Moc09g12010 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g12010
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	ribonuclease MC-like
Location	chr9: 10236590 .. 10241678 (+)
RNA-Seq Expression	Moc09g12010
Synteny	Moc09g12010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGACCTCGTCCACGAGAATGATGAGACAGTGGGCGATAATTGGCCATCATGGCAGTGGATTGGAATATGGACTCTACACCTTTGGCTTGAGAAAAGATTGCAGCTTCTTTTGTTTTAAGAAGAACATGAAGTTCTTCCACACGAACAGGACTGGACCATACCCAAAACTAGGTACGAAAATCATTGTATTCATGAGGCAAGCCATTCAAAGTGTAGATTAGGATGTCCTCCTCGTCGATATGGACAGAGATAGTAGATAACTTGTCCTGAATAGTTTTGATTCGCTGAACATAATCATCGATGGACTCTATCCCCTTTTTCTGAATTTTCTGCAATTCCGATTTCAAATCAATTATATTAGTATGAGTAAAAGATGAGTAACGATGTTCAAGAGATTCCCAAACCTGTTTCTAGCTAGTGCAACCAACAACAAGAGATAGGGCGGACTGGGACAACATAGAATTGATTAGAGTTCGCAATACATGATCCTAGGAGTGCCACAAGGCATATTTGGGATGGAGCTTTGTTGTCTCGCCTTGTTGAATATGTTGATCGGGGCAAATACATGAGCCGTCAATGTAACCAAAGAGATTGTGTGCCTTCAAGATGGAGTCGAGTTAAAGATAATAGCAAAGAGTAGTTGTTGGAGTCAAGTCAGACAGAAACAAGAGTACAAATATTTGAGAGTAGGAATAGCGGTGAAGGAGAGAATTGACGATCGAGGGTGAAAGATGTGGGAAATTGGTTGATTTCTGTGTGGAATTTGAAAGAAGTATTTGTATCGGTCATTGTGTAATTCGCTACGTGGGAAAGAGAATTGGGATAATTAAGAATTGAATGAGAAAGAGAAAAATAAAAAATAAAAAATAAAATAGAAGGTGGGTTATGCCAAAATGTATGTGGGAGAGGGGAAGAAAAAAAAATTCATGTGGGAATTGAGTAAAAGATGATGTGGGAATGAGGCCAAAAATGGAAAAGGGGTGAAATGTGGGTGGGGTAATTAAAACTGAAATTTAGAAGGGAGAAAAAAATAATAAAATTTGGCAAGTTTGGAATAGGGTCGACGCGGCGACTGTGTTACCAAGATCGAAGGAAGAAGACGTATGTCGAAAGCTCAGATACTATGAGCCCCTGATTTATTCATTCGTCTTAGGAGGAATCAACAAAGGACTGATGATGTGGGTTCGAACACAGGACCTCTTGTTCTGATATCATGTTAAAGATAAATAAACCACCACTCAACCCAAAAACTTAAGCAAGATAGATTGGATAAATTTAATTCATTTATATATATTCCCAACAAAATCATATAAAAATAAAGTTGAGCGAATAGCTCTTGAGAAGATCTTGCACACATATCATCAATATATCTTTTGTTATATATATTTGATCAGTTGAACCTAATTCAAGTCTCCGTGATTAGTCAACAATGTTAAGGATCTCACATTGATAAAAGAAATTACATTACATAGGTTTATAATAGACATAGACTATTCCTCTTATTGCCAATTGGTTTTGAGATAGAACCCTATAAATATTGATAAACAACTATATTTAAATGTACTAAAAAAAACTATATTTAAATACTATTTTAGTTCCTATATTCTTAATTTTGTTTATTTTAATCCTAGTTGTACTTTCAAAATTTTTATTTTGGTCCTGGTACTTTTGAGTTTTGTCAATTTTAGTCCCTAGACTTTCAAAATATCTGTTTTCATATATATTTTTTGAAAGCTACTTTTTTAAACATTCATTTACATTTTTATCTGTTTTAATTTTTGTTACAATTCCGATATGAAACTAAACTCAATAAAATTAGAATTCATGTTAAACTTTTACAAACAAAGACCAAAATTGATACTTTTAAAAGTATAACAAATGTTTTGAAAGCGAATAATGATTAAAATGAACAAAAAATCAAAATACATGGACAAAAAATGAATGTTTTAAAAGAATAATAACCAAAATGTACTAAAGCTAAAAACACATCGACCAAATAATATTTAAACCCAACAAATATTATAATTAGGTACATTTTATATTTGATGAAGTTAAATATTTATAATAATTAGAGAATAATTTAAAAAATTCATAGTCATAACAATGATTTGAAATATTCGTAGTATGAATATGATATAAATATTAATAATTTATATATGATTTTATTTTTTAAATCCGAGCATAGCTCAACTGATTAAGGCAACTATTTCCTACTAAAAGATTGTGAGTTTGAATGCCTCGCTCCACACGTTGTCATAGTAATTTATTTTTTTAAGAAAATTATCAATTGGTTTCCATAAGGTCTTTTTGTTAGTTTTTTTTTTTTTTGAAATATTTATCAGGGTCGATATTTCTATGGATGTATAAGGTTCAACATTGTTTGGTTGATGATAGAACCTAGTTTTGTGAACACTAAATGTTCAAGGACGATTTCAACCCAAATAATCTTTAAATACAAGTTTTTCTTCCAAATGACTAAAATAATTTTTACGGAAAACCTTGACTAAAATATAAAAAATGAAAGTTGAAAGATTCTATCATTATTAAGTCGACTTAAACTTAGTTCAACGGGTTAATATATCAATTTCTAATCAAAAGATTATAAGTTGGAATTTCATCTGTATTTTATTTTATTGTGTTTACAAAATAAAAATAAAATTAAAAAGAATAAAACAAAAGAACTAGAAATAATAATTAAAGGAAATTCATAAATCTCTCCTGTGAAGACAAGCCATAGACGCCACCCTTCCTCCTCATACGTTTCCGACAGTTACCCAAAATTGAATAGTGTAATATTGAAGCTTTGGATAATTCTTTATTCTTAATTATTCAAAGCCCATAACCACAATAATTGCAAGAGAAACAAACAAATCTTTAAATTTTTAGAATAAATAGGGGCTGAGAGGAACACCATTCTCACCAAGCTTTTAAATTCGGTAGGTTCGAATTTGAAACCACGAGAGGAGACATGAAAACGTCGAGGAGAGAAATCGTGTTCATGTTTGTCTTAGCAATAACCATCTTTCTCCCCATTGTAAAATCACAAACTTTTGATGACTTCTGGTTCGTTCAACAATGGCCACCCGCTGTTTGTAGTTTCCAACAAGGACGATGTGTCGGACAAGGGCTACGAACCTTCACAATCCATGGCGTGTGGCCGCAAAAGGGAGGCACCTCTGTTGTTAATTGCCCTGGCACTCCCTTCGATTTCACGAAGGTAAAACTCAAACACCAAAATTCTCAAATTTCACTAATTTGCCGTAATTTAAAAAAGCATATATCATACTTAGAGGGTCTATCTAAGAATGATTTGGATATCGCTAAAATCACTATTATTTGAAGATAACATAATTCACAATAATTCTTAAGGGATTTTAAAAAAATACAAAATCACTTTTAAGGGTATAGGTTGGGATGCTAAGGTGATTCCCGTCGCTTTAAAATTTGATTTTTTTTTCTCATTTCTAAAATTCTTGCACTGTCTAACTTTATTCTATCTTTAATTTTTGTTATTCTCTATAATTTTTTTCTCCAAAATTTTGGTAATATCTTTCTAAATTTTTGGTTTTCATCTCTTTTCTAAATTTATCTTCGTGTCTAAAATTTTTGTCTCTTCCCCTCTAAAATTTCCCTCTCTAAGATTTTTGTTCCCCTCTCACTTTTCTCTCTCTAAAAACTTTAGTCAAAATTCTCTCTAACATTCTTTTAATTATATAAAAATCTCTTGAAAAAGTGTATAATGAAAGCTTAGGATAACTATATAAAAATCTTTTTTTTTCCGCTAAAATCACTCATTTTAAAATCATTTTCAAACAAACCTTAAAGATTTCCTAGTTTTTCTCTAATTTGCATCTATTGCTCTCTTTGTTTCCCGTAAATTTTGAGAGATTTTAAATCTACATGAGTTTTAAATTAATTATGTTGTCTTTTTTTATGGCCAGAATTAGAGTGGTTTGAAATATGAATCTTGTCTTATTTTGAAAAAAAATTACAAACATAAAGGGTAGGAATTCACACGTTTTCATATAATTTTAACTCAAATTAACAGGATCATATTTGCAGCATAATTCAACGGTAATTAACATATATATATGTAATAAAGATGTTTTAAGTTCAATTCCCTCTATATGTTGTACTAAAAAAATATATGGTCATACTTTTAATATTTTTTTAATAAATCGAGATGATGTGATTTAAACCTATAGTTAGAATCCACATAACATGGAATTCACGTGATCTTTGATATGGCAAAAATCACTAATTTTACATTAGTTTGAAAAAAATATCTTAAAATGAAATTCTGTTATTTAAACGACACTTTTTAAACTTTATTTTCAAAAGTATCTTATTTTTATAATTTTTATAATGACACTATTTTTAACTTTGCCCGATCTAAACTTTTTATTTTATTTTATTTTTCAGCGACAACTTCTTAAAATCTTTCTAAATCAAAACAAAATTTAAAATTTAAAGTCATTTCAAGTACTTGAGAGATTTAAATTTAAAATTTAAAGTTTAAAGTCATATATCTATGTGTAAAATTTAATTTTTTTTATGTATGTATGTGTATATATTATATTTATAAAATTAAAATGTATAATTTTGAGTTTCCACTAAATTGATATACGACTTTAGCACCAAATAATATTAGATTTTAAGGATAGAATATTGTTTTTGTTTCAGATTTCAAGTTTAGAAACTGATTTGAATAGAGTATGGCCGAACGTTATAACGGGAGACAACAAATTTTTCTGGTCACATGAGTGGAACAAGCATGGGGTTTGCTCAGAGAGTAAATTCAACATGAAAGCGTACTTCCAAATGGCTATTAATATGAGAAATAGTATCGATCTTATGAATGCCCTCAAAGTTGGTGGGTTAGCACCAAACGGGCTCAGCAAGTCAAAGCAAAGAGTACAGAGTGCACTACGAACACACTTTGGGAAGGAACCTGTTCTTCGCTGTAGAGGAACTGGCAGCAACATCCGATTGATAGAGATTGTGATGTGTTTTGACGATGATGGTGTCACTCTTATCAACTGTAATCCTGTCAGTTCCAACTGTGCTAATAGCTTTATATTTTGA

mRNA sequence

ATGGCGACCTCGTCCACGAGAATGATGAGACAGTGGGCGATAATTGGCCATCATGGCAGTGGATTGGAATATGGACTCTACACCTTTGGCTTGAGAAAAGATTGCAGCTTCTTTTGTTTTAAGAAGAACATGAAGTTCTTCCACACGAACAGGACTGGACCATACCCAAAACTAGTTTCCAACAAGGACGATGTGTCGGACAAGGGCTACGAACCTTCACAATCCATGGCGTGTGGCCGCAAAAGGGAGGCACCTCTGTTGTTAATTGCCCTGGCACTCCCTTCGATTTCACGAAGAGTATGGCCGAACGTTATAACGGGAGACAACAAATTTTTCTGGTCACATGAGTGGAACAAGCATGGGGTTTGCTCAGAGAGTAAATTCAACATGAAAGCGTACTTCCAAATGGCTATTAATATGAGAAATAGTATCGATCTTATGAATGCCCTCAAAGTTGGTGGGTTAGCACCAAACGGGCTCAGCAAGTCAAAGCAAAGAGTACAGAGTGCACTACGAACACACTTTGGGAAGGAACCTGTTCTTCGCTGTAGAGGAACTGGCAGCAACATCCGATTGATAGAGATTGTGATGTGTTTTGACGATGATGGTGTCACTCTTATCAACTGTAATCCTGTCAGTTCCAACTGTGCTAATAGCTTTATATTTTGA

Coding sequence (CDS)

ATGGCGACCTCGTCCACGAGAATGATGAGACAGTGGGCGATAATTGGCCATCATGGCAGTGGATTGGAATATGGACTCTACACCTTTGGCTTGAGAAAAGATTGCAGCTTCTTTTGTTTTAAGAAGAACATGAAGTTCTTCCACACGAACAGGACTGGACCATACCCAAAACTAGTTTCCAACAAGGACGATGTGTCGGACAAGGGCTACGAACCTTCACAATCCATGGCGTGTGGCCGCAAAAGGGAGGCACCTCTGTTGTTAATTGCCCTGGCACTCCCTTCGATTTCACGAAGAGTATGGCCGAACGTTATAACGGGAGACAACAAATTTTTCTGGTCACATGAGTGGAACAAGCATGGGGTTTGCTCAGAGAGTAAATTCAACATGAAAGCGTACTTCCAAATGGCTATTAATATGAGAAATAGTATCGATCTTATGAATGCCCTCAAAGTTGGTGGGTTAGCACCAAACGGGCTCAGCAAGTCAAAGCAAAGAGTACAGAGTGCACTACGAACACACTTTGGGAAGGAACCTGTTCTTCGCTGTAGAGGAACTGGCAGCAACATCCGATTGATAGAGATTGTGATGTGTTTTGACGATGATGGTGTCACTCTTATCAACTGTAATCCTGTCAGTTCCAACTGTGCTAATAGCTTTATATTTTGA

Protein sequence

MATSSTRMMRQWAIIGHHGSGLEYGLYTFGLRKDCSFFCFKKNMKFFHTNRTGPYPKLVSNKDDVSDKGYEPSQSMACGRKREAPLLLIALALPSISRRVWPNVITGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGLAPNGLSKSKQRVQSALRTHFGKEPVLRCRGTGSNIRLIEIVMCFDDDGVTLINCNPVSSNCANSFIF

Homology

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BLAST of Moc09g12010 vs. NCBI nr
Match: XP_022155091.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 263.5 bits (672), Expect = 1.7e-66
Identity = 124/124 (100.00%), Postives = 124/124 (100.00%), Query Frame = 0


Query: 99  RVWPNVITGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGLAPN 158
           RVWPNVITGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGLAPN
Sbjct: 93  RVWPNVITGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGLAPN 152

Query: 159 GLSKSKQRVQSALRTHFGKEPVLRCRGTGSNIRLIEIVMCFDDDGVTLINCNPVSSNCAN 218
           GLSKSKQRVQSALRTHFGKEPVLRCRGTGSNIRLIEIVMCFDDDGVTLINCNPVSSNCAN
Sbjct: 153 GLSKSKQRVQSALRTHFGKEPVLRCRGTGSNIRLIEIVMCFDDDGVTLINCNPVSSNCAN 212

Query: 219 SFIF 223
           SFIF
Sbjct: 213 SFIF 216

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BLAST of Moc09g12010 vs. NCBI nr
Match: XP_022142280.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 238.4 bits (607), Expect = 5.8e-59
Identity = 107/124 (86.29%), Postives = 118/124 (95.16%), Query Frame = 0


Query: 99  RVWPNVITGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGLAPN 158
           RVWPNVITG+N+FFW HEWNKHG+CSESKF+MKAYFQMAINMR+S+DLMNAL+VGGLAPN
Sbjct: 97  RVWPNVITGNNRFFWGHEWNKHGICSESKFDMKAYFQMAINMRDSLDLMNALRVGGLAPN 156

Query: 159 GLSKSKQRVQSALRTHFGKEPVLRCRGTGSNIRLIEIVMCFDDDGVTLINCNPVSSNCAN 218
           G +KS+QRVQSA+R  FGKEPVLRCRGTG N+RLIEIVMCFDDDGVTLINCNP SSNCAN
Sbjct: 157 GRTKSRQRVQSAIRAQFGKEPVLRCRGTGRNVRLIEIVMCFDDDGVTLINCNPASSNCAN 216

Query: 219 SFIF 223
           SFIF
Sbjct: 217 SFIF 220

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BLAST of Moc09g12010 vs. NCBI nr
Match: BAA10891.1 (ribonuclease (RNase LC1) [Luffa aegyptiaca])

HSP 1 Score: 214.2 bits (544), Expect = 1.2e-51
Identity = 95/122 (77.87%), Postives = 109/122 (89.34%), Query Frame = 0


Query: 101 WPNVITGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGLAPNGL 160
           WPNVITG+NK+FW HEWNKHG+CS SKF+ +AYFQMAINMRNSIDL++AL+VGG+ PNG 
Sbjct: 93  WPNVITGNNKWFWGHEWNKHGICSVSKFDQQAYFQMAINMRNSIDLLSALRVGGVVPNGR 152

Query: 161 SKSKQRVQSALRTHFGKEPVLRCRGTGSNIRLIEIVMCFDDDGVTLINCNPVSSNCANSF 220
           SK++QRVQSA+R   GKEPVLRCRGTG   RL+EIVMCFDDDGVTLINCNP +SNC NSF
Sbjct: 153 SKARQRVQSAIRAQLGKEPVLRCRGTGRQSRLLEIVMCFDDDGVTLINCNPANSNCPNSF 212

Query: 221 IF 223
           IF
Sbjct: 213 IF 214

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BLAST of Moc09g12010 vs. NCBI nr
Match: XP_038905534.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 193.4 bits (490), Expect = 2.1e-45
Identity = 86/125 (68.80%), Postives = 105/125 (84.00%), Query Frame = 0


Query: 100 VWPNVITGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGLAPNG 159
           VWPNVITG+NKFFW HEWNKHG+CSESKF+  AYFQ AINMR+SIDL++ ++ GG+ PNG
Sbjct: 93  VWPNVITGNNKFFWGHEWNKHGICSESKFDQAAYFQTAINMRHSIDLLSVMRTGGVIPNG 152

Query: 160 LSKSKQRVQSALRTHFGKEPVLRCRGTGSN--IRLIEIVMCFDDDGVTLINCNPVSSNCA 219
            SK+KQRV+S + +HFGK+P+LRC+ T SN  + L E+VMCFDDDGVTL+NCN   SNCA
Sbjct: 153 ASKAKQRVESVISSHFGKDPILRCK-TASNGQVLLTEVVMCFDDDGVTLLNCNKARSNCA 212

Query: 220 NSFIF 223
            SFIF
Sbjct: 213 TSFIF 216

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BLAST of Moc09g12010 vs. NCBI nr
Match: XP_004147499.1 (ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [Cucumis sativus])

HSP 1 Score: 191.4 bits (485), Expect = 8.2e-45
Identity = 83/124 (66.94%), Postives = 102/124 (82.26%), Query Frame = 0


Query: 100 VWPNVITGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGLAPNG 159
           VWPNV+TG+NKFFW HEWNKHG+CSESKF+   YFQ AINMR+ IDL++ L+ GG+ PNG
Sbjct: 94  VWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKYFQTAINMRHGIDLLSVLRTGGVGPNG 153

Query: 160 LSKSKQRVQSALRTHFGKEPVLRC-RGTGSNIRLIEIVMCFDDDGVTLINCNPVSSNCAN 219
            SK+KQRV++A+ +HFGK+P+LRC + +   + L EIVMCFDDDGVTLINCN   SNCA 
Sbjct: 154 ASKAKQRVETAISSHFGKDPILRCKKASNGQVLLTEIVMCFDDDGVTLINCNKARSNCAG 213

Query: 220 SFIF 223
           SFIF
Sbjct: 214 SFIF 217

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BLAST of Moc09g12010 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.1e-28
Identity = 61/132 (46.21%), Postives = 83/132 (62.88%), Query Frame = 0


Query: 93  LPSISRRVWPNVITGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKV 152
           L S    +WPNV+  +N+ FWSHEW KHG CSES FN  AYF++A++MRN+ D++ AL+ 
Sbjct: 62  LQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALRP 121

Query: 153 GGLAPNGLSKSKQRVQSALRTHFGKEPVLRCRGTGSN--IRLIEIVMCFDDDGVTLINCN 212
               PNG +KS+Q ++  L+  FGK P LRCR         L+++V CF  DG TLI+C 
Sbjct: 122 HAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLIDC- 181

Query: 213 PVSSNCANSFIF 223
                C  +FIF
Sbjct: 182 -TRDTCGANFIF 191

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BLAST of Moc09g12010 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.6e-17
Identity = 41/115 (35.65%), Postives = 65/115 (56.52%), Query Frame = 0


Query: 98  RRVWPNVI--TGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGL 157
           ++ WP +   +G  + FW HEW KHG CSES  +   YFQ A+N++   +L+ AL   G+
Sbjct: 99  KKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLKQKTNLLGALTKAGI 158

Query: 158 APNGLSKSKQRVQSALRTHFGKEPVLRCRGTGS-NIRLIEIVMCFDDDGVTLINC 210
            P+G S S + ++ +++   G  P + C   GS N +L ++ +C D  G  LI C
Sbjct: 159 NPDGKSYSLESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDRSGSGLIEC 213

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BLAST of Moc09g12010 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 86.7 bits (213), Expect = 3.7e-16
Identity = 46/136 (33.82%), Postives = 75/136 (55.15%), Query Frame = 0


Query: 93  LPSISRRVWPNVI--TGDNKFFWSHEWNKHGVCSESKF-NMKAYFQMAINMRNSIDLMNA 152
           L S  ++ WP +   +G    FWSHEW KHG C+ES   N  AYF+ A++++N IDL++ 
Sbjct: 93  LISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCAESVLTNQHAYFKKALDLKNQIDLLSI 152

Query: 153 LKVGGLAPNGLSKSKQRVQSALRTHFGKEPVLRCR-GTGSNIRLIEIVMCFDDDGVTLIN 212
           L+   + P+G S     +++A+++  G  P ++C      N +L ++ +C D  G +LI 
Sbjct: 153 LQGADIHPDGESYDLVNIRNAIKSAIGYTPWIQCNVDQSGNSQLYQVYICVDGSGSSLIE 212

Query: 213 CNPV--SSNCANSFIF 223
           C P+     C  S  F
Sbjct: 213 C-PIFPGGKCGTSIEF 227

[Close]

BLAST of Moc09g12010 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 6.6e-13
Identity = 35/120 (29.17%), Postives = 63/120 (52.50%), Query Frame = 0


Query: 93  LPSISRRVWPNVI--TGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNAL 152
           L S  +R WP +   + D   FW+HEW KHG C+ES+ +   YF+  + ++   +L++AL
Sbjct: 86  LMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQKANLLHAL 145

Query: 153 KVGGLAPNGLSKSKQRVQSALRTHFGKEPVLRC-RGTGSNIRLIEIVMCFDDDGVTLINC 210
              G+ P+      + +++ ++   G  P + C + +  N +L +I +C D      INC
Sbjct: 146 TNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSHNSQLYQIYLCVDTSASKFINC 205

[Close]

BLAST of Moc09g12010 vs. ExPASy Swiss-Prot
Match: Q40381 (Ribonuclease S-7 OS=Nicotiana alata OX=4087 PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.5e-12
Identity = 37/115 (32.17%), Postives = 65/115 (56.52%), Query Frame = 0


Query: 101 WPNVITGD-----NKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGL 160
           WP++ T +     ++ FW HE+NKHG C    +N + YF +A+ +++  DL+++L+  G+
Sbjct: 89  WPDLTTAETYCKQHQNFWRHEYNKHGKCCSESYNREQYFDLAMALKDKFDLLSSLRNHGI 148

Query: 161 AP-NGLSKSKQRVQSALRTHFGKEPVLRCRGTGSNIRLIEIVMCFDDDGVTLINC 210
            P  G+  + Q++ S ++      P L C  T   + L+EI +CFD     +INC
Sbjct: 149 IPGRGMKYTVQKINSTIKKITQGYPNLSC--TKGIMELVEIGICFDSMVKNVINC 201

[Close]

BLAST of Moc09g12010 vs. ExPASy TrEMBL
Match: A0A6J1DQN7 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111022224 PE=3 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 8.2e-67
Identity = 124/124 (100.00%), Postives = 124/124 (100.00%), Query Frame = 0


Query: 99  RVWPNVITGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGLAPN 158
           RVWPNVITGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGLAPN
Sbjct: 93  RVWPNVITGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGLAPN 152

Query: 159 GLSKSKQRVQSALRTHFGKEPVLRCRGTGSNIRLIEIVMCFDDDGVTLINCNPVSSNCAN 218
           GLSKSKQRVQSALRTHFGKEPVLRCRGTGSNIRLIEIVMCFDDDGVTLINCNPVSSNCAN
Sbjct: 153 GLSKSKQRVQSALRTHFGKEPVLRCRGTGSNIRLIEIVMCFDDDGVTLINCNPVSSNCAN 212

Query: 219 SFIF 223
           SFIF
Sbjct: 213 SFIF 216

[Close]

BLAST of Moc09g12010 vs. ExPASy TrEMBL
Match: A0A6J1CKH7 (Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111012440 PE=3 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 2.8e-59
Identity = 107/124 (86.29%), Postives = 118/124 (95.16%), Query Frame = 0


Query: 99  RVWPNVITGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGLAPN 158
           RVWPNVITG+N+FFW HEWNKHG+CSESKF+MKAYFQMAINMR+S+DLMNAL+VGGLAPN
Sbjct: 97  RVWPNVITGNNRFFWGHEWNKHGICSESKFDMKAYFQMAINMRDSLDLMNALRVGGLAPN 156

Query: 159 GLSKSKQRVQSALRTHFGKEPVLRCRGTGSNIRLIEIVMCFDDDGVTLINCNPVSSNCAN 218
           G +KS+QRVQSA+R  FGKEPVLRCRGTG N+RLIEIVMCFDDDGVTLINCNP SSNCAN
Sbjct: 157 GRTKSRQRVQSAIRAQFGKEPVLRCRGTGRNVRLIEIVMCFDDDGVTLINCNPASSNCAN 216

Query: 219 SFIF 223
           SFIF
Sbjct: 217 SFIF 220

[Close]

BLAST of Moc09g12010 vs. ExPASy TrEMBL
Match: Q40115 (Ribonuclease (RNase LC1) OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 5.7e-52
Identity = 95/122 (77.87%), Postives = 109/122 (89.34%), Query Frame = 0


Query: 101 WPNVITGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGLAPNGL 160
           WPNVITG+NK+FW HEWNKHG+CS SKF+ +AYFQMAINMRNSIDL++AL+VGG+ PNG 
Sbjct: 93  WPNVITGNNKWFWGHEWNKHGICSVSKFDQQAYFQMAINMRNSIDLLSALRVGGVVPNGR 152

Query: 161 SKSKQRVQSALRTHFGKEPVLRCRGTGSNIRLIEIVMCFDDDGVTLINCNPVSSNCANSF 220
           SK++QRVQSA+R   GKEPVLRCRGTG   RL+EIVMCFDDDGVTLINCNP +SNC NSF
Sbjct: 153 SKARQRVQSAIRAQLGKEPVLRCRGTGRQSRLLEIVMCFDDDGVTLINCNPANSNCPNSF 212

Query: 221 IF 223
           IF
Sbjct: 213 IF 214

[Close]

BLAST of Moc09g12010 vs. ExPASy TrEMBL
Match: A0A0A0LC37 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 4.0e-45
Identity = 83/124 (66.94%), Postives = 102/124 (82.26%), Query Frame = 0


Query: 100 VWPNVITGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGLAPNG 159
           VWPNV+TG+NKFFW HEWNKHG+CSESKF+   YFQ AINMR+ IDL++ L+ GG+ PNG
Sbjct: 94  VWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKYFQTAINMRHGIDLLSVLRTGGVGPNG 153

Query: 160 LSKSKQRVQSALRTHFGKEPVLRC-RGTGSNIRLIEIVMCFDDDGVTLINCNPVSSNCAN 219
            SK+KQRV++A+ +HFGK+P+LRC + +   + L EIVMCFDDDGVTLINCN   SNCA 
Sbjct: 154 ASKAKQRVETAISSHFGKDPILRCKKASNGQVLLTEIVMCFDDDGVTLINCNKARSNCAG 213

Query: 220 SFIF 223
           SFIF
Sbjct: 214 SFIF 217

[Close]

BLAST of Moc09g12010 vs. ExPASy TrEMBL
Match: A0A5A7UJY4 (Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007930 PE=3 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 8.8e-45
Identity = 85/125 (68.00%), Postives = 104/125 (83.20%), Query Frame = 0


Query: 100 VWPNVITGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGLAPNG 159
           VWPNV+TG+NKFFW HEWNKHG+CSESKF+   YFQ AINMR+SIDL++ L+ GG+ PNG
Sbjct: 23  VWPNVVTGNNKFFWGHEWNKHGICSESKFDQAKYFQTAINMRHSIDLLSVLRTGGIVPNG 82

Query: 160 LSKSKQRVQSALRTHFGKEPVLRCRGTGSN--IRLIEIVMCFDDDGVTLINCNPVSSNCA 219
            SK+KQRV++A+ +HF K+P+LRC+ T SN  + L EIV+CFDDDGVTLINCN   SNCA
Sbjct: 83  ASKAKQRVETAISSHFAKDPILRCK-TASNGQVLLTEIVICFDDDGVTLINCNKARSNCA 142

Query: 220 NSFIF 223
            SFIF
Sbjct: 143 RSFIF 146

[Close]

BLAST of Moc09g12010 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 90.5 bits (223), Expect = 1.8e-18
Identity = 41/115 (35.65%), Postives = 65/115 (56.52%), Query Frame = 0


Query: 98  RRVWPNVI--TGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGL 157
           ++ WP +   +G  + FW HEW KHG CSES  +   YFQ A+N++   +L+ AL   G+
Sbjct: 99  KKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLKQKTNLLGALTKAGI 158

Query: 158 APNGLSKSKQRVQSALRTHFGKEPVLRCRGTGS-NIRLIEIVMCFDDDGVTLINC 210
            P+G S S + ++ +++   G  P + C   GS N +L ++ +C D  G  LI C
Sbjct: 159 NPDGKSYSLESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDRSGSGLIEC 213

[Close]

BLAST of Moc09g12010 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 75.9 bits (185), Expect = 4.7e-14
Identity = 35/120 (29.17%), Postives = 63/120 (52.50%), Query Frame = 0


Query: 93  LPSISRRVWPNVI--TGDNKFFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNAL 152
           L S  +R WP +   + D   FW+HEW KHG C+ES+ +   YF+  + ++   +L++AL
Sbjct: 86  LMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQKANLLHAL 145

Query: 153 KVGGLAPNGLSKSKQRVQSALRTHFGKEPVLRC-RGTGSNIRLIEIVMCFDDDGVTLINC 210
              G+ P+      + +++ ++   G  P + C + +  N +L +I +C D      INC
Sbjct: 146 TNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSHNSQLYQIYLCVDTSASKFINC 205

[Close]

BLAST of Moc09g12010 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 75.1 bits (183), Expect = 7.9e-14
Identity = 37/120 (30.83%), Postives = 61/120 (50.83%), Query Frame = 0


Query: 93  LPSISRRVWPNVITGDNK--FFWSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNAL 152
           L S  ++ WP +    N+   FW HEW KHG CSES  +   YF+ A+ ++   +L+  L
Sbjct: 92  LVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQHEYFENALKLKQKANLLQIL 151

Query: 153 KVGGLAPNGLSKSKQRVQSALRTHFGKEPVLRC-RGTGSNIRLIEIVMCFDDDGVTLINC 210
           K  G+ P+    +  ++ +A++   G  P + C +    N +L +I +C D  G   I C
Sbjct: 152 KNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNKDPERNAQLHQIYICVDTSGTEFIEC 211

[Close]

BLAST of Moc09g12010 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 69.7 bits (169), Expect = 3.3e-12
Identity = 34/112 (30.36%), Postives = 57/112 (50.89%), Query Frame = 0


Query: 113 WSHEWNKHGVCSESKFNMKAYFQMAINMRNSIDLMNALKVGGLAPNGLSKSKQRVQSALR 172
           W HEWNKHG C +S F+  +YF+  +  ++ + L+N L   G+ PN    S   +++A++
Sbjct: 114 WEHEWNKHGTCVQSIFDQHSYFRTNLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIK 173

Query: 173 THFGKEPVLRC-RGTGSNIRLIEIVMCFDDDGVTLINCNPV-SSNCANSFIF 223
              G  P + C      N +L +I +C D+     + C  V   +CA+   F
Sbjct: 174 CAIGFAPGIECNEDVKGNKQLFQIYICLDNYAKEFVECPYVPDKSCASKIKF 225

[Close]

BLAST of Moc09g12010 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 44.3 bits (103), Expect = 1.5e-04
Identity = 29/103 (28.16%), Postives = 47/103 (45.63%), Query Frame = 0


Query: 102 PNVITGDNKFFWSHEWNKHGVCSESKFNMK-AYFQMAINMRNSIDLMNAL-KVGGLAPNG 161
           P+   G    FW HEW KHG CS   F+ +  YF   +N+    ++ + L + G +A N 
Sbjct: 115 PSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVTDVLYQAGYVASNS 174

Query: 162 LSKSKQRVQSALRTHFGKEPVLRCRGTGSNIRLIEIVMCFDDD 203
                  + +A++  F   P + C+       + EI +CF  D
Sbjct: 175 EKYPLGGIVTAIQNAFHITPEVVCKRDA----IDEIRICFYKD 213

The following BLAST results are available for this feature:

BLAST of Moc09g12010 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155091.1	1.7e-66	100.00	ribonuclease MC-like [Momordica charantia]	[more]
XP_022142280.1	5.8e-59	86.29	ribonuclease MC-like [Momordica charantia]	[more]
BAA10891.1	1.2e-51	77.87	ribonuclease (RNase LC1) [Luffa aegyptiaca]	[more]
XP_038905534.1	2.1e-45	68.80	ribonuclease MC-like [Benincasa hispida]	[more]
XP_004147499.1	8.2e-45	66.94	ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [C...	[more]

BLAST of Moc09g12010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	1.1e-28	46.21	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P42813	2.6e-17	35.65	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P80022	3.7e-16	33.82	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P42815	6.6e-13	29.17	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
Q40381	1.5e-12	32.17	Ribonuclease S-7 OS=Nicotiana alata OX=4087 PE=1 SV=1	[more]

BLAST of Moc09g12010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DQN7	8.2e-67	100.00	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111022224 PE=3 SV=1	[more]
A0A6J1CKH7	2.8e-59	86.29	Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111012440 PE=3 SV=1	[more]
Q40115	5.7e-52	77.87	Ribonuclease (RNase LC1) OS=Luffa aegyptiaca OX=3670 PE=2 SV=1	[more]
A0A0A0LC37	4.0e-45	66.94	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1	[more]
A0A5A7UJY4	8.8e-45	68.00	Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352...	[more]

BLAST of Moc09g12010 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	1.8e-18	35.65	ribonuclease 1	[more]
AT1G26820.1	4.7e-14	29.17	ribonuclease 3	[more]
AT1G14220.1	7.9e-14	30.83	Ribonuclease T2 family protein	[more]
AT1G14210.1	3.3e-12	30.36	Ribonuclease T2 family protein	[more]
AT2G39780.1	1.5e-04	28.16	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 38..222 e-value: 1.7E-31 score: 111.9
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 99..222
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 98..209 e-value: 4.7E-24 score: 85.5
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 69..219
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 69..219
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 112..123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc09g12010.1	Moc09g12010.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006401	RNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding