Moc09g14060 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g14060
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ribonuclease H
Location	chr9: 11983827 .. 11985379 (-)
RNA-Seq Expression	Moc09g14060
Synteny	Moc09g14060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGATTTGAATTCAAGACCTTAAACAATGAGACCAAGTACAAGGATTTTGTCGTAGGACTGAAAGCCAGTCAAGAATATGAAGCAACTTGTCTCCGCATCTACAGTGACTCAATGTTGGTAGTAAACCAAGTTAAAGGAGATTATCAGGTCTAGGGAGTCAAACTAGTTGAATATAAGGCACAGGTATGAAATTTGCTTACTTATTTTGCAGAATGAAAAATTCGACAAATACCACAATCTGAGAACAGAAACGCTGATGCACTAGCACAACTAGCCGCTGCTTATAGAACGAAGCCAAGCATAAGAAAGCTCGAAAGCCTCCTCGTGATGACCGTCCCTCAATTAAGTACCTGGATGAATCCCTTAATGAAATACCTGACAACAGGAAAGTTTCCCAAAAGTAAAATGGAGGCCCAAATACAATGTCGGTCGACACAATATGTTGTTCATAACAGAACCTTGTACAAACGAGGATTCTCACTACCACTCTTGAAGTGTCTTGATCAAGAAAAAACAGCTTATGTCCTACAAGAGGTGCATGAGGGCATTTGTGGTAACCACCTAAGGGCATGATCACTGACACACAAAATTGTCAATCAAGGGTATTATTGGCCAACATTACACCGAGATGTTAAGGACTATACCTGAAAATGCAATCACTGTCAGTGAATTGCCCCTATTCCTAGACAACTAGCAGAGCCATTATCTACCATCACTGGACCCTAGACTTTCACCCAATGGGGAATAGATTTTATCGGTCCCCTCCTGCAGTGGAAAGGACAAACAAAATACGCAGTAGTCGCAGTAGACTACTTCACTAAATGGGCTGAAGCAGAAACACTAGCTACAATCACTAAGCAAAAAATTACCAAGTTTGTATGGCAAAATTTGATATGCCGATTTGGAATCCCCTATGCGATCGTGATAGATAATGGAGGACAATTTAACAATTCGGTTTTCAAAAGCTTATGTGATCAACTAGGCATTCAACACTTTCGATCGTCTCCCACACATTCGCAATCTAATGGTCAGGTGGAAGTCATTAACAAAATCATAAAAAGATGTCTAAAGCTCGTTTGGATTCTTTCAAAGGAAAATGGGCAAAAGAGCTACCAAGCGTACTATTGGCATATCGAACTACAGCGAAAATTGCGACAGGAGAAACCCAATTTTCTTTAGCCTTTGGCACCGAGGCAATGATCCCAGTCGAGATCGGGCTGCCTTCTCCTTGGACAAAAAATTATGATCCCGACACTATAGAAGAATCTTTAATAATCCAGTTGGATTTGTTAGAAGAGTTACAAGAAGGCACTAAGCAACGAATGATGACATACAAGGAACGTAGTGCGCAACATTATAACGTTCGTATGCGACCACGAGCGTTTCAAGTTGGAGATATGGTTCTACGCAAAGTACTCCCTAGCACCAAGAACCCAACAAATGGTGTCCTTTGTCTTACATGGGAAGGTCCGTACGTGGTCATTAAGCTGGCTCAACCAGAAACTTATTACTTGTCTGATGCAATGGGAAAGGTATTGACCCATAAATGA

mRNA sequence

ATGAGATTTGAATTCAAGACCTTAAACAATGAGACCAAGTACAAGGATTTTGTCGTAGGACTGAAAGCCAGTCAAGAATATGAAGCAACTTGTCTCCGCATCTACAGTGACTCAATGTTGGTAGTAAACCAAGTTAAAGGAGATTATCAGTTGGATTTGTTAGAAGAGTTACAAGAAGGCACTAAGCAACGAATGATGACATACAAGGAACGTAGTGCGCAACATTATAACGTTCGTATGCGACCACGAGCGTTTCAAGTTGGAGATATGGTTCTACGCAAAGTACTCCCTAGCACCAAGAACCCAACAAATGGTGTCCTTTGTCTTACATGGGAAGGTCCGTACGTGGTCATTAAGCTGGCTCAACCAGAAACTTATTACTTGTCTGATGCAATGGGAAAGGTATTGACCCATAAATGA

Coding sequence (CDS)

ATGAGATTTGAATTCAAGACCTTAAACAATGAGACCAAGTACAAGGATTTTGTCGTAGGACTGAAAGCCAGTCAAGAATATGAAGCAACTTGTCTCCGCATCTACAGTGACTCAATGTTGGTAGTAAACCAAGTTAAAGGAGATTATCAGTTGGATTTGTTAGAAGAGTTACAAGAAGGCACTAAGCAACGAATGATGACATACAAGGAACGTAGTGCGCAACATTATAACGTTCGTATGCGACCACGAGCGTTTCAAGTTGGAGATATGGTTCTACGCAAAGTACTCCCTAGCACCAAGAACCCAACAAATGGTGTCCTTTGTCTTACATGGGAAGGTCCGTACGTGGTCATTAAGCTGGCTCAACCAGAAACTTATTACTTGTCTGATGCAATGGGAAAGGTATTGACCCATAAATGA

Protein sequence

MRFEFKTLNNETKYKDFVVGLKASQEYEATCLRIYSDSMLVVNQVKGDYQLDLLEELQEGTKQRMMTYKERSAQHYNVRMRPRAFQVGDMVLRKVLPSTKNPTNGVLCLTWEGPYVVIKLAQPETYYLSDAMGKVLTHK

Homology

[Close]

BLAST of Moc09g14060 vs. NCBI nr
Match: XP_030945303.1 (uncharacterized protein LOC115969823 [Quercus lobata])

HSP 1 Score: 102.4 bits (254), Expect = 3.1e-18
Identity = 54/140 (38.57%), Postives = 83/140 (59.29%), Query Frame = 0


Query: 1   MRFEFKTLNNETKYKDFVVGLKASQEYEATCLRIYSDSMLVVNQVKGDY----------- 60
           +  +F   NNE KY+  V GL  ++   AT + +Y DS +V +QV  D+           
Sbjct: 2   VHLDFPITNNEAKYEALVEGLDLAKAMGATNVIVYCDSQVVTSQVNSDFECKGEKMKRYL 61

Query: 61  -QLDLLEELQEGTKQRMMTYKERSAQHYNVRMRPRAFQVGDMVLRKVLPSTKNPTNGVLC 120
            QLDL++E++  T+QR+  Y+   A+HYN R++ R FQV D+VLRKV+ +T++P+ G L 
Sbjct: 62  EQLDLVDEVKATTEQRLARYQNLMAKHYNSRVKHRDFQVRDLVLRKVMGATRDPSQGKLG 121

Query: 121 LTWEGPYVVIKLAQPETYYL 129
             WEGPY +    +  TY+L
Sbjct: 122 PNWEGPYRIASWQRKGTYHL 141

[Close]

BLAST of Moc09g14060 vs. NCBI nr
Match: XP_020417276.1 (uncharacterized protein LOC109948519 [Prunus persica])

HSP 1 Score: 101.7 bits (252), Expect = 5.3e-18
Identity = 58/148 (39.19%), Postives = 85/148 (57.43%), Query Frame = 0


Query: 2   RFEFKTLNNETKYK-----DFVVGLKASQ------EYEATCLRIYSDSMLVVNQVKGDYQ 61
           R  F+T   ET +      + V  ++  Q       Y+AT     +D  L +N       
Sbjct: 820 RTTFRTSTGETPFSLSFGTEAVASVEIGQPTYRTSTYDATA----NDEQLALN------- 879

Query: 62  LDLLEELQEGTKQRMMTYKERSAQHYNVRMRPRAFQVGDMVLRKVLPSTKNPTNGVLCLT 121
           LD ++EL++ +  R + YK+R A++Y+ R++PRAF++GD VLRKV  +TKNP  G L  T
Sbjct: 880 LDFIDELRDQSSMRNIAYKQRIAKYYDSRVKPRAFKMGDWVLRKVSLATKNPNEGTLGPT 939

Query: 122 WEGPYVVIKLAQPETYYLSDAMGKVLTH 139
           WEGPY +IK+ +P TY L D+ GK L H
Sbjct: 940 WEGPYEIIKICRPGTYQLRDSTGKTLPH 956

[Close]

BLAST of Moc09g14060 vs. NCBI nr
Match: XP_020415508.1 (uncharacterized protein LOC109948021 [Prunus persica])

HSP 1 Score: 101.3 bits (251), Expect = 7.0e-18
Identity = 50/112 (44.64%), Postives = 73/112 (65.18%), Query Frame = 0


Query: 27   YEATCLRIYSDSMLVVNQVKGDYQLDLLEELQEGTKQRMMTYKERSAQHYNVRMRPRAFQ 86
            Y+AT     +D  L +N       LD ++EL++ +  R + YK+R A++Y+ R++PRAF+
Sbjct: 933  YDATA----NDEQLALN-------LDFIDELRDQSSMRNVAYKQRIAKYYDSRVKPRAFK 992

Query: 87   VGDMVLRKVLPSTKNPTNGVLCLTWEGPYVVIKLAQPETYYLSDAMGKVLTH 139
            +GD V+RKV  +TKNP  G L  TWEGPY +IK+ +P TY L D+ GK L H
Sbjct: 993  IGDWVMRKVSLATKNPNEGTLGPTWEGPYEIIKICRPGTYQLRDSTGKTLPH 1033

[Close]

BLAST of Moc09g14060 vs. NCBI nr
Match: GFY92744.1 (hypothetical protein Acr_08g0011400 [Actinidia rufa])

HSP 1 Score: 101.3 bits (251), Expect = 7.0e-18
Identity = 60/173 (34.68%), Postives = 87/173 (50.29%), Query Frame = 0


Query: 1   MRFEFKTLNNETKYKDFVVGLKASQEYEATCLRIYSDSMLVVNQVKGD------------ 60
           +R  FK  NN+ +Y+  + GL+   E     L ++S+S LVVNQV+GD            
Sbjct: 224 IRIGFKATNNKAEYEALLTGLRVVAELGVDSLDVFSNSQLVVNQVQGDCLTKDTRMVAYL 283

Query: 61  -----------------------YQLDLLEELQEGTKQRMMTYKERSAQHYNVRMRPRAF 120
                                    LDLL+E +E  +   + YK    ++YN +++ ++F
Sbjct: 284 DEIGVPSFRTSNFNVENNEAKLRLNLDLLDEKRENAEISQVAYKHPITKYYNQKVKHKSF 343

Query: 121 QVGDMVLRKVLPSTKNPTNGVLCLTWEGPYVVIKLAQPETYYLSDAMGKVLTH 139
             G +VLRKV  STK P  G L  TWEGPY VIK+++P TY+L D  GK L +
Sbjct: 344 LSGYLVLRKVSLSTKEPNVGKLGPTWEGPYRVIKVSRPGTYWLKDTSGKALPY 396

[Close]

BLAST of Moc09g14060 vs. NCBI nr
Match: XP_020415514.1 (uncharacterized protein LOC109948028 [Prunus persica])

HSP 1 Score: 100.9 bits (250), Expect = 9.1e-18
Identity = 51/112 (45.54%), Postives = 72/112 (64.29%), Query Frame = 0


Query: 27  YEATCLRIYSDSMLVVNQVKGDYQLDLLEELQEGTKQRMMTYKERSAQHYNVRMRPRAFQ 86
           Y+AT     +D  L +N       LD +EEL++ +  R   YK+R A++Y+ R++PRAF+
Sbjct: 642 YDATA----NDEQLALN-------LDFIEELRDQSSMRNAAYKQRIAKYYDSRVKPRAFK 701

Query: 87  VGDMVLRKVLPSTKNPTNGVLCLTWEGPYVVIKLAQPETYYLSDAMGKVLTH 139
           +GD V+RKV  +TKNP  G L  TWEGPY +IK+ +P TY L D+ GK L H
Sbjct: 702 MGDWVMRKVSLATKNPNEGTLGPTWEGPYEIIKICRPGTYQLRDSTGKTLPH 742

[Close]

BLAST of Moc09g14060 vs. ExPASy TrEMBL
Match: A0A7J0F238 (RNase H domain-containing protein OS=Actinidia rufa OX=165716 GN=Acr_08g0011400 PE=4 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 3.4e-18
Identity = 60/173 (34.68%), Postives = 87/173 (50.29%), Query Frame = 0


Query: 1   MRFEFKTLNNETKYKDFVVGLKASQEYEATCLRIYSDSMLVVNQVKGD------------ 60
           +R  FK  NN+ +Y+  + GL+   E     L ++S+S LVVNQV+GD            
Sbjct: 224 IRIGFKATNNKAEYEALLTGLRVVAELGVDSLDVFSNSQLVVNQVQGDCLTKDTRMVAYL 283

Query: 61  -----------------------YQLDLLEELQEGTKQRMMTYKERSAQHYNVRMRPRAF 120
                                    LDLL+E +E  +   + YK    ++YN +++ ++F
Sbjct: 284 DEIGVPSFRTSNFNVENNEAKLRLNLDLLDEKRENAEISQVAYKHPITKYYNQKVKHKSF 343

Query: 121 QVGDMVLRKVLPSTKNPTNGVLCLTWEGPYVVIKLAQPETYYLSDAMGKVLTH 139
             G +VLRKV  STK P  G L  TWEGPY VIK+++P TY+L D  GK L +
Sbjct: 344 LSGYLVLRKVSLSTKEPNVGKLGPTWEGPYRVIKVSRPGTYWLKDTSGKALPY 396

[Close]

BLAST of Moc09g14060 vs. ExPASy TrEMBL
Match: A0A2N9J1F3 (Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS58033 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 7.5e-18
Identity = 50/109 (45.87%), Postives = 71/109 (65.14%), Query Frame = 0


Query: 30   TCLRIYSDSMLVVNQVKGDYQLDLLEELQEGTKQRMMTYKERSAQHYNVRMRPRAFQVGD 89
            T  R+   S  V N+ +    LDL++E++E  + R+  Y+ER A+HYN R++PR   VGD
Sbjct: 1514 TPFRMTFGSEAVENEEQLRLNLDLIDEVRETAETRVKRYQERMARHYNSRVKPRQLSVGD 1573

Query: 90   MVLRKVLPSTKNPTNGVLCLTWEGPYVVIKLAQPETYYLSDAMGKVLTH 139
            +VLRKV  +TKNP+ G L   WEGPY VI++ +P TY+L D  G+ L H
Sbjct: 1574 LVLRKVTLATKNPSEGKLGPNWEGPYKVIEIRRPGTYHLEDMNGRRLPH 1622

[Close]

BLAST of Moc09g14060 vs. ExPASy TrEMBL
Match: A0A2N9IPQ6 (Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS55399 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.7e-17
Identity = 46/88 (52.27%), Postives = 63/88 (71.59%), Query Frame = 0


Query: 51   LDLLEELQEGTKQRMMTYKERSAQHYNVRMRPRAFQVGDMVLRKVLPSTKNPTNGVLCLT 110
            LDL++E++E  K RM  Y+E+ A+HYN R++PR   VGD+VLRKV  +TKNP+ G L   
Sbjct: 1592 LDLIDEVRETAKTRMKRYQEKMARHYNSRVKPRQLSVGDLVLRKVTLATKNPSEGKLGPN 1651

Query: 111  WEGPYVVIKLAQPETYYLSDAMGKVLTH 139
            WEGPY VI++ +P TY+L D  G+ L H
Sbjct: 1652 WEGPYKVIEIRRPGTYHLEDMDGRRLPH 1679

[Close]

BLAST of Moc09g14060 vs. ExPASy TrEMBL
Match: A0A2N9IPQ6 (Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS55399 PE=4 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.5e-02
Identity = 29/80 (36.25%), Postives = 45/80 (56.25%), Query Frame = 0


Query: 1    MRFEFKTLNNETKYKDFVVGLKASQEYEATCLRIYSDSMLVVNQVKGDYQLDLLEELQEG 60
            +R ++ T NNE +Y+  +VGLK ++   AT L + SDS LVV QV GDY     E  +  
Sbjct: 1198 IRLQYPTTNNEAEYEALLVGLKTAKILGATELDVRSDSQLVVGQVNGDY-----EAKERR 1257

Query: 61   TKQRMMTYKERSAQHYNVRM 81
             +Q +   + + +Q   VR+
Sbjct: 1258 MQQYLQLVRHQISQFREVRL 1272

HSP 2 Score: 97.8 bits (242), Expect = 3.7e-17
Identity = 50/97 (51.55%), Postives = 66/97 (68.04%), Query Frame = 0


Query: 42  VNQVKGDYQLDLLEELQEGTKQRMMTYKERSAQHYNVRMRPRAFQVGDMVLRKVLPSTKN 101
           +N+ +    LDLLEE +E  + R M YK + A++YN R+R R+F  GD+VLRKV  STK 
Sbjct: 305 INEAELRVNLDLLEEKRESAELRQMAYKCQVAKYYNQRVRHRSFLPGDLVLRKVTLSTKE 364

Query: 102 PTNGVLCLTWEGPYVVIKLAQPETYYLSDAMGKVLTH 139
           P  G L  TWE PY VIK+++P TY+L D  GKVL+H
Sbjct: 365 PNTGKLGPTWERPYKVIKVSRPGTYWLEDLDGKVLSH 401

[Close]

BLAST of Moc09g14060 vs. ExPASy TrEMBL
Match: A0A2N9GIU6 (Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS27250 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 3.7e-17
Identity = 45/88 (51.14%), Postives = 63/88 (71.59%), Query Frame = 0


Query: 51   LDLLEELQEGTKQRMMTYKERSAQHYNVRMRPRAFQVGDMVLRKVLPSTKNPTNGVLCLT 110
            LDL++E++E  + RM  Y+E+ A+HYN R++PR   VGD+VLRKV  +TKNP+ G L   
Sbjct: 1463 LDLIDEVRETAETRMKRYQEKMARHYNSRVKPRQLSVGDLVLRKVTLATKNPSEGKLGPN 1522

Query: 111  WEGPYVVIKLAQPETYYLSDAMGKVLTH 139
            WEGPY VI++ +P TY+L D  G+ L H
Sbjct: 1523 WEGPYKVIEIRRPGTYHLEDMNGRRLPH 1550

The following BLAST results are available for this feature:

BLAST of Moc09g14060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_030945303.1	3.1e-18	38.57	uncharacterized protein LOC115969823 [Quercus lobata]	[more]
XP_020417276.1	5.3e-18	39.19	uncharacterized protein LOC109948519 [Prunus persica]	[more]
XP_020415508.1	7.0e-18	44.64	uncharacterized protein LOC109948021 [Prunus persica]	[more]
GFY92744.1	7.0e-18	34.68	hypothetical protein Acr_08g0011400 [Actinidia rufa]	[more]
XP_020415514.1	9.1e-18	45.54	uncharacterized protein LOC109948028 [Prunus persica]	[more]

BLAST of Moc09g14060 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc09g14060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A7J0F238	3.4e-18	34.68	RNase H domain-containing protein OS=Actinidia rufa OX=165716 GN=Acr_08g0011400 ...	[more]
A0A2N9J1F3	7.5e-18	45.87	Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS58033 PE=4 SV=1	[more]
A0A2N9IPQ6	1.7e-17	52.27	Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS55399 PE=4 SV=1	[more]
A0A2N9IPQ6	1.5e-02	36.25	Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS55399 PE=4 SV=1	[more]
A0A2N9GIU6	3.7e-17	51.14	Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS27250 PE=4 SV=1	[more]

Pages

BLAST of Moc09g14060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 1..88 e-value: 3.2E-7 score: 32.2
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 4..62

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc09g14060.1	Moc09g14060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding