Moc09g36200 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g36200
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 family protein
Location	chr9: 27870264 .. 27872377 (+)
RNA-Seq Expression	Moc09g36200
Synteny	Moc09g36200

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAACGTCTAGAGGTATGCTTGCTATAAAACCTACTATAACAACTTTAGAAGCTACGGTACCAAGTTCTAGCGTCGAAGTGTCTTCTTCAACCATGTTTTTTGAAGCACTTGCACCATCCACCGTTCCTACTCCTCTAGCCATATCCAATAATGAAGCGCTTACTTTGACAACTTCTAAAGGCACAGTTGGTGTAGTAGTACCTACTCCATCTACATCCAAGGTCACTTTCACGGTAGGACGTAGCCCGTTGATTTCATTGTCTCCACGTTCTGATGACTTCGTCAATTTTGAGGTTCCAAATGGAATGGGCGCAAGGCCACCAATGACTGATTGGGCTATTCGAAGCTGCAAGCCCAAAGGAAAATTTAGGTATTCTGCTCCCAGTGATCGCAGAAATTAGTAGTTGATTCTTGTATGACATGTCATACACAAAACCAAACTCAAAAACATAATCAACCTTACACATTATTAATTAGTTTCTTATTAATTATTATCTTCTCTCTTATACTTGTACCAATTCTCTTAAAGTTTCTATGTATCATCAAAAGTTCAGTTACACTACACAAATAATTACTCTGGCTAACTAAGAGCGGATTAGCTTAAATGAATATATATAATTAGTCTATTTAGTTTTTGGTTTAACAAATTCTAAATTGAATGAATCGATTTGGTCATCTTTTTAAAAACAAAACTGAAATGACATTGATTGTTGATTGATTTTGATAAGTTTTGATGTGCACCCCTACCCCAAACTTATTTTTATTGAATTATTTGCGGGGTTATAGATTAAAGCTAATTATATAAACTAGAATTAGATAAATAATATTACAAATATCAAAGTTACCAAACTTGTTAAATATGATAATCTAAGTACAGTTCAACAGTTAAGGTATCAATAACTTTTCTTTTCAGTTGAACGTTCGAATTATGACAACATACATATAAAAATAATGATATTTTCAAGTCATACATAACATACAACTTACATGATCATATTAATAAATAGTGAATATATAGTTAGTTAATTGAAGAGATAATTAATCAGCCAAGATGAGTATAGTTCAGAGGTGATTGACATCTATCTAGATCAAAAGTTTGAATTCATACCTCCAATTTGTTGTATTAAAAGGATTTAACGATGCTATAATTAAATAAAGGAATCATACCAAAATTTTGAATTATTTACAAAGTAAATTTATCCATCTCCCGCAGCTTTAATTTAAGAAATAATTGCACTATATAATTTTCCAATTAATCCGTAGGCGTAAATTATACTCTTTCCTTGTTTAATTTTAACAAAATCGATAATATACTTCATAGATTTTTCATATCACAAAAATAAAGTTAAATTTGAATAAAAGATATAATTATATCTTCACTAATCTGATATTTTCCATATATTTAAACTAGTTAGGAGACGGAAAACGATTTCGCATAAAAAAGGGAATTGAAATTCGGAGAAGAATTAGGAAAGAAATCCCCAGACCTAAACCCGCTGCCATTAAATACCTTCCTTTAATCTCTGATCGTCTCCAAAGAATACCTAAATTCTCTCTGTAGAATCTACATTACTCTCTCTCGCTTCTTCAATTTCCTTTCGCCGATCGATCGCGTTTTCTCTTGTTGTTGAATCGATTAAGTTGCGCGATGAGGAGGATTCTGCTGAAGACTTGTGATAACCAGATTTACGAAGTGGAGGAGGCGATCGCGAAGCAATCCGGAGTGATTCGTAACTTTCTGGAAGATTTCGATGGCGATTGCGTCGAGATTCCGCTGCTGAAAGTGAACGAACGAATGCTGAAGGTGGTAATGGAGTGGTGCGCGATGCACGCCGGGGAGGAATTGGAGGAGAAGGAACTCCAAGATTGGGAGAGCAAGTTCGTGGCGATGGACGATCTTGATTTTCTTTACGAGGTACTGATGGCGGCGAATTACCTAGAAGTGGTGGATTTGGTCCATCGCCTTTGCAAGAAAGTTGCCGACATCATCGCCGGAAAGCAGCCGGAGGAAATTCGGAAGATCTTTAACATTGAGAATGATTTCACGCCGGAAGAGGAGGCGGAGCTCCGGAAGCGGAATGCTTGGACTCTGGATCCCAAAAACAAGTAG

mRNA sequence

ATGGCAACGTCTAGAGGTATGCTTGCTATAAAACCTACTATAACAACTTTAGAAGCTACGGTACCAAGTTCTAGCGTCGAAGTGTCTTCTTCAACCATGTTTTTTGAAGCACTTGCACCATCCACCGTTCCTACTCCTCTAGCCATATCCAATAATGAAGCGCTTACTTTGACAACTTCTAAAGGCACAGTTGGTGTAGTAGTACCTACTCCATCTACATCCAAGGTCACTTTCACGGTAGGACGTAGCCCGTTGATTTCATTGTCTCCACGTTCTGATGACTTCGTCAATTTTGAGGTTCCAAATGGAATGGGCGCAAGGCCACCAATGACTGATTGGGCTATTCGAAGCTGCAAGCCCAAAGGAAAATTTAGTTGCGCGATGAGGAGGATTCTGCTGAAGACTTGTGATAACCAGATTTACGAAGTGGAGGAGGCGATCGCGAAGCAATCCGGAGTGATTCGTAACTTTCTGGAAGATTTCGATGGCGATTGCGTCGAGATTCCGCTGCTGAAAGTGAACGAACGAATGCTGAAGGTGGTAATGGAGTGGTGCGCGATGCACGCCGGGGAGGAATTGGAGGAGAAGGAACTCCAAGATTGGGAGAGCAAGTTCGTGGCGATGGACGATCTTGATTTTCTTTACGAGGTACTGATGGCGGCGAATTACCTAGAAGTGGTGGATTTGGTCCATCGCCTTTGCAAGAAAGTTGCCGACATCATCGCCGGAAAGCAGCCGGAGGAAATTCGGAAGATCTTTAACATTGAGAATGATTTCACGCCGGAAGAGGAGGCGGAGCTCCGGAAGCGGAATGCTTGGACTCTGGATCCCAAAAACAAGTAG

Coding sequence (CDS)

ATGGCAACGTCTAGAGGTATGCTTGCTATAAAACCTACTATAACAACTTTAGAAGCTACGGTACCAAGTTCTAGCGTCGAAGTGTCTTCTTCAACCATGTTTTTTGAAGCACTTGCACCATCCACCGTTCCTACTCCTCTAGCCATATCCAATAATGAAGCGCTTACTTTGACAACTTCTAAAGGCACAGTTGGTGTAGTAGTACCTACTCCATCTACATCCAAGGTCACTTTCACGGTAGGACGTAGCCCGTTGATTTCATTGTCTCCACGTTCTGATGACTTCGTCAATTTTGAGGTTCCAAATGGAATGGGCGCAAGGCCACCAATGACTGATTGGGCTATTCGAAGCTGCAAGCCCAAAGGAAAATTTAGTTGCGCGATGAGGAGGATTCTGCTGAAGACTTGTGATAACCAGATTTACGAAGTGGAGGAGGCGATCGCGAAGCAATCCGGAGTGATTCGTAACTTTCTGGAAGATTTCGATGGCGATTGCGTCGAGATTCCGCTGCTGAAAGTGAACGAACGAATGCTGAAGGTGGTAATGGAGTGGTGCGCGATGCACGCCGGGGAGGAATTGGAGGAGAAGGAACTCCAAGATTGGGAGAGCAAGTTCGTGGCGATGGACGATCTTGATTTTCTTTACGAGGTACTGATGGCGGCGAATTACCTAGAAGTGGTGGATTTGGTCCATCGCCTTTGCAAGAAAGTTGCCGACATCATCGCCGGAAAGCAGCCGGAGGAAATTCGGAAGATCTTTAACATTGAGAATGATTTCACGCCGGAAGAGGAGGCGGAGCTCCGGAAGCGGAATGCTTGGACTCTGGATCCCAAAAACAAGTAG

Protein sequence

MATSRGMLAIKPTITTLEATVPSSSVEVSSSTMFFEALAPSTVPTPLAISNNEALTLTTSKGTVGVVVPTPSTSKVTFTVGRSPLISLSPRSDDFVNFEVPNGMGARPPMTDWAIRSCKPKGKFSCAMRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAMHAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPEEIRKIFNIENDFTPEEEAELRKRNAWTLDPKNK

Homology

[Close]

BLAST of Moc09g36200 vs. NCBI nr
Match: XP_022139312.1 (SKP1-like protein 14 [Momordica charantia])

HSP 1 Score: 309.3 bits (791), Expect = 3.4e-80
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 0


Query: 128 MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAM 187
           MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAM
Sbjct: 1   MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAM 60

Query: 188 HAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPE 247
           HAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPE
Sbjct: 61  HAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPE 120

Query: 248 EIRKIFNIENDFTPEEEAELRKRNAWTLDPKNK 281
           EIRKIFNIENDFTPEEEAELRKRNAWTLDPKNK
Sbjct: 121 EIRKIFNIENDFTPEEEAELRKRNAWTLDPKNK 153

[Close]

BLAST of Moc09g36200 vs. NCBI nr
Match: KAE8648853.1 (hypothetical protein Csa_008932 [Cucumis sativus])

HSP 1 Score: 202.6 bits (514), Expect = 4.5e-48
Identity = 114/251 (45.42%), Postives = 158/251 (62.95%), Query Frame = 0


Query: 33  MFFEALAPSTVPTPLAISNNEALTLTTSKGTVGV-VVPTPSTSKVTFTVGRSPLISLS-- 92
           +FF  L  +   +   ++ + A+       T+     P P+ S+ T   G    +S S  
Sbjct: 9   LFFFLLVLALASSSTTMATSRAMLFIEPTPTIDAKPSPPPTVSEATLVAGFGSSLSTSMP 68

Query: 93  PRSDDFVNFEVPNGMG-ARPPMTDWAIRSCKPKGKFSCAMRRILLKTCDNQIYEVEEAIA 152
           P   DFV FEVP+ +  A PPM DW IRSCKPKG+         +++ +N+I++V E +A
Sbjct: 69  PSFGDFVAFEVPSEVSTANPPMPDWMIRSCKPKGR---------MRSSENEIFKVSEEVA 128

Query: 153 KQSGVIRNFLE--DFDGDCVEIPLLKVNERMLKVVMEWCAMHAGEELEEKELQDWESKFV 212
           KQS V+R FLE  D + D + IPL  ++ R+L +V+EW  MH  E+L E+ L  W++KF+
Sbjct: 129 KQSVVVRIFLEEDDSNNDEITIPLPNISGRLLGMVIEWIVMHVEEKLAEEALHAWKTKFL 188

Query: 213 AMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPEEIRKIFNIENDFTPEEEAE 272
              DLD L+E++MAANYLEV DL H  C+ VAD I+GK PEEIRKIFNI NDFTPEEEAE
Sbjct: 189 EDLDLDLLFELIMAANYLEVTDLFHATCQCVADKISGKSPEEIRKIFNITNDFTPEEEAE 248

Query: 273 LRKRNAWTLDP 278
           +R++NAW  +P
Sbjct: 249 IRRQNAWVFEP 250

[Close]

BLAST of Moc09g36200 vs. NCBI nr
Match: TYK09255.1 (SKP1-like protein 12 [Cucumis melo var. makuwa])

HSP 1 Score: 174.1 bits (440), Expect = 1.7e-39
Identity = 84/152 (55.26%), Postives = 117/152 (76.97%), Query Frame = 0


Query: 128 MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLED--FDGDCVEIPLLKVNERMLKVVMEWC 187
           MR + L++ +N+I++V E +AKQS V+RNFLE+   +GD + IPL  ++ ++L +V+EW 
Sbjct: 1   MRTVNLRSSENEIFKVSEEVAKQSVVVRNFLEEDNSNGDEITIPLPNISGKLLGMVIEWI 60

Query: 188 AMHAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQ 247
           AMH  E+L E++L  W++KF+   DLD L+E++MAANYLEV DL H  C+ VAD I+GK 
Sbjct: 61  AMHVEEKLAEEDLHAWKTKFMEDLDLDLLFELIMAANYLEVTDLFHATCQCVADKISGKS 120

Query: 248 PEEIRKIFNIENDFTPEEEAELRKRNAWTLDP 278
           PEEIRKIFNI NDFTPEEEAE+R++NAW  +P
Sbjct: 121 PEEIRKIFNITNDFTPEEEAEIRRQNAWVFEP 152

[Close]

BLAST of Moc09g36200 vs. NCBI nr
Match: XP_008446842.1 (PREDICTED: SKP1-like protein 12 [Cucumis melo])

HSP 1 Score: 174.1 bits (440), Expect = 1.7e-39
Identity = 84/152 (55.26%), Postives = 117/152 (76.97%), Query Frame = 0


Query: 128 MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLED--FDGDCVEIPLLKVNERMLKVVMEWC 187
           MR + L++ +N+I++V E +AKQS V+RNFLE+   +GD + IPL  ++ ++L +V+EW 
Sbjct: 1   MRTVNLRSSENEIFKVSEEVAKQSVVVRNFLEEDNSNGDEITIPLPNISGKLLGMVIEWI 60

Query: 188 AMHAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQ 247
           AMH  E+L E++L  W++KF+   DLD L+E++MAANYLEV DL H  C+ VAD I+GK 
Sbjct: 61  AMHVEEKLAEEDLHAWKTKFMEDLDLDLLFELIMAANYLEVTDLFHATCQCVADKISGKS 120

Query: 248 PEEIRKIFNIENDFTPEEEAELRKRNAWTLDP 278
           PEEIRKIFNI NDFTPEEEAE+R++NAW  +P
Sbjct: 121 PEEIRKIFNITNDFTPEEEAEIRRQNAWVFEP 152

[Close]

BLAST of Moc09g36200 vs. NCBI nr
Match: KAA0034704.1 (SKP1-like protein 12 [Cucumis melo var. makuwa])

HSP 1 Score: 174.1 bits (440), Expect = 1.7e-39
Identity = 84/152 (55.26%), Postives = 117/152 (76.97%), Query Frame = 0


Query: 128 MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLED--FDGDCVEIPLLKVNERMLKVVMEWC 187
           MR + L++ +N+I++V E +AKQS V+RNFLE+   +GD + IPL  ++ ++L +V+EW 
Sbjct: 1   MRTVNLRSSENEIFKVSEEVAKQSVVVRNFLEEDNSNGDEITIPLPNISGKLLGMVIEWI 60

Query: 188 AMHAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQ 247
           AMH  E+L E++L  W++KF+   DLD L+E++MAANYLEV DL H  C+ VAD I+GK 
Sbjct: 61  AMHVEEKLAEEDLHAWKTKFMEDLDLDLLFELIMAANYLEVTDLFHATCQCVADKISGKS 120

Query: 248 PEEIRKIFNIENDFTPEEEAELRKRNAWTLDP 278
           PEEIRKIFNI NDFTPEEEAE+R++NAW  +P
Sbjct: 121 PEEIRKIFNITNDFTPEEEAEIRRQNAWVFEP 152

[Close]

BLAST of Moc09g36200 vs. ExPASy Swiss-Prot
Match: B8MDP8 (E3 ubiquitin ligase complex SCF subunit sconC OS=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) OX=441959 GN=sconC PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 6.3e-29
Identity = 67/157 (42.68%), Postives = 101/157 (64.33%), Query Frame = 0


Query: 130 RILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAMH- 189
           ++ L++ D     VE A+A++S +I+N LED       +P+  VNE +LK V+EWC  H 
Sbjct: 4   QVTLQSSDQVNITVERAVAERSMLIKNLLEDLGESEEPVPIPNVNESVLKKVIEWCTHHK 63

Query: 190 -----AGEELEEK----ELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVAD 249
                 GEE + +    E+ +W+ KF+ +D  + L+E+++AANYL++  L+   CK VA+
Sbjct: 64  NDPQTTGEEDDNRRRTTEIDEWDQKFMQVDQ-EMLFEIILAANYLDIKALLDVGCKTVAN 123

Query: 250 IIAGKQPEEIRKIFNIENDFTPEEEAELRKRNAWTLD 277
           +I GK PEEIRK FNI+NDFTPEEE ++R+ N W  D
Sbjct: 124 MIKGKSPEEIRKTFNIQNDFTPEEEDQIRRENEWAED 159

[Close]

BLAST of Moc09g36200 vs. ExPASy Swiss-Prot
Match: B6QGB9 (E3 ubiquitin ligase complex SCF subunit sconC OS=Talaromyces marneffei (strain ATCC 18224 / CBS 334.59 / QM 7333) OX=441960 GN=sconC PE=3 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.1e-28
Identity = 66/157 (42.04%), Postives = 102/157 (64.97%), Query Frame = 0


Query: 130 RILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAMH- 189
           ++ L++ D+    VE A+A++S +I+N LED       +P+  VNE +LK V+EWC  H 
Sbjct: 4   QVTLQSSDSVDITVERAVAERSMLIKNLLEDLGESEEPVPIPNVNESVLKKVIEWCTHHK 63

Query: 190 -----AGEELEEK----ELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVAD 249
                 GE+ + +    E+ +W+ KF+ +D  + L+E+++AANYL++  L+   CK VA+
Sbjct: 64  NDPQSTGEDDDNRRRTTEIDEWDQKFMQVDQ-EMLFEIILAANYLDIKALLDVGCKTVAN 123

Query: 250 IIAGKQPEEIRKIFNIENDFTPEEEAELRKRNAWTLD 277
           +I GK PEEIRK FNI+NDFTPEEE ++R+ N W  D
Sbjct: 124 MIKGKSPEEIRKTFNIQNDFTPEEEDQIRRENEWAED 159

[Close]

BLAST of Moc09g36200 vs. ExPASy Swiss-Prot
Match: Q9Y709 (Suppressor of kinetochore protein 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=skp1 PE=1 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.4e-28
Identity = 68/161 (42.24%), Postives = 103/161 (63.98%), Query Frame = 0


Query: 128 MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAM 187
           M +I L + DN+ + V++ IA++S +I+N LED     V IPL  V+  +L+ V+EWC  
Sbjct: 1   MSKIKLISSDNEEFVVDQLIAERSMLIKNMLEDVGEINVPIPLPNVSSNVLRKVLEWCEH 60

Query: 188 H-----AGEELE-------EKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCK 247
           H     +G E E         ++ +W+ KF+A+D  + L+E+++A+NYL++  L+   CK
Sbjct: 61  HKNDLYSGTEEESDIRLKKSTDIDEWDRKFMAVDQ-EMLFEIVLASNYLDIKPLLDTGCK 120

Query: 248 KVADIIAGKQPEEIRKIFNIENDFTPEEEAELRKRNAWTLD 277
            VA++I GK PE+IRK FNI NDFTPEEE ++RK N W  D
Sbjct: 121 TVANMIRGKSPEDIRKTFNIPNDFTPEEEEQIRKENEWAED 160

[Close]

BLAST of Moc09g36200 vs. ExPASy Swiss-Prot
Match: O49484 (SKP1-like protein 11 OS=Arabidopsis thaliana OX=3702 GN=ASK11 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 3.1e-28
Identity = 68/153 (44.44%), Postives = 99/153 (64.71%), Query Frame = 0


Query: 129 RRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVE--IPLLKVNERMLKVVMEWCA 188
           + I+L + D Q +EVEEA+A QS  I + +ED   DCV   IPL  V  ++L  V+E+C 
Sbjct: 4   KMIVLMSSDGQSFEVEEAVAIQSQTIAHMVED---DCVADGIPLANVESKILVKVIEYCK 63

Query: 189 MHAGEE---LEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAG 248
            H  +E   + E++L +W+ KF+ ++    ++E+++AANYL +  L+   C+ VAD+I G
Sbjct: 64  KHHVDEANPISEEDLNNWDEKFMDLEQ-STIFELILAANYLNIKSLLDLTCQTVADMIKG 123

Query: 249 KQPEEIRKIFNIENDFTPEEEAELRKRNAWTLD 277
           K PEEIR  FNIENDFTPEEE  +RK N W  +
Sbjct: 124 KTPEEIRSTFNIENDFTPEEEEAVRKENQWAFE 152

[Close]

BLAST of Moc09g36200 vs. ExPASy Swiss-Prot
Match: O81057 (SKP1-like protein 14 OS=Arabidopsis thaliana OX=3702 GN=ASK14 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 3.1e-28
Identity = 65/149 (43.62%), Postives = 99/149 (66.44%), Query Frame = 0


Query: 130 RILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCV--EIPLLKVNERMLKVVMEWCAM 189
           +I+L + D + +EVEEA+A++  ++ + +ED   DCV  E+PL  V  ++L +V+E+C  
Sbjct: 5   KIVLSSSDGESFEVEEAVARKLKIVEHMIED---DCVVTEVPLQNVTGKILSIVVEYCKK 64

Query: 190 HAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPE 249
           H  +E E  E + W+ +F+   D   ++++L+AANYL +  L+    + VAD I  K PE
Sbjct: 65  HVVDE-ESDEFKTWDEEFMKKFDQPTVFQLLLAANYLNIKGLLDLSAQTVADRIKDKTPE 124

Query: 250 EIRKIFNIENDFTPEEEAELRKRNAWTLD 277
           EIR+IFNIENDFTPEEEA +RK NAW  +
Sbjct: 125 EIREIFNIENDFTPEEEAAVRKENAWAFE 149

[Close]

BLAST of Moc09g36200 vs. ExPASy TrEMBL
Match: A0A6J1CDL6 (SKP1-like protein OS=Momordica charantia OX=3673 GN=LOC111010258 PE=3 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.6e-80
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 0


Query: 128 MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAM 187
           MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAM
Sbjct: 1   MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAM 60

Query: 188 HAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPE 247
           HAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPE
Sbjct: 61  HAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPE 120

Query: 248 EIRKIFNIENDFTPEEEAELRKRNAWTLDPKNK 281
           EIRKIFNIENDFTPEEEAELRKRNAWTLDPKNK
Sbjct: 121 EIRKIFNIENDFTPEEEAELRKRNAWTLDPKNK 153

[Close]

BLAST of Moc09g36200 vs. ExPASy TrEMBL
Match: A0A5A7SU15 (SKP1-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007240 PE=3 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 8.2e-40
Identity = 84/152 (55.26%), Postives = 117/152 (76.97%), Query Frame = 0


Query: 128 MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLED--FDGDCVEIPLLKVNERMLKVVMEWC 187
           MR + L++ +N+I++V E +AKQS V+RNFLE+   +GD + IPL  ++ ++L +V+EW 
Sbjct: 1   MRTVNLRSSENEIFKVSEEVAKQSVVVRNFLEEDNSNGDEITIPLPNISGKLLGMVIEWI 60

Query: 188 AMHAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQ 247
           AMH  E+L E++L  W++KF+   DLD L+E++MAANYLEV DL H  C+ VAD I+GK 
Sbjct: 61  AMHVEEKLAEEDLHAWKTKFMEDLDLDLLFELIMAANYLEVTDLFHATCQCVADKISGKS 120

Query: 248 PEEIRKIFNIENDFTPEEEAELRKRNAWTLDP 278
           PEEIRKIFNI NDFTPEEEAE+R++NAW  +P
Sbjct: 121 PEEIRKIFNITNDFTPEEEAEIRRQNAWVFEP 152

[Close]

BLAST of Moc09g36200 vs. ExPASy TrEMBL
Match: A0A5D3CCN6 (SKP1-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002490 PE=3 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 8.2e-40
Identity = 84/152 (55.26%), Postives = 117/152 (76.97%), Query Frame = 0


Query: 128 MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLED--FDGDCVEIPLLKVNERMLKVVMEWC 187
           MR + L++ +N+I++V E +AKQS V+RNFLE+   +GD + IPL  ++ ++L +V+EW 
Sbjct: 1   MRTVNLRSSENEIFKVSEEVAKQSVVVRNFLEEDNSNGDEITIPLPNISGKLLGMVIEWI 60

Query: 188 AMHAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQ 247
           AMH  E+L E++L  W++KF+   DLD L+E++MAANYLEV DL H  C+ VAD I+GK 
Sbjct: 61  AMHVEEKLAEEDLHAWKTKFMEDLDLDLLFELIMAANYLEVTDLFHATCQCVADKISGKS 120

Query: 248 PEEIRKIFNIENDFTPEEEAELRKRNAWTLDP 278
           PEEIRKIFNI NDFTPEEEAE+R++NAW  +P
Sbjct: 121 PEEIRKIFNITNDFTPEEEAEIRRQNAWVFEP 152

[Close]

BLAST of Moc09g36200 vs. ExPASy TrEMBL
Match: A0A1S3BGP6 (SKP1-like protein OS=Cucumis melo OX=3656 GN=LOC103489446 PE=3 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 8.2e-40
Identity = 84/152 (55.26%), Postives = 117/152 (76.97%), Query Frame = 0


Query: 128 MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLED--FDGDCVEIPLLKVNERMLKVVMEWC 187
           MR + L++ +N+I++V E +AKQS V+RNFLE+   +GD + IPL  ++ ++L +V+EW 
Sbjct: 1   MRTVNLRSSENEIFKVSEEVAKQSVVVRNFLEEDNSNGDEITIPLPNISGKLLGMVIEWI 60

Query: 188 AMHAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQ 247
           AMH  E+L E++L  W++KF+   DLD L+E++MAANYLEV DL H  C+ VAD I+GK 
Sbjct: 61  AMHVEEKLAEEDLHAWKTKFMEDLDLDLLFELIMAANYLEVTDLFHATCQCVADKISGKS 120

Query: 248 PEEIRKIFNIENDFTPEEEAELRKRNAWTLDP 278
           PEEIRKIFNI NDFTPEEEAE+R++NAW  +P
Sbjct: 121 PEEIRKIFNITNDFTPEEEAEIRRQNAWVFEP 152

[Close]

BLAST of Moc09g36200 vs. ExPASy TrEMBL
Match: A0A6J1H051 (SKP1-like protein OS=Cucurbita moschata OX=3662 GN=LOC111458404 PE=3 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 1.2e-38
Identity = 83/152 (54.61%), Postives = 116/152 (76.32%), Query Frame = 0


Query: 128 MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDF--DGDCVEIPLLKVNERMLKVVMEWC 187
           MR   LK+ DN+I++V E IA QS VI++FLEDF  D D + IPL  V+ R+L++V+EW 
Sbjct: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60

Query: 188 AMHAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQ 247
             HA +EL ++E+QDWE+KF+   +++   E++MAANYLEV+ L+HR C+ +AD I+GK 
Sbjct: 61  VKHADDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIADQISGKS 120

Query: 248 PEEIRKIFNIENDFTPEEEAELRKRNAWTLDP 278
           PEEIR+I NI NDFTPEEEAE+R++N W L+P
Sbjct: 121 PEEIRRILNITNDFTPEEEAEIREQNGWDLNP 152

[Close]

BLAST of Moc09g36200 vs. TAIR 10
Match: AT2G03170.1 (SKP1-like 14 )

HSP 1 Score: 127.1 bits (318), Expect = 2.2e-29
Identity = 65/149 (43.62%), Postives = 99/149 (66.44%), Query Frame = 0


Query: 130 RILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCV--EIPLLKVNERMLKVVMEWCAM 189
           +I+L + D + +EVEEA+A++  ++ + +ED   DCV  E+PL  V  ++L +V+E+C  
Sbjct: 5   KIVLSSSDGESFEVEEAVARKLKIVEHMIED---DCVVTEVPLQNVTGKILSIVVEYCKK 64

Query: 190 HAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPE 249
           H  +E E  E + W+ +F+   D   ++++L+AANYL +  L+    + VAD I  K PE
Sbjct: 65  HVVDE-ESDEFKTWDEEFMKKFDQPTVFQLLLAANYLNIKGLLDLSAQTVADRIKDKTPE 124

Query: 250 EIRKIFNIENDFTPEEEAELRKRNAWTLD 277
           EIR+IFNIENDFTPEEEA +RK NAW  +
Sbjct: 125 EIREIFNIENDFTPEEEAAVRKENAWAFE 149

[Close]

BLAST of Moc09g36200 vs. TAIR 10
Match: AT4G34210.1 (SKP1-like 11 )

HSP 1 Score: 127.1 bits (318), Expect = 2.2e-29
Identity = 68/153 (44.44%), Postives = 99/153 (64.71%), Query Frame = 0


Query: 129 RRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVE--IPLLKVNERMLKVVMEWCA 188
           + I+L + D Q +EVEEA+A QS  I + +ED   DCV   IPL  V  ++L  V+E+C 
Sbjct: 4   KMIVLMSSDGQSFEVEEAVAIQSQTIAHMVED---DCVADGIPLANVESKILVKVIEYCK 63

Query: 189 MHAGEE---LEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAG 248
            H  +E   + E++L +W+ KF+ ++    ++E+++AANYL +  L+   C+ VAD+I G
Sbjct: 64  KHHVDEANPISEEDLNNWDEKFMDLEQ-STIFELILAANYLNIKSLLDLTCQTVADMIKG 123

Query: 249 KQPEEIRKIFNIENDFTPEEEAELRKRNAWTLD 277
           K PEEIR  FNIENDFTPEEE  +RK N W  +
Sbjct: 124 KTPEEIRSTFNIENDFTPEEEEAVRKENQWAFE 152

[Close]

BLAST of Moc09g36200 vs. TAIR 10
Match: AT4G34470.1 (SKP1-like 12 )

HSP 1 Score: 123.2 bits (308), Expect = 3.2e-28
Identity = 67/153 (43.79%), Postives = 97/153 (63.40%), Query Frame = 0


Query: 129 RRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVE--IPLLKVNERMLKVVMEWCA 188
           + I+L + D Q +EVEEA+A QS  I + +ED   DCV   IPL  V  ++L  V+E+C 
Sbjct: 4   KMIVLMSSDGQSFEVEEAVAIQSQTIAHMVED---DCVADGIPLANVESKILVKVIEYCK 63

Query: 189 MHAGEE---LEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAG 248
            +  +E   + E++L  W+ KF+ ++    ++E+++AANYL +  L    C+ VAD+I G
Sbjct: 64  KYHVDEANPISEEDLNKWDEKFMDLEQ-STIFELILAANYLNIKSLFDLTCQTVADMIKG 123

Query: 249 KQPEEIRKIFNIENDFTPEEEAELRKRNAWTLD 277
           K PEEIR  FNIENDFTPEEE  +RK N W  +
Sbjct: 124 KTPEEIRSTFNIENDFTPEEEEAVRKENQWAFE 152

[Close]

BLAST of Moc09g36200 vs. TAIR 10
Match: AT1G75950.1 (S phase kinase-associated protein 1 )

HSP 1 Score: 122.9 bits (307), Expect = 4.2e-28
Identity = 65/161 (40.37%), Postives = 101/161 (62.73%), Query Frame = 0


Query: 129 RRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVE--IPLLKVNERMLKVVMEWCA 188
           ++I+LK+ D + +EVEEA+A +S  I + +ED   DCV+  +PL  V  ++L  V+E+C 
Sbjct: 4   KKIVLKSSDGESFEVEEAVALESQTIAHMVED---DCVDNGVPLPNVTSKILAKVIEYCK 63

Query: 189 MHA-----------GEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCK 248
            H            G    + +L+ W++ F+ +D    L+E+++AANYL + +L+   C+
Sbjct: 64  RHVEAAASKAEAVEGAATSDDDLKAWDADFMKIDQAT-LFELILAANYLNIKNLLDLTCQ 123

Query: 249 KVADIIAGKQPEEIRKIFNIENDFTPEEEAELRKRNAWTLD 277
            VAD+I GK PEEIR  FNI+NDFTPEEE E+R+ N W  +
Sbjct: 124 TVADMIKGKTPEEIRTTFNIKNDFTPEEEEEVRRENQWAFE 160

[Close]

BLAST of Moc09g36200 vs. TAIR 10
Match: AT5G42190.1 (E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 family protein )

HSP 1 Score: 122.1 bits (305), Expect = 7.1e-28
Identity = 67/172 (38.95%), Postives = 106/172 (61.63%), Query Frame = 0


Query: 128 MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVE--IPLLKVNERMLKVVMEWC 187
           +R+I LK+ D + +E++EA+A +S  I++ +ED   DC +  IPL  V  ++L  V+E+C
Sbjct: 4   VRKITLKSSDGENFEIDEAVALESQTIKHMIED---DCTDNGIPLPNVTSKILSKVIEYC 63

Query: 188 AMH--AGEELE-------------------EKELQDWESKFVAMDDLDFLYEVLMAANYL 247
             H  A E+ E                   +++L+ W+S+F+ +D    L+++++AANYL
Sbjct: 64  KRHVEAAEKSETTADAAAATTTTTVASGSSDEDLKTWDSEFIKVDQ-GTLFDLILAANYL 123

Query: 248 EVVDLVHRLCKKVADIIAGKQPEEIRKIFNIENDFTPEEEAELRKRNAWTLD 277
            +  L+   C+ VAD+I GK PEEIRK FNI+NDFTPEEE E+R+ N W  +
Sbjct: 124 NIKGLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 171

The following BLAST results are available for this feature:

BLAST of Moc09g36200 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139312.1	3.4e-80	100.00	SKP1-like protein 14 [Momordica charantia]	[more]
KAE8648853.1	4.5e-48	45.42	hypothetical protein Csa_008932 [Cucumis sativus]	[more]
TYK09255.1	1.7e-39	55.26	SKP1-like protein 12 [Cucumis melo var. makuwa]	[more]
XP_008446842.1	1.7e-39	55.26	PREDICTED: SKP1-like protein 12 [Cucumis melo]	[more]
KAA0034704.1	1.7e-39	55.26	SKP1-like protein 12 [Cucumis melo var. makuwa]	[more]

BLAST of Moc09g36200 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B8MDP8	6.3e-29	42.68	E3 ubiquitin ligase complex SCF subunit sconC OS=Talaromyces stipitatus (strain ...	[more]
B6QGB9	1.1e-28	42.04	E3 ubiquitin ligase complex SCF subunit sconC OS=Talaromyces marneffei (strain A...	[more]
Q9Y709	2.4e-28	42.24	Suppressor of kinetochore protein 1 OS=Schizosaccharomyces pombe (strain 972 / A...	[more]
O49484	3.1e-28	44.44	SKP1-like protein 11 OS=Arabidopsis thaliana OX=3702 GN=ASK11 PE=1 SV=1	[more]
O81057	3.1e-28	43.62	SKP1-like protein 14 OS=Arabidopsis thaliana OX=3702 GN=ASK14 PE=1 SV=1	[more]

BLAST of Moc09g36200 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CDL6	1.6e-80	100.00	SKP1-like protein OS=Momordica charantia OX=3673 GN=LOC111010258 PE=3 SV=1	[more]
A0A5A7SU15	8.2e-40	55.26	SKP1-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G...	[more]
A0A5D3CCN6	8.2e-40	55.26	SKP1-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475...	[more]
A0A1S3BGP6	8.2e-40	55.26	SKP1-like protein OS=Cucumis melo OX=3656 GN=LOC103489446 PE=3 SV=1	[more]
A0A6J1H051	1.2e-38	54.61	SKP1-like protein OS=Cucurbita moschata OX=3662 GN=LOC111458404 PE=3 SV=1	[more]

BLAST of Moc09g36200 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G03170.1	2.2e-29	43.62	SKP1-like 14	[more]
AT4G34210.1	2.2e-29	44.44	SKP1-like 11	[more]
AT4G34470.1	3.2e-28	43.79	SKP1-like 12	[more]
AT1G75950.1	4.2e-28	40.37	S phase kinase-associated protein 1	[more]
AT5G42190.1	7.1e-28	38.95	E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001232	S-phase kinase-associated protein 1-like	SMART	SM00512	skp1_3	coord: 128..226 e-value: 1.7E-21 score: 87.4
IPR016072	SKP1 component, dimerisation	PFAM	PF01466	Skp1	coord: 233..273 e-value: 1.2E-20 score: 73.2
IPR016073	SKP1 component, POZ domain	PFAM	PF03931	Skp1_POZ	coord: 131..188 e-value: 8.4E-15 score: 54.7
IPR011333	SKP1/BTB/POZ domain superfamily	GENE3D	3.30.710.10	Potassium Channel Kv1.1; Chain A	coord: 128..276 e-value: 3.6E-48 score: 165.7
IPR011333	SKP1/BTB/POZ domain superfamily	SUPERFAMILY	54695	POZ domain	coord: 130..191
IPR016897	S-phase kinase-associated protein 1	PANTHER	PTHR11165	SKP1	coord: 130..274
IPR036296	SKP1-like, dimerisation domain superfamily	SUPERFAMILY	81382	Skp1 dimerisation domain-like	coord: 198..273

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc09g36200.1	Moc09g36200.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0031146	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0097602	cullin family protein binding