Moc09g13520 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g13520
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Pectinesterase
Location	chr9: 11444198 .. 11444587 (-)
RNA-Seq Expression	Moc09g13520
Synteny	Moc09g13520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGTTCCAGGATTTCGACCAAATCTCCGAGCGGCGACGTGCTGAGAGGGCTCGCAAGTTCCGCCAGAGGGTCACCATCGCTGTGGTTTCAGTGTTCGTGGTCATCCTTCTGGTTGGGGCCGCCGTGTTCGTCCAGATCAACAACTCCCCCGCCAAATCCGGCCGTAATTCCGACGACGACAGCGGCGGCGCTGCCACCTCCAACTCCAAGCAACAACCCGCCGCTCCGGTCACCAAAGATGTGTCTCGTGTGGACAAGCTCATTGTCCTGATCTGTAACTCCACCGACTACAAAGGCAAATGCGAGAGCATTCTTAAGGACGCCAAACCCCGACTCCTCCGACCCCAAGGACCTGATCAAGCTCGCCATCTCCGCCACCGTGGATGA

mRNA sequence

ATGGTGTTCCAGGATTTCGACCAAATCTCCGAGCGGCGACGTGCTGAGAGGGCTCGCAAGTTCCGCCAGAGGGTCACCATCGCTGTGGTTTCAGTGTTCGTGGTCATCCTTCTGGTTGGGGCCGCCGTGTTCGTCCAGATCAACAACTCCCCCGCCAAATCCGGCCGTAATTCCGACGACGACAGCGGCGGCGCTGCCACCTCCAACTCCAAGCAACAACCCGCCGCTCCGGTCACCAAAGATGTGTCTCGTGTGGACAAGCTCATTGTCCTGATCTGTAACTCCACCGACTACAAAGGCAAATGCGAGAGCATTCTTAAGGACGCCAAACCCCGACTCCTCCGACCCCAAGGACCTGATCAAGCTCGCCATCTCCGCCACCGTGGATGA

Coding sequence (CDS)

ATGGTGTTCCAGGATTTCGACCAAATCTCCGAGCGGCGACGTGCTGAGAGGGCTCGCAAGTTCCGCCAGAGGGTCACCATCGCTGTGGTTTCAGTGTTCGTGGTCATCCTTCTGGTTGGGGCCGCCGTGTTCGTCCAGATCAACAACTCCCCCGCCAAATCCGGCCGTAATTCCGACGACGACAGCGGCGGCGCTGCCACCTCCAACTCCAAGCAACAACCCGCCGCTCCGGTCACCAAAGATGTGTCTCGTGTGGACAAGCTCATTGTCCTGATCTGTAACTCCACCGACTACAAAGGCAAATGCGAGAGCATTCTTAAGGACGCCAAACCCCGACTCCTCCGACCCCAAGGACCTGATCAAGCTCGCCATCTCCGCCACCGTGGATGA

Protein sequence

MVFQDFDQISERRRAERARKFRQRVTIAVVSVFVVILLVGAAVFVQINNSPAKSGRNSDDDSGGAATSNSKQQPAAPVTKDVSRVDKLIVLICNSTDYKGKCESILKDAKPRLLRPQGPDQARHLRHRG

Homology

[Close]

BLAST of Moc09g13520 vs. NCBI nr
Match: XP_008460558.1 (PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Cucumis melo] >KAA0045465.1 putative pectinesterase/pectinesterase inhibitor 45 [Cucumis melo var. makuwa] >TYK00244.1 putative pectinesterase/pectinesterase inhibitor 45 [Cucumis melo var. makuwa])

HSP 1 Score: 133.7 bits (335), Expect = 1.2e-27
Identity = 73/108 (67.59%), Postives = 88/108 (81.48%), Query Frame = 0


Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVVSVFVVILLVGAAVFVQINNSPAKSGRNSDD 60
           MVFQDFDQISERRRAERARKFR+RVTIAVVS  V++L+V AAVFVQ+++S   + +N D 
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLVVAAAVFVQVSSSSKSTTKNGD- 60

Query: 61  DSGGAATSNSKQQPAAPVTKDVSRVDKLIVLICNSTDYKGKCESILKD 109
              G++T NSK +P  P TK VSRV+K+I +ICNSTDYKGKCES LKD
Sbjct: 61  --SGSSTGNSK-KPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKD 104

[Close]

BLAST of Moc09g13520 vs. NCBI nr
Match: XP_004142361.1 (putative pectinesterase/pectinesterase inhibitor 45 [Cucumis sativus] >KGN52371.1 hypothetical protein Csa_008584 [Cucumis sativus])

HSP 1 Score: 129.8 bits (325), Expect = 1.7e-26
Identity = 74/108 (68.52%), Postives = 88/108 (81.48%), Query Frame = 0


Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVVSVFVVILLVGAAVFVQINNSPAKSGRNSDD 60
           MVFQDFDQISERRRAERARKFR+RV IAVVS  V++L+V AAVFVQ+++S  KS   +DD
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVIIAVVSASVILLVVAAAVFVQVSSS--KSTTKNDD 60

Query: 61  DSGGAATSNSKQQPAAPVTKDVSRVDKLIVLICNSTDYKGKCESILKD 109
              G+++ NSK QP  P TK VSRV+K+I +ICNSTDYKGKCES LKD
Sbjct: 61  --SGSSSGNSK-QPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKD 103

[Close]

BLAST of Moc09g13520 vs. NCBI nr
Match: XP_038893171.1 (putative pectinesterase/pectinesterase inhibitor 45 [Benincasa hispida])

HSP 1 Score: 123.6 bits (309), Expect = 1.2e-24
Identity = 69/108 (63.89%), Postives = 86/108 (79.63%), Query Frame = 0


Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVVSVFVVILLVGAAVFVQINNSPAKSGRNSDD 60
           MVFQDFDQISERRRAERARKFR+RVTIAVVSV V++L++ AAVFVQ+++S   + +N + 
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSVSVILLVIAAAVFVQVSSSSKSTTKNGNS 60

Query: 61  DSGGAATSNSKQQPAAPVTKDVSRVDKLIVLICNSTDYKGKCESILKD 109
            S  ++  NSK+     V K VSRV+K+I +ICNSTDYKGKCES LKD
Sbjct: 61  KS-SSSNENSKETVPDDV-KQVSRVEKMITMICNSTDYKGKCESTLKD 106

[Close]

BLAST of Moc09g13520 vs. NCBI nr
Match: XP_023530461.1 (putative pectinesterase/pectinesterase inhibitor 45 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 117.9 bits (294), Expect = 6.7e-23
Identity = 69/125 (55.20%), Postives = 86/125 (68.80%), Query Frame = 0


Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVVSVFVVILLVGAAVFVQINNSPAKSGRNSDD 60
           MVFQDFDQISERRR ERARKFRQRV I VV   V+ILL+GAAVF+Q++ +P+ S + S+ 
Sbjct: 1   MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLIGAAVFIQVSPAPSSSSKTSNK 60

Query: 61  DSGGAATSNSKQQPAAPV-----TKDVSRVDKLIVLICNSTDYKGKCESILKD---AKPR 118
           D        SK+ P +       +KD S  +K+IV+ICNSTDYK KCESILKD   A P+
Sbjct: 61  D------GKSKKHPKSNADTDTESKDASSAEKMIVMICNSTDYKEKCESILKDGLKANPK 119

[Close]

BLAST of Moc09g13520 vs. NCBI nr
Match: KAG6596157.1 (putative pectinesterase/pectinesterase inhibitor 45, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 117.9 bits (294), Expect = 6.7e-23
Identity = 66/108 (61.11%), Postives = 78/108 (72.22%), Query Frame = 0


Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVVSVFVVILLVGAAVFVQINNSPAKSGRNSDD 60
           MVFQDFDQISERRRAERARKFR RV IAVVS F++++LV AAVFVQ+ + P KS      
Sbjct: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQV-SPPTKSTTRDGA 60

Query: 61  DSGGAATSNSKQQPAAPVTKDVSRVDKLIVLICNSTDYKGKCESILKD 109
            SG + +  S +  A    K VSR  K+I +ICNSTDYKGKCES LK+
Sbjct: 61  GSGSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKGKCESTLKE 107

[Close]

BLAST of Moc09g13520 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 3.8e-13
Identity = 46/114 (40.35%), Postives = 66/114 (57.89%), Query Frame = 0


Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVVSVFVV--ILLVGAAVFVQINNSPAKSGRNS 60
           M FQDFD+I ER  AER RKFR+R+ + VVSV VV   ++ GA  +V   N   + G+ +
Sbjct: 1   MAFQDFDKIQERVNAERKRKFRKRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTT 60

Query: 61  DDDSGGAATSNSKQQPAAP----VTKDVSRVDKLIVLICNSTDYKGKCESILKD 109
           ++ S  + T +    P  P     T    +VDK+I  +CNST YK  C++ LK+
Sbjct: 61  NNKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKN 114

[Close]

BLAST of Moc09g13520 vs. ExPASy Swiss-Prot
Match: Q7Y201 (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3702 GN=PME13 PE=2 SV=2)

HSP 1 Score: 58.2 bits (139), Expect = 8.2e-08
Identity = 42/128 (32.81%), Postives = 61/128 (47.66%), Query Frame = 0


Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVVSVFVVILLVGAAVFVQI------------- 60
           M FQDFD+I ER  A R RKFR+R+ +  VS+ VV+  +    F  +             
Sbjct: 1   MAFQDFDKIQERVNANRKRKFRKRIIVGTVSLLVVVAAIVGGAFAYVAYEKRNEQQQQQQ 60

Query: 61  ---NNSPAKSGRN----SDDDSGGAATSNSKQQPAAPVTKDVSRVDKLIVLICNSTDYKG 109
              N++ + SG N    SD  S    T + K   +A  +    + DK+I  +C+ST Y  
Sbjct: 61  QAKNHNKSGSGNNVVKDSDKKSPSPPTPSQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQ 120

[Close]

BLAST of Moc09g13520 vs. ExPASy TrEMBL
Match: A0A5D3BLU5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2460G00020 PE=3 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 5.7e-28
Identity = 73/108 (67.59%), Postives = 88/108 (81.48%), Query Frame = 0


Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVVSVFVVILLVGAAVFVQINNSPAKSGRNSDD 60
           MVFQDFDQISERRRAERARKFR+RVTIAVVS  V++L+V AAVFVQ+++S   + +N D 
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLVVAAAVFVQVSSSSKSTTKNGD- 60

Query: 61  DSGGAATSNSKQQPAAPVTKDVSRVDKLIVLICNSTDYKGKCESILKD 109
              G++T NSK +P  P TK VSRV+K+I +ICNSTDYKGKCES LKD
Sbjct: 61  --SGSSTGNSK-KPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKD 104

[Close]

BLAST of Moc09g13520 vs. ExPASy TrEMBL
Match: A0A1S3CCA7 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499351 PE=3 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 5.7e-28
Identity = 73/108 (67.59%), Postives = 88/108 (81.48%), Query Frame = 0


Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVVSVFVVILLVGAAVFVQINNSPAKSGRNSDD 60
           MVFQDFDQISERRRAERARKFR+RVTIAVVS  V++L+V AAVFVQ+++S   + +N D 
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLVVAAAVFVQVSSSSKSTTKNGD- 60

Query: 61  DSGGAATSNSKQQPAAPVTKDVSRVDKLIVLICNSTDYKGKCESILKD 109
              G++T NSK +P  P TK VSRV+K+I +ICNSTDYKGKCES LKD
Sbjct: 61  --SGSSTGNSK-KPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKD 104

[Close]

BLAST of Moc09g13520 vs. ExPASy TrEMBL
Match: A0A0A0KX33 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G630740 PE=3 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 8.2e-27
Identity = 74/108 (68.52%), Postives = 88/108 (81.48%), Query Frame = 0


Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVVSVFVVILLVGAAVFVQINNSPAKSGRNSDD 60
           MVFQDFDQISERRRAERARKFR+RV IAVVS  V++L+V AAVFVQ+++S  KS   +DD
Sbjct: 1   MVFQDFDQISERRRAERARKFRKRVIIAVVSASVILLVVAAAVFVQVSSS--KSTTKNDD 60

Query: 61  DSGGAATSNSKQQPAAPVTKDVSRVDKLIVLICNSTDYKGKCESILKD 109
              G+++ NSK QP  P TK VSRV+K+I +ICNSTDYKGKCES LKD
Sbjct: 61  --SGSSSGNSK-QPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKD 103

[Close]

BLAST of Moc09g13520 vs. ExPASy TrEMBL
Match: A0A6J1HGL4 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111463347 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.6e-22
Identity = 65/108 (60.19%), Postives = 77/108 (71.30%), Query Frame = 0


Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVVSVFVVILLVGAAVFVQINNSPAKSGRNSDD 60
           MVFQDFDQISERRRAERARKFR RV IAVVS F++++LV AAVFVQ+ + P KS      
Sbjct: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVLVVAAVFVQV-SPPTKSTTRDGA 60

Query: 61  DSGGAATSNSKQQPAAPVTKDVSRVDKLIVLICNSTDYKGKCESILKD 109
            SG + +  S +  A    K VSR  K+I +ICNSTDYK KCES LK+
Sbjct: 61  GSGSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKAKCESTLKE 107

[Close]

BLAST of Moc09g13520 vs. ExPASy TrEMBL
Match: A0A6J1I3R6 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470280 PE=3 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 7.9e-22
Identity = 66/110 (60.00%), Postives = 79/110 (71.82%), Query Frame = 0


Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVVSVFVVILLVGAAVFVQINNSPAKSGRNSDD 60
           MVFQDFDQISERRRAERARKFR RV IAVVS F++++ V AAVFVQ+ + P KS      
Sbjct: 1   MVFQDFDQISERRRAERARKFRNRVIIAVVSTFLILVFVVAAVFVQV-SPPTKSTTRDGA 60

Query: 61  DSGGAATSNSK---QQPAAPVTKDVSRVDKLIVLICNSTDYKGKCESILK 108
            +G +++S SK   +  A    K VSR  K+I +ICNSTDYKGKCES LK
Sbjct: 61  GAGSSSSSRSKGSSKDHATSDVKQVSRAQKMITMICNSTDYKGKCESTLK 109

[Close]

BLAST of Moc09g13520 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 75.9 bits (185), Expect = 2.7e-14
Identity = 46/114 (40.35%), Postives = 66/114 (57.89%), Query Frame = 0


Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVVSVFVV--ILLVGAAVFVQINNSPAKSGRNS 60
           M FQDFD+I ER  AER RKFR+R+ + VVSV VV   ++ GA  +V   N   + G+ +
Sbjct: 1   MAFQDFDKIQERVNAERKRKFRKRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTT 60

Query: 61  DDDSGGAATSNSKQQPAAP----VTKDVSRVDKLIVLICNSTDYKGKCESILKD 109
           ++ S  + T +    P  P     T    +VDK+I  +CNST YK  C++ LK+
Sbjct: 61  NNKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKN 114

[Close]

BLAST of Moc09g13520 vs. TAIR 10
Match: AT2G26450.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 58.2 bits (139), Expect = 5.8e-09
Identity = 42/128 (32.81%), Postives = 61/128 (47.66%), Query Frame = 0


Query: 1   MVFQDFDQISERRRAERARKFRQRVTIAVVSVFVVILLVGAAVFVQI------------- 60
           M FQDFD+I ER  A R RKFR+R+ +  VS+ VV+  +    F  +             
Sbjct: 1   MAFQDFDKIQERVNANRKRKFRKRIIVGTVSLLVVVAAIVGGAFAYVAYEKRNEQQQQQQ 60

Query: 61  ---NNSPAKSGRN----SDDDSGGAATSNSKQQPAAPVTKDVSRVDKLIVLICNSTDYKG 109
              N++ + SG N    SD  S    T + K   +A  +    + DK+I  +C+ST Y  
Sbjct: 61  QAKNHNKSGSGNNVVKDSDKKSPSPPTPSQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQ 120

The following BLAST results are available for this feature:

BLAST of Moc09g13520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008460558.1	1.2e-27	67.59	PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Cucumis melo] >K...	[more]
XP_004142361.1	1.7e-26	68.52	putative pectinesterase/pectinesterase inhibitor 45 [Cucumis sativus] >KGN52371....	[more]
XP_038893171.1	1.2e-24	63.89	putative pectinesterase/pectinesterase inhibitor 45 [Benincasa hispida]	[more]
XP_023530461.1	6.7e-23	55.20	putative pectinesterase/pectinesterase inhibitor 45 [Cucurbita pepo subsp. pepo]	[more]
KAG6596157.1	6.7e-23	61.11	putative pectinesterase/pectinesterase inhibitor 45, partial [Cucurbita argyrosp...	[more]

BLAST of Moc09g13520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SMY6	3.8e-13	40.35	Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3...	[more]
Q7Y201	8.2e-08	32.81	Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Moc09g13520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BLU5	5.7e-28	67.59	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2460G0002...	[more]
A0A1S3CCA7	5.7e-28	67.59	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499351 PE=3 SV=1	[more]
A0A0A0KX33	8.2e-27	68.52	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G630740 PE=3 SV=1	[more]
A0A6J1HGL4	1.6e-22	60.19	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111463347 PE=3 SV=1	[more]
A0A6J1I3R6	7.9e-22	60.00	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470280 PE=3 SV=1	[more]

BLAST of Moc09g13520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G33230.1	2.7e-14	40.35	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G26450.1	5.8e-09	32.81	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 50..77
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 50..80

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc09g13520.1	Moc09g13520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity