATTTTGAGTTATTACCGTTTGGAGCTGGTCGGAGGAGCTGTCCTGGTGTGAACATGGGGGCCATTACGGTGGAGTTGATATTGGCTAATCTGTTACTTTGTTTTGATTGGAAATTGCCTGATGGAATGAAAGAAGATGTGGACATGGAAGAGGATGCTGGTCTAACAGTTGCAAAGAAATCACCTCTTAAACTTGTTCCAGTCTGCTACGGATTTGGCTCCACTCATGATCATGAATGCCAAGTACCATGA

Protein sequence

MFNLDQMMHSLSIWVSLLLLLASLLLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQSFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNFHEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMATSENENIIDVLLRMERERSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCPGVNMGAITVELILANLLLCFDWKLPDGMKEDVDMEEDAGLTVAKKSPLKLVPVCYGFGSTHDHECQVP
Homology
BLAST of Moc09g41000 vs. NCBI nr
Match: XP_022151972.1 (cytochrome P450 71B34-like isoform X1 [Momordica charantia] >XP_022151976.1 cytochrome P450 71B34-like isoform X1 [Momordica charantia])

HSP 1 Score: 981.9 bits (2537), Expect = 2.1e-282
Identity = 494/494 (100.00%), Postives = 494/494 (100.00%), Query Frame = 0

Query: 1   MFNLDQMMHSLSIWVSLLLLLASLLLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPT 60
           MFNLDQMMHSLSIWVSLLLLLASLLLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPT
Sbjct: 1   MFNLDQMMHSLSIWVSLLLLLASLLLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPT 60

Query: 61  IVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIGLSPYSDHWREVRKISVLELF 120
           IVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIGLSPYSDHWREVRKISVLELF
Sbjct: 61  IVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIGLSPYSDHWREVRKISVLELF 120

Query: 121 SARRVQSFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGE 180
           SARRVQSFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGE
Sbjct: 121 SARRVQSFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGE 180

Query: 181 LDNGNFHEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDD 240
           LDNGNFHEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDD
Sbjct: 181 LDNGNFHEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDD 240

Query: 241 RINFMATSENENIIDVLLRMERERSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAM 300
           RINFMATSENENIIDVLLRMERERSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAM
Sbjct: 241 RINFMATSENENIIDVLLRMERERSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAM 300

Query: 301 AELIRNPRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETM 360
           AELIRNPRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETM
Sbjct: 301 AELIRNPRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETM 360

Query: 361 SHFKLNGYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGA 420
           SHFKLNGYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGA
Sbjct: 361 SHFKLNGYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGA 420

Query: 421 GRRSCPGVNMGAITVELILANLLLCFDWKLPDGMKEDVDMEEDAGLTVAKKSPLKLVPVC 480
           GRRSCPGVNMGAITVELILANLLLCFDWKLPDGMKEDVDMEEDAGLTVAKKSPLKLVPVC
Sbjct: 421 GRRSCPGVNMGAITVELILANLLLCFDWKLPDGMKEDVDMEEDAGLTVAKKSPLKLVPVC 480

Query: 481 YGFGSTHDHECQVP 495
           YGFGSTHDHECQVP
Sbjct: 481 YGFGSTHDHECQVP 494

BLAST of Moc09g41000 vs. NCBI nr
Match: XP_022151974.1 (cytochrome P450 71B34-like isoform X2 [Momordica charantia] >XP_022151975.1 cytochrome P450 71B34-like isoform X2 [Momordica charantia])

HSP 1 Score: 969.9 bits (2506), Expect = 8.1e-279
Identity = 488/488 (100.00%), Postives = 488/488 (100.00%), Query Frame = 0

Query: 7   MMHSLSIWVSLLLLLASLLLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSA 66
           MMHSLSIWVSLLLLLASLLLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSA
Sbjct: 1   MMHSLSIWVSLLLLLASLLLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSA 60

Query: 67  AAAREVLQVHDLACCSRPRLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQ 126
           AAAREVLQVHDLACCSRPRLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQ
Sbjct: 61  AAAREVLQVHDLACCSRPRLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQ 120

Query: 127 SFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNF 186
           SFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNF
Sbjct: 121 SFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNF 180

Query: 187 HEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMA 246
           HEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMA
Sbjct: 181 HEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMA 240

Query: 247 TSENENIIDVLLRMERERSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRN 306
           TSENENIIDVLLRMERERSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRN
Sbjct: 241 TSENENIIDVLLRMERERSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRN 300

Query: 307 PRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLN 366
           PRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLN
Sbjct: 301 PRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLN 360

Query: 367 GYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCP 426
           GYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCP
Sbjct: 361 GYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCP 420

Query: 427 GVNMGAITVELILANLLLCFDWKLPDGMKEDVDMEEDAGLTVAKKSPLKLVPVCYGFGST 486
           GVNMGAITVELILANLLLCFDWKLPDGMKEDVDMEEDAGLTVAKKSPLKLVPVCYGFGST
Sbjct: 421 GVNMGAITVELILANLLLCFDWKLPDGMKEDVDMEEDAGLTVAKKSPLKLVPVCYGFGST 480

Query: 487 HDHECQVP 495
           HDHECQVP
Sbjct: 481 HDHECQVP 488

BLAST of Moc09g41000 vs. NCBI nr
Match: XP_023541440.1 (cytochrome P450 71B26-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 776.2 bits (2003), Expect = 1.7e-220
Identity = 401/502 (79.88%), Postives = 439/502 (87.45%), Query Frame = 0

Query: 1   MFNLDQMMHSLSIW-VSLLLLLASL-------------------LLGHLHLIGSIPHRSL 60
           M +LD+MMHSLSIW VSLLLLL+ L                   LLGHLHLIGS+PHRSL
Sbjct: 1   MLDLDRMMHSLSIWVVSLLLLLSFLLLKTKTHNKKLPPGPPKLPLLGHLHLIGSLPHRSL 60

Query: 61  WKLSRKYGSVMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIG 120
           WKLSR YG +MLLKLGSIPTIVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIG
Sbjct: 61  WKLSRTYGPIMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIG 120

Query: 121 LSPYSDHWREVRKISVLELFSARRVQSFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSY 180
           LSPYSDHWREVRKI VLELFSARRVQSF+ IREEEV LLLKS+ +SSS   PVDLSE SY
Sbjct: 121 LSPYSDHWREVRKICVLELFSARRVQSFKSIREEEVALLLKSISQSSSLEIPVDLSEASY 180

Query: 181 TLTANIMTRIAFGKSFRGGELDNGNFHEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVH 240
           T TANI TRIAFGKSFRG  LDNGNFH+VIRRAIAAL SFS+TDFFP VGW+IDR+SGV 
Sbjct: 181 TFTANITTRIAFGKSFRGSGLDNGNFHQVIRRAIAALGSFSMTDFFPKVGWVIDRLSGVS 240

Query: 241 GRLEKSFAELDAFLQHVVDDRINFMATSEN-ENIIDVLLRMERERSESDALQLTRDCIKA 300
           GRLEKSFAELDAFLQ VVDD INF ATS+N ENI+DVLL+MER+ S+ +ALQLTRDCIKA
Sbjct: 241 GRLEKSFAELDAFLQQVVDDHINFKATSQNEENIVDVLLQMERDCSKPNALQLTRDCIKA 300

Query: 301 IIMDIFIAGVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRM 360
           +IMDIFIAGVNPGAGTIIWAM EL+RNPRVMKKLQ EIR+ IKE+QVKE+DLEKLQYL+M
Sbjct: 301 LIMDIFIAGVNPGAGTIIWAMTELVRNPRVMKKLQDEIRTSIKENQVKENDLEKLQYLKM 360

Query: 361 VVKEVLRLHAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPE 420
           V+KEVLRLHAPAPLLLPRET+SHFKLNGYDI P  HL+VNVWAIGRD D W+NPEEFLPE
Sbjct: 361 VMKEVLRLHAPAPLLLPRETLSHFKLNGYDIDPKAHLYVNVWAIGRDLDYWTNPEEFLPE 420

Query: 421 RFMESCVDFRGQNFELLPFGAGRRSCPGVNMGAITVELILANLLLCFDWKLPDGMKEDVD 480
           RF+ S +D++GQNFE LPFGAGRR CPG+NMG +T+EL LANLLL FDWKLP+G K DVD
Sbjct: 421 RFIGSTIDYKGQNFEYLPFGAGRRICPGMNMGIVTMELALANLLLLFDWKLPNGTK-DVD 480

Query: 481 MEEDAGLTVAKKSPLKLVPVCY 482
           MEEDAGLTV+KKSPLKL+PV Y
Sbjct: 481 MEEDAGLTVSKKSPLKLLPVGY 501

BLAST of Moc09g41000 vs. NCBI nr
Match: XP_022945237.1 (cytochrome P450 71B37-like [Cucurbita moschata] >XP_022945238.1 cytochrome P450 71B37-like [Cucurbita moschata])

HSP 1 Score: 769.6 bits (1986), Expect = 1.6e-218
Identity = 398/502 (79.28%), Postives = 435/502 (86.65%), Query Frame = 0

Query: 1   MFNLDQMMHSLSIW-VSLLLLLASL-------------------LLGHLHLIGSIPHRSL 60
           M  LD+MMHSLSIW VSLLLLL  L                   LLGHLHLIGS+PHRSL
Sbjct: 1   MLGLDRMMHSLSIWVVSLLLLLFFLLLKTKTHNKKLPPGPPKLPLLGHLHLIGSLPHRSL 60

Query: 61  WKLSRKYGSVMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIG 120
           WKLSR YG +MLLKLGSIPTIVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIG
Sbjct: 61  WKLSRTYGPIMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIG 120

Query: 121 LSPYSDHWREVRKISVLELFSARRVQSFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSY 180
           LSPYSDHWREVRKI VLELFSARRVQSF+ IREEEV LLLKS+ +SSS   PVDLSE+SY
Sbjct: 121 LSPYSDHWREVRKICVLELFSARRVQSFKSIREEEVALLLKSISQSSSLEIPVDLSEESY 180

Query: 181 TLTANIMTRIAFGKSFRGGELDNGNFHEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVH 240
           T TANI TRIAFGKSFRG  LDNGNFH+VIRRAIAAL S S+TDFFP VGW+IDR+SGV 
Sbjct: 181 TFTANITTRIAFGKSFRGSGLDNGNFHQVIRRAIAALGSLSMTDFFPNVGWVIDRLSGVS 240

Query: 241 GRLEKSFAELDAFLQHVVDDRINFMATSEN-ENIIDVLLRMERERSESDALQLTRDCIKA 300
           GRLEKSFAELDAFLQ VVDD INF ATS+N ENI+DVLL MER+ S+ +ALQLTRDCIKA
Sbjct: 241 GRLEKSFAELDAFLQQVVDDHINFKATSQNEENIVDVLLEMERDCSKPNALQLTRDCIKA 300

Query: 301 IIMDIFIAGVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRM 360
           +IMDIFIAGVNPGAGTIIWAM EL+RNPRVMKKLQ EIR+ +KE+QVKE+DLEKLQYL+M
Sbjct: 301 LIMDIFIAGVNPGAGTIIWAMTELVRNPRVMKKLQDEIRTSVKENQVKENDLEKLQYLKM 360

Query: 361 VVKEVLRLHAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPE 420
           V+KEVLRLHAPAPLLLPRET+SHFKLNGYDI    HL+VNVWAIGRD D W+NPEEFLPE
Sbjct: 361 VMKEVLRLHAPAPLLLPRETLSHFKLNGYDIDLKAHLYVNVWAIGRDLDYWTNPEEFLPE 420

Query: 421 RFMESCVDFRGQNFELLPFGAGRRSCPGVNMGAITVELILANLLLCFDWKLPDGMKEDVD 480
           RF+ S +D++GQNFE LPFGAGRR CPG+NMG +T+EL LANLLL FDWKLP+GMK DVD
Sbjct: 421 RFIGSTIDYKGQNFEYLPFGAGRRICPGMNMGIVTMELALANLLLLFDWKLPNGMK-DVD 480

Query: 481 MEEDAGLTVAKKSPLKLVPVCY 482
           MEEDAGLTV+KK PLKL+PV Y
Sbjct: 481 MEEDAGLTVSKKLPLKLIPVGY 501

BLAST of Moc09g41000 vs. NCBI nr
Match: XP_038892117.1 (cytochrome P450 71B26-like [Benincasa hispida] >XP_038892118.1 cytochrome P450 71B26-like [Benincasa hispida] >XP_038892119.1 cytochrome P450 71B26-like [Benincasa hispida])

HSP 1 Score: 736.1 bits (1899), Expect = 2.0e-208
Identity = 382/506 (75.49%), Postives = 424/506 (83.79%), Query Frame = 0

Query: 1   MFNLDQMMHSLSIWVSLLLLLASL-----------------------LLGHLHLIGSIPH 60
           MFNLDQMMH+LSI V  L LL  L                       LLGHLHLIGS+PH
Sbjct: 1   MFNLDQMMHNLSIGVVSLFLLLVLFFLLLLKTKTHNKKLPPGPPKLPLLGHLHLIGSLPH 60

Query: 61  RSLWKLSRKYGSVMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFL 120
           RSLWKLSRKYG +MLLKL S+PT+VISSA AAREVLQVHDLACCSRP LAASARFSYNFL
Sbjct: 61  RSLWKLSRKYGPIMLLKLVSVPTLVISSAEAAREVLQVHDLACCSRPLLAASARFSYNFL 120

Query: 121 DIGLSPYSDHWREVRKISVLELFSARRVQSFQLIREEEVGLLLKSLFESSSSAAPVDLSE 180
           DIGLSPYSDHW+EVRKI VLELF+ARRVQSF LIREEEV LLLKS+ +SSS A PVDLSE
Sbjct: 121 DIGLSPYSDHWKEVRKICVLELFNARRVQSFHLIREEEVALLLKSIAQSSSLAMPVDLSE 180

Query: 181 KSYTLTANIMTRIAFGKSFRGGELDNGNFHEVIRRAIAALASFSVTDFFPTVGWIIDRVS 240
           +SYTLTANI+TRIAFGK FRGG LDNGNF  +IRRAIAAL SFS+TDFFP VGWIIDR+S
Sbjct: 181 ESYTLTANIITRIAFGKRFRGGGLDNGNFQRLIRRAIAALGSFSMTDFFPCVGWIIDRLS 240

Query: 241 GVHGRLEKSFAELDAFLQHVVDDRINFMATSENENIIDVLLRMERERSE-SDALQLTRDC 300
           GV+GRLEKSFAELDAF Q VV+      A+   +NI+DVLL MER+ SE  DAL+LT DC
Sbjct: 241 GVNGRLEKSFAELDAFFQQVVEG-----ASQNEQNIVDVLLEMERDHSELDDALKLTTDC 300

Query: 301 IKAIIMDIFIAGVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCIKEDQVKESDLEKLQY 360
           IKAIIMDIFIAGVNPGA TIIW MAEL+++PRVMKKLQ EIRSCIKED VKES LEKLQY
Sbjct: 301 IKAIIMDIFIAGVNPGAVTIIWVMAELVKHPRVMKKLQDEIRSCIKEDLVKESHLEKLQY 360

Query: 361 LRMVVKEVLRLHAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWAIGRDPDSWSNPEEF 420
           L+MVVKEVLRLH PA LLLPRET+SHFKL GYDI P  HLHVNVWAIGRDP+ W+ PEEF
Sbjct: 361 LKMVVKEVLRLHPPASLLLPRETISHFKLKGYDIDPKTHLHVNVWAIGRDPNYWTRPEEF 420

Query: 421 LPERFMESCVDFRGQNFELLPFGAGRRSCPGVNMGAITVELILANLLLCFDWKLPDGMK- 480
           LPERF+ + +D++GQNFEL+PFGAGRR CPG+NMG +T+EL LANLLL F+WKLPDG+K 
Sbjct: 421 LPERFVGNNIDYKGQNFELVPFGAGRRICPGMNMGIVTMELALANLLLKFNWKLPDGIKE 480

Query: 481 EDVDMEEDAGLTVAKKSPLKLVPVCY 482
           ED+DMEEDAGLTVAKKSPLKL+P+ Y
Sbjct: 481 EDLDMEEDAGLTVAKKSPLKLIPISY 501

BLAST of Moc09g41000 vs. ExPASy Swiss-Prot
Match: Q9LIP6 (Cytochrome P450 71B34 OS=Arabidopsis thaliana OX=3702 GN=CYP71B34 PE=2 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 4.2e-137
Identity = 240/457 (52.52%), Postives = 334/457 (73.09%), Query Frame = 0

Query: 25  LLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRP 84
           ++G+LH +G +PH+SLWKLS+KYG VMLLKLG +PT+++SS+  A++ L++HDL CCSRP
Sbjct: 40  IIGNLHQLGELPHQSLWKLSKKYGPVMLLKLGRVPTVIVSSSETAKQALKIHDLHCCSRP 99

Query: 85  RLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQSFQLIREEEVGLLLKSLF 144
             A +   SYN+LDI  SPY D+W+EVRK++V ELFS+++V S Q I++EEV  L+ S+ 
Sbjct: 100 GFAGARELSYNYLDIAFSPYDDYWKEVRKLAVQELFSSKQVHSIQPIKDEEVKKLIDSIS 159

Query: 145 ESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNFHEVIRRAIAALASFSVTD 204
           ES++   P++L++    LT +++ R AF  +F G  L++  F+ ++R A+  L SFS +D
Sbjct: 160 ESAAQKTPINLNKTLLALTVSVVCRTAFSVNFEGTVLNSERFNNIVREALEMLGSFSASD 219

Query: 205 FFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMATSE-NENIIDVLLRMERE 264
           F P VG IID ++G+ GR E+S  +LDAF + + D  ++     E +E+ +D+LLR+E+E
Sbjct: 220 FIPYVGRIIDLLTGLQGRRERSMRDLDAFYEQMFD--LHKQKKEEGSEDFVDLLLRLEKE 279

Query: 265 RSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCIK- 324
            +     +LTR+ IKAI+MD+ +AG++  A T+ WAMAEL +NPRVMKK+Q EIRS IK 
Sbjct: 280 EAVLGNDKLTRNHIKAILMDVLLAGMDTSAITMTWAMAELAKNPRVMKKVQSEIRSQIKN 339

Query: 325 EDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWA 384
           ++++   D +KL+YL+MV+KE  RLH   PLL+PRE MS F++NGY I     LHVNVWA
Sbjct: 340 KERISFDDTDKLEYLKMVIKETWRLHPTTPLLIPREAMSEFEINGYTIPVKTRLHVNVWA 399

Query: 385 IGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCPGVNMGAITVELILANL 444
           IGRDPD+W +PE FLPERF ++ +D +GQ+FELLPFG GRR CP V MG   VE  LANL
Sbjct: 400 IGRDPDTWKDPEVFLPERFTDNNIDAKGQHFELLPFGGGRRMCPAVYMGTTMVEFGLANL 459

Query: 445 LLCFDWKLPDGMK-EDVDMEEDAGLTVAKKSPLKLVP 479
           L  FDWKLP+GMK +D+DMEE  GLTV KK+ L LVP
Sbjct: 460 LYHFDWKLPEGMKVDDIDMEEAPGLTVNKKNELILVP 494

BLAST of Moc09g41000 vs. ExPASy Swiss-Prot
Match: Q9LIP5 (Cytochrome P450 71B35 OS=Arabidopsis thaliana OX=3702 GN=CYP71B35 PE=2 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.4e-135
Identity = 239/457 (52.30%), Postives = 329/457 (71.99%), Query Frame = 0

Query: 25  LLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRP 84
           ++G+LH IG +PH++LWKLS+KYG VM L LG +PT+V+SS+  AR+VL+VHDL CC+RP
Sbjct: 39  IIGNLHQIGELPHQTLWKLSKKYGPVMHLMLGRVPTVVVSSSDTARQVLRVHDLHCCTRP 98

Query: 85  RLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQSFQLIREEEVGLLLKSLF 144
            L+     SYN+LDI  SPY D+W+EVRK+ V ELFS ++V S Q I++EEV  ++ S+ 
Sbjct: 99  SLSGPRELSYNYLDIAFSPYDDYWKEVRKLCVQELFSTKQVHSIQPIKDEEVKKMIDSIA 158

Query: 145 ESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNFHEVIRRAIAALASFSVTD 204
           ES+S   PV+L+ K   LT +++ R AFG SF G  L++  F++++R A+  L SFS  D
Sbjct: 159 ESASQKNPVNLNNKCLELTVSVVCRTAFGVSFEGTVLNSDRFNKIVREALEMLGSFSAAD 218

Query: 205 FFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMATSE-NENIIDVLLRMERE 264
           F P VGWIID ++G+ GR E+S  +L+AF + + D  ++     E NE+ +D+LLR+E+E
Sbjct: 219 FIPYVGWIIDVLTGLQGRRERSKRDLNAFFEQMFD--LHKEGKKEGNEDFVDLLLRLEKE 278

Query: 265 RSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCI-K 324
            +     +LTR+ IKAI++D+ +AG++  A T+ WAM EL RNPRVMKK+Q EIR+ +  
Sbjct: 279 EAVLGNDKLTRNHIKAILLDVLLAGIDTSAITMTWAMTELARNPRVMKKVQSEIRTQMGN 338

Query: 325 EDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWA 384
              +   D+++L+YL+MV+KE  RLH   PLLLPRE MS F +NGY I     LHVNVWA
Sbjct: 339 RSMISFEDMDQLEYLKMVIKETWRLHPTTPLLLPREAMSEFDINGYTIPVKTRLHVNVWA 398

Query: 385 IGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCPGVNMGAITVELILANL 444
           IGRDPD+W +PE FLPERFM++ +D +GQ+FELLPFG GRR CP + MG   VE  LANL
Sbjct: 399 IGRDPDTWKDPEVFLPERFMDNNIDAKGQHFELLPFGGGRRICPAIYMGTTMVEFGLANL 458

Query: 445 LLCFDWKLPDGMK-EDVDMEEDAGLTVAKKSPLKLVP 479
           L  FDWKLP+G++ +D+D+EE  GLTV KK+ L LVP
Sbjct: 459 LYHFDWKLPEGVEVKDIDVEEAPGLTVNKKNELLLVP 493

BLAST of Moc09g41000 vs. ExPASy Swiss-Prot
Match: Q9LIP4 (Cytochrome P450 71B36 OS=Arabidopsis thaliana OX=3702 GN=CYP71B36 PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.4e-135
Identity = 238/460 (51.74%), Postives = 329/460 (71.52%), Query Frame = 0

Query: 25  LLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRP 84
           ++G+LH +G +PH+SLW+LS+KYG VMLLK GSIPT+V+SS+  A++VL++HDL CCSRP
Sbjct: 40  IIGNLHQLGELPHQSLWRLSKKYGHVMLLKFGSIPTVVVSSSETAKQVLKIHDLHCCSRP 99

Query: 85  RLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQSFQLIREEEVGLLLKSLF 144
            LA     SYN+LDI  SP+ D+W+E+R+I V ELFS +RVQSFQ I+E+EV  L+ S+ 
Sbjct: 100 SLAGPRALSYNYLDIAFSPFDDYWKELRRICVQELFSVKRVQSFQPIKEDEVKKLIDSVS 159

Query: 145 ESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNFHEVIRRAIAALASFSVTD 204
           ES+S   PV+LSEK  +LT  +  +  FG +F+G  L++  F ++I      L SFS +D
Sbjct: 160 ESASQGTPVNLSEKFTSLTVRVTCKATFGVNFQGTVLNSDRFEKLIHDTYLFLGSFSASD 219

Query: 205 FFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMATSEN-ENIIDVLLRMERE 264
           +FP  GWIID ++G+HG+ E+S   LDAF + + D  ++     E  E+ +D+LLR+E+E
Sbjct: 220 YFPNGGWIIDWLTGLHGQRERSVRALDAFYEQMFD--LHKQGNKEGVEDFVDLLLRLEKE 279

Query: 265 RSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCI-K 324
            +     +LTR+ IKAI+M++ I G+   A T+ WAM EL+RNPRVMKK+Q EIR+ I K
Sbjct: 280 ETVIGYGKLTRNHIKAILMNVLIGGIGTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGK 339

Query: 325 EDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWA 384
           +  +   D+++L YL+MV+ E  RLH P+P L+PR+ MS F+LN Y I     L+VNVWA
Sbjct: 340 KSMITLDDIDQLHYLKMVINETWRLHPPSPFLIPRQVMSEFELNDYVIPVKTRLYVNVWA 399

Query: 385 IGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCPGVNMGAITVELILANL 444
           IGRDPD+W +PEEFLPERF+ S +D +GQ+FELLPFG+GRR CP + MG   VE  LAN+
Sbjct: 400 IGRDPDTWKDPEEFLPERFVNSSIDAKGQHFELLPFGSGRRMCPAMYMGTTMVEFGLANM 459

Query: 445 LLCFDWKLPDGM-KEDVDMEEDAGLTVAKKSPLKLVPVCY 482
           L  FDWK+P GM  ED+D+EE  GL  +KK+ L LVP+ Y
Sbjct: 460 LYHFDWKIPVGMVAEDIDLEESPGLNASKKNELVLVPLKY 497

BLAST of Moc09g41000 vs. ExPASy Swiss-Prot
Match: Q9LTL0 (Cytochrome P450 71B26 OS=Arabidopsis thaliana OX=3702 GN=CYP71B26 PE=2 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 1.8e-135
Identity = 240/459 (52.29%), Postives = 331/459 (72.11%), Query Frame = 0

Query: 25  LLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRP 84
           ++G+LH +G + H+SLWKLS+KYG VMLLKLG +PT+++SS+  A++ L+ +DL CCSRP
Sbjct: 40  IIGNLHQLGELQHQSLWKLSKKYGPVMLLKLGKVPTLILSSSETAKQALRDYDLHCCSRP 99

Query: 85  RLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQSFQLIREEEVGLLLKSLF 144
            LA     SYN LD+  SPY+++W+E+RK+   ELFSA ++QS Q I++EEV  ++ S+ 
Sbjct: 100 SLAGGRELSYNNLDMSSSPYNEYWKELRKLCSQELFSANKIQSIQPIKDEEVKKVIDSIA 159

Query: 145 ESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNFHEVIRRAIAALASFSVTD 204
           ESSS   PV+LS+    LT +++ + AFG SF G  L++  F++++R     L SFS +D
Sbjct: 160 ESSSLKNPVNLSKTFLALTTSVVCKAAFGVSFEGSVLNSDRFNKLVRDTFEMLGSFSASD 219

Query: 205 FFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMATSENENIIDVLLRMERER 264
           F P VGWIID+ +G+ G  +KSF +LDAF + + D          +E+++DVLLR+E+E 
Sbjct: 220 FIPYVGWIIDKFNGLQGWRKKSFRDLDAFYEQIFDLHKEEKEVG-SEDLVDVLLRLEKEE 279

Query: 265 SESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCIK-E 324
                 +LTR+ IKAI+M+I + G++  A T+ WAMAEL +NPRVMKK+Q EIR+ IK +
Sbjct: 280 IVVGNGKLTRNHIKAILMNILLGGIDTSAITMTWAMAELAKNPRVMKKVQAEIRNQIKNK 339

Query: 325 DQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWAI 384
           +++   D +KL+YL+MV+KE  RLH P PLLLPR+ ++ F++NGY I     LHVNVWAI
Sbjct: 340 ERISFDDTDKLEYLKMVIKETWRLHPPTPLLLPRDVITEFEINGYTIPAKTRLHVNVWAI 399

Query: 385 GRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCPGVNMGAITVELILANLL 444
           GRDPD+W +PE FLPERF +S +D +GQNFELL FG+GRR CPG+ MG   VE  LAN+L
Sbjct: 400 GRDPDTWKDPEMFLPERFNDSNIDAKGQNFELLSFGSGRRICPGLYMGTTMVEFGLANML 459

Query: 445 LCFDWKLPDGM-KEDVDMEEDAGLTVAKKSPLKLVPVCY 482
             FDWKLP+GM  ED+DMEE  GLTV+KKS L LVPV Y
Sbjct: 460 YHFDWKLPEGMVVEDIDMEEAPGLTVSKKSELVLVPVKY 497

BLAST of Moc09g41000 vs. ExPASy Swiss-Prot
Match: O64718 (Cytochrome P450 71B9 OS=Arabidopsis thaliana OX=3702 GN=CYP71B9 PE=2 SV=3)

HSP 1 Score: 483.8 bits (1244), Expect = 2.3e-135
Identity = 243/463 (52.48%), Postives = 327/463 (70.63%), Query Frame = 0

Query: 25  LLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRP 84
           ++G+LH +G +PH+SLW LS+ YG VMLLKLGS+PT+V+SS+  A++VL+++DL CCSRP
Sbjct: 40  IIGNLHQLGELPHQSLWSLSKTYGPVMLLKLGSVPTVVVSSSETAKQVLKINDLHCCSRP 99

Query: 85  RLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQSFQLIREEEVGLLLKSLF 144
            LA +   SYN+LDI  SP+ D+W+E+R+I V ELFSA+RV S Q I+EEEV  L+ S  
Sbjct: 100 SLAGAKELSYNYLDIAFSPFDDYWKELRRICVQELFSAKRVHSIQPIKEEEVRKLIVSAT 159

Query: 145 ESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNFHEVIRRAIAALASFSVTD 204
           ES+S  +PV+LSEK   LT +++ + AF   F    L+N  F ++I  A   L SFS ++
Sbjct: 160 ESASQKSPVNLSEKFLDLTVSVICKAAFSLDFHTSVLNNDGFDKLIHDAFLFLGSFSASN 219

Query: 205 FFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMATSEN----ENIIDVLLRM 264
           FFP  GWIID ++G+  R EKS  +LD F Q + D     +   EN    E+ +D+LL++
Sbjct: 220 FFPNGGWIIDWLTGLQRRREKSVKDLDVFYQQMFD-----LHKQENKQGVEDFVDLLLKL 279

Query: 265 ERERSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRNPRVMKKLQYEIRS- 324
           E+E +     +LTR+ +KAI+M++ +  +N  A T+ WAMAELIRNPRVMKK+Q EIR+ 
Sbjct: 280 EKEETVLGYGKLTRNHVKAILMNVLLGAINTSAMTMTWAMAELIRNPRVMKKVQSEIRNQ 339

Query: 325 CIKEDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLNGYDIAPMVHLHVN 384
            I +  +   D++ L YL+MV+KE  RLH P PLLLPRE MS F++NGY I P   L+VN
Sbjct: 340 MINKSVITLDDIDHLPYLKMVIKETWRLHPPVPLLLPREVMSEFEINGYKIQPKTLLYVN 399

Query: 385 VWAIGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCPGVNMGAITVELIL 444
           VWAIGRDPDSW + + F PERFM++ +D +GQNFELLPFG+GRR CPG+ MG   VE  L
Sbjct: 400 VWAIGRDPDSWKDADMFYPERFMDNNIDAKGQNFELLPFGSGRRICPGMYMGTTMVEFGL 459

Query: 445 ANLLLCFDWKLPDGM-KEDVDMEEDAGLTVAKKSPLKLVPVCY 482
           AN+L  FDW++PDGM  ED+DMEE  GL V KK+ L LVPV Y
Sbjct: 460 ANMLYQFDWEVPDGMVVEDIDMEESPGLAVGKKNELLLVPVKY 497

BLAST of Moc09g41000 vs. ExPASy TrEMBL
Match: A0A6J1DEX7 (cytochrome P450 71B34-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019806 PE=3 SV=1)

HSP 1 Score: 981.9 bits (2537), Expect = 1.0e-282
Identity = 494/494 (100.00%), Postives = 494/494 (100.00%), Query Frame = 0

Query: 1   MFNLDQMMHSLSIWVSLLLLLASLLLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPT 60
           MFNLDQMMHSLSIWVSLLLLLASLLLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPT
Sbjct: 1   MFNLDQMMHSLSIWVSLLLLLASLLLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPT 60

Query: 61  IVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIGLSPYSDHWREVRKISVLELF 120
           IVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIGLSPYSDHWREVRKISVLELF
Sbjct: 61  IVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIGLSPYSDHWREVRKISVLELF 120

Query: 121 SARRVQSFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGE 180
           SARRVQSFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGE
Sbjct: 121 SARRVQSFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGE 180

Query: 181 LDNGNFHEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDD 240
           LDNGNFHEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDD
Sbjct: 181 LDNGNFHEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDD 240

Query: 241 RINFMATSENENIIDVLLRMERERSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAM 300
           RINFMATSENENIIDVLLRMERERSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAM
Sbjct: 241 RINFMATSENENIIDVLLRMERERSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAM 300

Query: 301 AELIRNPRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETM 360
           AELIRNPRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETM
Sbjct: 301 AELIRNPRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETM 360

Query: 361 SHFKLNGYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGA 420
           SHFKLNGYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGA
Sbjct: 361 SHFKLNGYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGA 420

Query: 421 GRRSCPGVNMGAITVELILANLLLCFDWKLPDGMKEDVDMEEDAGLTVAKKSPLKLVPVC 480
           GRRSCPGVNMGAITVELILANLLLCFDWKLPDGMKEDVDMEEDAGLTVAKKSPLKLVPVC
Sbjct: 421 GRRSCPGVNMGAITVELILANLLLCFDWKLPDGMKEDVDMEEDAGLTVAKKSPLKLVPVC 480

Query: 481 YGFGSTHDHECQVP 495
           YGFGSTHDHECQVP
Sbjct: 481 YGFGSTHDHECQVP 494

BLAST of Moc09g41000 vs. ExPASy TrEMBL
Match: A0A6J1DCP0 (cytochrome P450 71B34-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019806 PE=3 SV=1)

HSP 1 Score: 969.9 bits (2506), Expect = 3.9e-279
Identity = 488/488 (100.00%), Postives = 488/488 (100.00%), Query Frame = 0

Query: 7   MMHSLSIWVSLLLLLASLLLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSA 66
           MMHSLSIWVSLLLLLASLLLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSA
Sbjct: 1   MMHSLSIWVSLLLLLASLLLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSA 60

Query: 67  AAAREVLQVHDLACCSRPRLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQ 126
           AAAREVLQVHDLACCSRPRLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQ
Sbjct: 61  AAAREVLQVHDLACCSRPRLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQ 120

Query: 127 SFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNF 186
           SFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNF
Sbjct: 121 SFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNF 180

Query: 187 HEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMA 246
           HEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMA
Sbjct: 181 HEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMA 240

Query: 247 TSENENIIDVLLRMERERSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRN 306
           TSENENIIDVLLRMERERSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRN
Sbjct: 241 TSENENIIDVLLRMERERSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRN 300

Query: 307 PRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLN 366
           PRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLN
Sbjct: 301 PRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLN 360

Query: 367 GYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCP 426
           GYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCP
Sbjct: 361 GYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCP 420

Query: 427 GVNMGAITVELILANLLLCFDWKLPDGMKEDVDMEEDAGLTVAKKSPLKLVPVCYGFGST 486
           GVNMGAITVELILANLLLCFDWKLPDGMKEDVDMEEDAGLTVAKKSPLKLVPVCYGFGST
Sbjct: 421 GVNMGAITVELILANLLLCFDWKLPDGMKEDVDMEEDAGLTVAKKSPLKLVPVCYGFGST 480

Query: 487 HDHECQVP 495
           HDHECQVP
Sbjct: 481 HDHECQVP 488

BLAST of Moc09g41000 vs. ExPASy TrEMBL
Match: A0A6J1G094 (cytochrome P450 71B37-like OS=Cucurbita moschata OX=3662 GN=LOC111449540 PE=3 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 7.8e-219
Identity = 398/502 (79.28%), Postives = 435/502 (86.65%), Query Frame = 0

Query: 1   MFNLDQMMHSLSIW-VSLLLLLASL-------------------LLGHLHLIGSIPHRSL 60
           M  LD+MMHSLSIW VSLLLLL  L                   LLGHLHLIGS+PHRSL
Sbjct: 1   MLGLDRMMHSLSIWVVSLLLLLFFLLLKTKTHNKKLPPGPPKLPLLGHLHLIGSLPHRSL 60

Query: 61  WKLSRKYGSVMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIG 120
           WKLSR YG +MLLKLGSIPTIVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIG
Sbjct: 61  WKLSRTYGPIMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRPRLAASARFSYNFLDIG 120

Query: 121 LSPYSDHWREVRKISVLELFSARRVQSFQLIREEEVGLLLKSLFESSSSAAPVDLSEKSY 180
           LSPYSDHWREVRKI VLELFSARRVQSF+ IREEEV LLLKS+ +SSS   PVDLSE+SY
Sbjct: 121 LSPYSDHWREVRKICVLELFSARRVQSFKSIREEEVALLLKSISQSSSLEIPVDLSEESY 180

Query: 181 TLTANIMTRIAFGKSFRGGELDNGNFHEVIRRAIAALASFSVTDFFPTVGWIIDRVSGVH 240
           T TANI TRIAFGKSFRG  LDNGNFH+VIRRAIAAL S S+TDFFP VGW+IDR+SGV 
Sbjct: 181 TFTANITTRIAFGKSFRGSGLDNGNFHQVIRRAIAALGSLSMTDFFPNVGWVIDRLSGVS 240

Query: 241 GRLEKSFAELDAFLQHVVDDRINFMATSEN-ENIIDVLLRMERERSESDALQLTRDCIKA 300
           GRLEKSFAELDAFLQ VVDD INF ATS+N ENI+DVLL MER+ S+ +ALQLTRDCIKA
Sbjct: 241 GRLEKSFAELDAFLQQVVDDHINFKATSQNEENIVDVLLEMERDCSKPNALQLTRDCIKA 300

Query: 301 IIMDIFIAGVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCIKEDQVKESDLEKLQYLRM 360
           +IMDIFIAGVNPGAGTIIWAM EL+RNPRVMKKLQ EIR+ +KE+QVKE+DLEKLQYL+M
Sbjct: 301 LIMDIFIAGVNPGAGTIIWAMTELVRNPRVMKKLQDEIRTSVKENQVKENDLEKLQYLKM 360

Query: 361 VVKEVLRLHAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWAIGRDPDSWSNPEEFLPE 420
           V+KEVLRLHAPAPLLLPRET+SHFKLNGYDI    HL+VNVWAIGRD D W+NPEEFLPE
Sbjct: 361 VMKEVLRLHAPAPLLLPRETLSHFKLNGYDIDLKAHLYVNVWAIGRDLDYWTNPEEFLPE 420

Query: 421 RFMESCVDFRGQNFELLPFGAGRRSCPGVNMGAITVELILANLLLCFDWKLPDGMKEDVD 480
           RF+ S +D++GQNFE LPFGAGRR CPG+NMG +T+EL LANLLL FDWKLP+GMK DVD
Sbjct: 421 RFIGSTIDYKGQNFEYLPFGAGRRICPGMNMGIVTMELALANLLLLFDWKLPNGMK-DVD 480

Query: 481 MEEDAGLTVAKKSPLKLVPVCY 482
           MEEDAGLTV+KK PLKL+PV Y
Sbjct: 481 MEEDAGLTVSKKLPLKLIPVGY 501

BLAST of Moc09g41000 vs. ExPASy TrEMBL
Match: A0A6J1GYQ4 (cytochrome P450 71B34-like OS=Cucurbita moschata OX=3662 GN=LOC111458729 PE=3 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 1.3e-197
Identity = 336/456 (73.68%), Postives = 398/456 (87.28%), Query Frame = 0

Query: 25  LLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRP 84
           LLGHLHL+GS+PHRSL +LS+KYG VMLL+LGS+PTIVISSAAAARE+ + HDLA CSRP
Sbjct: 46  LLGHLHLLGSLPHRSLSQLSKKYGPVMLLQLGSVPTIVISSAAAARELFKFHDLASCSRP 105

Query: 85  RLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQSFQLIREEEVGLLLKSLF 144
           RLA S R SYN+LDI  SPY DHWR VRKI +LELF++RRV+SF+ IREEEVG LL S+ 
Sbjct: 106 RLAGSGRLSYNYLDISFSPYGDHWRNVRKICMLELFTSRRVESFREIREEEVGRLLNSIS 165

Query: 145 ESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNFHEVIRRAIAALASFSVTD 204
           +SSSS+AP+DLSEKSY+LTANI+TR+AFG  F GG+LD+ +F  V+  A+AA+ SFS+TD
Sbjct: 166 QSSSSSAPIDLSEKSYSLTANIITRVAFGSIFSGGKLDDEHFQHVMHSALAAIGSFSMTD 225

Query: 205 FFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMATS-ENENIIDVLLRMERE 264
           F PT GWIIDR++GVHGRLEKSFAE+DAF QHVV+DRINF  +S + ENI+DVLLRMERE
Sbjct: 226 FIPTFGWIIDRLNGVHGRLEKSFAEMDAFFQHVVEDRINFKKSSKKEENIVDVLLRMERE 285

Query: 265 RSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCIKE 324
            SESDAL++T+DC+KA+IMDIF+AGV  GAGTI+WAM EL+RNPRVMKKLQ+EIRSCIKE
Sbjct: 286 SSESDALKVTKDCVKALIMDIFLAGVETGAGTIVWAMTELVRNPRVMKKLQHEIRSCIKE 345

Query: 325 DQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWAI 384
             VKE +LEKL+YL+MVVKEVLRLH P PLLLPRET+  FKLNGYDI P  HLH+NVWAI
Sbjct: 346 GSVKEINLEKLEYLKMVVKEVLRLHPPVPLLLPRETICPFKLNGYDIDPKSHLHINVWAI 405

Query: 385 GRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCPGVNMGAITVELILANLL 444
           GRDP SW++PEEF PERF+ES VD++GQNFEL+PFG GRR CPG+NMG +TVEL LANL 
Sbjct: 406 GRDPQSWTDPEEFFPERFIESNVDYKGQNFELIPFGGGRRICPGINMGTLTVELALANLF 465

Query: 445 LCFDWKLPDGMK-EDVDMEEDAGLTVAKKSPLKLVP 479
           LCFDWKLP+GMK ED+DMEE+ GLT+ KKSPLKL+P
Sbjct: 466 LCFDWKLPNGMKEEDIDMEEEVGLTITKKSPLKLIP 501

BLAST of Moc09g41000 vs. ExPASy TrEMBL
Match: A0A6J1DDC0 (cytochrome P450 71B34-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019732 PE=3 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 2.2e-197
Identity = 359/509 (70.53%), Postives = 414/509 (81.34%), Query Frame = 0

Query: 1   MFNLDQMMH----SLSIWVSLLLLLASL--------------------LLGHLHLIGSIP 60
           M +LDQ+MH    +L+I + LLLLL +                     LLGHLHLIGS+P
Sbjct: 1   MHSLDQLMHTILLTLAIPLFLLLLLKTKTKFSTQKNKNQLPPSPPKLPLLGHLHLIGSLP 60

Query: 61  HRSLWKLSRKYGSVMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRPRLAASARFSYNF 120
           HRSLWKLS KYG VM L LGS+PTI ISSAAAAR++ ++HDLA CSRP L+ S R SYNF
Sbjct: 61  HRSLWKLSTKYGPVMRLHLGSVPTIXISSAAAARDMFKLHDLASCSRPPLSGSGRLSYNF 120

Query: 121 LDIGLSPYSDHWREVRKISVLELFSARRVQSFQLIREEEVGLLLKSLFESSSSAAPVDLS 180
           LDI  SPY DHWREVRKI VLELFS RRVQSF+ +RE EVGLL+ S+ +SS+S A VD+S
Sbjct: 121 LDISFSPYGDHWREVRKICVLELFSVRRVQSFRGVREHEVGLLINSIAQSSTSGALVDVS 180

Query: 181 EKSYTLTANIMTRIAFGKSFRGGELDNGNFHEVIRRAIAALASFSVTDFFPTVGWIIDRV 240
           EKSY+LTA+I+TR+AFGKSFRGGELDN  F EVIRRA+  + SFS TDFFP+ GWIID +
Sbjct: 181 EKSYSLTASIITRVAFGKSFRGGELDNEKFEEVIRRALVVMGSFSATDFFPSFGWIIDWM 240

Query: 241 SGVHGRLEKSFAELDAFLQHVVDDRINFMATSENE-NIIDVLLRMERERSESDALQLTRD 300
           SGV GRLEKSF ELD+F QHV+D R+ F AT +++ NI+DVLL+MERERSE DALQLTRD
Sbjct: 241 SGVRGRLEKSFLELDSFFQHVIDHRLKFGATCDDDNNILDVLLKMERERSEFDALQLTRD 300

Query: 301 CIKAIIMDIFIAGVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCIKEDQ--VKESDLEK 360
           CIKA+ MDIFIAGV+ GAGTI+WAM EL RNPRVMKKLQ EIRS IKE+   VKES LEK
Sbjct: 301 CIKALTMDIFIAGVDTGAGTIVWAMTELARNPRVMKKLQDEIRSSIKEEDRVVKESHLEK 360

Query: 361 LQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWAIGRDPDSWSNP 420
           L+YL+MVVKEVLRLH PAPLLLPRETMSHFKL GYDI P  HLHVN+WAIGRDP+SW++P
Sbjct: 361 LEYLKMVVKEVLRLHPPAPLLLPRETMSHFKLGGYDIEPKAHLHVNIWAIGRDPESWTDP 420

Query: 421 EEFLPERFMESCVDFRGQNFELLPFGAGRRSCPGVNMGAITVELILANLLLCFDWKLPDG 480
           +EFLPERF+ S +DFRGQNFELLPFGAGRRSCPG++M  + VEL LAN+LLCFDWKLP G
Sbjct: 421 DEFLPERFVGSNIDFRGQNFELLPFGAGRRSCPGMHMATVAVELALANMLLCFDWKLPQG 480

Query: 481 MK-EDVDMEEDAGLTVAKKSPLKLVPVCY 482
           MK ED+DMEEDAGLTV KKSPL L+PV Y
Sbjct: 481 MKEEDLDMEEDAGLTVTKKSPLHLIPVEY 509

BLAST of Moc09g41000 vs. TAIR 10
Match: AT3G26300.1 (cytochrome P450, family 71, subfamily B, polypeptide 34 )

HSP 1 Score: 489.6 bits (1259), Expect = 3.0e-138
Identity = 240/457 (52.52%), Postives = 334/457 (73.09%), Query Frame = 0

Query: 25  LLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRP 84
           ++G+LH +G +PH+SLWKLS+KYG VMLLKLG +PT+++SS+  A++ L++HDL CCSRP
Sbjct: 40  IIGNLHQLGELPHQSLWKLSKKYGPVMLLKLGRVPTVIVSSSETAKQALKIHDLHCCSRP 99

Query: 85  RLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQSFQLIREEEVGLLLKSLF 144
             A +   SYN+LDI  SPY D+W+EVRK++V ELFS+++V S Q I++EEV  L+ S+ 
Sbjct: 100 GFAGARELSYNYLDIAFSPYDDYWKEVRKLAVQELFSSKQVHSIQPIKDEEVKKLIDSIS 159

Query: 145 ESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNFHEVIRRAIAALASFSVTD 204
           ES++   P++L++    LT +++ R AF  +F G  L++  F+ ++R A+  L SFS +D
Sbjct: 160 ESAAQKTPINLNKTLLALTVSVVCRTAFSVNFEGTVLNSERFNNIVREALEMLGSFSASD 219

Query: 205 FFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMATSE-NENIIDVLLRMERE 264
           F P VG IID ++G+ GR E+S  +LDAF + + D  ++     E +E+ +D+LLR+E+E
Sbjct: 220 FIPYVGRIIDLLTGLQGRRERSMRDLDAFYEQMFD--LHKQKKEEGSEDFVDLLLRLEKE 279

Query: 265 RSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCIK- 324
            +     +LTR+ IKAI+MD+ +AG++  A T+ WAMAEL +NPRVMKK+Q EIRS IK 
Sbjct: 280 EAVLGNDKLTRNHIKAILMDVLLAGMDTSAITMTWAMAELAKNPRVMKKVQSEIRSQIKN 339

Query: 325 EDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWA 384
           ++++   D +KL+YL+MV+KE  RLH   PLL+PRE MS F++NGY I     LHVNVWA
Sbjct: 340 KERISFDDTDKLEYLKMVIKETWRLHPTTPLLIPREAMSEFEINGYTIPVKTRLHVNVWA 399

Query: 385 IGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCPGVNMGAITVELILANL 444
           IGRDPD+W +PE FLPERF ++ +D +GQ+FELLPFG GRR CP V MG   VE  LANL
Sbjct: 400 IGRDPDTWKDPEVFLPERFTDNNIDAKGQHFELLPFGGGRRMCPAVYMGTTMVEFGLANL 459

Query: 445 LLCFDWKLPDGMK-EDVDMEEDAGLTVAKKSPLKLVP 479
           L  FDWKLP+GMK +D+DMEE  GLTV KK+ L LVP
Sbjct: 460 LYHFDWKLPEGMKVDDIDMEEAPGLTVNKKNELILVP 494

BLAST of Moc09g41000 vs. TAIR 10
Match: AT3G26310.1 (cytochrome P450, family 71, subfamily B, polypeptide 35 )

HSP 1 Score: 484.6 bits (1246), Expect = 9.7e-137
Identity = 239/457 (52.30%), Postives = 329/457 (71.99%), Query Frame = 0

Query: 25  LLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRP 84
           ++G+LH IG +PH++LWKLS+KYG VM L LG +PT+V+SS+  AR+VL+VHDL CC+RP
Sbjct: 39  IIGNLHQIGELPHQTLWKLSKKYGPVMHLMLGRVPTVVVSSSDTARQVLRVHDLHCCTRP 98

Query: 85  RLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQSFQLIREEEVGLLLKSLF 144
            L+     SYN+LDI  SPY D+W+EVRK+ V ELFS ++V S Q I++EEV  ++ S+ 
Sbjct: 99  SLSGPRELSYNYLDIAFSPYDDYWKEVRKLCVQELFSTKQVHSIQPIKDEEVKKMIDSIA 158

Query: 145 ESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNFHEVIRRAIAALASFSVTD 204
           ES+S   PV+L+ K   LT +++ R AFG SF G  L++  F++++R A+  L SFS  D
Sbjct: 159 ESASQKNPVNLNNKCLELTVSVVCRTAFGVSFEGTVLNSDRFNKIVREALEMLGSFSAAD 218

Query: 205 FFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMATSE-NENIIDVLLRMERE 264
           F P VGWIID ++G+ GR E+S  +L+AF + + D  ++     E NE+ +D+LLR+E+E
Sbjct: 219 FIPYVGWIIDVLTGLQGRRERSKRDLNAFFEQMFD--LHKEGKKEGNEDFVDLLLRLEKE 278

Query: 265 RSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCI-K 324
            +     +LTR+ IKAI++D+ +AG++  A T+ WAM EL RNPRVMKK+Q EIR+ +  
Sbjct: 279 EAVLGNDKLTRNHIKAILLDVLLAGIDTSAITMTWAMTELARNPRVMKKVQSEIRTQMGN 338

Query: 325 EDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWA 384
              +   D+++L+YL+MV+KE  RLH   PLLLPRE MS F +NGY I     LHVNVWA
Sbjct: 339 RSMISFEDMDQLEYLKMVIKETWRLHPTTPLLLPREAMSEFDINGYTIPVKTRLHVNVWA 398

Query: 385 IGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCPGVNMGAITVELILANL 444
           IGRDPD+W +PE FLPERFM++ +D +GQ+FELLPFG GRR CP + MG   VE  LANL
Sbjct: 399 IGRDPDTWKDPEVFLPERFMDNNIDAKGQHFELLPFGGGRRICPAIYMGTTMVEFGLANL 458

Query: 445 LLCFDWKLPDGMK-EDVDMEEDAGLTVAKKSPLKLVP 479
           L  FDWKLP+G++ +D+D+EE  GLTV KK+ L LVP
Sbjct: 459 LYHFDWKLPEGVEVKDIDVEEAPGLTVNKKNELLLVP 493

BLAST of Moc09g41000 vs. TAIR 10
Match: AT3G26320.1 (cytochrome P450, family 71, subfamily B, polypeptide 36 )

HSP 1 Score: 484.6 bits (1246), Expect = 9.7e-137
Identity = 238/460 (51.74%), Postives = 329/460 (71.52%), Query Frame = 0

Query: 25  LLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRP 84
           ++G+LH +G +PH+SLW+LS+KYG VMLLK GSIPT+V+SS+  A++VL++HDL CCSRP
Sbjct: 40  IIGNLHQLGELPHQSLWRLSKKYGHVMLLKFGSIPTVVVSSSETAKQVLKIHDLHCCSRP 99

Query: 85  RLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQSFQLIREEEVGLLLKSLF 144
            LA     SYN+LDI  SP+ D+W+E+R+I V ELFS +RVQSFQ I+E+EV  L+ S+ 
Sbjct: 100 SLAGPRALSYNYLDIAFSPFDDYWKELRRICVQELFSVKRVQSFQPIKEDEVKKLIDSVS 159

Query: 145 ESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNFHEVIRRAIAALASFSVTD 204
           ES+S   PV+LSEK  +LT  +  +  FG +F+G  L++  F ++I      L SFS +D
Sbjct: 160 ESASQGTPVNLSEKFTSLTVRVTCKATFGVNFQGTVLNSDRFEKLIHDTYLFLGSFSASD 219

Query: 205 FFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMATSEN-ENIIDVLLRMERE 264
           +FP  GWIID ++G+HG+ E+S   LDAF + + D  ++     E  E+ +D+LLR+E+E
Sbjct: 220 YFPNGGWIIDWLTGLHGQRERSVRALDAFYEQMFD--LHKQGNKEGVEDFVDLLLRLEKE 279

Query: 265 RSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCI-K 324
            +     +LTR+ IKAI+M++ I G+   A T+ WAM EL+RNPRVMKK+Q EIR+ I K
Sbjct: 280 ETVIGYGKLTRNHIKAILMNVLIGGIGTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGK 339

Query: 325 EDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWA 384
           +  +   D+++L YL+MV+ E  RLH P+P L+PR+ MS F+LN Y I     L+VNVWA
Sbjct: 340 KSMITLDDIDQLHYLKMVINETWRLHPPSPFLIPRQVMSEFELNDYVIPVKTRLYVNVWA 399

Query: 385 IGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCPGVNMGAITVELILANL 444
           IGRDPD+W +PEEFLPERF+ S +D +GQ+FELLPFG+GRR CP + MG   VE  LAN+
Sbjct: 400 IGRDPDTWKDPEEFLPERFVNSSIDAKGQHFELLPFGSGRRMCPAMYMGTTMVEFGLANM 459

Query: 445 LLCFDWKLPDGM-KEDVDMEEDAGLTVAKKSPLKLVPVCY 482
           L  FDWK+P GM  ED+D+EE  GL  +KK+ L LVP+ Y
Sbjct: 460 LYHFDWKIPVGMVAEDIDLEESPGLNASKKNELVLVPLKY 497

BLAST of Moc09g41000 vs. TAIR 10
Match: AT3G26290.1 (cytochrome P450, family 71, subfamily B, polypeptide 26 )

HSP 1 Score: 484.2 bits (1245), Expect = 1.3e-136
Identity = 240/459 (52.29%), Postives = 331/459 (72.11%), Query Frame = 0

Query: 25  LLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRP 84
           ++G+LH +G + H+SLWKLS+KYG VMLLKLG +PT+++SS+  A++ L+ +DL CCSRP
Sbjct: 40  IIGNLHQLGELQHQSLWKLSKKYGPVMLLKLGKVPTLILSSSETAKQALRDYDLHCCSRP 99

Query: 85  RLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQSFQLIREEEVGLLLKSLF 144
            LA     SYN LD+  SPY+++W+E+RK+   ELFSA ++QS Q I++EEV  ++ S+ 
Sbjct: 100 SLAGGRELSYNNLDMSSSPYNEYWKELRKLCSQELFSANKIQSIQPIKDEEVKKVIDSIA 159

Query: 145 ESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNFHEVIRRAIAALASFSVTD 204
           ESSS   PV+LS+    LT +++ + AFG SF G  L++  F++++R     L SFS +D
Sbjct: 160 ESSSLKNPVNLSKTFLALTTSVVCKAAFGVSFEGSVLNSDRFNKLVRDTFEMLGSFSASD 219

Query: 205 FFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMATSENENIIDVLLRMERER 264
           F P VGWIID+ +G+ G  +KSF +LDAF + + D          +E+++DVLLR+E+E 
Sbjct: 220 FIPYVGWIIDKFNGLQGWRKKSFRDLDAFYEQIFDLHKEEKEVG-SEDLVDVLLRLEKEE 279

Query: 265 SESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRNPRVMKKLQYEIRSCIK-E 324
                 +LTR+ IKAI+M+I + G++  A T+ WAMAEL +NPRVMKK+Q EIR+ IK +
Sbjct: 280 IVVGNGKLTRNHIKAILMNILLGGIDTSAITMTWAMAELAKNPRVMKKVQAEIRNQIKNK 339

Query: 325 DQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLNGYDIAPMVHLHVNVWAI 384
           +++   D +KL+YL+MV+KE  RLH P PLLLPR+ ++ F++NGY I     LHVNVWAI
Sbjct: 340 ERISFDDTDKLEYLKMVIKETWRLHPPTPLLLPRDVITEFEINGYTIPAKTRLHVNVWAI 399

Query: 385 GRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCPGVNMGAITVELILANLL 444
           GRDPD+W +PE FLPERF +S +D +GQNFELL FG+GRR CPG+ MG   VE  LAN+L
Sbjct: 400 GRDPDTWKDPEMFLPERFNDSNIDAKGQNFELLSFGSGRRICPGLYMGTTMVEFGLANML 459

Query: 445 LCFDWKLPDGM-KEDVDMEEDAGLTVAKKSPLKLVPVCY 482
             FDWKLP+GM  ED+DMEE  GLTV+KKS L LVPV Y
Sbjct: 460 YHFDWKLPEGMVVEDIDMEEAPGLTVSKKSELVLVPVKY 497

BLAST of Moc09g41000 vs. TAIR 10
Match: AT2G02580.1 (cytochrome P450, family 71, subfamily B, polypeptide 9 )

HSP 1 Score: 483.8 bits (1244), Expect = 1.6e-136
Identity = 243/463 (52.48%), Postives = 327/463 (70.63%), Query Frame = 0

Query: 25  LLGHLHLIGSIPHRSLWKLSRKYGSVMLLKLGSIPTIVISSAAAAREVLQVHDLACCSRP 84
           ++G+LH +G +PH+SLW LS+ YG VMLLKLGS+PT+V+SS+  A++VL+++DL CCSRP
Sbjct: 40  IIGNLHQLGELPHQSLWSLSKTYGPVMLLKLGSVPTVVVSSSETAKQVLKINDLHCCSRP 99

Query: 85  RLAASARFSYNFLDIGLSPYSDHWREVRKISVLELFSARRVQSFQLIREEEVGLLLKSLF 144
            LA +   SYN+LDI  SP+ D+W+E+R+I V ELFSA+RV S Q I+EEEV  L+ S  
Sbjct: 100 SLAGAKELSYNYLDIAFSPFDDYWKELRRICVQELFSAKRVHSIQPIKEEEVRKLIVSAT 159

Query: 145 ESSSSAAPVDLSEKSYTLTANIMTRIAFGKSFRGGELDNGNFHEVIRRAIAALASFSVTD 204
           ES+S  +PV+LSEK   LT +++ + AF   F    L+N  F ++I  A   L SFS ++
Sbjct: 160 ESASQKSPVNLSEKFLDLTVSVICKAAFSLDFHTSVLNNDGFDKLIHDAFLFLGSFSASN 219

Query: 205 FFPTVGWIIDRVSGVHGRLEKSFAELDAFLQHVVDDRINFMATSEN----ENIIDVLLRM 264
           FFP  GWIID ++G+  R EKS  +LD F Q + D     +   EN    E+ +D+LL++
Sbjct: 220 FFPNGGWIIDWLTGLQRRREKSVKDLDVFYQQMFD-----LHKQENKQGVEDFVDLLLKL 279

Query: 265 ERERSESDALQLTRDCIKAIIMDIFIAGVNPGAGTIIWAMAELIRNPRVMKKLQYEIRS- 324
           E+E +     +LTR+ +KAI+M++ +  +N  A T+ WAMAELIRNPRVMKK+Q EIR+ 
Sbjct: 280 EKEETVLGYGKLTRNHVKAILMNVLLGAINTSAMTMTWAMAELIRNPRVMKKVQSEIRNQ 339

Query: 325 CIKEDQVKESDLEKLQYLRMVVKEVLRLHAPAPLLLPRETMSHFKLNGYDIAPMVHLHVN 384
            I +  +   D++ L YL+MV+KE  RLH P PLLLPRE MS F++NGY I P   L+VN
Sbjct: 340 MINKSVITLDDIDHLPYLKMVIKETWRLHPPVPLLLPREVMSEFEINGYKIQPKTLLYVN 399

Query: 385 VWAIGRDPDSWSNPEEFLPERFMESCVDFRGQNFELLPFGAGRRSCPGVNMGAITVELIL 444
           VWAIGRDPDSW + + F PERFM++ +D +GQNFELLPFG+GRR CPG+ MG   VE  L
Sbjct: 400 VWAIGRDPDSWKDADMFYPERFMDNNIDAKGQNFELLPFGSGRRICPGMYMGTTMVEFGL 459

Query: 445 ANLLLCFDWKLPDGM-KEDVDMEEDAGLTVAKKSPLKLVPVCY 482
           AN+L  FDW++PDGM  ED+DMEE  GL V KK+ L LVPV Y
Sbjct: 460 ANMLYQFDWEVPDGMVVEDIDMEESPGLAVGKKNELLLVPVKY 497

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151972.12.1e-282100.00cytochrome P450 71B34-like isoform X1 [Momordica charantia] >XP_022151976.1 cyto... [more]
XP_022151974.18.1e-279100.00cytochrome P450 71B34-like isoform X2 [Momordica charantia] >XP_022151975.1 cyto... [more]
XP_023541440.11.7e-22079.88cytochrome P450 71B26-like [Cucurbita pepo subsp. pepo][more]
XP_022945237.11.6e-21879.28cytochrome P450 71B37-like [Cucurbita moschata] >XP_022945238.1 cytochrome P450 ... [more]
XP_038892117.12.0e-20875.49cytochrome P450 71B26-like [Benincasa hispida] >XP_038892118.1 cytochrome P450 7... [more]
Match NameE-valueIdentityDescription
Q9LIP64.2e-13752.52Cytochrome P450 71B34 OS=Arabidopsis thaliana OX=3702 GN=CYP71B34 PE=2 SV=1[more]
Q9LIP51.4e-13552.30Cytochrome P450 71B35 OS=Arabidopsis thaliana OX=3702 GN=CYP71B35 PE=2 SV=1[more]
Q9LIP41.4e-13551.74Cytochrome P450 71B36 OS=Arabidopsis thaliana OX=3702 GN=CYP71B36 PE=3 SV=1[more]
Q9LTL01.8e-13552.29Cytochrome P450 71B26 OS=Arabidopsis thaliana OX=3702 GN=CYP71B26 PE=2 SV=1[more]
O647182.3e-13552.48Cytochrome P450 71B9 OS=Arabidopsis thaliana OX=3702 GN=CYP71B9 PE=2 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1DEX71.0e-282100.00cytochrome P450 71B34-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A6J1DCP03.9e-279100.00cytochrome P450 71B34-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A6J1G0947.8e-21979.28cytochrome P450 71B37-like OS=Cucurbita moschata OX=3662 GN=LOC111449540 PE=3 SV... [more]
A0A6J1GYQ41.3e-19773.68cytochrome P450 71B34-like OS=Cucurbita moschata OX=3662 GN=LOC111458729 PE=3 SV... [more]
A0A6J1DDC02.2e-19770.53cytochrome P450 71B34-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
Match NameE-valueIdentityDescription
AT3G26300.13.0e-13852.52cytochrome P450, family 71, subfamily B, polypeptide 34 [more]
AT3G26310.19.7e-13752.30cytochrome P450, family 71, subfamily B, polypeptide 35 [more]
AT3G26320.19.7e-13751.74cytochrome P450, family 71, subfamily B, polypeptide 36 [more]
AT3G26290.11.3e-13652.29cytochrome P450, family 71, subfamily B, polypeptide 26 [more]
AT2G02580.11.6e-13652.48cytochrome P450, family 71, subfamily B, polypeptide 9 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 162..180
score: 26.01
coord: 379..403
score: 39.04
coord: 415..425
score: 59.17
coord: 296..322
score: 31.54
coord: 338..356
score: 35.99
coord: 425..448
score: 29.3
coord: 44..63
score: 36.69
coord: 68..89
score: 24.87
coord: 276..293
score: 25.53
IPR001128Cytochrome P450PRINTSPR00385P450coord: 416..425
score: 62.69
coord: 287..304
score: 27.08
coord: 339..350
score: 39.09
IPR001128Cytochrome P450PFAMPF00067p450coord: 24..469
e-value: 1.7E-85
score: 287.5
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 18..478
e-value: 2.1E-112
score: 378.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 24..478
NoneNo IPR availablePANTHERPTHR47956CYTOCHROME P450 71B11-RELATEDcoord: 25..481
NoneNo IPR availablePANTHERPTHR47956:SF5CYTOCHROME P450 71B16-RELATEDcoord: 25..481
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 418..427

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc09g41000.1Moc09g41000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen